Analysis of the human HSP70-HSP90 organising protein (HOP) gene - characterisation of the promoter and identification of a novel isoform
- Authors: Mattison, Stacey
- Date: 2018
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62821 , vital:28296
- Description: Expected release date-April 2020
- Full Text:
- Date Issued: 2018
- Authors: Mattison, Stacey
- Date: 2018
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62821 , vital:28296
- Description: Expected release date-April 2020
- Full Text:
- Date Issued: 2018
Antecedents and outcomes of service encounters in the restaurant industry in Zimbabwe
- Authors: Mukucha, Paul
- Date: 2018
- Subjects: Consumer behavior , Hospitality industry -- Zimbabwe -- Management Management -- Zimbabwe
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/32949 , vital:32479
- Description: This study aimed to determine the antecedents and outcomes of service encounter experiences of patrons in the restaurant industry in Zimbabwe. In addition, the study aimed to test the strength of the relationships between service encounter experiences and the selected antecedents, and outcomes. Service encounter experiences were investigated to contribute to the success of restaurants in Zimbabwe. The study presented literature on the restaurant industry in Zimbabwe. The literature explored the types of restaurant businesses that are operating in Zimbabwe, the contribution of the restaurant industry to the national economy, the attractiveness of the restaurant industry and the market structure of the restaurant industry. The literature also revealed that the restaurant industry immensely contributes to the national economy of Zimbabwe through employment creation and statutory payments. It was further revealed that the restaurant industry in Zimbabwe is attractive because there are little entry and exit barriers, and there is some degree of differentiation of service delivery. The contemporary issues that affect the restaurant industry in Zimbabwe cut across several macro business factors, and were identified as political, economic, social, technological, legal and environmental factors. Theories and literature were considered, and the study extensively presented content related to the postulated relationships among the study’s variables, namely the specified antecedents of service encounter experiences which are the variables service contact levels, ambience factors, service delivery quality, and typology of patrons; and the selected outcomes of service encounter experiences, namely patronage retention, re-patronage retention, and patronage loyalty. A synthesis of how all the study constructs are interrelated, culminating with the proposed conceptual model, were presented. The proposed conceptual model that captures service encounter experiences and its possible antecedents and outcomes, depicted a series of hypotheses. The hypotheses presented were developed by using extant theoretical and empirical literature. The study further presented the research methodology, and the results of this study. This explanatory study followed a quantitative survey research design and 400 restaurant patrons in Zimbabwe were respondents to the study’s questionnaire. Descriptive statistics were calculated. In addition, using exploratory factor analyses results, the conceptual framework was updated, and further inferential statistics were computed. These statistics included a reliability analysis, correlation analysis and regression analyses to statistically test the proposed relationships. The empirical results revealed that all the postulated independent variables, except the variable service contact levels, are positively and significantly related to the patrons’ service encounter experiences. Furthermore, the study revealed that the patrons’ service encounter experiences significantly influence two specified dependent variables namely re-patronage intention and patronage loyalty. Patronage retention, which was one of the hypothesised outcomes of service encounter experiences, was eliminated from the proposed conceptual model during the measurement scale validation phase as almost all of its items loaded onto the patronage loyalty factor. This suggested the absence of a conceptual distinction between patronage retention and patronage loyalty. The study concluded that the levels of the patrons’ service encounter experiences in the restaurant industry in Zimbabwe are high as a result of higher levels of the postulated antecedents such as ambience factors, service delivery quality and typology of patrons. However, the levels of contact in restaurants did not have any significant impact on the patrons’ service encounter experiences. The study also concluded that eliciting pleasant service encounter experiences generates higher levels of re-patronage retention and patronage loyalty. The conclusions generated in this study subsequently led to a number of recommendations for restaurants in Zimbabwe. The study also contributed by either closing or clarifying many research gaps as well as by developing a hypothetical model and measuring instrument. This study was the first to show how ambience factors, service quality delivery and the typology of patrons, based on their personality traits, could positively influence the service encounter experiences of Zimbabwean restaurants’ patrons. Furthermore, the study confirms that in the restaurant industry, the patronage retention construct is conceptually the same as the patronage loyalty construct. Moreover, restaurant businesses must exploit the differences in their patrons’ dispositions in order to enhance service encounter experiences through providing differentiated service offerings.
- Full Text:
- Date Issued: 2018
- Authors: Mukucha, Paul
- Date: 2018
- Subjects: Consumer behavior , Hospitality industry -- Zimbabwe -- Management Management -- Zimbabwe
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/32949 , vital:32479
- Description: This study aimed to determine the antecedents and outcomes of service encounter experiences of patrons in the restaurant industry in Zimbabwe. In addition, the study aimed to test the strength of the relationships between service encounter experiences and the selected antecedents, and outcomes. Service encounter experiences were investigated to contribute to the success of restaurants in Zimbabwe. The study presented literature on the restaurant industry in Zimbabwe. The literature explored the types of restaurant businesses that are operating in Zimbabwe, the contribution of the restaurant industry to the national economy, the attractiveness of the restaurant industry and the market structure of the restaurant industry. The literature also revealed that the restaurant industry immensely contributes to the national economy of Zimbabwe through employment creation and statutory payments. It was further revealed that the restaurant industry in Zimbabwe is attractive because there are little entry and exit barriers, and there is some degree of differentiation of service delivery. The contemporary issues that affect the restaurant industry in Zimbabwe cut across several macro business factors, and were identified as political, economic, social, technological, legal and environmental factors. Theories and literature were considered, and the study extensively presented content related to the postulated relationships among the study’s variables, namely the specified antecedents of service encounter experiences which are the variables service contact levels, ambience factors, service delivery quality, and typology of patrons; and the selected outcomes of service encounter experiences, namely patronage retention, re-patronage retention, and patronage loyalty. A synthesis of how all the study constructs are interrelated, culminating with the proposed conceptual model, were presented. The proposed conceptual model that captures service encounter experiences and its possible antecedents and outcomes, depicted a series of hypotheses. The hypotheses presented were developed by using extant theoretical and empirical literature. The study further presented the research methodology, and the results of this study. This explanatory study followed a quantitative survey research design and 400 restaurant patrons in Zimbabwe were respondents to the study’s questionnaire. Descriptive statistics were calculated. In addition, using exploratory factor analyses results, the conceptual framework was updated, and further inferential statistics were computed. These statistics included a reliability analysis, correlation analysis and regression analyses to statistically test the proposed relationships. The empirical results revealed that all the postulated independent variables, except the variable service contact levels, are positively and significantly related to the patrons’ service encounter experiences. Furthermore, the study revealed that the patrons’ service encounter experiences significantly influence two specified dependent variables namely re-patronage intention and patronage loyalty. Patronage retention, which was one of the hypothesised outcomes of service encounter experiences, was eliminated from the proposed conceptual model during the measurement scale validation phase as almost all of its items loaded onto the patronage loyalty factor. This suggested the absence of a conceptual distinction between patronage retention and patronage loyalty. The study concluded that the levels of the patrons’ service encounter experiences in the restaurant industry in Zimbabwe are high as a result of higher levels of the postulated antecedents such as ambience factors, service delivery quality and typology of patrons. However, the levels of contact in restaurants did not have any significant impact on the patrons’ service encounter experiences. The study also concluded that eliciting pleasant service encounter experiences generates higher levels of re-patronage retention and patronage loyalty. The conclusions generated in this study subsequently led to a number of recommendations for restaurants in Zimbabwe. The study also contributed by either closing or clarifying many research gaps as well as by developing a hypothetical model and measuring instrument. This study was the first to show how ambience factors, service quality delivery and the typology of patrons, based on their personality traits, could positively influence the service encounter experiences of Zimbabwean restaurants’ patrons. Furthermore, the study confirms that in the restaurant industry, the patronage retention construct is conceptually the same as the patronage loyalty construct. Moreover, restaurant businesses must exploit the differences in their patrons’ dispositions in order to enhance service encounter experiences through providing differentiated service offerings.
- Full Text:
- Date Issued: 2018
Anthropogenic effects on sustainability of fish biodiversity in Tyume River, Eastern Cape, South Africa
- Authors: Kinya, Jane Njeri
- Date: 2018
- Subjects: Fishes--Behavior Fishes--Physiology Biotic communities
- Language: English
- Type: Thesis , Doctoral , Zoology
- Identifier: http://hdl.handle.net/10353/11740 , vital:39102
- Description: To determine the anthropogenic effects on the sustainability of fish biodiversity in Tyhume River, a mixture of ecological, economic and institutional parameters were used. To measure ecological parameters, 10 study sites were selected to represent varying intensities of anthropogenic effects on habitat, to represent typical river zones, and to correspond with historical survey sites for trend analysis. In these study sites, habitat characteristics that represent geomorphology (habitat quantity), water quality and ‘alien’ (non–native) fishes were used to determine anthropogenic effects on habitat and fish assemblage. The measured geomorphology characteristics included current velocity, wetted width and depth. The water quality characteristics used were temperature, pH and conductivity. To determine the effects of native and non-native on indigenous fish species in situ, electro fishing was used in riffles, small pools and runs; as well, in situ seine-netting was done in pools. The riches of river fish species was measured, using numbers abundance and longitudinal distribution indices. It was established that only 11percent of the Tyhume River habitat was relatively near natural, while 89percent exhibited anthropogenic habitat modification. Three major sources ofanthropogenic modification on habitat were identified. The major contributor of anthropogenic effects was Binfield Park Dam which accounted for 43percent of modification. The second source of anthropogenic effects on habitat was Alice urban area where storm water runoff accounted for 28percent of modification. Agricultural and livestock grazing, the third anthropogenic effect, accounted for 18percent of modification. The Eastern Cape Rocky, Sandelia bainsii Castelnau, 1861 and Border Barb, Barbus trevelyani Günther, 1868, exhibited reduced distribution. B. trevelyani longitudinal distribution was less than 25 km along the river continuum, with sporadic presence in the sandy foothills. S. bainsii was not found in the Lowland Zone, while previously this species extended from the mountainous zone to the confluence of the Tyhume and Keiskamma Rivers in the Lowland Zone. These indices supported the homogenization theory, as reflected by increased dissimilarity for two indigenous fish species, S. bainsii and B. trevelyani, among study sites. S. bainsii was previously distributed from source to confluence, while B. trevelyani recorded a 50percent decline at the study site below the Binfield Park Dam. To assign economic value to ecosystem services and assess post Rio-institutional parameters two hypothetical scenarios were developed from the ecological survey; one depicting current conditions and another depicting improved ecosystem services. Using a structured interview questionnaire, five ecosystem services in need of restoration were described to respondents. Applying the contingent valuation method respondents were asked a dichotomous choice question on willingness to pay (WTP). Results of the economic study from 209 personal interviews revealed, individuals were willing to pay an additional R 32.00 on their monthly water bill or R 384.00 annually. Generalizing this to 2 829 households living in the Alice and Ntselamanzi urban areas, a total value of R 1 086 336 would be realized annually. This was equivalent to the Nkonkobe Municipality annual budget for storm water management in Alice urban area. Costs/benefits revealed a positive net present value (NPV) = 1, which, in line with economic theory, meant gainers were able to compensate losers The institutional part of the economic survey sought to determine the effects of post Rio Earth Summit institutional measures on sustainable management of Tyhume River fish assemblage. A desk review of Rio Declaration and three South African statutes were used namely; Constitution Act 108 of 1996 (South African Constitution 1996); the National Environmental Management Biodiversity Act No. 10 of 2004 (NEMBA 2004), the National Environmental Management Act (NEMA) No. 107 of 1998 (NEMA 1998), and the National Water Act No. 36 of 1998 (NWA 1998). The Institutional and Analytic Development (IAD) Framework, coupled with requirements for sustainability of Multiple Use Resource Domains were used for the analysis. De Jure, the statutes had internationalization of environmental costs through “polluter pays principle”, while sustainable use was the overarching goal. De facto, the community participation, a requisite of post Rio measures, was low; however 89percent of community respondents were willing to join an environmental conservation group. This, coupled with the fact that 77percent of those interviewed supported the establishment of a Tyhume River Restoration Fund, provided scope for a broad based community participation framework This study contributes to sustainable use of Tyhume River and other lotic systems by generating information on the link between anthropogenic effects on fish biodiversity, economic value of ecosystems services and institutional mechanisms.
- Full Text:
- Date Issued: 2018
- Authors: Kinya, Jane Njeri
- Date: 2018
- Subjects: Fishes--Behavior Fishes--Physiology Biotic communities
- Language: English
- Type: Thesis , Doctoral , Zoology
- Identifier: http://hdl.handle.net/10353/11740 , vital:39102
- Description: To determine the anthropogenic effects on the sustainability of fish biodiversity in Tyhume River, a mixture of ecological, economic and institutional parameters were used. To measure ecological parameters, 10 study sites were selected to represent varying intensities of anthropogenic effects on habitat, to represent typical river zones, and to correspond with historical survey sites for trend analysis. In these study sites, habitat characteristics that represent geomorphology (habitat quantity), water quality and ‘alien’ (non–native) fishes were used to determine anthropogenic effects on habitat and fish assemblage. The measured geomorphology characteristics included current velocity, wetted width and depth. The water quality characteristics used were temperature, pH and conductivity. To determine the effects of native and non-native on indigenous fish species in situ, electro fishing was used in riffles, small pools and runs; as well, in situ seine-netting was done in pools. The riches of river fish species was measured, using numbers abundance and longitudinal distribution indices. It was established that only 11percent of the Tyhume River habitat was relatively near natural, while 89percent exhibited anthropogenic habitat modification. Three major sources ofanthropogenic modification on habitat were identified. The major contributor of anthropogenic effects was Binfield Park Dam which accounted for 43percent of modification. The second source of anthropogenic effects on habitat was Alice urban area where storm water runoff accounted for 28percent of modification. Agricultural and livestock grazing, the third anthropogenic effect, accounted for 18percent of modification. The Eastern Cape Rocky, Sandelia bainsii Castelnau, 1861 and Border Barb, Barbus trevelyani Günther, 1868, exhibited reduced distribution. B. trevelyani longitudinal distribution was less than 25 km along the river continuum, with sporadic presence in the sandy foothills. S. bainsii was not found in the Lowland Zone, while previously this species extended from the mountainous zone to the confluence of the Tyhume and Keiskamma Rivers in the Lowland Zone. These indices supported the homogenization theory, as reflected by increased dissimilarity for two indigenous fish species, S. bainsii and B. trevelyani, among study sites. S. bainsii was previously distributed from source to confluence, while B. trevelyani recorded a 50percent decline at the study site below the Binfield Park Dam. To assign economic value to ecosystem services and assess post Rio-institutional parameters two hypothetical scenarios were developed from the ecological survey; one depicting current conditions and another depicting improved ecosystem services. Using a structured interview questionnaire, five ecosystem services in need of restoration were described to respondents. Applying the contingent valuation method respondents were asked a dichotomous choice question on willingness to pay (WTP). Results of the economic study from 209 personal interviews revealed, individuals were willing to pay an additional R 32.00 on their monthly water bill or R 384.00 annually. Generalizing this to 2 829 households living in the Alice and Ntselamanzi urban areas, a total value of R 1 086 336 would be realized annually. This was equivalent to the Nkonkobe Municipality annual budget for storm water management in Alice urban area. Costs/benefits revealed a positive net present value (NPV) = 1, which, in line with economic theory, meant gainers were able to compensate losers The institutional part of the economic survey sought to determine the effects of post Rio Earth Summit institutional measures on sustainable management of Tyhume River fish assemblage. A desk review of Rio Declaration and three South African statutes were used namely; Constitution Act 108 of 1996 (South African Constitution 1996); the National Environmental Management Biodiversity Act No. 10 of 2004 (NEMBA 2004), the National Environmental Management Act (NEMA) No. 107 of 1998 (NEMA 1998), and the National Water Act No. 36 of 1998 (NWA 1998). The Institutional and Analytic Development (IAD) Framework, coupled with requirements for sustainability of Multiple Use Resource Domains were used for the analysis. De Jure, the statutes had internationalization of environmental costs through “polluter pays principle”, while sustainable use was the overarching goal. De facto, the community participation, a requisite of post Rio measures, was low; however 89percent of community respondents were willing to join an environmental conservation group. This, coupled with the fact that 77percent of those interviewed supported the establishment of a Tyhume River Restoration Fund, provided scope for a broad based community participation framework This study contributes to sustainable use of Tyhume River and other lotic systems by generating information on the link between anthropogenic effects on fish biodiversity, economic value of ecosystems services and institutional mechanisms.
- Full Text:
- Date Issued: 2018
AP-MOVPE growth and characterisation of GaSb/GaAs quantum dots
- Authors: Tile, Ngcali
- Date: 2018
- Subjects: Quantum theory , Quantum electronics Quantum dots Semiconductors
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/36716 , vital:34047
- Description: GaSb/GaAs quantum dots (QD) were grown by atmospheric pressure (AP) metal-organic vapour phase epitaxy (MOVPE) using triethylgallium (TEGa), tertiarybutylarsine (tBAs) and trimethylantimony (TMSb) as gallium (Ga), arsenic (As) and (Sb) sources, respectively. The effect of AP-MOVPE growth parameters on the formation of GaSb QD structures on GaAs was studied. The formation of small, coherent GaSb dots on GaAs improved with decreasing V/III ratios, which were controlled through changing either the TMSb/TEGa ratio at a constant growth temperature or changing the growth temperature at a constant TMSb/TEGa ratio. The maximum effective V/III ratio for dot formation was 0.175. The dot density was more sensitive to growth time than to source mole fraction in the reactor, since time determines the amount of deposited material. The dot density increased with increasing growth time, while the shape and size of the dots were more sensitive to the source vapour mole fraction, which controls the growth rate. Lower mole fractions resulted in smaller sized dots with a more uniform distribution compared to higher mole fractions. Dome-shaped dots with densities as high as 4×1010 cm-2, average base length of 35 nm and average height of 5 nm were achieved. Capping of GaSb QDs at high temperatures caused flattening and the formation of a thin, inhomogeneous GaSb layer inside GaAs. No obvious QD photoluminescence (PL) peak was detected for these samples. A two stage process for capping the dots (involving growth of a low temperature GaAs cap, followed by a high temperature cap) led to the retention of the dot-like features in/on a wetting layer (WL) of GaSb and distinct PL peaks for both the QDs and WL. An increase in excitation power during PL measurements for this particular sample caused the QD and WL peaks to shift to higher energies. This is attributed to electrostatic band bending, leading to triangular potential wells, typical for type II band alignment between GaAs and strained GaSb. Variable temperature PL measurements showed the decrease in the intensity of the WL peak to be faster than that of the QD peak as the measurement temperature increased. A detailed high resolution transmission electron microscopy analysis was performed to study the morphology and chemical interaction between GaAs and GaSb regions for capped GaSb/GaAs QDs. The capped dots had dimensions similar to those of uncapped dots and had a higher concentration of Sb at their center, with the periphery being intermixed with GaAs. Measurement of lattice strain performed inside these dots revealed the strain to be distributed inhomogenously throughout the dot area. The effect of GaAs host matrix on excitonic behaviour in AP-MOVPE grown GaSb/GaAs quantum dots was investigated. Room temperature (RT) PL emission was achieved from a single layer of quantum dots by controlling the GaAs host matrix growth temperature. These samples were prepared using a GaSb dot growth temperature of 530 °C, followed by growth of a thin GaAs ‘cold’ cap, before depositing the final part of the GaAs capping layer at either 550 °C, 600 °C or 650 °C. PL measurements at 10 K revealed QD emission peaks for all the samples at around 1.1 eV. However, variable temperature PL revealed different thermal quenching rates of the emission, with the rates of quenching reduced with increasing GaAs growth temperature. This was ascribed to reduced defect densities in GaAs grown at higher temperature, which resulted in QD emission even at RT. This RT emission peaked at approximately 1 eV. The hole localisation energy determined for these samples at RT was approximately 470 meV.
- Full Text:
- Date Issued: 2018
- Authors: Tile, Ngcali
- Date: 2018
- Subjects: Quantum theory , Quantum electronics Quantum dots Semiconductors
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/36716 , vital:34047
- Description: GaSb/GaAs quantum dots (QD) were grown by atmospheric pressure (AP) metal-organic vapour phase epitaxy (MOVPE) using triethylgallium (TEGa), tertiarybutylarsine (tBAs) and trimethylantimony (TMSb) as gallium (Ga), arsenic (As) and (Sb) sources, respectively. The effect of AP-MOVPE growth parameters on the formation of GaSb QD structures on GaAs was studied. The formation of small, coherent GaSb dots on GaAs improved with decreasing V/III ratios, which were controlled through changing either the TMSb/TEGa ratio at a constant growth temperature or changing the growth temperature at a constant TMSb/TEGa ratio. The maximum effective V/III ratio for dot formation was 0.175. The dot density was more sensitive to growth time than to source mole fraction in the reactor, since time determines the amount of deposited material. The dot density increased with increasing growth time, while the shape and size of the dots were more sensitive to the source vapour mole fraction, which controls the growth rate. Lower mole fractions resulted in smaller sized dots with a more uniform distribution compared to higher mole fractions. Dome-shaped dots with densities as high as 4×1010 cm-2, average base length of 35 nm and average height of 5 nm were achieved. Capping of GaSb QDs at high temperatures caused flattening and the formation of a thin, inhomogeneous GaSb layer inside GaAs. No obvious QD photoluminescence (PL) peak was detected for these samples. A two stage process for capping the dots (involving growth of a low temperature GaAs cap, followed by a high temperature cap) led to the retention of the dot-like features in/on a wetting layer (WL) of GaSb and distinct PL peaks for both the QDs and WL. An increase in excitation power during PL measurements for this particular sample caused the QD and WL peaks to shift to higher energies. This is attributed to electrostatic band bending, leading to triangular potential wells, typical for type II band alignment between GaAs and strained GaSb. Variable temperature PL measurements showed the decrease in the intensity of the WL peak to be faster than that of the QD peak as the measurement temperature increased. A detailed high resolution transmission electron microscopy analysis was performed to study the morphology and chemical interaction between GaAs and GaSb regions for capped GaSb/GaAs QDs. The capped dots had dimensions similar to those of uncapped dots and had a higher concentration of Sb at their center, with the periphery being intermixed with GaAs. Measurement of lattice strain performed inside these dots revealed the strain to be distributed inhomogenously throughout the dot area. The effect of GaAs host matrix on excitonic behaviour in AP-MOVPE grown GaSb/GaAs quantum dots was investigated. Room temperature (RT) PL emission was achieved from a single layer of quantum dots by controlling the GaAs host matrix growth temperature. These samples were prepared using a GaSb dot growth temperature of 530 °C, followed by growth of a thin GaAs ‘cold’ cap, before depositing the final part of the GaAs capping layer at either 550 °C, 600 °C or 650 °C. PL measurements at 10 K revealed QD emission peaks for all the samples at around 1.1 eV. However, variable temperature PL revealed different thermal quenching rates of the emission, with the rates of quenching reduced with increasing GaAs growth temperature. This was ascribed to reduced defect densities in GaAs grown at higher temperature, which resulted in QD emission even at RT. This RT emission peaked at approximately 1 eV. The hole localisation energy determined for these samples at RT was approximately 470 meV.
- Full Text:
- Date Issued: 2018
Application of health belief model in developing contextual guidelines for adherence to antiretroviral treatment among adolescents and youths in Buffalo City Metropolitan Municipality District, East London, South Africa
- Authors: Mayeye, Bulelwa
- Date: 2018
- Subjects: HIV-positive children--Treatment--Management AIDS (Disease) in adolescence HIV-positive youth
- Language: English
- Type: Thesis , Doctoral , Nursing
- Identifier: http://hdl.handle.net/10353/15572 , vital:40472
- Description: Background: Adhering to the treatment instructions for a long-term illness poses a great challenge to the client especially adolescents and youths. In public health centers it is observed that most adolescents and youths on Ante Retroviral Treatment (ART) do not continue taking ART and do not honor their Art related scheduled appointments at the clinic. An increase loss to follow up among these cohorts is noticeable in Buffalo City Metropolitan Municipality district. Inconsistent adherence to ART may result in the development of resistance mutations. Purpose: The purpose of the study was to develop and describe context – specific antiretroviral treatment guidelines based on the biomedical, cognitive and psychosocial needs of the adolescents and youths at the Buffalo City Metropolitan Municipality district. Methodology: The study was conducted in two phases, phase one consisted of quantitative and qualitative approaches. For phase one an explanatory sequential mixed method of quantitative and qualitative designs was employed. The study was guided by Health Belief Model. The target population were adolescents and youths receiving ART from the selected health facilities in Buffalo City Metropolitan Municipality District in the Province of the Eastern Cape. Participants were chosen based on purposive sampling. A convenience sample of 206 adolescents and youths between the ages of 14 to 24 attending ARV clinics were approached. Ethical considerations, that is, the rights of all the participants were honoured. Data was collected using self administered questionnaires followed by semi-structured interview where face to face interviews were conducted. Reliability validity and trustworthiness of the research was ensured. Quantitative data was analysed through descriptive statistics. For qualitative approach transcrips were coded thematically. In Phase 2, context – specific antiretroviral treatment guidelines were developed to promote adherence to ART by adolescents and youths of Buffalo City Metropolitan Municipality district. Results: About 35percent of the participants indicated low adherence, 31percent for moderate adherence and 34percent for high adherence. A strong correlation between interpersonal relationship and adherence (p = 0.008), a correlation between aspect of care related to clinic and adherence (p = 0.028), a significant correlation between social support and adherence to ART (p = 0.006) and a significant relationship between self- efficacy and adherence to ART (p = 0.000) were obtained. No correlation between demographic status and adherence except for race. Results from the interviews revealed that barriers to adherence were more evident than enabling factors. Recommendations: Health service providers should strengthen ART adherence programmes for adolescents and youths by applying principles as they appear in the context – based guidelines.
- Full Text:
- Date Issued: 2018
- Authors: Mayeye, Bulelwa
- Date: 2018
- Subjects: HIV-positive children--Treatment--Management AIDS (Disease) in adolescence HIV-positive youth
- Language: English
- Type: Thesis , Doctoral , Nursing
- Identifier: http://hdl.handle.net/10353/15572 , vital:40472
- Description: Background: Adhering to the treatment instructions for a long-term illness poses a great challenge to the client especially adolescents and youths. In public health centers it is observed that most adolescents and youths on Ante Retroviral Treatment (ART) do not continue taking ART and do not honor their Art related scheduled appointments at the clinic. An increase loss to follow up among these cohorts is noticeable in Buffalo City Metropolitan Municipality district. Inconsistent adherence to ART may result in the development of resistance mutations. Purpose: The purpose of the study was to develop and describe context – specific antiretroviral treatment guidelines based on the biomedical, cognitive and psychosocial needs of the adolescents and youths at the Buffalo City Metropolitan Municipality district. Methodology: The study was conducted in two phases, phase one consisted of quantitative and qualitative approaches. For phase one an explanatory sequential mixed method of quantitative and qualitative designs was employed. The study was guided by Health Belief Model. The target population were adolescents and youths receiving ART from the selected health facilities in Buffalo City Metropolitan Municipality District in the Province of the Eastern Cape. Participants were chosen based on purposive sampling. A convenience sample of 206 adolescents and youths between the ages of 14 to 24 attending ARV clinics were approached. Ethical considerations, that is, the rights of all the participants were honoured. Data was collected using self administered questionnaires followed by semi-structured interview where face to face interviews were conducted. Reliability validity and trustworthiness of the research was ensured. Quantitative data was analysed through descriptive statistics. For qualitative approach transcrips were coded thematically. In Phase 2, context – specific antiretroviral treatment guidelines were developed to promote adherence to ART by adolescents and youths of Buffalo City Metropolitan Municipality district. Results: About 35percent of the participants indicated low adherence, 31percent for moderate adherence and 34percent for high adherence. A strong correlation between interpersonal relationship and adherence (p = 0.008), a correlation between aspect of care related to clinic and adherence (p = 0.028), a significant correlation between social support and adherence to ART (p = 0.006) and a significant relationship between self- efficacy and adherence to ART (p = 0.000) were obtained. No correlation between demographic status and adherence except for race. Results from the interviews revealed that barriers to adherence were more evident than enabling factors. Recommendations: Health service providers should strengthen ART adherence programmes for adolescents and youths by applying principles as they appear in the context – based guidelines.
- Full Text:
- Date Issued: 2018
Appraisal of wastewater final effluents and river water as reservoirs of cholera and non-cholera causing vibrio species : case study of the Amatole and OR Tambo District Municipalities
- Authors: Nontongana, Nolonwabo
- Date: 2018
- Subjects: Vibrio -- South Africa -- Eastern Cape Vibrio cholerae Disease Reservoirs
- Language: English
- Type: Thesis , Doctoral , Microbiology
- Identifier: http://hdl.handle.net/10353/9929 , vital:35146
- Description: Vibrio infections remain a serious threat to public health. In the last decade, Vibrio disease outbreaks have created a painful awareness of the personal, economic, societal, and public health costs associated with the impact of inadequately treated wastewater effluents. This study was therefore designed to assess the occurrence of cholera and non-cholera causing Vibrio species in the final effluents of wastewater treatment plants and river waters in the Amatole (BT WWTP) and OR Tambo District municipalities (MT WWTP and QN River) of the Eastern Cape Province. Samples were collected monthly from December 2016 to November 2017 from the final effluent, 500 meters upstream and downstream of the discharge points and analysed for physicochemical parameters, Vibrio pathogens prevalence and their antibiogram characteristics using both culture-based and molecular techniques. Samples were collected aseptically using sterile 1L glass bottles containing 0.5 ml of sterile sodium thiosulphate solution and transported on ice to the laboratory for analyses within 6hrs of collection. The membrane filtration method was used for enumeration of presumptive Vibrio densities on thiosulfate citrate bile salt (TCBS) agar plates. Polymerase chain reaction (PCR) was then used to confirm the identities of the presumptive Vibrio species using the species-specific primers. The confirmed isolates were further subjected to molecular characterization to confirm their respective pathotypes. Antimicrobial susceptibility testing was done by the standard disc diffusion method recommended by the Clinical and Laboratory Standards Institute. The recovered Vibrio species were tested against a panel of 17 antibiotics. Physicochemical parameters measured include pH, temperature, electrical conductivity, salinity, turbidity, total dissolved solid (TDS), dissolved oxygen (DO), biochemical oxygen demand (BOD) and chlorine (only for BT WWTP). Unacceptably high levels of the assayed parameters were observed in many cases for TDS (66 - 879 mg/l), turbidity (2.0 -722.33 NTU) and DO (2.5 – 9.7 mg/l) as well as chlorine (0.2 – 3.2 mg/L). Presumptive Vibrio densities varied from 2.91 to 3.91 log10 CFU/100 ml and 2.67 to 3.18 log CFU/100ml, for BT WWTP and MT WWTP respectively. The densities for the QN River ranged between 2.51 to 3.99 log10 CFU/100mL. Out of 720 presumptive isolates recovered, 619 (86 percent) were found to be positive for the Vibrio genus. Molecular confirmation of the presumptive Vibrio species revealed the presence of V. fluvialis (16), V. vulnificus (12), V aliginolyticus (9), V. parahaemolyticus (37) and V. cholerae (5) isolates were confirmed. The susceptibility against 17 different antibiotics by the recovered species were examined. V. cholerae was notably resistant against nalidixic acid (3) and Ampicilin (2), all the V. vulnificus isolates were resistant against ampicillin (16), V. fluvialis showed resistance against ciprofloxacin and nalidixic acid, all (9) isolates for V. alginolyticus were resistant against ampicillin. V. parahaemolyticus showed resistance against cefutoxime (16), cefuxime (8) and ampicillin (13). The recovery of Vibrio in the discharged effluents throughout the sampling period even in adequately disinfected effluents is not acceptable considering that Vibrio are pathogenic bacteria. The findings of this study underline the need for constant monitoring of the physicochemical and microbiological qualities of discharged effluents and might also be suggestive for a review of the disinfection methods used at the treatment works as this might pose adverse health risk to the communities which still rely heavily on these surface waters.
- Full Text:
- Date Issued: 2018
- Authors: Nontongana, Nolonwabo
- Date: 2018
- Subjects: Vibrio -- South Africa -- Eastern Cape Vibrio cholerae Disease Reservoirs
- Language: English
- Type: Thesis , Doctoral , Microbiology
- Identifier: http://hdl.handle.net/10353/9929 , vital:35146
- Description: Vibrio infections remain a serious threat to public health. In the last decade, Vibrio disease outbreaks have created a painful awareness of the personal, economic, societal, and public health costs associated with the impact of inadequately treated wastewater effluents. This study was therefore designed to assess the occurrence of cholera and non-cholera causing Vibrio species in the final effluents of wastewater treatment plants and river waters in the Amatole (BT WWTP) and OR Tambo District municipalities (MT WWTP and QN River) of the Eastern Cape Province. Samples were collected monthly from December 2016 to November 2017 from the final effluent, 500 meters upstream and downstream of the discharge points and analysed for physicochemical parameters, Vibrio pathogens prevalence and their antibiogram characteristics using both culture-based and molecular techniques. Samples were collected aseptically using sterile 1L glass bottles containing 0.5 ml of sterile sodium thiosulphate solution and transported on ice to the laboratory for analyses within 6hrs of collection. The membrane filtration method was used for enumeration of presumptive Vibrio densities on thiosulfate citrate bile salt (TCBS) agar plates. Polymerase chain reaction (PCR) was then used to confirm the identities of the presumptive Vibrio species using the species-specific primers. The confirmed isolates were further subjected to molecular characterization to confirm their respective pathotypes. Antimicrobial susceptibility testing was done by the standard disc diffusion method recommended by the Clinical and Laboratory Standards Institute. The recovered Vibrio species were tested against a panel of 17 antibiotics. Physicochemical parameters measured include pH, temperature, electrical conductivity, salinity, turbidity, total dissolved solid (TDS), dissolved oxygen (DO), biochemical oxygen demand (BOD) and chlorine (only for BT WWTP). Unacceptably high levels of the assayed parameters were observed in many cases for TDS (66 - 879 mg/l), turbidity (2.0 -722.33 NTU) and DO (2.5 – 9.7 mg/l) as well as chlorine (0.2 – 3.2 mg/L). Presumptive Vibrio densities varied from 2.91 to 3.91 log10 CFU/100 ml and 2.67 to 3.18 log CFU/100ml, for BT WWTP and MT WWTP respectively. The densities for the QN River ranged between 2.51 to 3.99 log10 CFU/100mL. Out of 720 presumptive isolates recovered, 619 (86 percent) were found to be positive for the Vibrio genus. Molecular confirmation of the presumptive Vibrio species revealed the presence of V. fluvialis (16), V. vulnificus (12), V aliginolyticus (9), V. parahaemolyticus (37) and V. cholerae (5) isolates were confirmed. The susceptibility against 17 different antibiotics by the recovered species were examined. V. cholerae was notably resistant against nalidixic acid (3) and Ampicilin (2), all the V. vulnificus isolates were resistant against ampicillin (16), V. fluvialis showed resistance against ciprofloxacin and nalidixic acid, all (9) isolates for V. alginolyticus were resistant against ampicillin. V. parahaemolyticus showed resistance against cefutoxime (16), cefuxime (8) and ampicillin (13). The recovery of Vibrio in the discharged effluents throughout the sampling period even in adequately disinfected effluents is not acceptable considering that Vibrio are pathogenic bacteria. The findings of this study underline the need for constant monitoring of the physicochemical and microbiological qualities of discharged effluents and might also be suggestive for a review of the disinfection methods used at the treatment works as this might pose adverse health risk to the communities which still rely heavily on these surface waters.
- Full Text:
- Date Issued: 2018
Approaches to detecting and assessing patterns, processes and responses to change in South African estuaries
- Authors: Van Niekerk, Lara
- Date: 2018
- Subjects: Estuaries -- South Africa , Climatic changes -- South Africa
- Language: English
- Type: Thesis , Doctoral , PHD
- Identifier: http://hdl.handle.net/10948/45491 , vital:38621
- Description: The research presented indicates deep knowledge of estuary pattern-process-function across a broad range of estuary types. This study successfully developed and applied approaches to detect change at varying temporal and spatial scales in South African estuaries. This assessment was based on the physical characteristics of estuaries and predicted or measured change in the drivers of estuary health. This is the first study in the country to review and synthesise the vulnerability of South African estuaries to Climate Change on a regional scale. Through sound scientific approaches this research has advanced our knowledge on complex estuarine systems and made a significant contribution globally to approaches for the conservation and management of estuaries.
- Full Text:
- Date Issued: 2018
- Authors: Van Niekerk, Lara
- Date: 2018
- Subjects: Estuaries -- South Africa , Climatic changes -- South Africa
- Language: English
- Type: Thesis , Doctoral , PHD
- Identifier: http://hdl.handle.net/10948/45491 , vital:38621
- Description: The research presented indicates deep knowledge of estuary pattern-process-function across a broad range of estuary types. This study successfully developed and applied approaches to detect change at varying temporal and spatial scales in South African estuaries. This assessment was based on the physical characteristics of estuaries and predicted or measured change in the drivers of estuary health. This is the first study in the country to review and synthesise the vulnerability of South African estuaries to Climate Change on a regional scale. Through sound scientific approaches this research has advanced our knowledge on complex estuarine systems and made a significant contribution globally to approaches for the conservation and management of estuaries.
- Full Text:
- Date Issued: 2018
Aspects Allocentristes de l’Humanisme dans l’Univers Romanesque de Williams Sassine
- Authors: Dami, Emmanuel Naancin
- Date: 2018
- Subjects: Sassine, Williams, 1944-1997 -- Criticism and interpretation , Guinean fiction (French) -- 20th century -- History and criticism , Humanism in literature , Human beings in literature , Postcolonialism in literature , Existentialism in literature
- Language: French , English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61957 , vital:28089
- Description: The human being, his behaviour and more importantly, his very essence have, for time immemorial, proven to be enigmatic to many a man and to researchers in particular. Consequently, the need for a thorough and more profound understanding of man has made him the epicentre of epistemic investigations across many academic disciplines. This, unfortunately, does not seem to have shaded bright lights on the grey areas bordering on his nature to his behavior. This, in turn, opens up other windows requiring further probes. For instance, proponents of the Neo-classical economic school contend that the human being is homo oeconomicus. This implies that man is intrinsically egocentric and a rational being whose decisions are based on informed choices driven by his urge for maximal utility geared towards his personal satisfaction (O’Boyle 2009; Caruso 2012). This stance invalidates man’s ability to do ‘disinterested good’ in any form. African francophone literature is replete with different and diverse images of man, his behaviour and existential conditions: sufferings and struggles. It, therefore, constitutes, inter alia, a laboratory in which researchers undertake investigative endeavours. This thesis beams its floodlights on the allocentric aspects of humanism in the novels of Williams Sassine. Thus, using the phenomenological and ‘clavieniste’ lenses as overarching operational theories, the thesis investigates Sassine’s novels with the view to shading a ray of light on the ‘humanistic man’ especially the allocentric dimensions of his nature and comportment. Emphasis is laid on the motives and motivations underlying the individual and the in-group struggle of the sassinien heroes in the emancipation of their society from the shackles of colonial and postcolonial domination, exploitation and subjugation resulting in self-determination and the subsequent disenchantment. Furthermore, the thesis examines the nature and character of human charity, generosity and sacrifices among the characters in the novels. In conclusion, the thesis establishes, through an x-ray of the nature of actions and decisions of certain characters in the novels, the examination of the conscious and subconscious intentions underlying these same actions and decisions which, on a face value, are apparently altruistic, that the sassinien heroes’ efforts and sacrifices are geared towards the common and greater good. This invariably means that man is, indeed, capable of posing disinterested acts of charity and bringing succour, devoid of any ulterior motives for personal gain, to his fellow humans. The position of the Neo-classical economists is consequently over-assuming, sweeping and tad generalizing. The sassinien characters have demonstrated beyond reasonable doubt that they are humanists. Their nature, the remote and immediate causes of their fights, the goals and objectives of their struggles and their spirit of abnegation have indeed proven that “human beings have the right and the responsibility to give meaning and shape to their own lives. [They have stood] for the building of a more humane society through an ethics based on the human and other natural values in a spirit of reason and free inquiry through human capabilities” (AHA 2017 : 3).
- Full Text:
- Date Issued: 2018
- Authors: Dami, Emmanuel Naancin
- Date: 2018
- Subjects: Sassine, Williams, 1944-1997 -- Criticism and interpretation , Guinean fiction (French) -- 20th century -- History and criticism , Humanism in literature , Human beings in literature , Postcolonialism in literature , Existentialism in literature
- Language: French , English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61957 , vital:28089
- Description: The human being, his behaviour and more importantly, his very essence have, for time immemorial, proven to be enigmatic to many a man and to researchers in particular. Consequently, the need for a thorough and more profound understanding of man has made him the epicentre of epistemic investigations across many academic disciplines. This, unfortunately, does not seem to have shaded bright lights on the grey areas bordering on his nature to his behavior. This, in turn, opens up other windows requiring further probes. For instance, proponents of the Neo-classical economic school contend that the human being is homo oeconomicus. This implies that man is intrinsically egocentric and a rational being whose decisions are based on informed choices driven by his urge for maximal utility geared towards his personal satisfaction (O’Boyle 2009; Caruso 2012). This stance invalidates man’s ability to do ‘disinterested good’ in any form. African francophone literature is replete with different and diverse images of man, his behaviour and existential conditions: sufferings and struggles. It, therefore, constitutes, inter alia, a laboratory in which researchers undertake investigative endeavours. This thesis beams its floodlights on the allocentric aspects of humanism in the novels of Williams Sassine. Thus, using the phenomenological and ‘clavieniste’ lenses as overarching operational theories, the thesis investigates Sassine’s novels with the view to shading a ray of light on the ‘humanistic man’ especially the allocentric dimensions of his nature and comportment. Emphasis is laid on the motives and motivations underlying the individual and the in-group struggle of the sassinien heroes in the emancipation of their society from the shackles of colonial and postcolonial domination, exploitation and subjugation resulting in self-determination and the subsequent disenchantment. Furthermore, the thesis examines the nature and character of human charity, generosity and sacrifices among the characters in the novels. In conclusion, the thesis establishes, through an x-ray of the nature of actions and decisions of certain characters in the novels, the examination of the conscious and subconscious intentions underlying these same actions and decisions which, on a face value, are apparently altruistic, that the sassinien heroes’ efforts and sacrifices are geared towards the common and greater good. This invariably means that man is, indeed, capable of posing disinterested acts of charity and bringing succour, devoid of any ulterior motives for personal gain, to his fellow humans. The position of the Neo-classical economists is consequently over-assuming, sweeping and tad generalizing. The sassinien characters have demonstrated beyond reasonable doubt that they are humanists. Their nature, the remote and immediate causes of their fights, the goals and objectives of their struggles and their spirit of abnegation have indeed proven that “human beings have the right and the responsibility to give meaning and shape to their own lives. [They have stood] for the building of a more humane society through an ethics based on the human and other natural values in a spirit of reason and free inquiry through human capabilities” (AHA 2017 : 3).
- Full Text:
- Date Issued: 2018
Aspects of the nutritional physiology of the perlemoen Haliotis midae (L.) and red abalone H. rufescens (Swainson)
- Authors: Kemp, Justin Oliver Gordon
- Date: 2018
- Subjects: Haliotis midae , Haliotis midae fisheries , Red abalone , Haliotis midae -- Feeding and feeds , Red abalone -- Feeding and feeds , Haliotis midae -- Nutrition -- Requirements , Red abalone -- Nutrition -- Requirements , Haliotis midae -- Metabolism , Red abalone -- Metabolism , Haliotis midae -- Physiology , Red abalone -- Physiology , Stable isotopes , Algae as feed
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62314 , vital:28154
- Description: The source of abalone for human consumption has shown a dramatic shift away from wild-capture fisheries in the last 30 years, with over 90% of global production now coming from aquaculture. Farmers initially relied on the natural food of abalone (macroalgae) as a culture feed, though in regions where macroalgae availability was limiting, the need to develop formulated feeds was evident. Extensive research effort has led to the development of a number of formulated feed products currently employed in the industry. These feeds, however, differ markedly from the mixed macroalgal diets that abalone have evolved to utilise, particularly in terms of protein content and carbohydrate structure. The degree to which the nutritional physiology of abalone responds to these novel formulated diets, with and without macroalgal supplementation, was investigated in the current study. A multifaceted approach, combining growth trials, stable isotope nutrient tracers and metabolic experiments, was employed to gain insight into the post-absorption dynamics and utilisation of dietary nutrients under varying dietary regimes of fresh macroalgae and formulated feed. Growth trials conducted with both Haliotis rufescens and H. midae showed significantly higher growth and protein utilisation efficiency for abalone fed macroalgal diets compared to formulated feeds. Furthermore, when formulated feeds were supplemented with macroalgae to form combination diets, growth and the utilization of protein was improved compared to the formulated-feed-only diet. The poor utilisation of protein by H. midae fed the formulated feed could be traced, using a method combining stable isotope bio-markers with a Bayesian mixing model (SIAR), to the low incorporation of the fishmeal component of protein in the diet. The marked postprandial drop in the O:N ratio on abalone fed formulated feeds indicate that the protein was being diverted into catabolic metabolic pathways. The metabolic cost of digestion, termed specific dynamic action (SDA), was negated as a factor in the improved growth of abalone fed macroalgal diets, with the SDA coefficient 2.1 times that observed for formulated feed. Furthermore, the postprandial haemolymph glucose concentration (HGC) in H. midae was elevated when fed formulated feed compared to macroalgae. The high levels of circulating glucose are likely a result of the structure the carbohydrate source in formulated feeds and stimulate the deposition of glycogen through the allosteric control of glycogen synthase. Formulated feeds produced higher cooked meat yields in canning simulation trials, suggesting that muscle glycogen content may indirectly play a role in increasing canning yields through the displacement of collagen. The results of these empirical studies are synthesised under key themes, discussed within the context of their potential commercial relevance and future research directions are highlighted.
- Full Text:
- Date Issued: 2018
- Authors: Kemp, Justin Oliver Gordon
- Date: 2018
- Subjects: Haliotis midae , Haliotis midae fisheries , Red abalone , Haliotis midae -- Feeding and feeds , Red abalone -- Feeding and feeds , Haliotis midae -- Nutrition -- Requirements , Red abalone -- Nutrition -- Requirements , Haliotis midae -- Metabolism , Red abalone -- Metabolism , Haliotis midae -- Physiology , Red abalone -- Physiology , Stable isotopes , Algae as feed
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62314 , vital:28154
- Description: The source of abalone for human consumption has shown a dramatic shift away from wild-capture fisheries in the last 30 years, with over 90% of global production now coming from aquaculture. Farmers initially relied on the natural food of abalone (macroalgae) as a culture feed, though in regions where macroalgae availability was limiting, the need to develop formulated feeds was evident. Extensive research effort has led to the development of a number of formulated feed products currently employed in the industry. These feeds, however, differ markedly from the mixed macroalgal diets that abalone have evolved to utilise, particularly in terms of protein content and carbohydrate structure. The degree to which the nutritional physiology of abalone responds to these novel formulated diets, with and without macroalgal supplementation, was investigated in the current study. A multifaceted approach, combining growth trials, stable isotope nutrient tracers and metabolic experiments, was employed to gain insight into the post-absorption dynamics and utilisation of dietary nutrients under varying dietary regimes of fresh macroalgae and formulated feed. Growth trials conducted with both Haliotis rufescens and H. midae showed significantly higher growth and protein utilisation efficiency for abalone fed macroalgal diets compared to formulated feeds. Furthermore, when formulated feeds were supplemented with macroalgae to form combination diets, growth and the utilization of protein was improved compared to the formulated-feed-only diet. The poor utilisation of protein by H. midae fed the formulated feed could be traced, using a method combining stable isotope bio-markers with a Bayesian mixing model (SIAR), to the low incorporation of the fishmeal component of protein in the diet. The marked postprandial drop in the O:N ratio on abalone fed formulated feeds indicate that the protein was being diverted into catabolic metabolic pathways. The metabolic cost of digestion, termed specific dynamic action (SDA), was negated as a factor in the improved growth of abalone fed macroalgal diets, with the SDA coefficient 2.1 times that observed for formulated feed. Furthermore, the postprandial haemolymph glucose concentration (HGC) in H. midae was elevated when fed formulated feed compared to macroalgae. The high levels of circulating glucose are likely a result of the structure the carbohydrate source in formulated feeds and stimulate the deposition of glycogen through the allosteric control of glycogen synthase. Formulated feeds produced higher cooked meat yields in canning simulation trials, suggesting that muscle glycogen content may indirectly play a role in increasing canning yields through the displacement of collagen. The results of these empirical studies are synthesised under key themes, discussed within the context of their potential commercial relevance and future research directions are highlighted.
- Full Text:
- Date Issued: 2018
Aspects of the population ecology, habitat use and behaviour of the endangered Knysna seahorse (Hippocampus capensis Boulenger, 1900) in a residential marina estate, Knysna, South Africa: implications for conservation
- Authors: Claassens, Louw
- Date: 2018
- Subjects: Endangered species -- South Africa -- Knysna , Sea horses -- Behavior -- South Africa -- Knysna , Sea horses -- Habitat -- South Africa -- Knysna , Sea horses -- Ecology -- South Africa -- Knysna
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/54789 , vital:26616
- Description: The Knysna seahorse Hippocampus capensis is South Africa’s only endemic seahorse species, and is found in only three adjacent estuaries along the southern coast. The conservation of this endangered species is important on a national and international level. This study presents the first research on this species within the Knysna estuary since 2001 and specifically focuses on aspects of its ecology within a residential marina estate (Thesen Islands Marina). The physico-chemical and habitat features of the marina were described and the population ecology, habitat use, and behaviour of the Knysna seahorse were investigated. Physico-chemical conditions within the western section of the marina, characterised by high water current velocities, were similar to that of the adjacent estuary. The eastern section of the marina was characterised by lower water current velocities and higher turbidity. Four major habitat types were identified within the marina canals: (I) artificial Reno mattress (wire baskets filled with rocks); (II) Codium tenue beds; (III) mixed vegetation on sediment; and (IV) barren canal floor. Seahorse densities within the marina were significantly higher compared to densities found historically within the estuary. Highest seahorse densities were specifically found within the artificial Reno mattress structures and within the western section of the marina. Seahorse density varied spatially and temporally and the type of habitat was an important predictor for seahorse occurrence. An experimental investigation found that H. capensis chooses artificial Reno mattress habitat over Zostera capensis when given a choice. GoPro cameras were used successfully to investigate daytime seahorse behaviour within the Reno mattress habitat. Seahorses were more active during the morning, spent most of their time (> 80 %) feeding, and morning courting behaviour for this species were confirmed. However, during the summer holiday period (mid-December to mid-January) few seahorses were observed on camera, which suggests that the increase in motor boat activity and the related increase in noise had a negative effect on H. capensis feeding and courting behaviour. The marina development, and in particular the Reno mattresses, created a new habitat for this endangered species within the Knysna estuary. In addition to the protection and restoration of natural habitats in which H. capensis is found, the conservation potential of artificial structures such as Reno mattresses should be realised.
- Full Text:
- Date Issued: 2018
- Authors: Claassens, Louw
- Date: 2018
- Subjects: Endangered species -- South Africa -- Knysna , Sea horses -- Behavior -- South Africa -- Knysna , Sea horses -- Habitat -- South Africa -- Knysna , Sea horses -- Ecology -- South Africa -- Knysna
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/54789 , vital:26616
- Description: The Knysna seahorse Hippocampus capensis is South Africa’s only endemic seahorse species, and is found in only three adjacent estuaries along the southern coast. The conservation of this endangered species is important on a national and international level. This study presents the first research on this species within the Knysna estuary since 2001 and specifically focuses on aspects of its ecology within a residential marina estate (Thesen Islands Marina). The physico-chemical and habitat features of the marina were described and the population ecology, habitat use, and behaviour of the Knysna seahorse were investigated. Physico-chemical conditions within the western section of the marina, characterised by high water current velocities, were similar to that of the adjacent estuary. The eastern section of the marina was characterised by lower water current velocities and higher turbidity. Four major habitat types were identified within the marina canals: (I) artificial Reno mattress (wire baskets filled with rocks); (II) Codium tenue beds; (III) mixed vegetation on sediment; and (IV) barren canal floor. Seahorse densities within the marina were significantly higher compared to densities found historically within the estuary. Highest seahorse densities were specifically found within the artificial Reno mattress structures and within the western section of the marina. Seahorse density varied spatially and temporally and the type of habitat was an important predictor for seahorse occurrence. An experimental investigation found that H. capensis chooses artificial Reno mattress habitat over Zostera capensis when given a choice. GoPro cameras were used successfully to investigate daytime seahorse behaviour within the Reno mattress habitat. Seahorses were more active during the morning, spent most of their time (> 80 %) feeding, and morning courting behaviour for this species were confirmed. However, during the summer holiday period (mid-December to mid-January) few seahorses were observed on camera, which suggests that the increase in motor boat activity and the related increase in noise had a negative effect on H. capensis feeding and courting behaviour. The marina development, and in particular the Reno mattresses, created a new habitat for this endangered species within the Knysna estuary. In addition to the protection and restoration of natural habitats in which H. capensis is found, the conservation potential of artificial structures such as Reno mattresses should be realised.
- Full Text:
- Date Issued: 2018
Assessing the Contribution of SMMEs to Job creation in the Eastern Cape South Africa
- Authors: Mbambo, William Bongile
- Date: 2018
- Subjects: Business enterprises -- South Africa -- Eastern Cape Job creation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD ( Public Administration)
- Identifier: http://hdl.handle.net/10353/13675 , vital:39690
- Description: Job creation through support to Small, Medium and Micro Enterprises (SMMEs) is one of the South African government’s priorities in the Eastern Cape Vision 2030 Provincial Development Plan, to overcome the chronic unemployment situation faced by millions of South Africans. The Eastern Cape Province is amongst one of the areas challenged by high levels of unemployment in South Africa. In order to assess whether SMMEs had the capacity to create employment opportunities in the Eastern Cape Province. This study investigate on how SMMEs plans to provide employment opportunities, also examine whether SMMEs has created any employment opportunities thus far in the Eastern Cape Province. The study used David Birch (1979 and 1981) Kerr, Wittenberg and Arrow (2013), theories to assess whether SMMEs had the capacity to create employment opportunities in the Province. The study adopted a mixed methods approach in order to assess SMMEs as a tool for employment creation in its various dimensions. The results of the study reveal that Small, Medium and Micro Enterprises do not necessarily generate substantial employment. Therefore, the study recommends that the government should provide more financial support for SMMEs, organize entrepreneurship workshops, seminars and training workshops, which could improve their business operating skills as well as provide easy access to loans.
- Full Text:
- Date Issued: 2018
- Authors: Mbambo, William Bongile
- Date: 2018
- Subjects: Business enterprises -- South Africa -- Eastern Cape Job creation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD ( Public Administration)
- Identifier: http://hdl.handle.net/10353/13675 , vital:39690
- Description: Job creation through support to Small, Medium and Micro Enterprises (SMMEs) is one of the South African government’s priorities in the Eastern Cape Vision 2030 Provincial Development Plan, to overcome the chronic unemployment situation faced by millions of South Africans. The Eastern Cape Province is amongst one of the areas challenged by high levels of unemployment in South Africa. In order to assess whether SMMEs had the capacity to create employment opportunities in the Eastern Cape Province. This study investigate on how SMMEs plans to provide employment opportunities, also examine whether SMMEs has created any employment opportunities thus far in the Eastern Cape Province. The study used David Birch (1979 and 1981) Kerr, Wittenberg and Arrow (2013), theories to assess whether SMMEs had the capacity to create employment opportunities in the Province. The study adopted a mixed methods approach in order to assess SMMEs as a tool for employment creation in its various dimensions. The results of the study reveal that Small, Medium and Micro Enterprises do not necessarily generate substantial employment. Therefore, the study recommends that the government should provide more financial support for SMMEs, organize entrepreneurship workshops, seminars and training workshops, which could improve their business operating skills as well as provide easy access to loans.
- Full Text:
- Date Issued: 2018
Assessment of the host potential of TETROL [(+)-(2R,3R)-1,1,4,4- tetraphenylbutane-1,2,3,4-TETROL] for the separation of isomers and related compounds
- Authors: Dorfling, Sasha-Lee
- Date: 2018
- Subjects: Chemistry, Organic , Thermal analysis Hydrogen bonding
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/22060 , vital:29817
- Description: In this study, we investigated the potential of a host compound, (+)-(2R,3R)-1,1,4,4- tetraphenylbutane-1,2,3,4-tetrol (TETROL), for use in the separation of isomers and related compounds using host-guest chemistry. The synthesis of this host was carried out using a standard Grignard procedure, reacting naturally-occurring optically active tartaric acid with phenylmagnesium bromide. The feasibility of this host for separating isomers and structurally-related compounds was investigated by recrystallizing it from various potential cyclic, aromatic and aliphatic guest compounds. The extent of host inclusion and guest separation were determined using 1H-NMR spectroscopy and GCMS analyses. Competition studies were conducted to establish the selectivity of TETROL for the various guest species and whether this host would be able to discriminate between them. In this instance, the host was recrystallized from equimolar amounts of binary, ternary, quaternary or quinary mixtures of the guests present in each target study. Subsequent binary or ternary competitions were conducted where the molar ratios of the guest species were varied beyond equimolar, and the guest selectivity of TETROL thus evaluated by means of selectivity profiles. Further analyses included single crystal X-ray diffraction (SCXRD), thermal analysis and Hirshfeld surface analysis. Any crystalline inclusion complex formed between host and guest, with suitable crystal quality, was analysed using SCXRD in order to determine the nature of any significant host–guest interactions present. Thermogravimetric and differential scanning calorimetry experiments provided further insight into complex stability by analysing the thermal events experienced by the complexes as they were heated at 10 °C/min. The data obtained from Hirshfeld surface analyses were used to determine whether host selectivity and/or thermal stability of the complexes were related to the number and types of interactions, observed from SCXRD, between host and guest. The ability of TETROL to discriminate between related compounds was favourable. This host proved to have selective preference for aniline over its methylated derivatives, N-methylaniline and N,N-dimethylaniline. It was also selective for cyclohexylamine over cyclohexanol and cyclohexanone, and discriminated against the pyridine, piperidine and dioxane heterocyclics in favour of morpholine. Furthermore, this host was successful in the selective separation of isomers; for example, it selectively showed discrimination between the three toluidine isomers (p-toluidine > m-toluidine > o-toluidine) and the cresols (p-cresol > m-cresol > o-cresol). Each guest mixture was selected based on data from experiments using either the industrial significance of its separation or because the mixture would add to the knowledge base of the host compound’s preferences and selectivities. In a separate study, TETROL and its derivative, (–)-(2R,3R)-2,3-dimethoxy-1,1,4,4- tetraphenylbutane-1,4-diol (DMT), were also allowed to compete for the inclusion of the guest cyclohexanone, where TETROL demonstrated superior ability. This host, in addition, showed potential for the separation of cis- and trans- 2-methylcyclohexanol.
- Full Text:
- Date Issued: 2018
- Authors: Dorfling, Sasha-Lee
- Date: 2018
- Subjects: Chemistry, Organic , Thermal analysis Hydrogen bonding
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/22060 , vital:29817
- Description: In this study, we investigated the potential of a host compound, (+)-(2R,3R)-1,1,4,4- tetraphenylbutane-1,2,3,4-tetrol (TETROL), for use in the separation of isomers and related compounds using host-guest chemistry. The synthesis of this host was carried out using a standard Grignard procedure, reacting naturally-occurring optically active tartaric acid with phenylmagnesium bromide. The feasibility of this host for separating isomers and structurally-related compounds was investigated by recrystallizing it from various potential cyclic, aromatic and aliphatic guest compounds. The extent of host inclusion and guest separation were determined using 1H-NMR spectroscopy and GCMS analyses. Competition studies were conducted to establish the selectivity of TETROL for the various guest species and whether this host would be able to discriminate between them. In this instance, the host was recrystallized from equimolar amounts of binary, ternary, quaternary or quinary mixtures of the guests present in each target study. Subsequent binary or ternary competitions were conducted where the molar ratios of the guest species were varied beyond equimolar, and the guest selectivity of TETROL thus evaluated by means of selectivity profiles. Further analyses included single crystal X-ray diffraction (SCXRD), thermal analysis and Hirshfeld surface analysis. Any crystalline inclusion complex formed between host and guest, with suitable crystal quality, was analysed using SCXRD in order to determine the nature of any significant host–guest interactions present. Thermogravimetric and differential scanning calorimetry experiments provided further insight into complex stability by analysing the thermal events experienced by the complexes as they were heated at 10 °C/min. The data obtained from Hirshfeld surface analyses were used to determine whether host selectivity and/or thermal stability of the complexes were related to the number and types of interactions, observed from SCXRD, between host and guest. The ability of TETROL to discriminate between related compounds was favourable. This host proved to have selective preference for aniline over its methylated derivatives, N-methylaniline and N,N-dimethylaniline. It was also selective for cyclohexylamine over cyclohexanol and cyclohexanone, and discriminated against the pyridine, piperidine and dioxane heterocyclics in favour of morpholine. Furthermore, this host was successful in the selective separation of isomers; for example, it selectively showed discrimination between the three toluidine isomers (p-toluidine > m-toluidine > o-toluidine) and the cresols (p-cresol > m-cresol > o-cresol). Each guest mixture was selected based on data from experiments using either the industrial significance of its separation or because the mixture would add to the knowledge base of the host compound’s preferences and selectivities. In a separate study, TETROL and its derivative, (–)-(2R,3R)-2,3-dimethoxy-1,1,4,4- tetraphenylbutane-1,4-diol (DMT), were also allowed to compete for the inclusion of the guest cyclohexanone, where TETROL demonstrated superior ability. This host, in addition, showed potential for the separation of cis- and trans- 2-methylcyclohexanol.
- Full Text:
- Date Issued: 2018
Assessment of the human health implications of climate variability in East London, Eastern Cape, South Africa
- Authors: Orimoloye, Israel Ropo
- Date: 2018
- Subjects: Climatic changes -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , Geography
- Identifier: http://hdl.handle.net/10353/10113 , vital:35346
- Description: Impacts associated with climate variability and extreme heat are already obvious in varying degrees and expected to be disruptive in the near future across the globe especially in the urban regions. Urban areas have distinctive features that leave their residents and properties vulnerable to extreme climate events. Global temperatures continue to change, reaching new levels almost every year for the past two decades. However, even though the causes are debated it is evident that climate variability is real. Climate variability and disaster risk are threats to human health that adversely reinforce each other. Better knowledge on the association between climate change, variability and extreme weather-related illness is needed and can aid strategies to reduce vulnerabilities. The impacts of climate variability on the health of residents in East London (EL) area in the Eastern Cape Province, South Africa were explored through four interdependent research segments. The first section examined the climate variability and urban surface thermal characteristics implication on human health using Remote Sensing (RS) and Geographic Information System (GIS) techniques. Remote sensing was used to assess the Land Surface Temperature (LST) and estimated Radiation (R) of East London area from Landsat Thematic Mapper (TM) images for 1986, 1996, 2006 as well as from Operational Land Imager (OLI) and Thermal Infrared Sensor (TIRS) for 2016 spanning a period of 30 years. Rapid urbanization and land cover changes in this area have contributed significantly to this drastic change in the natural land surface characteristics (increased land surface temperature and surface solar radiation). For instance, vegetation cover declined by about 358.812km2 while built-up areas increased by 175.473km2 during this period which correlates with the area thermal characteristics changes. Radiation levels also increased over the years with values exceeding the global solar radiation index. Exposure to increased surface radiation poses risks of heat stroke, skin cancer and heart disease to the local population. Consequently, this study provides pertinent information on human health sustainability and epidemiological case management. The second part explored past temperature and humidity trends (1986-2016) and projects future trends (2017-2030). The historical data of meteorological variables were obtained from the archives of the South African Weather Service and analyzed using the ordinary least square regression model in GRETL (GNU Regression Econometric and Time-series Library) statistical software. This study discovered a local consistency between models and the observations add to existing knowledge and this is crucial in knowing the shifts in climatic change as well as recognizing variability and its conflicting effects on human health, environment, agriculture, ecological sustainability and socioeconomic status in the region. The third segment assessed the potential impacts of climate variability on health using existing heat indices during the study period. The results demonstrated that in East London from 1986 to 2016 during summer and autumn (December to May) of various years exceeded high heat index values. It is obvious that summer and autumn months are more vulnerable to heat extreme and humidex. The humidex and Heat Index (HI) increased annually by 0.03percent and 0.9percent respectively throughout the study period. The increment in the various indices showed highly significant ill-health and environmental impacts on humans especially with prolonged exposure. The last segment appraised the association between climatic elements and epidemiological incidences of the study area between 2012 and 2016. The epidemiology incidences data were obtained from the archives of the Cecilia Makiwane Hospital in East London area and National Tertiary Service Grant (NTSG) database for the period. The results have showed that there exists significant effects of climate variability on the health of East London residents and these have been identified to have negative impacts on health of the people in the area. This study also revealed noticeable impacts of extreme heat on human health and a positive correlation between meteorological components (HI and temperature) and epidemiological cases (cardiovascular, skin cancer and diarrhea) during the study period.
- Full Text:
- Date Issued: 2018
- Authors: Orimoloye, Israel Ropo
- Date: 2018
- Subjects: Climatic changes -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , Geography
- Identifier: http://hdl.handle.net/10353/10113 , vital:35346
- Description: Impacts associated with climate variability and extreme heat are already obvious in varying degrees and expected to be disruptive in the near future across the globe especially in the urban regions. Urban areas have distinctive features that leave their residents and properties vulnerable to extreme climate events. Global temperatures continue to change, reaching new levels almost every year for the past two decades. However, even though the causes are debated it is evident that climate variability is real. Climate variability and disaster risk are threats to human health that adversely reinforce each other. Better knowledge on the association between climate change, variability and extreme weather-related illness is needed and can aid strategies to reduce vulnerabilities. The impacts of climate variability on the health of residents in East London (EL) area in the Eastern Cape Province, South Africa were explored through four interdependent research segments. The first section examined the climate variability and urban surface thermal characteristics implication on human health using Remote Sensing (RS) and Geographic Information System (GIS) techniques. Remote sensing was used to assess the Land Surface Temperature (LST) and estimated Radiation (R) of East London area from Landsat Thematic Mapper (TM) images for 1986, 1996, 2006 as well as from Operational Land Imager (OLI) and Thermal Infrared Sensor (TIRS) for 2016 spanning a period of 30 years. Rapid urbanization and land cover changes in this area have contributed significantly to this drastic change in the natural land surface characteristics (increased land surface temperature and surface solar radiation). For instance, vegetation cover declined by about 358.812km2 while built-up areas increased by 175.473km2 during this period which correlates with the area thermal characteristics changes. Radiation levels also increased over the years with values exceeding the global solar radiation index. Exposure to increased surface radiation poses risks of heat stroke, skin cancer and heart disease to the local population. Consequently, this study provides pertinent information on human health sustainability and epidemiological case management. The second part explored past temperature and humidity trends (1986-2016) and projects future trends (2017-2030). The historical data of meteorological variables were obtained from the archives of the South African Weather Service and analyzed using the ordinary least square regression model in GRETL (GNU Regression Econometric and Time-series Library) statistical software. This study discovered a local consistency between models and the observations add to existing knowledge and this is crucial in knowing the shifts in climatic change as well as recognizing variability and its conflicting effects on human health, environment, agriculture, ecological sustainability and socioeconomic status in the region. The third segment assessed the potential impacts of climate variability on health using existing heat indices during the study period. The results demonstrated that in East London from 1986 to 2016 during summer and autumn (December to May) of various years exceeded high heat index values. It is obvious that summer and autumn months are more vulnerable to heat extreme and humidex. The humidex and Heat Index (HI) increased annually by 0.03percent and 0.9percent respectively throughout the study period. The increment in the various indices showed highly significant ill-health and environmental impacts on humans especially with prolonged exposure. The last segment appraised the association between climatic elements and epidemiological incidences of the study area between 2012 and 2016. The epidemiology incidences data were obtained from the archives of the Cecilia Makiwane Hospital in East London area and National Tertiary Service Grant (NTSG) database for the period. The results have showed that there exists significant effects of climate variability on the health of East London residents and these have been identified to have negative impacts on health of the people in the area. This study also revealed noticeable impacts of extreme heat on human health and a positive correlation between meteorological components (HI and temperature) and epidemiological cases (cardiovascular, skin cancer and diarrhea) during the study period.
- Full Text:
- Date Issued: 2018
Assessment of the impact of land use practices on vegetation , soil and carbon-nitrogen sequestration potential in Mopane rangelands of Central Northern Namibia
- Authors: Kahumba, Absalom
- Date: 2018
- Subjects: Rural development Land use Land tenure
- Language: English
- Type: Thesis , Doctoral , Pasture Science
- Identifier: http://hdl.handle.net/10353/11718 , vital:39100
- Description: This study was conducted in Ogongo district, situated in Omusati Region of the central northern Namibia. The objectives of this study were to assess the impact of land use practices on 1) vegetation distribution and status; 2) soil properties; 3) carbon-nitrogen sequestration potential, and 4) investigate the indigenous pastoralists’ knowledge and perceptions on livestock production, rangeland degradation and its indicators in three villages (Omaandi, Epukunoyana and Oshitutuma) in Mopane savannah rangelands of central northern Namibia. For vegetation and soil assessment, three grazing land management systems (communal, game reserve and cattle ranch) were identified adjacent to each other. In each grazing system, three sites (for game reserve and cattle ranch, these were camps) were selected. In each site, three belt transects of 50 m x 5 m2 were marked to record vegetation and soil data as well as to collect vegetation samples for analysis. Grass species composition was recorded using step point methods from 250 points per belt transect. A total of five 0.25 m2 quadrats were randomly laid within each 250 m2 belt transect to record the herbaceous plant density, tuft diameter, tuft distance and biomass. All woody plants in the belt transect (250 m2) were measured and recorded for species plant height and canopy diameters. Three soil samples per belt transect were collected at the time of forage sampling down to a depth of 20 cm using a soil auger. Soil and plant samples were analysed for macro and micro minerals, total organic carbon, total nitrogen, carbon-nitrogen ratios, and carbon and nitrogen isotopes. For bulk density, soil samples were collected using soil core samplers. In the social study, a total of 90 households were randomly selected from three villages and a total of 48 elders were purposefully selected based on the age, time spent in the village and livestock ownership. A combination of structured and semi-structured questionnaires were used to gather household information, while a structured questionnaire and open ended discussion were used to interview elders on the constraints of livestock production, rangeland degradation related issues and their consequences on livelihood. Field vegetation and soil data were analyzed using a General Linear Model (GLM) in SAS (2007), whereas the social data were analyzed using SPSS version 21 (2014). A total of 23 grass species were identified in all three land management systems, of which 48% were perennials and the remaining 52% were annuals. Aristida meridionalis dominated the game reserve and the ranch, whereas Eragrostis trichophora was dominant in all land management systems. Forbs occurred commonly in the game reserve and the ranch, but were dominant in the communal areas. The abundance of A. meridionalis responded significantly (P < 0.001) to land management systems with the game reserve showing remarkably greater abundance than the ranch. In summer, the abundance of E. trichophora was high (P < 0.001) in the game reserve followed by the communal and ranch sites, whereas in winter, the species had still greater abundance in the game reserve followed by the ranch. In summer, the mean grass density was greatest (P < 0.001) in the game reserve and least in the communal lands, but vice versa for the tuft distance. In summer, the average distance between tufts showed great variations (P < 0.001) with the communal areas having greater values than the ranch and game reserve. In winter, both the game reserve and the ranch showed records of similar grass tuft density and distance between tufts, whereas these values were nil for communal areas, because, there were no grasses recorded. Herbaceous plant dry matter was significantly greater (P < 0.05) in the game reserve followed by the ranch and the least in the communal areas. Results on biomass showed remarkably higher values (P < 0.001) in summer than winter in all land management systems. The total woody plant density was significantly higher (P < 0.001) in the ranch (542 TE ha-1) than the game reserve (449 TE ha-1) and communal area (324 TE ha-1). However, percentage of canopy cover did not differ significantly (P > 0.05) between the three land management systems. Woody plant density of height classes were significantly different (P < 0.05) between three land management systems. The abundance of seedlings, saplings and young shrubs were significantly greater (P < 0.05) in the ranch than the game reserve and communal area. The mature trees had a significantly greater density (P < 0.05) in the game reserve than the ranch and communal area. Soil analysis results showed that Magnesium (Mg), Potassium (K), Manganese (Mn) and Copper (Cu) had similar (P > 0.05) concentrations in soils obtained from all land management systems in both seasons. Soil Calcium (Ca) levels showed differences between land management systems in winter only being greater (P < 0.001) in the game reserve than the ranch and the communal lands. Soil Phosphorus (P) and Iron (Fe) also showed variations between land management systems in winter being significantly greater (P < 0.001) in the ranch than the other land management systems. The concentration of soil K differed between the two seasons in soils collected from the ranch and the communal lands, being greater (P < 0.001) in winter than summer seasons. Soil Zinc (Zn) also showed differences between land management systems in summer being greater (P < 0.001) in the game reserve than in the ranch and communal land. Seasonal variations were observed in soil P level of samples collected from the ranch being greater (P < 0.001) in winter than summer season. The concentration of soil Zn was higher (P < 0.001) in the soil collected from the game reserve and the ranch in summer than winter season. Woody forage analysis results showed that browse samples harvested from the ranch had greater (P < 0.001) Nitrogen (N) level than the other land management systems in both seasons. The browse samples harvested from the game reserve had greater (P < 0.001) Ca level than the other land management systems in winter season only. The level of P showed variations (P < 0.05) between land management systems in samples harvested during winter only being greater (P < 0.001) in samples from the ranch than the other land management systems. Similarly, browse harvested during winter showed significant differences (P < 0.05) in K levels being lowest in samples collected from game reserves, where the Ca level was greatest. Browse samples collected from the communal land and the ranch had the lowest Mg levels in summer and winter seasons, respectively. In all management systems, browse harvested during summer had greater (P < 0.001) N and P levels than those harvested in winter, and the reverse holds true for K and Ca levels. Browse samples harvested during winter had greater (P < 0.001) Mn levels in samples from the communal area and game reserve than the ranch, but in summer, the game reserve had higher Mn level than the other two sites. Browse samples harvested during winter only showed differences (P < 0.05) in Cu and Zn levels being greatest (P < 0.001) in samples collected from ranch and game reserves. In all management systems, browse samples harvested during winter had greater (P < 0.001) Fe, Mn, Zn and Cu (except for the game reserve and communal land) levels than those harvested in summer. Herbaceous analysis results showed that samples harvested in summer from the game reserve and ranch had greater (P < 0.001) TOC levels than the communal area, but in winter, samples from the game reserve and ranch had similar TOC levels, except for communal areas that had no herbaceous vegetation. Herbaceous samples harvested in winter from the ranch had greater (P < 0.001) TN levels than the game reserve, and that made the C:N ratio higher (P < 0.001) in the game reserve. Woody foliar samples harvested in winter from the game reserve and communal lands had greater (P < 0.001) TOC than the ranch. Values on C:N ratio showed that in winter, the communal and ranch had greater (P < 0.001) C:N than the game reserve, but in summer, the ranch had higher (P < 0.001) C:N than the game reserve and communal area. The vegetation TOC (kg ha-1) showed the order of the game reserve > ranch > communal area and the trend was similar in both seasons. In summer, the game reserve had the greatest (P < 0.001) TN (kg ha-1) followed by the ranch and the lowest in the communal area, but in winter the game reserve and ranch had similar and higher (P < 0.001) TN (kg ha-1) than the communal areas. In summer, both the game reserve and ranch recorded greater (P < 0.001) herbaceous δ13C and lowest δ15N than the communal area, but in winter, the game reserve had higher (P < 0.001) δ13C than the ranch, though both the game reserve and ranch had similar (P > 0.001) δ15N values. In summer, both the communal and ranch had slightly higher (P < 0.001) woody foliar δ13C than the game reserve, but in winter the ranch had greater (P < 0.001) δ13C than game reserve and communal areas. Woody foliar samples harvested from the ranch showed variations (P < 0.05) in δ15N between seasons being higher (P < 0.001) in summer than winter. The TOC was greatest in summer, in soils collected from the game reserve followed by the ranch and least in the communal land, but in winter, the lowest TOC was recorded in soils collected from the ranch. The soil C:N ratio varied between land management systems in soils collected during summer only being greater (P < 0.001) in the game and ranch than the communal area. The soil TOC (kg ha-1) and TN (kg ha-1) did not show (P > 0.05) differences between land management systems. Soil δ13C level was remarkably (P < 0.05) affected by land management systems, but did not interact with seasons. In both seasons, the communal land had slightly higher (P < 0.001) δ13C level than the other two land management systems. In both seasons, δ15N levels showed great differences (P < 0.001) between land management systems, with highest soil δ15N levels observed in the communal area than the ranch and game reserve. Results on social survey showed that the majority of the interviewed households in Omaandi (63percent), Oshitutuma (53percent) and Epukunoyana (50percent) were female headed families and the remaining were male headed. The mean population of goats and cattle were significantly higher (P < 0.05) in Omaandi and Epukunoyana than in Oshitutuma village, respectively. In this study, household respondents in all villages indicated that all livestock species were primarily kept for wealth status and provision of meat for consumption. Respondents in all villages also indicated that lack of grazing lands and shortage of feed were the primary constraints of livestock production followed by water scarcity and recurring droughts. Elder respondents in all villages perceived overgrazing and climate change as the primary cause of rangeland degradation. Elder interviewees in Omaandi and Oshitutuma villages regarded low grass cover and dominance of poor quality grass (Epukunoyana only) as the primary vegetation indicators of rangeland degradation. The animal related indicators such as stunted animal body as a result of mineral deficiency, high livestock mortality, emaciated body and animals traveling long distance for grazing and water (Oshitutuma only) were regarded in all villages as the primary livestock related indicators of rangeland degradation. Elders from Epukunoyana village ranked low rainfall, high soil and atmospheric temperatures as the primary climate related indicators of rangeland degradation, although in Omaandi and Oshitutuma, high soil and atmospheric temperatures were considered as the primary climate related indicators of rangeland degradation. Based on the findings, this study concludes that land management systems had great influences on the distribution and production of herbaceous and woody vegetation in summer and winter seasons. This study also observed that land management systems interacted strongly with season to influence most vegetation and soil variables. Lastly, based on the study findings, the study concludes that all three communal rangelands are degraded and degradation led to a gradual declining trend in the livestock population over the past 10 to 20 years in the communal areas as a result of feed shortage.
- Full Text:
- Date Issued: 2018
- Authors: Kahumba, Absalom
- Date: 2018
- Subjects: Rural development Land use Land tenure
- Language: English
- Type: Thesis , Doctoral , Pasture Science
- Identifier: http://hdl.handle.net/10353/11718 , vital:39100
- Description: This study was conducted in Ogongo district, situated in Omusati Region of the central northern Namibia. The objectives of this study were to assess the impact of land use practices on 1) vegetation distribution and status; 2) soil properties; 3) carbon-nitrogen sequestration potential, and 4) investigate the indigenous pastoralists’ knowledge and perceptions on livestock production, rangeland degradation and its indicators in three villages (Omaandi, Epukunoyana and Oshitutuma) in Mopane savannah rangelands of central northern Namibia. For vegetation and soil assessment, three grazing land management systems (communal, game reserve and cattle ranch) were identified adjacent to each other. In each grazing system, three sites (for game reserve and cattle ranch, these were camps) were selected. In each site, three belt transects of 50 m x 5 m2 were marked to record vegetation and soil data as well as to collect vegetation samples for analysis. Grass species composition was recorded using step point methods from 250 points per belt transect. A total of five 0.25 m2 quadrats were randomly laid within each 250 m2 belt transect to record the herbaceous plant density, tuft diameter, tuft distance and biomass. All woody plants in the belt transect (250 m2) were measured and recorded for species plant height and canopy diameters. Three soil samples per belt transect were collected at the time of forage sampling down to a depth of 20 cm using a soil auger. Soil and plant samples were analysed for macro and micro minerals, total organic carbon, total nitrogen, carbon-nitrogen ratios, and carbon and nitrogen isotopes. For bulk density, soil samples were collected using soil core samplers. In the social study, a total of 90 households were randomly selected from three villages and a total of 48 elders were purposefully selected based on the age, time spent in the village and livestock ownership. A combination of structured and semi-structured questionnaires were used to gather household information, while a structured questionnaire and open ended discussion were used to interview elders on the constraints of livestock production, rangeland degradation related issues and their consequences on livelihood. Field vegetation and soil data were analyzed using a General Linear Model (GLM) in SAS (2007), whereas the social data were analyzed using SPSS version 21 (2014). A total of 23 grass species were identified in all three land management systems, of which 48% were perennials and the remaining 52% were annuals. Aristida meridionalis dominated the game reserve and the ranch, whereas Eragrostis trichophora was dominant in all land management systems. Forbs occurred commonly in the game reserve and the ranch, but were dominant in the communal areas. The abundance of A. meridionalis responded significantly (P < 0.001) to land management systems with the game reserve showing remarkably greater abundance than the ranch. In summer, the abundance of E. trichophora was high (P < 0.001) in the game reserve followed by the communal and ranch sites, whereas in winter, the species had still greater abundance in the game reserve followed by the ranch. In summer, the mean grass density was greatest (P < 0.001) in the game reserve and least in the communal lands, but vice versa for the tuft distance. In summer, the average distance between tufts showed great variations (P < 0.001) with the communal areas having greater values than the ranch and game reserve. In winter, both the game reserve and the ranch showed records of similar grass tuft density and distance between tufts, whereas these values were nil for communal areas, because, there were no grasses recorded. Herbaceous plant dry matter was significantly greater (P < 0.05) in the game reserve followed by the ranch and the least in the communal areas. Results on biomass showed remarkably higher values (P < 0.001) in summer than winter in all land management systems. The total woody plant density was significantly higher (P < 0.001) in the ranch (542 TE ha-1) than the game reserve (449 TE ha-1) and communal area (324 TE ha-1). However, percentage of canopy cover did not differ significantly (P > 0.05) between the three land management systems. Woody plant density of height classes were significantly different (P < 0.05) between three land management systems. The abundance of seedlings, saplings and young shrubs were significantly greater (P < 0.05) in the ranch than the game reserve and communal area. The mature trees had a significantly greater density (P < 0.05) in the game reserve than the ranch and communal area. Soil analysis results showed that Magnesium (Mg), Potassium (K), Manganese (Mn) and Copper (Cu) had similar (P > 0.05) concentrations in soils obtained from all land management systems in both seasons. Soil Calcium (Ca) levels showed differences between land management systems in winter only being greater (P < 0.001) in the game reserve than the ranch and the communal lands. Soil Phosphorus (P) and Iron (Fe) also showed variations between land management systems in winter being significantly greater (P < 0.001) in the ranch than the other land management systems. The concentration of soil K differed between the two seasons in soils collected from the ranch and the communal lands, being greater (P < 0.001) in winter than summer seasons. Soil Zinc (Zn) also showed differences between land management systems in summer being greater (P < 0.001) in the game reserve than in the ranch and communal land. Seasonal variations were observed in soil P level of samples collected from the ranch being greater (P < 0.001) in winter than summer season. The concentration of soil Zn was higher (P < 0.001) in the soil collected from the game reserve and the ranch in summer than winter season. Woody forage analysis results showed that browse samples harvested from the ranch had greater (P < 0.001) Nitrogen (N) level than the other land management systems in both seasons. The browse samples harvested from the game reserve had greater (P < 0.001) Ca level than the other land management systems in winter season only. The level of P showed variations (P < 0.05) between land management systems in samples harvested during winter only being greater (P < 0.001) in samples from the ranch than the other land management systems. Similarly, browse harvested during winter showed significant differences (P < 0.05) in K levels being lowest in samples collected from game reserves, where the Ca level was greatest. Browse samples collected from the communal land and the ranch had the lowest Mg levels in summer and winter seasons, respectively. In all management systems, browse harvested during summer had greater (P < 0.001) N and P levels than those harvested in winter, and the reverse holds true for K and Ca levels. Browse samples harvested during winter had greater (P < 0.001) Mn levels in samples from the communal area and game reserve than the ranch, but in summer, the game reserve had higher Mn level than the other two sites. Browse samples harvested during winter only showed differences (P < 0.05) in Cu and Zn levels being greatest (P < 0.001) in samples collected from ranch and game reserves. In all management systems, browse samples harvested during winter had greater (P < 0.001) Fe, Mn, Zn and Cu (except for the game reserve and communal land) levels than those harvested in summer. Herbaceous analysis results showed that samples harvested in summer from the game reserve and ranch had greater (P < 0.001) TOC levels than the communal area, but in winter, samples from the game reserve and ranch had similar TOC levels, except for communal areas that had no herbaceous vegetation. Herbaceous samples harvested in winter from the ranch had greater (P < 0.001) TN levels than the game reserve, and that made the C:N ratio higher (P < 0.001) in the game reserve. Woody foliar samples harvested in winter from the game reserve and communal lands had greater (P < 0.001) TOC than the ranch. Values on C:N ratio showed that in winter, the communal and ranch had greater (P < 0.001) C:N than the game reserve, but in summer, the ranch had higher (P < 0.001) C:N than the game reserve and communal area. The vegetation TOC (kg ha-1) showed the order of the game reserve > ranch > communal area and the trend was similar in both seasons. In summer, the game reserve had the greatest (P < 0.001) TN (kg ha-1) followed by the ranch and the lowest in the communal area, but in winter the game reserve and ranch had similar and higher (P < 0.001) TN (kg ha-1) than the communal areas. In summer, both the game reserve and ranch recorded greater (P < 0.001) herbaceous δ13C and lowest δ15N than the communal area, but in winter, the game reserve had higher (P < 0.001) δ13C than the ranch, though both the game reserve and ranch had similar (P > 0.001) δ15N values. In summer, both the communal and ranch had slightly higher (P < 0.001) woody foliar δ13C than the game reserve, but in winter the ranch had greater (P < 0.001) δ13C than game reserve and communal areas. Woody foliar samples harvested from the ranch showed variations (P < 0.05) in δ15N between seasons being higher (P < 0.001) in summer than winter. The TOC was greatest in summer, in soils collected from the game reserve followed by the ranch and least in the communal land, but in winter, the lowest TOC was recorded in soils collected from the ranch. The soil C:N ratio varied between land management systems in soils collected during summer only being greater (P < 0.001) in the game and ranch than the communal area. The soil TOC (kg ha-1) and TN (kg ha-1) did not show (P > 0.05) differences between land management systems. Soil δ13C level was remarkably (P < 0.05) affected by land management systems, but did not interact with seasons. In both seasons, the communal land had slightly higher (P < 0.001) δ13C level than the other two land management systems. In both seasons, δ15N levels showed great differences (P < 0.001) between land management systems, with highest soil δ15N levels observed in the communal area than the ranch and game reserve. Results on social survey showed that the majority of the interviewed households in Omaandi (63percent), Oshitutuma (53percent) and Epukunoyana (50percent) were female headed families and the remaining were male headed. The mean population of goats and cattle were significantly higher (P < 0.05) in Omaandi and Epukunoyana than in Oshitutuma village, respectively. In this study, household respondents in all villages indicated that all livestock species were primarily kept for wealth status and provision of meat for consumption. Respondents in all villages also indicated that lack of grazing lands and shortage of feed were the primary constraints of livestock production followed by water scarcity and recurring droughts. Elder respondents in all villages perceived overgrazing and climate change as the primary cause of rangeland degradation. Elder interviewees in Omaandi and Oshitutuma villages regarded low grass cover and dominance of poor quality grass (Epukunoyana only) as the primary vegetation indicators of rangeland degradation. The animal related indicators such as stunted animal body as a result of mineral deficiency, high livestock mortality, emaciated body and animals traveling long distance for grazing and water (Oshitutuma only) were regarded in all villages as the primary livestock related indicators of rangeland degradation. Elders from Epukunoyana village ranked low rainfall, high soil and atmospheric temperatures as the primary climate related indicators of rangeland degradation, although in Omaandi and Oshitutuma, high soil and atmospheric temperatures were considered as the primary climate related indicators of rangeland degradation. Based on the findings, this study concludes that land management systems had great influences on the distribution and production of herbaceous and woody vegetation in summer and winter seasons. This study also observed that land management systems interacted strongly with season to influence most vegetation and soil variables. Lastly, based on the study findings, the study concludes that all three communal rangelands are degraded and degradation led to a gradual declining trend in the livestock population over the past 10 to 20 years in the communal areas as a result of feed shortage.
- Full Text:
- Date Issued: 2018
Bacterial colonisation and degradation of geologically weathered and discard coal
- Authors: Olawale, Jacob Taiwo
- Date: 2018
- Subjects: Coal mine waste , Coal -- Biodegradation , Coal mines and mining -- Environmental aspects , Land degradation , Electron microscopy , Extracellular polymeric substances , Flagella (Microbiology) , Fourier transform infrared spectroscopy , Microbiologically influenced corrosion
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61625 , vital:28043
- Description: Bacterial beneficiation of low-grade coal, coal discard, and waste has the potential to mitigate land degradation, water and soil pollution and, be a strategy for mining companies to responsibly extract and process coal with environmental sustainability. This study investigated the colonisation and biodegradation or depolymerisation of coal discard and geologically weathered coal by selected strains of bacteria, and an attempt has been made to describe the mechanisms associated with colonisation and biodegradation of this carbonaceous material. Ten bacterial strains, Bacillus strain ECCN 18b, Citrobacter strain ECCN 19b, Proteus strain ECCN 20b, Exiguobacterium strain ECCN 21b, Microbacterium strain ECCN 22b, Proteus strain ECCN 23b, Serratia strain ECCN 24b, Escherichia strain ECCN 25b, Bacillus strain ECCN 26b and Bacillus strain ECCN 41b, isolated from diesel-contaminated soil and coal slurry and identified using DNA sequencing, were rescreened and their coal biodegradation potential ranked. The ranking of the bacterial strains was undertaken using several indicators including; formation of brown halos on the plate culture (solid), change in colour intensity of the medium in liquid culture, change in culture media pH, and an increase in absorbance at 280nm and 450nm. Although, all the ten strains showed evidence of biodegradation of coal discard and geologically weathered coal based on the ranking employed, and the three strains considered the best candidates were Citrobacter strain ECCN 19b, Exiguobacterium strain ECCN 21b and Serratia strain ECCN 24b. The actions of the three bacterial strains were further studied and characterised in relation to coal degradation. Electron microscopy revealed that Citrobacter strain ECCN 19b, Exiguobacterium strain ECCN 21b and Serratia strain ECCN 24b attached to the surface of coal discard and geologically weathered coal by a process that appeared to involve extracellular polymeric substances (EPS), and flagella. The presence of flagella for Citrobacter strain ECCN 19b and Serratia strain ECCN 24b was confirmed by transmission electron microscopy. Bacterial degradation of coal discard and geologically weathered coal by these selected strains resulted in the release of soluble and insoluble products. Ultraviolet/ visible spectrophotometric (UV/VIS) analysis revealed that the soluble products resembled humic acid-like substances, which was confirmed following Fourier Transform Infrared (FTIR) spectroscopy. Analysis revealed that the coal-derived humic acid-like substances were similar to commercial humic acid extracted from bituminous coal. Elemental analysis of the insoluble product residue after bacterial biodegradation revealed the modification of the chemical compositions of the coal discard and geologically weathered coal substrates. Characterisation of the functional groups of the insoluble product using FTIR spectroscopy indicated changes, with the appearance of new peaks at 1737cm-1, 1366cm-1, 1228cm-1, and 1216cm-1 characteristic of aldehyde, ketones, carboxylic acids, esters, amines, and alkanes. Broad spectra regions of 3500 -3200cm-1, characteristic of alcohol and phenol, were also observed. Together, these results were taken as evidence for increased oxidation of the coal substrates, presumably as a consequence of bacterial catalysed biodegradation of coal discard and geologically weathered coal. During bacterial degradation of coal discard and geologically weathered coal, strains produced extracellular protein, which was detected and further investigated using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS- PAGE). At least three protein bands with molecular mass 53 kDa, 72 kDa, and 82 kDa were common to the three bacterial strains. Following ammonium sulphate precipitation and gel filtration chromatography, additional bands with molecular mass 16 kDa, 33 kDa, 37 kDa, and 43 kDa were detected. An extracellular laccase activity was detected in cultures of Exiguobacterium strain ECCN 21b and Serratia strain ECCN 24b. Cytochrome P450 activity was detected in all the bacterial strains in the presence of both coal discard and geologically weathered coal. This is the first time that cytochrome P450 activity has been reported following exposure of these three bacterial strains to a coal substrate. Overall, this research has successfully demonstrated the partial degradation of coal discard and geologically weathered coal by Citrobacter strain ECCN 19b, Exiguobacterium strain ECCN 21b and Serratia strain ECCN 24b and the release of humic acid-like substances. Thus, the biodegradation process involved adherence to and growth of the bacteria on the surface of coal substrate and appeared to require the formation of alkaline substances and the combined activities of extracellular LAC and cytochrome P450. Since bacterial degradation of low-grade coal and discard appears to be viable, the bacteria isolated in this study can potentially be used either for conversion of discard into valuable chemicals or to mitigate the deleterious effects of stockpiled coal discard on the environment.
- Full Text:
- Date Issued: 2018
- Authors: Olawale, Jacob Taiwo
- Date: 2018
- Subjects: Coal mine waste , Coal -- Biodegradation , Coal mines and mining -- Environmental aspects , Land degradation , Electron microscopy , Extracellular polymeric substances , Flagella (Microbiology) , Fourier transform infrared spectroscopy , Microbiologically influenced corrosion
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61625 , vital:28043
- Description: Bacterial beneficiation of low-grade coal, coal discard, and waste has the potential to mitigate land degradation, water and soil pollution and, be a strategy for mining companies to responsibly extract and process coal with environmental sustainability. This study investigated the colonisation and biodegradation or depolymerisation of coal discard and geologically weathered coal by selected strains of bacteria, and an attempt has been made to describe the mechanisms associated with colonisation and biodegradation of this carbonaceous material. Ten bacterial strains, Bacillus strain ECCN 18b, Citrobacter strain ECCN 19b, Proteus strain ECCN 20b, Exiguobacterium strain ECCN 21b, Microbacterium strain ECCN 22b, Proteus strain ECCN 23b, Serratia strain ECCN 24b, Escherichia strain ECCN 25b, Bacillus strain ECCN 26b and Bacillus strain ECCN 41b, isolated from diesel-contaminated soil and coal slurry and identified using DNA sequencing, were rescreened and their coal biodegradation potential ranked. The ranking of the bacterial strains was undertaken using several indicators including; formation of brown halos on the plate culture (solid), change in colour intensity of the medium in liquid culture, change in culture media pH, and an increase in absorbance at 280nm and 450nm. Although, all the ten strains showed evidence of biodegradation of coal discard and geologically weathered coal based on the ranking employed, and the three strains considered the best candidates were Citrobacter strain ECCN 19b, Exiguobacterium strain ECCN 21b and Serratia strain ECCN 24b. The actions of the three bacterial strains were further studied and characterised in relation to coal degradation. Electron microscopy revealed that Citrobacter strain ECCN 19b, Exiguobacterium strain ECCN 21b and Serratia strain ECCN 24b attached to the surface of coal discard and geologically weathered coal by a process that appeared to involve extracellular polymeric substances (EPS), and flagella. The presence of flagella for Citrobacter strain ECCN 19b and Serratia strain ECCN 24b was confirmed by transmission electron microscopy. Bacterial degradation of coal discard and geologically weathered coal by these selected strains resulted in the release of soluble and insoluble products. Ultraviolet/ visible spectrophotometric (UV/VIS) analysis revealed that the soluble products resembled humic acid-like substances, which was confirmed following Fourier Transform Infrared (FTIR) spectroscopy. Analysis revealed that the coal-derived humic acid-like substances were similar to commercial humic acid extracted from bituminous coal. Elemental analysis of the insoluble product residue after bacterial biodegradation revealed the modification of the chemical compositions of the coal discard and geologically weathered coal substrates. Characterisation of the functional groups of the insoluble product using FTIR spectroscopy indicated changes, with the appearance of new peaks at 1737cm-1, 1366cm-1, 1228cm-1, and 1216cm-1 characteristic of aldehyde, ketones, carboxylic acids, esters, amines, and alkanes. Broad spectra regions of 3500 -3200cm-1, characteristic of alcohol and phenol, were also observed. Together, these results were taken as evidence for increased oxidation of the coal substrates, presumably as a consequence of bacterial catalysed biodegradation of coal discard and geologically weathered coal. During bacterial degradation of coal discard and geologically weathered coal, strains produced extracellular protein, which was detected and further investigated using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS- PAGE). At least three protein bands with molecular mass 53 kDa, 72 kDa, and 82 kDa were common to the three bacterial strains. Following ammonium sulphate precipitation and gel filtration chromatography, additional bands with molecular mass 16 kDa, 33 kDa, 37 kDa, and 43 kDa were detected. An extracellular laccase activity was detected in cultures of Exiguobacterium strain ECCN 21b and Serratia strain ECCN 24b. Cytochrome P450 activity was detected in all the bacterial strains in the presence of both coal discard and geologically weathered coal. This is the first time that cytochrome P450 activity has been reported following exposure of these three bacterial strains to a coal substrate. Overall, this research has successfully demonstrated the partial degradation of coal discard and geologically weathered coal by Citrobacter strain ECCN 19b, Exiguobacterium strain ECCN 21b and Serratia strain ECCN 24b and the release of humic acid-like substances. Thus, the biodegradation process involved adherence to and growth of the bacteria on the surface of coal substrate and appeared to require the formation of alkaline substances and the combined activities of extracellular LAC and cytochrome P450. Since bacterial degradation of low-grade coal and discard appears to be viable, the bacteria isolated in this study can potentially be used either for conversion of discard into valuable chemicals or to mitigate the deleterious effects of stockpiled coal discard on the environment.
- Full Text:
- Date Issued: 2018
Baculovirus synergism: investigating mixed alphabaculovirus and betabaculovirus infections in the false codling moth, thaumatotibia leucotreta, for improved pest control
- Authors: Jukes, Michael David
- Date: 2018
- Subjects: Baculoviruses , Cryptophlebia leucotreta -- Biological control , Citrus -- Diseases and pests -- South Africa , Pests -- Integrated control , Nucleopolyhedroviruses , Natural pesticides , Cryptophlebia leucotreta granulovirus (CrleGV)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61797 , vital:28061
- Description: Baculovirus based biopesticides are an effective and environmentally friendly approach for the control of agriculturally important insect pests. The false codling moth (FCM), Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae), is indigenous to southern Africa and is a major pest of citrus crops. This moth poses a serious risk to export of fruit to foreign markets and the control of this pest is therefore imperative. The Cryptophlebia leucotreta granulovirus (CrleGV) has been commercially formulated into the products Cryptogran™ and Cryptex®. These products have been used successfully for over a decade as part of a rigorous integrated pest management (IPM) programme to control T. leucotreta in South Africa. There is however, a continuous need to improve this programme while also addressing new challenges as they arise. An example of a rising concern is the possibility of resistance developing towards CrleGV. This was seen in Europe with field populations of the codling moth, Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae), which developed resistance to the Mexican isolate of the Cydia pomonella granulovirus (CpGV-M). To prevent such a scenario occurring in South Africa, there is a need to improve existing methods of control. For example, additional baculovirus variants can be isolated and characterised for determining virulence, which can then be developed as new biopesticides. Additionally, the potential for synergistic effects between different baculoviruses infecting the same host can be explored for improved virulence. A novel nucleopolyhedrovirus was recently identified in T. leucotreta larval homogenates which were also infected with CrleGV. This provided unique opportunities for continued research and development. In this study, a method using C. pomonella larvae, which can be infected by the NPV but not by CrleGV, was developed to separate the NPV from GV-NPV mixtures in an in vivo system. Examination of NPV OBs by transmission electron microscopy showed purified occlusion bodies with a single nucleopolyhedrovirus morphology (SNPV). Genetic characterisation identified the novel NPV as Cryptophlebia peltastica nucleopolyhedrovirus (CrpeNPV), which was recently isolated from the litchi moth, Cryptophlebia peltastica (Meyrick) (Lepidoptera: Tortricidae). To begin examining the potential for synergism between the two viruses, a multiplex PCR assay was developed to accurately detect CrleGV and/or CrpeNPV in mixed infections. This assay was applied to various samples to screen for the presence of CrpeNPV and CrleGV. Additionally, a validation experiment was performed using different combinations of CrpeNPV and/or CrleGV to evaluate the effectiveness of the mPCR assay. The results obtained indicated a high degree of specificity with the correct amplicons generated for each test sample. The biological activity of CrpeNPV and CrleGV were evaluated using surface dose bioassays, both individually and in various combinations, against T. leucotreta neonate larvae in a laboratory setting. A synergistic effect was recorded in the combination treatments, showing improved virulence when compared against each virus in isolation. The LC90 for CrpeNPV and CrleGV when applied alone against T. leucotreta was calculated to be 2.75*106 and 3.00*106 OBs.ml"1 respectively. These values decreased to 1.07*106 and 7.18*105 OBs.ml"1 when combinations of CrleGV and CrpeNPV were applied at ratios of 3:1 and 1:3 respectively. These results indicate a potential for developing improved biopesticides for the control of T. leucotreta in the field. To better understand the interactions between CrleGV and CrpeNPV, experiments involving the serial passage of these viruses through T. leucotreta larvae were performed. This was done using each virus in isolation as well as both viruses in different combinations. Genomic DNA was extracted from recovered occlusion bodies after each passage and examined by multiplex and quantitative PCR. This analysis enabled the detection of each virus present throughout this assay, as well as recording shifts in the ratio of CrleGV and CrpeNPV at each passage. CrleGV rapidly became the dominant virus in all treatments, indicating a potentially antagonistic interaction during serial passage. Additionally, CrpeNPV and CrleGV were detected in treatments which were not originally inoculated with one or either virus, indicating potential covert infections in T. leucotreta. Occlusion bodies recovered from the final passage were used to inoculate C. pomonella larvae to isolate CrpeNPV from CrleGV. Genomic DNA was extracted from these CrpeNPV OBs and examined by restriction endonuclease assays and next generation sequencing. This enabled the identification of potential recombination events which may have occurred during the dual GV and NPV infections throughout the passage assay. No recombination events were identified in the CrpeNPV genome sequences assembled from virus collected at the end of the passage assay. Lastly, the efficacy of CrpeNPV and CrleGV, both alone and in various combinations, was evaluated in the field. In two separate trials conducted on citrus, unfavorable field conditions resulted in no significant reduction in fruit infestation for both the virus and chemical treatments. While not statistically significant, virus treatments were recorded to have the lowest levels of fruit infestation with a measured reduction of up to 64 %. This study is the first to report a synergistic effect between CrleGV and CrpeNPV in T. leucotreta. The discovery of beneficial interactions creates an opportunity for the development of novel biopesticides for improved control of this pest in South Africa.
- Full Text:
- Date Issued: 2018
- Authors: Jukes, Michael David
- Date: 2018
- Subjects: Baculoviruses , Cryptophlebia leucotreta -- Biological control , Citrus -- Diseases and pests -- South Africa , Pests -- Integrated control , Nucleopolyhedroviruses , Natural pesticides , Cryptophlebia leucotreta granulovirus (CrleGV)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61797 , vital:28061
- Description: Baculovirus based biopesticides are an effective and environmentally friendly approach for the control of agriculturally important insect pests. The false codling moth (FCM), Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae), is indigenous to southern Africa and is a major pest of citrus crops. This moth poses a serious risk to export of fruit to foreign markets and the control of this pest is therefore imperative. The Cryptophlebia leucotreta granulovirus (CrleGV) has been commercially formulated into the products Cryptogran™ and Cryptex®. These products have been used successfully for over a decade as part of a rigorous integrated pest management (IPM) programme to control T. leucotreta in South Africa. There is however, a continuous need to improve this programme while also addressing new challenges as they arise. An example of a rising concern is the possibility of resistance developing towards CrleGV. This was seen in Europe with field populations of the codling moth, Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae), which developed resistance to the Mexican isolate of the Cydia pomonella granulovirus (CpGV-M). To prevent such a scenario occurring in South Africa, there is a need to improve existing methods of control. For example, additional baculovirus variants can be isolated and characterised for determining virulence, which can then be developed as new biopesticides. Additionally, the potential for synergistic effects between different baculoviruses infecting the same host can be explored for improved virulence. A novel nucleopolyhedrovirus was recently identified in T. leucotreta larval homogenates which were also infected with CrleGV. This provided unique opportunities for continued research and development. In this study, a method using C. pomonella larvae, which can be infected by the NPV but not by CrleGV, was developed to separate the NPV from GV-NPV mixtures in an in vivo system. Examination of NPV OBs by transmission electron microscopy showed purified occlusion bodies with a single nucleopolyhedrovirus morphology (SNPV). Genetic characterisation identified the novel NPV as Cryptophlebia peltastica nucleopolyhedrovirus (CrpeNPV), which was recently isolated from the litchi moth, Cryptophlebia peltastica (Meyrick) (Lepidoptera: Tortricidae). To begin examining the potential for synergism between the two viruses, a multiplex PCR assay was developed to accurately detect CrleGV and/or CrpeNPV in mixed infections. This assay was applied to various samples to screen for the presence of CrpeNPV and CrleGV. Additionally, a validation experiment was performed using different combinations of CrpeNPV and/or CrleGV to evaluate the effectiveness of the mPCR assay. The results obtained indicated a high degree of specificity with the correct amplicons generated for each test sample. The biological activity of CrpeNPV and CrleGV were evaluated using surface dose bioassays, both individually and in various combinations, against T. leucotreta neonate larvae in a laboratory setting. A synergistic effect was recorded in the combination treatments, showing improved virulence when compared against each virus in isolation. The LC90 for CrpeNPV and CrleGV when applied alone against T. leucotreta was calculated to be 2.75*106 and 3.00*106 OBs.ml"1 respectively. These values decreased to 1.07*106 and 7.18*105 OBs.ml"1 when combinations of CrleGV and CrpeNPV were applied at ratios of 3:1 and 1:3 respectively. These results indicate a potential for developing improved biopesticides for the control of T. leucotreta in the field. To better understand the interactions between CrleGV and CrpeNPV, experiments involving the serial passage of these viruses through T. leucotreta larvae were performed. This was done using each virus in isolation as well as both viruses in different combinations. Genomic DNA was extracted from recovered occlusion bodies after each passage and examined by multiplex and quantitative PCR. This analysis enabled the detection of each virus present throughout this assay, as well as recording shifts in the ratio of CrleGV and CrpeNPV at each passage. CrleGV rapidly became the dominant virus in all treatments, indicating a potentially antagonistic interaction during serial passage. Additionally, CrpeNPV and CrleGV were detected in treatments which were not originally inoculated with one or either virus, indicating potential covert infections in T. leucotreta. Occlusion bodies recovered from the final passage were used to inoculate C. pomonella larvae to isolate CrpeNPV from CrleGV. Genomic DNA was extracted from these CrpeNPV OBs and examined by restriction endonuclease assays and next generation sequencing. This enabled the identification of potential recombination events which may have occurred during the dual GV and NPV infections throughout the passage assay. No recombination events were identified in the CrpeNPV genome sequences assembled from virus collected at the end of the passage assay. Lastly, the efficacy of CrpeNPV and CrleGV, both alone and in various combinations, was evaluated in the field. In two separate trials conducted on citrus, unfavorable field conditions resulted in no significant reduction in fruit infestation for both the virus and chemical treatments. While not statistically significant, virus treatments were recorded to have the lowest levels of fruit infestation with a measured reduction of up to 64 %. This study is the first to report a synergistic effect between CrleGV and CrpeNPV in T. leucotreta. The discovery of beneficial interactions creates an opportunity for the development of novel biopesticides for improved control of this pest in South Africa.
- Full Text:
- Date Issued: 2018
Bayesian spatial modelling of tuberculosis and its effects on socio-economic and demographic factors in South Africa : a case study of the Eastern Cape Province
- Authors: Obaromi, Abiodun Davies
- Date: 2018
- Subjects: Tuberculosis -- South Africa -- Eastern Cape Tuberculosis -- Epidemiology -- Statistics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9648 , vital:34813
- Description: This dissertation is concerned with evolving and extending statistical models in the area of Bayesian spatial modelling, an increasingly important field of spatial epidemiology with particular interest towards application to Tuberculosis data in the Eastern Cape province of South Africa. In spatial epidemiology, the diseases to be examined usually occur within a map that needs spatial statistical methods that are appropriate, to model the observed data in the presence of some covariates and also cater for the variation of the disease. In this thesis, the Bayesian models were developed in such a way that they allowed several factors classified as fixed and random effects, to be included in the models and using the Bayesian approach. The basic model used in disease mapping is the Besag, York and Mollie model, which incorporates two random effects; one which is spatially structured and the other random effect which is spatially unstructured. The effects (fixed and random) were the covariate effects, socio-economic and demographic variability and the spatial variability respectively, which were all investigated in seven different hierarchical/multilevel Bayesian models. These factors showed varying and substantial effects in the posterior relative risk estimation of the disease. We assumed a negative binomial and generalized Poisson distributions to the response variable or relative risk estimate, 𝑦𝑖 ,to capture the over-dispersion phenomenon that is common and inherent with Poisson density for counts data. Spatial and non-spatial models were developed to model over-dispersion with all the distributions; Poisson, negative binomial and generalized Poisson. Negative binomial and generalized Poisson showed varying properties from comparisons with DIC values and parameter estimates to standard errors, which made either of them fit depending on the choice of model selection. It was found that a lower DIC value could be insufficient to determine a best fit model, if other models present estimates with lower variances even at higher DIC values. The generalized Poisson, a two parameter distribution like the negative binomial, which also has the ability to capture both under-dispersion and over-dispersion, was found to perform better in the results than the negative binomial on the basis of a lower variance and with more exact parameter estimates. A new weighted prior distribution, the “Besag2” ICAR model for the structured spatial random effects, which is an extension of the traditional ICAR prior model with two hyperparameters, was also developed and compared with some existing prior models; BYM and ICAR, to measure for spatial dependency in the regions. This new prior distribution was found to show a better fit, when compared to the basic ICAR prior usually assumed for the spatial random effect in the BYM model. This newly parameterized prior distribution in the Besag, York and Mollie model also led to improved parameter control, as the hyperparameters can be seen independently from each other. The result also showed that the new model performed well, both presenting good learning abilities and good shrinkage behaviour. In terms of model choice criteria, the proposed model performed at least equally well and better than the existing models, and the new formulation also gave parameters that are interpretable and have a clearer meaning. To interpolate scattered or regularly distributed data, there are imprecise or exact methods, but there are some of these methods that could be used for interpolating data in a regular grid and others in an irregular grid. Linear and biharmonic spline methods were implemented in MATLAB, to compare for smoothing in the distribution patterns of tuberculosis in the province. This smoothing spline is a method of fitting a smooth curve to a set of noisy observations using a spline function. This new method is rarely used in disease mapping applications, but it has a superior advantage to be assessed at subjective locations rather than only on a rectangular grid as seen in most traditional GIS methods of geospatial analyses. The proposed new models and methods in this thesis were found to be flexible and robust, since they can be reduced or extended according to the nature of the data. Nevertheless, great care must be considered in the choice of prior densities. The approaches developed in this dissertation helped to broaden the scope for spatial analysis and disease mapping applications in epidemiology and public health studies.
- Full Text:
- Date Issued: 2018
- Authors: Obaromi, Abiodun Davies
- Date: 2018
- Subjects: Tuberculosis -- South Africa -- Eastern Cape Tuberculosis -- Epidemiology -- Statistics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9648 , vital:34813
- Description: This dissertation is concerned with evolving and extending statistical models in the area of Bayesian spatial modelling, an increasingly important field of spatial epidemiology with particular interest towards application to Tuberculosis data in the Eastern Cape province of South Africa. In spatial epidemiology, the diseases to be examined usually occur within a map that needs spatial statistical methods that are appropriate, to model the observed data in the presence of some covariates and also cater for the variation of the disease. In this thesis, the Bayesian models were developed in such a way that they allowed several factors classified as fixed and random effects, to be included in the models and using the Bayesian approach. The basic model used in disease mapping is the Besag, York and Mollie model, which incorporates two random effects; one which is spatially structured and the other random effect which is spatially unstructured. The effects (fixed and random) were the covariate effects, socio-economic and demographic variability and the spatial variability respectively, which were all investigated in seven different hierarchical/multilevel Bayesian models. These factors showed varying and substantial effects in the posterior relative risk estimation of the disease. We assumed a negative binomial and generalized Poisson distributions to the response variable or relative risk estimate, 𝑦𝑖 ,to capture the over-dispersion phenomenon that is common and inherent with Poisson density for counts data. Spatial and non-spatial models were developed to model over-dispersion with all the distributions; Poisson, negative binomial and generalized Poisson. Negative binomial and generalized Poisson showed varying properties from comparisons with DIC values and parameter estimates to standard errors, which made either of them fit depending on the choice of model selection. It was found that a lower DIC value could be insufficient to determine a best fit model, if other models present estimates with lower variances even at higher DIC values. The generalized Poisson, a two parameter distribution like the negative binomial, which also has the ability to capture both under-dispersion and over-dispersion, was found to perform better in the results than the negative binomial on the basis of a lower variance and with more exact parameter estimates. A new weighted prior distribution, the “Besag2” ICAR model for the structured spatial random effects, which is an extension of the traditional ICAR prior model with two hyperparameters, was also developed and compared with some existing prior models; BYM and ICAR, to measure for spatial dependency in the regions. This new prior distribution was found to show a better fit, when compared to the basic ICAR prior usually assumed for the spatial random effect in the BYM model. This newly parameterized prior distribution in the Besag, York and Mollie model also led to improved parameter control, as the hyperparameters can be seen independently from each other. The result also showed that the new model performed well, both presenting good learning abilities and good shrinkage behaviour. In terms of model choice criteria, the proposed model performed at least equally well and better than the existing models, and the new formulation also gave parameters that are interpretable and have a clearer meaning. To interpolate scattered or regularly distributed data, there are imprecise or exact methods, but there are some of these methods that could be used for interpolating data in a regular grid and others in an irregular grid. Linear and biharmonic spline methods were implemented in MATLAB, to compare for smoothing in the distribution patterns of tuberculosis in the province. This smoothing spline is a method of fitting a smooth curve to a set of noisy observations using a spline function. This new method is rarely used in disease mapping applications, but it has a superior advantage to be assessed at subjective locations rather than only on a rectangular grid as seen in most traditional GIS methods of geospatial analyses. The proposed new models and methods in this thesis were found to be flexible and robust, since they can be reduced or extended according to the nature of the data. Nevertheless, great care must be considered in the choice of prior densities. The approaches developed in this dissertation helped to broaden the scope for spatial analysis and disease mapping applications in epidemiology and public health studies.
- Full Text:
- Date Issued: 2018
Behaviour of quiet time ionospheric disturbances at African equatorial and midlatitude regions
- Authors: Orford, Nicola Diane
- Date: 2018
- Subjects: Ionospheric storms , Ionospheric storms -- Africa , Ionosphere , Plasmasphere , Q-disturbances , Total electron content (TEC)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62672 , vital:28228
- Description: Extreme ionospheric and geomagnetic disturbances affect technology adversely. Prestorm enhancements, considered a potential predictor of geomagnetic storms, occur during quiet conditions prior to geomagnetic disturbances. The ionosphere experiences general disturbances during quiet geomagnetic conditions and these Q- disturbances remain unexplored over Africa. This study used TEC data to characterize the morphology of Q-disturbances over Africa, exploring variations with solar cycle, season, time of occurrence and latitude. Observations from 10 African GPS stations in the equatorial and midlatitude regions show that Q-disturbances in the equatorial region are predominantly driven by E x B variations, while multiple mechanisms affect the midlatitude region. Q- disturbances occur more frequently during nighttime than during daytime and no seasonal trend is observed. Midlatitude Q-disturbance mechanisms are explored in depth, considering substorm activity, the plasmaspheric contribution to GPS TEC and plasma transfer between conjugate points. Substorm activity is not a dominant mechanism, although Q-disturbances occurring under elevated substorm conditions tend to have longer duration and larger amplitude than general Q-disturbances. Many observed Q-disturbances become non-significant once the plasmaspheric contribution to the TEC measurements is removed, indicating that these disturbances occur within the plasmasphere, and not the ionosphere. Transfer of plasma between conjugate points does not seem to be a mechanism driving Q-disturbances, as the corresponding nighttime behaviour expected between depletions in the summer hemisphere and enhancements in the winter hemisphere is not observed. Pre-storm enhancements occur infrequently, rendering them a poor predictor of geomagnetic disturbances. Pre-storm enhancement morphology does not differ significantly from general quiet time enhancement morphology, suggesting pre-storms are not a special case of Q-disturbances.
- Full Text:
- Date Issued: 2018
- Authors: Orford, Nicola Diane
- Date: 2018
- Subjects: Ionospheric storms , Ionospheric storms -- Africa , Ionosphere , Plasmasphere , Q-disturbances , Total electron content (TEC)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62672 , vital:28228
- Description: Extreme ionospheric and geomagnetic disturbances affect technology adversely. Prestorm enhancements, considered a potential predictor of geomagnetic storms, occur during quiet conditions prior to geomagnetic disturbances. The ionosphere experiences general disturbances during quiet geomagnetic conditions and these Q- disturbances remain unexplored over Africa. This study used TEC data to characterize the morphology of Q-disturbances over Africa, exploring variations with solar cycle, season, time of occurrence and latitude. Observations from 10 African GPS stations in the equatorial and midlatitude regions show that Q-disturbances in the equatorial region are predominantly driven by E x B variations, while multiple mechanisms affect the midlatitude region. Q- disturbances occur more frequently during nighttime than during daytime and no seasonal trend is observed. Midlatitude Q-disturbance mechanisms are explored in depth, considering substorm activity, the plasmaspheric contribution to GPS TEC and plasma transfer between conjugate points. Substorm activity is not a dominant mechanism, although Q-disturbances occurring under elevated substorm conditions tend to have longer duration and larger amplitude than general Q-disturbances. Many observed Q-disturbances become non-significant once the plasmaspheric contribution to the TEC measurements is removed, indicating that these disturbances occur within the plasmasphere, and not the ionosphere. Transfer of plasma between conjugate points does not seem to be a mechanism driving Q-disturbances, as the corresponding nighttime behaviour expected between depletions in the summer hemisphere and enhancements in the winter hemisphere is not observed. Pre-storm enhancements occur infrequently, rendering them a poor predictor of geomagnetic disturbances. Pre-storm enhancement morphology does not differ significantly from general quiet time enhancement morphology, suggesting pre-storms are not a special case of Q-disturbances.
- Full Text:
- Date Issued: 2018
Beneficiary perceptions regarding farm worker equity share schemes in South Africa
- Authors: Xolo, Siyavuya Nicholas
- Date: 2018
- Subjects: Investments -- South Africa , Investment analysis Land reform -- South Africa Agricultural laborers -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/36323 , vital:33920
- Description: The study aimed to assess beneficiary perceptions regarding farm worker equity share schemes in South Africa. Farm worker equity share schemes started in the early 1990s with the aim of having a special arrangement pertaining to the ownership and operation of a farm between the farmer and the farm workers. This was intended to assist farm workers in not only remaining as farm workers but also to gain ownership of the farm. This could lead to the empowerment of farm workers, better working conditions, improved living standards and their rights to land ownership. Farm worker equity share schemes have been widely reported as having failed to meet their intended objectives, thus, in 2009, the Department of Rural Development and Land Reform initiated a study to assess the implementation of equity share schemes. Although the report was not made public, it reports that out of the 88 equity share schemes at the time, only nine managed to pay dividends ranging from R200 to R2000 per year. As a result, the Department of Rural Development and Land Reform put a moratorium on equity share schemes; this was however removed in 2011. It is against this background that this study was undertaken to assess beneficiaries’ perceptions regarding farm worker equity share schemes in South Africa. A positivistic research paradigm was employed in this study, by means of quantitative research. Secondary data in the form of textbooks, journal articles and Internet sources provided the theoretical framework for this study. Primary data was obtained using the survey method, by means of self-administered structured questionnaires. Convenience and purposive sampling were applied in order to select 20 farms that use equity share schemes. The farms selected for this study were located in the Eastern Cape, Gauteng, Mpumalanga and the Western Cape provinces, covering a variety of farming activities such as citrus fruits, crops, vegetables and wineries. Ideally, a total of 15 farm workers per farm were targeted. However, due to a variation in these farm worker equity share schemes, on some farms less than 15 workers and in others more than 15 workers were selected for the study; this resulted in a total sample size of 341 farm workers. Ten null-hypotheses and a hypothetical model of beneficiary perceptions regarding farm worker equity share schemes were tested. The influence of seven independent variables were tested, these are: stakeholder trust, operational risks, government interventions, two-way communication, farm worker empowerment, training and skills development, and access to resources on farm worker equity share schemes. In addition, the influence of farm worker equity share schemes on three dependent variables, namely, farming performance, sustainability and employee expectations were tested. The Statistica (version 13.2) computer programme was used to analyse the results by means of advanced statistical techniques (such as exploratory factor analysis, regression and correlation analyses) as well as descriptive analysis and frequency distributions. After various statistical procedures, the model was re-specified; some of the variables were then renamed and the hypotheses were adjusted accordingly. The empirical results showed that three key variables to the success of farm worker equity share schemes are stakeholder trust, government interventions and farm owner support. It was determined that these key variables could lead to increased farming performance, farming sustainability and meeting employee expectations. This study provided useful and practical guidelines to farm owners and administrators of equity share schemes, so as to ensure effective strategising that could enhance their competitiveness and long term survival. The findings of this study could inform strategy policy formulation and implementation in the agricultural sector, as pertaining to farm worker equity share schemes. The study used a sound and well-developed research design and methodology, which has been justified and successfully applied to this research; this method can be utilised by other similar studies to conduct empirical research in the field of farm worker equity share schemes. It is envisaged that the results and recommendations of this study could be used to implement effective strategies that could ensure the effective functioning of farm worker equity share schemes in South Africa.
- Full Text:
- Date Issued: 2018
- Authors: Xolo, Siyavuya Nicholas
- Date: 2018
- Subjects: Investments -- South Africa , Investment analysis Land reform -- South Africa Agricultural laborers -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/36323 , vital:33920
- Description: The study aimed to assess beneficiary perceptions regarding farm worker equity share schemes in South Africa. Farm worker equity share schemes started in the early 1990s with the aim of having a special arrangement pertaining to the ownership and operation of a farm between the farmer and the farm workers. This was intended to assist farm workers in not only remaining as farm workers but also to gain ownership of the farm. This could lead to the empowerment of farm workers, better working conditions, improved living standards and their rights to land ownership. Farm worker equity share schemes have been widely reported as having failed to meet their intended objectives, thus, in 2009, the Department of Rural Development and Land Reform initiated a study to assess the implementation of equity share schemes. Although the report was not made public, it reports that out of the 88 equity share schemes at the time, only nine managed to pay dividends ranging from R200 to R2000 per year. As a result, the Department of Rural Development and Land Reform put a moratorium on equity share schemes; this was however removed in 2011. It is against this background that this study was undertaken to assess beneficiaries’ perceptions regarding farm worker equity share schemes in South Africa. A positivistic research paradigm was employed in this study, by means of quantitative research. Secondary data in the form of textbooks, journal articles and Internet sources provided the theoretical framework for this study. Primary data was obtained using the survey method, by means of self-administered structured questionnaires. Convenience and purposive sampling were applied in order to select 20 farms that use equity share schemes. The farms selected for this study were located in the Eastern Cape, Gauteng, Mpumalanga and the Western Cape provinces, covering a variety of farming activities such as citrus fruits, crops, vegetables and wineries. Ideally, a total of 15 farm workers per farm were targeted. However, due to a variation in these farm worker equity share schemes, on some farms less than 15 workers and in others more than 15 workers were selected for the study; this resulted in a total sample size of 341 farm workers. Ten null-hypotheses and a hypothetical model of beneficiary perceptions regarding farm worker equity share schemes were tested. The influence of seven independent variables were tested, these are: stakeholder trust, operational risks, government interventions, two-way communication, farm worker empowerment, training and skills development, and access to resources on farm worker equity share schemes. In addition, the influence of farm worker equity share schemes on three dependent variables, namely, farming performance, sustainability and employee expectations were tested. The Statistica (version 13.2) computer programme was used to analyse the results by means of advanced statistical techniques (such as exploratory factor analysis, regression and correlation analyses) as well as descriptive analysis and frequency distributions. After various statistical procedures, the model was re-specified; some of the variables were then renamed and the hypotheses were adjusted accordingly. The empirical results showed that three key variables to the success of farm worker equity share schemes are stakeholder trust, government interventions and farm owner support. It was determined that these key variables could lead to increased farming performance, farming sustainability and meeting employee expectations. This study provided useful and practical guidelines to farm owners and administrators of equity share schemes, so as to ensure effective strategising that could enhance their competitiveness and long term survival. The findings of this study could inform strategy policy formulation and implementation in the agricultural sector, as pertaining to farm worker equity share schemes. The study used a sound and well-developed research design and methodology, which has been justified and successfully applied to this research; this method can be utilised by other similar studies to conduct empirical research in the field of farm worker equity share schemes. It is envisaged that the results and recommendations of this study could be used to implement effective strategies that could ensure the effective functioning of farm worker equity share schemes in South Africa.
- Full Text:
- Date Issued: 2018
Biochemical characterisation and small molecule modulation of the interaction between two cytosolic Hsp70s from Trypanosoma brucei and potential co-chaperones
- Authors: Bentley, Stephen John
- Date: 2018
- Subjects: Uncatalogued
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63402 , vital:28407
- Description: Expected release date-April 2019
- Full Text:
- Date Issued: 2018
- Authors: Bentley, Stephen John
- Date: 2018
- Subjects: Uncatalogued
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63402 , vital:28407
- Description: Expected release date-April 2019
- Full Text:
- Date Issued: 2018