Molecular studies on cholera and non-cholera causing vibrio pathogens isolated from some water resources and aquatic animals in Eastern Cape Province, South Africa
- Authors: Abioye, Oluwatayo Emmanuel
- Date: 2018
- Subjects: Vibrio cholerae -- Pathogens Vibrio cholerae
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9154 , vital:34283
- Description: The global socioeconomic and disease burdens of cholera; a disease caused by Vibrio cholerae have been ongoing since it was first reported in India in 1563. Unfortunately, emerging non-cholera causing Vibrio pathogens have been contributing to the malady of Vibrio genus globally in the recent years. The history of cholera outbreaks in South Africa have been well documented but information regarding other non-cholera Vibrio pathogens, is deficient in the literature. This study evaluated the statuses of aquatic animals including crustaceans, molluscs, and fish as important reservoirs of six key pathogenic Vibrio spp. in some important water resources and fish markets in the Eastern Cape, South Africa, between December 2016 and November 2017. Physiochemical parameters that are vital for water quality assessment were determined for all the water sampling points using standard methods. The parameters include pH, electrical conductivity (EC), total dissolved solids (TDS), salinity, temperature, total dissolved solids, turbidity and biological oxygen demand (BOD5). Vibrio spp. densities in water and aquatic animal samples were determined using membrane filtration and Most Probable NumberPolymerase chain reaction (MPN-PCR) methods respectively. Presumptive Vibrio spp. were recovered from the samples by direct plating of water samples and aliquots of aquatic animal homogenates on thiosulfate-citrate-bile salts-sucrose (TCBS) agar. Where necessary, samples were enriched using alkaline peptone water (APW) for optimum isolation of the targeted Vibrio spp. Presumptive isolates were identified and delineated into the six targeted pathogenic Vibrio spp. using PCR method. The antibiogram fingerprint of selected Vibrio spp. against a panel 18 panels of antibacterial agents were also carried out. Multiple antibiotic resistance phenotypes (MARPs) per set of isolates from water sampling sites and aquatic animal types were determined. Finally, multiple antibiotic resistance indices (MARI) for isolates recovered from the samples were determined. All experiments were done in replicate and data compared using one-way analysis of variance and one-tail t-test as appropriate. Correlation and regression analysis were also carried out as appropriate. Uncontrolled agricultural, industrial and domestic wastewater influxes, as well as run-off intrusions into the water resources investigated, were observed throughout the sampling season. The physicochemical qualities of the water resources varied with seasons. While pH observed for all the water sampling sites complied with South Africa and international standards, most sampling sites did not comply with set standards for one or more of the remaining physicochemical parameters. The parameters that frequently exhibited significant correlation and regression with Vibrio spp. densities were TDS, TSS, turbidity and temperature. The densities of Vibrio spp. in the samples studied varied with season and the highest density per sample types were observed in the summer. The prevalence of Vibrio spp. in freshwater, brackish water and aquatic animal samples were 79 percent, 78 percent and 86 percent respectively. The respective prevalence of V. cholerae, V. mimicus, V. fluvialis, V.alginolyticus, V. vulnificus and V. parahaemolyticus in freshwater samples (18 percent, 3 percent, ~1 percent, ~1 percent, <1 percent, < percent), brackish water(12 percent, 3 percent, 28 percent,~1 percent, ~1 percent, 9 percent) and aquatic animals (9 percent, 1 percent, 17 percent, 1 percent, 1 percent, 17 percent) were observed. All isolates from all the sample types showed very high antibiotic resistance against ampicillin and polymyxin B. Furthermore, MARPs were observed among isolates from the three sample types. Multiple antibiotic resistance indices (MARI) above 0.2 was observed at one site on Kowie River, three on Kubusi River and one on Buffalo River. The findings of this study suggest that most South Africa water-bodies especially freshwater resources are not well protected. This need to be addressed in a country like South Africa where availability of the freshwater resource for human activities is a critical issue. Base on the prevalence of pathogenic Vibrio spp. especially MARPs and sites that exhibited above 0.2 threshold for MARI, environmental base Vibrio species monitoring programs are recommended.
- Full Text:
- Date Issued: 2018
- Authors: Abioye, Oluwatayo Emmanuel
- Date: 2018
- Subjects: Vibrio cholerae -- Pathogens Vibrio cholerae
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9154 , vital:34283
- Description: The global socioeconomic and disease burdens of cholera; a disease caused by Vibrio cholerae have been ongoing since it was first reported in India in 1563. Unfortunately, emerging non-cholera causing Vibrio pathogens have been contributing to the malady of Vibrio genus globally in the recent years. The history of cholera outbreaks in South Africa have been well documented but information regarding other non-cholera Vibrio pathogens, is deficient in the literature. This study evaluated the statuses of aquatic animals including crustaceans, molluscs, and fish as important reservoirs of six key pathogenic Vibrio spp. in some important water resources and fish markets in the Eastern Cape, South Africa, between December 2016 and November 2017. Physiochemical parameters that are vital for water quality assessment were determined for all the water sampling points using standard methods. The parameters include pH, electrical conductivity (EC), total dissolved solids (TDS), salinity, temperature, total dissolved solids, turbidity and biological oxygen demand (BOD5). Vibrio spp. densities in water and aquatic animal samples were determined using membrane filtration and Most Probable NumberPolymerase chain reaction (MPN-PCR) methods respectively. Presumptive Vibrio spp. were recovered from the samples by direct plating of water samples and aliquots of aquatic animal homogenates on thiosulfate-citrate-bile salts-sucrose (TCBS) agar. Where necessary, samples were enriched using alkaline peptone water (APW) for optimum isolation of the targeted Vibrio spp. Presumptive isolates were identified and delineated into the six targeted pathogenic Vibrio spp. using PCR method. The antibiogram fingerprint of selected Vibrio spp. against a panel 18 panels of antibacterial agents were also carried out. Multiple antibiotic resistance phenotypes (MARPs) per set of isolates from water sampling sites and aquatic animal types were determined. Finally, multiple antibiotic resistance indices (MARI) for isolates recovered from the samples were determined. All experiments were done in replicate and data compared using one-way analysis of variance and one-tail t-test as appropriate. Correlation and regression analysis were also carried out as appropriate. Uncontrolled agricultural, industrial and domestic wastewater influxes, as well as run-off intrusions into the water resources investigated, were observed throughout the sampling season. The physicochemical qualities of the water resources varied with seasons. While pH observed for all the water sampling sites complied with South Africa and international standards, most sampling sites did not comply with set standards for one or more of the remaining physicochemical parameters. The parameters that frequently exhibited significant correlation and regression with Vibrio spp. densities were TDS, TSS, turbidity and temperature. The densities of Vibrio spp. in the samples studied varied with season and the highest density per sample types were observed in the summer. The prevalence of Vibrio spp. in freshwater, brackish water and aquatic animal samples were 79 percent, 78 percent and 86 percent respectively. The respective prevalence of V. cholerae, V. mimicus, V. fluvialis, V.alginolyticus, V. vulnificus and V. parahaemolyticus in freshwater samples (18 percent, 3 percent, ~1 percent, ~1 percent, <1 percent, < percent), brackish water(12 percent, 3 percent, 28 percent,~1 percent, ~1 percent, 9 percent) and aquatic animals (9 percent, 1 percent, 17 percent, 1 percent, 1 percent, 17 percent) were observed. All isolates from all the sample types showed very high antibiotic resistance against ampicillin and polymyxin B. Furthermore, MARPs were observed among isolates from the three sample types. Multiple antibiotic resistance indices (MARI) above 0.2 was observed at one site on Kowie River, three on Kubusi River and one on Buffalo River. The findings of this study suggest that most South Africa water-bodies especially freshwater resources are not well protected. This need to be addressed in a country like South Africa where availability of the freshwater resource for human activities is a critical issue. Base on the prevalence of pathogenic Vibrio spp. especially MARPs and sites that exhibited above 0.2 threshold for MARI, environmental base Vibrio species monitoring programs are recommended.
- Full Text:
- Date Issued: 2018
Decentralization and quality assurance in the Ugandan primary education sector
- Authors: Abu-Baker, Mutaaya Sirajee
- Date: 2018
- Subjects: Schools -- Decentralization -- Uganda , Education, Primary -- Uganda , Educational change -- Uganda
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/57390 , vital:26897
- Description: The study presented in this thesis is a case study analysis of decentralization and quality assurance in a decentralized set up of the Ugandan Primary Schooling. The research looked at how the monitoring and evaluation informed the policy formulation process to regulate quality assurance in a decentralized governance of primary education. The Study was positioned in the critical realist paradigm, interpretive in orientation and used both coding and thematic techniques to understand the teachers’, SMC members’, and officers’ (at district and ministry levels) experiences and perceptions of quality assurance in a decentralized set up. Data was gathered using interviews, document analysis and observation methods. The findings indicated that the study was affected by eleven themes: Management System and Leadership, Human Resource Management, Finance Administration and Management, Parenting and Nutrition, Politics, Motivation, Social Structures and Patterns, Legislative Process and Policies, Infrastructure Development and Management, Community Involvement in Education and Curriculum and Professionalism. The monitoring and evaluation system had a framework in which it operates, though there was no quality assurance policy to guide the provision of quality education. The study finally indicated that there are more threats in a decentralized set up that put Quality in danger. Secondly, there was absence of supervision/inspection in schools as there was no evidence to prove this due to absence of reports. However, document analysis indicated visits of officers to schools. Records management was a problem to schools. Decentralization was adopted at different levels by different countries to address specific problems identified in view of service delivery. Finally, though monitoring and evaluation results informed the policy and decision makers, there was no quality assurance policy to guide the provision of quality education in institutions.
- Full Text:
- Date Issued: 2018
- Authors: Abu-Baker, Mutaaya Sirajee
- Date: 2018
- Subjects: Schools -- Decentralization -- Uganda , Education, Primary -- Uganda , Educational change -- Uganda
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/57390 , vital:26897
- Description: The study presented in this thesis is a case study analysis of decentralization and quality assurance in a decentralized set up of the Ugandan Primary Schooling. The research looked at how the monitoring and evaluation informed the policy formulation process to regulate quality assurance in a decentralized governance of primary education. The Study was positioned in the critical realist paradigm, interpretive in orientation and used both coding and thematic techniques to understand the teachers’, SMC members’, and officers’ (at district and ministry levels) experiences and perceptions of quality assurance in a decentralized set up. Data was gathered using interviews, document analysis and observation methods. The findings indicated that the study was affected by eleven themes: Management System and Leadership, Human Resource Management, Finance Administration and Management, Parenting and Nutrition, Politics, Motivation, Social Structures and Patterns, Legislative Process and Policies, Infrastructure Development and Management, Community Involvement in Education and Curriculum and Professionalism. The monitoring and evaluation system had a framework in which it operates, though there was no quality assurance policy to guide the provision of quality education. The study finally indicated that there are more threats in a decentralized set up that put Quality in danger. Secondly, there was absence of supervision/inspection in schools as there was no evidence to prove this due to absence of reports. However, document analysis indicated visits of officers to schools. Records management was a problem to schools. Decentralization was adopted at different levels by different countries to address specific problems identified in view of service delivery. Finally, though monitoring and evaluation results informed the policy and decision makers, there was no quality assurance policy to guide the provision of quality education in institutions.
- Full Text:
- Date Issued: 2018
Graphene quantum dots and their metallophthalocyanines nanoconjugates as novel photoluminescent nanosensors
- Authors: Achadu, Ojodomo John
- Date: 2018
- Subjects: Quantum dots , Graphene , Phthalocyanines , Nanoconjugates , Novel photoluminescent nanosensors , Metallophthalocyanines
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/60719 , vital:27821
- Description: The fabrication and application of graphene quantum dots (GQDs)-based photoluminescent probes for the detection of analytes is presented. GQDs were functionalized with complexes such as metallophthalocyanines (MPcs), metal nanoparticles (Au@Ag NPs), 2,2,6,6-tetramethyl(piperidin-1-yl)oxyl (TEMPO), maleimide and thymine for the sensing of target analytes such as ascorbic acid (AA), biothiols (cysteine, homocysteine and glutathione) and mercury ion (Hg²+). The design strategy and approach was based on the quenching of the fluorescence of the GQDs upon functionalization with the above-mentioned complexes, which could be restored in the presence of the target analytes (due to their specific interaction affinity with the complexes). For the detection of AA, GQDs were covalently and/or non-covalently conjugated to TEMPO-bearing complexes to form GQDs-4A-TEMPO and GQDs-TEMPO-MPc systems with nanomolar limits of detection. For the detection of biothiols, Au@Ag NPs and maleimide-bearing complexes (MPc), which have specific affinity to interact with biothiols, were deployed. Hg²+ detection involved the use of GQDs and/or MPcs with thiol and thymine groups, respectively. In addition, a smart sensing platform was designed for the dual detection of biothiols and Hg²+ using supramolecular hybrid of polyethyleneimine functionalized-GQDs and MPc-Au@Ag conjugate. The probe could detect, in a sequential manner, Hg²+ and biothiols with high sensitivity. Results obtained from the LODs of the probes showed that GQDs sensing performances could be enhanced in the presence of MPcs. The probes designed in this work were successfully deployed in the assays of the target analytes in real samples and the recoveries obtained confirmed the analytical applicability of the probes.
- Full Text:
- Date Issued: 2018
- Authors: Achadu, Ojodomo John
- Date: 2018
- Subjects: Quantum dots , Graphene , Phthalocyanines , Nanoconjugates , Novel photoluminescent nanosensors , Metallophthalocyanines
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/60719 , vital:27821
- Description: The fabrication and application of graphene quantum dots (GQDs)-based photoluminescent probes for the detection of analytes is presented. GQDs were functionalized with complexes such as metallophthalocyanines (MPcs), metal nanoparticles (Au@Ag NPs), 2,2,6,6-tetramethyl(piperidin-1-yl)oxyl (TEMPO), maleimide and thymine for the sensing of target analytes such as ascorbic acid (AA), biothiols (cysteine, homocysteine and glutathione) and mercury ion (Hg²+). The design strategy and approach was based on the quenching of the fluorescence of the GQDs upon functionalization with the above-mentioned complexes, which could be restored in the presence of the target analytes (due to their specific interaction affinity with the complexes). For the detection of AA, GQDs were covalently and/or non-covalently conjugated to TEMPO-bearing complexes to form GQDs-4A-TEMPO and GQDs-TEMPO-MPc systems with nanomolar limits of detection. For the detection of biothiols, Au@Ag NPs and maleimide-bearing complexes (MPc), which have specific affinity to interact with biothiols, were deployed. Hg²+ detection involved the use of GQDs and/or MPcs with thiol and thymine groups, respectively. In addition, a smart sensing platform was designed for the dual detection of biothiols and Hg²+ using supramolecular hybrid of polyethyleneimine functionalized-GQDs and MPc-Au@Ag conjugate. The probe could detect, in a sequential manner, Hg²+ and biothiols with high sensitivity. Results obtained from the LODs of the probes showed that GQDs sensing performances could be enhanced in the presence of MPcs. The probes designed in this work were successfully deployed in the assays of the target analytes in real samples and the recoveries obtained confirmed the analytical applicability of the probes.
- Full Text:
- Date Issued: 2018
Active learning strategies used by teachers during English reading comprehension lessons in six selected primary schools in Nigeria
- Adepoju, Adetokunboh Abayomi
- Authors: Adepoju, Adetokunboh Abayomi
- Date: 2018
- Subjects: Education, Primary -- Nigeria Learning strategies -- Nigeria
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/8485 , vital:32838
- Description: Reading is an integral part of education from which one gets access to information. Unfortunately, reading ability among primary school pupils, the foundation level of education, is at its lowest ebb. These pupils when they get to secondary schools eventually fail external examinations-a situation which according to National Examination Council, is caused by inappropriate teaching methods. Consequent upon this, this study assessed active learning strategies used by teacher during English reading comprehension lessons in six selected primary schools in Nigeria. The main research question was: How do teachers employ active learning strategies in teaching English reading comprehension? The study adopted qualitative approach and case study design. With the use of purposive sampling technique to select six head teachers, six primary VI teachers, two members of the School Basic Committee, the Director Local Government Education Authority and six focus groups. The study used interviews, documents and focus group interview as research instruments to collect data. Data were analysed based on interpretivism paradigm which interpreted participants’ views according to the word view of the participants. The study found out that teachers have good knowledge of active learning strategies and use them in teaching English reading comprehension lessons but are faced with some daunting challenges such as non-availability of instructional materials, lack of library, lack of qualified teachers and lack of time. The study concluded that students fail woefully in English reading comprehension because teachers do not use appropriate teaching techniques. The study recommend that teachers must use active learning strategies effectively and more often during English reading comprehension, more time should be allocated for English reading comprehension, effective use of instructional materials should be considered and the government should employ specialist teachers to teach English reading comprehension.
- Full Text:
- Date Issued: 2018
- Authors: Adepoju, Adetokunboh Abayomi
- Date: 2018
- Subjects: Education, Primary -- Nigeria Learning strategies -- Nigeria
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/8485 , vital:32838
- Description: Reading is an integral part of education from which one gets access to information. Unfortunately, reading ability among primary school pupils, the foundation level of education, is at its lowest ebb. These pupils when they get to secondary schools eventually fail external examinations-a situation which according to National Examination Council, is caused by inappropriate teaching methods. Consequent upon this, this study assessed active learning strategies used by teacher during English reading comprehension lessons in six selected primary schools in Nigeria. The main research question was: How do teachers employ active learning strategies in teaching English reading comprehension? The study adopted qualitative approach and case study design. With the use of purposive sampling technique to select six head teachers, six primary VI teachers, two members of the School Basic Committee, the Director Local Government Education Authority and six focus groups. The study used interviews, documents and focus group interview as research instruments to collect data. Data were analysed based on interpretivism paradigm which interpreted participants’ views according to the word view of the participants. The study found out that teachers have good knowledge of active learning strategies and use them in teaching English reading comprehension lessons but are faced with some daunting challenges such as non-availability of instructional materials, lack of library, lack of qualified teachers and lack of time. The study concluded that students fail woefully in English reading comprehension because teachers do not use appropriate teaching techniques. The study recommend that teachers must use active learning strategies effectively and more often during English reading comprehension, more time should be allocated for English reading comprehension, effective use of instructional materials should be considered and the government should employ specialist teachers to teach English reading comprehension.
- Full Text:
- Date Issued: 2018
Incidence of porcine circovirus type 2 and porcine parvoviruses in swine herds of some communities in Eastern Cape, South Africa
- Authors: Afolabi, Kayode Olayinka
- Date: 2018
- Subjects: Swine -- Diseases Swine -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9691 , vital:34820
- Description: Porcine circovirus type 2 (PCV2) is one of the swine pathogens of global economic importance. Since its first detection in early 1990s as the main etiologic agent of porcine multisystemic wasting syndrome (PMWS) and many other porcine circovirus-associated diseases (PCVAD), the virus has been extensively studied and has been found to be present in virtually all the pig producing countries of the world. As a viral pathogen that brings about clinical diseases aided by co-infecting pathogens, the involvement of many other viral agents including porcine parvoviruses (PPVs) have caught the attention of stakeholders worldwide. However, no surveillance study of the viral pathogens has been carried out in South Africa as there are little or no information on their prevalence in the swine herds of the country. This present study therefore aimed at detection and molecular characterization of PCV2 and PPVs in swine herds of some selected communities in Eastern Cape Province, South Africa. A total of 375 field samples were collected from seven commercial and communal farms from three District Municipalities of Eastern Cape, South Africa between 2015 and 2016. Structured questionnaires were also administered to each farm at the time of sample collection to obtain some important information relating to health status and farm management practices in the sampled farms. With the aid of conventional PCR method, 339 samples were initially screened for the presence of PCV2; positive amplicons were sequenced and obtained partial genomes of the virus were preliminarily analyzed. In order to obtain the complete genomes of the virus, four overlapping primer pairs were used to amplify the full-genome of PCV2 from the initial positive samples; amplified genomes were sequenced using the Sanger methods, sequenced PCV2 genomes were assembled and characterized. Furthermore, the prevalences of some designated PPVs in the sampled farms were obtained using 110 samples randomly selected from the previously archived samples and screened with 6 different primer pairs specific for the detection of 7 PPVs. All the amplified parvoviruses’ genomes were sequenced; their sequenced partial genomes were subsequently base-culled and analysed. The data obtained revealed that 54/339 (15.93 percent) samples from the swine herds were positive for PCV2; whereas the degree of occurrence of the viral pathogen as observed at farm level ranges from approximately 5.6 to 60 percent. The majority 15/17 (88 percent) of the analyzed partial sequences were found clustering with other PCV2b strains in the phylogenetic analysis. More interestingly, two other sequences obtained were also found clustering within PCV2d genotype in the initial screening and analysis. Furthermore, 15 complete PCV2 genomes were successfully amplified, sequenced and assembled. NJ and ML phylogenetic analysis of the complete ORF2 gene and full genomes unanimously showed 11 of the assembled genomes belonging to genotype PCV2b. Another 3 of the characterized sequences formed clade with other reference mutant PCV2b and PCV2b subtype 1C (PCV2d) strains from different parts of the world. The last sequence however, clustered with other reference strains belonging to PCV2 intermediate clade 2 (PCV2-IM2) recently identified in a global PCV2 strains phylogenetic analysis. Other genetic analyses including multiple sequence alignment and p-distance analysis also confirmed the outcomes of the phylogenetic analyses of the complete capsid gene and fullgenomes of the virus. On the other hand, the findings of the molecular profiling for PPVs showed that all the screened parvoviruses were present in the study area, having prevalence of 29.1 percent (PPV1), 21.8 percent (PPV2), 5.5 percent (PPV3), 43.6 percent (PPV4), 21.8 percent (PBo-likeV) and 44.6 percent for PBoV1 and PBoV2. Double infection of the screened PPVs was observed to be very rampant among the pigs as high as 20/110 (18.2 percent) for PPV2/PPV4 and PPV4/PBoV; followed by 19/110 (17.3 percent) of the samples for PPV1/PPV4 and PPV1/PBoV. Three of the viruses were found simultaneously in 19 of the screened samples representing 17.3 percent, whereas 8 (7.3 percent) samples were positive for four of the viruses. Phylogenetic analyses of PPV1, PPV2 and PBoVs 1 and 2 were conducted with two major clades homologous for each of them. This is the first report of PCV2 in swine herds of the Province and the first detection of PCV2b, PCV2d and PCV2-IM2 strains in South African swine herds. It follows the first reported case of PCV2a in an outbreak of porcine multisystemic wasting syndrome (PMWS) in Gauteng Province, South Africa over two decades ago. Also, this is the first major epidemiologic study on PPVs in the country following the initial case study of 1975. These findings confirmed the presence of the allimportant viral pathogens among pigs and also give preliminary insights into the possibility of co-infections of the pathogens in the studied area. This could however result in a serious large scale outbreak of devastating disease(s) associated with the viral pathogens, thereby ultimately resulting in huge economic losses if no appropriate measures are taken to effectively curb their spread across the country.
- Full Text:
- Date Issued: 2018
- Authors: Afolabi, Kayode Olayinka
- Date: 2018
- Subjects: Swine -- Diseases Swine -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9691 , vital:34820
- Description: Porcine circovirus type 2 (PCV2) is one of the swine pathogens of global economic importance. Since its first detection in early 1990s as the main etiologic agent of porcine multisystemic wasting syndrome (PMWS) and many other porcine circovirus-associated diseases (PCVAD), the virus has been extensively studied and has been found to be present in virtually all the pig producing countries of the world. As a viral pathogen that brings about clinical diseases aided by co-infecting pathogens, the involvement of many other viral agents including porcine parvoviruses (PPVs) have caught the attention of stakeholders worldwide. However, no surveillance study of the viral pathogens has been carried out in South Africa as there are little or no information on their prevalence in the swine herds of the country. This present study therefore aimed at detection and molecular characterization of PCV2 and PPVs in swine herds of some selected communities in Eastern Cape Province, South Africa. A total of 375 field samples were collected from seven commercial and communal farms from three District Municipalities of Eastern Cape, South Africa between 2015 and 2016. Structured questionnaires were also administered to each farm at the time of sample collection to obtain some important information relating to health status and farm management practices in the sampled farms. With the aid of conventional PCR method, 339 samples were initially screened for the presence of PCV2; positive amplicons were sequenced and obtained partial genomes of the virus were preliminarily analyzed. In order to obtain the complete genomes of the virus, four overlapping primer pairs were used to amplify the full-genome of PCV2 from the initial positive samples; amplified genomes were sequenced using the Sanger methods, sequenced PCV2 genomes were assembled and characterized. Furthermore, the prevalences of some designated PPVs in the sampled farms were obtained using 110 samples randomly selected from the previously archived samples and screened with 6 different primer pairs specific for the detection of 7 PPVs. All the amplified parvoviruses’ genomes were sequenced; their sequenced partial genomes were subsequently base-culled and analysed. The data obtained revealed that 54/339 (15.93 percent) samples from the swine herds were positive for PCV2; whereas the degree of occurrence of the viral pathogen as observed at farm level ranges from approximately 5.6 to 60 percent. The majority 15/17 (88 percent) of the analyzed partial sequences were found clustering with other PCV2b strains in the phylogenetic analysis. More interestingly, two other sequences obtained were also found clustering within PCV2d genotype in the initial screening and analysis. Furthermore, 15 complete PCV2 genomes were successfully amplified, sequenced and assembled. NJ and ML phylogenetic analysis of the complete ORF2 gene and full genomes unanimously showed 11 of the assembled genomes belonging to genotype PCV2b. Another 3 of the characterized sequences formed clade with other reference mutant PCV2b and PCV2b subtype 1C (PCV2d) strains from different parts of the world. The last sequence however, clustered with other reference strains belonging to PCV2 intermediate clade 2 (PCV2-IM2) recently identified in a global PCV2 strains phylogenetic analysis. Other genetic analyses including multiple sequence alignment and p-distance analysis also confirmed the outcomes of the phylogenetic analyses of the complete capsid gene and fullgenomes of the virus. On the other hand, the findings of the molecular profiling for PPVs showed that all the screened parvoviruses were present in the study area, having prevalence of 29.1 percent (PPV1), 21.8 percent (PPV2), 5.5 percent (PPV3), 43.6 percent (PPV4), 21.8 percent (PBo-likeV) and 44.6 percent for PBoV1 and PBoV2. Double infection of the screened PPVs was observed to be very rampant among the pigs as high as 20/110 (18.2 percent) for PPV2/PPV4 and PPV4/PBoV; followed by 19/110 (17.3 percent) of the samples for PPV1/PPV4 and PPV1/PBoV. Three of the viruses were found simultaneously in 19 of the screened samples representing 17.3 percent, whereas 8 (7.3 percent) samples were positive for four of the viruses. Phylogenetic analyses of PPV1, PPV2 and PBoVs 1 and 2 were conducted with two major clades homologous for each of them. This is the first report of PCV2 in swine herds of the Province and the first detection of PCV2b, PCV2d and PCV2-IM2 strains in South African swine herds. It follows the first reported case of PCV2a in an outbreak of porcine multisystemic wasting syndrome (PMWS) in Gauteng Province, South Africa over two decades ago. Also, this is the first major epidemiologic study on PPVs in the country following the initial case study of 1975. These findings confirmed the presence of the allimportant viral pathogens among pigs and also give preliminary insights into the possibility of co-infections of the pathogens in the studied area. This could however result in a serious large scale outbreak of devastating disease(s) associated with the viral pathogens, thereby ultimately resulting in huge economic losses if no appropriate measures are taken to effectively curb their spread across the country.
- Full Text:
- Date Issued: 2018
Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae) population ecology in citrus orchards: the influence of orchard age
- Authors: Albertyn, Sonnica
- Date: 2018
- Subjects: Cryptophlebia leucotreta , Population biology , Insect populations , Orchards , Insect nematodes , Entomopathogenic fungi
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62615 , vital:28213
- Description: Anecdotal reports in the South African citrus industry claim higher populations of false codling moth (FCM), Thaumatotibia (Cryptophlebia) leucotreta (Meyr) (Lepidoptera: Tortricidae), in orchards during the first three to five harvesting years of citrus planted in virgin soil, after which, FCM numbers seem to decrease and remain consistent. Various laboratory studies and field surveys were conducted to determine if, and why juvenile orchards (four to eight years old) experience higher FCM infestation than mature orchards (nine years and older). In laboratory trials, Washington Navel oranges and Nova Mandarins from juvenile trees were shown to be significantly more susceptible to FCM damage and significantly more attractive for oviposition in both choice and no-choice trials, than fruit from mature trees. Although fruit from juvenile Cambria Navel trees were significantly more attractive than mature orchards for oviposition, they were not more susceptible to FCM damage. In contrast, fruit from juvenile and mature Midnight Valencia orchards were equally attractive for oviposition, but fruit from juvenile trees were significantly more susceptible to FCM damage than fruit from mature trees. Artificial diets were augmented with powder from fruit from juvenile or mature Washington Navel orchards at 5%, 10%, 15% or 30%. Higher larval survival of 76%, 63%, 50% and 34%, respectively, was recorded on diets containing fruit powder from the juvenile trees than on diets containing fruit powder from the mature trees, at 69%, 57%, 44% and 27% larval survival, respectively. Bioassays were conducted to determine if differences in plant chemistry between fruit from juvenile and mature trees will have an impact on the susceptibility FCM to entomopathogenic nematodes (EPN), entomopathogenic fungi (EPF) and Cryptophlebia leucotreta granulovirus (CrleGV). No significant differences in the susceptibility of larvae reared on diets containing 15% fruit powder from juvenile and mature trees to EPN and EPF were recorded. Mortality of neonate larvae was significantly lower when placed on diets containing 15% fruit powder from mature trees (45% mortality) than diets containing 15% fruit powder from juvenile trees (61% mortality), after larvae ingested the lowest virus concentration tested, being 2 x104 OBs/ml. Data collected from field surveys showed significantly lower egg parasitism, virus infection of larvae and EPF occurrence in juvenile orchards than mature orchards. Egg parasitism was between 11% and 54% higher in mature orchards than juvenile orchards, with the exception of Mandarins during 2015, where egg parasitism was slightly higher in juvenile orchards, but not significantly so. A significantly higher proportion of larvae retrieved from mature orchards (7% of larvae) were infected with CrleGV than larvae retrieved from juvenile orchards (4% of larvae). A significantly higher occurrence of EPF was recorded in non-bearing and mature orchards, with 40% and 37% occurrence respectively, than in juvenile orchards, with 25% occurrence recorded. EPF occurrence in juvenile orchards increased significantly by 16% to 32% from the first to the third year of sampling. In contrast to results recorded in laboratory trials, similar or higher pest pressure in juvenile orchards than mature orchards did not always result in significantly higher levels of FCM damage under field conditions. FCM damage in juvenile orchards may have been lower than expected, as greater extremes of temperature and lower humidity were recorded in juvenile orchards, which would increase larval mortality. Results of this study showed that juvenile and mature orchards are significantly different and should be managed differently.
- Full Text:
- Date Issued: 2018
- Authors: Albertyn, Sonnica
- Date: 2018
- Subjects: Cryptophlebia leucotreta , Population biology , Insect populations , Orchards , Insect nematodes , Entomopathogenic fungi
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62615 , vital:28213
- Description: Anecdotal reports in the South African citrus industry claim higher populations of false codling moth (FCM), Thaumatotibia (Cryptophlebia) leucotreta (Meyr) (Lepidoptera: Tortricidae), in orchards during the first three to five harvesting years of citrus planted in virgin soil, after which, FCM numbers seem to decrease and remain consistent. Various laboratory studies and field surveys were conducted to determine if, and why juvenile orchards (four to eight years old) experience higher FCM infestation than mature orchards (nine years and older). In laboratory trials, Washington Navel oranges and Nova Mandarins from juvenile trees were shown to be significantly more susceptible to FCM damage and significantly more attractive for oviposition in both choice and no-choice trials, than fruit from mature trees. Although fruit from juvenile Cambria Navel trees were significantly more attractive than mature orchards for oviposition, they were not more susceptible to FCM damage. In contrast, fruit from juvenile and mature Midnight Valencia orchards were equally attractive for oviposition, but fruit from juvenile trees were significantly more susceptible to FCM damage than fruit from mature trees. Artificial diets were augmented with powder from fruit from juvenile or mature Washington Navel orchards at 5%, 10%, 15% or 30%. Higher larval survival of 76%, 63%, 50% and 34%, respectively, was recorded on diets containing fruit powder from the juvenile trees than on diets containing fruit powder from the mature trees, at 69%, 57%, 44% and 27% larval survival, respectively. Bioassays were conducted to determine if differences in plant chemistry between fruit from juvenile and mature trees will have an impact on the susceptibility FCM to entomopathogenic nematodes (EPN), entomopathogenic fungi (EPF) and Cryptophlebia leucotreta granulovirus (CrleGV). No significant differences in the susceptibility of larvae reared on diets containing 15% fruit powder from juvenile and mature trees to EPN and EPF were recorded. Mortality of neonate larvae was significantly lower when placed on diets containing 15% fruit powder from mature trees (45% mortality) than diets containing 15% fruit powder from juvenile trees (61% mortality), after larvae ingested the lowest virus concentration tested, being 2 x104 OBs/ml. Data collected from field surveys showed significantly lower egg parasitism, virus infection of larvae and EPF occurrence in juvenile orchards than mature orchards. Egg parasitism was between 11% and 54% higher in mature orchards than juvenile orchards, with the exception of Mandarins during 2015, where egg parasitism was slightly higher in juvenile orchards, but not significantly so. A significantly higher proportion of larvae retrieved from mature orchards (7% of larvae) were infected with CrleGV than larvae retrieved from juvenile orchards (4% of larvae). A significantly higher occurrence of EPF was recorded in non-bearing and mature orchards, with 40% and 37% occurrence respectively, than in juvenile orchards, with 25% occurrence recorded. EPF occurrence in juvenile orchards increased significantly by 16% to 32% from the first to the third year of sampling. In contrast to results recorded in laboratory trials, similar or higher pest pressure in juvenile orchards than mature orchards did not always result in significantly higher levels of FCM damage under field conditions. FCM damage in juvenile orchards may have been lower than expected, as greater extremes of temperature and lower humidity were recorded in juvenile orchards, which would increase larval mortality. Results of this study showed that juvenile and mature orchards are significantly different and should be managed differently.
- Full Text:
- Date Issued: 2018
The intersection between social development and the internationalisation of higher education in Colombia
- Authors: Anzola-Pardo, Giovanni
- Date: 2018
- Subjects: Education, Higher -- Colombia , Education and globalization International education -- Colombia Education, Higher -- International cooperation -- Colombia
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/23371 , vital:30536
- Description: The study addresses the nature of Colombian sustainable social development and the internationalisation of higher education (iHE). This intersection is analysed within various theoretical paradigms surrounding social sustainable development and the internationalisation of higher education. This study aims at reviewing the activities of Higher Education Institutions (HEIs) which focus on internationalisation. The analysis focuses on the manner in which HEIs in Colombia approach internationalisation vis-à-vis social sustainable development, and emphasis is made on knowledge transfer, research and community engagement. Using the theories of sustainable development, the iHE, in terms of practises and policies, are assessed. Within a mixed-methods approach, data gathered from Colombian university heads (16 presidents and vice-presidents) and 133 individuals responsible for internationalisation affairs is analysed. Concurrent triangulation is used to help introduce a series of themes and sub-themes derived from the qualitative and quantitative information. Within a social constructionist framework, this study has a twofold purpose. On the one hand, it is sought to identify and understand the challenges for Colombia’s academic internationalisation; on the other, an in-depth approach is presented in relation to the way different stakeholders perceive the society-HE internationalisation nexus. The major findings of the study indicate that there is a need to clarify and revise Colombia’s Higher Education policy to address both the education needs of the society and its social development requirements. It was also found that higher education internationalisation could act as an important agent of social change.
- Full Text:
- Date Issued: 2018
- Authors: Anzola-Pardo, Giovanni
- Date: 2018
- Subjects: Education, Higher -- Colombia , Education and globalization International education -- Colombia Education, Higher -- International cooperation -- Colombia
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/23371 , vital:30536
- Description: The study addresses the nature of Colombian sustainable social development and the internationalisation of higher education (iHE). This intersection is analysed within various theoretical paradigms surrounding social sustainable development and the internationalisation of higher education. This study aims at reviewing the activities of Higher Education Institutions (HEIs) which focus on internationalisation. The analysis focuses on the manner in which HEIs in Colombia approach internationalisation vis-à-vis social sustainable development, and emphasis is made on knowledge transfer, research and community engagement. Using the theories of sustainable development, the iHE, in terms of practises and policies, are assessed. Within a mixed-methods approach, data gathered from Colombian university heads (16 presidents and vice-presidents) and 133 individuals responsible for internationalisation affairs is analysed. Concurrent triangulation is used to help introduce a series of themes and sub-themes derived from the qualitative and quantitative information. Within a social constructionist framework, this study has a twofold purpose. On the one hand, it is sought to identify and understand the challenges for Colombia’s academic internationalisation; on the other, an in-depth approach is presented in relation to the way different stakeholders perceive the society-HE internationalisation nexus. The major findings of the study indicate that there is a need to clarify and revise Colombia’s Higher Education policy to address both the education needs of the society and its social development requirements. It was also found that higher education internationalisation could act as an important agent of social change.
- Full Text:
- Date Issued: 2018
An investigation into the introduction of a new wealth tax in South Africa
- Authors: Arendse, Jacqueline A
- Date: 2018
- Subjects: Wealth tax -- Law and legislation -- South Africa , Taxation -- Law and legislation -- South Africa , Income tax -- South Africa , Fiscal policy -- South Africa , South Africa -- Economic conditions , Income distribution -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61379 , vital:28020
- Description: In a world of economic uncertainty and manifold social problems, South Africa has its own unique challenges of low economic growth, persistent budget deficits that produce increasing government debt and the highest level of economic inequality in the world. The history of injustice and economic marginalisation and the failure of the economy to provide inclusive growth drives an urgent need to address economic inequality through tax policy, placing ever more focus on wealth taxes as a possible solution. There is a hope is that taxing the wealthy may provide the opportunity to redistribute desperately-needed resources to those denied the opportunity to build wealth and who are trapped in the cycle of poverty. Yet, as appealing as a new wealth tax may seem, the introduction of such a tax carries with it a range of risks, not all of which are known. Of great concern is the possible effect on the economy, which, in its vulnerable state, cannot afford any loss of capital and investment. Very little research has been done on wealth tax in the South African context and there is a dearth of literature focusing on the views and perceptions of the wealthy individuals themselves. This qualitative study investigates the merits and disadvantages of a new wealth tax and seeks to identify any unintended consequences that could result from the implementation of a new wealth tax in South Africa, drawing from historical and international experience and primary data obtained from interviews with individuals likely to be affected by such a tax. Having explored the literature and international experiences with wealth tax and having probed the thinking of wealthy individuals who would be the payers of a wealth tax, the study finds that a new wealth tax may contribute towards the progressivity of the tax system, but it is doubtful whether such a tax would provide a sustainable revenue stream that would be sufficient to address economic inequality and there is a risk of causing harm to the economy. Recognising that the motivation for wealth taxes is often driven more by political argument and public perception than by rational quantitative analysis, the study also anticipates the introduction of a new wealth tax and suggests guidelines for the design of such a tax within the framework for evaluating a good tax system. This study informs the debate on wealth taxes in South Africa and contributes to the design of such a tax, should it be implemented.
- Full Text:
- Date Issued: 2018
- Authors: Arendse, Jacqueline A
- Date: 2018
- Subjects: Wealth tax -- Law and legislation -- South Africa , Taxation -- Law and legislation -- South Africa , Income tax -- South Africa , Fiscal policy -- South Africa , South Africa -- Economic conditions , Income distribution -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61379 , vital:28020
- Description: In a world of economic uncertainty and manifold social problems, South Africa has its own unique challenges of low economic growth, persistent budget deficits that produce increasing government debt and the highest level of economic inequality in the world. The history of injustice and economic marginalisation and the failure of the economy to provide inclusive growth drives an urgent need to address economic inequality through tax policy, placing ever more focus on wealth taxes as a possible solution. There is a hope is that taxing the wealthy may provide the opportunity to redistribute desperately-needed resources to those denied the opportunity to build wealth and who are trapped in the cycle of poverty. Yet, as appealing as a new wealth tax may seem, the introduction of such a tax carries with it a range of risks, not all of which are known. Of great concern is the possible effect on the economy, which, in its vulnerable state, cannot afford any loss of capital and investment. Very little research has been done on wealth tax in the South African context and there is a dearth of literature focusing on the views and perceptions of the wealthy individuals themselves. This qualitative study investigates the merits and disadvantages of a new wealth tax and seeks to identify any unintended consequences that could result from the implementation of a new wealth tax in South Africa, drawing from historical and international experience and primary data obtained from interviews with individuals likely to be affected by such a tax. Having explored the literature and international experiences with wealth tax and having probed the thinking of wealthy individuals who would be the payers of a wealth tax, the study finds that a new wealth tax may contribute towards the progressivity of the tax system, but it is doubtful whether such a tax would provide a sustainable revenue stream that would be sufficient to address economic inequality and there is a risk of causing harm to the economy. Recognising that the motivation for wealth taxes is often driven more by political argument and public perception than by rational quantitative analysis, the study also anticipates the introduction of a new wealth tax and suggests guidelines for the design of such a tax within the framework for evaluating a good tax system. This study informs the debate on wealth taxes in South Africa and contributes to the design of such a tax, should it be implemented.
- Full Text:
- Date Issued: 2018
Mannich base metal complexes and their thiocyanate analogues as catalysts in the oxidation of Catechol
- Authors: Ayeni, Ayowole Olaolu
- Date: 2018
- Subjects: Mannich bases , Catechol , Catechol -- Oxidation , Thiocyanates , Catalysts
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62339 , vital:28156
- Description: The study focused on the design of new Cu(II) and Fe(III) complexes, with or without thiocyanate (NCS-), as possible candidates of catechol oxidation using 3,5-di-tert-butyl catechol (3,5-DTBC) as substrate. Two classes of Mannich bases were studied depending on the active methylene group from which they were formed, being either p-cresol or acetaminophen. The ligands were characterised by 1H and 13C NMR spectroscopy. Crystal structures of three of the ligands are newly reported, along with detailed discussion of polymorphism observed in one of the ligands, and the nature of the hydrogen within the ligands in the solid state as well as in solution. The Mannich bases behaved as bidentate (NO), tridentate (NNO) and tetradentate (NNOO) ligands on coordination to Cu(II) and Fe(III) ions in which the hydroxyl group may be protonated or deprotonated. Coordination was determined by IR spectroscopy, investigating shifts in vOH, vC-O and in vCNC of the Mannich bases. The vCNC stretching frequencies v1 and v2 of asymmetrical piperazine Mannich bases were observed to shift upward in few cases upon complexation and this is attributed to (chair-boat) conformational change. The mode of coordination of the thiocyanate was determined by IR spectroscopy. Of the forty metal complexes investigated, six groups of metal complexes were identified as follows: (i) Ma(Ln)aClb-cH2O; (ii) Ma(HLn)a(NCS)aClb; (iii) Ma(Ln)a(NCS)aClb; (iv) Ma(HLn)aClb-cH2O; (v) Ma(Ln)a(NCS)a-cH2O; (vi) Ma(HLn)a(NCS)a-cH2O where a = 1 - 2 ; b = 1 - 4, c = 1 - 8. Molar conductivity values of 4.38 - 161.77 Q-1.cm2.mol-1 for the Cu(II) and Fe(III) complexes in DMSO showed that they range from non-electrolytes to 1:1 and 1:2 electrolytes. Electronic spectra for the ligands and the complexes were conducted in DMF and DMSO. The ligands are characterised by and n→n* and n→n* transitions. Intraligand charge transfer transitions peculiar to the nitro group were observed at about 430 nm for the nitro containing ligands. On coordination, these bands overshadowed the d-d transitions particularly for the nitro-Mannich bases. On complexation, ligand to metal charge transfer transitions associated with the hydroxyl were observed between 320 - 420 nm. Charge transfer transitions associated with the thiocyanates were also observed and discussed. The d-d transitions for high spin Fe(III) complexes are spin forbidden and generally uninformative. Those of Cu(II) are spin allowed and allow tentative structural proposals. Square planar and octahedral geometry are generally prevalent in the Cu(II) complexes with trigonal bipyramidal observed in few instances. The Fe(III) complexes are generally octahedral. Thirty-nine of the forty synthesised Cu(II) and Fe(III) complexes were catalytically active on the substrate (3,5-DTBC) in DMF with turnover rates (kcat) reported in the range of 1.86 ± 0.09 to 112.32 ± 3.72 h-1. From this pool of complexes, sixteen isostructural pairs were identified in terms of geometry, molecular formula and the source of the Mannich base and the following conclusions were made: The presence of thiocyanate in the metal complexes reduce catecholase activity; the Cu(II) complexes generally have better activity but the Fe(III) complexes become more relatively active with highly electron donating groups while the Cu(II) complexes become less; dinuclear complexes have greater activity than the mononuclear.
- Full Text:
- Date Issued: 2018
- Authors: Ayeni, Ayowole Olaolu
- Date: 2018
- Subjects: Mannich bases , Catechol , Catechol -- Oxidation , Thiocyanates , Catalysts
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62339 , vital:28156
- Description: The study focused on the design of new Cu(II) and Fe(III) complexes, with or without thiocyanate (NCS-), as possible candidates of catechol oxidation using 3,5-di-tert-butyl catechol (3,5-DTBC) as substrate. Two classes of Mannich bases were studied depending on the active methylene group from which they were formed, being either p-cresol or acetaminophen. The ligands were characterised by 1H and 13C NMR spectroscopy. Crystal structures of three of the ligands are newly reported, along with detailed discussion of polymorphism observed in one of the ligands, and the nature of the hydrogen within the ligands in the solid state as well as in solution. The Mannich bases behaved as bidentate (NO), tridentate (NNO) and tetradentate (NNOO) ligands on coordination to Cu(II) and Fe(III) ions in which the hydroxyl group may be protonated or deprotonated. Coordination was determined by IR spectroscopy, investigating shifts in vOH, vC-O and in vCNC of the Mannich bases. The vCNC stretching frequencies v1 and v2 of asymmetrical piperazine Mannich bases were observed to shift upward in few cases upon complexation and this is attributed to (chair-boat) conformational change. The mode of coordination of the thiocyanate was determined by IR spectroscopy. Of the forty metal complexes investigated, six groups of metal complexes were identified as follows: (i) Ma(Ln)aClb-cH2O; (ii) Ma(HLn)a(NCS)aClb; (iii) Ma(Ln)a(NCS)aClb; (iv) Ma(HLn)aClb-cH2O; (v) Ma(Ln)a(NCS)a-cH2O; (vi) Ma(HLn)a(NCS)a-cH2O where a = 1 - 2 ; b = 1 - 4, c = 1 - 8. Molar conductivity values of 4.38 - 161.77 Q-1.cm2.mol-1 for the Cu(II) and Fe(III) complexes in DMSO showed that they range from non-electrolytes to 1:1 and 1:2 electrolytes. Electronic spectra for the ligands and the complexes were conducted in DMF and DMSO. The ligands are characterised by and n→n* and n→n* transitions. Intraligand charge transfer transitions peculiar to the nitro group were observed at about 430 nm for the nitro containing ligands. On coordination, these bands overshadowed the d-d transitions particularly for the nitro-Mannich bases. On complexation, ligand to metal charge transfer transitions associated with the hydroxyl were observed between 320 - 420 nm. Charge transfer transitions associated with the thiocyanates were also observed and discussed. The d-d transitions for high spin Fe(III) complexes are spin forbidden and generally uninformative. Those of Cu(II) are spin allowed and allow tentative structural proposals. Square planar and octahedral geometry are generally prevalent in the Cu(II) complexes with trigonal bipyramidal observed in few instances. The Fe(III) complexes are generally octahedral. Thirty-nine of the forty synthesised Cu(II) and Fe(III) complexes were catalytically active on the substrate (3,5-DTBC) in DMF with turnover rates (kcat) reported in the range of 1.86 ± 0.09 to 112.32 ± 3.72 h-1. From this pool of complexes, sixteen isostructural pairs were identified in terms of geometry, molecular formula and the source of the Mannich base and the following conclusions were made: The presence of thiocyanate in the metal complexes reduce catecholase activity; the Cu(II) complexes generally have better activity but the Fe(III) complexes become more relatively active with highly electron donating groups while the Cu(II) complexes become less; dinuclear complexes have greater activity than the mononuclear.
- Full Text:
- Date Issued: 2018
Servant leadership: antecedent to Quality of Worklife of customer service frontline employees
- Authors: Bedser, Mark Bernard
- Date: 2018
- Subjects: Call center agents Servant leadership Customer Service -- Quality control Employee morale Job satisfaction Quality of work life
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62144 , vital:28132
- Description: Contact Centre agents operate in closely monitored and highly controlled environments and their work consists of solving service requests or assisting customers with information on products or services. Consequently their work involves a great deal of emotional labour and stress. It is not surprising then, that the working environment of the Contact Centre is reported to have a negative impact on the levels of Quality of Worklife of Contact Centre agents, and that in the Contact Centre context, it is likely that low levels of Quality of Worklife exist. It is argued that it is important for organisations to be particularly aware of the Quality of Worklife perceptions of their employees should they want to address Quality of Worklife levels and benefit from the positive consequences of higher levels of the construct. Numerous variables are reported to play either an antecedent, moderating, mediating, or consequential role in relation to the Quality of Worklife construct. A systems model of Quality of Worklife is developed, which illustrates the inter-relationships of these variables and how they affect and are affected by the Quality of Worklife construct. It is argued that leadership is an important antecedent to Quality of Worklife, and this is the antecedent of interest in this study. It is proposed that it is not just any leadership that will contribute to an improved Quality of Worklife, particularly within a challenging context such as the Contact Centre environment. Rather, it is suggested that certain qualities of leaders will have a greater influence on Quality of Worklife. For example, leaders who focus on relationships and are caring - characteristics associated with servant leaders - are deemed more suitable for the Contact Centre context. The research also proposes that there are close associations between Servant Leadership and Trust, which in turn has the potential to affect Quality of Worklife positively. It is argued, therefore, that Trust mediates the relationship between Servant Leadership and Quality of Worklife in the customer service frontline context. While there is a broad base of literature available on servant leadership that focuses on the senior or executive level of leadership, Van Dierendonck and Nuijten (2011) have argued that it is also relevant at the middle level of management and have validated an eight dimensional measure of servant leadership that is suitable for this management level. The Van Laar, Edwards and Easton (2007) Quality of Worklife model is also argued to be an appropriate model and measure of the Quality of Worklife construct, due to the robustness of the instrument design and the appropriateness of its underlying theory to the context of this research. Research has shown that leadership can have a significant relationship with Quality of Worklife. Moreover, a review of the literature on servant leadership reveals that trust, satisfaction, general well-being, and commitment to their jobs increases when employees are exposed to leadership behaviours associated with servant leadership. There is however, no evidence in the literature of any investigation of the relationship between Servant Leadership and Quality of Worklife, or of research investigating the partial mediating effects of Trust between these two constructs. Research was conducted to test this relationship. A survey questionnaire was administered amongst a sample of 555 Contact Centre agents, who were employed in eight different organisations. Confirmatory factor analysis procedures were conducted in STATA (V15.0), to test and validate the factor structure of Servant Leadership and Quality of Worklife models. The research also produced a Servant Leadership, Trust and Quality of Worklife structural equation model that supported the hypotheses of the relationships between the constructs. Mediation analysis confirmed Trust’s role as a mediator between Servant Leadership and Quality of Worklife. The structural equation model confirmed that synergies between Servant Leadership, Trust and Quality of Worklife exist, and that Trust partially mediates the relationship between Servant Leadership and Quality of Worklife. It is therefore argued that an increase in Servant Leadership behaviour by the manager or supervisor of frontline staff has a positive association with increases of Trust, as well as positive associations with Quality of Worklife experienced by employees in the frontline context. Moreover, it is also posited that the relationship between Servant Leadership and Quality of Worklife is partially mediated by Trust of the supervisor. The implications of these results are discussed, and recommendations made for management practice and further research.
- Full Text:
- Date Issued: 2018
- Authors: Bedser, Mark Bernard
- Date: 2018
- Subjects: Call center agents Servant leadership Customer Service -- Quality control Employee morale Job satisfaction Quality of work life
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62144 , vital:28132
- Description: Contact Centre agents operate in closely monitored and highly controlled environments and their work consists of solving service requests or assisting customers with information on products or services. Consequently their work involves a great deal of emotional labour and stress. It is not surprising then, that the working environment of the Contact Centre is reported to have a negative impact on the levels of Quality of Worklife of Contact Centre agents, and that in the Contact Centre context, it is likely that low levels of Quality of Worklife exist. It is argued that it is important for organisations to be particularly aware of the Quality of Worklife perceptions of their employees should they want to address Quality of Worklife levels and benefit from the positive consequences of higher levels of the construct. Numerous variables are reported to play either an antecedent, moderating, mediating, or consequential role in relation to the Quality of Worklife construct. A systems model of Quality of Worklife is developed, which illustrates the inter-relationships of these variables and how they affect and are affected by the Quality of Worklife construct. It is argued that leadership is an important antecedent to Quality of Worklife, and this is the antecedent of interest in this study. It is proposed that it is not just any leadership that will contribute to an improved Quality of Worklife, particularly within a challenging context such as the Contact Centre environment. Rather, it is suggested that certain qualities of leaders will have a greater influence on Quality of Worklife. For example, leaders who focus on relationships and are caring - characteristics associated with servant leaders - are deemed more suitable for the Contact Centre context. The research also proposes that there are close associations between Servant Leadership and Trust, which in turn has the potential to affect Quality of Worklife positively. It is argued, therefore, that Trust mediates the relationship between Servant Leadership and Quality of Worklife in the customer service frontline context. While there is a broad base of literature available on servant leadership that focuses on the senior or executive level of leadership, Van Dierendonck and Nuijten (2011) have argued that it is also relevant at the middle level of management and have validated an eight dimensional measure of servant leadership that is suitable for this management level. The Van Laar, Edwards and Easton (2007) Quality of Worklife model is also argued to be an appropriate model and measure of the Quality of Worklife construct, due to the robustness of the instrument design and the appropriateness of its underlying theory to the context of this research. Research has shown that leadership can have a significant relationship with Quality of Worklife. Moreover, a review of the literature on servant leadership reveals that trust, satisfaction, general well-being, and commitment to their jobs increases when employees are exposed to leadership behaviours associated with servant leadership. There is however, no evidence in the literature of any investigation of the relationship between Servant Leadership and Quality of Worklife, or of research investigating the partial mediating effects of Trust between these two constructs. Research was conducted to test this relationship. A survey questionnaire was administered amongst a sample of 555 Contact Centre agents, who were employed in eight different organisations. Confirmatory factor analysis procedures were conducted in STATA (V15.0), to test and validate the factor structure of Servant Leadership and Quality of Worklife models. The research also produced a Servant Leadership, Trust and Quality of Worklife structural equation model that supported the hypotheses of the relationships between the constructs. Mediation analysis confirmed Trust’s role as a mediator between Servant Leadership and Quality of Worklife. The structural equation model confirmed that synergies between Servant Leadership, Trust and Quality of Worklife exist, and that Trust partially mediates the relationship between Servant Leadership and Quality of Worklife. It is therefore argued that an increase in Servant Leadership behaviour by the manager or supervisor of frontline staff has a positive association with increases of Trust, as well as positive associations with Quality of Worklife experienced by employees in the frontline context. Moreover, it is also posited that the relationship between Servant Leadership and Quality of Worklife is partially mediated by Trust of the supervisor. The implications of these results are discussed, and recommendations made for management practice and further research.
- Full Text:
- Date Issued: 2018
Biotic and abiotic drivers of macroinvertebrate assemblages in a South African river
- Authors: Bellingan, Terence Andrew
- Date: 2018
- Subjects: Aquatic insects -- South Africa -- Eastern Cape , Stream ecology -- South Africa -- Eastern Cape , Freshwater ecology -- South Africa -- Eastern Cape , Riparian areas -- Management , Ecosystem management -- South Africa -- Eastern Cape , Mayflies -- South Africa -- Eastern Cape , Stoneflies -- South Africa -- Eastern Cape , Keiskamma River
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61839 , vital:28067
- Description: Aquatic insects are the most numerically abundant and diverse group of organisms found in lotic ecosystems in South Africa and the world over. They play vital roles in freshwater ecosystem functioning, processing nutrients and in turn forming integral links in stream food-webs. This thesis focussed on examining the macroinvertebrate fauna within three reaches of headwater streams of the Keiskamma River system: reaches that were considered to be fishless; reaches that were invaded by non-native salmonid species; and reaches that were dominated by native fish. I described the effects of predatory fish presence through detailed examination of macroinvertebrate assemblage composition; macroinvertebrate drift timing and density; and through niche utilisation determined from stable isotope data. Patterns in the macroinvertebrate assemblages of the headwaters of the Keiskamma River appear to be driven more strongly by flow rate and seasonal influences, but fish presence and biotope availability were also significant drivers. Niche shifts due to predator presence were not easy to detect and, while patterns of influence may have been evident, they were not found to be significant. However, I demonstrated that salmonids selectively feed on native fish species when the opportunity is presented, occupying significantly higher trophic levels when co-occurring with native fish than in invaded reaches where native fish are absent. Drift timing and density were demonstrated to be significantly different between reach for specific macroinvertebrate species from the Ephemeroptera and Plecoptera, under differing fish predation regimes, in agreement with what has been observed from studies in rivers elsewhere. In freshwater ecosystems of South Africa and worldwide, mitigation of negative effects of alien fishes through their removal using piscicides may also affect non-target organisms. To better understand the effects of just such a removal operation, employed for the first time in the history of freshwater conservation in South Africa, macroinvertebrate communities were assessed for non-target effects of rotenone. The fish eradication operations were demonstrated to have a short-term negative effect on the macroinvertebrate assemblage, through water quality index measurements and alteration of densities of macroinvertebrate taxa collected from stone surfaces. However, no long-term detrimental impact was observed as macroinvertebrate faunas returned to a comparable pre-treatment state within a year of each rotenone application.
- Full Text:
- Date Issued: 2018
- Authors: Bellingan, Terence Andrew
- Date: 2018
- Subjects: Aquatic insects -- South Africa -- Eastern Cape , Stream ecology -- South Africa -- Eastern Cape , Freshwater ecology -- South Africa -- Eastern Cape , Riparian areas -- Management , Ecosystem management -- South Africa -- Eastern Cape , Mayflies -- South Africa -- Eastern Cape , Stoneflies -- South Africa -- Eastern Cape , Keiskamma River
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61839 , vital:28067
- Description: Aquatic insects are the most numerically abundant and diverse group of organisms found in lotic ecosystems in South Africa and the world over. They play vital roles in freshwater ecosystem functioning, processing nutrients and in turn forming integral links in stream food-webs. This thesis focussed on examining the macroinvertebrate fauna within three reaches of headwater streams of the Keiskamma River system: reaches that were considered to be fishless; reaches that were invaded by non-native salmonid species; and reaches that were dominated by native fish. I described the effects of predatory fish presence through detailed examination of macroinvertebrate assemblage composition; macroinvertebrate drift timing and density; and through niche utilisation determined from stable isotope data. Patterns in the macroinvertebrate assemblages of the headwaters of the Keiskamma River appear to be driven more strongly by flow rate and seasonal influences, but fish presence and biotope availability were also significant drivers. Niche shifts due to predator presence were not easy to detect and, while patterns of influence may have been evident, they were not found to be significant. However, I demonstrated that salmonids selectively feed on native fish species when the opportunity is presented, occupying significantly higher trophic levels when co-occurring with native fish than in invaded reaches where native fish are absent. Drift timing and density were demonstrated to be significantly different between reach for specific macroinvertebrate species from the Ephemeroptera and Plecoptera, under differing fish predation regimes, in agreement with what has been observed from studies in rivers elsewhere. In freshwater ecosystems of South Africa and worldwide, mitigation of negative effects of alien fishes through their removal using piscicides may also affect non-target organisms. To better understand the effects of just such a removal operation, employed for the first time in the history of freshwater conservation in South Africa, macroinvertebrate communities were assessed for non-target effects of rotenone. The fish eradication operations were demonstrated to have a short-term negative effect on the macroinvertebrate assemblage, through water quality index measurements and alteration of densities of macroinvertebrate taxa collected from stone surfaces. However, no long-term detrimental impact was observed as macroinvertebrate faunas returned to a comparable pre-treatment state within a year of each rotenone application.
- Full Text:
- Date Issued: 2018
Biochemical characterisation and small molecule modulation of the interaction between two cytosolic Hsp70s from Trypanosoma brucei and potential co-chaperones
- Authors: Bentley, Stephen John
- Date: 2018
- Subjects: Uncatalogued
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63402 , vital:28407
- Description: Expected release date-April 2019
- Full Text:
- Date Issued: 2018
- Authors: Bentley, Stephen John
- Date: 2018
- Subjects: Uncatalogued
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63402 , vital:28407
- Description: Expected release date-April 2019
- Full Text:
- Date Issued: 2018
Implementation of programmes for maintaining care and safety of learners in secondary schools in the Graaff Reinet education district
- Authors: Bilatyi, Nkosana Carlon
- Date: 2018
- Subjects: Schools -- Security measures -- Eastern Cape School environment -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9379 , vital:34339
- Description: Care and safety of learners refers to the behavioral practices which protect children from danger, risks and injuries. It also includes environment that is free from violence since where there is no fear perceived, there is reverence for the school and its disciplinary measures (Hernandez & Seem, 2004; Marotz, Cross, Rush, 2005; Mathe, 2008; Chukwu, 2008). Schools should have positive environment which allows members of staff, learners and guests to freely interact without threats or fear. Learners and staff should feel psychologically, physically and emotionally safe and secure (Dwyer & Osher, 2000; Tabancali &Bektas, 2009; Butcher& Manning 2005). Consequently, care and safety of learners can also be viewed in a more practical and management oriented way. According to Hull (2010) care and safety of learners in a management oriented way includes, maintenance of school buildings and grounds, care of facilities, furniture and equipment with spacious corridors that allow free flow and emergency movement to prevent illness and death. Classes should accommodate the required educator-learner ratio. The playgrounds are reduced to limited space to ensure that learners are visible and are controllable. Moreover provision of care and safety of learners includes school culture and the appropriate training of educators as well as school staff who should respond to threats and hazards. It also includes addressing the range of crisis the school can face such as death and natural disasters (Phaneuf, 2009). Reeves, Nickerson, Conolly-Wilson, Suson, Lazzaro, Jimerson, and Pesce (2012) contend that the educators on duty should provide learners with safety precautions to guard against the possibility of single learner slipping away and suffering injuries (Dilion, 2007). According to Mogopodi, Banyaladzi & Petros (2015) facilities like laboratories should be suitable for all learners because they help to transform theoretical education into practice making learning effective. Astor, Guerra, Van Acker (2010) suggest that provision of care and safety for learners is among the most essential components for achieving effective teaching and learning. This will only be achieved by putting in place proper care and safety programmes and ensuring that they are effectively implemented (Netshitahame, & Vollenhoven, 2002, Astor et.al.2010).
- Full Text:
- Date Issued: 2018
- Authors: Bilatyi, Nkosana Carlon
- Date: 2018
- Subjects: Schools -- Security measures -- Eastern Cape School environment -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9379 , vital:34339
- Description: Care and safety of learners refers to the behavioral practices which protect children from danger, risks and injuries. It also includes environment that is free from violence since where there is no fear perceived, there is reverence for the school and its disciplinary measures (Hernandez & Seem, 2004; Marotz, Cross, Rush, 2005; Mathe, 2008; Chukwu, 2008). Schools should have positive environment which allows members of staff, learners and guests to freely interact without threats or fear. Learners and staff should feel psychologically, physically and emotionally safe and secure (Dwyer & Osher, 2000; Tabancali &Bektas, 2009; Butcher& Manning 2005). Consequently, care and safety of learners can also be viewed in a more practical and management oriented way. According to Hull (2010) care and safety of learners in a management oriented way includes, maintenance of school buildings and grounds, care of facilities, furniture and equipment with spacious corridors that allow free flow and emergency movement to prevent illness and death. Classes should accommodate the required educator-learner ratio. The playgrounds are reduced to limited space to ensure that learners are visible and are controllable. Moreover provision of care and safety of learners includes school culture and the appropriate training of educators as well as school staff who should respond to threats and hazards. It also includes addressing the range of crisis the school can face such as death and natural disasters (Phaneuf, 2009). Reeves, Nickerson, Conolly-Wilson, Suson, Lazzaro, Jimerson, and Pesce (2012) contend that the educators on duty should provide learners with safety precautions to guard against the possibility of single learner slipping away and suffering injuries (Dilion, 2007). According to Mogopodi, Banyaladzi & Petros (2015) facilities like laboratories should be suitable for all learners because they help to transform theoretical education into practice making learning effective. Astor, Guerra, Van Acker (2010) suggest that provision of care and safety for learners is among the most essential components for achieving effective teaching and learning. This will only be achieved by putting in place proper care and safety programmes and ensuring that they are effectively implemented (Netshitahame, & Vollenhoven, 2002, Astor et.al.2010).
- Full Text:
- Date Issued: 2018
Investigating combinations of feature extraction and classification for improved image-based multimodal biometric systems at the feature level
- Authors: Brown, Dane
- Date: 2018
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63470 , vital:28414
- Description: Multimodal biometrics has become a popular means of overcoming the limitations of unimodal biometric systems. However, the rich information particular to the feature level is of a complex nature and leveraging its potential without overfitting a classifier is not well studied. This research investigates feature-classifier combinations on the fingerprint, face, palmprint, and iris modalities to effectively fuse their feature vectors for a complementary result. The effects of different feature-classifier combinations are thus isolated to identify novel or improved algorithms. A new face segmentation algorithm is shown to increase consistency in nominal and extreme scenarios. Moreover, two novel feature extraction techniques demonstrate better adaptation to dynamic lighting conditions, while reducing feature dimensionality to the benefit of classifiers. A comprehensive set of unimodal experiments are carried out to evaluate both verification and identification performance on a variety of datasets using four classifiers, namely Eigen, Fisher, Local Binary Pattern Histogram and linear Support Vector Machine on various feature extraction methods. The recognition performance of the proposed algorithms are shown to outperform the vast majority of related studies, when using the same dataset under the same test conditions. In the unimodal comparisons presented, the proposed approaches outperform existing systems even when given a handicap such as fewer training samples or data with a greater number of classes. A separate comprehensive set of experiments on feature fusion show that combining modality data provides a substantial increase in accuracy, with only a few exceptions that occur when differences in the image data quality of two modalities are substantial. However, when two poor quality datasets are fused, noticeable gains in recognition performance are realized when using the novel feature extraction approach. Finally, feature-fusion guidelines are proposed to provide the necessary insight to leverage the rich information effectively when fusing multiple biometric modalities at the feature level. These guidelines serve as the foundation to better understand and construct biometric systems that are effective in a variety of applications.
- Full Text:
- Date Issued: 2018
- Authors: Brown, Dane
- Date: 2018
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63470 , vital:28414
- Description: Multimodal biometrics has become a popular means of overcoming the limitations of unimodal biometric systems. However, the rich information particular to the feature level is of a complex nature and leveraging its potential without overfitting a classifier is not well studied. This research investigates feature-classifier combinations on the fingerprint, face, palmprint, and iris modalities to effectively fuse their feature vectors for a complementary result. The effects of different feature-classifier combinations are thus isolated to identify novel or improved algorithms. A new face segmentation algorithm is shown to increase consistency in nominal and extreme scenarios. Moreover, two novel feature extraction techniques demonstrate better adaptation to dynamic lighting conditions, while reducing feature dimensionality to the benefit of classifiers. A comprehensive set of unimodal experiments are carried out to evaluate both verification and identification performance on a variety of datasets using four classifiers, namely Eigen, Fisher, Local Binary Pattern Histogram and linear Support Vector Machine on various feature extraction methods. The recognition performance of the proposed algorithms are shown to outperform the vast majority of related studies, when using the same dataset under the same test conditions. In the unimodal comparisons presented, the proposed approaches outperform existing systems even when given a handicap such as fewer training samples or data with a greater number of classes. A separate comprehensive set of experiments on feature fusion show that combining modality data provides a substantial increase in accuracy, with only a few exceptions that occur when differences in the image data quality of two modalities are substantial. However, when two poor quality datasets are fused, noticeable gains in recognition performance are realized when using the novel feature extraction approach. Finally, feature-fusion guidelines are proposed to provide the necessary insight to leverage the rich information effectively when fusing multiple biometric modalities at the feature level. These guidelines serve as the foundation to better understand and construct biometric systems that are effective in a variety of applications.
- Full Text:
- Date Issued: 2018
Bioinformatics tool development with a focus on structural bioinformatics and the analysis of genetic variation in humans
- Authors: Brown, David K
- Date: 2018
- Subjects: Bioinformatics , Human genetics -- Variation , High performance computing , Workflow management systems , Molecular dynamics , Next generation sequencing , Human Mutation Analysis (HUMA)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/60708 , vital:27820
- Description: This thesis is divided into three parts, united under the general theme of bioinformatics tool development and variation analysis. Part 1 describes the design and development of the Job Management System (JMS), a workflow management system for high performance computing (HPC). HPC has become an integral part of bioinformatics. Computational methods for molecular dynamics and next generation sequencing (NGS) analysis, which require complex calculations on large datasets, are not yet feasible on desktop computers. As such, powerful computer clusters have been employed to perform these calculations. However, making use of these HPC clusters requires familiarity with command line interfaces. This excludes a large number of researchers from taking advantage of these resources. JMS was developed as a tool to make it easier for researchers without a computer science background to make use of HPC. Additionally, JMS can be used to host computational tools and pipelines and generates both web-based interfaces and RESTful APIs for those tools. The web-based interfaces can be used to quickly and easily submit jobs to the underlying cluster. The RESTful web API, on the other hand, allows JMS to provided backend functionality for external tools and web servers that want to run jobs on the cluster. Numerous tools and workflows have already been added to JMS, several of which have been incorporated into external web servers. One such web server is the Human Mutation Analysis (HUMA) web server and database. HUMA, the topic of part 2 of this thesis, is a platform for the analysis of genetic variation in humans. HUMA aggregates data from various existing databases into a single, connected and related database. The advantages of this are realized in the powerful querying abilities that it provides. HUMA includes protein, gene, disease, and variation data and can be searched from the angle of any one of these categories. For example, searching for a protein will return the protein data (e.g. protein sequences, structures, domains and families, and other meta-data). However, the related nature of the database means that genes, diseases, variation, and literature related to the protein will also be returned, giving users a powerful and holistic view of all data associated with the protein. HUMA also provides links to the original sources of the data, allowing users to follow the links to find additional details. HUMA aims to be a platform for the analysis of genetic variation. As such, it also provides tools to visualize and analyse the data (several of which run on the underlying cluster, via JMS). These tools include alignment and 3D structure visualization, homology modeling, variant analysis, and the ability to upload custom variation datasets and map them to proteins, genes and diseases. HUMA also provides collaboration features, allowing users to share and discuss datasets and job results. Finally, part 3 of this thesis focused on the development of a suite of tools, MD-TASK, to analyse genetic variation at the protein structure level via network analysis of molecular dynamics simulations. The use of MD-TASK in combination with the tools developed in the previous parts of this thesis is showcased via the analysis of variation in the renin-angiotensinogen complex, a vital part of the renin-angiotensin system.
- Full Text:
- Date Issued: 2018
- Authors: Brown, David K
- Date: 2018
- Subjects: Bioinformatics , Human genetics -- Variation , High performance computing , Workflow management systems , Molecular dynamics , Next generation sequencing , Human Mutation Analysis (HUMA)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/60708 , vital:27820
- Description: This thesis is divided into three parts, united under the general theme of bioinformatics tool development and variation analysis. Part 1 describes the design and development of the Job Management System (JMS), a workflow management system for high performance computing (HPC). HPC has become an integral part of bioinformatics. Computational methods for molecular dynamics and next generation sequencing (NGS) analysis, which require complex calculations on large datasets, are not yet feasible on desktop computers. As such, powerful computer clusters have been employed to perform these calculations. However, making use of these HPC clusters requires familiarity with command line interfaces. This excludes a large number of researchers from taking advantage of these resources. JMS was developed as a tool to make it easier for researchers without a computer science background to make use of HPC. Additionally, JMS can be used to host computational tools and pipelines and generates both web-based interfaces and RESTful APIs for those tools. The web-based interfaces can be used to quickly and easily submit jobs to the underlying cluster. The RESTful web API, on the other hand, allows JMS to provided backend functionality for external tools and web servers that want to run jobs on the cluster. Numerous tools and workflows have already been added to JMS, several of which have been incorporated into external web servers. One such web server is the Human Mutation Analysis (HUMA) web server and database. HUMA, the topic of part 2 of this thesis, is a platform for the analysis of genetic variation in humans. HUMA aggregates data from various existing databases into a single, connected and related database. The advantages of this are realized in the powerful querying abilities that it provides. HUMA includes protein, gene, disease, and variation data and can be searched from the angle of any one of these categories. For example, searching for a protein will return the protein data (e.g. protein sequences, structures, domains and families, and other meta-data). However, the related nature of the database means that genes, diseases, variation, and literature related to the protein will also be returned, giving users a powerful and holistic view of all data associated with the protein. HUMA also provides links to the original sources of the data, allowing users to follow the links to find additional details. HUMA aims to be a platform for the analysis of genetic variation. As such, it also provides tools to visualize and analyse the data (several of which run on the underlying cluster, via JMS). These tools include alignment and 3D structure visualization, homology modeling, variant analysis, and the ability to upload custom variation datasets and map them to proteins, genes and diseases. HUMA also provides collaboration features, allowing users to share and discuss datasets and job results. Finally, part 3 of this thesis focused on the development of a suite of tools, MD-TASK, to analyse genetic variation at the protein structure level via network analysis of molecular dynamics simulations. The use of MD-TASK in combination with the tools developed in the previous parts of this thesis is showcased via the analysis of variation in the renin-angiotensinogen complex, a vital part of the renin-angiotensin system.
- Full Text:
- Date Issued: 2018
The construction of household livelihood strategies in urban areas: the case of Budiriro, Harare, Zimbabwe
- Authors: Chevo, Tafadzwa
- Date: 2018
- Subjects: Income -- Zimbabwe -- Harare , Cost and standard of living -- Zimbabwe -- Harare , Quality of life -- Zimbabwe -- Harare , Informal sector (Economics) -- Zimbabwe -- Harare , Agricultural wages -- Zimbabwe -- Harare , Households -- Economic aspects -- Zimbabwe -- Harare , Macrosociology , Zimbabwe -- Social conditions -- 1980- , Zimbabwe -- Economic conditions -- 1980- , Livelihoods Framework
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63043 , vital:28357
- Description: The main objective of this thesis is to understand and explain the ongoing construction of livelihood activities by urban households in the low-income high-density area of Budiriro, Harare, Zimbabwe in a context characterised by systemic crisis and a general decline of the national economy. The study utilised a mixed methods research approach, which combined both qualitative and quantitative research, including a survey, life histories and focus group discussions. The thesis discusses a diverse range of livelihood activities of Budiriro households, such as formal employment, informal trading and agricultural activities, and the ways in which households seeks to diversify their livelihood portfolio. It does this by way of also examining the contemporary and historical factors influencing the livelihood activities pursued by these households, along with the shocks and disturbances encountered and experienced by households in trying to construct viable livelihoods. The thesis makes useful contributions to the existing literature on livelihoods studies. Firstly, the thesis disaggregates the households by showing the existence of three wealth categories in Budiriro and the varying livelihood strategies of households in different wealth categories. Secondly, the study highlights the significance of intra-household dynamics in Budiriro for livelihoods as well as of inter-household kinship networks, which transcend the urban space and entail multi-spatial livelihoods. Thirdly, the thesis examines livelihoods over time, such that it goes beyond a strictly synchronic examination, therefore providing a diachronic analysis of diverse and complicated livelihood pathways. Finally, the Livelihoods Framework is located within broader macro-sociological theorising including the work of Pierre Bourdieu. In this respect, important insights arise about livelihood choices and practices in the light of ongoing debates within sociology about human agency.
- Full Text:
- Date Issued: 2018
- Authors: Chevo, Tafadzwa
- Date: 2018
- Subjects: Income -- Zimbabwe -- Harare , Cost and standard of living -- Zimbabwe -- Harare , Quality of life -- Zimbabwe -- Harare , Informal sector (Economics) -- Zimbabwe -- Harare , Agricultural wages -- Zimbabwe -- Harare , Households -- Economic aspects -- Zimbabwe -- Harare , Macrosociology , Zimbabwe -- Social conditions -- 1980- , Zimbabwe -- Economic conditions -- 1980- , Livelihoods Framework
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63043 , vital:28357
- Description: The main objective of this thesis is to understand and explain the ongoing construction of livelihood activities by urban households in the low-income high-density area of Budiriro, Harare, Zimbabwe in a context characterised by systemic crisis and a general decline of the national economy. The study utilised a mixed methods research approach, which combined both qualitative and quantitative research, including a survey, life histories and focus group discussions. The thesis discusses a diverse range of livelihood activities of Budiriro households, such as formal employment, informal trading and agricultural activities, and the ways in which households seeks to diversify their livelihood portfolio. It does this by way of also examining the contemporary and historical factors influencing the livelihood activities pursued by these households, along with the shocks and disturbances encountered and experienced by households in trying to construct viable livelihoods. The thesis makes useful contributions to the existing literature on livelihoods studies. Firstly, the thesis disaggregates the households by showing the existence of three wealth categories in Budiriro and the varying livelihood strategies of households in different wealth categories. Secondly, the study highlights the significance of intra-household dynamics in Budiriro for livelihoods as well as of inter-household kinship networks, which transcend the urban space and entail multi-spatial livelihoods. Thirdly, the thesis examines livelihoods over time, such that it goes beyond a strictly synchronic examination, therefore providing a diachronic analysis of diverse and complicated livelihood pathways. Finally, the Livelihoods Framework is located within broader macro-sociological theorising including the work of Pierre Bourdieu. In this respect, important insights arise about livelihood choices and practices in the light of ongoing debates within sociology about human agency.
- Full Text:
- Date Issued: 2018
Integrating sustainability principles in construction health and safety management practices in Zimbabwe
- Authors: Chigara, Benviolent
- Date: 2018
- Subjects: Sustainable construction -- Zimbabwe , Construction industry -- Safety measures Industrial safety -- Zimbabwe -- Management
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/23459 , vital:30565
- Description: The construction industry is an important industry to national development through the provision of infrastructure and creation of employment, and hence contributing to sustainable development. Nevertheless, the construction industry has a poor health and safety (H&S) and environmental record. Globally, the construction industry is a leading contributor to workplace fatalities, injuries, and disease. In Zimbabwe, the Millennium Towers accident, which resulted in 15 construction workers losing their lives, compromised the integrity of construction H&S management, and highlighted the need to improve H&S management in the sector. This study examined H&S management in Zimbabwe and developed a framework for integrating sustainability into H&S management practices. The study employed a mixed method research design involving the use of interviews and questionnaires to collect primary data from contractors, consultants, government, clients, and workers in Harare and Bulawayo. A total of 101 questionnaires were successfully completed and analysed, representing a response rate of 55.6%. The data from questionnaires were analysed with the help of the SPSS software v.23 to produce descriptive and inferential statistics. The results of the study suggest that H&S practices are inadequate as demonstrated by the extent to which workers are exposed to hazards and the occurrence of injuries, disease, and fatalities. The factors contributing to workers’ exposure to hazards and the occurrence of injuries, disease, and fatalities include, inter alia, inadequate planning for H&S, inadequate hazard identification and risk assessments (HIRAs), inadequate management of hazards, inadequate occupational health (OH) surveillance, appointment of stakeholders who do not systematically manage H&S, inadequate design HIRAs, and unsafe work practices. The problem is amplified by inadequate integration of H&S within procurement systems, which limit the extent to which contractors make financial provision for H&S. Regrettably, workplace fatalities, injuries, and disease diminish the quality of life for the injured workers, increase project cost and delays project completion. Against this background, the study investigated the integration of sustainability principles in construction H&S as a strategy to improve H&S practices in Zimbabwe. The outcome of that investigation was a strategy and interventions, and a framework for improved practices in the form of a Sustainability Framework for Construction H&S (SFCHS). The validation of the SFCHS by construction practitioners confirms the importance of the recommended practices to reducing workplace fatalities, injuries, and disease in Zimbabwe and beyond. The development of a SFCHS, as a strategy to improve H&S practices in Zimbabwe, and the validation thereof, resulted in a significant contribution to the related body of knowledge. Nevertheless, the transition to sustainable H&S practices require contractors, consultants, government, clients and workers to take a proactive role relative to the recommended practices in the SFCHS. Further studies can explore the responsiveness of sustainability factors relative to the occurrence of injuries, disease and fatalities based on selected case studies.
- Full Text:
- Date Issued: 2018
- Authors: Chigara, Benviolent
- Date: 2018
- Subjects: Sustainable construction -- Zimbabwe , Construction industry -- Safety measures Industrial safety -- Zimbabwe -- Management
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/23459 , vital:30565
- Description: The construction industry is an important industry to national development through the provision of infrastructure and creation of employment, and hence contributing to sustainable development. Nevertheless, the construction industry has a poor health and safety (H&S) and environmental record. Globally, the construction industry is a leading contributor to workplace fatalities, injuries, and disease. In Zimbabwe, the Millennium Towers accident, which resulted in 15 construction workers losing their lives, compromised the integrity of construction H&S management, and highlighted the need to improve H&S management in the sector. This study examined H&S management in Zimbabwe and developed a framework for integrating sustainability into H&S management practices. The study employed a mixed method research design involving the use of interviews and questionnaires to collect primary data from contractors, consultants, government, clients, and workers in Harare and Bulawayo. A total of 101 questionnaires were successfully completed and analysed, representing a response rate of 55.6%. The data from questionnaires were analysed with the help of the SPSS software v.23 to produce descriptive and inferential statistics. The results of the study suggest that H&S practices are inadequate as demonstrated by the extent to which workers are exposed to hazards and the occurrence of injuries, disease, and fatalities. The factors contributing to workers’ exposure to hazards and the occurrence of injuries, disease, and fatalities include, inter alia, inadequate planning for H&S, inadequate hazard identification and risk assessments (HIRAs), inadequate management of hazards, inadequate occupational health (OH) surveillance, appointment of stakeholders who do not systematically manage H&S, inadequate design HIRAs, and unsafe work practices. The problem is amplified by inadequate integration of H&S within procurement systems, which limit the extent to which contractors make financial provision for H&S. Regrettably, workplace fatalities, injuries, and disease diminish the quality of life for the injured workers, increase project cost and delays project completion. Against this background, the study investigated the integration of sustainability principles in construction H&S as a strategy to improve H&S practices in Zimbabwe. The outcome of that investigation was a strategy and interventions, and a framework for improved practices in the form of a Sustainability Framework for Construction H&S (SFCHS). The validation of the SFCHS by construction practitioners confirms the importance of the recommended practices to reducing workplace fatalities, injuries, and disease in Zimbabwe and beyond. The development of a SFCHS, as a strategy to improve H&S practices in Zimbabwe, and the validation thereof, resulted in a significant contribution to the related body of knowledge. Nevertheless, the transition to sustainable H&S practices require contractors, consultants, government, clients and workers to take a proactive role relative to the recommended practices in the SFCHS. Further studies can explore the responsiveness of sustainability factors relative to the occurrence of injuries, disease and fatalities based on selected case studies.
- Full Text:
- Date Issued: 2018
Towards intercultural communication congruence in Sino-African organisational contexts
- Authors: Chigwendere, Fungai Beaula
- Date: 2018
- Subjects: Intercultural communication -- Africa , China -- Foreign economic relations -- Africa , Communication in organizations -- Cross-cultural studies , Corporate culture -- Cross-cultural studies , Africa -- Relations -- China , China -- Relations -- Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62395 , vital:28172
- Description: Despite the prevalence of theories and research that could serve as guidelines for addressing intercultural communication challenges such as misunderstanding, ineffectiveness and inappropriateness in communication in Sino-African organisational contexts, the continued existence of these challenges suggests the inadequacy of such theories. Accordingly, in consideration of African and Chinese cultural perspectives, the aim of this study was to develop a hybrid intercultural communication congruence (HICC) framework in order to enhance intercultural communication and achieve intercultural communication congruence in Sino-African organisational contexts. In this study, intercultural communication congruence means effectiveness and appropriateness in intercultural communication. To achieve the study aim, an interpretive pragmatism paradigm was adopted, comprising a two-pronged approach of a synthesis of extant literature and theory as well as an empirical qualitative study, both underpinned by cross-cultural management theory. Based on the synthesis of literature and theory, a generic theoretical intercultural communication congruence framework underpinned by intercultural communication awareness - a state where communicants understand communication orientation and manner of communication in their own and their counterparts’ culture - was developed. A further contribution was a theoretical contextualisation of the generic theoretical intercultural communication congruence framework, resulting in a theoretical framework for intercultural communication awareness in Western, African and Chinese cultures and a theoretical intercultural communication congruence framework specific to Sino-African organisational contexts. Thereafter, with the aim of validating the intercultural communication awareness dimension of the theoretical intercultural communication congruence framework specific to Sino-African organisational contexts, an empirical study was conducted with a sample of seven African and eight Chinese experts using indepth interviews with open-ended and semi-structured questions. Based on qualitative content analysis, six intercultural communication awareness enablers or conditions that enable people to understand communication in different cultures and contexts for the purposes of attaining intercultural communication awareness emerged. These were cultural orientation, manner of communication, orientation to rules and protocol, individual dispositions, intercultural communication influences and intercultural communication variations. An integration of the intercultural communication awareness enablers into the generic theoretical intercultural communication congruence framework results in an updated generic theoretical intercultural communication congruence framework. Then, informed by the updated generic theoretical intercultural communication congruence framework, a further contribution of this study was an empirical verification of the intercultural communication awareness dimension of the theoretical intercultural communication congruence framework specific to Sino-African organisational contexts to result in a final hybrid intercultural communication congruence framework for Sino-African organisational contexts supported by the perspectives of those involved in interaction, rather than predetermined standards of other cultures. Empirical findings suggest that both African and Chinese colleagues maintain a sense of collectivism in the Sino- African organisational context, although this is demonstrated differently. In addition, African colleagues display a blended manner of communication characterised by a mix of Western and African ways while the Chinese manner is contextual and governed by roles and relationships. In African culture, rules and protocol are negotiated, aimed at social maintenance, while strong cultural patriotism ensures strict adherence in Chinese culture. In respect of individual dispositions, African people are seen as open and accommodating while the Chinese disposition could be described as closed and ambiguous. Also evident in the findings is the existence of within-culture differences and influence of noncultural factors on intercultural communication that should be addressed in order to achieve intercultural communication congruence. Finally, the frameworks developed and the methodological processes followed will stimulate academic debate and raise numerous questions for future research. Immediate future research could be geared towards refining the concepts of intercultural communication awareness, intercultural communication congruence and the hybrid intercultural communication congruence framework for Sino-African organisational contexts. At a management practice level, intercultural communication awareness insights provide a reference point for intercultural communication enhancement strategies and interventions in Sino-African organisational contexts.
- Full Text:
- Date Issued: 2018
- Authors: Chigwendere, Fungai Beaula
- Date: 2018
- Subjects: Intercultural communication -- Africa , China -- Foreign economic relations -- Africa , Communication in organizations -- Cross-cultural studies , Corporate culture -- Cross-cultural studies , Africa -- Relations -- China , China -- Relations -- Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62395 , vital:28172
- Description: Despite the prevalence of theories and research that could serve as guidelines for addressing intercultural communication challenges such as misunderstanding, ineffectiveness and inappropriateness in communication in Sino-African organisational contexts, the continued existence of these challenges suggests the inadequacy of such theories. Accordingly, in consideration of African and Chinese cultural perspectives, the aim of this study was to develop a hybrid intercultural communication congruence (HICC) framework in order to enhance intercultural communication and achieve intercultural communication congruence in Sino-African organisational contexts. In this study, intercultural communication congruence means effectiveness and appropriateness in intercultural communication. To achieve the study aim, an interpretive pragmatism paradigm was adopted, comprising a two-pronged approach of a synthesis of extant literature and theory as well as an empirical qualitative study, both underpinned by cross-cultural management theory. Based on the synthesis of literature and theory, a generic theoretical intercultural communication congruence framework underpinned by intercultural communication awareness - a state where communicants understand communication orientation and manner of communication in their own and their counterparts’ culture - was developed. A further contribution was a theoretical contextualisation of the generic theoretical intercultural communication congruence framework, resulting in a theoretical framework for intercultural communication awareness in Western, African and Chinese cultures and a theoretical intercultural communication congruence framework specific to Sino-African organisational contexts. Thereafter, with the aim of validating the intercultural communication awareness dimension of the theoretical intercultural communication congruence framework specific to Sino-African organisational contexts, an empirical study was conducted with a sample of seven African and eight Chinese experts using indepth interviews with open-ended and semi-structured questions. Based on qualitative content analysis, six intercultural communication awareness enablers or conditions that enable people to understand communication in different cultures and contexts for the purposes of attaining intercultural communication awareness emerged. These were cultural orientation, manner of communication, orientation to rules and protocol, individual dispositions, intercultural communication influences and intercultural communication variations. An integration of the intercultural communication awareness enablers into the generic theoretical intercultural communication congruence framework results in an updated generic theoretical intercultural communication congruence framework. Then, informed by the updated generic theoretical intercultural communication congruence framework, a further contribution of this study was an empirical verification of the intercultural communication awareness dimension of the theoretical intercultural communication congruence framework specific to Sino-African organisational contexts to result in a final hybrid intercultural communication congruence framework for Sino-African organisational contexts supported by the perspectives of those involved in interaction, rather than predetermined standards of other cultures. Empirical findings suggest that both African and Chinese colleagues maintain a sense of collectivism in the Sino- African organisational context, although this is demonstrated differently. In addition, African colleagues display a blended manner of communication characterised by a mix of Western and African ways while the Chinese manner is contextual and governed by roles and relationships. In African culture, rules and protocol are negotiated, aimed at social maintenance, while strong cultural patriotism ensures strict adherence in Chinese culture. In respect of individual dispositions, African people are seen as open and accommodating while the Chinese disposition could be described as closed and ambiguous. Also evident in the findings is the existence of within-culture differences and influence of noncultural factors on intercultural communication that should be addressed in order to achieve intercultural communication congruence. Finally, the frameworks developed and the methodological processes followed will stimulate academic debate and raise numerous questions for future research. Immediate future research could be geared towards refining the concepts of intercultural communication awareness, intercultural communication congruence and the hybrid intercultural communication congruence framework for Sino-African organisational contexts. At a management practice level, intercultural communication awareness insights provide a reference point for intercultural communication enhancement strategies and interventions in Sino-African organisational contexts.
- Full Text:
- Date Issued: 2018
Financial market integration, contagion and volatility transmission: a case of the globally developed markets and developing stock markets in Africa
- Authors: Chima, Anyikwa Izunna
- Date: 2018
- Subjects: Money market , Investment analysis Capital market
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/23935 , vital:30646
- Description: The widespread impact of the 2007 global financial crisis and the subsequent Eurozone sovereign debt crisis added new impetus to the on-going international discussions about the sustainability of a financial integration model. Moreover, the crisis revealed the complexity of the international transmission of financial shocks and the financial vulnerabilities of different financial markets. More so, it exposed the major weaknesses in our knowledge of how the forces that drive global financial systems operate. This is compounded by a failure to appreciate the scope of interdependencies that exist across markets and their potential to destabilise the global financial system in times of crises. At the heart of this weakness is the inability to accurately understand the various propagation mechanisms and channels through which a crisis from one market is transmitted to other markets. It is against this background that this study is undertaken, in order to empirically investigate the role of financial market integration, contagion and volatility transmission, using weekly data between the period 3 January 2003 to 26 December 2014. The study covers 27 stock markets, comprising 13 African stock markets, 10 developed stock markets and four emerging stock markets. The study employed two empirical frameworks: the first framework focused on the short-run and long-run relationships between African stock markets and major global stock markets using the Johansen co-integration test, Granger causality test, GIRF and GFEVD. The second framework focused on testing evidence of contagion and volatility transmission using the DCC-GJRGARCH model and AS model. The results show that the majority of African stock markets moved together in the long-run with the major global stock markets during the pre-crisis and Eurozone crisis periods. While the long-run relationship between African stock markets and the major global markets disappeared during the period of the global financial crisis, the relationship re-emerged during the Eurozone crisis period. From the analysis of Granger causality test, the results show some differences exist in terms of the relative strength of the causal linkages across markets and periods. However, it was shown that strong causal linkages emerged during the global financial and Eurozone crisis periods relative to the pre-crisis period. Also, the leading role of the major developed markets, compared to the emerging markets, is demonstrated throughout the analysis of causality tests. Moreover, the sensitivity of African markets to shocks from the global markets was clearly highlighted by analysis of the GIRF and GFEVD, especially during both crisis periods. Furthermore, the results from the AS model confirm significant evidence of mean and volatility spill-over effects from the major global markets to African markets especially during the periods of both crises. In addition, the level of volatility was found to be more persistent and asymmetric during both crisis periods compared to the pre-crisis period. The results confirm the existence of contagion effects through the analysis of the conditional correlation during both crisis periods. More importantly, the analysis of conditional correlation emphasised evidence of heightened co-movement between African markets and the major global markets during the periods of crisis. Consequently, the decoupling phenomenon is rejected in favour of synchronisation of business cycles between African stock markets and the major global markets. The findings of this study have several important implications for the policymakers and investors in Africa and the world at large. The findings of this study not only provide some information about the level of financial integration but also the effect of growing financial linkages between African markets and the global markets, which is important for designing appropriate regulatory frameworks. Also, the knowledge about the dynamic interrelationship in terms of contagion and volatility transmission between African markets and the major global markets can be utilised by investors, and thereby help them to make better investment decisions. Consequently, the findings of this study point to a need for policymakers in general and in Africa in particular, to monitor closely changes in financial development in other markets in order to reduce the vulnerability of domestic markets to external shocks. To mitigate the impact of the external shocks, greater co-operation and co-ordination, with proper supervision of different markets‟ fiscal and monetary policies, should be encouraged. Such policies need to be carefully aligned with the objective of external sustainability. This can be achieved through strategic partnerships and mergers, foreign institutional investments, cross market listing of shares, corporatisation of exchanges and the introduction of private ownership. Above all, effective regulation is needed to realise the benefits of financial market integration.
- Full Text:
- Date Issued: 2018
- Authors: Chima, Anyikwa Izunna
- Date: 2018
- Subjects: Money market , Investment analysis Capital market
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/23935 , vital:30646
- Description: The widespread impact of the 2007 global financial crisis and the subsequent Eurozone sovereign debt crisis added new impetus to the on-going international discussions about the sustainability of a financial integration model. Moreover, the crisis revealed the complexity of the international transmission of financial shocks and the financial vulnerabilities of different financial markets. More so, it exposed the major weaknesses in our knowledge of how the forces that drive global financial systems operate. This is compounded by a failure to appreciate the scope of interdependencies that exist across markets and their potential to destabilise the global financial system in times of crises. At the heart of this weakness is the inability to accurately understand the various propagation mechanisms and channels through which a crisis from one market is transmitted to other markets. It is against this background that this study is undertaken, in order to empirically investigate the role of financial market integration, contagion and volatility transmission, using weekly data between the period 3 January 2003 to 26 December 2014. The study covers 27 stock markets, comprising 13 African stock markets, 10 developed stock markets and four emerging stock markets. The study employed two empirical frameworks: the first framework focused on the short-run and long-run relationships between African stock markets and major global stock markets using the Johansen co-integration test, Granger causality test, GIRF and GFEVD. The second framework focused on testing evidence of contagion and volatility transmission using the DCC-GJRGARCH model and AS model. The results show that the majority of African stock markets moved together in the long-run with the major global stock markets during the pre-crisis and Eurozone crisis periods. While the long-run relationship between African stock markets and the major global markets disappeared during the period of the global financial crisis, the relationship re-emerged during the Eurozone crisis period. From the analysis of Granger causality test, the results show some differences exist in terms of the relative strength of the causal linkages across markets and periods. However, it was shown that strong causal linkages emerged during the global financial and Eurozone crisis periods relative to the pre-crisis period. Also, the leading role of the major developed markets, compared to the emerging markets, is demonstrated throughout the analysis of causality tests. Moreover, the sensitivity of African markets to shocks from the global markets was clearly highlighted by analysis of the GIRF and GFEVD, especially during both crisis periods. Furthermore, the results from the AS model confirm significant evidence of mean and volatility spill-over effects from the major global markets to African markets especially during the periods of both crises. In addition, the level of volatility was found to be more persistent and asymmetric during both crisis periods compared to the pre-crisis period. The results confirm the existence of contagion effects through the analysis of the conditional correlation during both crisis periods. More importantly, the analysis of conditional correlation emphasised evidence of heightened co-movement between African markets and the major global markets during the periods of crisis. Consequently, the decoupling phenomenon is rejected in favour of synchronisation of business cycles between African stock markets and the major global markets. The findings of this study have several important implications for the policymakers and investors in Africa and the world at large. The findings of this study not only provide some information about the level of financial integration but also the effect of growing financial linkages between African markets and the global markets, which is important for designing appropriate regulatory frameworks. Also, the knowledge about the dynamic interrelationship in terms of contagion and volatility transmission between African markets and the major global markets can be utilised by investors, and thereby help them to make better investment decisions. Consequently, the findings of this study point to a need for policymakers in general and in Africa in particular, to monitor closely changes in financial development in other markets in order to reduce the vulnerability of domestic markets to external shocks. To mitigate the impact of the external shocks, greater co-operation and co-ordination, with proper supervision of different markets‟ fiscal and monetary policies, should be encouraged. Such policies need to be carefully aligned with the objective of external sustainability. This can be achieved through strategic partnerships and mergers, foreign institutional investments, cross market listing of shares, corporatisation of exchanges and the introduction of private ownership. Above all, effective regulation is needed to realise the benefits of financial market integration.
- Full Text:
- Date Issued: 2018
Provision of psychosocial support to vulnerable learners by peer led groups in secondary schools in Amathole West Education District, Eastern Cape
- Authors: Chinyama, Nyarayi
- Date: 2018
- Subjects: Psychosocial support -- Education, Secondary -- South Africa -- Eastern Cape Education, Secondary -- Study and teaching -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9341 , vital:34333
- Description: This study sought provision of psychosocial support to vulnerable learners by school based peer-led groups in secondary schools. Using the Ecological Systems Theory and the Psychosocial Support Interactive Process Model, the study specifically sought how vulnerable learners were identified, how participants understood vulnerability and psychosocial support, how peer led groups were selected and the strategies used to provide psychosocial support by peer led groups. The study also sought the support and monitoring strategies used by different stakeholders including the department of education inorder to assit the peer led groups in their endevour to provide psychosocial support to vulnerable learners. The study employed qualitative research methods using interviews, focus groups and document analysis. Purposive sampling was used to select a District Education Officer, four principals, four group members and all of whom took part in interviews, then twenty four peer group members who participated in focus group discussions. The study found out that various methods were used to identify vulnerable learners, but these were inadequate due to limited professional support to the peer-led groups. The strategies employed to assist vulnerable learners included the buddy system, scripture unions, boys and girls education movements and home visits. However there were also challenges of shortage of human and material resources such as counselling rooms, transport. Also none cooperation of parents still hampers psychosocial support by peer led groups. Therefore, the study concludes that there are still a lot of vulnerable learners who are not reached by peer-led groups because of the above mentioned challenges. In this light the study proposed a framework for providing psychosocial support. The study therefore, recommends that the psychosocial programme be part of the large school curriculum which has a specific budget to sustain all the identification, support and monitoring needs of the vulnerable learners and to ensure the psychosocial support strategies are successful.
- Full Text:
- Date Issued: 2018
- Authors: Chinyama, Nyarayi
- Date: 2018
- Subjects: Psychosocial support -- Education, Secondary -- South Africa -- Eastern Cape Education, Secondary -- Study and teaching -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9341 , vital:34333
- Description: This study sought provision of psychosocial support to vulnerable learners by school based peer-led groups in secondary schools. Using the Ecological Systems Theory and the Psychosocial Support Interactive Process Model, the study specifically sought how vulnerable learners were identified, how participants understood vulnerability and psychosocial support, how peer led groups were selected and the strategies used to provide psychosocial support by peer led groups. The study also sought the support and monitoring strategies used by different stakeholders including the department of education inorder to assit the peer led groups in their endevour to provide psychosocial support to vulnerable learners. The study employed qualitative research methods using interviews, focus groups and document analysis. Purposive sampling was used to select a District Education Officer, four principals, four group members and all of whom took part in interviews, then twenty four peer group members who participated in focus group discussions. The study found out that various methods were used to identify vulnerable learners, but these were inadequate due to limited professional support to the peer-led groups. The strategies employed to assist vulnerable learners included the buddy system, scripture unions, boys and girls education movements and home visits. However there were also challenges of shortage of human and material resources such as counselling rooms, transport. Also none cooperation of parents still hampers psychosocial support by peer led groups. Therefore, the study concludes that there are still a lot of vulnerable learners who are not reached by peer-led groups because of the above mentioned challenges. In this light the study proposed a framework for providing psychosocial support. The study therefore, recommends that the psychosocial programme be part of the large school curriculum which has a specific budget to sustain all the identification, support and monitoring needs of the vulnerable learners and to ensure the psychosocial support strategies are successful.
- Full Text:
- Date Issued: 2018