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Benchmarking a Decision Support System for Aquatic Toxicity Testing

- Griffin, Neil J, Odume, Oghenekaro N, Mensah, Paul K, Palmer, Carolyn G

Authors: Griffin, Neil J , Odume, Oghenekaro N , Mensah, Paul K , Palmer, Carolyn G
Date: 2019
Subjects: To be catalogued
Language: English
Type: text , report
Identifier: http://hdl.handle.net/10962/437798 , vital:73413 , ISBN 978-0-6392-0108-5 , https://wrcwebsite.azurewebsites.net/wp-content/uploads/mdocs/2445_final1.pdf
Description: This book was written for the catchment management forum (CMF) in the Upper Komati Forum (UKF), and they share their experience in order to help other CMFs understand the damage coal mining does to our water resources. This booklet should be used with How to think and act in ways that make Adaptive IWRM practi-cally possible and How to establish and run a Catchment Management Forum.
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Between drones and al-Shabaab: United States extra-judicial killings in Somalia, sovereignty and the future of liberal intervention

- Koloko, Mojalefa

Authors: Koloko, Mojalefa
Date: 2019
Subjects: Somalia -- Politics and government -- 1991- , Somalia -- History -- 1991- , Somalia -- Foreign relations -- United States , Somalia -- Foreign relations -- 1991- , Military assistance, American -- Somalia , Extrajudicial exeutions -- Somalia , Shabaab (Organization)
Language: English
Type: text , Thesis , Masters , MA
Identifier: http://hdl.handle.net/10962/67657 , vital:29125
Description: This study examines the nature of the United States intervention in Somalia, specifically the use of drone strikes that first targeted the militant Sunni Islamist transnational group, al-Qaeda, which claimed responsibility for the 11 September 2001 attacks on the United States, and now target the Somali organisation, Harakat al-Shabaab al-Mujahidin, commonly known as al-Shabaab. The use of drone strikes in the US led war on terror has raised concerns about sovereignty as the extra-judicial killings are conducted without the consent of the concerned states. Furthermore, drone strikes also raise questions about the processes of liberal intervention as the US conducts them without the approval of the United Nations Security Council. It is argued in this study that what is understood to be the “golden era” of liberal interventionism is a legacy of the post-Cold War unipolar dominance of the United States in global governance and security. It is argued that US unipolarity was accompanied by a shifting perception regarding the security position of weak states, whose weakness becomes understood as a source of global insecurity. This perception that so called “weak” and “fragile” states are sources of threats is a departure in International Relations theory, because the discipline is historically preoccupied with studying the actions of powerful states and their consequence for the global order. It is argued that the discourse on the war on terror, and its focus on “failed states” as breeding grounds for alleged terrorists, represents the height of the repositioning of less powerful states from a peripheral status in IR analysis and practice, to their current position that are now being represented as core sources of threat to international peace and security. Through life history interviews with Somali nationals in Grahamstown, Port Elizabeth and Uitenhage, South Africa, the study examines the consequences of US actions from the eyes of Somali people. The findings of this study show that despite all the controversy surrounding the legitimacy of the war on terror, the manner in which it is conducted, as well as the tactics that it employs, the majority of Somali participants showed an overwhelming support for the US intervention. Participants expressed support for the US extra-judicial killings because they are understood to undermine al-Shabaab strength which is a major source of insecurity. The study also shows that the lack of necessary collaboration between the US intelligence and the Somali ground forces has resulted in high numbers of civilian deaths, which participants fear can be used by al-Shabaab to recruit and radicalise more Somalis. The study also shows that most Somalis resent the presence of the African Union Mission in Somalia because Kenya and Ethiopia are seen as 10 pursuing national interests that are not invested in Somali peace and stability. The study concludes that US extra-judicial killings have failed to constrain the actions of al-Shabaab. Somalis expressed that the leadership of current president, Mohamed Abdullahi Mohamed Farmaajo, holds the unique possibilities of creating national unity that rises above clan divisions and the radical Jihadist ideology of al-Shabaab.
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Biochemical characterization of the β-mannanase activity of Bacillus paralicheniformis SVD1

- Clarke, Matthew David

Authors: Clarke, Matthew David
Date: 2019
Subjects: Mycobacterium avium paratuberculosis , Enzymes -- Biotechnology , Lignocellulose -- Biotechnology
Language: English
Type: text , Thesis , Masters , MSc
Identifier: http://hdl.handle.net/10962/67570 , vital:29112
Description: Products produced via the enzymatic hydrolysis of lignocellulosic biomass, the most abundant renewable terrestrial source of carbon, can potentially replace a lot of the fuels and chemicals currently produced using non-renewable hydrocarbons. Mannan is a polysaccharide component of lignocellulose that is abundant in softwoods and legume seeds. Enzymatic hydrolysis of mannan by β-mannanases has various industrial applications, including use in biofuel and prebiotic mannooligosaccharide (MOS) production for the improvement of human and animal health. The industrial use of β-mannanases depends on their biochemical characteristics, such as their activity, stability and substrate specificity. Knowledge of their synergistic interactions with other enzymes is also useful for effective hydrolysis. Bacillus paralicheniformis SVD1 was used as a source for β-mannanases. The two mannanases of B. paralicheniformis SVD1 have not been biochemically characterized apart from minor characterization of crude β-mannanase activity. The protein sequences of the two β-mannanases, of glycosyl hydrolase family 5 and 26, have a 95% - 96% identity to the β-mannanases of B. licheniformis DSM13T (=ATCC14580T). These small protein sequence differences could lead to quite different biochemical characteristics. These mannanases were characterized as these enzymes may have industrially useful characteristics. To induce mannanase production, B. paralicheniformis SVD1 was cultured in broth containing the mannan substrate locust bean gum. Various growth curve parameters were measured over 72 h. Mannanase activity was the highest after 48 h of growth - this was the time at which mannanase activity was concentrated, using 3 kDa centrifugal filtration devices, for biochemical characterization of the crude activity. Zymography revealed that the crude concentrated mannanase fraction consisted of at least two mannanases with relative molecular weights (MWs) of 29.6 kDa and 33 kDa. This was smaller than expected – based on their theoretical molecular masses. Protease activity, which was detected in the broth, was probably the reason. There were two pH optima, pH 5.0 and pH 7.0, which also indicated the presence of two mannanases. The concentrated mannanase displayed characteristics that were expected of a B. paralicheniformis β-mannanase. The temperature optimum was 50°C and the activity loss was less than 7% at 50°C after 24 h. Substrate specificity assays revealed that there was predominantly mannanase activity present. Thin layer chromatography (TLC) analysis of mannan and MOS hydrolysis showed that mainly M2 and M3 MOS were produced; only MOS with a degree of polymerization of 4 or higher were hydrolyzed. Hydrolysis was minimal on mannoligosaccharides with galactose substituents. Activity and MOS production was the highest on soluble, low branched mannan substrates. The highest activity observed was on konjac glucomannan. Purification of the mannanase activity was then attempted using various methods. Ammonium sulfate precipitation, acetone precipitation, as well as centrifugal filtration device concentration was assessed for concentration of the mannanase activity.Concentration was not very successful due to low activity yields (≤ 20%). Anion exchange chromatography (AEC) and size exclusion chromatography (SEC) was used for purification. AEC gave good activity yield and fold purification, but SDS-PAGE analysis revealed the presence of many different proteins so further purification was necessary. SDS-PAGE analysis showed that there were only a few protein contaminants in the SEC fraction. However, the yield was too low to allow for biochemical characterization. The optimized purification procedure, which partially purified the mannanase activity, used 85% ammonium sulfate precipitation, followed by AEC. The fold purification was high (88.9) and the specific activity was 29.5 U.mg-1. A zymogram of the partially purified mannanase showed a mannanase active band with a MW of 40 - 41 kDa. A serine protease inhibitor, phenylmethylsulfonyl fluoride (PMSF), was added during the purification steps. This indicated that the mannanase/s in the crude concentrate, without PMSF added, was hydrolyzed by serine protease activity. Native PAGE zymograms suggested that at least two different isoforms of mannanases were present. Additional purification would be required to determine the true characteristics of the mannanase/s. The biochemical characteristics of the crude and partially purified mannanases were similar. The pH optima of the partially purified mannanases were different; the pH optima were 6.0 and 9.0. The substrate specificities were similar, except that the partially purified mannanases displayed no cellulase and β-D-galactosidase activity, but showed a small amount of α-L-arabinase activity. The partially purified mannanase and a Cyamopsis tetragonolobus GH27 α-galactosidase synergistically hydrolyzed locust bean gum. The M50G50 combination displayed the highest extent of hydrolysis; after 24 h there was a 1.39 fold increase in reducing sugar release and the degree of synergy (DS) was 4.64. TLC analysis indicated that synergy increased the release of small MOS. These MOS could be useful as prebiotics. The synergy between the partially purified mannanase and the commercial cellulase mixture Cellic® CTec2 (Novozymes) on spent coffee grounds (SCG) was also determined. SCG is an abundant industrial waste product that has high mannan content. The SCG was pretreated using NaOH, and the monosaccharide, soluble phenolics and insoluble contents were determined. Glucose and mannose were the dominant monosaccharides in the SCG; the pretreated SCG contained 20.4% (w/w) glucose and 18.5% (w/w) mannose, respectively. The NaOH pretreatment improved mannanase hydrolysis of SCG. It resulted in the opening up and swelling of the SCG particles and removed some of the insoluble solids. The partially purified B. paralicheniformis SVD1 mannanase displayed no detectable activity on SCG, but showed synergy with CTec2, in terms of DS, on untreated and NaOH pretreated SCG. This is the first report of mannanasecellulase synergy on SCG; other studies found that increased hydrolysis was due to additive effects. The results obtained in this study are only an initial assessment of the biochemical properties of B. paralicheniformis SVD1 mannanase activity and its synergy with other enzymes. These results can be used to inform future studies.
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Biological properties and interactions of Kalaharituber pfeilii

- Krele, Viwe

Authors: Krele, Viwe
Date: 2019
Subjects: Kalaharituber pfeilii , Pezizales -- South Africa , Desert plants -- South Africa , Truffle culture -- South Africa , Plant biochemical genetics , Enzymes -- Analysis
Language: English
Type: text , Thesis , Masters , MSc
Identifier: http://hdl.handle.net/10962/72257 , vital:30022
Description: Dessert truffles are seasonal macro fungi and have been identified in several parts of the world including South Africa. The first part of the present study dealt with the assessment of the biologically active compounds of the Kalahari truffles found in the Northern Cape of South Africa. Truffles extracts (methanol, ethanol, aqueous) were investigated for their antimicrobial properties towards Gram-positive and Gram-negative bacteria. The results demonstrated that the truffle extracts tested had no inhibitory effects against the bacterial isolates. The truffle mycelial growth was also noted to be ineffective against the selected bacteria. The bacteria tested in the present study showed some antagonistic effects against the fungus. Cultures of K. pfeilii were also screened for enzyme production including amylase, protease, cellulose, and laccase. Evaluation of the potential of K. pfeilii mycelia to produce these industrially and economically important enzymes demonstrated both amylase and protease activity. However, for laccase and cellulose, no activity was detected. The second part of the present study aimed at optimizing biomass production by K. pfeilii in liquid culture media. FF Microplate containing 95 discreet carbon sources were employed to test for substrate utilization. Blanked readings above 0.1 were regarded as positive for utilization, and 4 substrates were selected as potential substrates and were included in liquid media. Media was evaluated for mycelial biomass production. Of the carbon sources tested sucrose proved to be the most suitable for supporting mycelial growth. The third part of the current study included investigating the diversity of microbial communities colonizing the rhizosheath of Stipagrostis ciliata var. capensis (the host plant of K. pfeilii) and these were identified by means of next-generation sequencing using Illumina Miseq. Bioinformatics tools were utilized in analyzing the data. Actinobacteria were found to be the most dominant bacterial phylum, followed by unclassified bacteria, Proteobacteria, and Acidobacteria. The top 25 sequences were selected and clustered into bacterial OTUs (at 97% threshold) which were assigned into 1 phylum (Actinobacteria), 1 family (Geodermatophilaceae) and 23 genera. This phylum is well known for its secondary metabolites. Streptomyces sp. was the most frequently encountered genus. The results from this study necessitate further investigations with regards to the function and evolution of fungal-bacterial associations. Wheather these bacteria have a contribution towards the truffle development, it is still not confirmed.
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Bloodmeal analysis in Culicoides midges collected near horses, donkeys and zebras in the Eastern Cape, South Africa:

- Riddin, Megan A, Venter, G J, Labuschagne, K, Villet, Martin H

Authors: Riddin, Megan A , Venter, G J , Labuschagne, K , Villet, Martin H
Date: 2019
Language: English
Type: text , article
Identifier: http://hdl.handle.net/10962/140692 , vital:37910 , DOI: 10.1111/mve.12381
Description: An upsurge in African horse sickness (AHS) in the Eastern Cape, South Africa, from 2006 led to an epidemiological reassessment of the disease there. Light trapping surveys carried out near horses, donkeys and zebras in 2014–2016 collected 39 species of Culicoides midge (Diptera: Ceratopogonidae) that are potential vectors of AHS. To establish if these midges fed on equids, DNA sequences were obtained from the gut contents of 52 female midges (35 freshly blood-fed, 13 gravid and four parous), representing 11 species collected across 11 sites. Culicoides leucostictus fed on all three equids. Culicoides bolitinos, Culicoides imicola and Culicoides magnus fed on both horses and donkeys. Culicoides onderstepoortensis fed on donkeys, and Culicoides similis and Culicoides pycnostictus fed on zebras. Bloodmeals from cows, pigs, warthogs, impalas and a domestic dog were also identified in various species, but none of the midges tested had fed on birds. These results contribute to knowledge of the vectorial capacity of several species of Culicoides with regard to AHS in the Eastern Cape and point to potential reservoir hosts, of which donkeys, zebras and domestic dogs have previously been found to harbour AHS. Blood-fed midges were also obtained throughout winter, indicating the potential for endemic AHS in the province.
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BODIPY and porphyrin dyes for direct glucose sensing and optical limiting applications

- Ndebele, Nobuhle

Authors: Ndebele, Nobuhle
Date: 2019
Subjects: Boron compounds , Boric acid , Porphyrins , Dyes and dying -- Chemistry
Language: English
Type: text , Thesis , Masters , MSc
Identifier: http://hdl.handle.net/10962/97221 , vital:31412
Description: A series of BODIPY dyes functionalised with boronic acid in the 3,5-positions were successfully synthesised and characterised by using various analytical techniques. The dyes were prepared through a slight modification of the conventional acid catalysed condensation method. Phenylboronic acid moieties were added as styryl groups at the 3,5-positions of the 1,3,5,7-tetrametylBODIPY cores using a modified Knoevengal condensation method. The addition of the styryls resulted in the main absorption band of the dyes red-shifting to the 630−650 nm region. The photophysical and electrochemical properties of these dyes were studied to determine whether the dyes are suitable for use in the fluorescent, colourimetric and electrochemical detection of glucose. Boronic acid moieties were added as bioreceptor recognition elements because they have an affinity for carbohydrates and therefore would be able to bind and “detect” glucose. The series of BODIPY dyes did not show a “turn-on” fluorescence effect upon addition with glucose at the physiological pH. This was attributed on the basis of molecular modelling to the absence of an MO localised on the boronic-acid-substituted styryl moieties that lie close in energy to the HOMO and LUMO that facilitates the formation of an intramolecular charge transfer state. However, colourimetric changes that are visible to the naked eye are observed at basic pH when glucose was added to the dye solutions. The dyes exhibited favourable electrochemical behaviour and were able to detect glucose directly in this context when glassy carbon electrodes are modified through the drop dry method. A series of Sn(IV) porphyrins with thienyl and phenyl groups at the meso-positions were successfully synthesised and characterised. Pyridine and tetrabutyl axial ligands were added to the porphyrins to limit aggregation. The optical limiting properties of these porphyrins and three styrylated BODIPY dyes were studied in benzene and dichloromethane. Dyes were also embedded in polystyrene and studied as thin films to further gauge their suitability for use in optical limiting applications. Second-order hyperpolarizability, third-order susceptibly, non-linear absorption with reversible saturable absorption and the optical limiting threshold, were the parameters studied. Three of the four porphyrins and the three styrylated BODIPY dyes showed favourable optical limiting behaviour, which was further enhanced when the dyes are embedded in polymer thin films.
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Bolvedere: a scalable network flow threat analysis system

- Herbert, Alan

Authors: Herbert, Alan
Date: 2019
Subjects: Bolvedere (Computer network analysis system) , Computer networks -- Scalability , Computer networks -- Measurement , Computer networks -- Security measures , Telecommunication -- Traffic -- Measurement
Language: English
Type: text , Thesis , Doctoral , PhD
Identifier: http://hdl.handle.net/10962/71557 , vital:29873
Description: Since the advent of the Internet, and its public availability in the late 90’s, there have been significant advancements to network technologies and thus a significant increase of the bandwidth available to network users, both human and automated. Although this growth is of great value to network users, it has led to an increase in malicious network-based activities and it is theorized that, as more services become available on the Internet, the volume of such activities will continue to grow. Because of this, there is a need to monitor, comprehend, discern, understand and (where needed) respond to events on networks worldwide. Although this line of thought is simple in its reasoning, undertaking such a task is no small feat. Full packet analysis is a method of network surveillance that seeks out specific characteristics within network traffic that may tell of malicious activity or anomalies in regular network usage. It is carried out within firewalls and implemented through packet classification. In the context of the networks that make up the Internet, this form of packet analysis has become infeasible, as the volume of traffic introduced onto these networks every day is so large that there are simply not enough processing resources to perform such a task on every packet in real time. One could combat this problem by performing post-incident forensics; archiving packets and processing them later. However, as one cannot process all incoming packets, the archive will eventually run out of space. Full packet analysis is also hindered by the fact that some existing, commonly-used solutions are designed around a single host and single thread of execution, an outdated approach that is far slower than necessary on current computing technology. This research explores the conceptual design and implementation of a scalable network traffic analysis system named Bolvedere. Analysis performed by Bolvedere simply asks whether the existence of a connection, coupled with its associated metadata, is enough to conclude something meaningful about that connection. This idea draws away from the traditional processing of every single byte in every single packet monitored on a network link (Deep Packet Inspection) through the concept of working with connection flows. Bolvedere performs its work by leveraging the NetFlow version 9 and IPFIX protocols, but is not limited to these. It is implemented using a modular approach that allows for either complete execution of the system on a single host or the horizontal scaling out of subsystems on multiple hosts. The use of multiple hosts is achieved through the implementation of Zero Message Queue (ZMQ). This allows for Bolvedre to horizontally scale out, which results in an increase in processing resources and thus an increase in analysis throughput. This is due to ease of interprocess communications provided by ZMQ. Many underlying mechanisms in Bolvedere have been automated. This is intended to make the system more userfriendly, as the user need only tell Bolvedere what information they wish to analyse, and the system will then rebuild itself in order to achieve this required task. Bolvedere has also been hardware-accelerated through the use of Field-Programmable Gate Array (FPGA) technologies, which more than doubled the total throughput of the system.
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Book Review: From the Outside In

- Magadla, Siphokazi

Authors: Magadla, Siphokazi
Date: 2019
Subjects: To be catalogued
Language: English
Type: text , article
Identifier: http://hdl.handle.net/10962/298628 , vital:57722 , xlink:href="https://doi.org/10.1080/10220461.2019.1610042"
Description: From the Outside In: Domestic Actors and South Africa’s Foreign Policy presents an exciting collection of chapters that explore various and often-ignored domestic actors and their relationships within the state, including government departments and local government; and non-governmental organisations such as labour unions, big business and social movements. The editors evoke the imagery of the ‘chess game of foreign policy’, used by scholars such as Joseph Nye,1 to argue that power in international relations is distributed like a ‘three-dimensional chessboard’ consisting of military power, economic power and transnational relations.
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Boundary-crossing learning in agricultural learning systems: formative interventions for water and seed provision in southern Africa

- Pesanayi, Victor Tichaona

Authors: Pesanayi, Victor Tichaona
Date: 2019
Subjects: Agriultural extension work -- Africa, Southern , Agriultural colleges -- Africa, Southern , Farmers -- Education -- Africa, Southern , Agriculture and state -- Africa, Southern , Sustainable agriculture -- Africa, Southern
Language: English
Type: text , Thesis , Doctoral , PhD
Identifier: http://hdl.handle.net/10962/94067 , vital:30997
Description: This research was conducted in the Amathole rural district of the Eastern Cape Province of South Africa, and in Zvishavane and Zhombe rural districts of the Midlands Province of Zimbabwe over a period of four years. In the first two years of this period I was involved in co-engaged boundary-crossing expansive learning processes with research participants from agricultural education (agricultural college lecturers, principals and university lecturers), extension services (extension officers, advisors and workers), small-scale farmers and a local economic development (LED) agency as agricultural learning activity systems. The latter was applicable only to the South African nested case while the rest applied to both country nested cases. The study focusses on the boundary-crossing learning of sustainable agricultural water relevant for small-scale farming contexts under rain-fed and climate constrained conditions with specific attention to rainwater harvesting and conservation and climate-adaptive seed. The study employed cultural-historical activity theory (CHAT) and developmental work research methodology developed by Yrjö Engeström and his colleagues at the Centre for Researching Activity Development and Learning (CRADLE) at the University of Helsinki in Finland. The study was guided by three objectives. The first objective was to find out how the different groups represented across the activity systems listed above learn together to mediate and communicate sustainable agricultural water and seed saving. To address this objective I conducted focus groups and interviews with key informants, made observations and analysed documents. The second objective was to explore and document the socio-ecological histories of rainwater harvesting and conservation, locally-adaptive seed systems and associated value chains, and socio-cultural histories of agricultural learning systems in the context of small-scale farming using historical and ethnographic research techniques. The third objective was to understand how learning, curriculum innovation and mediation tools for agricultural extension education and farmer training that can expand learning of rainwater harvesting and conservation sustainable practices for improved local agricultural water and climate-adaptive non-formal seed systems in agricultural education and small-scale farmer activity systems could be co-generated. This third objective constituted the boundary-crossing expansive learning that emerged from change laboratory workshops carefully designed to explore the common water for food object across the different but related activity systems. The study reveals historically-persisting tensions and contradictions in the work of agricultural college lecturers, small-scale farmers and extension workers that limit their ability to work together relationally leaving them operating in isolated ‘silos’. The industrially-driven agricultural college curriculum promoting conventional irrigated agriculture conflicted with the college’s objective of producing extension workers who will work with resource-poor small-scale farmers in rain-fed farming systems. This conflict was aggravated by the work of extension workers who had little to no knowledge regarding how to support small-scale farmers facing persistent drought and consequent crop failure due to poor and erratic rainfall. At the same time extension services promoting genetically modified (GMO) seed in South Africa were in conflict with some small-scale farmers’ demands for seed that was adapted to their changing climate and their ability to operate independently with access to and ownership of land. This study shows that the work of agricultural colleges and extension services often defeats its intended structural objectives due to historically-constituted power relations around knowledge. This study has demonstrated the effectiveness of co-generative formative interventions in boundary-crossing scenarios in learning network contexts for expansion of activity in farming communities, agricultural colleges and extension services, with emphasis of transformed activity towards engaging a collective object of rainwater harvesting and conservation for more sustainable agriculture and poverty alleviation. The study shows that diverse combinations of change practice courses, change laboratories, demonstration sites and media engagements as mediation processes in the context of learning networks strengthened the possibility for boundary-crossing expansive learning across activity systems of agricultural college lecturers, smallholder farmers, extension workers and local economic development agency facilitators. Three of the five mediation processes emerged out of the formative intervention processes in both the South African and Zimbabwean case studies while two were not realised in the Zimbabwean case study, namely the change practice course and media engagements, due to different formative intervention conditions, inadequate time and resources. Boundary-crossing was enabled by a variety of actions including understanding and identifying with the context of the other (i.e. developing empathy) as a result of change laboratory workshops that also ensured confrontation with relational contradictions. The study concludes that it is possible for historically-constituted contradictions around water for food to be resolved when participants from different agricultural learning systems co-engage as equals in boundary-crossing change laboratory fora mediated by appropriate tools and processes. The study contributes to innovation in agricultural learning systems in southern Africa, in particular to means of engaging across boundaries of previously largely disconnected activity systems in ways that benefit smallholder farmers who have previously been marginalised from mainstream agricultural learning systems.
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Bushmeat use is widespread but under-researched in rural communities of South Africa

- Martins, Vusumzi, Shackleton, Charlie M

Authors: Martins, Vusumzi , Shackleton, Charlie M
Date: 2019
Subjects: To be catalogued
Language: English
Type: text , article
Identifier: http://hdl.handle.net/10962/179745 , vital:43167 , xlink:href="https://doi.org/10.1016/j.gecco.2019.e00583"
Description: Bushmeat hunting and consumption is common throughout sub-Saharan Africa. Yet, a recent review indicated that the prevalence and nature of bushmeat hunting was little researched or understood in southern African savannas. Here we present information from a number of rural livelihoods studies in South Africa that indicate that bushmeat consumption is common, with typically between 30 and 60% of rural households in the communal tenure regions stating that they consume it. Yet there are only five studies in the country explicitly investigating bushmeat hunting practices, motivations, offtake and target species. A review of the five studies indicates that bushmeat hunting is largely a male activity and that motivations and practices vary between sites. Hunting with dogs is the most common method, targeting multiple small and medium-sized species. With such widespread consumption, it is possible that bushmeat hunting may have significant effects on the population status of some target species and consequently requires urgent and in-depth research of both practices and effects
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Busy as a Bee: Breeding Industrious Bees in Malawi

- Kasumba, Arnold M, Pullanikkatil, Deepa

Authors: Kasumba, Arnold M , Pullanikkatil, Deepa
Date: 2019
Subjects: To be catalogued
Language: English
Type: text , book chapter
Identifier: http://hdl.handle.net/10962/433537 , vital:72982 , ISBN 978-3-319-75580-9 , https://link.springer.com/chapter/10.1007/978-3-319-75580-9_14
Description: A trial and error venture at beekeeping by Arnold Kasumbu in central Malawi, is now a successful integrated farming enterprise incorporating 160 beehives and producing honey for customers in Malawi and Kenya. It is also a site for educational visits for university students learning about agriculture. Arnold’s parents were tobacco farmers living in the Mitundu area of Lilongwe District in central Malawi. He recalls that his childhood was steeped in poverty as he observed his father put in a lot of effort in his farm with diminutive returns. Being the eldest of three children in the household, Arnold was forced to end his schooling in Standard 8, as his family could not afford the school fees. Resolving that he would not do tobacco farming, he began exploring other means of income generation that would entail fewer struggles. He wanted to become wealthy and take care of his family. Through his integrated farm and beekeeping, he has done exactly that! This is Arnold’s life story.
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Careerism and capitalism as women’s emancipation: a critical analysis of Rand Merchant Bank’s ‘Athena Programme', South Africa

- Mosesi, Poloko Prudence

Authors: Mosesi, Poloko Prudence
Date: 2019
Subjects: Rand Merchant Bank (South Africa) , Women in economic development -- South Africa , Women in finance -- South Africa , Neoliberalism -- South Africa
Language: English
Type: text , Thesis , Masters , MSocSc
Identifier: http://hdl.handle.net/10962/140344 , vital:37881
Description: Against the backdrop of debates on feminism and neo– liberalism, this thesis presents a critical analysis of Rand Merchant Bank’s (RMB) Athena programme in South Africa – an award– winning programme run by women, which aims to develop women as senior managers – and of the type of feminism it represents, using a Marxist feminist theoretical framework. Rand Merchant Bank’s is one of the largest investment banks in Africa, part of the giant First Rand Group (FRG) alongside First National Bank (FNB) group. The thesis, based on a detailed case study using qualitative methods, argues that Athena is a very much product of its time: it advances the argument that if more women were in position of power, women in general would be free, and it views the problem of women’s oppression with an individualist lens, which focuses on attitudes and confidence. Athena, like many initiatives of the neo– liberal era, such as Sandberg’s Lean– In philosophy and the Nike Foundation’s ‘Girl Effect’ promote individual understanding and emancipation of women, which sees emancipation in terms of creating a neo– liberal subject that operates more effectively within a capitalist framework, sees capitalism as the solution – rather than the cause – of women’s unequal circumstance and ignores structural issues like class. In effect, Athena argues that the free market and big corporations are neutral tools that can answer the question of women equality, if only women had the correct attitudes, and so long as corporate hierarchies and profits are accepted as fair, and women are seen as an untapped resource that can be used in a ‘smart economics.’ What all these initiatives have in common is shifting the burden and responsibility to women without proper interrogation of the systems that perpetuate inequalities, and a trickle– down theory, according to which more women capitalists and more women in the ruling class will empower the women in the working class.
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Categorising Network Telescope data using big data enrichment techniques

- Davis, Michael Reginald

Authors: Davis, Michael Reginald
Date: 2019
Subjects: Denial of service attacks , Big data , Computer networks -- Security measures
Language: English
Type: text , Thesis , Masters , MSc
Identifier: http://hdl.handle.net/10962/92941 , vital:30766
Description: Network Telescopes, Internet backbone sampling, IDS and other forms of network-sourced Threat Intelligence provide researchers with insight into the methods and intent of remote entities by capturing network traffic and analysing the resulting data. This analysis and determination of intent is made difficult by the large amounts of potentially malicious traffic, coupled with limited amount of knowledge that can be attributed to the source of the incoming data, as the source is known only by its IP address. Due to the lack of commonly available tooling, many researchers start this analysis from the beginning and so repeat and re-iterate previous research as the bulk of their work. As a result new insight into methods and approaches of analysis is gained at a high cost. Our research approaches this problem by using additional knowledge about the source IP address such as open ports, reverse and forward DNS, BGP routing tables and more, to enhance the researcher's ability to understand the traffic source. The research is a BigData experiment, where large (hundreds of GB) datasets are merged with a two month section of Network Telescope data using a set of Python scripts. The result are written to a Google BigQuery database table. Analysis of the network data is greatly simplified, with questions about the nature of the source, such as its device class (home routing device or server), potential vulnerabilities (open telnet ports or databases) and location becoming relatively easy to answer. Using this approach, researchers can focus on the questions that need answering and efficiently address them. This research could be taken further by using additional data sources such as Geo-location, WHOIS lookups, Threat Intelligence feeds and many others. Other potential areas of research include real-time categorisation of incoming packets, in order to better inform alerting and reporting systems' configuration. In conclusion, categorising Network Telescope data in this way provides insight into the intent of the (apparent) originator and as such is a valuable tool for those seeking to understand the purpose and intent of arriving packets. In particular, the ability to remove packets categorised as non-malicious (e.g. those in the Research category) from the data eliminates a known source of `noise' from the data. This allows the researcher to focus their efforts in a more productive manner.
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Centralising a counter public: an ethnographic study of the interpretation of mainstream news media by young adults in Joza

- Ponono, Mvuzo

Authors: Ponono, Mvuzo
Date: 2019
Subjects: African National Congress Mass media -- Political aspects -- South Africa Press and politics -- South Africa , South Africa -- Politics and government -- 21st century Zuma, Jacob -- Political and social views , Political corruption -- 21st century Elections -- South Africa Voting -- South Africa Misconduct in office -- South Africa Abuse of administrative power -- South Africa Young adults -- Political activity -- South Africa -- Grahamstown Young adults -- Politics and government
Language: English
Type: text , Thesis , Doctoral , PhD
Identifier: http://hdl.handle.net/10962/65033 , vital:28655
Description: The 2014 national general elections were characterised by a cloud of scandal hanging over the ANC, and the ANC president Jacob Zuma. The biggest and darkest cloud was the Nkandla scandal. Owing to a reported R246 million spent by the state to refurbish his private home, the president stood accused of wasteful expenditure and financial irregularity. In a country reeling from the continued effects of apartheid, which include high unemployment and poverty, the scandal was a bombshell. According to a vocal and often adversarial mainstream media sphere, the ANC went into those elections with an albatross around its neck. The dominant thought was that the ruling party would suffer a heavy loss of votes. This outcome did not materialise. The ANC lost a marginal share of its previous vote. Mainstream media and civil society were confounded. What had happened? Why had poor black South Africans continued to vote for a party that was obviously in breach of the constitutional order? Against the mismatch between what was predicted or purported and the outcome, this study investigates how young people in the township of Joza, Grahamstown, interpreted one of the biggest political scandals in South Africa’s fledgling democracy. Using a combination of subaltern studies, counter public sphere and audience study, the research looks into the interpretation of a mainstream media scandal that was supposed to diminish the chances of the ANC retaining power, but, instead, barely dented its majority. Through a combination of interviews and participant observation, the study found that young people in the township of Joza demonstrated that they chose to ignore the messages about the corruption of the ANC. The data suggests that they did so, not because of overt racial solidarity, but due to the fact that in a context of high inequality, and continued limitations on economic emancipation, the party shone brightly as a vehicle for economic development. Overall, the study argues that the seemingly dubious undertaking to continue with the ANC is a calculated decision that makes sense when viewed within a given socio-economic context.
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Changes and continuities over time in the cultural significance of the Nyaminyami water spirit among the BaTonga people of northwestern Zimbabwe

- Matanzima, Joshua

Authors: Matanzima, Joshua
Date: 2019
Subjects: Water spirits -- Zimbabwe , Tsonga (African people) -- Zimbabwe , Tsonga (African people) -- Zimbabwe -- Religion , Tsonga (African people) -- Zimbabwe -- Rites and ceremonies , Tsonga (African people) -- Zimbabwe -- Social life and customs , Mythology, Tsonga , Mythology, Zimbabwean , Nyaminyami (Spirit)
Language: English
Type: text , Thesis , Masters , MA
Identifier: http://hdl.handle.net/10962/94189 , vital:31015
Description: Research attests that beliefs in water spirits are an integral part of cultures of many indigenous communities across the globe. These water spirits play significant political, religious and socio-economic roles for the people concerned. However, the functions of water spirits are not constant, but change over time, especially when the people believing in water spirits undergo drastic socio- economic processes of change. It is in this context that this thesis traces the cultural significance over time, of the Nyaminyami water spirit, among some BaTonga people, living in the immediate vicinity of the Kariba gorge area, in north-western Zimbabwe. While previous studies document the existence of beliefs in Nyaminyami, none of these has systematically traced the historical significance of Nyaminyami, in terms of changes and continuities over time. Thus, this thesis makes a valuable contribution to knowledge with regards to the history and religion of the BaTonga people. The thesis argues that Nyaminyami‘s cultural significance or functions evolved over time, due to numerous socio- economic and political processes of change. The major changes that significantly influenced the practices relating to Nyaminyami include colonialism, Kariba dam construction and resettlement, the migration after resettlement in the 1960s and 1970s, the independence of Zimbabwe, and the alienation of the Kariba waterscape from the BaTonga. To be able to arrive at specific findings and conclusions, the thesis is underpinned by theories about resettlement, approaches to water divinities, and theories of religion and social change. The thesis has five ethnographic chapters that focus on specific time periods, illustrating the major socio- economic changes of each epoch, and showing how these changes impacted upon practices and beliefs relating to Nyaminyami. The thesis also documents how Nyaminyami beliefs are variedly distributed along different social variables that include gender, age, income and geographical location. In order to achieve the findings presented, the thesis utilized ethnographic evidence obtained from semi- structured interviews, participant observation, anthropology of extraordinary experience, document review and archival research.
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Changes in household use and sale of locally collected environmental resources over a 15-year period in a rural village, South Africa

- Falayi, Menelisi, Shackleton, Sheona E, Kemp, Georgina Cundill, Shackleton, Charlie M

Authors: Falayi, Menelisi , Shackleton, Sheona E , Kemp, Georgina Cundill , Shackleton, Charlie M
Date: 2019
Subjects: To be catalogued
Language: English
Type: text , article
Identifier: http://hdl.handle.net/10962/398196 , vital:69383 , xlink:href="https://doi.org/10.1080/14728028.2019.1568309"
Description: Many rural areas are experiencing rapid social-ecological changes, impacting livelihoods and creating less certain futures. Despite several decades of research on the use of locally collected environmental resources there have been few repeat studies, especially in South and Southern Africa, to consider how households may be altering such use alongside other changes. This paper explores changes in the use of environmental resources by communities in the Kat River Valley, South Africa over a fifteen-year period, between 2000 and 2015, using a repeat survey, key informant interviews and other data from a larger study relating to social-ecological change. The proportion of households collecting different environmental resources was similar between the two periods. However, the mean quantities used per household had declined for several resources . Consequently, the monetary contribution or worth of these to livelihoods also declined, except for fuelwood and river sand. These changes can be related to, amongst others, transformations in both the local landscape and socio-economic environment, such as increases in state social welfare, a decline in agricultural production, woody plant encroachment, generational preferences, and the expansion of supermarket retailers. Understanding such trends is important for considering the sustainable management of landscapes and livelihoods into the future.
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Characterization and computational studies of 2-(benzamido) thiazol-5-yl benzoate

- Odame, Felix, Hosten, Eric C, Betz, Richard, Lobb, Kevin A, Tshentu, Zenixole R

Authors: Odame, Felix , Hosten, Eric C , Betz, Richard , Lobb, Kevin A , Tshentu, Zenixole R
Date: 2019
Subjects: To be catalogued
Language: English
Type: text , article
Identifier: http://hdl.handle.net/10962/447003 , vital:74576 , xlink:href="https://doi.org/10.1134/S0022476619010190"
Description: Thiazoles have shown a broad range of biological activities and are found in many potent biologically active molecules such as Sulfathiazol (antimicrobial drug), Ritonavir (antiretroviral drug), Abafungin (antifungal drug), and Tiazofurin (antineoplastic drug) [1]. They have exhibited some degree of plant growth regulatory and antifungal activities [2], whilst some thiazoles have shown anti-infective [3] as well as antibacterial activities [4]. The regio-controlled synthesis of 2,5-disubstituted and 2,4,5-trisubstituted thiazoles from ethyl-2-bromo-5-chloro-4-thiazolecarboxylates using sequential palladium-catalyzed coupling reactions has been reported [5]. An efficient general method for the preparation of 2,4-di- and trisubsituted thiazoles is via P–TsOH. H2O-Catalyzed cyclization of trisubstituted propargylic alcohols with thioamides has been accomplished with moderate to excellent product yields under mild and standard conditions [6]. In the presence of triethylamine, (Z)-(2-acetoxyl-1-alkenyl) phenyl-λ3 iodanes reacts with thioureas or thioamides in methanol to afford 2,4- disubstituted thiazoles in good yields. The reaction is thought to proceed by the generation of highly reactive α-λ3 iodanyl ketones through ester exchange of the β-acetoxy group with liberation of methyl acetate, followed by nucleophilic substitutions with thioureas or thioamides [7].
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