A comprehensive review of the taxonomic diversity within the freshwater catfish genus Parauchenoglanis (Siluriformes, Auchenoglanididae)
- Authors: Sithole, Yonela
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/402992 , vital:69912
- Description: Thesis embargoed. To be released early 2026. , Thesis (PhD) -- Faculty of Science, Ichthyology & Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Sithole, Yonela
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/402992 , vital:69912
- Description: Thesis embargoed. To be released early 2026. , Thesis (PhD) -- Faculty of Science, Ichthyology & Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
A council of women
- Authors: Sobekwa, Lelethu Anathi
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435988 , vital:73218
- Description: This thesis comprises extracts of life writing written in short prose forms. The work reads like a novella and I have been inspired by Margaret Patton Chapman’s approach to the novella, where she condenses “the telling of a long story time wise”, so that a story occurring over two years can be told over two pages. The thesis explores relationships between mother, daughter, grandmother and granddaughter, each with different life experiences and each teaching the next generation about how to navigate life as politically, socially and economically disadvantaged women. I have also drawn inspiration from authors such as NoViolet Bulawayo who explores the hypocrisies of the church and the government in We Need New Names. In Kate Bernheimer’s “Fairy Tale is Form, Form is Fairy Tale” she writes about fairy tales adopting “intuitive logic” or telling in the form of “this happens and then this happens” while the explanation behind the events is not spelt out but rather exists between the lines. My thesis adopts this style of writing by allowing the reader to understand what is being said without over-simplifying. To this end, I have used the concept of place modelled on Es’kia Mphahlele’s In Corner B, where characters are inscribed in relation to the spaces they inhabit. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Sobekwa, Lelethu Anathi
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435988 , vital:73218
- Description: This thesis comprises extracts of life writing written in short prose forms. The work reads like a novella and I have been inspired by Margaret Patton Chapman’s approach to the novella, where she condenses “the telling of a long story time wise”, so that a story occurring over two years can be told over two pages. The thesis explores relationships between mother, daughter, grandmother and granddaughter, each with different life experiences and each teaching the next generation about how to navigate life as politically, socially and economically disadvantaged women. I have also drawn inspiration from authors such as NoViolet Bulawayo who explores the hypocrisies of the church and the government in We Need New Names. In Kate Bernheimer’s “Fairy Tale is Form, Form is Fairy Tale” she writes about fairy tales adopting “intuitive logic” or telling in the form of “this happens and then this happens” while the explanation behind the events is not spelt out but rather exists between the lines. My thesis adopts this style of writing by allowing the reader to understand what is being said without over-simplifying. To this end, I have used the concept of place modelled on Es’kia Mphahlele’s In Corner B, where characters are inscribed in relation to the spaces they inhabit. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2023
- Full Text:
- Date Issued: 2023-10-13
A molecular investigation of stem-galling Tetramesa Walker (Hymenoptera: Eurytomidae) on African grasses: applications to biological control
- Van Steenderen, Clarke Julian Mignon
- Authors: Van Steenderen, Clarke Julian Mignon
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432565 , vital:72881 , DOI 10.21504/10962/432565
- Description: South Africa is a larger donor than receiver of alien grasses, where approximately 15% (_ 165 spp.) of the country's native grass species have become naturalised elsewhere. Many of these grasses have become serious invaders, causing significant damage to native species, habitat structure, and ecosystem functioning. Biological control is a sustainable and cost-effective method for the control of invasive weeds, but its application to invasive grasses has been approached with trepidation in the past due to the fears of a lack of host-specific herbivores that may cause non-target damage to agriculturally-important crops. The Tetramesa Walker genus (Hymenoptera: Eurytomidae) is one of three genera in the family that feed exclusively on grasses, and have a record of being host-specific to a particular species, or complex of closely-related congeners. There are over 200 described Tetramesa species, but this taxonomic effort has occurred almost exclusively in the Northern Hemisphere. Only about 2% of the described species are from Africa, with none from southern Africa despite the high diversity of grasses in the region. The low morphological variability between many Tetramesa groups has made identification difficult, where there may in fact be multiple undiscovered cryptic species. This thesis generated genetic sequence data (mitochondrial COI and nuclear 28S) that revealed at least eight native southern African Tetramesa taxa that are new to science, focusing particularly on the assemblages associated with Eragrostis curvula Nees (African lovegrass) and Sporobolus pyramidalis Beauv. and S. natalensis Steud. (giant rat's tail grass) which are alien invasive pests in Australia. Approximately 200 eurytomid wasps were collected and sequenced from 19 grass species across six South African provinces. Additionally, 27 grass species were sequenced using four gene regions (rps16-trnK, rps16, rpl32-trnL, and ITS), which were added to existing sequence data to build a dataset comprising over 700 sequences. Field host ranges and the use of host grass genetic proxies were important in making inferences about the host-specificity of eurytomid wasps of interest. Nine Tetramesa groups appeared to be host-specific to a single grass species, while six Tetramesa were associated with multiple species in a single grass genus. Since S. pyramidalis, S. natalensis, S. africanus, Hyparrhenia hirta, E. trichophora, and Andropogon gayanus are weeds elsewhere, there are at least six potential Tetramesa biological control agents that have been identified. A high diversity of Tetramesa on grasses within the Eragrostis genus was reported, with at least four taxa associated with E. curvula. It is currently uncertain whether these taxa represent different cryptic species or intraspecific populations that are the result of geographic sub-structuring. No-choice host-specificity testing using Tetramesa sp. 4 on E. curvula revealed that the wasp could complete its lifecycle on two non-target African grasses; namely E. plana and E. planiculmis. The wasp was also recorded on other Eragrostis species in the field (namely E. biflora and E. capensis). Using grass genetic sequences obtained in this study, it was found that there are four native Australian Eragrostis species that are more closely related to target E. curvula than to the non-target E. plana and E. planiculmis. This suggests that Tetramesa sp. 4 may not be suitably host-specific for use as a biological control agent. Further host-specificity testing on these native Australian species is required, however, before this insect is ruled out completely. The Tetramesa on S. pyramidalis (Tetramesa sp. 1), and the unidentified Sporobolus species presumed to be S. africanus, were suitably host-specific to be used as biological control agents. Since it was unclear whether some phylogenetic clades were true species or intraspecific populations, which is essential to understand when selecting agents for biological control, a new piece of software, SPEDE-sampler", was developed. It offers users of the Generalised Mixed Yule Coalescent (GMYC) species delimitation model a means of assessing the degree to which sampling effects such as data size and parameter choice can influence species diversity estimates. When applied to the Tetramesa data set, the software assisted in identifying which groups may contain cryptic species, uncovering that the COI marker is affected more by singletons than the 28S marker (i.e. species diversity tends to be overestimated), and confirming putative Tetramesa taxa that could be useful for biological control programmes going forward. This thesis has provided evidence that South Africa contains a diverse assemblage of Tetramesa and other eurytomids that are closely associated with their grass hosts, and that many of these taxa hold promise for grass biological control. This work has also highlighted the importance of integrative taxonomy in the discovery of novel taxa, and that biological control practitioners need to be aware of the caveats of each line of evidence used in the delimitation of putative species. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Van Steenderen, Clarke Julian Mignon
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432565 , vital:72881 , DOI 10.21504/10962/432565
- Description: South Africa is a larger donor than receiver of alien grasses, where approximately 15% (_ 165 spp.) of the country's native grass species have become naturalised elsewhere. Many of these grasses have become serious invaders, causing significant damage to native species, habitat structure, and ecosystem functioning. Biological control is a sustainable and cost-effective method for the control of invasive weeds, but its application to invasive grasses has been approached with trepidation in the past due to the fears of a lack of host-specific herbivores that may cause non-target damage to agriculturally-important crops. The Tetramesa Walker genus (Hymenoptera: Eurytomidae) is one of three genera in the family that feed exclusively on grasses, and have a record of being host-specific to a particular species, or complex of closely-related congeners. There are over 200 described Tetramesa species, but this taxonomic effort has occurred almost exclusively in the Northern Hemisphere. Only about 2% of the described species are from Africa, with none from southern Africa despite the high diversity of grasses in the region. The low morphological variability between many Tetramesa groups has made identification difficult, where there may in fact be multiple undiscovered cryptic species. This thesis generated genetic sequence data (mitochondrial COI and nuclear 28S) that revealed at least eight native southern African Tetramesa taxa that are new to science, focusing particularly on the assemblages associated with Eragrostis curvula Nees (African lovegrass) and Sporobolus pyramidalis Beauv. and S. natalensis Steud. (giant rat's tail grass) which are alien invasive pests in Australia. Approximately 200 eurytomid wasps were collected and sequenced from 19 grass species across six South African provinces. Additionally, 27 grass species were sequenced using four gene regions (rps16-trnK, rps16, rpl32-trnL, and ITS), which were added to existing sequence data to build a dataset comprising over 700 sequences. Field host ranges and the use of host grass genetic proxies were important in making inferences about the host-specificity of eurytomid wasps of interest. Nine Tetramesa groups appeared to be host-specific to a single grass species, while six Tetramesa were associated with multiple species in a single grass genus. Since S. pyramidalis, S. natalensis, S. africanus, Hyparrhenia hirta, E. trichophora, and Andropogon gayanus are weeds elsewhere, there are at least six potential Tetramesa biological control agents that have been identified. A high diversity of Tetramesa on grasses within the Eragrostis genus was reported, with at least four taxa associated with E. curvula. It is currently uncertain whether these taxa represent different cryptic species or intraspecific populations that are the result of geographic sub-structuring. No-choice host-specificity testing using Tetramesa sp. 4 on E. curvula revealed that the wasp could complete its lifecycle on two non-target African grasses; namely E. plana and E. planiculmis. The wasp was also recorded on other Eragrostis species in the field (namely E. biflora and E. capensis). Using grass genetic sequences obtained in this study, it was found that there are four native Australian Eragrostis species that are more closely related to target E. curvula than to the non-target E. plana and E. planiculmis. This suggests that Tetramesa sp. 4 may not be suitably host-specific for use as a biological control agent. Further host-specificity testing on these native Australian species is required, however, before this insect is ruled out completely. The Tetramesa on S. pyramidalis (Tetramesa sp. 1), and the unidentified Sporobolus species presumed to be S. africanus, were suitably host-specific to be used as biological control agents. Since it was unclear whether some phylogenetic clades were true species or intraspecific populations, which is essential to understand when selecting agents for biological control, a new piece of software, SPEDE-sampler", was developed. It offers users of the Generalised Mixed Yule Coalescent (GMYC) species delimitation model a means of assessing the degree to which sampling effects such as data size and parameter choice can influence species diversity estimates. When applied to the Tetramesa data set, the software assisted in identifying which groups may contain cryptic species, uncovering that the COI marker is affected more by singletons than the 28S marker (i.e. species diversity tends to be overestimated), and confirming putative Tetramesa taxa that could be useful for biological control programmes going forward. This thesis has provided evidence that South Africa contains a diverse assemblage of Tetramesa and other eurytomids that are closely associated with their grass hosts, and that many of these taxa hold promise for grass biological control. This work has also highlighted the importance of integrative taxonomy in the discovery of novel taxa, and that biological control practitioners need to be aware of the caveats of each line of evidence used in the delimitation of putative species. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
A study on visual inspection of citrus sorters in a packhouse in the Eastern Cape of South Africa
- Authors: Maher, Christopher Richard
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424256 , vital:72137
- Description: Background and Purpose. The South African citrus industry is a major global producer of citrus as well as the second largest exporter of citrus fruit in the world. This industry provides employment to an estimated 112 000 workers in South Africa and is vital in providing economic opportunities to communities such as those in the Sunday’s River Valley in the Eastern Cape province of South Africa due to the low employment and education levels within this province. Citrus sorting is a visual inspection task that requires workers to sustain attention and remain vigilant for the duration of a working shift. Their role in the packhouse is to ensure that no defective products are packed for export or the local market and that the fruit meets the customers’ expectations. Since the demands of sorting citrus are poorly understood, this study attempted to assess the effect that sorting citrus has on vigilance performance as well as attempt to quantify perceived workload while taking into account different contextual factors that affect perceptions of this working task. Methods. This study was conducted within a citrus packhouse in the Eastern Cape province of South Africa. The sample was comprised of individuals employed by the packhouse as citrus sorters. In addition to a recruitment and information session, testing was conducted at three intervals during a working shift on four consecutive days. Each time, indicators of vigilance, such as correct responses, sensitivity, reaction time, false alarms and misses, were collected during a 5-minute computerised version of the Mackworth Clock-test, while perceived workload was assessed with the NASA-TLX scale, and sleepiness was recording using the WITS Sleepiness Scale. Furthermore, an open-ended question was asked to further understand factors affecting the ability of participants to concentrate during their task of sorting. Results. 18 participants consented to participate in this study. 16 of these individuals identified as female with an average age of 30 years (± 7). 83% of participants were of Black African descent and were isiXhosa speakers, while 17% of participants were of Coloured descent and Afrikaans speakers. This study found that performance, as measured by the percentage of correct responses, sensitivity and reaction time on the 5-minute long Mackworth Clock Test was not significantly affected as time-on-task for sorting citrus increased during a working shift, neither did performance on the vigilance test change as days of the week proceeded. However, ‘misses’ during the vigilance test were found to significantly decrease over the course of a working shift, thus contradicting the expected outcomes of this study. Perceived workload of sorting citrus, as well as perceived sleepiness, significantly increased with time-on-task during the course of a working shift. The major contributions for this increase in perceived workload while sorting citrus were derived from the Mental Demand and Physical Demand sub-scale for the NASA-TLX. However, responses from the open-ended question indicated that participants generally enjoyed their work, although there were factors, such as the nature of fruit and social set-up of work, that negatively affected this perception. Discussion. The results from this study contradicted the researcher’s expectations. It was anticipated that measures of correct responses and sensitivity would decrease and misses, and reaction time would increase with time-on-task for sorting citrus, yet vigilance indicators from the Mackworth Clock Test generally indicated no change in vigilance, and, according to “misses” even improved with time-on-task. A likely explanation for this is that the use of a 5-minute Mackworth Clock-test was unsuitable in this context, or rather, the time of this test was not long enough to induce a ‘vigilance decrement’. Furthermore, in order to administer this vigilance test, participants were taken off the production line and led to a testing room which most likely increased their levels of arousal, thus affecting performance on the vigilance test. However, even though perceived sleepiness was found to significantly increase over the course of a working shift, these data correlated poorly with the vigilance results, thus contradicting the expected outcomes of the study. Mental demand significantly increased, possibly due to the demand of attentional informational processing required for sorting, as well as various environmental factors present within the packhouse. Physical demand, too, significantly increased as workers were required to remain standing throughout their working task as well as to make use of their upper extremities to remove defective fruit. Correlation analyses showed significantly weak correlation for Mackworth Clock Test variables and sleepiness ratings to all other variables. NASA-TLX variables, however, generally showed significantly moderate correlations with one another. Conclusion. It remains unclear how vigilance changes and sustained attention while sorting citrus, since the experiment did not show changes in performance. This is largely attributed to the methodological set-up which could have increased arousal and therefore affected vigilance. It is recommended that future studies research vigilance and sustained attention during the sorting itself, rather than attempting to infer vigilance results from a computerised vigilance test. Perceived workload of sorting, however, increased over the course of a working shift due to significant increases in perceptions of the physical and mental demands of sorting. Future studies should address concerns relating to the physical demand of sorting. Furthermore, it would be beneficial to compare responses of day and night shift sorters to further understand how the relationship between these variables may change. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Maher, Christopher Richard
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424256 , vital:72137
- Description: Background and Purpose. The South African citrus industry is a major global producer of citrus as well as the second largest exporter of citrus fruit in the world. This industry provides employment to an estimated 112 000 workers in South Africa and is vital in providing economic opportunities to communities such as those in the Sunday’s River Valley in the Eastern Cape province of South Africa due to the low employment and education levels within this province. Citrus sorting is a visual inspection task that requires workers to sustain attention and remain vigilant for the duration of a working shift. Their role in the packhouse is to ensure that no defective products are packed for export or the local market and that the fruit meets the customers’ expectations. Since the demands of sorting citrus are poorly understood, this study attempted to assess the effect that sorting citrus has on vigilance performance as well as attempt to quantify perceived workload while taking into account different contextual factors that affect perceptions of this working task. Methods. This study was conducted within a citrus packhouse in the Eastern Cape province of South Africa. The sample was comprised of individuals employed by the packhouse as citrus sorters. In addition to a recruitment and information session, testing was conducted at three intervals during a working shift on four consecutive days. Each time, indicators of vigilance, such as correct responses, sensitivity, reaction time, false alarms and misses, were collected during a 5-minute computerised version of the Mackworth Clock-test, while perceived workload was assessed with the NASA-TLX scale, and sleepiness was recording using the WITS Sleepiness Scale. Furthermore, an open-ended question was asked to further understand factors affecting the ability of participants to concentrate during their task of sorting. Results. 18 participants consented to participate in this study. 16 of these individuals identified as female with an average age of 30 years (± 7). 83% of participants were of Black African descent and were isiXhosa speakers, while 17% of participants were of Coloured descent and Afrikaans speakers. This study found that performance, as measured by the percentage of correct responses, sensitivity and reaction time on the 5-minute long Mackworth Clock Test was not significantly affected as time-on-task for sorting citrus increased during a working shift, neither did performance on the vigilance test change as days of the week proceeded. However, ‘misses’ during the vigilance test were found to significantly decrease over the course of a working shift, thus contradicting the expected outcomes of this study. Perceived workload of sorting citrus, as well as perceived sleepiness, significantly increased with time-on-task during the course of a working shift. The major contributions for this increase in perceived workload while sorting citrus were derived from the Mental Demand and Physical Demand sub-scale for the NASA-TLX. However, responses from the open-ended question indicated that participants generally enjoyed their work, although there were factors, such as the nature of fruit and social set-up of work, that negatively affected this perception. Discussion. The results from this study contradicted the researcher’s expectations. It was anticipated that measures of correct responses and sensitivity would decrease and misses, and reaction time would increase with time-on-task for sorting citrus, yet vigilance indicators from the Mackworth Clock Test generally indicated no change in vigilance, and, according to “misses” even improved with time-on-task. A likely explanation for this is that the use of a 5-minute Mackworth Clock-test was unsuitable in this context, or rather, the time of this test was not long enough to induce a ‘vigilance decrement’. Furthermore, in order to administer this vigilance test, participants were taken off the production line and led to a testing room which most likely increased their levels of arousal, thus affecting performance on the vigilance test. However, even though perceived sleepiness was found to significantly increase over the course of a working shift, these data correlated poorly with the vigilance results, thus contradicting the expected outcomes of the study. Mental demand significantly increased, possibly due to the demand of attentional informational processing required for sorting, as well as various environmental factors present within the packhouse. Physical demand, too, significantly increased as workers were required to remain standing throughout their working task as well as to make use of their upper extremities to remove defective fruit. Correlation analyses showed significantly weak correlation for Mackworth Clock Test variables and sleepiness ratings to all other variables. NASA-TLX variables, however, generally showed significantly moderate correlations with one another. Conclusion. It remains unclear how vigilance changes and sustained attention while sorting citrus, since the experiment did not show changes in performance. This is largely attributed to the methodological set-up which could have increased arousal and therefore affected vigilance. It is recommended that future studies research vigilance and sustained attention during the sorting itself, rather than attempting to infer vigilance results from a computerised vigilance test. Perceived workload of sorting, however, increased over the course of a working shift due to significant increases in perceptions of the physical and mental demands of sorting. Future studies should address concerns relating to the physical demand of sorting. Furthermore, it would be beneficial to compare responses of day and night shift sorters to further understand how the relationship between these variables may change. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2023
- Full Text:
- Date Issued: 2023-10-13
African linguistic phenomenology as illustrated through the Setswana language
- Authors: Modisakeng, Lemogang
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/425089 , vital:72208
- Description: Embargoed. Expected release date 2025. , Thesis (MA) -- Faculty of Humanities, Philosophy, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Modisakeng, Lemogang
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/425089 , vital:72208
- Description: Embargoed. Expected release date 2025. , Thesis (MA) -- Faculty of Humanities, Philosophy, 2023
- Full Text:
- Date Issued: 2023-10-13
African population prevalent genetic variations of dihydropyrimidine dehydrogenase as the 5-flourouracil cancer drug metabolizing enzyme: computational approaches towards pharmacogenomics studies
- Authors: Tendwa, Maureen Bilinga
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432263 , vital:72856 , DOI 10.21504/10962/432270
- Description: In an era of newly emerging cases of non-communicable diseases such as cancer, research is vital for both the medical and economic well-being of humanity. Pharmacogenomics has laidthegroundworkfor the identification of potential genes in cancer progression and treatment outcome investigations. Researchers are increasingly discovering heterogeneity in the efficacy and toxicity responses of drugmetabolizing enzymes (DMEs) in diverse patient populations receiving anti-cancer therapy. DMEs comprise of Phase I (Cytochrome P450s) and Phase II (glutathione-S-transferases (GSTs), UDP-glucuronosyltransferases (UGTs), and dihydropyrimidine dehydrogenases (DPD)enzymes. The main cause of disparity in DME treatment outcomes is genetic variation,which causes missense mutations leading to structural and kinetic properties of the enzyme. These modifications have a deleterious impact on the pharmacodynamics and pharmacokinetics of drugs through multiple mechanisms. Presently, most cancer medicines are manufacturedin developed countries based on the genetic background of non-African subpopulations. Thus, these drugs may not be optimally effective or can cause adverse side effects. Even though heterogeneity in toxicity and efficacy of these drugs has been observed in African descent, the basis of this population variance remains partially understood. For instance,a deficiencyof DPD, the first-rate limiting metabolizing enzyme in the pyrimidinepathway, causes severe toxicity when exposed to 5-fluorouracil (5-FU) chemotherapy. However, minimum studies have been conducted to unravel itsmolecular mechanismwhich may unravel the observed drug treatment outcomes.The aim of this pharmacogenomics study was to determine the underlying mechanism by which DPD missense mutations, which are associated with an African ancestry subpopulation, provoke dysfunctional 5-FU metabolism, resulting in drug toxicity. This knowledge will be critical in designing drug modulators to aid in the restoration of DPD function, a hallmark of precision medicine. Therefore, in the first part of the research we identified and reviewed the general role of Phase I and Phase II cancer drug metabolizing enzymes. We then used World Health Organization (WHO) essential medicine and drug.com to authenticate the usage of 5-FU as an anti-cancer treatment agent. The 3D structure and chemical structure of the agent was then downloaded from the Drug bank. Subsequently, Human Mutation Analysis - Variant Analysis PORtal (HUMA) and Mendelian Inheritance in Man (OMIM) were used to obtain data on DPD non-synonymous genetic variants. Additionally, the aggregate information of DPD missense mutations and their relation to human health were extracted from ClinVar and Pharmacogenomics Knowledge Base (PharmKGB). This information, along with additional data from single nucleotide polymorphisms (dbSNP), 1000 Genomes Project and Exome Sequencing Project (ESP MAF) considering variants classified based on their minor allele frequency (MAF) of 0.001, as well as research articles, consolidated information on missense mutations associated with African subpopulations. Finally, the wild type (WT) and detected mutation sequences were obtained from the Universal Protein Resources database (UniProt). However, because the 3D structure of human DPD was missing, the dimeric wild type (WT) human 3-dimensional (3D) structure was modeled via MODELLER using the pig’s structure as a template. PRIMO, HHpred, and the Protein Data Bank (PDB) were all used to locate the suitable template. As a result, six clinical (C29R, M166V, Y186C, S534N, I543V, and D949V) and thirteen non-clinical (S201R, K259E, D342N, D432N, S492L, R592Q, A664S, G674D, A721T, V732G, T768K, R886C, and L993R) mutations were discovered. Using AMBER tools, we then determined accurate force field parameters for each monomer of DPD protein's Fe2+ centers. Following the creation of each mutation model structure in Discovery Studio, the resulting AMBER force field parameters were inferred. For each model structure, a drug free (inactive/open-conformation) and drug bound (active/closed-conformation) model structure was created (WT and mutations). The model structures were validated using the consensus of three validation programs, namely ERRAT, PROCHECK, and ProSA. Similarly, the impact on structural functionalities was predicted by consensus from Variant Analysis Porta (VAPOR) web server, which include three support vector machines (SVM)-based tools; PhD-SNP, MUpro, and I-Mutation. After protonation in the H++ web server, the six clinical and thirteen non-clinical (six active site and seven non-active site) mutations identified were then exposed to 600 ns molecular dynamic (MD) simulation. The non-clinical data was divided into two categories to better understand the impact of the mutation based on its position in the protein: six catalytic-domain (R592Q, A664S, G674D, A721T, V732G, and T768K) and seven remote (S201R, K259E, D342N, D432N, S492L, R886C, and L993R) missense mutations. The post-MD analysis was done using the typical existing computational global investigations [RMSD, all versus all RMSD, RMSF, RG, hydrogen bonds (H-bonds) and dynamic cross correlation (DCC)]. In addition, we used in silico tools newly developed within the Research Unit in Bioinformatics (RUBI) group, such as comparative essential dynamics (ED)-principal component analysis and dynamic residue network (DRN) multi-metric [betweenness centrality (BC), closeness centrality (CC), degree of centrality (DC), eigen-centrality (EC) and Katz centrality (KC)] analysis algorithms. From the analysis, it was observed that the loop regions of the mutation proteins had increased loop flexibility, particularly around the catalytic loop, which could account for the enhanced asymmetric behavior of the mutation’s monomers compared to the WT. Notably, the A664S mutant showed relatively lower fluctuations, deviating from the observed heightened flexibility in other mutants. A general decrease in hydrogen bonds was observed in the 5-FU binding environment of the mutations compared to the WT. In particular, 5-FU contact analysis of the WT versus the mutation revealed a reduction in contact between core 5-FU binding residues and catalytic residues Cys671 and Ser670, which form hydrogen bonds that initiate DPD catalytic action. Additionally, BC was used to quantify the importance of a protein residue based on how often it acted as a bridge along the shortest paths between other residues. It reflected the potential control or influence a residue may have over communication between different parts of a protein structure. DC assesses the number of connections or interactions a residue had with other residues in the protein, indicating its overall connectivity within the structure. In both drug free and drug bound state, DPD data from the active site hubs' BC and DC revealed a dimeric asymmetric communication pathway per monomer involving a cluster of newly introduced hubs ensemble along the oxidoreduction conduit from NADPH to 5-FU. The two BC communication pathways were located more on the interior of the oxidoreduction conduit, while the two DC communication pathways were located on the exterior. In both cases, one pathway dominated the other. Partially lost function reported in mutation systems could be credited to the compensation communication response to the catalytic site via the least compromised routes. Similar patterns were observed in allosteric communication pathways to the active site induced by remote mutations. Mutations may have destabilized the active-loop and 5-FU binding environment, resulting in a compensatory mechanism seen by the addition of new hubs to the communication network. Surprisingly, EC hubs in the WT were found within the catalytic site domain, indicating that the region is important in 5-FU metabolism. EC measured the importance of a residue by considering both its own degree of connectivity and the degrees of connectivity with its neighboring residues, highlighting its significance in information flow and communication. Herein, EC hubs in mutant systems were found to lose this importance, with active site domain mutations suffering the most. This could explain why non-clinical catalytic domain mutations R592Q, A664S, and G674D, as well as clinical catalytic domain mutations S534N and I543V, experienced drug exit in one of their monomers during simulation. In contrast, there was no 5-FU exit in the non-clinical remote domain. Additionally, aside from the active site, KC hubs were also found around the cofactors, indicating that these components were equally important in DPD overall function. KC combines the concepts of both degree centrality and eigen-centrality, it incorporated both direct and indirect interactions to evaluate the importance of a residue, assigning higher centrality to residues that have connections to other highly central residues. Hence, providing a more comprehensive measure of influence within the protein network. More importantly, CC is known to measure how efficiently a residue can interact with other residues in the protein, considering the shortest path lengths. It indicates the proximity of a residue to others, suggesting its potential for information transfer or functional integration. CC revealed that the majority of persistent hubs were found within the protein-cores known as cold-spots. Overall, this study highlighted the communication pathways triggered by active site domain mutations, as well as the allosteric communication pathways triggered by each remote mutation in both drug free and drug bound states of the DPD enzyme. Both clinical and non-clinical mutations revealed each protein's adaptive compensation mechanism, which results in partial function loss. In each case, the communication network of the different monomers changed from inactive to activated DPD protein. Cold-spot areas were discovered to contain key persistent residues involved in protein function and stability. These areas have been proposed as potential targets for new or repurposed pharmacological modulators that can restore enzyme function. In the pursuit of precision medicine, it also lays the groundwork for detecting and explaining the molecular mechanisms of other drug metabolizing enzymes related to the African-descent subpopulation. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Tendwa, Maureen Bilinga
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432263 , vital:72856 , DOI 10.21504/10962/432270
- Description: In an era of newly emerging cases of non-communicable diseases such as cancer, research is vital for both the medical and economic well-being of humanity. Pharmacogenomics has laidthegroundworkfor the identification of potential genes in cancer progression and treatment outcome investigations. Researchers are increasingly discovering heterogeneity in the efficacy and toxicity responses of drugmetabolizing enzymes (DMEs) in diverse patient populations receiving anti-cancer therapy. DMEs comprise of Phase I (Cytochrome P450s) and Phase II (glutathione-S-transferases (GSTs), UDP-glucuronosyltransferases (UGTs), and dihydropyrimidine dehydrogenases (DPD)enzymes. The main cause of disparity in DME treatment outcomes is genetic variation,which causes missense mutations leading to structural and kinetic properties of the enzyme. These modifications have a deleterious impact on the pharmacodynamics and pharmacokinetics of drugs through multiple mechanisms. Presently, most cancer medicines are manufacturedin developed countries based on the genetic background of non-African subpopulations. Thus, these drugs may not be optimally effective or can cause adverse side effects. Even though heterogeneity in toxicity and efficacy of these drugs has been observed in African descent, the basis of this population variance remains partially understood. For instance,a deficiencyof DPD, the first-rate limiting metabolizing enzyme in the pyrimidinepathway, causes severe toxicity when exposed to 5-fluorouracil (5-FU) chemotherapy. However, minimum studies have been conducted to unravel itsmolecular mechanismwhich may unravel the observed drug treatment outcomes.The aim of this pharmacogenomics study was to determine the underlying mechanism by which DPD missense mutations, which are associated with an African ancestry subpopulation, provoke dysfunctional 5-FU metabolism, resulting in drug toxicity. This knowledge will be critical in designing drug modulators to aid in the restoration of DPD function, a hallmark of precision medicine. Therefore, in the first part of the research we identified and reviewed the general role of Phase I and Phase II cancer drug metabolizing enzymes. We then used World Health Organization (WHO) essential medicine and drug.com to authenticate the usage of 5-FU as an anti-cancer treatment agent. The 3D structure and chemical structure of the agent was then downloaded from the Drug bank. Subsequently, Human Mutation Analysis - Variant Analysis PORtal (HUMA) and Mendelian Inheritance in Man (OMIM) were used to obtain data on DPD non-synonymous genetic variants. Additionally, the aggregate information of DPD missense mutations and their relation to human health were extracted from ClinVar and Pharmacogenomics Knowledge Base (PharmKGB). This information, along with additional data from single nucleotide polymorphisms (dbSNP), 1000 Genomes Project and Exome Sequencing Project (ESP MAF) considering variants classified based on their minor allele frequency (MAF) of 0.001, as well as research articles, consolidated information on missense mutations associated with African subpopulations. Finally, the wild type (WT) and detected mutation sequences were obtained from the Universal Protein Resources database (UniProt). However, because the 3D structure of human DPD was missing, the dimeric wild type (WT) human 3-dimensional (3D) structure was modeled via MODELLER using the pig’s structure as a template. PRIMO, HHpred, and the Protein Data Bank (PDB) were all used to locate the suitable template. As a result, six clinical (C29R, M166V, Y186C, S534N, I543V, and D949V) and thirteen non-clinical (S201R, K259E, D342N, D432N, S492L, R592Q, A664S, G674D, A721T, V732G, T768K, R886C, and L993R) mutations were discovered. Using AMBER tools, we then determined accurate force field parameters for each monomer of DPD protein's Fe2+ centers. Following the creation of each mutation model structure in Discovery Studio, the resulting AMBER force field parameters were inferred. For each model structure, a drug free (inactive/open-conformation) and drug bound (active/closed-conformation) model structure was created (WT and mutations). The model structures were validated using the consensus of three validation programs, namely ERRAT, PROCHECK, and ProSA. Similarly, the impact on structural functionalities was predicted by consensus from Variant Analysis Porta (VAPOR) web server, which include three support vector machines (SVM)-based tools; PhD-SNP, MUpro, and I-Mutation. After protonation in the H++ web server, the six clinical and thirteen non-clinical (six active site and seven non-active site) mutations identified were then exposed to 600 ns molecular dynamic (MD) simulation. The non-clinical data was divided into two categories to better understand the impact of the mutation based on its position in the protein: six catalytic-domain (R592Q, A664S, G674D, A721T, V732G, and T768K) and seven remote (S201R, K259E, D342N, D432N, S492L, R886C, and L993R) missense mutations. The post-MD analysis was done using the typical existing computational global investigations [RMSD, all versus all RMSD, RMSF, RG, hydrogen bonds (H-bonds) and dynamic cross correlation (DCC)]. In addition, we used in silico tools newly developed within the Research Unit in Bioinformatics (RUBI) group, such as comparative essential dynamics (ED)-principal component analysis and dynamic residue network (DRN) multi-metric [betweenness centrality (BC), closeness centrality (CC), degree of centrality (DC), eigen-centrality (EC) and Katz centrality (KC)] analysis algorithms. From the analysis, it was observed that the loop regions of the mutation proteins had increased loop flexibility, particularly around the catalytic loop, which could account for the enhanced asymmetric behavior of the mutation’s monomers compared to the WT. Notably, the A664S mutant showed relatively lower fluctuations, deviating from the observed heightened flexibility in other mutants. A general decrease in hydrogen bonds was observed in the 5-FU binding environment of the mutations compared to the WT. In particular, 5-FU contact analysis of the WT versus the mutation revealed a reduction in contact between core 5-FU binding residues and catalytic residues Cys671 and Ser670, which form hydrogen bonds that initiate DPD catalytic action. Additionally, BC was used to quantify the importance of a protein residue based on how often it acted as a bridge along the shortest paths between other residues. It reflected the potential control or influence a residue may have over communication between different parts of a protein structure. DC assesses the number of connections or interactions a residue had with other residues in the protein, indicating its overall connectivity within the structure. In both drug free and drug bound state, DPD data from the active site hubs' BC and DC revealed a dimeric asymmetric communication pathway per monomer involving a cluster of newly introduced hubs ensemble along the oxidoreduction conduit from NADPH to 5-FU. The two BC communication pathways were located more on the interior of the oxidoreduction conduit, while the two DC communication pathways were located on the exterior. In both cases, one pathway dominated the other. Partially lost function reported in mutation systems could be credited to the compensation communication response to the catalytic site via the least compromised routes. Similar patterns were observed in allosteric communication pathways to the active site induced by remote mutations. Mutations may have destabilized the active-loop and 5-FU binding environment, resulting in a compensatory mechanism seen by the addition of new hubs to the communication network. Surprisingly, EC hubs in the WT were found within the catalytic site domain, indicating that the region is important in 5-FU metabolism. EC measured the importance of a residue by considering both its own degree of connectivity and the degrees of connectivity with its neighboring residues, highlighting its significance in information flow and communication. Herein, EC hubs in mutant systems were found to lose this importance, with active site domain mutations suffering the most. This could explain why non-clinical catalytic domain mutations R592Q, A664S, and G674D, as well as clinical catalytic domain mutations S534N and I543V, experienced drug exit in one of their monomers during simulation. In contrast, there was no 5-FU exit in the non-clinical remote domain. Additionally, aside from the active site, KC hubs were also found around the cofactors, indicating that these components were equally important in DPD overall function. KC combines the concepts of both degree centrality and eigen-centrality, it incorporated both direct and indirect interactions to evaluate the importance of a residue, assigning higher centrality to residues that have connections to other highly central residues. Hence, providing a more comprehensive measure of influence within the protein network. More importantly, CC is known to measure how efficiently a residue can interact with other residues in the protein, considering the shortest path lengths. It indicates the proximity of a residue to others, suggesting its potential for information transfer or functional integration. CC revealed that the majority of persistent hubs were found within the protein-cores known as cold-spots. Overall, this study highlighted the communication pathways triggered by active site domain mutations, as well as the allosteric communication pathways triggered by each remote mutation in both drug free and drug bound states of the DPD enzyme. Both clinical and non-clinical mutations revealed each protein's adaptive compensation mechanism, which results in partial function loss. In each case, the communication network of the different monomers changed from inactive to activated DPD protein. Cold-spot areas were discovered to contain key persistent residues involved in protein function and stability. These areas have been proposed as potential targets for new or repurposed pharmacological modulators that can restore enzyme function. In the pursuit of precision medicine, it also lays the groundwork for detecting and explaining the molecular mechanisms of other drug metabolizing enzymes related to the African-descent subpopulation. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-10-13
An analysis of power relations, affiliation and individuation in selected coup, secession, and inaugural speeches of Nigerian leaders, 1960-2015
- Authors: Unegbu, Osondu Chukwuemeka
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432399 , vital:72867 , DOI 10.21504/10962/432399
- Description: This study examines the positioning of Zimbabwe and the United Kingdom (UK) in the language of news articles about diplomatic relations between the two countries published in two prominent Zimbabwean newspapers, The Herald and The Standard, between 2016 and 2020, using Systemic Functional Linguistics (SFL) and Legitimation Code Theory (LCT). Zimbabwe's present diplomatic discourses are a product of its colonial and post-independence history. This thesis places The Herald and The Standard within the larger context of the Zimbabwean media landscape as a state-owned and a privately owned newspaper, respectively. It contends that the two newspapers influence the positioning of Zimbabwe and the United Kingdom in public spheres where readers engage with the news concerning diplomatic ties. The research begins with a corpus-based examination of 42 news articles published between 2016 and 2020 in The Herald and The Standard. The investigation identified several angles through which Zimbabwe and the UK are positioned in the coverage. Zimbabwe is positioned through reference to its reform agenda, intentions for free and fair elections, and its diplomatic re-engagement drive. The UK is positioned as a source of development funding and new trade agreements, while improved diplomatic relations and the removal of restrictive measures against Zimbabwe are anticipated. Fine-grained analysis was conducted on four articles, two from each newspaper, selected to reflect the trends found in the corpus analysis. The fine-grained analyses showed how linguistic resources contributed to the positioning of the UK and Zimbabwe. Complementary analyses of these articles were conducted using LCT and SFL to describe how readers' knowledge of diplomatic relations is built using language in The Herald and The Standard. The study used the concepts of constellations and cosmologies from LCT to demonstrate how diplomatic knowledge is built in these news articles. In the articles, the constellations are related to policies such as Zimbabwe's reform agenda, people (diplomats and government officials) and moral judgments. Both newspapers contain both positive and negative positioning of Zimbabwe and the UK. However, The Standard is generally critical of removing the UK's sanctions on Zimbabwe and of the Zimbabwean government's reform agenda. Meanwhile, The Herald justifies Zimbabwe's Fast-track Land Reform Programme as resulting from Britain's refusal to fund a land redistribution programme as per the 1979 Lancaster House Agreement. The use of SFL's Appraisal framework helps to examine the linguistic resources used by The Herald and The Standard to describe the positioning of the two countries in relation to each other. Lexical strings help to establish diplomatic positioning in the news articles. These couple with Appraisal to accomplish individuation and affiliation. Affect and Judgement resources revealed the decisions taken by the UK to solve the conflict by encouraging the implementation of a reform agenda by Zimbabwe. Negative evaluation is consistently used to disalign with sanctions. The emerging patterns in the data show that diplomats representing the UK affiliate with Zimbabwe's stated economic and political reforms, and the government of Zimbabwe affiliates with the new trade agreement between Zimbabwe and the UK. At the same time, the UK diplomats individuate away from human rights abuses and the Fast-track Land Reform Programme. Affiliation strengthens confidence in diplomatic ties between Zimbabwe and the UK because the emphasis is placed on restoring them despite the conditions attached. The Herald and The Standard position Zimbabwe as a reformed country ready to implement policies to improve citizens' lives and as a country violating human rights and the rule of law. They position the UK as asserting power over Zimbabwe, willing to resolve the crisis through its foreign policy, and credited for assisting Zimbabwe. This strengthens re-engagement in diplomatic relations and commercial trade between the UK and Zimbabwe. In light of these findings, Zimbabweans are encouraged to cultivate an awareness that enables them to reflect on the challenges associated with diplomatic discourses and the implications for critically analysing the re-engagement initiative. They can promote re-engagement by being cognisant of specific values portrayed in The Herald and The Standard and challenging these values in the light of policy transformation to revive the relations between the two countries. The re-engagement process requires a transformation in Zimbabwe's modus operandi to improve the country's positioning in the diplomatic relations between it and the UK. , Thesis (PhD) -- Faculty of Humanities, Linguistics and Applied Language Studies, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Unegbu, Osondu Chukwuemeka
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432399 , vital:72867 , DOI 10.21504/10962/432399
- Description: This study examines the positioning of Zimbabwe and the United Kingdom (UK) in the language of news articles about diplomatic relations between the two countries published in two prominent Zimbabwean newspapers, The Herald and The Standard, between 2016 and 2020, using Systemic Functional Linguistics (SFL) and Legitimation Code Theory (LCT). Zimbabwe's present diplomatic discourses are a product of its colonial and post-independence history. This thesis places The Herald and The Standard within the larger context of the Zimbabwean media landscape as a state-owned and a privately owned newspaper, respectively. It contends that the two newspapers influence the positioning of Zimbabwe and the United Kingdom in public spheres where readers engage with the news concerning diplomatic ties. The research begins with a corpus-based examination of 42 news articles published between 2016 and 2020 in The Herald and The Standard. The investigation identified several angles through which Zimbabwe and the UK are positioned in the coverage. Zimbabwe is positioned through reference to its reform agenda, intentions for free and fair elections, and its diplomatic re-engagement drive. The UK is positioned as a source of development funding and new trade agreements, while improved diplomatic relations and the removal of restrictive measures against Zimbabwe are anticipated. Fine-grained analysis was conducted on four articles, two from each newspaper, selected to reflect the trends found in the corpus analysis. The fine-grained analyses showed how linguistic resources contributed to the positioning of the UK and Zimbabwe. Complementary analyses of these articles were conducted using LCT and SFL to describe how readers' knowledge of diplomatic relations is built using language in The Herald and The Standard. The study used the concepts of constellations and cosmologies from LCT to demonstrate how diplomatic knowledge is built in these news articles. In the articles, the constellations are related to policies such as Zimbabwe's reform agenda, people (diplomats and government officials) and moral judgments. Both newspapers contain both positive and negative positioning of Zimbabwe and the UK. However, The Standard is generally critical of removing the UK's sanctions on Zimbabwe and of the Zimbabwean government's reform agenda. Meanwhile, The Herald justifies Zimbabwe's Fast-track Land Reform Programme as resulting from Britain's refusal to fund a land redistribution programme as per the 1979 Lancaster House Agreement. The use of SFL's Appraisal framework helps to examine the linguistic resources used by The Herald and The Standard to describe the positioning of the two countries in relation to each other. Lexical strings help to establish diplomatic positioning in the news articles. These couple with Appraisal to accomplish individuation and affiliation. Affect and Judgement resources revealed the decisions taken by the UK to solve the conflict by encouraging the implementation of a reform agenda by Zimbabwe. Negative evaluation is consistently used to disalign with sanctions. The emerging patterns in the data show that diplomats representing the UK affiliate with Zimbabwe's stated economic and political reforms, and the government of Zimbabwe affiliates with the new trade agreement between Zimbabwe and the UK. At the same time, the UK diplomats individuate away from human rights abuses and the Fast-track Land Reform Programme. Affiliation strengthens confidence in diplomatic ties between Zimbabwe and the UK because the emphasis is placed on restoring them despite the conditions attached. The Herald and The Standard position Zimbabwe as a reformed country ready to implement policies to improve citizens' lives and as a country violating human rights and the rule of law. They position the UK as asserting power over Zimbabwe, willing to resolve the crisis through its foreign policy, and credited for assisting Zimbabwe. This strengthens re-engagement in diplomatic relations and commercial trade between the UK and Zimbabwe. In light of these findings, Zimbabweans are encouraged to cultivate an awareness that enables them to reflect on the challenges associated with diplomatic discourses and the implications for critically analysing the re-engagement initiative. They can promote re-engagement by being cognisant of specific values portrayed in The Herald and The Standard and challenging these values in the light of policy transformation to revive the relations between the two countries. The re-engagement process requires a transformation in Zimbabwe's modus operandi to improve the country's positioning in the diplomatic relations between it and the UK. , Thesis (PhD) -- Faculty of Humanities, Linguistics and Applied Language Studies, 2023
- Full Text:
- Date Issued: 2023-10-13
An exploration of Black women’s motivations for pursuing sociology at Rhodes University
- Authors: Vezi, Indiphile Siyanda
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/425220 , vital:72220
- Description: Enbargoed. Expected release date 2025. , Thesis (MA) -- Faculty of Humanities, Sociology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Vezi, Indiphile Siyanda
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/425220 , vital:72220
- Description: Enbargoed. Expected release date 2025. , Thesis (MA) -- Faculty of Humanities, Sociology, 2023
- Full Text:
- Date Issued: 2023-10-13
An interpretive phenomenological analysis of the funerary ritual crises caused by COVID-19 restrictions, and the pursuit of spirituality as a coping strategy amongst amaZulu living in the Midlands area of KwaZulu-Natal, South Africa
- Authors: Sibisi, Ntokozo Penelope
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/431498 , vital:72780
- Description: In 2020, South Africa faced a significant challenge as it grappled with approximately 500,000 COVID-19 infections, which accounted for over half of all reported cases across Africa. Living through a global pandemic such as COVID-19 had a dramatic and transformative impact on the arena of death for many South Africans, not only because of the number of deaths that have occurred globally due to COVID-19, but also how the bereaved living honour the dead. This study delves into a previously underreported facet of the pandemic's impact, focusing on the experiences of bereaved AmaZulu people who encountered profound disruptions in funerary rituals due to COVID-19 restrictions. The research aims to shed light on how spirituality may mitigate the challenges posed by these ritual constraints, drawing parallels with other ethnographies which have highlighted the importance of spirituality among the AmaZulu people of the Midlands in KwaZulu-Natal, South Africa. This study employed a mixed method approach in generating quantitative and qualitative data. Data from 101 participants who responded to an online questionnaire were analysed using STATA. Additionally, ethnographic data with 9 participants, purposively sampled from the larger sample, was analysed using an Interpretive Phenomenological Analysis (IPA) approach, which ensured that their lived experiences of bereavement took centre stage. The findings of this study illuminate the regret experienced by participants who adapted to [government] bureaucratically-imposed funerals and medical care, revealing potential implications for future pandemic responses. This research underscores the significance of customary funerary rituals, urging compassionate public health responses that acknowledge their role amid mass deaths. It also highlights the interplay between spirituality, religion, and rituals, showcasing how these elements form an intricate web in the AmaZulu community. This study contributes to the broader understanding of death and COVID-19 in South Africa, where racial disparities in COVID-19 deaths have come to the forefront. It addresses the enduring trauma within Black communities and explores how the absence of funerary rituals can strain the psychological, emotional, and spiritual fabric of the bereaved. , Thesis (MSocSci) -- Faculty of Humanities, Athropology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Sibisi, Ntokozo Penelope
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/431498 , vital:72780
- Description: In 2020, South Africa faced a significant challenge as it grappled with approximately 500,000 COVID-19 infections, which accounted for over half of all reported cases across Africa. Living through a global pandemic such as COVID-19 had a dramatic and transformative impact on the arena of death for many South Africans, not only because of the number of deaths that have occurred globally due to COVID-19, but also how the bereaved living honour the dead. This study delves into a previously underreported facet of the pandemic's impact, focusing on the experiences of bereaved AmaZulu people who encountered profound disruptions in funerary rituals due to COVID-19 restrictions. The research aims to shed light on how spirituality may mitigate the challenges posed by these ritual constraints, drawing parallels with other ethnographies which have highlighted the importance of spirituality among the AmaZulu people of the Midlands in KwaZulu-Natal, South Africa. This study employed a mixed method approach in generating quantitative and qualitative data. Data from 101 participants who responded to an online questionnaire were analysed using STATA. Additionally, ethnographic data with 9 participants, purposively sampled from the larger sample, was analysed using an Interpretive Phenomenological Analysis (IPA) approach, which ensured that their lived experiences of bereavement took centre stage. The findings of this study illuminate the regret experienced by participants who adapted to [government] bureaucratically-imposed funerals and medical care, revealing potential implications for future pandemic responses. This research underscores the significance of customary funerary rituals, urging compassionate public health responses that acknowledge their role amid mass deaths. It also highlights the interplay between spirituality, religion, and rituals, showcasing how these elements form an intricate web in the AmaZulu community. This study contributes to the broader understanding of death and COVID-19 in South Africa, where racial disparities in COVID-19 deaths have come to the forefront. It addresses the enduring trauma within Black communities and explores how the absence of funerary rituals can strain the psychological, emotional, and spiritual fabric of the bereaved. , Thesis (MSocSci) -- Faculty of Humanities, Athropology, 2023
- Full Text:
- Date Issued: 2023-10-13
Analysing equity and ethical dimensions of water governance challenges in the lower section of the upper Vaal River catchment, Gauteng, South Africa
- Authors: Tavengwa, Noleen Shamiso
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424199 , vital:72132
- Description: Poor water governance practices have been identified as one of the root causes of water problems confronting the South African water sector. This is evident in the lower section of the Upper Vaal River Catchment, where ineffective implementation of the regulatory measures has resulted in multiple incidents of illegal discharge of effluent from municipal wastewater treatment works, industrial and mining activities. Pollution and deterioration of water quality is a major challenge in the catchment; the Vaal River is severely polluted beyond acceptable standards and impacts negatively on other water users who have legitimate rights to the water resources. Pollution not only affects human activities and use, but also has negative impacts on ecological health and functionality. Poor water governance practices raise ethical implications for the rights to water for both social and ecological components. Although many water governance challenges in South Africa have implications that border on values and ethics, the relationship between these concepts has hardly been explored. In order to contribute towards filling this gap, this study explores water governance challenges and their ethical implications, and then analyse the equity dimensions of key water challenges among institutional actors in the lower section of the Upper Vaal WMA, Gauteng. The study uses a qualitative research approach which involves several methods of data collection: document analysis, workshops, focus group discussions, key informant interviews and participant observation. The data collected were analysed using thematic analysis, and then applied an ethical framework to distil ethical implications of the identified water governance challenges. There are five water governance challenges that were identified, which are i) failure in the implementation of water legislation and regulations, ii) ineffective leadership and management, iii) cooperative governance and poor accountability, iv) decentralisation and ineffective participation, and v) finance, infrastructure, and technical capacity. The deterioration of water quality and pollution due to ineffective implementation of the regulatory system has ethical implications towards protection of the ecological system and sustainable management of the water resources for the present and future generation. Ineffective implementation of the regulatory measure also raises the issue of distributive equity between different water users and between current and future users. These implications are critical particularly when viewed from the perspective of systemic-relational ethical approach which posit that in social-ecological systems, all components ought to be treated with equal regards, and their inherent worth respected in order to maintain the unity of the SES. The idea of equal regards to the inherent worth of the component implies due respect, in ways that do not undermine the integrity of the entire system. The challenges identified in the system suggest that i) the right of the ecological system to water is undermined due to anthropogenic activities, which may in the long term undermine the functionality of the entire SES; ii) the right of the margined to access to water of the right quality is also undermined, and pollution costs is externalised. These are matters of ethics that thus deserved attention in policy and management considerations. The study analysed equity dimensions (distributive and procedural), targeting the key institutional water users (i.e., mines, industries, agriculture, municipalities, and the wastewater treatment works) in the lower section of the Upper Vaal River Catchment as equity candidates due to their activities that significantly impact the environment and the water resources. The cost of pollution caused by mining activities, industrial activities and the wastewater treatment works is externalised to other users who are not responsible for the pollution. In this regard study identified key drivers that raise concerns linked to distributive equity, which are ) pollution and water quality deterioration, ii) the legacies of mining activities, and iii) poor infrastructure maintenance, vandalism, and theft. Identifying these key drivers of distributive equity concerns is critical in ensuring that the water governance processes are designed in a way that stakeholders and actors reliant on water resources within the catchment have equitable and fair access without the effects of externalised costs from other actors. The identified water governance challenges in the catchment all borders on ethics, thus an ethical framework is crucial in tackling water related problems. The principles of ethics can be used as guidelines to decision-making process for the management and governance of water resources in South Africa. Therefore, implementing and incorporating principles of the systemic-relational ethics into policy making and water resources management will yield sustainable, efficient, and equitable use and management of water resources. , Thesis (MSc) -- Faculty of Science, Institute for Water Research, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Tavengwa, Noleen Shamiso
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424199 , vital:72132
- Description: Poor water governance practices have been identified as one of the root causes of water problems confronting the South African water sector. This is evident in the lower section of the Upper Vaal River Catchment, where ineffective implementation of the regulatory measures has resulted in multiple incidents of illegal discharge of effluent from municipal wastewater treatment works, industrial and mining activities. Pollution and deterioration of water quality is a major challenge in the catchment; the Vaal River is severely polluted beyond acceptable standards and impacts negatively on other water users who have legitimate rights to the water resources. Pollution not only affects human activities and use, but also has negative impacts on ecological health and functionality. Poor water governance practices raise ethical implications for the rights to water for both social and ecological components. Although many water governance challenges in South Africa have implications that border on values and ethics, the relationship between these concepts has hardly been explored. In order to contribute towards filling this gap, this study explores water governance challenges and their ethical implications, and then analyse the equity dimensions of key water challenges among institutional actors in the lower section of the Upper Vaal WMA, Gauteng. The study uses a qualitative research approach which involves several methods of data collection: document analysis, workshops, focus group discussions, key informant interviews and participant observation. The data collected were analysed using thematic analysis, and then applied an ethical framework to distil ethical implications of the identified water governance challenges. There are five water governance challenges that were identified, which are i) failure in the implementation of water legislation and regulations, ii) ineffective leadership and management, iii) cooperative governance and poor accountability, iv) decentralisation and ineffective participation, and v) finance, infrastructure, and technical capacity. The deterioration of water quality and pollution due to ineffective implementation of the regulatory system has ethical implications towards protection of the ecological system and sustainable management of the water resources for the present and future generation. Ineffective implementation of the regulatory measure also raises the issue of distributive equity between different water users and between current and future users. These implications are critical particularly when viewed from the perspective of systemic-relational ethical approach which posit that in social-ecological systems, all components ought to be treated with equal regards, and their inherent worth respected in order to maintain the unity of the SES. The idea of equal regards to the inherent worth of the component implies due respect, in ways that do not undermine the integrity of the entire system. The challenges identified in the system suggest that i) the right of the ecological system to water is undermined due to anthropogenic activities, which may in the long term undermine the functionality of the entire SES; ii) the right of the margined to access to water of the right quality is also undermined, and pollution costs is externalised. These are matters of ethics that thus deserved attention in policy and management considerations. The study analysed equity dimensions (distributive and procedural), targeting the key institutional water users (i.e., mines, industries, agriculture, municipalities, and the wastewater treatment works) in the lower section of the Upper Vaal River Catchment as equity candidates due to their activities that significantly impact the environment and the water resources. The cost of pollution caused by mining activities, industrial activities and the wastewater treatment works is externalised to other users who are not responsible for the pollution. In this regard study identified key drivers that raise concerns linked to distributive equity, which are ) pollution and water quality deterioration, ii) the legacies of mining activities, and iii) poor infrastructure maintenance, vandalism, and theft. Identifying these key drivers of distributive equity concerns is critical in ensuring that the water governance processes are designed in a way that stakeholders and actors reliant on water resources within the catchment have equitable and fair access without the effects of externalised costs from other actors. The identified water governance challenges in the catchment all borders on ethics, thus an ethical framework is crucial in tackling water related problems. The principles of ethics can be used as guidelines to decision-making process for the management and governance of water resources in South Africa. Therefore, implementing and incorporating principles of the systemic-relational ethics into policy making and water resources management will yield sustainable, efficient, and equitable use and management of water resources. , Thesis (MSc) -- Faculty of Science, Institute for Water Research, 2023
- Full Text:
- Date Issued: 2023-10-13
Analysis of the role and regulation of HOP1a and HOP1b splice variants in cancer biology
- Authors: Schwarz, Kelly
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432240 , vital:72854
- Description: Restricted access. Expected lease date in 2025. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Schwarz, Kelly
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432240 , vital:72854
- Description: Restricted access. Expected lease date in 2025. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-10-13
Application of in vitro release testing (IVRT) to assess “sameness”/differences of topical clotrimazole formulations
- Wellington, Hannah Margaret Mary
- Authors: Wellington, Hannah Margaret Mary
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/419599 , vital:71658
- Description: Embargoed. Possible release date early 2025 pending publications. , Thesis (MSC Pharm) -- Faculty of Pharmacy, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Wellington, Hannah Margaret Mary
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/419599 , vital:71658
- Description: Embargoed. Possible release date early 2025 pending publications. , Thesis (MSC Pharm) -- Faculty of Pharmacy, 2023
- Full Text:
- Date Issued: 2023-10-13
Application of web design techniques and best practices in implementing web development, maintenance and enhancement of RUBi websites and web application systems
- Authors: Tshabalalala, Thulani
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424688 , vital:72175
- Description: The popularity of the web has seen various fields, such as the sciences taking advantage of this resource to further their scientific endeavours. This has seen science groups moving into developing websites and web applications, and such a group is the Research Unit in Bioinformative (RUBi). With the use of the web, the development and maintenance of whatever web-related tools become inevitable, given the continuous changes in the web space. This continuous evolution of web development and maintenance will come with techniques, principles and standards which will not only enable faster development of web entities but also ensure that modern hardware, fulfilment of the requirements to use such hardware and modern concepts are incorporated into forming web tools that enable such progression. Furthermore, introducing the previously mentioned progress of the web becomes an essential part of its development and maintenance. This paper did implement the processes of progressing the web using the technique of documentation and version control systems. The web development for the COVIDRUG website was done for the Covidrug-Africa Consortium (COVIDRUG) using the Django webdevelopment framework. The RUBi website and the MDM-Task we band the Job Management System (JMS) web applications were maintained for the maintenance aspect. Archives brought value regarding the traceability it provides of the various web-related aspects. The development showed a website’s potential value, particularly for research groups. The maintenance carried out showed how different techniques and approaches could be used in different maintenance prospects to achieve set objectives. The development and maintenance resulted in websites and web applications that have the features stated in their respective maintenance plans. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Tshabalalala, Thulani
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424688 , vital:72175
- Description: The popularity of the web has seen various fields, such as the sciences taking advantage of this resource to further their scientific endeavours. This has seen science groups moving into developing websites and web applications, and such a group is the Research Unit in Bioinformative (RUBi). With the use of the web, the development and maintenance of whatever web-related tools become inevitable, given the continuous changes in the web space. This continuous evolution of web development and maintenance will come with techniques, principles and standards which will not only enable faster development of web entities but also ensure that modern hardware, fulfilment of the requirements to use such hardware and modern concepts are incorporated into forming web tools that enable such progression. Furthermore, introducing the previously mentioned progress of the web becomes an essential part of its development and maintenance. This paper did implement the processes of progressing the web using the technique of documentation and version control systems. The web development for the COVIDRUG website was done for the Covidrug-Africa Consortium (COVIDRUG) using the Django webdevelopment framework. The RUBi website and the MDM-Task we band the Job Management System (JMS) web applications were maintained for the maintenance aspect. Archives brought value regarding the traceability it provides of the various web-related aspects. The development showed a website’s potential value, particularly for research groups. The maintenance carried out showed how different techniques and approaches could be used in different maintenance prospects to achieve set objectives. The development and maintenance resulted in websites and web applications that have the features stated in their respective maintenance plans. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-10-13
Arbuscular mycorrhizal fungi associated with maize in South Africa, under conventional and conservation agricultural cultivation
- Maússe Sitoe, Sílvia Natal David
- Authors: Maússe Sitoe, Sílvia Natal David
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431846 , vital:72808
- Description: Access restricted. Expected release date in 2025. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Maússe Sitoe, Sílvia Natal David
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431846 , vital:72808
- Description: Access restricted. Expected release date in 2025. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-10-13
Arbuscular mycorrhizal fungi associated with wheat under conventional and conservation agricultural cultivation
- Authors: Dube, Makasithembe
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431688 , vital:72796
- Description: Access restricted. Expected release date in 2024. , Thesis (PhD) -- Faculty of Science, Chemistry, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Dube, Makasithembe
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431688 , vital:72796
- Description: Access restricted. Expected release date in 2024. , Thesis (PhD) -- Faculty of Science, Chemistry, 2023
- Full Text:
- Date Issued: 2023-10-13
Asymmetrical zinc(II) phthalocyanines conjugated to nanomaterials for degradation of organic pollutants and inactivation of Staphylococcus aureus bacteria
- Authors: Mgidlana, Sithi
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431859 , vital:72809 , DOI 10.21504/10962/431860
- Description: This thesis reports on the syntheses and characterization of asymmetrical phthalocyanines (Pcs) with different ring substituents (tert-butyl, ester, diimide, trimethoxy, acetophenone, heptanoylphenoxy, perfluorophenoxy, dimethoxy, propanoic acid, acetic acid, carboxylic acid, aminophenoxy, acrylic acid). Several nanoparticles including metal tungstate, capped with glutathione and 1-mercaptohexanol are prepared and characterized using analytical techniques. The synthesized Pcs are covalently linked to various nanoparticles (NPs) through ester and amide bonds to form Pc-NP conjugates, in order to improve their catalytic properties. The Pcs and their conjugates are characterized using different analytical techniques. The photophysics and photochemistry of the MPcs and conjugates are studied. The conjugates T). The complexes and the conjugates with nanomaterials are evaluated for singlet oxygen-generating ability. Conjugates generate higher singlet oxygen in comparison to Pc complexes alone. The photocatalytic activity of the conjugates of ZnPc complexes with NiWO4, Ag2WO4, Bi2WO6, CoWO4, and Ag-Fe3O4-based nanoparticles is evaluated based on photodegradation of methylene blue, tetracycline, and dibenzothiophene. The photocatalytic efficiencies of the synthesized phthalocyanine complexes increased in the presence of nanoparticles. This work also reports on the photodynamic antimicrobial chemotherapy activity of these materials against Staphylococcus aureus (S. aureus) bacteria in DMSO. The results indicated that silver-based nanoconjugates exhibit high antimicrobial activity with high log reductions compared to NiWO4, CoWO4, and Ag-Fe3O4-based materials. The z-scan technique is employed to experimentally test the nonlinear optical response of complexes and nanoconjugates in solution. The nonlinear absorption coefficient, third-order optical susceptibility and optical limiting threshold of the materials are obtained from the Z-scan aperture data. The nonlinear absorption parameters improved in the presence of semiconductor quantum dots, with 1-ethanoic-CdTe/ZnSeS/ZnO giving the best results due to the presence of electron-donating substituents. , Thesis (PhD) -- Faculty of Science, Chemistry, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Mgidlana, Sithi
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431859 , vital:72809 , DOI 10.21504/10962/431860
- Description: This thesis reports on the syntheses and characterization of asymmetrical phthalocyanines (Pcs) with different ring substituents (tert-butyl, ester, diimide, trimethoxy, acetophenone, heptanoylphenoxy, perfluorophenoxy, dimethoxy, propanoic acid, acetic acid, carboxylic acid, aminophenoxy, acrylic acid). Several nanoparticles including metal tungstate, capped with glutathione and 1-mercaptohexanol are prepared and characterized using analytical techniques. The synthesized Pcs are covalently linked to various nanoparticles (NPs) through ester and amide bonds to form Pc-NP conjugates, in order to improve their catalytic properties. The Pcs and their conjugates are characterized using different analytical techniques. The photophysics and photochemistry of the MPcs and conjugates are studied. The conjugates T). The complexes and the conjugates with nanomaterials are evaluated for singlet oxygen-generating ability. Conjugates generate higher singlet oxygen in comparison to Pc complexes alone. The photocatalytic activity of the conjugates of ZnPc complexes with NiWO4, Ag2WO4, Bi2WO6, CoWO4, and Ag-Fe3O4-based nanoparticles is evaluated based on photodegradation of methylene blue, tetracycline, and dibenzothiophene. The photocatalytic efficiencies of the synthesized phthalocyanine complexes increased in the presence of nanoparticles. This work also reports on the photodynamic antimicrobial chemotherapy activity of these materials against Staphylococcus aureus (S. aureus) bacteria in DMSO. The results indicated that silver-based nanoconjugates exhibit high antimicrobial activity with high log reductions compared to NiWO4, CoWO4, and Ag-Fe3O4-based materials. The z-scan technique is employed to experimentally test the nonlinear optical response of complexes and nanoconjugates in solution. The nonlinear absorption coefficient, third-order optical susceptibility and optical limiting threshold of the materials are obtained from the Z-scan aperture data. The nonlinear absorption parameters improved in the presence of semiconductor quantum dots, with 1-ethanoic-CdTe/ZnSeS/ZnO giving the best results due to the presence of electron-donating substituents. , Thesis (PhD) -- Faculty of Science, Chemistry, 2023
- Full Text:
- Date Issued: 2023-10-13
Beyond mastery: jazz, gender and power in postapartheid South Africa
- Authors: Williams, Ulagh
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432505 , vital:72876 , DOI 10.21504/10962/432512
- Description: This dissertation studies the musical lives of seven South African women who have built highprofile national and international careers as jazz musicians. Empirically it records self-reported experiences and actions that they have identified as relevant to their success within a notoriously patriarchal field. Drawing on feminist research methods and Interpretative Phenomenological Analysis the dissertation strives to develop an empathic yet critical epistemological framework attuned to the complexities of their lived experiences as women and as jazz musicians. Theoretically it investigates the power relations at play in the myriad ways they negotiate or have been impelled to negotiate gender and patriarchy as musicians. Chapter one draws attention to the ubiquity of patriarchy in South African and international jazz culture, and highlights the research participants’ numerous successes to date as performers, educators, composers, and bandleaders. Chapter two positions this study in relation to cognate work in South African jazz studies, international feminist jazz studies and feminist phenomenology. Chapters three to five successively consider the participants’ early enculturation as musical and gendered subjects, their agentic responses to structures of patriarchy and/or race as emerging and established professionals, and the ways these experiences have found expression in some of their musical utterances as performers and composers. Fusing art and activism, the participants challenge and transcend masculinist discourses of mastery that still dominate South African jazz as a field of production and aesthetic practice. , Thesis (PhD) -- Faculty of Humanities, Music and Musicology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Williams, Ulagh
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432505 , vital:72876 , DOI 10.21504/10962/432512
- Description: This dissertation studies the musical lives of seven South African women who have built highprofile national and international careers as jazz musicians. Empirically it records self-reported experiences and actions that they have identified as relevant to their success within a notoriously patriarchal field. Drawing on feminist research methods and Interpretative Phenomenological Analysis the dissertation strives to develop an empathic yet critical epistemological framework attuned to the complexities of their lived experiences as women and as jazz musicians. Theoretically it investigates the power relations at play in the myriad ways they negotiate or have been impelled to negotiate gender and patriarchy as musicians. Chapter one draws attention to the ubiquity of patriarchy in South African and international jazz culture, and highlights the research participants’ numerous successes to date as performers, educators, composers, and bandleaders. Chapter two positions this study in relation to cognate work in South African jazz studies, international feminist jazz studies and feminist phenomenology. Chapters three to five successively consider the participants’ early enculturation as musical and gendered subjects, their agentic responses to structures of patriarchy and/or race as emerging and established professionals, and the ways these experiences have found expression in some of their musical utterances as performers and composers. Fusing art and activism, the participants challenge and transcend masculinist discourses of mastery that still dominate South African jazz as a field of production and aesthetic practice. , Thesis (PhD) -- Faculty of Humanities, Music and Musicology, 2023
- Full Text:
- Date Issued: 2023-10-13
Characterizing shift systems and exploring their effects on sleep and fatigue in South African freight railway operators
- Authors: Mona, Chumani
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424300 , vital:72141
- Description: Embargoed. Expected release date 2025. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Mona, Chumani
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424300 , vital:72141
- Description: Embargoed. Expected release date 2025. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2023
- Full Text:
- Date Issued: 2023-10-13
COVID19 and accountability in South Africa: legislation, ethics and disaster risk management
- Authors: Chapman, Emma Deidre
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/419565 , vital:71655
- Description: Embargoed. Possible release in 2026 pending publication. , Thesis (MSC Pharm) -- Faculty of Pharmacy, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Chapman, Emma Deidre
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/419565 , vital:71655
- Description: Embargoed. Possible release in 2026 pending publication. , Thesis (MSC Pharm) -- Faculty of Pharmacy, 2023
- Full Text:
- Date Issued: 2023-10-13
Design of an LMS-mediated tutorial to support deep and effective engagement in the process of learning mathematics
- Authors: Kigundu, Stephen
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431565 , vital:72786 , DOI 10.21504/10962/431565
- Description: Many institutions of Higher Learning have adopted a variety of Learning Management Systems (LMS) as platforms for e-learning implementation. However, the design and nature of LMS technologies present challenges to the design of LMS-based activities that engage students in learning to do mathematics. There are no clear guidelines or strategies for designing LMS-based activities that engage students in complex mathematical processes. Hence, using technologies in mathematics education often replicates instructivist positions and practices. Conversely, using constructivist principles, modes of mathematical engagement, and e-learning tools to mediate learning provides an integrated framework to transform the use of an LMS as a platform for e-learning implementation and promote deep engagement in mathematical learning. The objective of this study was to explore mechanisms that could be useful for conceiving activities to support the learning of Mathematics using an LMS-mediated tutorial. The design, redesign and evaluation of the e-tutorial are reported upon. In preparation for the study, an LMS-based tutorial was designed and developed to be used as a test-bed to investigate how these e-learning tools could support learning to do mathematics. A Trigonometry module, consisting of course content in the form of resources and tasks to help the students to explore, practice and apply right triangle concepts, was used to investigate and derive design strategies. A mixed method research approach with a reflexive self-study research design was used. A group of first-year university student volunteers studying mathematics in the mechanical engineering department were used to test the tutorial. The students were asked to do a series of tasks using the e-learning environment during the Mathematics tutorial period. Data was collected using in-class observations, interviews, screen capture videos, student-written responses, and system-generated data. All students were encouraged to complete a learning journal detailing their experiences during the tutorial using an LMS-based tool. The students were given no training, but a tutor (researcher) was available to answer any questions they may have had. Contradiction analysis was used to evaluate the data, compare purpose and practice and judge whether the activity or tool was fitted for the intended purpose. Findings were in the form of transformations of the e-tutorial system as it was developed. Among the most noteworthy contributory modifications were changing from 1) “read first, then do” to “do first, read when necessary”, 2) “work on the computer” to “work on paper then capture on the computer”, 3) “physically separated work with computer-enabled social contact”, to "individual computer work in face-to-face social settings.” 4) “single-level of resource provision” to “multi-level, demand-driven resource provision” and 5) “self-regulated” learning process regulation to “computer-assisted” learning process regulation. The discussion of these findings indicated that to enable students’ deep and effective engagement in the process of learning fundamental trigonometry within an LMS-mediated tutorial, one needs to design the system with some activities that can create a demand for knowledge, encourage rough work and face-to-face social interaction, supported by multi-level, demand-driven resources, and computer-assisted learning-process regulation. Contributions to research by this study were in the form of 1) Design Principles for LMS-mediated tutorials, 2) Principles for an e-tutorial development methodology, and 3) The LMS-mediated tutorial system. , Thesis (PhD) -- Faculty of Education, Primary and Early Childhood Education, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Kigundu, Stephen
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431565 , vital:72786 , DOI 10.21504/10962/431565
- Description: Many institutions of Higher Learning have adopted a variety of Learning Management Systems (LMS) as platforms for e-learning implementation. However, the design and nature of LMS technologies present challenges to the design of LMS-based activities that engage students in learning to do mathematics. There are no clear guidelines or strategies for designing LMS-based activities that engage students in complex mathematical processes. Hence, using technologies in mathematics education often replicates instructivist positions and practices. Conversely, using constructivist principles, modes of mathematical engagement, and e-learning tools to mediate learning provides an integrated framework to transform the use of an LMS as a platform for e-learning implementation and promote deep engagement in mathematical learning. The objective of this study was to explore mechanisms that could be useful for conceiving activities to support the learning of Mathematics using an LMS-mediated tutorial. The design, redesign and evaluation of the e-tutorial are reported upon. In preparation for the study, an LMS-based tutorial was designed and developed to be used as a test-bed to investigate how these e-learning tools could support learning to do mathematics. A Trigonometry module, consisting of course content in the form of resources and tasks to help the students to explore, practice and apply right triangle concepts, was used to investigate and derive design strategies. A mixed method research approach with a reflexive self-study research design was used. A group of first-year university student volunteers studying mathematics in the mechanical engineering department were used to test the tutorial. The students were asked to do a series of tasks using the e-learning environment during the Mathematics tutorial period. Data was collected using in-class observations, interviews, screen capture videos, student-written responses, and system-generated data. All students were encouraged to complete a learning journal detailing their experiences during the tutorial using an LMS-based tool. The students were given no training, but a tutor (researcher) was available to answer any questions they may have had. Contradiction analysis was used to evaluate the data, compare purpose and practice and judge whether the activity or tool was fitted for the intended purpose. Findings were in the form of transformations of the e-tutorial system as it was developed. Among the most noteworthy contributory modifications were changing from 1) “read first, then do” to “do first, read when necessary”, 2) “work on the computer” to “work on paper then capture on the computer”, 3) “physically separated work with computer-enabled social contact”, to "individual computer work in face-to-face social settings.” 4) “single-level of resource provision” to “multi-level, demand-driven resource provision” and 5) “self-regulated” learning process regulation to “computer-assisted” learning process regulation. The discussion of these findings indicated that to enable students’ deep and effective engagement in the process of learning fundamental trigonometry within an LMS-mediated tutorial, one needs to design the system with some activities that can create a demand for knowledge, encourage rough work and face-to-face social interaction, supported by multi-level, demand-driven resources, and computer-assisted learning-process regulation. Contributions to research by this study were in the form of 1) Design Principles for LMS-mediated tutorials, 2) Principles for an e-tutorial development methodology, and 3) The LMS-mediated tutorial system. , Thesis (PhD) -- Faculty of Education, Primary and Early Childhood Education, 2023
- Full Text:
- Date Issued: 2023-10-13