- Title
- Improving the bioremediation of phenolic wastewaters by Trametes versicolor
- Creator
- Ryan, D, Leukes, Winston D, Burton, Stephanie G
- Date
- 2006
- Type
- Article
- Identifier
- vital:6467
- Identifier
- http://hdl.handle.net/10962/d1005796
- Identifier
- http://dx.doi.org/10.1016/j.biortech.2006.02.001
- Description
- The successful bioremediation of a phenolic wastewater by Trametes versicolor was found to be dependent on a range of factors including: fungal growth, culture age and activity and enzyme (laccase) production. These aspects were enhanced by the optimisation of the growth medium used and time of addition of the pollutant to the fungal cultures. Different media containing ‘high’ (20 g/L), ‘low’ (2 g/L) and ‘sufficient’ (10 g/L) concentrations of carbon and nitrogen sources were investigated. The medium containing both glucose and peptone at 10 g/L resulted in the highest Growth Related Productivity (the product of specific yield and μ) of laccase (1.46 Units of laccase activity)/gram biomass/day and was used in all further experiments. The use of the guaiacol as an inducer further increased laccase activity 780% without inhibiting growth; similarly the phenolic effluent studied boosted activity almost 5 times. The timing of the addition of the phenolic effluent was found to have important consequences in its removal and at least 8 days of prior growth was required. Under these conditions, 0.125 g phenol/g biomass and 0.231 g o-cresol/g biomass were removed from solution per day.
- Format
- 12 pages, pdf
- Language
- English
- Relation
- Ryan, D. and Leukes, W. and Burton, S. (2006) Improving the bioremediation of phenolic wastewaters by Trametes versicolor. Bioresource Technology, 98 (3). pp. 579-587. Available: dx.doi.org/10.1016/j.biortech.2006.02.001
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Burton, Stephanie Gail (Prof)
Leukes, Winston Daniel (Dr)
SDG 06. Clean Water and Sanitation
RU Department of Biochemistry, Microbiology and Bioinformatics
SDG 03. Good Health and Well-Being
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| View Details | SOURCEPDF | 490 KB | Adobe Acrobat PDF | View Details |