High performance liquid chromatographic analysis of oleandomycin in serum and urine
- Stubbs, Christopher, Haigh, John M, Kanfer, Isadore
- Authors: Stubbs, Christopher , Haigh, John M , Kanfer, Isadore
- Date: 1986
- Language: English
- Type: text , Article
- Identifier: vital:6429 , http://hdl.handle.net/10962/d1006590
- Description: The determination of oleandomycin in serum and urine by high-performance liquid chromatography using erythromycin as internal standard is described. The separation was achieved on a reversed-phase C 1 s column employing acetonitrile-0.05 A4 phosphate buffer (30:70), adjusted to pH 7.0, as the mobile phase with UV detection at 200 nm. A solid-phase extraction procedure, combined with a simple phaseseparation step was used prior to chromatographic analysis. Linear calibration curves were obtained in the concentration ranges 0.25-5.0 pg/ml (serum) and 1 .O-25.0 pg/ml (urine). Precise quantitative analysis has been achieved at these levels with relative standard deviations of < 5%.
- Full Text:
- Authors: Stubbs, Christopher , Haigh, John M , Kanfer, Isadore
- Date: 1986
- Language: English
- Type: text , Article
- Identifier: vital:6429 , http://hdl.handle.net/10962/d1006590
- Description: The determination of oleandomycin in serum and urine by high-performance liquid chromatography using erythromycin as internal standard is described. The separation was achieved on a reversed-phase C 1 s column employing acetonitrile-0.05 A4 phosphate buffer (30:70), adjusted to pH 7.0, as the mobile phase with UV detection at 200 nm. A solid-phase extraction procedure, combined with a simple phaseseparation step was used prior to chromatographic analysis. Linear calibration curves were obtained in the concentration ranges 0.25-5.0 pg/ml (serum) and 1 .O-25.0 pg/ml (urine). Precise quantitative analysis has been achieved at these levels with relative standard deviations of < 5%.
- Full Text:
Reply to correspondence: P.M. Gaylarde (1986) The human skin blanching assay—use and abuse
- Haigh, John M, Kanfer, Isadore, Meyer, Eric, Smith, Eric W
- Authors: Haigh, John M , Kanfer, Isadore , Meyer, Eric , Smith, Eric W
- Date: 1986
- Language: English
- Type: Article
- Identifier: vital:6376 , http://hdl.handle.net/10962/d1006293
- Description: Finally, we would like to assure Dr Gaylarde that we do not advocate the use of the human skin blanching assay. There are several other in vivo methods for determining corticosteroid activity which will provide equally meaningful results. What we are advocating is that if the human skin blanching assay is going to be used, then it should be used properly.
- Full Text:
- Authors: Haigh, John M , Kanfer, Isadore , Meyer, Eric , Smith, Eric W
- Date: 1986
- Language: English
- Type: Article
- Identifier: vital:6376 , http://hdl.handle.net/10962/d1006293
- Description: Finally, we would like to assure Dr Gaylarde that we do not advocate the use of the human skin blanching assay. There are several other in vivo methods for determining corticosteroid activity which will provide equally meaningful results. What we are advocating is that if the human skin blanching assay is going to be used, then it should be used properly.
- Full Text:
A stability-indicating HPLC assay with on-line clean-up for betamethasone 17-valerate in topical dosage forms
- Smith, Eric W, Haigh, John M, Kanfer, Isadore
- Authors: Smith, Eric W , Haigh, John M , Kanfer, Isadore
- Date: 1985
- Language: English
- Type: Article
- Identifier: vital:6421 , http://hdl.handle.net/10962/d1006556
- Description: A stability-indicating high-performance liquid chromatographic method with on-line clean-up has been developed for the analysis of betamethasone 17-valerate in topical dosage forms. A short pre-column containing 10 μm octadecylsilane mounted into the sample loop position of an injection valve was used as the primary clean-up step. The utilization of a diode-array UV detector allowed the quantitative analysis of betamethasone 17-valerate together with its degradation product, betamethasone 21-valerate, as well as the qualitative analysis of these compounds, relevant internal standards and the preservatives chlorocresol and methyl hydroxybenzoate contained in the cream and lotion formulations, respectively. Typically, cream and lotion dosage forms were dissolved in acetonitrile and ointments in tetrahydrofuran, internal standards added and aliquots injected onto the analytical system. Dosage form excipients were retained on the loop column and back-flushed to waste with the aid of a second solvent pump while components of interest were allowed to transfer to the analytical column for quantitative analysis. The method is accurate, precise and stability indicating and permits the rapid on-line analysis of betamethasone 17-valerate from complex topical formulation matrices without prior extractions.
- Full Text:
- Authors: Smith, Eric W , Haigh, John M , Kanfer, Isadore
- Date: 1985
- Language: English
- Type: Article
- Identifier: vital:6421 , http://hdl.handle.net/10962/d1006556
- Description: A stability-indicating high-performance liquid chromatographic method with on-line clean-up has been developed for the analysis of betamethasone 17-valerate in topical dosage forms. A short pre-column containing 10 μm octadecylsilane mounted into the sample loop position of an injection valve was used as the primary clean-up step. The utilization of a diode-array UV detector allowed the quantitative analysis of betamethasone 17-valerate together with its degradation product, betamethasone 21-valerate, as well as the qualitative analysis of these compounds, relevant internal standards and the preservatives chlorocresol and methyl hydroxybenzoate contained in the cream and lotion formulations, respectively. Typically, cream and lotion dosage forms were dissolved in acetonitrile and ointments in tetrahydrofuran, internal standards added and aliquots injected onto the analytical system. Dosage form excipients were retained on the loop column and back-flushed to waste with the aid of a second solvent pump while components of interest were allowed to transfer to the analytical column for quantitative analysis. The method is accurate, precise and stability indicating and permits the rapid on-line analysis of betamethasone 17-valerate from complex topical formulation matrices without prior extractions.
- Full Text:
Determination of erythromycin in serum and urine by high performance liquid chromatography with ultraviolet detection
- Stubbs, Christopher, Haigh, John M, Kanfer, Isadore
- Authors: Stubbs, Christopher , Haigh, John M , Kanfer, Isadore
- Date: 1985
- Language: English
- Type: text , Article
- Identifier: vital:6428 , http://hdl.handle.net/10962/d1006576
- Description: A high-performance liquid chromatographic analysis of erythromycin in human serum and urine with UV detection at 200 nm is presented. The method involves a solid-phase extraction procedure followed by a simple phase separation step and chromatography on a reversed-phase column. The method has sensitivity limits of 0.25 and 1.0 g/mL in serum and urine, respectively, and is sufficiently sensitive to monitor concentrations of erythromycin in human serum and urine after the administration of a single 500-mg erythromycin stearate tablet.
- Full Text:
- Authors: Stubbs, Christopher , Haigh, John M , Kanfer, Isadore
- Date: 1985
- Language: English
- Type: text , Article
- Identifier: vital:6428 , http://hdl.handle.net/10962/d1006576
- Description: A high-performance liquid chromatographic analysis of erythromycin in human serum and urine with UV detection at 200 nm is presented. The method involves a solid-phase extraction procedure followed by a simple phase separation step and chromatography on a reversed-phase column. The method has sensitivity limits of 0.25 and 1.0 g/mL in serum and urine, respectively, and is sufficiently sensitive to monitor concentrations of erythromycin in human serum and urine after the administration of a single 500-mg erythromycin stearate tablet.
- Full Text:
Relative potencies of topical corticosteroid formulations
- Haigh, John M, Kanfer, Isadore, Meyer, Eric, Smith, Eric W
- Authors: Haigh, John M , Kanfer, Isadore , Meyer, Eric , Smith, Eric W
- Date: 1985
- Language: English
- Type: Article
- Identifier: vital:6375 , http://hdl.handle.net/10962/d1006291
- Description: It seems to us, and others (Burdick, 1974), that multiple reading times are essential to produce the response-time profile. Comparisons of potencies of topical corticosteroid formulations should only be made on the basis of area under the curve measurements and statistical treatment of all values obtained at each reading time throughout the course of the experiment.
- Full Text:
- Authors: Haigh, John M , Kanfer, Isadore , Meyer, Eric , Smith, Eric W
- Date: 1985
- Language: English
- Type: Article
- Identifier: vital:6375 , http://hdl.handle.net/10962/d1006291
- Description: It seems to us, and others (Burdick, 1974), that multiple reading times are essential to produce the response-time profile. Comparisons of potencies of topical corticosteroid formulations should only be made on the basis of area under the curve measurements and statistical treatment of all values obtained at each reading time throughout the course of the experiment.
- Full Text:
Assessment of topical corticosteroid preparations: the human skin-blanching assay
- Haigh, John M, Kanfer, Isadore
- Authors: Haigh, John M , Kanfer, Isadore
- Date: 1984
- Language: English
- Type: text , Article
- Identifier: vital:6374 , http://hdl.handle.net/10962/d1006284
- Description: (From the introduction) Since the introduction of topical corticosteroid formulations, their use has become widespread, being prescribed for a large variety of dermatological conditions. This widespread use has created a need for a reliable method of assessing the various dosage forms of these compounds. Clinical trials are laborious, costly and difficult to mount as well as being impractical for the screening of large numbers of drugs. Patients suffering from dermatological complaints are not ideal subjects for the testing of topical corticosteroid preparations as it is difficult to obtain standardized lesions which are necessary for the comparison of results between patients (Baker and Sattar, 1968). For these reasons a number of methods have been developed for the screening of novel corticosteroids and testing of topical corticosteroid formulations.
- Full Text:
- Authors: Haigh, John M , Kanfer, Isadore
- Date: 1984
- Language: English
- Type: text , Article
- Identifier: vital:6374 , http://hdl.handle.net/10962/d1006284
- Description: (From the introduction) Since the introduction of topical corticosteroid formulations, their use has become widespread, being prescribed for a large variety of dermatological conditions. This widespread use has created a need for a reliable method of assessing the various dosage forms of these compounds. Clinical trials are laborious, costly and difficult to mount as well as being impractical for the screening of large numbers of drugs. Patients suffering from dermatological complaints are not ideal subjects for the testing of topical corticosteroid preparations as it is difficult to obtain standardized lesions which are necessary for the comparison of results between patients (Baker and Sattar, 1968). For these reasons a number of methods have been developed for the screening of novel corticosteroids and testing of topical corticosteroid formulations.
- Full Text:
Comparative bioavailability of some locally manufactured betamethasone valerate containing preparations
- Meyer, Eric, Haigh, John M, Kanfer, Isadore
- Authors: Meyer, Eric , Haigh, John M , Kanfer, Isadore
- Date: 1983
- Language: English
- Type: Article
- Identifier: vital:6399 , http://hdl.handle.net/10962/d1006326
- Description: The bioavailabilities of three locally manufactured proprietary betamethasone- 17-valerate containing creams and ointments were compared by measuring their abilities to cause blanching of human skin after topical application. The preparations studied were Betnovate Cream and Ointment, Celestoderm-V Cream and Ointment and Persivate Cream and Ointment. Celestoderm-V cream displayed a significantly superior blanching activity over both Betnovate and Persivate creams in' the occluded mode, whereas Persivate cream displayed a significantly superior blanching activity over both Betnovate and Celestoderm-V creams in the unoccluded mode. Persivate ointment was found to produce a significantly superior blanching activity over Betnovate and Celestoderm-V ointments in both the occluded and unoccluded modes of application.
- Full Text:
- Authors: Meyer, Eric , Haigh, John M , Kanfer, Isadore
- Date: 1983
- Language: English
- Type: Article
- Identifier: vital:6399 , http://hdl.handle.net/10962/d1006326
- Description: The bioavailabilities of three locally manufactured proprietary betamethasone- 17-valerate containing creams and ointments were compared by measuring their abilities to cause blanching of human skin after topical application. The preparations studied were Betnovate Cream and Ointment, Celestoderm-V Cream and Ointment and Persivate Cream and Ointment. Celestoderm-V cream displayed a significantly superior blanching activity over both Betnovate and Persivate creams in' the occluded mode, whereas Persivate cream displayed a significantly superior blanching activity over both Betnovate and Celestoderm-V creams in the unoccluded mode. Persivate ointment was found to produce a significantly superior blanching activity over Betnovate and Celestoderm-V ointments in both the occluded and unoccluded modes of application.
- Full Text:
Determination of phenylpropanolamine in serum and urine by high performance liquid chromatography
- Dowse, Roslind, Haigh, John M, Kanfer, Isadore
- Authors: Dowse, Roslind , Haigh, John M , Kanfer, Isadore
- Date: 1983
- Language: English
- Type: Article
- Identifier: vital:6361 , http://hdl.handle.net/10962/d1006056
- Description: A high-performance liquid chromatographic analysis of phenylpropanolamine in human serum and urine without prior derivatization is presented. Using direct UV detection the method is sufficiently sensitive to detect 25 ng of drug/ml of serum or urine; the coefficients of variation at 25 ng/ml and 500 ng/ml were 5.16 and 2.12, respectively, in serum. The method involves serum and urine extraction at a basic pH with chloroform, a single back-extraction, and chromatography on a reverse-phase column. Serum and urine data following administration of a single 150-mg sustained-release tablet of phenylpropanolamine hydrochloride in 6 healthy volunteers demonstrates the suitability of the analytical method.
- Full Text:
- Authors: Dowse, Roslind , Haigh, John M , Kanfer, Isadore
- Date: 1983
- Language: English
- Type: Article
- Identifier: vital:6361 , http://hdl.handle.net/10962/d1006056
- Description: A high-performance liquid chromatographic analysis of phenylpropanolamine in human serum and urine without prior derivatization is presented. Using direct UV detection the method is sufficiently sensitive to detect 25 ng of drug/ml of serum or urine; the coefficients of variation at 25 ng/ml and 500 ng/ml were 5.16 and 2.12, respectively, in serum. The method involves serum and urine extraction at a basic pH with chloroform, a single back-extraction, and chromatography on a reverse-phase column. Serum and urine data following administration of a single 150-mg sustained-release tablet of phenylpropanolamine hydrochloride in 6 healthy volunteers demonstrates the suitability of the analytical method.
- Full Text:
Phenylpropanolamine hydrochloride
- Kanfer, Isadore, Haigh, John M, Dowse, Roslind
- Authors: Kanfer, Isadore , Haigh, John M , Dowse, Roslind
- Date: 1983
- Language: English
- Type: Article
- Identifier: vital:6385 , http://hdl.handle.net/10962/d1006306
- Description: Phenylpropanolamine hydrochloride belongs to the sympathomimetic amine class of drugs and is structurally related to ephedrine hydrochloride. Its synthesis was first reported in 1910 and the first American patent was registered in 1939. The effects of phenylpropanolamine hydrochloride are largely the result of alpha-adrenergic agonist activity resulting from both direct stimulation of adrenergic receptors and release of neuronal norepinephrine. The principal adverse effect of phenylpropanolamine hydrochloride is dose-related hypertension and ventricular arrhythmia has been described. Phenylpropanolamine hydrochloride is widely used as a decongestant and it has been used as an anorectic agent for over 40 years. A report in 1939 described its effect as an hypertensive agent when administered parenterally.
- Full Text:
- Authors: Kanfer, Isadore , Haigh, John M , Dowse, Roslind
- Date: 1983
- Language: English
- Type: Article
- Identifier: vital:6385 , http://hdl.handle.net/10962/d1006306
- Description: Phenylpropanolamine hydrochloride belongs to the sympathomimetic amine class of drugs and is structurally related to ephedrine hydrochloride. Its synthesis was first reported in 1910 and the first American patent was registered in 1939. The effects of phenylpropanolamine hydrochloride are largely the result of alpha-adrenergic agonist activity resulting from both direct stimulation of adrenergic receptors and release of neuronal norepinephrine. The principal adverse effect of phenylpropanolamine hydrochloride is dose-related hypertension and ventricular arrhythmia has been described. Phenylpropanolamine hydrochloride is widely used as a decongestant and it has been used as an anorectic agent for over 40 years. A report in 1939 described its effect as an hypertensive agent when administered parenterally.
- Full Text:
Possible dosage regimens for topical steroids assessed by vasoconstrictor assays using multiple applications
- Woodford, R, Haigh, John M, Barry, B W
- Authors: Woodford, R , Haigh, John M , Barry, B W
- Date: 1983
- Language: English
- Type: Article
- Identifier: vital:6450 , http://hdl.handle.net/10962/d1006637
- Description: The bioavailabilities and activities of three amcinonide preparations and Betnovate cream were assessed using three multiple-dosage regimen vasoconstrictor assays in 10 volunteers. Applications were made once daily, twice daily and every alternate day with an initial three times daily loading dose applied on the first day only. Blanching responses first increased and then decreased due to tachyphylaxis. It is proposed that clinically the most advantageous dosage regimen is a once daily application with no loading dose.
- Full Text:
- Authors: Woodford, R , Haigh, John M , Barry, B W
- Date: 1983
- Language: English
- Type: Article
- Identifier: vital:6450 , http://hdl.handle.net/10962/d1006637
- Description: The bioavailabilities and activities of three amcinonide preparations and Betnovate cream were assessed using three multiple-dosage regimen vasoconstrictor assays in 10 volunteers. Applications were made once daily, twice daily and every alternate day with an initial three times daily loading dose applied on the first day only. Blanching responses first increased and then decreased due to tachyphylaxis. It is proposed that clinically the most advantageous dosage regimen is a once daily application with no loading dose.
- Full Text:
In vitro-in vivo evaluation of a sustained release phenylpropanolamine oral dosage form
- Dowse, Roslind, Haigh, John M, Kanfer, Isadore
- Authors: Dowse, Roslind , Haigh, John M , Kanfer, Isadore
- Date: 1982
- Language: English
- Type: Article
- Identifier: vital:6360 , http://hdl.handle.net/10962/d1006052
- Description: There is increasing interest in measuring pharmacokinetic parameters of phenylpropanolamine (PPA), a sympathomimetic amine used in over-the-counter nasal decongestants and anorectic formulations. A high pressure liquid chromatographic (HPLC) procedure was developed to enable direct ultraviolet detection of PPA, after extraction from serum and urine, without prior derivatization of the drug. This method was used to assay samples obtained from a bioavailability study of BUBtained-releasePPA tablets. The mean serum and urine profiles obtained are presented. The sustained-release tablets were subjected to dissolution testing utilizing the United States Pharmacopoeia (USP XIX) rotating basket method. An internal standard was incorporated into the dissolution fluid to enable direct analysis of the samples by HPLC. A comparison of three different dissolution fluid regimens was carried out to determine if release of the drug was affected by the change in pH of the medium and to select the most convenient method for the final dissolution studies. Some preliminary observations relating to correlations between rate of drug release from the sustained-release dosage form and percent drug absorbed are presented.
- Full Text:
- Authors: Dowse, Roslind , Haigh, John M , Kanfer, Isadore
- Date: 1982
- Language: English
- Type: Article
- Identifier: vital:6360 , http://hdl.handle.net/10962/d1006052
- Description: There is increasing interest in measuring pharmacokinetic parameters of phenylpropanolamine (PPA), a sympathomimetic amine used in over-the-counter nasal decongestants and anorectic formulations. A high pressure liquid chromatographic (HPLC) procedure was developed to enable direct ultraviolet detection of PPA, after extraction from serum and urine, without prior derivatization of the drug. This method was used to assay samples obtained from a bioavailability study of BUBtained-releasePPA tablets. The mean serum and urine profiles obtained are presented. The sustained-release tablets were subjected to dissolution testing utilizing the United States Pharmacopoeia (USP XIX) rotating basket method. An internal standard was incorporated into the dissolution fluid to enable direct analysis of the samples by HPLC. A comparison of three different dissolution fluid regimens was carried out to determine if release of the drug was affected by the change in pH of the medium and to select the most convenient method for the final dissolution studies. Some preliminary observations relating to correlations between rate of drug release from the sustained-release dosage form and percent drug absorbed are presented.
- Full Text:
Comparative blanching activities of some topical corticosteroid containing lotions
- Meyer, I, Kanfer, Isadore, Haigh, John M
- Authors: Meyer, I , Kanfer, Isadore , Haigh, John M
- Date: 1981
- Language: English
- Type: Article
- Identifier: vital:6398 , http://hdl.handle.net/10962/d1006324
- Description: The blanching activities of Betnovate and Celestoderm-V lotions (betamethasone-17-valerate, 0,1%) and Diprosone lotion (betamethasone dipropionate, 0,55%) were determined by measuring their ability to cause blanching of human skin after topical application. Betnovate and Celestoderm-V lotions produced almost identical blanching profiles. Diprosone lotion displayed a statistically significant superior blanching acitivity over both Betnovate and Celestoderm-V lotions over the whole timespan of the trial.
- Full Text:
- Authors: Meyer, I , Kanfer, Isadore , Haigh, John M
- Date: 1981
- Language: English
- Type: Article
- Identifier: vital:6398 , http://hdl.handle.net/10962/d1006324
- Description: The blanching activities of Betnovate and Celestoderm-V lotions (betamethasone-17-valerate, 0,1%) and Diprosone lotion (betamethasone dipropionate, 0,55%) were determined by measuring their ability to cause blanching of human skin after topical application. Betnovate and Celestoderm-V lotions produced almost identical blanching profiles. Diprosone lotion displayed a statistically significant superior blanching acitivity over both Betnovate and Celestoderm-V lotions over the whole timespan of the trial.
- Full Text:
Le test de McKenzie-Stoughton: méthode de mesure de l’effect réservoir
- Woodford, R, Haigh, John M, Barry, B W
- Authors: Woodford, R , Haigh, John M , Barry, B W
- Date: 1981
- Language: French
- Type: Article
- Identifier: vital:6448 , http://hdl.handle.net/10962/d1006635
- Description: A la suite de I'observation que firent Mac Kenzie et Stoughton en 1962, montrant que I'application locale de cortico'ldes anti-inflammatoires peut produire un blanchiment de la peau, des tests ont ete mis au point pour apprecier la constitution d'une telle paleur chez des sujets volontaires. Les techniques qui ont ete publiees sont variables quant a la methode d'application (avec ou sans occlusion), la duree d'application du cortico'lde (.c'est-a-dire duree breve de 6 a 8 heures ou prolongee de 16 a 20 heures) et quant a la methode d'appreciation de la reponse, enregistree par exemple comme etant presente ou absente, ou bien cotee, soit avec une simple lecture,. soit avec des lectures repetees, pendant un certain temps. Les auteurs ont mis au point des essais standart de vasoconstriction avec et sans occlusion, pour apprecier les preparations quant a leur efficacite clinique pour determiner la biodisponibilite des cortico'ldes a partir des supports pour applications locales. A condition que I'investigateur et Ie volontaire se conforment strictement au protocole du test les methodes suivantes ont une precision surprenante d'appreciation (Barry et Woodford, 1978).
- Full Text:
- Authors: Woodford, R , Haigh, John M , Barry, B W
- Date: 1981
- Language: French
- Type: Article
- Identifier: vital:6448 , http://hdl.handle.net/10962/d1006635
- Description: A la suite de I'observation que firent Mac Kenzie et Stoughton en 1962, montrant que I'application locale de cortico'ldes anti-inflammatoires peut produire un blanchiment de la peau, des tests ont ete mis au point pour apprecier la constitution d'une telle paleur chez des sujets volontaires. Les techniques qui ont ete publiees sont variables quant a la methode d'application (avec ou sans occlusion), la duree d'application du cortico'lde (.c'est-a-dire duree breve de 6 a 8 heures ou prolongee de 16 a 20 heures) et quant a la methode d'appreciation de la reponse, enregistree par exemple comme etant presente ou absente, ou bien cotee, soit avec une simple lecture,. soit avec des lectures repetees, pendant un certain temps. Les auteurs ont mis au point des essais standart de vasoconstriction avec et sans occlusion, pour apprecier les preparations quant a leur efficacite clinique pour determiner la biodisponibilite des cortico'ldes a partir des supports pour applications locales. A condition que I'investigateur et Ie volontaire se conforment strictement au protocole du test les methodes suivantes ont une precision surprenante d'appreciation (Barry et Woodford, 1978).
- Full Text:
Résultats des études menées en Angleterre: détermination de la posologie optimale
- Woodford, R, Haigh, John M, Barry, B W
- Authors: Woodford, R , Haigh, John M , Barry, B W
- Date: 1981
- Language: French
- Type: Article
- Identifier: vital:6449 , http://hdl.handle.net/10962/d1006636
- Description: Les auteurs ont etudie la biodisponibilite et I'activite de formules experimentales et commercialisees de dermocortico'ides en utilisant les essais de vasoconstriction avec et sans occlusion (1, 2, 6, 7, 8, 9). L'application unique de six heures avec lectures multiples a ete largement utilisee dans ce travail et la methodologie en a ete decrite en detail ailleurs (4). Sur la base de ce travail. on a pratique des applications repetees de dermocortico'ides commercialises au Royaume- Uni. en utilisant Ie test du blanchiment de la peau sans occlusion, de maniere a reproduire au plus pres la situation realisee en clinique (3). Ce travail a montre que I'application de cortico'ides trois fois par jour pendant cinq jours aboutissait a une tachyphylaxie considerable de la reponse du blanchiment quelque soit I'activite therapeutique de la formule utilisee. Au bout de deux jours de repos, on a assiste a une recuperation considerable, mais a nouveau une tachyphylaxie aigue s'est installee a la suite d'une nouvelle application triquotidienne.
- Full Text:
- Authors: Woodford, R , Haigh, John M , Barry, B W
- Date: 1981
- Language: French
- Type: Article
- Identifier: vital:6449 , http://hdl.handle.net/10962/d1006636
- Description: Les auteurs ont etudie la biodisponibilite et I'activite de formules experimentales et commercialisees de dermocortico'ides en utilisant les essais de vasoconstriction avec et sans occlusion (1, 2, 6, 7, 8, 9). L'application unique de six heures avec lectures multiples a ete largement utilisee dans ce travail et la methodologie en a ete decrite en detail ailleurs (4). Sur la base de ce travail. on a pratique des applications repetees de dermocortico'ides commercialises au Royaume- Uni. en utilisant Ie test du blanchiment de la peau sans occlusion, de maniere a reproduire au plus pres la situation realisee en clinique (3). Ce travail a montre que I'application de cortico'ides trois fois par jour pendant cinq jours aboutissait a une tachyphylaxie considerable de la reponse du blanchiment quelque soit I'activite therapeutique de la formule utilisee. Au bout de deux jours de repos, on a assiste a une recuperation considerable, mais a nouveau une tachyphylaxie aigue s'est installee a la suite d'une nouvelle application triquotidienne.
- Full Text:
The release of betamethasone 17-valerate from extemporaneous dilutions of a proprietary topical cream
- Magnus, Ashley D, Haigh, John M, Kanfer, Isadore
- Authors: Magnus, Ashley D , Haigh, John M , Kanfer, Isadore
- Date: 1981
- Language: English
- Type: Article
- Identifier: vital:6397 , http://hdl.handle.net/10962/d1006322
- Description: Six different vehicles for topical use were used to prepare 50% dilutions of Betnovate@ (betamethasone 17-valerate, 0.1 %) cream. Blanching assessment was undertaken immediately after preparing the various dilutions and at 1 and 3 months thereafter. Few statistically significant differences were noted between any of the preparations tested indicating that the rate of release of betamethasone 17-valerate is relatively unaffected by dilution. All preparations were assayed by a stability indicating high pressure liquid chromatographic technique for corticosteroid content. A diminution in the content of betamethasone 17-valerate in the E45 dilution was found 14 months after preparation. All other formulations tested were found to comply with label claim specifications.
- Full Text:
- Authors: Magnus, Ashley D , Haigh, John M , Kanfer, Isadore
- Date: 1981
- Language: English
- Type: Article
- Identifier: vital:6397 , http://hdl.handle.net/10962/d1006322
- Description: Six different vehicles for topical use were used to prepare 50% dilutions of Betnovate@ (betamethasone 17-valerate, 0.1 %) cream. Blanching assessment was undertaken immediately after preparing the various dilutions and at 1 and 3 months thereafter. Few statistically significant differences were noted between any of the preparations tested indicating that the rate of release of betamethasone 17-valerate is relatively unaffected by dilution. All preparations were assayed by a stability indicating high pressure liquid chromatographic technique for corticosteroid content. A diminution in the content of betamethasone 17-valerate in the E45 dilution was found 14 months after preparation. All other formulations tested were found to comply with label claim specifications.
- Full Text:
The simultaneous determination of trimethoprim, sulphamethoxazole and N4-acetylsulphamethoxazole in biological fluids by high pressure liquid chromatography
- Gochin, Rosa, Kanfer, Isadore, Haigh, John M
- Authors: Gochin, Rosa , Kanfer, Isadore , Haigh, John M
- Date: 1981
- Language: English
- Type: text , Article
- Identifier: vital:6364 , http://hdl.handle.net/10962/d1006064
- Description: The simultaneous determination of trimethoprim, sulphamethoxazole and N4-acetylsulphamethoxazole in serum and urine by high-performance liquid chromatography using sulphafurazole as internal standard is described. The separation was achieved on a reversed-phase column employing acetic acid-methanol as the mobile phase with spectrophotometric detection at 230 nm. Precise simultaneous quantitative analysis of the relative components has been achieved at levels of 0.1 μg/ml for both sulphamethoxazole and its N4-acetyl metabolite using 1 ml of serum or urine.
- Full Text:
- Authors: Gochin, Rosa , Kanfer, Isadore , Haigh, John M
- Date: 1981
- Language: English
- Type: text , Article
- Identifier: vital:6364 , http://hdl.handle.net/10962/d1006064
- Description: The simultaneous determination of trimethoprim, sulphamethoxazole and N4-acetylsulphamethoxazole in serum and urine by high-performance liquid chromatography using sulphafurazole as internal standard is described. The separation was achieved on a reversed-phase column employing acetic acid-methanol as the mobile phase with spectrophotometric detection at 230 nm. Precise simultaneous quantitative analysis of the relative components has been achieved at levels of 0.1 μg/ml for both sulphamethoxazole and its N4-acetyl metabolite using 1 ml of serum or urine.
- Full Text:
Assessment of some variables affecting the blanching activity of betamethasone 17-valerate cream
- Magnus, Ashley D, Haigh, John M, Kanfer, Isadore
- Authors: Magnus, Ashley D , Haigh, John M , Kanfer, Isadore
- Date: 1980
- Language: English
- Type: text , Article
- Identifier: vital:6396 , http://hdl.handle.net/10962/d1006320
- Description: The effect of concentration and occlusion time on the ability of Betnovate ® cream (betamethasone 17-valerate 0.1%) to produce skin blanching was assessed. Generally, increased concentration or occlusion time produce and increase in the degree of blanching observed, however, a plateau stage is eventually reached where no further increase of blanching occurs.
- Full Text:
- Authors: Magnus, Ashley D , Haigh, John M , Kanfer, Isadore
- Date: 1980
- Language: English
- Type: text , Article
- Identifier: vital:6396 , http://hdl.handle.net/10962/d1006320
- Description: The effect of concentration and occlusion time on the ability of Betnovate ® cream (betamethasone 17-valerate 0.1%) to produce skin blanching was assessed. Generally, increased concentration or occlusion time produce and increase in the degree of blanching observed, however, a plateau stage is eventually reached where no further increase of blanching occurs.
- Full Text:
Bioavailability and activity of 0.1% amcinonide preparations: comparison with proprietary topical corticosteroid formulations of differing potencies
- Authors: Woodford, R , Haigh, John M
- Date: 1979
- Language: English
- Type: text , Article
- Identifier: vital:6447 , http://hdl.handle.net/10962/d1006634
- Description: The activity of a 0.1% amcinonide cream was compared with those of selected proprietary topical corticosteroid formulations of potencies differing according to the United Kingdom (U.K.) MIMS classification (very potent, potent and moderately potent) using a standard six hour vasoconstrictor assay with multiple reading times. Statistical analysis indicated that 0.1% amcinonide cream feU within the category of a very potent preparation. Three 0.1% amcinonide formulations (cream, combination cream and combination ointment, the last two containing anti-infective agents) were equipotent in the skin-blanching test.
- Full Text:
- Authors: Woodford, R , Haigh, John M
- Date: 1979
- Language: English
- Type: text , Article
- Identifier: vital:6447 , http://hdl.handle.net/10962/d1006634
- Description: The activity of a 0.1% amcinonide cream was compared with those of selected proprietary topical corticosteroid formulations of potencies differing according to the United Kingdom (U.K.) MIMS classification (very potent, potent and moderately potent) using a standard six hour vasoconstrictor assay with multiple reading times. Statistical analysis indicated that 0.1% amcinonide cream feU within the category of a very potent preparation. Three 0.1% amcinonide formulations (cream, combination cream and combination ointment, the last two containing anti-infective agents) were equipotent in the skin-blanching test.
- Full Text:
Comparative blanching activities of proprietary diflucortolone valerate topical preparations
- Coleman, Gerald L, Kanfer, Isadore, Haigh, John M
- Authors: Coleman, Gerald L , Kanfer, Isadore , Haigh, John M
- Date: 1978
- Language: English
- Type: Article
- Identifier: vital:6350 , http://hdl.handle.net/10962/d1006032
- Description: The blanching activities and hence bioavailabilities of the cream, ointment and fatty ointment preparations of Nerisone and Temetex (diflucortolone valerate 0.1%) were evaluated using an occluded and unoccluded blanching assay. These products were compared to Synalar ointment and cream (fluocinolone acetonide 0.025%), established topical corticosteroid preparations. Statistical analysis showed no significant differences between similar formulations of diflucortolone valerate. Significant differences were noted between diflucortolone valerate and fluocinolone acetonide preparations.
- Full Text:
- Authors: Coleman, Gerald L , Kanfer, Isadore , Haigh, John M
- Date: 1978
- Language: English
- Type: Article
- Identifier: vital:6350 , http://hdl.handle.net/10962/d1006032
- Description: The blanching activities and hence bioavailabilities of the cream, ointment and fatty ointment preparations of Nerisone and Temetex (diflucortolone valerate 0.1%) were evaluated using an occluded and unoccluded blanching assay. These products were compared to Synalar ointment and cream (fluocinolone acetonide 0.025%), established topical corticosteroid preparations. Statistical analysis showed no significant differences between similar formulations of diflucortolone valerate. Significant differences were noted between diflucortolone valerate and fluocinolone acetonide preparations.
- Full Text:
The structure of aliphatic amine adducts of uranyl acetylacetonate. II. Dioxobis(2,4-pentanedionato)mono (2-N,N-dimethylaminopentan-4-one)uranium(VI)
- Nassimbeni, L R, Orpen, G, Pauptit, R A, Rodgers, Allen L, Haigh, John M
- Authors: Nassimbeni, L R , Orpen, G , Pauptit, R A , Rodgers, Allen L , Haigh, John M
- Date: 1977
- Language: English
- Type: text , Article
- Identifier: vital:6416 , http://hdl.handle.net/10962/d1006533
- Description: Introduction: In a previous analysis of a compound of this type, we have established that the adduct molecule is bonded through O and that the geometry about U is pentagonal bipyramidal (Haigh, Nassimbeni, Pauptit, Rodgers & Sheldrick, 1976). We have carried out the present analysis to study the conformational effects on the ligand brought about by substitution at N.
- Full Text:
- Authors: Nassimbeni, L R , Orpen, G , Pauptit, R A , Rodgers, Allen L , Haigh, John M
- Date: 1977
- Language: English
- Type: text , Article
- Identifier: vital:6416 , http://hdl.handle.net/10962/d1006533
- Description: Introduction: In a previous analysis of a compound of this type, we have established that the adduct molecule is bonded through O and that the geometry about U is pentagonal bipyramidal (Haigh, Nassimbeni, Pauptit, Rodgers & Sheldrick, 1976). We have carried out the present analysis to study the conformational effects on the ligand brought about by substitution at N.
- Full Text: