- Title
- Amplified fragment length polymorphism confirms reciprocal monophyly in Chrysomya putoria and Chrysomya chloropyga: a correction of reported shared mtDNA haplotypes
- Creator
- Picard, C J, Villet, Martin H, Wells, J D
- Subject
- To be catalogued
- Date
- 2012
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/441760
- Identifier
- vital:73914
- Identifier
- DOI:10.4236/jcdsa.2012.22018
- Description
- Reinvestigation of mitochondrial haplotypes previously reported to be shared between the Afrotropical blowflies Chrysomya putoria Weidemann and Chrysomya chloropyga Weidemann (Diptera: Calliphoridae) revealed an error resulting from the misidentification of specimens. Preliminary amplified fragment length polymorphism (AFLP) analysis of the original and additional individuals again failed to find reciprocal monophyly, leading to a re‐examination of the specimens for diagnostic male genitalic characters that were first described following the earlier study. Four of the original study specimens were found to have been misidentified, and definitive analysis of both mtDNA and AFLP genotypes using phylogenetic analysis and genetic assignment showed that each species was indeed reciprocally monophyletic. In addition to correcting the earlier error, this study illustrates how AFLP analysis can be used for efficient and effective specimen identification through both phylogenetic analysis and genetic assignment, and suggests that the latter method has special advantages for identification when no conspecific specimens are represented in the reference database.
- Format
- 4 pages, pdf
- Language
- English
- Relation
- Medical and Veterinary Entomology, Picard, C.J., Villet, M.H. and Wells, J.D., 2012. Amplified fragment length polymorphism confirms reciprocal monophyly in Chrysomya putoria and Chrysomya chloropyga: a correction of reported shared mtDNA haplotypes. Medical and Veterinary Entomology, 26(1), pp.116-119, Medical and Veterinary Entomology volume 26 number 1 116 119 2012 1365-2915
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Scientific Research Publishing Statement (https://www.scirp.org/ )
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Villet, Martin Herrer (Prof)
SDG 15. Life On Land
RU Department of Zoology and Entomology
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details | SOURCE1 | Amplified fragment length polymorphism confirms reciprocal monophyly in Chrysomya putoria and Chrysomya chloropyga.pdf | 679 KB | Adobe Acrobat PDF | View Details |