ATP-sulphurylase: An enzymatic marker for biological sulphate reduction
- Oyekola, Oluwaseun O, Pletschke, Brett I
- Authors: Oyekola, Oluwaseun O , Pletschke, Brett I
- Date: 2006
- Language: English
- Type: Article
- Identifier: vital:6461 , http://hdl.handle.net/10962/d1005790 , http://dx.doi.org/10.1016/j.soilbio.2006.05.014
- Description: Adenosine triphosphate-sulphurylase (ATPS) plays a major role in dissimilatory sulphate reduction. In this study, the level of ATPS activity was monitored in a time course study using a biosulphidogenic batch bioreactor system. A coincident decrease in ATPS activity with a decline in sulphate concentration and an increase in sulphide concentration as biosulphidogenesis proceeded was observed. Flask studies further showed sulphate to be stimulatory to ATPS, while sulphide proved to be inhibitory. The effect of ions (Ca^(2+), Cl^(−), Fe^(2+) and Zn^(2+)) on the ATPS activity was also investigated. Most of the ions studied (Ca^(2+), Cl^(−) and Fe^(2+)) were stimulatory at lower concentrations (40–120 mg/l) but proved toxic at higher concentrations (>120 mg/l). In contrast, Zn^(2+) was inhibitory even at low concentrations ( 40 mg/l). ATPS may potentially be used as an enzymatic marker for biological sulphate reduction in sulphate-rich wastewaters and natural environments (anaerobic systems such as soils and sediments found in freshwater and marine systems), providing all residual sulphide and interfering ions are removed using a simple preparative step.
- Full Text:
- Authors: Oyekola, Oluwaseun O , Pletschke, Brett I
- Date: 2006
- Language: English
- Type: Article
- Identifier: vital:6461 , http://hdl.handle.net/10962/d1005790 , http://dx.doi.org/10.1016/j.soilbio.2006.05.014
- Description: Adenosine triphosphate-sulphurylase (ATPS) plays a major role in dissimilatory sulphate reduction. In this study, the level of ATPS activity was monitored in a time course study using a biosulphidogenic batch bioreactor system. A coincident decrease in ATPS activity with a decline in sulphate concentration and an increase in sulphide concentration as biosulphidogenesis proceeded was observed. Flask studies further showed sulphate to be stimulatory to ATPS, while sulphide proved to be inhibitory. The effect of ions (Ca^(2+), Cl^(−), Fe^(2+) and Zn^(2+)) on the ATPS activity was also investigated. Most of the ions studied (Ca^(2+), Cl^(−) and Fe^(2+)) were stimulatory at lower concentrations (40–120 mg/l) but proved toxic at higher concentrations (>120 mg/l). In contrast, Zn^(2+) was inhibitory even at low concentrations ( 40 mg/l). ATPS may potentially be used as an enzymatic marker for biological sulphate reduction in sulphate-rich wastewaters and natural environments (anaerobic systems such as soils and sediments found in freshwater and marine systems), providing all residual sulphide and interfering ions are removed using a simple preparative step.
- Full Text:
Cellulases (CMCases) and polyphenol oxidases from thermophilic Bacillus spp
- Mayende, Lungisa, Wilhelmi, Brendan S, Pletschke, Brett I
- Authors: Mayende, Lungisa , Wilhelmi, Brendan S , Pletschke, Brett I
- Date: 2006
- Language: English
- Type: Article
- Identifier: vital:6462 , http://hdl.handle.net/10962/d1005791 , http://dx.doi.org/10.1016/j.soilbio.2006.03.019
- Description: In composting, organic matter is degraded by cellulases and ligninolytic enzymes at temperatures typically above 50 °C. This study isolated thermophilic microorganisms from a compost system that were then screened for cellulase and polyphenol oxidase activity. Temperature optima for the cellulases and polyphenol oxidases were determined as 70 and 40 °C, respectively. Maximal cellulase activity was determined as 1.333 mg glucose released ml[superscript −1] min[superscript −1]. Maximal polyphenol oxidase activity attained was 5.111 nmol phenol ml[superscript −1] min[superscript −1]. Cellulases were found to be stable over a period of 1 h. The isolated compost microorganisms were identified as strains of Bacillus using 16S ribosomal DNA sequence analysis.
- Full Text:
- Authors: Mayende, Lungisa , Wilhelmi, Brendan S , Pletschke, Brett I
- Date: 2006
- Language: English
- Type: Article
- Identifier: vital:6462 , http://hdl.handle.net/10962/d1005791 , http://dx.doi.org/10.1016/j.soilbio.2006.03.019
- Description: In composting, organic matter is degraded by cellulases and ligninolytic enzymes at temperatures typically above 50 °C. This study isolated thermophilic microorganisms from a compost system that were then screened for cellulase and polyphenol oxidase activity. Temperature optima for the cellulases and polyphenol oxidases were determined as 70 and 40 °C, respectively. Maximal cellulase activity was determined as 1.333 mg glucose released ml[superscript −1] min[superscript −1]. Maximal polyphenol oxidase activity attained was 5.111 nmol phenol ml[superscript −1] min[superscript −1]. Cellulases were found to be stable over a period of 1 h. The isolated compost microorganisms were identified as strains of Bacillus using 16S ribosomal DNA sequence analysis.
- Full Text:
- «
- ‹
- 1
- ›
- »