- Title
- Purification and biochemical characterisation of a putative sodium channel agonist secreted from the South African Knobbly sea anemone Bunodosoma capense
- Creator
- van Losenoord, Wynand, Krause, Jason, Parker-Nance, Shirley, Krause, Rui W M, Stoychey, Stoyan, Frost, Carminita L
- Subject
- To be catalogued
- Date
- 2019
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/194082
- Identifier
- vital:45421
- Identifier
- xlink:href="https://doi.org/10.1016/j.toxicon.2019.06.222"
- Description
- Voltage gated ion channels have become a subject of investigation as possible pharmaceutical targets. Research has linked the activity of ion channels directly to anti-inflammatory pathways, energy homeostasis, cancer proliferation and painful diabetic neuropathy. Sea anemones secrete a diverse array of bioactive compounds including potassium and sodium channel toxins. A putative novel sodium channel agonist (molecular mass of 4619.7 Da) with a predicted sequence: CLCNSDGPSV RGNTLSGILW LAGCPSGWHN CKKHKPTIGW CCK was isolated from Bunodosoma capense using a modified stimulation technique to induce the secretion of the neurotoxin rich mucus confirmed by an Artemia nauplii bio-assay. The peptide purification combined size-exclusion and reverse-phase high performance liquid chromatography. A thallium-based ion flux assay confirmed the presence of a sodium channel agonist/inhibitor and purity was determined using a modified tricine SDS-PAGE system. The peptide isolated indicated the presence of multiple disulfide bonds in a tight β-defensin cystine conformation. An IC50 value of 26 nM was determined for total channel inhibition on MCF-7 cells. The unique putative sodium channel agonist initiating with a cystine bond indicates a divergent evolution to those previously isolated from Bunodosoma species.
- Format
- computer, online resource, application/pdf, 1 online resource (11 pages), pdf
- Publisher
- Elsevier
- Language
- English
- Relation
- Toxicon, van Losenoord, W., Krause, J., Parker-Nance, S., Krause, R., Stoychev, S. and Frost, C.L., 2019. Purification and biochemical characterisation of a putative sodium channel agonist secreted from the South African Knobbly sea anemone Bunodosoma capense. Toxicon, 168, pp.147-157, Toxicon volume 168 p. 147 2019 0041-0101
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)
- Rights
- Closed Access
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Krause, Rui Werner Maçedo (Prof)
RU Department of Chemistry
SDG 03. Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details | SOURCE1 | Purification and biochemical characterisation of a putative sodium channel.pdf | 690 KB | Adobe Acrobat PDF | View Details |