Fungal remediation of winery and distillery wastewaters using Trametes pubescens MB 89 and the enhanced production of a high-value enzyme therein
- Authors: Strong, Peter James
- Date: 2008
- Subjects: Fungal remediation Distilleries -- Waste disposal Wine and wine making -- Waste disposal Bioremediation Laccase Enzymes -- Biotechnology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3932 , http://hdl.handle.net/10962/d1003991
- Description: In this study white-rot fungi were investigated for their efficiency at distillery wastewater remediation and the production of laccase as a valuable by-product. Distillery wastewaters are high in organic load and low in pH. The presence of phenolic compounds can lead to extremely colour-rich wastewaters and can be toxic to microorganisms. The presence of the inorganic ions may also affect biological treatment. White-rot fungi are unique among eukaryotic or prokaryotic microbes in possessing powerful oxidative enzyme systems that can degrade lignin to carbon dioxide. These ligninolytic enzymes, such as lignin peroxidase, manganese peroxidase and laccase, are capable of degrading a vast range of toxic, recalcitrant environmental pollutants and this makes the white-rot fungi strong candidates for the bioremediation of polluted soils and waters. The laccase enzyme alone has shown remediation potential in wastewaters such as beer production effluent, olive mill wastewater, alcohol distillery wastes, dye-containing wastewaters from the textile industry as well as wastewaters from the paper and pulp industry. It has been shown to be capable of remediating soils and waters polluted with chlorinated phenolic compounds, polyaromatic hydrocarbons, nitrosubstituted compounds and fungicides, herbicides and insecticides.
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Submerged membrane bioreactor and secondary digestion in the treatment of wine distillery waste: Part II: the effect of fungal pre-treatment on wine distillery wastewater digestion
- Authors: Melamane, Xolisa L , Strong, Peter James , Tandlich, Roman , Burgess, Jo E
- Date: 2007
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/76150 , vital:30514 , https://www.prt-parlar.de/download_feb_2007/
- Description: The effect of fungal pre-treatment using Trametes pubescens on the anaerobic digestion ultrafiltration treatment of wine distillery wastewater (WDW) was studied. The downstream biological treatment system, consisting of four individual reactors, was operated for 30 days. pH buffering was achieved by mixing the pre-treated system feed with CaCO3 and K2HPO4; this proved significant for optimum performance of the system in removal of soluble chemical oxygen demand (CODS). The experimental system was shown to eliminate an average of 86 (± 4) % of CODS present in the pre-treated WDW. Treatment in a submerged membrane bioreactor (SMBR) and subsequent secondary digester, together with pH buffering using CaCO3 and K2HPO4, led to the stabilisation of CODS removal. The residual CODS levels in the final effluent were approximately 400 mg/l, significantly lower than the concentrations observed when treating raw WDW, indicating that fungal pre-treatment might have provided additional nutrients for removal of recalcitrant components of the wastewater. The resulting effluent of the system is rich in nitrates and phosphates. Together with the residual organic content it might be used as a fertiliser. Alternatively, if water management of the wine distillery is an issue, a membrane process, such as reverse osmosis or nanofiltration could be applied to bring the parameters of the water to meet the technological needs.
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