In-situ synthesis of gold nanoparticles on graphene quantum dots-phthalocyanine nanoplatforms: First description of the photophysical and surface enhanced Raman scattering behaviour
- Nwahara, Nnamdi, Achadu, Ojodomo John, Nyokong, Tebello
- Authors: Nwahara, Nnamdi , Achadu, Ojodomo John , Nyokong, Tebello
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/187638 , vital:44682 , xlink:href="https://doi.org/10.1016/j.jphotochem.2018.04.011"
- Description: Owing to the need for new low-dimensional molecular assemblies with tailored electronic properties, the current study presents a facile approach for the synthesis and assembly of gold nanoparticles (AuNPs) onto functional graphene quantum dots (GQDs)-phthalocyanines (Pcs) arrays and the investigation of their photophysical and surface enhanced Raman scattering (SERS) properties. The GQDs were functionalized with L-glutathione (GSH) (to form GQDs@GSH) in order to assist coupling to the low symmetry Zn tris–(tert–butyl) mono carboxyphenoxy (propionic acid) phthalocyanine (complex 1) to form 1@GQDs. The affinity of gold (Au) to sulphur (S) was exploited for the assembly of the AuNPs onto 1@GQDs platform to form 1@GQDs-AuNPs. Transmission electron microscopic investigations confirmed the formation of monodispersed, spherical Pc/GQDs@GSH/AuNPs hybrids. The nanocomposite displayed high triplet quantum yields, which translated into high singlet oxygen quantum yield as high as 87%. Furthermore, the formed composites demonstrated strong surface enhanced Raman scattering (SERS) properties with an unprecedented intrinsic maximal enhancement factor of more than 30-fold. These nanostructures also retain more than 90% of their original SERS intensities after a week of storage, displaying superb stability under ambient conditions. These results highlight the remarkable potential of this composite as a unique Raman-based PDT dosimetric agent.
- Full Text:
- Authors: Nwahara, Nnamdi , Achadu, Ojodomo John , Nyokong, Tebello
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/187638 , vital:44682 , xlink:href="https://doi.org/10.1016/j.jphotochem.2018.04.011"
- Description: Owing to the need for new low-dimensional molecular assemblies with tailored electronic properties, the current study presents a facile approach for the synthesis and assembly of gold nanoparticles (AuNPs) onto functional graphene quantum dots (GQDs)-phthalocyanines (Pcs) arrays and the investigation of their photophysical and surface enhanced Raman scattering (SERS) properties. The GQDs were functionalized with L-glutathione (GSH) (to form GQDs@GSH) in order to assist coupling to the low symmetry Zn tris–(tert–butyl) mono carboxyphenoxy (propionic acid) phthalocyanine (complex 1) to form 1@GQDs. The affinity of gold (Au) to sulphur (S) was exploited for the assembly of the AuNPs onto 1@GQDs platform to form 1@GQDs-AuNPs. Transmission electron microscopic investigations confirmed the formation of monodispersed, spherical Pc/GQDs@GSH/AuNPs hybrids. The nanocomposite displayed high triplet quantum yields, which translated into high singlet oxygen quantum yield as high as 87%. Furthermore, the formed composites demonstrated strong surface enhanced Raman scattering (SERS) properties with an unprecedented intrinsic maximal enhancement factor of more than 30-fold. These nanostructures also retain more than 90% of their original SERS intensities after a week of storage, displaying superb stability under ambient conditions. These results highlight the remarkable potential of this composite as a unique Raman-based PDT dosimetric agent.
- Full Text:
In-situ synthesis of gold nanoparticles on graphene quantum dots-phthalocyanine nanoplatforms: First description of the photophysical and surface enhanced Raman scattering behaviour
- Nwahara, Nnamdi, Achadu, Ojodomo John, Nyokong, Tebello
- Authors: Nwahara, Nnamdi , Achadu, Ojodomo John , Nyokong, Tebello
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/188183 , vital:44730 , xlink:href="https://doi.org/10.1016/j.jphotochem.2018.04.011"
- Description: Owing to the need for new low-dimensional molecular assemblies with tailored electronic properties, the current study presents a facile approach for the synthesis and assembly of gold nanoparticles (AuNPs) onto functional graphene quantum dots (GQDs)-phthalocyanines (Pcs) arrays and the investigation of their photophysical and surface enhanced Raman scattering (SERS) properties. The GQDs were functionalized with L-glutathione (GSH) (to form GQDs@GSH) in order to assist coupling to the low symmetry Zn tris–(tert–butyl) mono carboxyphenoxy (propionic acid) phthalocyanine (complex 1) to form 1@GQDs. The affinity of gold (Au) to sulphur (S) was exploited for the assembly of the AuNPs onto 1@GQDs platform to form 1@GQDs-AuNPs. Transmission electron microscopic investigations confirmed the formation of monodispersed, spherical Pc/GQDs@GSH/AuNPs hybrids. The nanocomposite displayed high triplet quantum yields, which translated into high singlet oxygen quantum yield as high as 87%. Furthermore, the formed composites demonstrated strong surface enhanced Raman scattering (SERS) properties with an unprecedented intrinsic maximal enhancement factor of more than 30-fold. These nanostructures also retain more than 90% of their original SERS intensities after a week of storage, displaying superb stability under ambient conditions. These results highlight the remarkable potential of this composite as a unique Raman-based PDT dosimetric agent.
- Full Text:
- Authors: Nwahara, Nnamdi , Achadu, Ojodomo John , Nyokong, Tebello
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/188183 , vital:44730 , xlink:href="https://doi.org/10.1016/j.jphotochem.2018.04.011"
- Description: Owing to the need for new low-dimensional molecular assemblies with tailored electronic properties, the current study presents a facile approach for the synthesis and assembly of gold nanoparticles (AuNPs) onto functional graphene quantum dots (GQDs)-phthalocyanines (Pcs) arrays and the investigation of their photophysical and surface enhanced Raman scattering (SERS) properties. The GQDs were functionalized with L-glutathione (GSH) (to form GQDs@GSH) in order to assist coupling to the low symmetry Zn tris–(tert–butyl) mono carboxyphenoxy (propionic acid) phthalocyanine (complex 1) to form 1@GQDs. The affinity of gold (Au) to sulphur (S) was exploited for the assembly of the AuNPs onto 1@GQDs platform to form 1@GQDs-AuNPs. Transmission electron microscopic investigations confirmed the formation of monodispersed, spherical Pc/GQDs@GSH/AuNPs hybrids. The nanocomposite displayed high triplet quantum yields, which translated into high singlet oxygen quantum yield as high as 87%. Furthermore, the formed composites demonstrated strong surface enhanced Raman scattering (SERS) properties with an unprecedented intrinsic maximal enhancement factor of more than 30-fold. These nanostructures also retain more than 90% of their original SERS intensities after a week of storage, displaying superb stability under ambient conditions. These results highlight the remarkable potential of this composite as a unique Raman-based PDT dosimetric agent.
- Full Text:
Incorporation of metal free and Ga 5, 10, 15, 20-tetrakis (4-bromophenyl) porphyrin into Pluronic F127-folic acid micelles
- Managa, Muthumuni, Ngoy, Bucolome P, Mafukidze, Donovan M, Nyokong, Tebello
- Authors: Managa, Muthumuni , Ngoy, Bucolome P , Mafukidze, Donovan M , Nyokong, Tebello
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/233256 , vital:50073 , xlink:href="https://doi.org/10.1016/j.jlumin.2017.09.045"
- Description: ClGa 5,10,15,20-Tetrakis (4-bromophenyl) porphyrinato (ClGaTBrPP) and its metal free derivative were successfully synthesized and incorporated into Pluronic F127 polymeric micelles that has been conjugated to folic acid (FA) to form ClGaTBrPP-F127-FA (or H2TBrPP-F127-FA). For comparison, ClGaTBrPP-F127 and H2TBrPP-F127 (without FA) were also formed. The singlet oxygen quantum yield for ClGaTBrPP-F127-FA was higher (ΦΔ = 0.44) than that of H2TBrPP-F127-FA (ΦΔ = 0.37), due to the heavy atom effect of Ga in the former which encourages intersystem crossing to the triplet state. The same applies to ClGaTBrPP-F127 (ΦΔ = 0.47) and H2TBrPP-F127 (ΦΔ = 0.41). Thus, ΦΔ values decreased in the presence of FA, but still high enough for practical application of the nanodrug system. The binding constants Kb were determined to be 1.08 × 104 M−1, 2.51 × 105 M−1, 1.52 × 103 and 2.68 × 103 for H2TBrPP+F127-FA, ClGaTBrPP+F127-FA, ClGaTBrPP-F127 and H2TBrPP-F127, respectively. The Kp values were determined in biphasic octanol and water system.
- Full Text:
- Authors: Managa, Muthumuni , Ngoy, Bucolome P , Mafukidze, Donovan M , Nyokong, Tebello
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/233256 , vital:50073 , xlink:href="https://doi.org/10.1016/j.jlumin.2017.09.045"
- Description: ClGa 5,10,15,20-Tetrakis (4-bromophenyl) porphyrinato (ClGaTBrPP) and its metal free derivative were successfully synthesized and incorporated into Pluronic F127 polymeric micelles that has been conjugated to folic acid (FA) to form ClGaTBrPP-F127-FA (or H2TBrPP-F127-FA). For comparison, ClGaTBrPP-F127 and H2TBrPP-F127 (without FA) were also formed. The singlet oxygen quantum yield for ClGaTBrPP-F127-FA was higher (ΦΔ = 0.44) than that of H2TBrPP-F127-FA (ΦΔ = 0.37), due to the heavy atom effect of Ga in the former which encourages intersystem crossing to the triplet state. The same applies to ClGaTBrPP-F127 (ΦΔ = 0.47) and H2TBrPP-F127 (ΦΔ = 0.41). Thus, ΦΔ values decreased in the presence of FA, but still high enough for practical application of the nanodrug system. The binding constants Kb were determined to be 1.08 × 104 M−1, 2.51 × 105 M−1, 1.52 × 103 and 2.68 × 103 for H2TBrPP+F127-FA, ClGaTBrPP+F127-FA, ClGaTBrPP-F127 and H2TBrPP-F127, respectively. The Kp values were determined in biphasic octanol and water system.
- Full Text:
Influence of annealing on thermoluminescence of natural quartz: kinetic analysis and experimental study of apparent inverse thermal quenching
- Folley, Damilola E, Chithambo, Makaiko L
- Authors: Folley, Damilola E , Chithambo, Makaiko L
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/109996 , vital:33212 , https://doi.org/10.1016/j.radmeas.2018.04.010
- Description: The influence of annealing on the main thermoluminescence glow-peak of natural quartz is reported. For comparison, results from un-annealed quartz are included. The glow-curve measured at 1 °Cs−1 after beta irradiation to 50 Gy revealed six peaks each for quartz annealed at 800 °C for 1 h and the un-annealed sample. The main peak in both quartzes was observed at 72 °C. This report focusses on kinetic analysis of the main peak. The analysis was carried out using various methods consisting of the initial rise, whole glow-peak, peak shape, variable heating rate and phosphorescence-based methods. The activation energy obtained using the various methods ranges between and for the annealed sample and between and for the un-annealed sample. The result suggests that annealing has little effect on the activation energy. The luminescence intensity decreased with heating rate in the un-annealed sample in a manner suggestive of thermal quenching. In contrast, the dependence of intensity on heating rate in the annealed sample is influenced by the dose the sample is irradiated to. Whereas thermal quenching was noted for a dose of 50 Gy in the un-annealed sample, the annealed sample showed evidence of thermal quenching at a low dose of 3 Gy with the opposite effect when irradiated to 50 Gy. The activation energies of thermal quenching were found as and for the un-annealed and annealed samples respectively. We ascribe the apparent dependence of thermal quenching on dose in the annealed sample to competition between radiative and non-radiative transitions at the recombination centre.
- Full Text: false
- Authors: Folley, Damilola E , Chithambo, Makaiko L
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/109996 , vital:33212 , https://doi.org/10.1016/j.radmeas.2018.04.010
- Description: The influence of annealing on the main thermoluminescence glow-peak of natural quartz is reported. For comparison, results from un-annealed quartz are included. The glow-curve measured at 1 °Cs−1 after beta irradiation to 50 Gy revealed six peaks each for quartz annealed at 800 °C for 1 h and the un-annealed sample. The main peak in both quartzes was observed at 72 °C. This report focusses on kinetic analysis of the main peak. The analysis was carried out using various methods consisting of the initial rise, whole glow-peak, peak shape, variable heating rate and phosphorescence-based methods. The activation energy obtained using the various methods ranges between and for the annealed sample and between and for the un-annealed sample. The result suggests that annealing has little effect on the activation energy. The luminescence intensity decreased with heating rate in the un-annealed sample in a manner suggestive of thermal quenching. In contrast, the dependence of intensity on heating rate in the annealed sample is influenced by the dose the sample is irradiated to. Whereas thermal quenching was noted for a dose of 50 Gy in the un-annealed sample, the annealed sample showed evidence of thermal quenching at a low dose of 3 Gy with the opposite effect when irradiated to 50 Gy. The activation energies of thermal quenching were found as and for the un-annealed and annealed samples respectively. We ascribe the apparent dependence of thermal quenching on dose in the annealed sample to competition between radiative and non-radiative transitions at the recombination centre.
- Full Text: false
Influence of knowledge of the end-point on pacing during a 2000m rowing time trial
- Authors: Ferreira, Dean
- Date: 2018
- Subjects: Rowing -- Training , Rowing -- Coaching , Endurance sports -- Training , Rowers -- Ability testing , Rowing -- Training -- Psychological aspects
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/61636 , vital:28044
- Description: Introduction: A typical 2000 m rowing race requires maximal force production over six to eight minutes. Optimal distribution of energetic resources during such a race is vital for optimal performance. There is little research examining the pacing strategies employed by rowers, particularly at the sub-elite level. Aim: The purpose of this investigation was to determine the influence of knowledge of the end-point on pacing during a 2000 m rowing time trial. Methods: Eleven male rowers from a university rowing club volunteered to partake in the study. Each participant completed three experimental trials on an indoor rowing ergometer, each 2000 m. The only difference between the trials was the nature of the information provided beforehand. At the start of the control trial, participants were correctly informed about the distance to be covered. Participants were not informed of the distance to be completed in the unknown trial. For the deceptive trial, participants were told 1000 m would be completed, but when this distance was reached, they were told to continue for another 1000 m. During each trial muscle activity, power output, heart rate, performance time and perceptions of effort were measured.Results: The control trial was significantly (p<0.05) faster than both the unknown and deceptive trials, however the deceptive trial was the fastest of all trials at the 1000 m distance. The unknown trial was slowest at 1000 m and at 2000 m. Muscle activity, RPE, heart rate and power output were significantly (p<0.05) lower in the unknown trial compared to the control and deceptive trial. The control trial exhibited a reverse J-shape pacing profile. The deceptive trial revealed a significant (p<0.05) reduction in performance time, heart rate, power output and muscle activity after the 1000 m interval. The first 500 m interval had the fastest performance and highest power output in all trials. In all trials, RPE was highest at the end of the 2000 m, the greatest reading being obtained at the end of the control trial. During the first 1000 m of the deceptive trial, the dependant variables were similar to those in the control trial. Once the deception was revealed, there was a significant (p<0.05) reduction in the muscle activity, heart rate and power output. Conclusion: The results of this study indicate that accurate end-point information is crucial to the development of an optimal pacing strategy, and ultimately to successful performance. Rowers tend to adopt the reverse J-shape pacing profile most often due to the tactical and physiological benefits offered by this strategy. Unknown or inaccurate end-point information resulted in performance decrements due to the uncertainty associated with the exercise bout.
- Full Text:
- Authors: Ferreira, Dean
- Date: 2018
- Subjects: Rowing -- Training , Rowing -- Coaching , Endurance sports -- Training , Rowers -- Ability testing , Rowing -- Training -- Psychological aspects
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/61636 , vital:28044
- Description: Introduction: A typical 2000 m rowing race requires maximal force production over six to eight minutes. Optimal distribution of energetic resources during such a race is vital for optimal performance. There is little research examining the pacing strategies employed by rowers, particularly at the sub-elite level. Aim: The purpose of this investigation was to determine the influence of knowledge of the end-point on pacing during a 2000 m rowing time trial. Methods: Eleven male rowers from a university rowing club volunteered to partake in the study. Each participant completed three experimental trials on an indoor rowing ergometer, each 2000 m. The only difference between the trials was the nature of the information provided beforehand. At the start of the control trial, participants were correctly informed about the distance to be covered. Participants were not informed of the distance to be completed in the unknown trial. For the deceptive trial, participants were told 1000 m would be completed, but when this distance was reached, they were told to continue for another 1000 m. During each trial muscle activity, power output, heart rate, performance time and perceptions of effort were measured.Results: The control trial was significantly (p<0.05) faster than both the unknown and deceptive trials, however the deceptive trial was the fastest of all trials at the 1000 m distance. The unknown trial was slowest at 1000 m and at 2000 m. Muscle activity, RPE, heart rate and power output were significantly (p<0.05) lower in the unknown trial compared to the control and deceptive trial. The control trial exhibited a reverse J-shape pacing profile. The deceptive trial revealed a significant (p<0.05) reduction in performance time, heart rate, power output and muscle activity after the 1000 m interval. The first 500 m interval had the fastest performance and highest power output in all trials. In all trials, RPE was highest at the end of the 2000 m, the greatest reading being obtained at the end of the control trial. During the first 1000 m of the deceptive trial, the dependant variables were similar to those in the control trial. Once the deception was revealed, there was a significant (p<0.05) reduction in the muscle activity, heart rate and power output. Conclusion: The results of this study indicate that accurate end-point information is crucial to the development of an optimal pacing strategy, and ultimately to successful performance. Rowers tend to adopt the reverse J-shape pacing profile most often due to the tactical and physiological benefits offered by this strategy. Unknown or inaccurate end-point information resulted in performance decrements due to the uncertainty associated with the exercise bout.
- Full Text:
Influence of land cover, proximity to streams and household topographical location on flooding impact in informal settlements in the Eastern Cape, South Africa
- Dalu, Mwazvita T B, Shackleton, Charlie M, Dalu, Tatenda
- Authors: Dalu, Mwazvita T B , Shackleton, Charlie M , Dalu, Tatenda
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/182089 , vital:43799 , xlink:href="https://doi.org/10.1016/j.ijdrr.2017.12.009"
- Description: Patterns of land use, household topographical location and state of natural vegetation influence the spatial distribution of flooding impact. Using field observations and GIS mapping techniques, we investigated how landscape factors influenced structural flooding impact in informal settlements. This study was carried out in the informal settlements of the Eastern Cape Province of South Africa, after the October 2012 floods. Increasing slope significantly raised the probability and level of damage by at least 30% in five of the seven sites, whereas proximity to river and wetlands (more than 100 m) was significant in only two of the sites and at lower levels. Multi-regression analysis highlighted that land cover, proximity to streams and household topographical location influenced the impact of flooding on housing structures. We found that the impact on housing structures in informal settlements during the floods were significantly influenced by their proximity to water bodies, slope factor and patterns of land cover.
- Full Text:
- Authors: Dalu, Mwazvita T B , Shackleton, Charlie M , Dalu, Tatenda
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/182089 , vital:43799 , xlink:href="https://doi.org/10.1016/j.ijdrr.2017.12.009"
- Description: Patterns of land use, household topographical location and state of natural vegetation influence the spatial distribution of flooding impact. Using field observations and GIS mapping techniques, we investigated how landscape factors influenced structural flooding impact in informal settlements. This study was carried out in the informal settlements of the Eastern Cape Province of South Africa, after the October 2012 floods. Increasing slope significantly raised the probability and level of damage by at least 30% in five of the seven sites, whereas proximity to river and wetlands (more than 100 m) was significant in only two of the sites and at lower levels. Multi-regression analysis highlighted that land cover, proximity to streams and household topographical location influenced the impact of flooding on housing structures. We found that the impact on housing structures in informal settlements during the floods were significantly influenced by their proximity to water bodies, slope factor and patterns of land cover.
- Full Text:
Informal economy/sector
- Pillay, Vanessa, Rogan, Michael, Von Broembsen, Marlese
- Authors: Pillay, Vanessa , Rogan, Michael , Von Broembsen, Marlese
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , report
- Identifier: http://hdl.handle.net/10962/477931 , vital:78137 , ISBN
- Description: In response to the 2018 Job Summit priorities of inclusive growth and redistribution and (small and) micro enterprise development, this brief focuses on informal economy. Informal work should not be romanticized–incomes are low for all but a few, while for most working conditions are poor with little security or social protection. However, in South Africa, three in every ten of those who work are informally employed. This is a significant source of employment and, as is the case globally, is increasingly dominant. With unemployment levels increasing (recorded at 27% and 37% for the narrow and expanded definitions in quarter 2 of 2018), the chances of accessing any job in South Africa, whether informal or formal, are currently low.
- Full Text:
- Authors: Pillay, Vanessa , Rogan, Michael , Von Broembsen, Marlese
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , report
- Identifier: http://hdl.handle.net/10962/477931 , vital:78137 , ISBN
- Description: In response to the 2018 Job Summit priorities of inclusive growth and redistribution and (small and) micro enterprise development, this brief focuses on informal economy. Informal work should not be romanticized–incomes are low for all but a few, while for most working conditions are poor with little security or social protection. However, in South Africa, three in every ten of those who work are informally employed. This is a significant source of employment and, as is the case globally, is increasingly dominant. With unemployment levels increasing (recorded at 27% and 37% for the narrow and expanded definitions in quarter 2 of 2018), the chances of accessing any job in South Africa, whether informal or formal, are currently low.
- Full Text:
Informal urban fuelwood markets in South Africa in the context of socio-economic change
- Guild, J, Shackleton, Charlie M
- Authors: Guild, J , Shackleton, Charlie M
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/179779 , vital:43179 , xlink:href="https://doi.org/10.1016/j.enpol.2018.03.023"
- Description: In developing countries, fuelwood is important to livelihoods and energy security because it is used for heating, cooking and can provide additional income to households through trade. The global trade in fuelwood is valued between USD 4–26 billion per annum. In South Africa, fuelwood is a widely used domestic energy source; but how that has changed with rapid urbanisation and socio-economic development over the last two decades is unknown. This study examined the presence and nature of urban fuelwood markets in 39 urban areas of the Eastern Cape and Limpopo provinces. Data were collected from 170 informal fuelwood sellers regarding the prevalence, structure, characteristics, and changes over time of the informal fuelwood markets. Over 80% of the sampled towns had an informal fuelwood market and there was a positive relationship between the number of sellers and town population size. For most sellers, the fuelwood trade was their primary occupation and the income earned was supplemented by government social grants. Sellers earned, on average, over US$9 per day. These results show that despite rapid urbanisation and socio-economic development, fuelwood remains an important domestic fuel in towns and cities of South Africa, and an income source for the urban poor.
- Full Text:
- Authors: Guild, J , Shackleton, Charlie M
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/179779 , vital:43179 , xlink:href="https://doi.org/10.1016/j.enpol.2018.03.023"
- Description: In developing countries, fuelwood is important to livelihoods and energy security because it is used for heating, cooking and can provide additional income to households through trade. The global trade in fuelwood is valued between USD 4–26 billion per annum. In South Africa, fuelwood is a widely used domestic energy source; but how that has changed with rapid urbanisation and socio-economic development over the last two decades is unknown. This study examined the presence and nature of urban fuelwood markets in 39 urban areas of the Eastern Cape and Limpopo provinces. Data were collected from 170 informal fuelwood sellers regarding the prevalence, structure, characteristics, and changes over time of the informal fuelwood markets. Over 80% of the sampled towns had an informal fuelwood market and there was a positive relationship between the number of sellers and town population size. For most sellers, the fuelwood trade was their primary occupation and the income earned was supplemented by government social grants. Sellers earned, on average, over US$9 per day. These results show that despite rapid urbanisation and socio-economic development, fuelwood remains an important domestic fuel in towns and cities of South Africa, and an income source for the urban poor.
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Installation view: Chale Wote Festival 2018
- Authors: Simbao, Ruth K
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/147059 , vital:38589 , https://www.contemporaryand.com/magazines/chale-wote-festival-accra-2018/
- Description: The Chale Wote festival opened on the Day of Re-Membering (20 August), when the Nai Priest poured libations at Brazil House in order to invoke the ancestral spirits. Core events took place on the streets and at various public spaces in James Town from 25 – 26 August. A number of artists including Kiffouly Youchaou, Kresiah Mukwazhi, Va-Bene Elikem Fiatsi (crazinisT artisT), Charlotte Brathwaite, Percy Nii Nortey and the Ubulungiswa/Justice collective created works inside Ussher Fort and James Fort, which were built as slave forts by the Dutch and the British.
- Full Text:
- Authors: Simbao, Ruth K
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/147059 , vital:38589 , https://www.contemporaryand.com/magazines/chale-wote-festival-accra-2018/
- Description: The Chale Wote festival opened on the Day of Re-Membering (20 August), when the Nai Priest poured libations at Brazil House in order to invoke the ancestral spirits. Core events took place on the streets and at various public spaces in James Town from 25 – 26 August. A number of artists including Kiffouly Youchaou, Kresiah Mukwazhi, Va-Bene Elikem Fiatsi (crazinisT artisT), Charlotte Brathwaite, Percy Nii Nortey and the Ubulungiswa/Justice collective created works inside Ussher Fort and James Fort, which were built as slave forts by the Dutch and the British.
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Integrated genetic and morphological data support eco‐evolutionary divergence of Angolan and South African populations of Diplodus hottentotus
- Gwilliam, Michael P, Winkler, Alexander C, Potts, Warren M, Santos, Carmen V D, Sauer, Warwick H H, Shaw, Paul W, McKeown, Niall J
- Authors: Gwilliam, Michael P , Winkler, Alexander C , Potts, Warren M , Santos, Carmen V D , Sauer, Warwick H H , Shaw, Paul W , McKeown, Niall J
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/124833 , vital:35702 , https://doi.10.1111/jfb.13582
- Description: The genus Diplodus presents multiple cases of taxonomic conjecture. Among these the D. cervinus complex was previously described as comprising three subspecies that are now regarded as separate species: Diplodus cervinus, Diplodus hottentotus and Diplodus omanensis. Diplodus hottentotus exhibits a clear break in its distribution around the Benguela Current system, prompting speculation that Angolan and South African populations flanking this area may be isolated and warrant formal taxonomic distinction. This study reports the first integrated genetic [mitochondrial (mt)DNA and nuclear microsatellite] and morphological (morphometric, meristic and colouration) study to assess patterns of divergence between populations in the two regions. High levels of cytonuclear divergence between the populations support a prolonged period of genetic isolation, with the sharing of only one mtDNA haplotype (12 haplotypes were fully sorted between regions) attributed to retention of ancestral polymorphism. Fish from the two regions were significantly differentiated at a number of morphometric (69·5%) and meristic (46%) characters. In addition, Angolan and South African fish exhibited reciprocally diagnostic colouration patterns that were more similar to Mediterranean and Indian Ocean congeners, respectively. Based on the congruent genetic and phenotypic diversity we suggest that the use of hottentotus, whether for full species or subspecies status, should be restricted to South African D. cervinus to reflect their status as a distinct species-like unit, while the relationship between Angolan and Atlantic–Mediterranean D. cervinus will require further demo-genetic analysis. This study highlights the utility of integrated genetic and morphological approaches to assess taxonomic diversity within the biogeographically dynamic Benguela Current region.
- Full Text:
- Authors: Gwilliam, Michael P , Winkler, Alexander C , Potts, Warren M , Santos, Carmen V D , Sauer, Warwick H H , Shaw, Paul W , McKeown, Niall J
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/124833 , vital:35702 , https://doi.10.1111/jfb.13582
- Description: The genus Diplodus presents multiple cases of taxonomic conjecture. Among these the D. cervinus complex was previously described as comprising three subspecies that are now regarded as separate species: Diplodus cervinus, Diplodus hottentotus and Diplodus omanensis. Diplodus hottentotus exhibits a clear break in its distribution around the Benguela Current system, prompting speculation that Angolan and South African populations flanking this area may be isolated and warrant formal taxonomic distinction. This study reports the first integrated genetic [mitochondrial (mt)DNA and nuclear microsatellite] and morphological (morphometric, meristic and colouration) study to assess patterns of divergence between populations in the two regions. High levels of cytonuclear divergence between the populations support a prolonged period of genetic isolation, with the sharing of only one mtDNA haplotype (12 haplotypes were fully sorted between regions) attributed to retention of ancestral polymorphism. Fish from the two regions were significantly differentiated at a number of morphometric (69·5%) and meristic (46%) characters. In addition, Angolan and South African fish exhibited reciprocally diagnostic colouration patterns that were more similar to Mediterranean and Indian Ocean congeners, respectively. Based on the congruent genetic and phenotypic diversity we suggest that the use of hottentotus, whether for full species or subspecies status, should be restricted to South African D. cervinus to reflect their status as a distinct species-like unit, while the relationship between Angolan and Atlantic–Mediterranean D. cervinus will require further demo-genetic analysis. This study highlights the utility of integrated genetic and morphological approaches to assess taxonomic diversity within the biogeographically dynamic Benguela Current region.
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Integrating chemical control with sterile insect releases in an integrated pest management programme for Thaumatotibia leucotreta
- Nepgen, Eugene, Moore, Sean D, Hill, Martin P
- Authors: Nepgen, Eugene , Moore, Sean D , Hill, Martin P
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/423770 , vital:72092 , xlink:href="https://doi.org/10.1111/jen.12487"
- Description: False codling moth Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae) is an important indigenous pest of citrus in southern Africa. Successful control is dependent upon integration of area-wide sterile insect releases and other suppression methods. The aim of this work was to test pyrethroid and organophosphate-based insecticides (tau-fluvalinate and chlorpyrifos) for their residual effect on mortality of released irradiated T. leucotreta male moths. Both of these insecticides were effective in killing irradiated T. leucotreta for 7 days after application on leaves, after which degradation of the active ingredient resulted in a marked reduction in efficacy after 14 days and rendering them harmless. Mortality was found to be similar for irradiated and non-irradiated male T. leucotreta after treatment. Consequently, even though these insecticides might have an effect on moths in the field, ratios of sterile:wild moths should not be altered. Supporting field data from six sites in the Sundays River Valley over a season of sterile insect releases showed the conventional chemical crop protection programme to be as effective as an integrated pest management programme in facilitating effective control of T. leucotreta through sterile insect releases. The study also confirmed that the ratios of sterile:wild male moths in the commercial citrus orchards were not affected by the application of insecticides. These findings confirm the high potential of sterile insect releases for control of T. leucotreta in citrus.
- Full Text:
- Authors: Nepgen, Eugene , Moore, Sean D , Hill, Martin P
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/423770 , vital:72092 , xlink:href="https://doi.org/10.1111/jen.12487"
- Description: False codling moth Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae) is an important indigenous pest of citrus in southern Africa. Successful control is dependent upon integration of area-wide sterile insect releases and other suppression methods. The aim of this work was to test pyrethroid and organophosphate-based insecticides (tau-fluvalinate and chlorpyrifos) for their residual effect on mortality of released irradiated T. leucotreta male moths. Both of these insecticides were effective in killing irradiated T. leucotreta for 7 days after application on leaves, after which degradation of the active ingredient resulted in a marked reduction in efficacy after 14 days and rendering them harmless. Mortality was found to be similar for irradiated and non-irradiated male T. leucotreta after treatment. Consequently, even though these insecticides might have an effect on moths in the field, ratios of sterile:wild moths should not be altered. Supporting field data from six sites in the Sundays River Valley over a season of sterile insect releases showed the conventional chemical crop protection programme to be as effective as an integrated pest management programme in facilitating effective control of T. leucotreta through sterile insect releases. The study also confirmed that the ratios of sterile:wild male moths in the commercial citrus orchards were not affected by the application of insecticides. These findings confirm the high potential of sterile insect releases for control of T. leucotreta in citrus.
- Full Text:
Integration of small-scale mohair farmers into the commercial agricultural economy in Lesotho: a new institutional economics approach
- Authors: Rantlo, Montoeli Ashby
- Date: 2018
- Subjects: Farms, Small Lesotho , Mohair Lesotho , Institutional economics , Transaction costs , Informal sector (Economics)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62275 , vital:28149
- Description: Agriculture continues to be a strategic sector in the development of most low-income countries like Lesotho where small-scale farming is the dominant livelihood activity that provides income and employment to the people. Smallholder farmers' integration into the commercial agricultural markets is a crucial element for economic development and has become a part of development strategies for developing countries and the objectives of international development institutions. The integration of the smallholders into these markets is dependent upon a number of factors including formal and informal institutions. Factors like population growth and demographic changes, technological change and introduction of new commodities, development of infrastructure and market institutions, development of the nonfarm sector and broader economy, rising labour opportunity costs, and macroeconomic, trade and sectoral policies affecting prices and other driving forces determine market participation. In addition, development of input and output markets, institutions like property rights and land tenure, market regulations, cultural and social factors affecting consumption preferences, production and market opportunities and constraints, agro-climatic conditions, and production and market related risks are other factors that affect the commercialisation process. On the other hand, factors like smallholder resource endowments including land and other natural capital, labour, physical capital, and human capital among others are household specific and considered internal determinants of market participation. Nevertheless, the decision to participate in agricultural markets lies with the individual farming household. Under the New Institutional Economics (NIE), this decision is influenced by institutional factors such as risk and preferences, factors which affect household production and the level of costs associated with market transactions. These market transactions are commonly referred to as transaction costs. The study focused on investigating the institutions that limit the integration of small-scale mohair farmers into the commercial agricultural economy in Lesotho. The documentation and evaluation of the institutional structure of the mohair industry is performed whereby the institutional factors influencing participation of small-scale mohair farmers in formal, informal and illegal markets in Lesotho and factors contributing to transaction costs associated with the integration of small-scale farmers into the commercial mohair sector are investigated. Therefore, the investigation will help to address the institutional problems hindering the development of an effective marketing structure for the commercialisation of small-scale mohair producers in Lesotho. The results of the study show that the small-scale mohair farmers that use the formal markets are integrated into the commercial agricultural economy and these farmers' integration into the mainstream economy is influenced by access to government support in the form of shearing sheds, transport subsidies and advisory services. They also have access to market information, marketing infrastructure, knowledge of grades and standards and secure property rights. Other factors that helped their integration into the commercial economy is their contractual agreements with mohair buyers, path dependent based decision making as well as the collective approach to mohair farming. The small-scale mohair farmers using the informal markets do not receive the advantages received by the famers in the formal markets. These small-scale mohair farmers are not integrated into the commercial markets and their only option is the informal markets and their participation in these markets is enhanced by their marketing arrangements with the informal traders, culture influenced decisions, social capital and prompt payments. Despite integration into the commercial agricultural economy, small-scale farmers that use formal markets face the challenges of power imbalances, mistrust and conflicts which may affect this integration into the commercial markets if left unchecked. The study concludes that in the face of institutional challenges, the small-scale mohair farmers using the formal markets are integrated into the commercial agricultural economy and there is potential for improvement of their integration as well as the integration of the small-scale mohair farmers that use the informal markets if institutional challenges are addressed.
- Full Text:
- Authors: Rantlo, Montoeli Ashby
- Date: 2018
- Subjects: Farms, Small Lesotho , Mohair Lesotho , Institutional economics , Transaction costs , Informal sector (Economics)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/62275 , vital:28149
- Description: Agriculture continues to be a strategic sector in the development of most low-income countries like Lesotho where small-scale farming is the dominant livelihood activity that provides income and employment to the people. Smallholder farmers' integration into the commercial agricultural markets is a crucial element for economic development and has become a part of development strategies for developing countries and the objectives of international development institutions. The integration of the smallholders into these markets is dependent upon a number of factors including formal and informal institutions. Factors like population growth and demographic changes, technological change and introduction of new commodities, development of infrastructure and market institutions, development of the nonfarm sector and broader economy, rising labour opportunity costs, and macroeconomic, trade and sectoral policies affecting prices and other driving forces determine market participation. In addition, development of input and output markets, institutions like property rights and land tenure, market regulations, cultural and social factors affecting consumption preferences, production and market opportunities and constraints, agro-climatic conditions, and production and market related risks are other factors that affect the commercialisation process. On the other hand, factors like smallholder resource endowments including land and other natural capital, labour, physical capital, and human capital among others are household specific and considered internal determinants of market participation. Nevertheless, the decision to participate in agricultural markets lies with the individual farming household. Under the New Institutional Economics (NIE), this decision is influenced by institutional factors such as risk and preferences, factors which affect household production and the level of costs associated with market transactions. These market transactions are commonly referred to as transaction costs. The study focused on investigating the institutions that limit the integration of small-scale mohair farmers into the commercial agricultural economy in Lesotho. The documentation and evaluation of the institutional structure of the mohair industry is performed whereby the institutional factors influencing participation of small-scale mohair farmers in formal, informal and illegal markets in Lesotho and factors contributing to transaction costs associated with the integration of small-scale farmers into the commercial mohair sector are investigated. Therefore, the investigation will help to address the institutional problems hindering the development of an effective marketing structure for the commercialisation of small-scale mohair producers in Lesotho. The results of the study show that the small-scale mohair farmers that use the formal markets are integrated into the commercial agricultural economy and these farmers' integration into the mainstream economy is influenced by access to government support in the form of shearing sheds, transport subsidies and advisory services. They also have access to market information, marketing infrastructure, knowledge of grades and standards and secure property rights. Other factors that helped their integration into the commercial economy is their contractual agreements with mohair buyers, path dependent based decision making as well as the collective approach to mohair farming. The small-scale mohair farmers using the informal markets do not receive the advantages received by the famers in the formal markets. These small-scale mohair farmers are not integrated into the commercial markets and their only option is the informal markets and their participation in these markets is enhanced by their marketing arrangements with the informal traders, culture influenced decisions, social capital and prompt payments. Despite integration into the commercial agricultural economy, small-scale farmers that use formal markets face the challenges of power imbalances, mistrust and conflicts which may affect this integration into the commercial markets if left unchecked. The study concludes that in the face of institutional challenges, the small-scale mohair farmers using the formal markets are integrated into the commercial agricultural economy and there is potential for improvement of their integration as well as the integration of the small-scale mohair farmers that use the informal markets if institutional challenges are addressed.
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Interaction of catechol O-methyltransferase with gold and silver nanoparticles
- Authors: Usman, Aminu
- Date: 2018
- Subjects: Parkinson's disease , Methyltransferases , Catechol , Nanoparticles
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/61818 , vital:28063 , DOI https://doi.org/10.21504/10962/61818
- Description: Catechol O-methyltransferase (S-adenosyl-Z-methionine: catechol O-methyltransferase; COMT; EC 2.1.1.6) is a ubiquitous enzyme that catalyses the transfer of a methyl group from the cofactor, S-adenosyl-Z-methionine (SAM) to a hydroxyl group of endogenous and exogenous catechol-containing moieties. The physiological role of this enzyme is the methylation and thereby inactivation of the catechol-containing bio-active and bio-toxic compounds, including catechol-neurotransmitters, catechol-estrogens and catechol-containing drugs. Activity of this enzyme is implicated in the treatment of Parkinson’s disease and is associated with other diseases including breast cancer and an array neuropsychological disorders, such as schizophrenia. This thesis explores the use of gold and silver nanoparticles (NPs) (AuNPs and AgNPs) to inhibit the catalytic activity of mammalian COMT. Because of its accessibility and availability, we initially investigated bovine soluble COMT (BSCOMT) from liver tissue. Bioinformatic analyses and structural modeling revealed high (>90%) sequence similarity between BSCOMT and human soluble COMT (HSCOMT). BSCOMT was partially purified to 7.78 fold, 1.65% yield and had a specific activity of 0.052 U/mg. It had pH and temperature optima of 8.5 and 40oC, respectively. The Km, Vmax, Kcat and Kcat/Km towards esculetin methylation were respectively 1.475±0.130 pM, 0.0353±0.001 pmol/ml/min, 1.748 x 10-2±5.0x10-4 min-1 and 1.18x10-2 M-1. min-1. HSCOMT was expressed in Escherichia coli BL21(DE3) which showed optimal activity for esculetin methylation at pH and temperature of 7.0 and 30°C, respectively. It was purified to 5.62 fold, 22.6% yield with a specific activity of 3.85 U/mg. HSCOMT kinetic plots, upon incubation of the reaction mixture at 30°C for 5 min before addition of SAM was hyperbolic with Km, Vmax, Kcat and Kcat/Km values of 1.79 pM, 0.412 pmol/ml/min, 2.08 min-1 and 1.165 M-1. min-1, respectively. AuNPs and AgNPs showed a concentration dependent inhibition of HSCOMT activity upon increasing the 5 min incubation time to 1 h. Interestingly, HSCOMT kinetics, with 1 h incubation at 30°C, showed a sigmoidal curve, as well as increased activity. Incubation of the reaction mixture in the presence of 60 pM AuNPs and/or AgNPs for 1 hreversed the observed sigmoidal to a hyperbolic curve, with kinetic parameters comparable to those of 5 min incubation. SDS-PAGE analyses of HSCOMT after the kinetic experiments showed the enzyme incubated for 5 min as a monomer, while that which was incubated for 1 h migrated substantially as dimer. However, the HSCOMT incubated for 1 h in the presence of 60 pM AuNPs and/or AgNPs migrated as a monomer. This indicated that the extension of the incubation period allowed the dimerization of HSCOMT, which exhibited sigmoidal kinetics and higher activity. The presence of NPs impeded the HSCOMT dimerization which decreased the activity. Varying the concentration of SAM suggested that SAM had an allosteric modulatory effect on HSCOMT. Absorption spectroscopy indicated adsorption of HSCOMT on the gold and silver NP surfaces and the formation of NPs-HSCOMT corona. Fluorescence spectroscopy showed that the interaction of HSCOMT with both gold and silver NPs was governed by a static quenching mechanism, implying the formation of a non-fluorescent fluorophore-NP complex at the ground state. Further fluorometric analyses indicated that both gold and silver NPs had contact with Trp143; that the interactions were spontaneous and were driven by electrostatic interactions. Fourier transform infrared spectroscopic studies showed the adsorption of HSCOMT of the NPs surfaces to cause relaxation of the enzyme’s B-sheet structures. Molecular docking studies indicated involvement of largely hydrophilic amino acids, with the interacting distances of less than 3.5A. These findings signify the potential of nanotechnology in the control of COMT catalytic activity for the management of the COMT-related disorders. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2018
- Full Text:
- Authors: Usman, Aminu
- Date: 2018
- Subjects: Parkinson's disease , Methyltransferases , Catechol , Nanoparticles
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/61818 , vital:28063 , DOI https://doi.org/10.21504/10962/61818
- Description: Catechol O-methyltransferase (S-adenosyl-Z-methionine: catechol O-methyltransferase; COMT; EC 2.1.1.6) is a ubiquitous enzyme that catalyses the transfer of a methyl group from the cofactor, S-adenosyl-Z-methionine (SAM) to a hydroxyl group of endogenous and exogenous catechol-containing moieties. The physiological role of this enzyme is the methylation and thereby inactivation of the catechol-containing bio-active and bio-toxic compounds, including catechol-neurotransmitters, catechol-estrogens and catechol-containing drugs. Activity of this enzyme is implicated in the treatment of Parkinson’s disease and is associated with other diseases including breast cancer and an array neuropsychological disorders, such as schizophrenia. This thesis explores the use of gold and silver nanoparticles (NPs) (AuNPs and AgNPs) to inhibit the catalytic activity of mammalian COMT. Because of its accessibility and availability, we initially investigated bovine soluble COMT (BSCOMT) from liver tissue. Bioinformatic analyses and structural modeling revealed high (>90%) sequence similarity between BSCOMT and human soluble COMT (HSCOMT). BSCOMT was partially purified to 7.78 fold, 1.65% yield and had a specific activity of 0.052 U/mg. It had pH and temperature optima of 8.5 and 40oC, respectively. The Km, Vmax, Kcat and Kcat/Km towards esculetin methylation were respectively 1.475±0.130 pM, 0.0353±0.001 pmol/ml/min, 1.748 x 10-2±5.0x10-4 min-1 and 1.18x10-2 M-1. min-1. HSCOMT was expressed in Escherichia coli BL21(DE3) which showed optimal activity for esculetin methylation at pH and temperature of 7.0 and 30°C, respectively. It was purified to 5.62 fold, 22.6% yield with a specific activity of 3.85 U/mg. HSCOMT kinetic plots, upon incubation of the reaction mixture at 30°C for 5 min before addition of SAM was hyperbolic with Km, Vmax, Kcat and Kcat/Km values of 1.79 pM, 0.412 pmol/ml/min, 2.08 min-1 and 1.165 M-1. min-1, respectively. AuNPs and AgNPs showed a concentration dependent inhibition of HSCOMT activity upon increasing the 5 min incubation time to 1 h. Interestingly, HSCOMT kinetics, with 1 h incubation at 30°C, showed a sigmoidal curve, as well as increased activity. Incubation of the reaction mixture in the presence of 60 pM AuNPs and/or AgNPs for 1 hreversed the observed sigmoidal to a hyperbolic curve, with kinetic parameters comparable to those of 5 min incubation. SDS-PAGE analyses of HSCOMT after the kinetic experiments showed the enzyme incubated for 5 min as a monomer, while that which was incubated for 1 h migrated substantially as dimer. However, the HSCOMT incubated for 1 h in the presence of 60 pM AuNPs and/or AgNPs migrated as a monomer. This indicated that the extension of the incubation period allowed the dimerization of HSCOMT, which exhibited sigmoidal kinetics and higher activity. The presence of NPs impeded the HSCOMT dimerization which decreased the activity. Varying the concentration of SAM suggested that SAM had an allosteric modulatory effect on HSCOMT. Absorption spectroscopy indicated adsorption of HSCOMT on the gold and silver NP surfaces and the formation of NPs-HSCOMT corona. Fluorescence spectroscopy showed that the interaction of HSCOMT with both gold and silver NPs was governed by a static quenching mechanism, implying the formation of a non-fluorescent fluorophore-NP complex at the ground state. Further fluorometric analyses indicated that both gold and silver NPs had contact with Trp143; that the interactions were spontaneous and were driven by electrostatic interactions. Fourier transform infrared spectroscopic studies showed the adsorption of HSCOMT of the NPs surfaces to cause relaxation of the enzyme’s B-sheet structures. Molecular docking studies indicated involvement of largely hydrophilic amino acids, with the interacting distances of less than 3.5A. These findings signify the potential of nanotechnology in the control of COMT catalytic activity for the management of the COMT-related disorders. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2018
- Full Text:
Interactions between three biological control agents of water hyacinth, Eichhornia crassipes (Mart.) Solms (Pontederiaceae) in South Africa
- Authors: Petela, Nomvume
- Date: 2018
- Subjects: Water hyacinth -- South Africa , Water hyacinth -- Biological control -- South Africa , Aquatic weeds -- Biological control -- South Africa , Curculionidae , Delphacidae , Miridae , Neochetina eichhorniae Warner , Megamelus scutellaris Berg , Eccritotarsus eichhorniae Henry
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/60676 , vital:27814
- Description: Water hyacinth, Eichhomia crassipes (Mart.) Solms (Pontederiaceae) is a free-floating perennial weed that is regarded as the worst aquatic weed in the world because of its negative impacts on aquatic ecosystems. It is native to the Amazon Basin of South America, but since the late 1800s has spread throughout the world. The first record of the weed in South Africa was noted in 1908 on the Cape Flats and in KwaZulu-Natal, but it is now dispersed throughout the country. Mechanical and chemical control methods have been used against the weed, but biological control is considered the most cost-effective, sustainable and environmentally friendly intervention. Currently, nine biological control agents have been released against water hyacinth in South Africa, and Neochetina eichhorniae Warner (Coleoptera: Curculionidae) is used most widely to control it. However, in some sites, water hyacinth mats have still not been brought under control because of eutrophic waters and cool temperatures. It was therefore necessary to release new biological control agents to complement the impact of N. eichhorniae. Megamelus scutellaris Berg (Hemiptera: Delphacidae) was released in 2013, but little is known about how it interacts with other agents already present in South Africa. It is likely to compete with the established biological control agent, Eccritotarsus eichhorniae Henry (Heteroptera: Miridae), because they are both sap suckers. On the other hand, N. eichhorniae is the most widespread and thus the most important biological control agent for water hyacinth. The aim of this study, then, was to determine the interactions between the two sap-sucking agents in South Africa that presumably occupy similar niches on the plant, and the interaction between M. scutellerais and N. eichhorniae, the most widely distributed and abundant agent in South Africa. Three experiments were conducted at the Waainek Research Facility at Rhodes University, Grahamstown, Eastern Cape, South Africa. Plants were grown for two weeks and insect species were inoculated singly or in combination. Water hyacinth, plant growth parameters and insect parameters were measured every 14 days for a period of 12 weeks. The results of the study showed that feeding by either E. eichhorniae or M. scutellaris had no effect on the feeding of the other agent. Both agents reduced all the measured plant growth parameters equally, either singly or in combination (i.e. E. eichhorniae or M. scutellaris alone or together). The interaction between the two agents appears neutral and agents are likely to complement each other in the field. Prior feeding by E. eichhorniae or M. scutellaris on water hyacinth did not affect the subsequent feeding by either agent. Megamelus scutellaris prefers healthy fresh water hyacinth plants. In addition, planthoppers performed best in combination with the weevil, especially on plants with new weevil feeding scars. The results of the study showed that M. scutellaris is compatible with other biological control agents of water hyacinth that are already established in South Africa. Therefore, the introduction of M. scutellaris may enhance the biological control of water hyacinth in South Africa.
- Full Text:
- Authors: Petela, Nomvume
- Date: 2018
- Subjects: Water hyacinth -- South Africa , Water hyacinth -- Biological control -- South Africa , Aquatic weeds -- Biological control -- South Africa , Curculionidae , Delphacidae , Miridae , Neochetina eichhorniae Warner , Megamelus scutellaris Berg , Eccritotarsus eichhorniae Henry
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/60676 , vital:27814
- Description: Water hyacinth, Eichhomia crassipes (Mart.) Solms (Pontederiaceae) is a free-floating perennial weed that is regarded as the worst aquatic weed in the world because of its negative impacts on aquatic ecosystems. It is native to the Amazon Basin of South America, but since the late 1800s has spread throughout the world. The first record of the weed in South Africa was noted in 1908 on the Cape Flats and in KwaZulu-Natal, but it is now dispersed throughout the country. Mechanical and chemical control methods have been used against the weed, but biological control is considered the most cost-effective, sustainable and environmentally friendly intervention. Currently, nine biological control agents have been released against water hyacinth in South Africa, and Neochetina eichhorniae Warner (Coleoptera: Curculionidae) is used most widely to control it. However, in some sites, water hyacinth mats have still not been brought under control because of eutrophic waters and cool temperatures. It was therefore necessary to release new biological control agents to complement the impact of N. eichhorniae. Megamelus scutellaris Berg (Hemiptera: Delphacidae) was released in 2013, but little is known about how it interacts with other agents already present in South Africa. It is likely to compete with the established biological control agent, Eccritotarsus eichhorniae Henry (Heteroptera: Miridae), because they are both sap suckers. On the other hand, N. eichhorniae is the most widespread and thus the most important biological control agent for water hyacinth. The aim of this study, then, was to determine the interactions between the two sap-sucking agents in South Africa that presumably occupy similar niches on the plant, and the interaction between M. scutellerais and N. eichhorniae, the most widely distributed and abundant agent in South Africa. Three experiments were conducted at the Waainek Research Facility at Rhodes University, Grahamstown, Eastern Cape, South Africa. Plants were grown for two weeks and insect species were inoculated singly or in combination. Water hyacinth, plant growth parameters and insect parameters were measured every 14 days for a period of 12 weeks. The results of the study showed that feeding by either E. eichhorniae or M. scutellaris had no effect on the feeding of the other agent. Both agents reduced all the measured plant growth parameters equally, either singly or in combination (i.e. E. eichhorniae or M. scutellaris alone or together). The interaction between the two agents appears neutral and agents are likely to complement each other in the field. Prior feeding by E. eichhorniae or M. scutellaris on water hyacinth did not affect the subsequent feeding by either agent. Megamelus scutellaris prefers healthy fresh water hyacinth plants. In addition, planthoppers performed best in combination with the weevil, especially on plants with new weevil feeding scars. The results of the study showed that M. scutellaris is compatible with other biological control agents of water hyacinth that are already established in South Africa. Therefore, the introduction of M. scutellaris may enhance the biological control of water hyacinth in South Africa.
- Full Text:
Interrogating teacher leadership development through a formative intervention: a case study in a rural Secondary School in northern Namibia
- Authors: Iyambo, David Kandiwapa
- Date: 2018
- Subjects: Educational leadership -- Namibia , School management and organization -- Namibia , Teachers -- Training of -- Namibia , Cultural Historical Activity Theory
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/61547 , vital:28035
- Description: The Namibian education system has undergone major policy shifts from a ‘top-down’ hierarchical leadership practice to a more shared and democratic form of leadership in schools. These policies compel principals and school management team members to involve level-one teachers in decision-making and other leadership roles within their schools and beyond. However, to this end, the goals envisaged by policies for teachers to participate in, and contribute to the overall school leadership activities and decision-making have not been fully realised. This was due to the inherent hierarchy of the ‘top-down’ system and autocratic leadership style which remains powerful within the current school practice. Against this backdrop, this study interrogated how teacher leadership can be developed in a rural Secondary School in northern Namibia. The underlying cultural-historical conditions that promoted or constrained teacher leadership development were surfaced. Opportunities for changes in leadership practices through a formative intervention were developed. Cultural Historical Activity Theory (CHAT) was utilised as a theoretical and analytical framework in this study together with Grant’s Model of Teacher Leadership (2006; 2008; 2010). Five level-one teachers, two school management members and a school board chairperson were selected as research participants by means of a purposive sampling method. Furthermore, the study used document analysis, semi-structured interviews, questionnaires and change laboratory workshops as main tools for data generation. The findings revealed that participants understood the concept of teacher leadership differently and that teachers in the case study school were leading in all four zones of teacher leadership model (Grant, 2006; 2008; 2012) although their roles differed. However, the study also found that teacher leadership development was mostly intensified by managerial structures. It appeared from the findings of this study that conditions such as the role of the school management team (SMT) members in promoting teacher leadership development, a supportive organisational culture, and provision of learning support amongst staff members through the attendance of workshops emerged as factors promoting the development of teachers as leaders. The study also revealed that there were many cultural and historical tensions that constrained the practice of teacher leadership development in school. Thus, the study argues that limited leadership training and an inherent ‘top-down’ hierarchical style of leadership was the main underlying systemic causes that constrained teachers to be developed as leaders. Through the change laboratory workshops, the findings suggested that there was a need for continuous professional development initiatives and leadership training, as alternative way for the realisation of teacher leadership development. Finally, a recommendation that leadership aspects should be constituted in pre-and in-service professional development training as an ongoing practice is made.
- Full Text:
- Authors: Iyambo, David Kandiwapa
- Date: 2018
- Subjects: Educational leadership -- Namibia , School management and organization -- Namibia , Teachers -- Training of -- Namibia , Cultural Historical Activity Theory
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/61547 , vital:28035
- Description: The Namibian education system has undergone major policy shifts from a ‘top-down’ hierarchical leadership practice to a more shared and democratic form of leadership in schools. These policies compel principals and school management team members to involve level-one teachers in decision-making and other leadership roles within their schools and beyond. However, to this end, the goals envisaged by policies for teachers to participate in, and contribute to the overall school leadership activities and decision-making have not been fully realised. This was due to the inherent hierarchy of the ‘top-down’ system and autocratic leadership style which remains powerful within the current school practice. Against this backdrop, this study interrogated how teacher leadership can be developed in a rural Secondary School in northern Namibia. The underlying cultural-historical conditions that promoted or constrained teacher leadership development were surfaced. Opportunities for changes in leadership practices through a formative intervention were developed. Cultural Historical Activity Theory (CHAT) was utilised as a theoretical and analytical framework in this study together with Grant’s Model of Teacher Leadership (2006; 2008; 2010). Five level-one teachers, two school management members and a school board chairperson were selected as research participants by means of a purposive sampling method. Furthermore, the study used document analysis, semi-structured interviews, questionnaires and change laboratory workshops as main tools for data generation. The findings revealed that participants understood the concept of teacher leadership differently and that teachers in the case study school were leading in all four zones of teacher leadership model (Grant, 2006; 2008; 2012) although their roles differed. However, the study also found that teacher leadership development was mostly intensified by managerial structures. It appeared from the findings of this study that conditions such as the role of the school management team (SMT) members in promoting teacher leadership development, a supportive organisational culture, and provision of learning support amongst staff members through the attendance of workshops emerged as factors promoting the development of teachers as leaders. The study also revealed that there were many cultural and historical tensions that constrained the practice of teacher leadership development in school. Thus, the study argues that limited leadership training and an inherent ‘top-down’ hierarchical style of leadership was the main underlying systemic causes that constrained teachers to be developed as leaders. Through the change laboratory workshops, the findings suggested that there was a need for continuous professional development initiatives and leadership training, as alternative way for the realisation of teacher leadership development. Finally, a recommendation that leadership aspects should be constituted in pre-and in-service professional development training as an ongoing practice is made.
- Full Text:
Introduction to the special issue: applied critical realism in the social sciences
- Authors: Price, Leigh , Martin, Lee
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/392133 , vital:68724 , xlink:href="https://doi.org/10.1080/14767430.2018.1468148"
- Description: The aim of our initial call for papers was to encourage the submission of exemplars of applied work, reflections on the use of critical realism, and metatheoretical developments. We were not disappointed, and we are therefore pleased to present this collection of five articles which advance our understanding of critical realism in practice. The book review in this issue further extends the collection, as it summarizes several examples of applied critical realist work. As one would expect of such a collection, there are a variety of disciplines represented, from business studies, to marketing, psychology, law and education. In this editorial, we provide an overview of the (concrete universal) trends of current applications of critical realism of which these articles are (concrete singular, and therefore unique) instantiations. Finally, we provide a brief introduction to each paper. We expect that the audience for this issue may be broader than, though still include, the usual readership of Journal of Critical Realism. Specifically, we expect to attract early career researchers who are new to critical realist ideas, and people whose primary interest is directed at one of the disciplines represented, rather than critical realism per se. For this reason, at the risk of repetition, we have allowed several of the authors to outline the aspects of critical realism that are relevant to their paper.
- Full Text:
- Authors: Price, Leigh , Martin, Lee
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/392133 , vital:68724 , xlink:href="https://doi.org/10.1080/14767430.2018.1468148"
- Description: The aim of our initial call for papers was to encourage the submission of exemplars of applied work, reflections on the use of critical realism, and metatheoretical developments. We were not disappointed, and we are therefore pleased to present this collection of five articles which advance our understanding of critical realism in practice. The book review in this issue further extends the collection, as it summarizes several examples of applied critical realist work. As one would expect of such a collection, there are a variety of disciplines represented, from business studies, to marketing, psychology, law and education. In this editorial, we provide an overview of the (concrete universal) trends of current applications of critical realism of which these articles are (concrete singular, and therefore unique) instantiations. Finally, we provide a brief introduction to each paper. We expect that the audience for this issue may be broader than, though still include, the usual readership of Journal of Critical Realism. Specifically, we expect to attract early career researchers who are new to critical realist ideas, and people whose primary interest is directed at one of the disciplines represented, rather than critical realism per se. For this reason, at the risk of repetition, we have allowed several of the authors to outline the aspects of critical realism that are relevant to their paper.
- Full Text:
Introduction: Blurring Boundaries
- Mnyaka, Phindezwa, Macleod, Catriona I
- Authors: Mnyaka, Phindezwa , Macleod, Catriona I
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , book chapter
- Identifier: http://hdl.handle.net/10962/434252 , vital:73042 , ISBN 978-3-319-74720-0 , https://link.springer.com/chapter/10.1007/978-3-319-74721-7_9
- Description: This chapter introduces the section on blurring boundaries in research. The chapters within this section unpack the ethical dilemmas that researchers negotiate when they find themselves stepping outside their roles as researchers in the field. Research encounters presuppose particular boundaries, depending on the methodology employed and the research questions posed. However, participants may identify researchers differently, compelling them to respond in unanticipated ways as possibly therapists, clinicians, interlocutors, and activists. Collectively, the authors of the chapters in this section explore the ethical implications of blurring such boundaries. They unpack the significance of various forms of intimacies that may emerge while undertaking research, how researchers are positioned as agents of social change, and how researchers negotiate their statuses as insiders and outsiders in fieldwork.
- Full Text:
- Authors: Mnyaka, Phindezwa , Macleod, Catriona I
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , book chapter
- Identifier: http://hdl.handle.net/10962/434252 , vital:73042 , ISBN 978-3-319-74720-0 , https://link.springer.com/chapter/10.1007/978-3-319-74721-7_9
- Description: This chapter introduces the section on blurring boundaries in research. The chapters within this section unpack the ethical dilemmas that researchers negotiate when they find themselves stepping outside their roles as researchers in the field. Research encounters presuppose particular boundaries, depending on the methodology employed and the research questions posed. However, participants may identify researchers differently, compelling them to respond in unanticipated ways as possibly therapists, clinicians, interlocutors, and activists. Collectively, the authors of the chapters in this section explore the ethical implications of blurring such boundaries. They unpack the significance of various forms of intimacies that may emerge while undertaking research, how researchers are positioned as agents of social change, and how researchers negotiate their statuses as insiders and outsiders in fieldwork.
- Full Text:
Introduction: On (still) rediscovering the ordinary
- Authors: Wessels, Paul
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/458513 , vital:75750 , https://hdl.handle.net/10520/EJC-1061aba2b5
- Description: The essays included in this special issue of English in Africa are written by published writers of fiction and poetry who have close ties to academic departments as teachers, writers, scholars and editors. While they are scholarly in form, these articles seek to evoke a broader range of response than the purely academic, and for this reason they may not always strictly follow academic conventions. For example, to preserve the flow of the discussion parenthetical references may, at times, have been excluded, and ‘creative’ poetry and prose are often drawn into the argument for effect. We hope that the tone of the keynote address, while it has been abridged and edited for print, remains conversational and anecdotal, in keeping with its oral delivery.
- Full Text:
- Authors: Wessels, Paul
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/458513 , vital:75750 , https://hdl.handle.net/10520/EJC-1061aba2b5
- Description: The essays included in this special issue of English in Africa are written by published writers of fiction and poetry who have close ties to academic departments as teachers, writers, scholars and editors. While they are scholarly in form, these articles seek to evoke a broader range of response than the purely academic, and for this reason they may not always strictly follow academic conventions. For example, to preserve the flow of the discussion parenthetical references may, at times, have been excluded, and ‘creative’ poetry and prose are often drawn into the argument for effect. We hope that the tone of the keynote address, while it has been abridged and edited for print, remains conversational and anecdotal, in keeping with its oral delivery.
- Full Text:
Introduction: The politics of anonymity and confidentiality
- Macleod, Catriona I, Mnyaka, Phindezwa
- Authors: Macleod, Catriona I , Mnyaka, Phindezwa
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , book chapter
- Identifier: http://hdl.handle.net/10962/434237 , vital:73041 , ISBN 978-3-319-74720-0 , https://link.springer.com/chapter/10.1007/978-3-319-74721-7_15
- Description: Ethics committees standardly require that the researchers address questions concerning anonymity and confidentiality. The conventional practice is to ensure that participants’ names and identifying details are expunged from public records of the research and that high levels of confidentiality of data are maintained in the research process. In this introduction, we outline how authors of chapters in this section ask questions concerning these imperatives, including circumstances where participants actively want their identity revealed and their voice heard or when anonymising might not be possible or may further disadvantage marginalised populations. We explore the argument made by authors that the automatic anonymising of data and the imposition of confidentiality can constrain ethical conduct.
- Full Text:
- Authors: Macleod, Catriona I , Mnyaka, Phindezwa
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , book chapter
- Identifier: http://hdl.handle.net/10962/434237 , vital:73041 , ISBN 978-3-319-74720-0 , https://link.springer.com/chapter/10.1007/978-3-319-74721-7_15
- Description: Ethics committees standardly require that the researchers address questions concerning anonymity and confidentiality. The conventional practice is to ensure that participants’ names and identifying details are expunged from public records of the research and that high levels of confidentiality of data are maintained in the research process. In this introduction, we outline how authors of chapters in this section ask questions concerning these imperatives, including circumstances where participants actively want their identity revealed and their voice heard or when anonymising might not be possible or may further disadvantage marginalised populations. We explore the argument made by authors that the automatic anonymising of data and the imposition of confidentiality can constrain ethical conduct.
- Full Text:
Invaded habitat incompatibility affects the suitability of the potential biological control agent Listronotus sordidus for Sagittaria platyphylla in South Africa
- Martin, Grant D, Coetzee, Julie A, Lloyd, Melissa, Nombewu, Sinoxolo E, Ndlovu, Mpilonhle S, Kwong, Raelene M
- Authors: Martin, Grant D , Coetzee, Julie A , Lloyd, Melissa , Nombewu, Sinoxolo E , Ndlovu, Mpilonhle S , Kwong, Raelene M
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/103926 , vital:32323 , https://doi.org/10.1080/09583157.2018.1460314
- Description: Sagittaria platyphylla (Engelmann) J.G. Smith (Alismataceae) was first recorded in South Africa in 2008 and is considered to be an emerging weed with naturalised populations occurring throughout the country. A biological control programme was initiated in Australia and surveys conducted between 2010 and 2012 yielded potential agents, including the crown feeding weevil, Listronotus sordidus Gyllenhal (Coleoptera: Curculionidae). The potential of L. sordidus as a candidate biological control agent against S. platyphylla in South Africa was examined. Although adult feeding was recorded on a number of plant species, oviposition and larval development indicated a narrow host range restricted to the Alismataceae. In South Africa, S. platyphylla populations are primarily found in inundated systems. However, laboratory studies showed that L. sordidus did not oviposit on inundated plants, potentially nullifying the impact of the insect on South African populations. It is suggested that even though L. sordidus is a damaging, specific agent, its limited impact on inundated plant populations in South Africa does not justify the inherent risk associated with the release of a biological control agent.
- Full Text: false
- Authors: Martin, Grant D , Coetzee, Julie A , Lloyd, Melissa , Nombewu, Sinoxolo E , Ndlovu, Mpilonhle S , Kwong, Raelene M
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/103926 , vital:32323 , https://doi.org/10.1080/09583157.2018.1460314
- Description: Sagittaria platyphylla (Engelmann) J.G. Smith (Alismataceae) was first recorded in South Africa in 2008 and is considered to be an emerging weed with naturalised populations occurring throughout the country. A biological control programme was initiated in Australia and surveys conducted between 2010 and 2012 yielded potential agents, including the crown feeding weevil, Listronotus sordidus Gyllenhal (Coleoptera: Curculionidae). The potential of L. sordidus as a candidate biological control agent against S. platyphylla in South Africa was examined. Although adult feeding was recorded on a number of plant species, oviposition and larval development indicated a narrow host range restricted to the Alismataceae. In South Africa, S. platyphylla populations are primarily found in inundated systems. However, laboratory studies showed that L. sordidus did not oviposit on inundated plants, potentially nullifying the impact of the insect on South African populations. It is suggested that even though L. sordidus is a damaging, specific agent, its limited impact on inundated plant populations in South Africa does not justify the inherent risk associated with the release of a biological control agent.
- Full Text: false