Computational design for genome reduction of Lactococcus lactis towards microbial chassis development
- Authors: Hamese, Saltiel
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435321 , vital:73147
- Description: Access restricted. Expected release date 2026. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2024
- Full Text:
- Authors: Hamese, Saltiel
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435321 , vital:73147
- Description: Access restricted. Expected release date 2026. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2024
- Full Text:
English morphological awareness and reading comprehension in deaf and hearing grade 3 to 7 learners from Lesotho
- Authors: Tšehla, Puleng Magret
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/${Handle} , vital:73169
- Description: There is a noticeable literacy crisis observed in both Deaf and hearing learners from Lesotho. This study investigates the English Morphological Awareness and reading comprehension of 26 Deaf and 82 hearing learners enrolled in grades 3 to 7 in two schools in Lesotho. This study employs a correlational cross-sectional quantitative design. Each participant completed two literacy assessment tasks: a reading comprehension task and a Morphological Awareness task. The Morphological Awareness task encompassed five subtasks that assessed the learners’ inflectional, derivational, and compound awareness. The results of these assessments are analysed through appropriate statistical analyses. In addition, errors made by the Deaf and hearing learners on the literacy assessments are compared and analysed. This analysis determines the types of errors made by each group and identifies the factors that influence these errors. The performance of both groups in terms of task scores, in general, was low. Deaf learners and hearing learners’ performance on the tasks was similar. This outcome contradicts most previous studies, which indicate that Deaf learners typically exhibit lower levels of Morphological Awareness development and reading comprehension compared to their hearing counterparts. The results of this study also suggest that there is a statistically significant relationship between Morphological Awareness and reading comprehension in both groups. Finally, Deaf and hearing learners made similar errors on the tasks. There was some evidence of influence from both the Deaf and hearing learners’ first languages (Sesotho and Lesotho Sign Language, respectively). The study demonstrates the need for more explicit morphological instruction to improve both Deaf and hearing learners’ literacy. , Thesis (MA) -- Faculty of Humanities, Linguistics and Applied Language Studies, 2024
- Full Text:
- Authors: Tšehla, Puleng Magret
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/${Handle} , vital:73169
- Description: There is a noticeable literacy crisis observed in both Deaf and hearing learners from Lesotho. This study investigates the English Morphological Awareness and reading comprehension of 26 Deaf and 82 hearing learners enrolled in grades 3 to 7 in two schools in Lesotho. This study employs a correlational cross-sectional quantitative design. Each participant completed two literacy assessment tasks: a reading comprehension task and a Morphological Awareness task. The Morphological Awareness task encompassed five subtasks that assessed the learners’ inflectional, derivational, and compound awareness. The results of these assessments are analysed through appropriate statistical analyses. In addition, errors made by the Deaf and hearing learners on the literacy assessments are compared and analysed. This analysis determines the types of errors made by each group and identifies the factors that influence these errors. The performance of both groups in terms of task scores, in general, was low. Deaf learners and hearing learners’ performance on the tasks was similar. This outcome contradicts most previous studies, which indicate that Deaf learners typically exhibit lower levels of Morphological Awareness development and reading comprehension compared to their hearing counterparts. The results of this study also suggest that there is a statistically significant relationship between Morphological Awareness and reading comprehension in both groups. Finally, Deaf and hearing learners made similar errors on the tasks. There was some evidence of influence from both the Deaf and hearing learners’ first languages (Sesotho and Lesotho Sign Language, respectively). The study demonstrates the need for more explicit morphological instruction to improve both Deaf and hearing learners’ literacy. , Thesis (MA) -- Faculty of Humanities, Linguistics and Applied Language Studies, 2024
- Full Text:
Yield curve and business cycle dynamics in South Africa: new evidence from a Markov switching model
- Authors: Rotich, Mercyline Chepkemoi
- Date: 2024-04-03
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434739 , vital:73101
- Description: Globally, several empirical studies have demonstrated the ability of the yield spread to predict a recession in a country. In South Africa, previous studies have not only shown the yield curve's predictive power but have further demonstrated that it outperforms other commonly used variables, such as the growth rate of real money supply, changes in stock prices, and the index of leading economic indicators. However, some recent studies have shown that the yield spread (the spread between 10-year bonds and 3-month Treasury bills) gave false signals of recession. In this study, we explore the possible reasons for the false signals of the yield spread by addressing the following questions. Does the yield spread used matter? Does the measure of the business cycle used matter? And do the estimation techniques used matter? To address the first question, unlike the previous studies, this paper uses four different yield spreads- depicting short-term, medium-term, and long-term government bonds against the backdrop of a changing structure of bond holding, which reflects the increasing risk eversion of investors in South Africa. Second, the paper used different measures of business cycles, namely industrial production index, lagging, coincident, and leading economic indicators. The empirical models were estimated using both univariate and multivariate Markov switching models. As economic theory suggests, the univariate Markov switching model was used to determine if each variable exhibits a significant regime switching. The multivariate Markov switching model was estimated for each business cycle and yield spread variable, with each of the other variables serving as a non-switching explanatory variable, thereby addressing potential endogeneity concerns and the predictive power of the explanatory variable. Finally, the multivariate Markov switching model was estimated for three monthly sample periods, a full sample for 1986 to 2022, and two sub-samples – 1986 to 2009 and 2010 to 2022. This analysis consistently reveals significant regime-switching behavior across all the series thus, affirming the superiority of the regime switching model over the standard model used in previous studies. By analyzing the transition probabilities and the expected durations between these regimes, we find that including the spreads in the business cycle model improves the models’ predictability, with the medium-term bonds spread performing better than the usual long-term spread. The smoothed regime probability of the best-performing models is compared with the SARB recession dates; the two closely resemble each other, proving that the Markov switching model can help predict the turning points in the business cycle in South Africa. , Thesis (MCom) -- Faculty of Commerce, Economics and Economic History, 2024
- Full Text:
- Authors: Rotich, Mercyline Chepkemoi
- Date: 2024-04-03
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434739 , vital:73101
- Description: Globally, several empirical studies have demonstrated the ability of the yield spread to predict a recession in a country. In South Africa, previous studies have not only shown the yield curve's predictive power but have further demonstrated that it outperforms other commonly used variables, such as the growth rate of real money supply, changes in stock prices, and the index of leading economic indicators. However, some recent studies have shown that the yield spread (the spread between 10-year bonds and 3-month Treasury bills) gave false signals of recession. In this study, we explore the possible reasons for the false signals of the yield spread by addressing the following questions. Does the yield spread used matter? Does the measure of the business cycle used matter? And do the estimation techniques used matter? To address the first question, unlike the previous studies, this paper uses four different yield spreads- depicting short-term, medium-term, and long-term government bonds against the backdrop of a changing structure of bond holding, which reflects the increasing risk eversion of investors in South Africa. Second, the paper used different measures of business cycles, namely industrial production index, lagging, coincident, and leading economic indicators. The empirical models were estimated using both univariate and multivariate Markov switching models. As economic theory suggests, the univariate Markov switching model was used to determine if each variable exhibits a significant regime switching. The multivariate Markov switching model was estimated for each business cycle and yield spread variable, with each of the other variables serving as a non-switching explanatory variable, thereby addressing potential endogeneity concerns and the predictive power of the explanatory variable. Finally, the multivariate Markov switching model was estimated for three monthly sample periods, a full sample for 1986 to 2022, and two sub-samples – 1986 to 2009 and 2010 to 2022. This analysis consistently reveals significant regime-switching behavior across all the series thus, affirming the superiority of the regime switching model over the standard model used in previous studies. By analyzing the transition probabilities and the expected durations between these regimes, we find that including the spreads in the business cycle model improves the models’ predictability, with the medium-term bonds spread performing better than the usual long-term spread. The smoothed regime probability of the best-performing models is compared with the SARB recession dates; the two closely resemble each other, proving that the Markov switching model can help predict the turning points in the business cycle in South Africa. , Thesis (MCom) -- Faculty of Commerce, Economics and Economic History, 2024
- Full Text:
Constructions of Ukuyalwa and marriage satisfaction: experiences of Xhosa couples in the Eastern Cape
- Authors: Bikwe, Siphuxolo
- Date: 2023-03-30
- Subjects: Xhosa (African people) South Africa Eastern Cape , Xhosa (African people) Marriage customs and rites , Black psychology , Couples therapy , Interpersonal relations and culture , Communication in marriage , Satisfaction , Foucauldian discourse analysis
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408805 , vital:70527
- Description: Marital satisfaction may be described as the extent to which one’s expectations, desires and needs are being satisfied in their marriage. Studies that concern marital satisfaction provide us with crucial insights into factors that support or compromise the longevity of long-term, committed relationships. Little work has been done in understanding the role of cultural practices in marital satisfaction particularly in Africa, and South Africa more specifically. Ukuyalwa is a Xhosa ritual that takes place during a Xhosa traditional wedding, whereby the bride is introduced to the (traditional) guiding practices of her new home by the women of the family together with her sisters-in-law. This study informs the practice of African psychology as formulated by Ratele (2017b).Critical African psychology as a framework recognizes the construction of culture alongside political, socioeconomic, and historical matrices. These are acknowledged as an important element in psychological theories, methods, and explanations. This research project aimed to identify the discourses that circulate around ukuyalwa as a Xhosa cultural practice and consider the implications for the marital satisfaction of amaXhosa couples. The study interviewed 3 Xhosa couples of varying ages who had been married for different durations to develop an understanding of their experiences of ukuyalwa and marital satisfaction. Data was analyzed using a Foucauldian Discourse Analysis (FDA) which allowed for the identifications of the discourses that the couples used to construct ukuyalwa and marital satisfaction. The couples discursively constructed marriage as ‘divine unity’, ‘unification of families’ and ‘partnership’. Ukuyalwa was constructed as a ‘gendered process’, ideas of tradition and modernity, and a ‘foundation’ also became apparent. Satisfaction was constructed as ‘open communication’ and ‘work’. The couples’ positions shifted in their conversations, where in some instances they were 'learners’, ‘initiates’, and/or ‘passive’, and in other instances, they were ‘in charge’, ‘knowledgeable’, and ‘experts’. The discourses of culture, religion, and partnership circulated prominently in relation to marriage, ukuyalwa, and marital satisfaction. The findings of the study speak to how the couples’ constructions of marriage and cultural customs such as ukuyalwa are valuable as they often inform how couples behave in their marriage relationships and what they regard as satisfactory to them when it comes to marriage. These insights into what informs the couples’ ideas on marriage and satisfaction can be utilised in informing couples therapy interventions. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
- Full Text:
- Authors: Bikwe, Siphuxolo
- Date: 2023-03-30
- Subjects: Xhosa (African people) South Africa Eastern Cape , Xhosa (African people) Marriage customs and rites , Black psychology , Couples therapy , Interpersonal relations and culture , Communication in marriage , Satisfaction , Foucauldian discourse analysis
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408805 , vital:70527
- Description: Marital satisfaction may be described as the extent to which one’s expectations, desires and needs are being satisfied in their marriage. Studies that concern marital satisfaction provide us with crucial insights into factors that support or compromise the longevity of long-term, committed relationships. Little work has been done in understanding the role of cultural practices in marital satisfaction particularly in Africa, and South Africa more specifically. Ukuyalwa is a Xhosa ritual that takes place during a Xhosa traditional wedding, whereby the bride is introduced to the (traditional) guiding practices of her new home by the women of the family together with her sisters-in-law. This study informs the practice of African psychology as formulated by Ratele (2017b).Critical African psychology as a framework recognizes the construction of culture alongside political, socioeconomic, and historical matrices. These are acknowledged as an important element in psychological theories, methods, and explanations. This research project aimed to identify the discourses that circulate around ukuyalwa as a Xhosa cultural practice and consider the implications for the marital satisfaction of amaXhosa couples. The study interviewed 3 Xhosa couples of varying ages who had been married for different durations to develop an understanding of their experiences of ukuyalwa and marital satisfaction. Data was analyzed using a Foucauldian Discourse Analysis (FDA) which allowed for the identifications of the discourses that the couples used to construct ukuyalwa and marital satisfaction. The couples discursively constructed marriage as ‘divine unity’, ‘unification of families’ and ‘partnership’. Ukuyalwa was constructed as a ‘gendered process’, ideas of tradition and modernity, and a ‘foundation’ also became apparent. Satisfaction was constructed as ‘open communication’ and ‘work’. The couples’ positions shifted in their conversations, where in some instances they were 'learners’, ‘initiates’, and/or ‘passive’, and in other instances, they were ‘in charge’, ‘knowledgeable’, and ‘experts’. The discourses of culture, religion, and partnership circulated prominently in relation to marriage, ukuyalwa, and marital satisfaction. The findings of the study speak to how the couples’ constructions of marriage and cultural customs such as ukuyalwa are valuable as they often inform how couples behave in their marriage relationships and what they regard as satisfactory to them when it comes to marriage. These insights into what informs the couples’ ideas on marriage and satisfaction can be utilised in informing couples therapy interventions. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
- Full Text:
Evaluating the trophic ecology and feeding habits of three divergent lineages of Sandelia bainsii (Teleostei: Anabantidae), from the Eastern Cape Rivers using stable isotope analysis
- Authors: Nkomo, Thulisile
- Date: 2022-10-14
- Subjects: Anabantidae , Trophic ecology , Food chains (Ecology) , Food web , Freshwater fishes Food , Stable isotopes
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/364984 , vital:65667
- Description: Despite supporting a disproportionately large fraction of the global biodiversity, freshwater ecosystems are ranked as the most highly threatened habitats on the planet, ahead of both terrestrial and marine ecosystems. Many regions are still characterised by limited knowledge regarding the taxonomy and ecology of freshwater fish taxa. The need for ecological information is increasingly becoming important due to the discovery of new species and unique lineages, which require conservation management. The aim of this study was thus to evaluate the trophic ecology and feeding habits of the three recently described and divergent Sandelia bainsii lineages, namely Sandelia sp. ‘bainsii Kowie’ from the Great Fish River, Sandelia sp. ‘bainsii Keiskamma’ found in the Keiskamma River, and Sandelia sp. ‘bainsii Buffalo’ confined to the Buffalo River system in the Amathole-Winterberg freshwater ecoregion in the Eastern Cape Province, South Africa. Based on the allopatric distribution and the generalist feeding habits of these lineages, this thesis postulated that these three lineages were likely to show similar trophic ecology patterns, different dietary composition and as a result would have variable trophic positioning in the different river systems. Therefore, the primary objectives were to use stable isotope analysis to (1) evaluate the food web patterns of the river systems where the three S. bainsii lineages occurred, and (2) determine dietary source contributions for the three lineages using isotope mixing models. The results revealed general variability and significant differences in the δ13C and δ¹⁵N values for the different basal resources, macroinvertebrates and fish community across the different headwater streams. Within and across the different rivers, the S. bainsii lineages exhibited variable isotopic niche sizes, which appeared to coincide with the variation in the isotopic composition of the individual communities. Furthermore, these lineages did not exhibit any discernible patterns in their interspecific interactions in different habitats. This suggests that these lineages’ isotopic niche patterns were largely influenced by spatial differences in both trophic resources and probable interactions with contraspecifics. Assessment of trophic positions of S. bainsii lineages showed that the three lineages had higher trophic positions than other co-occurring species at most sites, except in the Buffalo River. This suggest that the different lineages were generally top predators in the different river systems. Although S. ‘bainsii Buffalo’ had a lower trophic position compared to other co-occurring species, its trophic position was generally characterised by high uncertainty, indicating that this lineage was likely influenced by the occurrence of diet sources that had highly variable stable isotope values. Findings from stable isotope mixing models revealed that the diet sources varied from the dominance of either single diet source in the Fairburn and Tyume 1 River to the importance of multiple prey sources from the Lushington and Kat River system. This suggests that despite being a top predator at most sites, the diet sources for the different lineages were highly variable. The patterns observed in this study did not appear to be attributed to species divergence possibly caused by allopatric speciation, but rather differences in food web characteristics of the river systems, as well as the lineages interspecific relationships and their generalized feeding strategies. Understanding the trophic dynamics of these lineages will assist in implementing effective conservation strategies and policies dealing with narrowly distributed species that are threatened by habitat fragmentation and invasion of piscivorous fish. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2022
- Full Text:
- Authors: Nkomo, Thulisile
- Date: 2022-10-14
- Subjects: Anabantidae , Trophic ecology , Food chains (Ecology) , Food web , Freshwater fishes Food , Stable isotopes
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/364984 , vital:65667
- Description: Despite supporting a disproportionately large fraction of the global biodiversity, freshwater ecosystems are ranked as the most highly threatened habitats on the planet, ahead of both terrestrial and marine ecosystems. Many regions are still characterised by limited knowledge regarding the taxonomy and ecology of freshwater fish taxa. The need for ecological information is increasingly becoming important due to the discovery of new species and unique lineages, which require conservation management. The aim of this study was thus to evaluate the trophic ecology and feeding habits of the three recently described and divergent Sandelia bainsii lineages, namely Sandelia sp. ‘bainsii Kowie’ from the Great Fish River, Sandelia sp. ‘bainsii Keiskamma’ found in the Keiskamma River, and Sandelia sp. ‘bainsii Buffalo’ confined to the Buffalo River system in the Amathole-Winterberg freshwater ecoregion in the Eastern Cape Province, South Africa. Based on the allopatric distribution and the generalist feeding habits of these lineages, this thesis postulated that these three lineages were likely to show similar trophic ecology patterns, different dietary composition and as a result would have variable trophic positioning in the different river systems. Therefore, the primary objectives were to use stable isotope analysis to (1) evaluate the food web patterns of the river systems where the three S. bainsii lineages occurred, and (2) determine dietary source contributions for the three lineages using isotope mixing models. The results revealed general variability and significant differences in the δ13C and δ¹⁵N values for the different basal resources, macroinvertebrates and fish community across the different headwater streams. Within and across the different rivers, the S. bainsii lineages exhibited variable isotopic niche sizes, which appeared to coincide with the variation in the isotopic composition of the individual communities. Furthermore, these lineages did not exhibit any discernible patterns in their interspecific interactions in different habitats. This suggests that these lineages’ isotopic niche patterns were largely influenced by spatial differences in both trophic resources and probable interactions with contraspecifics. Assessment of trophic positions of S. bainsii lineages showed that the three lineages had higher trophic positions than other co-occurring species at most sites, except in the Buffalo River. This suggest that the different lineages were generally top predators in the different river systems. Although S. ‘bainsii Buffalo’ had a lower trophic position compared to other co-occurring species, its trophic position was generally characterised by high uncertainty, indicating that this lineage was likely influenced by the occurrence of diet sources that had highly variable stable isotope values. Findings from stable isotope mixing models revealed that the diet sources varied from the dominance of either single diet source in the Fairburn and Tyume 1 River to the importance of multiple prey sources from the Lushington and Kat River system. This suggests that despite being a top predator at most sites, the diet sources for the different lineages were highly variable. The patterns observed in this study did not appear to be attributed to species divergence possibly caused by allopatric speciation, but rather differences in food web characteristics of the river systems, as well as the lineages interspecific relationships and their generalized feeding strategies. Understanding the trophic dynamics of these lineages will assist in implementing effective conservation strategies and policies dealing with narrowly distributed species that are threatened by habitat fragmentation and invasion of piscivorous fish. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2022
- Full Text:
The implementation of a mobile application to decrease occupational sitting through goal setting and social comparison
- Authors: Tsaoane, Moipone Lipalesa
- Date: 2022-10-14
- Subjects: Sedentary behavior , Sitting position , Feedback , Mobile apps , Behavior modification , Agile software development
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/365544 , vital:65758
- Description: Background: Feedback proves to be a valuable tool in behaviour change as it is said to increase compliance and improve the effectiveness of interventions. Interventions that focus on decreasing sedentary behaviour as an independent factor from physical activity are necessary, especially for office workers who spend most of their day seated. There is insufficient knowledge regarding the effectiveness of feedback as a tool to decrease sedentary behaviour. This project implemented a tool that can be used to determine this. To take advantage of the cost-effectiveness and scalability of digital technologies, a mobile application was selected as the mode of delivery. Method: The application was designed as an intervention, using the Theoretical Domains Framework. It was then implemented into a fully functioning application through an agile development process, using Xam- arin.Forms framework. Due to challenges with this framework, a second application was developed using the React Native framework. Pilot studies were used for testing, with the final one consisting of Rhodes University employees. Results: The Xamarin.Forms application proved to be unfeasible; some users experienced fatal errors and crashes. The React Native application worked as desired and produced accurate and consistent step count readings, proving feasible from a functionality standpoint. The agile methodology enabled the developer to focus on implementing and testing one component at a time, which made the development process more manageable. Conclusion: Future work must conduct empirical studies to determine if feedback is an effective tool compared to a control group and which type of feedback (between goal-setting and social comparison) is most effective. , Thesis (MSc) -- Faculty of Science, Computer Science, 2022
- Full Text:
- Authors: Tsaoane, Moipone Lipalesa
- Date: 2022-10-14
- Subjects: Sedentary behavior , Sitting position , Feedback , Mobile apps , Behavior modification , Agile software development
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/365544 , vital:65758
- Description: Background: Feedback proves to be a valuable tool in behaviour change as it is said to increase compliance and improve the effectiveness of interventions. Interventions that focus on decreasing sedentary behaviour as an independent factor from physical activity are necessary, especially for office workers who spend most of their day seated. There is insufficient knowledge regarding the effectiveness of feedback as a tool to decrease sedentary behaviour. This project implemented a tool that can be used to determine this. To take advantage of the cost-effectiveness and scalability of digital technologies, a mobile application was selected as the mode of delivery. Method: The application was designed as an intervention, using the Theoretical Domains Framework. It was then implemented into a fully functioning application through an agile development process, using Xam- arin.Forms framework. Due to challenges with this framework, a second application was developed using the React Native framework. Pilot studies were used for testing, with the final one consisting of Rhodes University employees. Results: The Xamarin.Forms application proved to be unfeasible; some users experienced fatal errors and crashes. The React Native application worked as desired and produced accurate and consistent step count readings, proving feasible from a functionality standpoint. The agile methodology enabled the developer to focus on implementing and testing one component at a time, which made the development process more manageable. Conclusion: Future work must conduct empirical studies to determine if feedback is an effective tool compared to a control group and which type of feedback (between goal-setting and social comparison) is most effective. , Thesis (MSc) -- Faculty of Science, Computer Science, 2022
- Full Text:
Individual decision-making and cooperation in freshwater fisheries management at the Somme River, northern France
- Authors: Khumalo, Brian
- Date: 2022-04-07
- Subjects: Fishery management France Somme River , Fisheries France Somme River , Human ecology France Somme River , Traditional ecological knowledge France Somme River , Decision making , Experimental economics , Recreation France Somme River
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/294561 , vital:57233
- Description: Are altruistic individuals more likely to cooperate when exploiting common resources? This research study asks whether or not rates of human altruistic behaviour expressed by individual recreational fishers in interpersonal contexts at the Somme River, Amiens mirror those rates of altruism expressed in collective contexts concerning local fisheries resources. In a natural resources context, altruism manifests as a willingness by fishers to incur personal cost for common-pool resource benefit. Accordingly, it is understood that altruistic behaviour reflected collectively expresses itself as cooperation. The research study takes place in Northern France with the stated objectives to: 1) observe individual fishers’ altruistic propensities in interpersonal contexts involving other fishers, 2) observe individual rates of altruistic behaviour in collective contexts involving common fisheries resources and compare with those expressed interpersonally, and 3) investigate whether or not a local (informal) management system existed in the town of Amiens to better understand if informal tenure of water space influences altruistic behaviour or not. The research design consists of two components, one quantitative and one qualitative. The former employs two economic games; a Dictators Games (DG) and a Public Good Game (PGG) in service of the first and second research objectives, and the latter employs cognitive mapping and free-listing exercises in service of the third. Here economic games stand as proxies for real-world situations involving individual (DG) and collective (PGG) decision-making whereas the exercises seek to uncover local ecological knowledge (LEK). The results found that while individual recreational fishers demonstrated lower rates of interpersonal altruism overall, in a collective setting involving local fisheries resources the rate was higher, implying a greater willingness to incur personal cost. Ecological knowledge was high among experienced fishers, yet no knowledge pertaining to parallel management and or informal rules of exclusion or resource subtraction were observed, suggesting an informal management system did not exist. The study additionally documents freshwater biodiversity, providing an index of fish species present in the river collected from the free-listing exercises, categorized into native and non-native as the latter can negatively affect trophic systems and ecosystem processes. , Thesis (MSocSci) -- Faculty of Humanities, Anthropology, 2022
- Full Text:
- Authors: Khumalo, Brian
- Date: 2022-04-07
- Subjects: Fishery management France Somme River , Fisheries France Somme River , Human ecology France Somme River , Traditional ecological knowledge France Somme River , Decision making , Experimental economics , Recreation France Somme River
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/294561 , vital:57233
- Description: Are altruistic individuals more likely to cooperate when exploiting common resources? This research study asks whether or not rates of human altruistic behaviour expressed by individual recreational fishers in interpersonal contexts at the Somme River, Amiens mirror those rates of altruism expressed in collective contexts concerning local fisheries resources. In a natural resources context, altruism manifests as a willingness by fishers to incur personal cost for common-pool resource benefit. Accordingly, it is understood that altruistic behaviour reflected collectively expresses itself as cooperation. The research study takes place in Northern France with the stated objectives to: 1) observe individual fishers’ altruistic propensities in interpersonal contexts involving other fishers, 2) observe individual rates of altruistic behaviour in collective contexts involving common fisheries resources and compare with those expressed interpersonally, and 3) investigate whether or not a local (informal) management system existed in the town of Amiens to better understand if informal tenure of water space influences altruistic behaviour or not. The research design consists of two components, one quantitative and one qualitative. The former employs two economic games; a Dictators Games (DG) and a Public Good Game (PGG) in service of the first and second research objectives, and the latter employs cognitive mapping and free-listing exercises in service of the third. Here economic games stand as proxies for real-world situations involving individual (DG) and collective (PGG) decision-making whereas the exercises seek to uncover local ecological knowledge (LEK). The results found that while individual recreational fishers demonstrated lower rates of interpersonal altruism overall, in a collective setting involving local fisheries resources the rate was higher, implying a greater willingness to incur personal cost. Ecological knowledge was high among experienced fishers, yet no knowledge pertaining to parallel management and or informal rules of exclusion or resource subtraction were observed, suggesting an informal management system did not exist. The study additionally documents freshwater biodiversity, providing an index of fish species present in the river collected from the free-listing exercises, categorized into native and non-native as the latter can negatively affect trophic systems and ecosystem processes. , Thesis (MSocSci) -- Faculty of Humanities, Anthropology, 2022
- Full Text:
Exploring the entrepreneurial intention of students in one Higher Education Institution (HEI)
- Authors: Kenye-Duma, Ayanda Rachel
- Date: 2022-04-06
- Subjects: Entrepreneurship South Africa , Intention , Entrepreneurship Study and teaching (Higher) South Africa , Students Attitudes , Social entrepreneurship , Social capital (Sociology) , Unemployment South Africa , Entrepreneurship Development in Higher Education (EDHE)
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/284514 , vital:56070
- Description: This study was interpretive and qualitatively explored students' entrepreneurial intentions at Rhodes University. It was conducted to understand entrepreneurship among students. Understanding students' entrepreneurship is critical as entrepreneurship is touted as a reasonable response to alleviate the high unemployment in South Africa. The study explored how students became entrepreneurs (or how their entrepreneurship intentions were formed) and the factors that enhanced (enablers and barriers) and inhibited them. The significance of living with and after COVID-19, youth and graduate unemployment alleviation places entrepreneurship development by institutions of higher learning, governments and private sector in the centre of economic recovery. The establishment of the Entrepreneurship Development in Higher Education (EDHE) in 2016, highlights the commitment of the South African government, for Higher Education Institutions (HEI) to engage more in entrepreneurship and do more research to understand how students become entrepreneurs and unravel the challenges. Understanding students' entrepreneurship intentions can facilitate a supportive environment for students entrepreneurship. With “very little research about student entrepreneurship at this university” being the guiding research problem, the aim was to gain insight into the entrepreneurship intention of university students by exploring factors that influence students to become entrepreneurs. The study employed Ajzen’s Theory of Planned Behaviour to explore the entrepreneurial intentions of university students in terms of the underlying attitudes, subjective norms, and perceived behavioural control. The students who participated in the study fell into three categories of entrepreneurial intention: (i) Intending to start a business, (ii) starting a business, and (ii) those who were already operating a business. All the participants, irrespective of their stage of entrepreneurial intention, demonstrated a positive attitude and self-efficacy. All participants showed self-efficacy by identifying barriers and taking positive steps to overcome them. The main barriers were access to funding, capital, training, business opportunities with the university and lack of interaction with established entrepreneurs. The results demonstrated the close interplay of attitudes, social norms and perceived behavioural control in students’ entrepreneurial intentions. Social recognition of entrepreneurship, social capital, education and role models contributed to positive attitudes, and social norms influenced students’ entrepreneurial intentions. A supportive environment was found to be critical for nurturing entrepreneurship intentions. The study recommends a supportive climate cultivated by Rhodes University to promote entrepreneurship. The supportive environment must include the elevation of entrepreneurship in prominent offices of the university, strengthening entrepreneurship societies and interactions with established entrepreneurs, and engagement with the private sector to contribute and promote entrepreneurship among students. , Thesis (MBA) -- Faculty of Commerce, Rhodes Business School, 2022
- Full Text:
- Authors: Kenye-Duma, Ayanda Rachel
- Date: 2022-04-06
- Subjects: Entrepreneurship South Africa , Intention , Entrepreneurship Study and teaching (Higher) South Africa , Students Attitudes , Social entrepreneurship , Social capital (Sociology) , Unemployment South Africa , Entrepreneurship Development in Higher Education (EDHE)
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/284514 , vital:56070
- Description: This study was interpretive and qualitatively explored students' entrepreneurial intentions at Rhodes University. It was conducted to understand entrepreneurship among students. Understanding students' entrepreneurship is critical as entrepreneurship is touted as a reasonable response to alleviate the high unemployment in South Africa. The study explored how students became entrepreneurs (or how their entrepreneurship intentions were formed) and the factors that enhanced (enablers and barriers) and inhibited them. The significance of living with and after COVID-19, youth and graduate unemployment alleviation places entrepreneurship development by institutions of higher learning, governments and private sector in the centre of economic recovery. The establishment of the Entrepreneurship Development in Higher Education (EDHE) in 2016, highlights the commitment of the South African government, for Higher Education Institutions (HEI) to engage more in entrepreneurship and do more research to understand how students become entrepreneurs and unravel the challenges. Understanding students' entrepreneurship intentions can facilitate a supportive environment for students entrepreneurship. With “very little research about student entrepreneurship at this university” being the guiding research problem, the aim was to gain insight into the entrepreneurship intention of university students by exploring factors that influence students to become entrepreneurs. The study employed Ajzen’s Theory of Planned Behaviour to explore the entrepreneurial intentions of university students in terms of the underlying attitudes, subjective norms, and perceived behavioural control. The students who participated in the study fell into three categories of entrepreneurial intention: (i) Intending to start a business, (ii) starting a business, and (ii) those who were already operating a business. All the participants, irrespective of their stage of entrepreneurial intention, demonstrated a positive attitude and self-efficacy. All participants showed self-efficacy by identifying barriers and taking positive steps to overcome them. The main barriers were access to funding, capital, training, business opportunities with the university and lack of interaction with established entrepreneurs. The results demonstrated the close interplay of attitudes, social norms and perceived behavioural control in students’ entrepreneurial intentions. Social recognition of entrepreneurship, social capital, education and role models contributed to positive attitudes, and social norms influenced students’ entrepreneurial intentions. A supportive environment was found to be critical for nurturing entrepreneurship intentions. The study recommends a supportive climate cultivated by Rhodes University to promote entrepreneurship. The supportive environment must include the elevation of entrepreneurship in prominent offices of the university, strengthening entrepreneurship societies and interactions with established entrepreneurs, and engagement with the private sector to contribute and promote entrepreneurship among students. , Thesis (MBA) -- Faculty of Commerce, Rhodes Business School, 2022
- Full Text:
Investigating the expression of three small open reading frames encoded on Helicoverpa armigera stunt virus RNA 1
- Authors: De Bruyn, Mart-Mari
- Date: 2017
- Subjects: Helicoverpa armigera , RNA viruses , Insects Viruses , Proteins
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/59168 , vital:27448
- Description: The Helicoverpa armigera stunt virus (HaSV), belonging to the Family Alphatetraviridae (Genus: Omegatetravirus), is a non-enveloped insect virus encapsidating a bi-partite, positive-sense single-stranded RNA genome. RNA1 encodes the replicase, as well as three small open reading frames (ORFs) arranged in tandem, and overlapping with the 3’ end of the replicase ORF. These ORFs, designated p11, p15 and p8, encode putative proteins of unknown function. The p11 and p15 ORFs are conserved in the genome of the related Omegatetravirus, Dendrolimus punctatus tetravirus. In HaSV, the stop codon of p11 is followed immediately by the start of p15, whereas the stop of p15 and start of p8 are separated by a glycine intercodon. Furthermore, only p11 is known to have a recognizable Kozak sequence. The aim of this study was to determine the expression and function of these three small proteins in the HaSV infectious lifecycle. The authenticity of the viral cDNA sequence, encoding the three small ORFs, was validated by sequencing multiple cDNA clones of the relevant region in viral RNA (vRNA), purified from infectious HaSV particles. The sequence of all three ORFs was conserved in seven cDNA clones, while point mutations were observed in each of two remaining cDNA clones, suggesting that the ORFs were conserved in infectious virus. Polyclonal antisera were raised against a p11 peptide, and a recombinant p15-p8 fusion protein (p23) expressed and purified from Escherichia coli. The affinity of the anti-p23 antiserum was confirmed by western blot analysis, while that of the anti-p11 antiserum was confirmed using immunofluorescence microscopy, as attempted expression of recombinant p11 in E. coli appeared to be toxic. The antisera were used to detect expression of the small proteins in HaSV-infected H. armigera larvae by western blot analysis. A band migrating at approximately 34 kDa was detected by both antisera in infected larvae, absent in uninfected larvae, suggesting the expression of a p11-p15-p8 polyprotein. Protein bands of 11 kDa and 8 kDa were also detected by the anti-p11 and anti-p23 antisera, respectively. Bioinformatic analysis revealed that the polyprotein would be produced by a novel type of stop codon read-through, however the mechanism required for individual expression could not be definitively determined. The mechanism by which these ORFs are translated was further investigated by expressing p11-p15, tagged with FLAG and enhanced green flourescent protein (EGFP) at its amino- and carboxyl-termini respectively (FLAG-p11-p15-EGFP), in Spodoptera frugiperda (Sf9) cells detected by flourescence microscopy. Punctate structures were observed throughout the cytoplasm that were also detected with antiFLAG, anti-p11 and anti-p23 antisera, complementing results obtained in previous studies. Since p15 does not exhibit a strong recognizable Kozak like p11, the dependency of p15 expression on that of p11 was investigated by mutating this construct such that p15 occurred in a +1 frame to p11. Both EGFP and anti-p23 fluorescence was detected with the same cytoplasmic distribution as the unmutated construct, whereas nothing was detected by anti-FLAG and anti-p11. Preliminary results therefore suggested p15 may also be expressed as a discrete protein, independent of p11. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2018
- Full Text:
- Authors: De Bruyn, Mart-Mari
- Date: 2017
- Subjects: Helicoverpa armigera , RNA viruses , Insects Viruses , Proteins
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/59168 , vital:27448
- Description: The Helicoverpa armigera stunt virus (HaSV), belonging to the Family Alphatetraviridae (Genus: Omegatetravirus), is a non-enveloped insect virus encapsidating a bi-partite, positive-sense single-stranded RNA genome. RNA1 encodes the replicase, as well as three small open reading frames (ORFs) arranged in tandem, and overlapping with the 3’ end of the replicase ORF. These ORFs, designated p11, p15 and p8, encode putative proteins of unknown function. The p11 and p15 ORFs are conserved in the genome of the related Omegatetravirus, Dendrolimus punctatus tetravirus. In HaSV, the stop codon of p11 is followed immediately by the start of p15, whereas the stop of p15 and start of p8 are separated by a glycine intercodon. Furthermore, only p11 is known to have a recognizable Kozak sequence. The aim of this study was to determine the expression and function of these three small proteins in the HaSV infectious lifecycle. The authenticity of the viral cDNA sequence, encoding the three small ORFs, was validated by sequencing multiple cDNA clones of the relevant region in viral RNA (vRNA), purified from infectious HaSV particles. The sequence of all three ORFs was conserved in seven cDNA clones, while point mutations were observed in each of two remaining cDNA clones, suggesting that the ORFs were conserved in infectious virus. Polyclonal antisera were raised against a p11 peptide, and a recombinant p15-p8 fusion protein (p23) expressed and purified from Escherichia coli. The affinity of the anti-p23 antiserum was confirmed by western blot analysis, while that of the anti-p11 antiserum was confirmed using immunofluorescence microscopy, as attempted expression of recombinant p11 in E. coli appeared to be toxic. The antisera were used to detect expression of the small proteins in HaSV-infected H. armigera larvae by western blot analysis. A band migrating at approximately 34 kDa was detected by both antisera in infected larvae, absent in uninfected larvae, suggesting the expression of a p11-p15-p8 polyprotein. Protein bands of 11 kDa and 8 kDa were also detected by the anti-p11 and anti-p23 antisera, respectively. Bioinformatic analysis revealed that the polyprotein would be produced by a novel type of stop codon read-through, however the mechanism required for individual expression could not be definitively determined. The mechanism by which these ORFs are translated was further investigated by expressing p11-p15, tagged with FLAG and enhanced green flourescent protein (EGFP) at its amino- and carboxyl-termini respectively (FLAG-p11-p15-EGFP), in Spodoptera frugiperda (Sf9) cells detected by flourescence microscopy. Punctate structures were observed throughout the cytoplasm that were also detected with antiFLAG, anti-p11 and anti-p23 antisera, complementing results obtained in previous studies. Since p15 does not exhibit a strong recognizable Kozak like p11, the dependency of p15 expression on that of p11 was investigated by mutating this construct such that p15 occurred in a +1 frame to p11. Both EGFP and anti-p23 fluorescence was detected with the same cytoplasmic distribution as the unmutated construct, whereas nothing was detected by anti-FLAG and anti-p11. Preliminary results therefore suggested p15 may also be expressed as a discrete protein, independent of p11. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2018
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