Exploring social learning practices for catchment management: a case study of two catchments in South Africa
- Authors: Siyengo, Kwanele
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/464848 , vital:76550
- Description: Social learning is achieved through a wide range of practices and is understood in different ways through multiple definitions in the literature. It is recognised as an outcome of stakeholder engagement through collaborative activities such as catchment management. Stakeholder engagement and collaboration in catchment management helps stakeholders understand the complex systems they work in, by enabling deliberation, dialogue, knowledge sharing and interdependencies. These are seen as processes which enable not only the sustainable use of natural resources but also help to achieve outcomes of transformative social learning. In partnership with the Living Catchments Project (implemented by the South African National Biodiversity Institute - SANBI), this study explores social learning practices and facilitation – and the role these play for transformation. Exploring two case studies, the Olifants River catchment and the Umzimvubu River catchment, the study adopted a qualitative participatory case study approach. It used observations, semi-structured interviews and reflections to look at the social learning practices in the two catchments. Additionally, it made use of the Social Learning, Knowledge Management and Mediation (SLKMM) framework as an analytical tool to investigate social learning practices and tools in the cases and explore how support for these can be enhanced in future. The results showed that there are existing social learning platforms and facilitation practices in the two catchments. Though not often explicit, social learning exists, and various tools (analytical, visual, participatory, and conceptual) and practices can be used to facilitate social learning. The greatest challenge for transformative social learning in these catchments is the need for a monitoring and evaluation practice which documents and makes explicit important learning and transformation taking place. This is therefore why, through a set of recommendations, it is suggested that it is key to build capacities within existing facilitators and to work closely with stakeholders from research, policy, and implementation to grow the existing social learning work, for future transformation. , Thesis (MSc) -- Faculty of Science, Environmental Science, 2024
- Full Text:
- Date Issued: 2024-10-11
- Authors: Siyengo, Kwanele
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/464848 , vital:76550
- Description: Social learning is achieved through a wide range of practices and is understood in different ways through multiple definitions in the literature. It is recognised as an outcome of stakeholder engagement through collaborative activities such as catchment management. Stakeholder engagement and collaboration in catchment management helps stakeholders understand the complex systems they work in, by enabling deliberation, dialogue, knowledge sharing and interdependencies. These are seen as processes which enable not only the sustainable use of natural resources but also help to achieve outcomes of transformative social learning. In partnership with the Living Catchments Project (implemented by the South African National Biodiversity Institute - SANBI), this study explores social learning practices and facilitation – and the role these play for transformation. Exploring two case studies, the Olifants River catchment and the Umzimvubu River catchment, the study adopted a qualitative participatory case study approach. It used observations, semi-structured interviews and reflections to look at the social learning practices in the two catchments. Additionally, it made use of the Social Learning, Knowledge Management and Mediation (SLKMM) framework as an analytical tool to investigate social learning practices and tools in the cases and explore how support for these can be enhanced in future. The results showed that there are existing social learning platforms and facilitation practices in the two catchments. Though not often explicit, social learning exists, and various tools (analytical, visual, participatory, and conceptual) and practices can be used to facilitate social learning. The greatest challenge for transformative social learning in these catchments is the need for a monitoring and evaluation practice which documents and makes explicit important learning and transformation taking place. This is therefore why, through a set of recommendations, it is suggested that it is key to build capacities within existing facilitators and to work closely with stakeholders from research, policy, and implementation to grow the existing social learning work, for future transformation. , Thesis (MSc) -- Faculty of Science, Environmental Science, 2024
- Full Text:
- Date Issued: 2024-10-11
Thermoluminescence and phototransferred thermoluminescence of tanzanite
- Authors: Opoku, Kingsley Acheampong
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/464914 , vital:76556
- Description: The thermoluminescence (TL) and phototransferred thermoluminescence (PTTL) properties of tanzanite, an extremely rare gem mineral, have been investigated. While tanzanite shows sensitivity to thermal and optical stimulation of luminescence techniques used for defect probing in insulators, it has received little attention in this regard. A glow curve corresponding to 70 Gy and measured at 1 °C s-1 revealed a high intensity peak at 74 °C (peak I) and two secondary peaks at 138 and 186 °C (peaks II and III). All the peaks exhibit a first order kinetics characteristics, as their positions remained unaffected by changes in either dose or partial heating (𝑇𝑚 − 𝑇𝑠𝑡𝑜𝑝). For variable doses from 10 to 200 Gy, the dose response of each peak is sublinear from the analysis of supralinearity indices. Peak I fades at room temperature when readout is delayed following irradiation, and this loss is due to thermal fading. The secondary peaks do not fade. Various methods of kinetic analysis were used to compute the kinetic parameters. For the respective peaks, the activation energy is about 0.84, 1.00 and 1.19 eV. All the peaks suffer thermal quenching with increasing heating rate. Continuous wave optically stimulated luminescence measurements were conducted to supplement the TL analysis with the aim of evaluating the kinetic parameters activation energy of thermal assistance (𝐸𝑎) and quenching (𝛥𝐸). The OSL source traps are the same as the TL source traps and occur within 40 to 90 °C, 110 to 145 °C and 160 to 220 °C. The kinetic parameter 𝛥𝐸 when luminescence from all the source traps is considered is comparable to that when source traps within 110 to 220 °C are measured and does not depend on the dose (10, 50 or 70Gy). Except for irradiation to 10 Gy, 𝐸𝑎 is also comparable. For traps within 160 to 220 °C, 𝛥𝐸 is comparable for the different doses but higher than that of the other traps, and a similar pattern was observed for 𝐸𝑎. Measurement of the PTTL signal induced by 470 nm blue LEDs following irradiation to 150 Gy and preheating to 158 °C showed that the TL peaks I and II were reproduced under phototransfer. The analysis for order of kinetics and dose response yielded the same results as the convention TL peaks. The model used to describe the PTTL intensity time response profiles shows that the PTTL emanates from a system of one acceptor and three donors, where the latter is a conglomerate of an unknown number of peaks. , Thesis (MSc) -- Faculty of Science, Physics and Electronics, 2024
- Full Text:
- Date Issued: 2024-10-11
- Authors: Opoku, Kingsley Acheampong
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/464914 , vital:76556
- Description: The thermoluminescence (TL) and phototransferred thermoluminescence (PTTL) properties of tanzanite, an extremely rare gem mineral, have been investigated. While tanzanite shows sensitivity to thermal and optical stimulation of luminescence techniques used for defect probing in insulators, it has received little attention in this regard. A glow curve corresponding to 70 Gy and measured at 1 °C s-1 revealed a high intensity peak at 74 °C (peak I) and two secondary peaks at 138 and 186 °C (peaks II and III). All the peaks exhibit a first order kinetics characteristics, as their positions remained unaffected by changes in either dose or partial heating (𝑇𝑚 − 𝑇𝑠𝑡𝑜𝑝). For variable doses from 10 to 200 Gy, the dose response of each peak is sublinear from the analysis of supralinearity indices. Peak I fades at room temperature when readout is delayed following irradiation, and this loss is due to thermal fading. The secondary peaks do not fade. Various methods of kinetic analysis were used to compute the kinetic parameters. For the respective peaks, the activation energy is about 0.84, 1.00 and 1.19 eV. All the peaks suffer thermal quenching with increasing heating rate. Continuous wave optically stimulated luminescence measurements were conducted to supplement the TL analysis with the aim of evaluating the kinetic parameters activation energy of thermal assistance (𝐸𝑎) and quenching (𝛥𝐸). The OSL source traps are the same as the TL source traps and occur within 40 to 90 °C, 110 to 145 °C and 160 to 220 °C. The kinetic parameter 𝛥𝐸 when luminescence from all the source traps is considered is comparable to that when source traps within 110 to 220 °C are measured and does not depend on the dose (10, 50 or 70Gy). Except for irradiation to 10 Gy, 𝐸𝑎 is also comparable. For traps within 160 to 220 °C, 𝛥𝐸 is comparable for the different doses but higher than that of the other traps, and a similar pattern was observed for 𝐸𝑎. Measurement of the PTTL signal induced by 470 nm blue LEDs following irradiation to 150 Gy and preheating to 158 °C showed that the TL peaks I and II were reproduced under phototransfer. The analysis for order of kinetics and dose response yielded the same results as the convention TL peaks. The model used to describe the PTTL intensity time response profiles shows that the PTTL emanates from a system of one acceptor and three donors, where the latter is a conglomerate of an unknown number of peaks. , Thesis (MSc) -- Faculty of Science, Physics and Electronics, 2024
- Full Text:
- Date Issued: 2024-10-11
Distribution and habitat preferences of marine megafauna in Nosy Be, Madagascar
- Authors: Lubbé, Alicea
- Date: 2023-10-13
- Subjects: Marine animals Habitat Madagascar Nosy Be , Marine animals Geographical distribution Madagascar Nosy Be , Species distribution modelling , Maximum entropy method , Citizen science , Habitat preference
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424710 , vital:72177
- Description: Marine megafauna have important ecological roles including the top-down regulation of lower trophic levels and the transport of nutrients. They are also charismatic species of socioeconomic importance, due to their public appeal and focus for ecotourism. However, these taxa face numerous anthropogenic threats including bycatch, habitat-loss, noise disturbance, prey reductions, pollution, and vessel traffic. Northwest Madagascar has been identified as an important marine mammal area and whale shark aggregation site, yet limited information on megafauna within Madagascar exists. In this thesis, I investigated the community structure, distribution trends, related environmental factors (Chapter 2) and predicted habitat suitability (Chapter 3) using a species distribution modelling approach for marine megafauna around Nosy Be, North-west Madagascar. Data collection consisted of opportunistic boatbased surveys conducted while looking for whale sharks, mobulid rays and cetaceans. These activities were performed by dedicated ‘swim with whale shark’ tourism operators, Les Baleines Rand'eau and Safari Baleine during tourism activities directed by the Madagascar Whale Shark Project over four years (2016-2019). A total of 1792 sightings were reported with 13 identified species, including elasmobranchs: whale shark, Rhincodon typus; spinetail mobula ray, Mobula japonica; giant oceanic manta ray, Manta birostris; shortfin devil ray, Mobula kuhlii; balaenids: humpback whale, Megaptera novaeangliae; Omura's whale, Balaenoptera omurai, delphinids: Indo-Pacific bottlenose dolphin, Tursiops aduncus; spinner dolphin, Stenella longirostris; pantropical spotted dolphin, Stenella attenuata; Indian ocean humpback dolphin, Sousa plumbea; false killer whale, Pseudorca crassidens; melonheaded whale, Peponocephala electra and one cheloniid species: leatherback sea turtle, Dermochelys coriacea. Sightings were spatially analysed at a community level according to feeding guilds (i.e., filter feeders vs. predators), and distribution of the megafauna groups v were compared to selected remotely sensed physico-chemical data (sea surface temperature and chlorophyll-a concentration) and bathymetry charts (depth and slope) using generalized linear models (Chapter 2). All four variables had a significant effect on filter feeder distribution. Spatial distribution of filter feeder sightings peaked at an area of steep underwater topography (18-50m; x slope=0.5%) located on the west coast of Nosy Be, where foraging aggregations of large filter feeders (primarily R. typus and B. omurai) were frequently observed. Only SST had a significant effect on the relative abundance of animals in the predator feeding guild, which demonstrated a more longitudinal distribution along the continental shelf. The maximum entropy model (Maxent) was used for predicting habitat preference for the most frequently sighted species (Chapter 3). The distribution of whale sharks, mobulid rays and Omura’s whales were significantly overlapped as they were abundant in two main hotspots, Grand banc de l’entrée (~10 km of the west coast) and near Nosy Mitsio island, (~50 km northeast of Nosy Be). Suitable habitat for the dolphins was in relatively shallow waters in temperatures <28ºC near areas of steep changes in bathymetry. Bottlenose and spinner dolphins had a more widespread distribution across the continental shelf and humpback dolphin was closely associated with inshore reefs and occur in waters <10m. The coastal and inshore region of Nosy Be is an important habitat for a variety of megafaunal species, and the continental shelf provided essential areas for feeding and breeding. The results were similar to those presented for these species in other regions of the Indian Ocean (i.e., Eastern and Southern Africa; Western Australia; Seychelles) as well as outside of the Indian Ocean (i.e., Indonesia; Caribbean; North Atlantic). Thus the results from this thesis could be considered for management recommendations for marine conservation in the region. The value and limitations of data collected by citizen science were outlined (Chapter 4). , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Lubbé, Alicea
- Date: 2023-10-13
- Subjects: Marine animals Habitat Madagascar Nosy Be , Marine animals Geographical distribution Madagascar Nosy Be , Species distribution modelling , Maximum entropy method , Citizen science , Habitat preference
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424710 , vital:72177
- Description: Marine megafauna have important ecological roles including the top-down regulation of lower trophic levels and the transport of nutrients. They are also charismatic species of socioeconomic importance, due to their public appeal and focus for ecotourism. However, these taxa face numerous anthropogenic threats including bycatch, habitat-loss, noise disturbance, prey reductions, pollution, and vessel traffic. Northwest Madagascar has been identified as an important marine mammal area and whale shark aggregation site, yet limited information on megafauna within Madagascar exists. In this thesis, I investigated the community structure, distribution trends, related environmental factors (Chapter 2) and predicted habitat suitability (Chapter 3) using a species distribution modelling approach for marine megafauna around Nosy Be, North-west Madagascar. Data collection consisted of opportunistic boatbased surveys conducted while looking for whale sharks, mobulid rays and cetaceans. These activities were performed by dedicated ‘swim with whale shark’ tourism operators, Les Baleines Rand'eau and Safari Baleine during tourism activities directed by the Madagascar Whale Shark Project over four years (2016-2019). A total of 1792 sightings were reported with 13 identified species, including elasmobranchs: whale shark, Rhincodon typus; spinetail mobula ray, Mobula japonica; giant oceanic manta ray, Manta birostris; shortfin devil ray, Mobula kuhlii; balaenids: humpback whale, Megaptera novaeangliae; Omura's whale, Balaenoptera omurai, delphinids: Indo-Pacific bottlenose dolphin, Tursiops aduncus; spinner dolphin, Stenella longirostris; pantropical spotted dolphin, Stenella attenuata; Indian ocean humpback dolphin, Sousa plumbea; false killer whale, Pseudorca crassidens; melonheaded whale, Peponocephala electra and one cheloniid species: leatherback sea turtle, Dermochelys coriacea. Sightings were spatially analysed at a community level according to feeding guilds (i.e., filter feeders vs. predators), and distribution of the megafauna groups v were compared to selected remotely sensed physico-chemical data (sea surface temperature and chlorophyll-a concentration) and bathymetry charts (depth and slope) using generalized linear models (Chapter 2). All four variables had a significant effect on filter feeder distribution. Spatial distribution of filter feeder sightings peaked at an area of steep underwater topography (18-50m; x slope=0.5%) located on the west coast of Nosy Be, where foraging aggregations of large filter feeders (primarily R. typus and B. omurai) were frequently observed. Only SST had a significant effect on the relative abundance of animals in the predator feeding guild, which demonstrated a more longitudinal distribution along the continental shelf. The maximum entropy model (Maxent) was used for predicting habitat preference for the most frequently sighted species (Chapter 3). The distribution of whale sharks, mobulid rays and Omura’s whales were significantly overlapped as they were abundant in two main hotspots, Grand banc de l’entrée (~10 km of the west coast) and near Nosy Mitsio island, (~50 km northeast of Nosy Be). Suitable habitat for the dolphins was in relatively shallow waters in temperatures <28ºC near areas of steep changes in bathymetry. Bottlenose and spinner dolphins had a more widespread distribution across the continental shelf and humpback dolphin was closely associated with inshore reefs and occur in waters <10m. The coastal and inshore region of Nosy Be is an important habitat for a variety of megafaunal species, and the continental shelf provided essential areas for feeding and breeding. The results were similar to those presented for these species in other regions of the Indian Ocean (i.e., Eastern and Southern Africa; Western Australia; Seychelles) as well as outside of the Indian Ocean (i.e., Indonesia; Caribbean; North Atlantic). Thus the results from this thesis could be considered for management recommendations for marine conservation in the region. The value and limitations of data collected by citizen science were outlined (Chapter 4). , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
Evaluating the potential effects of microplastics at environmentally realistic concentrations in South African freshwater systems
- Authors: Mtintsilana, Zintle
- Date: 2023-10-13
- Subjects: Microplastics , Water Pollution , Aquatic organisms Effect of water pollution on
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424160 , vital:72129
- Description: Microplastic pollution is spatially broad, microplastics can be found in various habitats including freshwater systems. Microplastic exposure to aquatic organisms has been associated with several physical impacts on aquatic organisms from multiple trophic levels such as; increased immune response, a decrease in food intake, excessive loss of weight, reduced growth rate, reduced energy and adverse effects on successive generations. However, these significant effects of microplastics exposure have been observed mostly in studies that use concentrations of microplastics that exceed environmental concentrations. Therefore, there is an overall lack of research on the effects of microplastics on freshwater organisms using environmentally realistic concentrations, especially in the Southern Hemisphere. A series of toxicity tests were used to expose a range of taxa including Tilapia sparrmanii, Caridina nilotica, and Melanoides tuberculata to different environmentally realistic concentrations of microplastics of different polymers. The study results show that the environmentally realistic concentrations had no statistically significant effects on most of the chosen test organisms and selected study endpoints, except for T. sparrmanii microplastic particle egestion and growth in polyethylene exposures which produced significant results. Although this study showed that at environmentally realistic concentrations and 21 days of exposure, minute effects on the test taxa were detected, various studies have proven that with longer exposure to microplastics, significant effects on freshwater organisms can be detected. Additionally, studies using concentrations higher than the current environmental concentrations have recorded significant effects on organisms and therefore, with increasing concentrations in the environment, more significant effects may be observed. Therefore, plastic pollution in the environment should be reduced as microplastics are in continuous production and circulation, and microplastic concentrations in freshwater environments are predicted to increase. , Thesis (MSc) -- Faculty of Science, Institute for Water Research, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Mtintsilana, Zintle
- Date: 2023-10-13
- Subjects: Microplastics , Water Pollution , Aquatic organisms Effect of water pollution on
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424160 , vital:72129
- Description: Microplastic pollution is spatially broad, microplastics can be found in various habitats including freshwater systems. Microplastic exposure to aquatic organisms has been associated with several physical impacts on aquatic organisms from multiple trophic levels such as; increased immune response, a decrease in food intake, excessive loss of weight, reduced growth rate, reduced energy and adverse effects on successive generations. However, these significant effects of microplastics exposure have been observed mostly in studies that use concentrations of microplastics that exceed environmental concentrations. Therefore, there is an overall lack of research on the effects of microplastics on freshwater organisms using environmentally realistic concentrations, especially in the Southern Hemisphere. A series of toxicity tests were used to expose a range of taxa including Tilapia sparrmanii, Caridina nilotica, and Melanoides tuberculata to different environmentally realistic concentrations of microplastics of different polymers. The study results show that the environmentally realistic concentrations had no statistically significant effects on most of the chosen test organisms and selected study endpoints, except for T. sparrmanii microplastic particle egestion and growth in polyethylene exposures which produced significant results. Although this study showed that at environmentally realistic concentrations and 21 days of exposure, minute effects on the test taxa were detected, various studies have proven that with longer exposure to microplastics, significant effects on freshwater organisms can be detected. Additionally, studies using concentrations higher than the current environmental concentrations have recorded significant effects on organisms and therefore, with increasing concentrations in the environment, more significant effects may be observed. Therefore, plastic pollution in the environment should be reduced as microplastics are in continuous production and circulation, and microplastic concentrations in freshwater environments are predicted to increase. , Thesis (MSc) -- Faculty of Science, Institute for Water Research, 2023
- Full Text:
- Date Issued: 2023-10-13
Thermal physiology of juvenile red roman seabream, Chrysoblephus laticeps after long-term exposure to low pH conditions
- Authors: Allison, Caitlin
- Date: 2023-10-13
- Subjects: Climatic changes , Ocean acidification , Basal metabolism , Chrysoblephus laticeps , Thermal tolerance (Physiology) , Phenotypic plasticity , Fishes Climatic factors
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424323 , vital:72143
- Description: Climate change has caused a combination of effects on the physiology of fishes. Of particular concern are the effects of thermal variability and ocean acidification. Organismal energy budgets change throughout ontogeny and research into the metabolic scope during early life stages is particularly useful in identifying potential bottlenecks. The first part of this thesis aimed to assess the absolute aerobic scope (AAS, described as the difference between the maximum and standard metabolic rates) of individual juveniles from a protected population of the endemic, commercially important seabream, Chrysoblephus laticeps, across a range of ecologically relevant temperatures (T = 11, 14, 18, 22˚C) under present-day conditions (pH = 8.03, pCO2 ≈ 420 μatm) using intermittent flow respirometry. The second component sought to investigate how long-term exposure (from fertilisation to juvenile, ~100 days exposure) to high-pCO2/hypercapnic conditions (pH = 7.63, pCO2 ≈ 1400 μatm), would affect the AAS of juvenile C. laticeps over a range of temperatures. Lower pH conditions were predicted to cause a decrease in the AAS of treatment animals due to additional energetic costs of acid-base regulation. The findings of the first data chapter demonstrated that juvenile C. laticeps reared under current CO2 conditions are tolerant to a wide range of thermal conditions, and individuals with a broad aerobic scope will be the best suited to coping with enhanced thermal variability. In contrast to the expected outcomes of the second data chapter, juvenile C. laticeps reared under high pCO2 conditions displayed greater AAS at high and low temperatures when compared with specimens from high pH conditions. Whilst a high degree of individual phenotypic variation was observed in the metabolic response of both groups, this was reduced at the lower and upper extreme temperatures for high pH and low pH animals respectively. Notably, the variation in treatment animal’s SMR was significantly diminished across all temperatures tested, compared to only a localised reduction in the SMR of high pH animals at cold temperatures. This may be indicative of compensatory pathways affecting energy restructuring and thermally-governed physiological trade-offs under hypercapnia. Given these results, juvenile C. laticeps appear to be more resilient to ocean acidification than anticipated, potentially owing to intrapopulation metabolic phenotypic diversity. This is likely attributed to the parental lineage originating in the Tsitsikamma MPA, which is thought to boast greater phenotypic diversity as a consequence of the refuge that these conservation areas offer from exploitation. Owing to the restriction imposed by the availability of surviving, captive-reared juveniles, the sample size used in this study was relatively low. However, owing to the repeated-measures nature of this research the sample size was sufficient to offer suitable statistical power for the polynomial mixed model used in the analysis. Future research should incorporate both physiological and behavioural responses to multiple environmental stressors to better understand covariation between these two traits, and to detect any behavioural trade-offs that might arise through compensation. In addition, these trials should be repeated using offspring from outside of the MPA to compare whether the same level of resilience and metabolic phenotypic diversity would be present in an exploited population. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Allison, Caitlin
- Date: 2023-10-13
- Subjects: Climatic changes , Ocean acidification , Basal metabolism , Chrysoblephus laticeps , Thermal tolerance (Physiology) , Phenotypic plasticity , Fishes Climatic factors
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424323 , vital:72143
- Description: Climate change has caused a combination of effects on the physiology of fishes. Of particular concern are the effects of thermal variability and ocean acidification. Organismal energy budgets change throughout ontogeny and research into the metabolic scope during early life stages is particularly useful in identifying potential bottlenecks. The first part of this thesis aimed to assess the absolute aerobic scope (AAS, described as the difference between the maximum and standard metabolic rates) of individual juveniles from a protected population of the endemic, commercially important seabream, Chrysoblephus laticeps, across a range of ecologically relevant temperatures (T = 11, 14, 18, 22˚C) under present-day conditions (pH = 8.03, pCO2 ≈ 420 μatm) using intermittent flow respirometry. The second component sought to investigate how long-term exposure (from fertilisation to juvenile, ~100 days exposure) to high-pCO2/hypercapnic conditions (pH = 7.63, pCO2 ≈ 1400 μatm), would affect the AAS of juvenile C. laticeps over a range of temperatures. Lower pH conditions were predicted to cause a decrease in the AAS of treatment animals due to additional energetic costs of acid-base regulation. The findings of the first data chapter demonstrated that juvenile C. laticeps reared under current CO2 conditions are tolerant to a wide range of thermal conditions, and individuals with a broad aerobic scope will be the best suited to coping with enhanced thermal variability. In contrast to the expected outcomes of the second data chapter, juvenile C. laticeps reared under high pCO2 conditions displayed greater AAS at high and low temperatures when compared with specimens from high pH conditions. Whilst a high degree of individual phenotypic variation was observed in the metabolic response of both groups, this was reduced at the lower and upper extreme temperatures for high pH and low pH animals respectively. Notably, the variation in treatment animal’s SMR was significantly diminished across all temperatures tested, compared to only a localised reduction in the SMR of high pH animals at cold temperatures. This may be indicative of compensatory pathways affecting energy restructuring and thermally-governed physiological trade-offs under hypercapnia. Given these results, juvenile C. laticeps appear to be more resilient to ocean acidification than anticipated, potentially owing to intrapopulation metabolic phenotypic diversity. This is likely attributed to the parental lineage originating in the Tsitsikamma MPA, which is thought to boast greater phenotypic diversity as a consequence of the refuge that these conservation areas offer from exploitation. Owing to the restriction imposed by the availability of surviving, captive-reared juveniles, the sample size used in this study was relatively low. However, owing to the repeated-measures nature of this research the sample size was sufficient to offer suitable statistical power for the polynomial mixed model used in the analysis. Future research should incorporate both physiological and behavioural responses to multiple environmental stressors to better understand covariation between these two traits, and to detect any behavioural trade-offs that might arise through compensation. In addition, these trials should be repeated using offspring from outside of the MPA to compare whether the same level of resilience and metabolic phenotypic diversity would be present in an exploited population. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
Development of a continuous flow process towards the synthesis of fluvastatin: a lipid lowering drug
- Authors: Singata, Mzuvukile
- Date: 2022-12
- Subjects: Drug development – South Africa , Drugs --Law and legislation , Pharmaceutical technology -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59587 , vital:62175
- Description: Access to pharmaceuticals in low to middle income countries affects not only the health of a nation, but also the economy. Global pandemics such as the SARS COVID-19 pandemic have demonstrated this disparity. A potential solution to this crisis is an efficient and reliable means of local production of active pharmaceutical ingredients. Current manufacturing plants heavily rely on batch processes as their primary form of production. Batch production has been shown to have numerous disadvantages that impede the reliable production of drugs. Flow chemistry presents a possible solution to this impediment. Numerous advantages of flow chemistry make this technology a better and safer option for production; these include improved safety, faster reaction times and improved reaction control. In this research we present the use of flow chemistry towards the synthesis of fluvastatin, a lipid lowering drug for individuals that suffer from hypercholesterolemia, which is characterised by elevated levels of low-density lipoprotein that is sometimes referred to as bad cholesterol. Chapter 1 provides a detailed description of the background to the problem of hypercholesterolemia; in this chapter the role of cholesterol in the body and how it can end up becoming a problem is outlined. An in-depth analysis of flow chemistry is provided and finally the problem statement, aims and objectives are presented. Chapter 2 give a detailed description of the instrumentation and chemicals used in the research. A description of experimental methodology towards the synthesis of all intermediates in batch and flow systems is provided. Chapter 3 provides all the data collected from the research and describes a multivariate optimization of the intermediates towards the synthesis of fluvastatin. The first intermediate was successfully synthesized in batch (60%) and flow systems at an improved conversion of 98% in a residence time of 15 minutes. The second intermediate was also obtained in batch (32%) and using continuous flow systems at a conversion of 75% and a residence time of 25 minutes. The third intermediate was successfully cyclized in batch using ZnCl2 (50%) and via continuous flow using Amberlite IR120 in a packed column reactor at a residence time of 15 minutes at 95°C, to obtain a conversion of 98%. The final intermediate was successfully synthesized in batch (62%) and in flow; it was obtained at 98% at a residence time of 15 minutes using continuous flow. Chapter 4 provides a conclusion of the study; it also provides a description of the future work and recommendations for this research. , Thesis (MSc) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2022
- Full Text:
- Date Issued: 2022-12
Development of a continuous flow process towards the synthesis of fluvastatin: a lipid lowering drug
- Authors: Singata, Mzuvukile
- Date: 2022-12
- Subjects: Drug development – South Africa , Drugs --Law and legislation , Pharmaceutical technology -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59587 , vital:62175
- Description: Access to pharmaceuticals in low to middle income countries affects not only the health of a nation, but also the economy. Global pandemics such as the SARS COVID-19 pandemic have demonstrated this disparity. A potential solution to this crisis is an efficient and reliable means of local production of active pharmaceutical ingredients. Current manufacturing plants heavily rely on batch processes as their primary form of production. Batch production has been shown to have numerous disadvantages that impede the reliable production of drugs. Flow chemistry presents a possible solution to this impediment. Numerous advantages of flow chemistry make this technology a better and safer option for production; these include improved safety, faster reaction times and improved reaction control. In this research we present the use of flow chemistry towards the synthesis of fluvastatin, a lipid lowering drug for individuals that suffer from hypercholesterolemia, which is characterised by elevated levels of low-density lipoprotein that is sometimes referred to as bad cholesterol. Chapter 1 provides a detailed description of the background to the problem of hypercholesterolemia; in this chapter the role of cholesterol in the body and how it can end up becoming a problem is outlined. An in-depth analysis of flow chemistry is provided and finally the problem statement, aims and objectives are presented. Chapter 2 give a detailed description of the instrumentation and chemicals used in the research. A description of experimental methodology towards the synthesis of all intermediates in batch and flow systems is provided. Chapter 3 provides all the data collected from the research and describes a multivariate optimization of the intermediates towards the synthesis of fluvastatin. The first intermediate was successfully synthesized in batch (60%) and flow systems at an improved conversion of 98% in a residence time of 15 minutes. The second intermediate was also obtained in batch (32%) and using continuous flow systems at a conversion of 75% and a residence time of 25 minutes. The third intermediate was successfully cyclized in batch using ZnCl2 (50%) and via continuous flow using Amberlite IR120 in a packed column reactor at a residence time of 15 minutes at 95°C, to obtain a conversion of 98%. The final intermediate was successfully synthesized in batch (62%) and in flow; it was obtained at 98% at a residence time of 15 minutes using continuous flow. Chapter 4 provides a conclusion of the study; it also provides a description of the future work and recommendations for this research. , Thesis (MSc) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2022
- Full Text:
- Date Issued: 2022-12
An investigation into water and sanitation in the Eastern Cape Province and potential for implementation of biotechnology platforms
- Authors: Gumunyu, Wilbert
- Date: 2022-10-14
- Subjects: Sewage South Africa Eastern Cape , Sanitation South Africa Eastern Cape , Algae Biotechnology , Biotechnology , Sewage Purification Biological treatment , Algal biofuels
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362830 , vital:65366
- Description: In contemporary South Africa, a country in transition, destruction rather than reconstruction seems commonplace. Electricity supply is at an all-time low and ‘load shedding’ is an almost daily occurrence. Similarly, more fragility of water delivery and sanitation service is being reported with the likelihood of ‘water shedding’ a soon-to-be reality. In view of the ever-increasing reported mismanagement of South Africa’s water and sanitation infrastructure, which is likely nearing the point of collapse if not already collapsed, this thesis set out to interrogate at a provincial and municipal level the status of water and sanitation in Chris Hani District Municipality (CHDM), Eastern Cape Province. One major driver behind this study was to evaluate provincial and municipal water/wastewater infrastructure as a platform to support implementation of locally developed bioprocess technologies. First, publicly available historical data was used to derive a baseline or benchmark from which to determine gains/losses in compliance, water quality and efficiency. The period 2009-2013/4 during which Blue and Green Drop reporting was routine, was used as an appropriate start point. Data thus derived indicated that, at provincial and district municipality (DM) level, potable water supply was by bulk water schemes and, sanitation was typically by waste stabilization pond (WSP) treatment of municipal sewage. The derived benchmark for the period 2009-2013/14 indicated that most plants were not compliant (~75% of the Eastern Cape Province water treatment plants (WTP) operating between average performance to critical state), operated either at- or above design capacity (Eastern Cape Province, 16%; CHDM, 19%). A large number of plants for the province (62.4%) and district (81.25%) had hydraulic design capacity or average daily flows that were unknown (information not provided in available literature or plant reports) and final effluent did not always meet the general standard (70.3% of the CHDM wastewater treatment works (WWTW)). To determine the contemporary state (2020 - 2022) of water and sanitation within CHDM, a scoping exercise of WTP and WWTW in selected local municipalities was carried out. This was done along a west-east transect by appraising WTP and WWTW in the towns of Cradock, Tarkastad, Komani, Tsomo, Cacadu, and Ngcobo. Furthermore, targeted assessment of the Queenstown WTP and the Cradock WWTW was used to derive real-time data on the status of water and sanitation infrastructure. Results showed that water and sanitation services within CHDM had, in the years between 2009-2013/14 to date, deteriorated. Freshwater demand significantly exceeded capability of water supply schemes, where demand was as much as three times greater than reported available supply. For most WWTW, operation was in excess of capacity and between 1.52 and 12 times installed hydraulic loading. The targeted scoping exercise revealed that Queenstown WTP is a moderate risk plant (Cumulative risk rating, CRR = 11 and maximum risk rating, MRR = 47.8%), whereas Cradock WWTW was in working condition but with challenges and some infrastructural dysfunction. Assessment of water/effluent quality revealed that turbidity and TOC were above SANS 241 general limit. Non-compliance in terms of nitrate/nitrite-N, ammonium-N, phosphate-P, TSS, total coliforms, E. Coli, and free chlorine was evident at Cradock WWTW. Unstructured interviews with plant operators corroborated these findings. The overwhelming support for bulk schemes for potable water provision and WSP for sanitation indicated a partially water secure municipal district but derelict in terms of its sanitation services. The later, it was concluded, in particular provides an ideal opportunity for implementation of platform technologies to support bioprocesses for entrepreneurship, employment, economic benefit and to secure a closed circular economy for regional water and sanitation through valorisation of co-product streams. Among the co-product streams considered in this thesis are biomass, biogas, biofertilizers, biofuels and several high value chemical products. , Thesis (MSc) -- Faculty of Science, Institute for Environmental Biotechnology, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Gumunyu, Wilbert
- Date: 2022-10-14
- Subjects: Sewage South Africa Eastern Cape , Sanitation South Africa Eastern Cape , Algae Biotechnology , Biotechnology , Sewage Purification Biological treatment , Algal biofuels
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362830 , vital:65366
- Description: In contemporary South Africa, a country in transition, destruction rather than reconstruction seems commonplace. Electricity supply is at an all-time low and ‘load shedding’ is an almost daily occurrence. Similarly, more fragility of water delivery and sanitation service is being reported with the likelihood of ‘water shedding’ a soon-to-be reality. In view of the ever-increasing reported mismanagement of South Africa’s water and sanitation infrastructure, which is likely nearing the point of collapse if not already collapsed, this thesis set out to interrogate at a provincial and municipal level the status of water and sanitation in Chris Hani District Municipality (CHDM), Eastern Cape Province. One major driver behind this study was to evaluate provincial and municipal water/wastewater infrastructure as a platform to support implementation of locally developed bioprocess technologies. First, publicly available historical data was used to derive a baseline or benchmark from which to determine gains/losses in compliance, water quality and efficiency. The period 2009-2013/4 during which Blue and Green Drop reporting was routine, was used as an appropriate start point. Data thus derived indicated that, at provincial and district municipality (DM) level, potable water supply was by bulk water schemes and, sanitation was typically by waste stabilization pond (WSP) treatment of municipal sewage. The derived benchmark for the period 2009-2013/14 indicated that most plants were not compliant (~75% of the Eastern Cape Province water treatment plants (WTP) operating between average performance to critical state), operated either at- or above design capacity (Eastern Cape Province, 16%; CHDM, 19%). A large number of plants for the province (62.4%) and district (81.25%) had hydraulic design capacity or average daily flows that were unknown (information not provided in available literature or plant reports) and final effluent did not always meet the general standard (70.3% of the CHDM wastewater treatment works (WWTW)). To determine the contemporary state (2020 - 2022) of water and sanitation within CHDM, a scoping exercise of WTP and WWTW in selected local municipalities was carried out. This was done along a west-east transect by appraising WTP and WWTW in the towns of Cradock, Tarkastad, Komani, Tsomo, Cacadu, and Ngcobo. Furthermore, targeted assessment of the Queenstown WTP and the Cradock WWTW was used to derive real-time data on the status of water and sanitation infrastructure. Results showed that water and sanitation services within CHDM had, in the years between 2009-2013/14 to date, deteriorated. Freshwater demand significantly exceeded capability of water supply schemes, where demand was as much as three times greater than reported available supply. For most WWTW, operation was in excess of capacity and between 1.52 and 12 times installed hydraulic loading. The targeted scoping exercise revealed that Queenstown WTP is a moderate risk plant (Cumulative risk rating, CRR = 11 and maximum risk rating, MRR = 47.8%), whereas Cradock WWTW was in working condition but with challenges and some infrastructural dysfunction. Assessment of water/effluent quality revealed that turbidity and TOC were above SANS 241 general limit. Non-compliance in terms of nitrate/nitrite-N, ammonium-N, phosphate-P, TSS, total coliforms, E. Coli, and free chlorine was evident at Cradock WWTW. Unstructured interviews with plant operators corroborated these findings. The overwhelming support for bulk schemes for potable water provision and WSP for sanitation indicated a partially water secure municipal district but derelict in terms of its sanitation services. The later, it was concluded, in particular provides an ideal opportunity for implementation of platform technologies to support bioprocesses for entrepreneurship, employment, economic benefit and to secure a closed circular economy for regional water and sanitation through valorisation of co-product streams. Among the co-product streams considered in this thesis are biomass, biogas, biofertilizers, biofuels and several high value chemical products. , Thesis (MSc) -- Faculty of Science, Institute for Environmental Biotechnology, 2022
- Full Text:
- Date Issued: 2022-10-14
Pharmacists’ attitudes and perception of using pictograms as a communication tool in practice
- Authors: Okeyo, Sam Juma
- Date: 2022-04-06
- Subjects: Picture-writing South Africa , Communication in public health South Africa , Pharmacists South Africa Attitudes , Health literacy South Africa , Patient education South Africa , Structural equation modeling , Theory of planned behavior
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/290693 , vital:56775
- Description: Pictograms, when used in conjunction with verbal and written information, are known to be effective in improving comprehension and recall of medicines information and in supporting communication between healthcare professionals and patients. However, pictograms are seldom used in routine pharmacy practice, and little is known about pharmacists’ opinions of pictograms and their intention to possibly incorporate pictograms into routine practice. This study aimed to investigate pharmacists’ opinions relating to pictograms as a communication tool, and, by applying the Theory of Planned Behaviour (TPB), to explore their intention to use pictograms in pharmacy practice as well as the barriers to their use. The quantitative study design involved a two-phase approach. Phase 1 was a descriptive, cross-sectional online national survey of pharmacists. The 70-item, four-section survey was primarily based on the constructs of TPB, which included attitude, intention, subjective norm, and perceived behavioural control. The last survey section recruited pharmacists for a follow-up Phase 2 survey. Following a pilot study, the survey was emailed to all pharmacists registered with the South African Pharmacy Council. Descriptive statistics for survey items were generated. Pearson correlation investigated the correlation between participant characteristics, familiarity with pictograms and use of pictograms in practice. Structural Equation Modelling (SEM) determined if there was a significant relationship between attitude, subjective norm and perceived behavioural control with intention to use pictograms. A total of 426 pharmacists responded to the Phase 1 survey. Most pharmacists were familiar with the term ‘pictogram’; however, over three-quarters of pharmacists had never observed pictograms being routinely used in a pharmacy setting. When presented with pictograms designed for a low health literate population, most pharmacists thought the design and overall look of the pictograms would be easy for most patients in South Africa to understand (71.6 ± 24.0). Two-thirds of pharmacists (65.0 ± 30.6), felt that pictograms should be used for all patient populations. More than 85% of pharmacists agreed that pictograms should be used for dosage instructions, auxiliary or additional information, warnings, and storage instructions. However, fewer (58-68%) felt that indication, side effects and risk communication information should be accompanied by pictograms. Pharmacists demonstrated positive attitudes towards using pictograms in practice (mean = 4.2 ± 0.9; range: 1 - 5), while perceived behavioural control (mean = 3.0 ± 1.2; range: 1 - 5), subjective norm (mean = 3.8 ± 1.0; range 1 - 5) and intention (mean = 3.3 ± 1.0; range 1 - 5) were all neutral. Attitude (β = -0.25, p < 0.117), however, was not a significant predictor of intention while perceived behavioural control (β = -0.83, p < 0.000) presented with a significant negative correlation with intention. Subjective norm (β = 0.57, p <0.000) was the strongest predictor of intention. Scale reliability ranged from 0.770 to 0.865 for the TPB constructs. Phase 2 aimed to expand on, and further investigate Phase 1 findings relating to current and intended pharmacist behaviour and opinions concerning pictogram usage. As Phase 2 looked to investigate issues in greater depth, questions included open-ended response options. The survey link was emailed to all pharmacists who had voluntarily offered to participate in Phase 2. Frequency data for all questions were generated, and content analysis was undertaken for the free-response comments offered by pharmacists. A total of 35 pharmacists responded to the Phase 2 survey. Most pharmacists who routinely used pictograms initiated their use with support from pharmacists’ colleagues (8/12) and their supervisor/manager (6/7). Pharmacists who stated their intention to use pictograms anticipated receiving support from their pharmacists' colleagues (18/23) and manager/supervisor (15/23). All 12 pharmacists who were routinely using pictograms reported a positive effect on patient communication, with almost all having encountered no negative aspects of using pictograms (11/12). Almost all pharmacists intending to use pictograms could foresee benefits from their use (22/23). Misinterpretation of pictograms was a prevalent barrier common to both pharmacists routinely using pictograms and to those intending to use pictograms. Increased workload was regarded as a prevalent barrier only by pharmacists intending to use pictograms. This study was the first national study of pharmacists to investigate their opinion of pictograms and their use and to adopt a theoretical approach to consider pharmacist intention to use pictograms in routine pharmacy practice. Pharmacists generally expressed positive attitudes to pictograms but showed inadequate understanding of pictogram use. Pharmacists using pictograms reported the positive effect of pictograms on their patient communication, whereas those planning to use pictograms could foresee the benefits of using pictograms despite regarding increased workload as a barrier. As the strongest predictor of intention was subjective norm, this construct should therefore be targeted to motivate pharmacists to adopt the use of pictograms. , Thesis (MPharm) -- Faculty of Pharmacy, Pharmacy, 2022
- Full Text:
- Date Issued: 2022-04-06
- Authors: Okeyo, Sam Juma
- Date: 2022-04-06
- Subjects: Picture-writing South Africa , Communication in public health South Africa , Pharmacists South Africa Attitudes , Health literacy South Africa , Patient education South Africa , Structural equation modeling , Theory of planned behavior
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/290693 , vital:56775
- Description: Pictograms, when used in conjunction with verbal and written information, are known to be effective in improving comprehension and recall of medicines information and in supporting communication between healthcare professionals and patients. However, pictograms are seldom used in routine pharmacy practice, and little is known about pharmacists’ opinions of pictograms and their intention to possibly incorporate pictograms into routine practice. This study aimed to investigate pharmacists’ opinions relating to pictograms as a communication tool, and, by applying the Theory of Planned Behaviour (TPB), to explore their intention to use pictograms in pharmacy practice as well as the barriers to their use. The quantitative study design involved a two-phase approach. Phase 1 was a descriptive, cross-sectional online national survey of pharmacists. The 70-item, four-section survey was primarily based on the constructs of TPB, which included attitude, intention, subjective norm, and perceived behavioural control. The last survey section recruited pharmacists for a follow-up Phase 2 survey. Following a pilot study, the survey was emailed to all pharmacists registered with the South African Pharmacy Council. Descriptive statistics for survey items were generated. Pearson correlation investigated the correlation between participant characteristics, familiarity with pictograms and use of pictograms in practice. Structural Equation Modelling (SEM) determined if there was a significant relationship between attitude, subjective norm and perceived behavioural control with intention to use pictograms. A total of 426 pharmacists responded to the Phase 1 survey. Most pharmacists were familiar with the term ‘pictogram’; however, over three-quarters of pharmacists had never observed pictograms being routinely used in a pharmacy setting. When presented with pictograms designed for a low health literate population, most pharmacists thought the design and overall look of the pictograms would be easy for most patients in South Africa to understand (71.6 ± 24.0). Two-thirds of pharmacists (65.0 ± 30.6), felt that pictograms should be used for all patient populations. More than 85% of pharmacists agreed that pictograms should be used for dosage instructions, auxiliary or additional information, warnings, and storage instructions. However, fewer (58-68%) felt that indication, side effects and risk communication information should be accompanied by pictograms. Pharmacists demonstrated positive attitudes towards using pictograms in practice (mean = 4.2 ± 0.9; range: 1 - 5), while perceived behavioural control (mean = 3.0 ± 1.2; range: 1 - 5), subjective norm (mean = 3.8 ± 1.0; range 1 - 5) and intention (mean = 3.3 ± 1.0; range 1 - 5) were all neutral. Attitude (β = -0.25, p < 0.117), however, was not a significant predictor of intention while perceived behavioural control (β = -0.83, p < 0.000) presented with a significant negative correlation with intention. Subjective norm (β = 0.57, p <0.000) was the strongest predictor of intention. Scale reliability ranged from 0.770 to 0.865 for the TPB constructs. Phase 2 aimed to expand on, and further investigate Phase 1 findings relating to current and intended pharmacist behaviour and opinions concerning pictogram usage. As Phase 2 looked to investigate issues in greater depth, questions included open-ended response options. The survey link was emailed to all pharmacists who had voluntarily offered to participate in Phase 2. Frequency data for all questions were generated, and content analysis was undertaken for the free-response comments offered by pharmacists. A total of 35 pharmacists responded to the Phase 2 survey. Most pharmacists who routinely used pictograms initiated their use with support from pharmacists’ colleagues (8/12) and their supervisor/manager (6/7). Pharmacists who stated their intention to use pictograms anticipated receiving support from their pharmacists' colleagues (18/23) and manager/supervisor (15/23). All 12 pharmacists who were routinely using pictograms reported a positive effect on patient communication, with almost all having encountered no negative aspects of using pictograms (11/12). Almost all pharmacists intending to use pictograms could foresee benefits from their use (22/23). Misinterpretation of pictograms was a prevalent barrier common to both pharmacists routinely using pictograms and to those intending to use pictograms. Increased workload was regarded as a prevalent barrier only by pharmacists intending to use pictograms. This study was the first national study of pharmacists to investigate their opinion of pictograms and their use and to adopt a theoretical approach to consider pharmacist intention to use pictograms in routine pharmacy practice. Pharmacists generally expressed positive attitudes to pictograms but showed inadequate understanding of pictogram use. Pharmacists using pictograms reported the positive effect of pictograms on their patient communication, whereas those planning to use pictograms could foresee the benefits of using pictograms despite regarding increased workload as a barrier. As the strongest predictor of intention was subjective norm, this construct should therefore be targeted to motivate pharmacists to adopt the use of pictograms. , Thesis (MPharm) -- Faculty of Pharmacy, Pharmacy, 2022
- Full Text:
- Date Issued: 2022-04-06
“Un-silencing queer Nigeria”: Representations of queerness in contemporary Nigerian fiction
- Authors: Akram, Tahzeeb
- Date: 2021-10-29
- Subjects: Heterosexism in literature , Patriarchy in literature , Homophobia in literature , Sexual minorities in literature , Nigerian literature 21st century History and criticism , Queer theory Nigeria , Gender identity Law and legislation Nigeria , Gender identity Religious aspects , Sexual minorities Nigeria Social conditions , Nigeria. Same Sex Marriage (Prohibition) Act, 2013
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190668 , vital:45016
- Description: This thesis analyses contemporary queer Nigerian fiction, particularly queer representations regarding law, religion and culture in Nigeria’s heteropatriarchal and queerphobic society. I explore a number of authors’ use of different literary forms and platforms to promote and represent non-conforming genders and sexualities in queerphobic Nigeria. These narratives show queer people navigating the heteronormative society vis-à-vis marriage, family, intimacy, work, violence and rights activism. I draw on Western and African gender and queer theories for the concepts, definitions and critical terminologies used in this thesis. African queer theorists and activists are highlighted for their reclaiming queer history from among the early records in Africa as well as contemporary queer Nigerian literature and activism. Religion and queerness are crucial themes in Chinelo Okparanta’s same sex women’s novel, Under the Udala Trees. Using queer African Christian theology against Nigeria’s conservative socio-religious setting, I demonstrate that queerness is not a threat to Nigerian’s Christian faith, and that mutual coexistence of queer sexuality and Christianity advances queer rights in that society. Nnanna Ikpo’s Fimí Sílẹ̀ Forever: Heaven gave it to me’s problematises heteronormative masculinities and the manufacturing of heteropatriarchy and queer masculinities in Nigeria. I examine the protagonists who are both victims of and perpetrators in their queerphobic society. The socio-legal focus I employ examines the impact of the 2014 Same Sex Marriage Prohibition Act on Nigeria’s already marginalised and oppressed queer community. There are vast opportunities for queer Nigerian artists to create, publish and promote queer identities in the safe and enabling space of online platforms via physical distancing between the queer community and the queerphobic society. From the digitally published 14: An Anthology of Queer Art’s two volumes, five short stories are analysed regarding different forms of intimacies in queer men’s relationships. These queer contemporary fiction writers offer complex representations of queerness within Nigeria’s heteropatriarchal and queerphobic society that polices non-normative bodies through religion, culture and the law. Such literary texts, digital literary platforms and activism vitally provide queer Nigerians a progressive space to assert queer presence, voices lives and rights to educating and re-socialising their society towards humaneness. , Thesis (MA) -- Faculty of Humanities, Literary Studies in English, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Akram, Tahzeeb
- Date: 2021-10-29
- Subjects: Heterosexism in literature , Patriarchy in literature , Homophobia in literature , Sexual minorities in literature , Nigerian literature 21st century History and criticism , Queer theory Nigeria , Gender identity Law and legislation Nigeria , Gender identity Religious aspects , Sexual minorities Nigeria Social conditions , Nigeria. Same Sex Marriage (Prohibition) Act, 2013
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190668 , vital:45016
- Description: This thesis analyses contemporary queer Nigerian fiction, particularly queer representations regarding law, religion and culture in Nigeria’s heteropatriarchal and queerphobic society. I explore a number of authors’ use of different literary forms and platforms to promote and represent non-conforming genders and sexualities in queerphobic Nigeria. These narratives show queer people navigating the heteronormative society vis-à-vis marriage, family, intimacy, work, violence and rights activism. I draw on Western and African gender and queer theories for the concepts, definitions and critical terminologies used in this thesis. African queer theorists and activists are highlighted for their reclaiming queer history from among the early records in Africa as well as contemporary queer Nigerian literature and activism. Religion and queerness are crucial themes in Chinelo Okparanta’s same sex women’s novel, Under the Udala Trees. Using queer African Christian theology against Nigeria’s conservative socio-religious setting, I demonstrate that queerness is not a threat to Nigerian’s Christian faith, and that mutual coexistence of queer sexuality and Christianity advances queer rights in that society. Nnanna Ikpo’s Fimí Sílẹ̀ Forever: Heaven gave it to me’s problematises heteronormative masculinities and the manufacturing of heteropatriarchy and queer masculinities in Nigeria. I examine the protagonists who are both victims of and perpetrators in their queerphobic society. The socio-legal focus I employ examines the impact of the 2014 Same Sex Marriage Prohibition Act on Nigeria’s already marginalised and oppressed queer community. There are vast opportunities for queer Nigerian artists to create, publish and promote queer identities in the safe and enabling space of online platforms via physical distancing between the queer community and the queerphobic society. From the digitally published 14: An Anthology of Queer Art’s two volumes, five short stories are analysed regarding different forms of intimacies in queer men’s relationships. These queer contemporary fiction writers offer complex representations of queerness within Nigeria’s heteropatriarchal and queerphobic society that polices non-normative bodies through religion, culture and the law. Such literary texts, digital literary platforms and activism vitally provide queer Nigerians a progressive space to assert queer presence, voices lives and rights to educating and re-socialising their society towards humaneness. , Thesis (MA) -- Faculty of Humanities, Literary Studies in English, 2021
- Full Text:
- Date Issued: 2021-10-29
Orchid mycorrhizal and endophytic fungal diversity of three co-occurring terrestrial orchids in the large African genus Disa (Orchidaceae)
- Khambule, Nondumiso Venessia
- Authors: Khambule, Nondumiso Venessia
- Date: 2020
- Subjects: Orchids South Africa , Mycorrhizal fungi South Africa , Endomycorrhizas South Africa , Endophytes , Orchids Roots
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/163341 , vital:41030
- Description: Orchids (in the family Orchidaceous) are one of the richest plant families and approximately 500 species are found in South Africa. A number of orchid species are found on disturbed areas and many of the terrestrial species grow in poor soils with low mineral nutrient availability. Most orchid species are thought to be associated with mycorrhizal fungi for germination and mycorrhiza provides nutrients for the survival of adult plants. The aim of this study was to select Orchidaceous plant species and to isolate, identify and characterize the orchid endophytes and assess these isolates for potential antimicrobial and enzymatic activities Isa is the largest genus in South Africa and three Disa species co-occurring in a small geographical area were selected. These included Disa bracteata, D. cornuta and D. polygonoides which span three sections of the genus. Roots were stained to confirm the mycorrhizal status of the Disa species. Mycorrhizal pelotons structures were microscopically observed inside root cells. The presence of pelotons is indictive of mycorrhizal fungal interactions within the orchid roots and areas associated with the site of nutrient exchange between plant and fungus. The presence of pelotons, however, does not give n indication of the fungal species involved. The endophytes were successfully isolated in pure cultures on potato dextrose agar (PDA). All slow growing isolates were selected, and further molecular identification undertaken; DNA was extracted, and PCR amplified using internal transcribed spacer (ITS1F and ITS4) fungal primers. The amplified products were then sequenced and analysed by comparison to sequences in the GenBank database. Trichoderma, Penicillium, Metapochonia, Talaromyces, Oidiodendron Neopestalotiopsis, and Chaetomium were identified from these sequences. The presence of other fungal root endophytes was suspected despite the rigorous surface sterilization procedure used. The primers used to amplify the ITS region are the universal barcoding primers which are specific to fungi. ITS1F is one of the primers designed to amplify a broad range of fungi. DNA was extracted from orchid roots and amplicons were cloned into a pGEMT plasmid vector. Individual clones were sequenced and aligned with Mega software and compared to sequences in the GenBank and UNITE database. Based on percentage sequence identity, unidentified Tulasnella species, Tullasnela colaspora, and various Ascomycota endophytes were identified as contributing to the endophytic root fungal diversity of the selected Disa species. The Disa species investigated in this study were associated with several soil endophytes. D. bracteata, D. polygonoides were collected from the same site along the road verge which is regarded as being disturbed. Based on both culture – dependent and independent techniques employed Oidiodendron was found associated with both species. Antimicrobial activity was determined using a well diffusion method using extracts from the isolated fungi against the bacterial isolates Bacillus cereus, Staphylococcus aureus, Escherichia coli and Pseudomonas puptida. Most of the isolated fungi showed at least one potential inhibition effect against one of the bacterial isolates. The extracts that showed potential antimicrobial activity could be further screened to determine the compounds produced as secondary metabolites using techniques such as LC-MS Enzymatic activities of protease, cellulose and amylase were determined using solid media amended with milk protein, carboxymethylcellulose (CMC) and starch. The majority of fungal isolates tested positive with amylase and cellulose with only a few fungal isolates testing positive for protease activity. Broth cultures containing CMC and starch were shown to enhance biomass production in approximately 40 % of the fungal isolates. Degradation of the substrates is required in order to provide carbon to the fungus under test in order to optimize fungal growth as well as to gain insight into their ecological role. Enzyme activity was evident particularly when cellulose and starch were provided as substrates. All the fungal isolates tested grew on the amended medium, with 40% of the isolates preferring to utilize CMC and/or starch, indicating the ability of these fungi to utilize various resources for carbon acquisitions. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2020
- Full Text:
- Date Issued: 2020
- Authors: Khambule, Nondumiso Venessia
- Date: 2020
- Subjects: Orchids South Africa , Mycorrhizal fungi South Africa , Endomycorrhizas South Africa , Endophytes , Orchids Roots
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/163341 , vital:41030
- Description: Orchids (in the family Orchidaceous) are one of the richest plant families and approximately 500 species are found in South Africa. A number of orchid species are found on disturbed areas and many of the terrestrial species grow in poor soils with low mineral nutrient availability. Most orchid species are thought to be associated with mycorrhizal fungi for germination and mycorrhiza provides nutrients for the survival of adult plants. The aim of this study was to select Orchidaceous plant species and to isolate, identify and characterize the orchid endophytes and assess these isolates for potential antimicrobial and enzymatic activities Isa is the largest genus in South Africa and three Disa species co-occurring in a small geographical area were selected. These included Disa bracteata, D. cornuta and D. polygonoides which span three sections of the genus. Roots were stained to confirm the mycorrhizal status of the Disa species. Mycorrhizal pelotons structures were microscopically observed inside root cells. The presence of pelotons is indictive of mycorrhizal fungal interactions within the orchid roots and areas associated with the site of nutrient exchange between plant and fungus. The presence of pelotons, however, does not give n indication of the fungal species involved. The endophytes were successfully isolated in pure cultures on potato dextrose agar (PDA). All slow growing isolates were selected, and further molecular identification undertaken; DNA was extracted, and PCR amplified using internal transcribed spacer (ITS1F and ITS4) fungal primers. The amplified products were then sequenced and analysed by comparison to sequences in the GenBank database. Trichoderma, Penicillium, Metapochonia, Talaromyces, Oidiodendron Neopestalotiopsis, and Chaetomium were identified from these sequences. The presence of other fungal root endophytes was suspected despite the rigorous surface sterilization procedure used. The primers used to amplify the ITS region are the universal barcoding primers which are specific to fungi. ITS1F is one of the primers designed to amplify a broad range of fungi. DNA was extracted from orchid roots and amplicons were cloned into a pGEMT plasmid vector. Individual clones were sequenced and aligned with Mega software and compared to sequences in the GenBank and UNITE database. Based on percentage sequence identity, unidentified Tulasnella species, Tullasnela colaspora, and various Ascomycota endophytes were identified as contributing to the endophytic root fungal diversity of the selected Disa species. The Disa species investigated in this study were associated with several soil endophytes. D. bracteata, D. polygonoides were collected from the same site along the road verge which is regarded as being disturbed. Based on both culture – dependent and independent techniques employed Oidiodendron was found associated with both species. Antimicrobial activity was determined using a well diffusion method using extracts from the isolated fungi against the bacterial isolates Bacillus cereus, Staphylococcus aureus, Escherichia coli and Pseudomonas puptida. Most of the isolated fungi showed at least one potential inhibition effect against one of the bacterial isolates. The extracts that showed potential antimicrobial activity could be further screened to determine the compounds produced as secondary metabolites using techniques such as LC-MS Enzymatic activities of protease, cellulose and amylase were determined using solid media amended with milk protein, carboxymethylcellulose (CMC) and starch. The majority of fungal isolates tested positive with amylase and cellulose with only a few fungal isolates testing positive for protease activity. Broth cultures containing CMC and starch were shown to enhance biomass production in approximately 40 % of the fungal isolates. Degradation of the substrates is required in order to provide carbon to the fungus under test in order to optimize fungal growth as well as to gain insight into their ecological role. Enzyme activity was evident particularly when cellulose and starch were provided as substrates. All the fungal isolates tested grew on the amended medium, with 40% of the isolates preferring to utilize CMC and/or starch, indicating the ability of these fungi to utilize various resources for carbon acquisitions. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2020
- Full Text:
- Date Issued: 2020
Factors to improve data quality of electronic medical records
- Authors: Makeleni, Noloyiso Anele
- Date: 2019
- Subjects: Electronic records , Medical records -- Management , Medical records -- Data processing
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/19881 , vital:43618
- Description: Electronic Medical Record (EMR) systems have been identified as having the potential to improve health care and allow the health care sector to reap a number of benefits when implemented successfully. These benefits include enabling quick and easy access to patient files and also reducing the problem of misplaced or lost patient files. Such EMRs allow for patient records to be up to date, provided that health care practitioners capture standard and consistent data in the relevant fields. In Africa, there are only a few countries that have successfully implemented EMR systems due to social and technological challenges. Social factors include lack of computer skilled health workers, lack of adequate training, physician’s resistance to shift from using paper records to electronic records, either due to complex systems or the fear of being replaced by the systems. On the other hand, the technological factors include lack of Information Technology (IT) and clinical resources, lack of internet access, financial barriers to purchase the necessary technological hardware and implementation costs. A few South African health care institutions have implemented EMR systems, however, most of the public health care facilities still make use of a manual system to capture patient information. In the case where public health care facilities do have an EMR system implemented, there are problems with the consistency of the data that is captured. The inconsistency is caused by the different understandings that the health care professionals have regarding the importance of capturing the necessary information that is collected at various points in health care institutions, thus affecting data quality. For the successful implementation and use of EMR systems, everything within the health care organisation should be integrated. In other words, the steering committee and workgroup, the equipment, the product, the processes, the system and the facility design and construction should be incorporated to work together. The common problems identified in literature regarding data quality in EMRs include misspelled words, inconsistent word strings, inaccurate information entered on the record and incompleteness of the record. These problems lead to poor quality information, lack of accessibility of the record, poorly organised notes and inaccurate information about the patient. The South African strategy aims to implement a National Health Insurance (NHI) which will provide citizens with equitable access to health care. For the successful implementation of the NHI strategy, South African health care sectors should address the barriers which were identified and learn from other African countries that have successfully implemented EMR systems and had positive outcomes. Therefore, this study investigates how data quality can be improved on electronic medical records in public health care in South Africa? The qualitative research methodology approach was used for this study. Interviews were conducted with eight health care professionals at Klerksdorp, in the North West province to obtain data regarding the factors they would deem important for the improvement of data quality in EMRs. The Data Quality Framework (DQF) was applied in this study and six dimensions were identified as the factors to improve data quality. These dimensions include completeness, accuracy, consistency, conformity, timeliness, and integrity. From the analysis of the interview responses, it was discovered that there were, in fact, data quality issues experienced at the public health care facilities of South Africa. A need was identified for the use of data quality assessment tools and solutions to address the data quality issues or challenges that health care practitioners are faced with during their daily jobs. Seven barriers were also identified as having an impact on the successful implementation of EMRs at health care institutions. These barriers, together with the data quality issues, influence the successful use of EMRs and should not be overlooked. From these barriers the study developed seven Critical Success Factors which can be used by the National Department of Health to improve the quality of EMRs. , Thesis (MCom) -- Faculty of Management and Commerce, 2019
- Full Text:
- Date Issued: 2019
- Authors: Makeleni, Noloyiso Anele
- Date: 2019
- Subjects: Electronic records , Medical records -- Management , Medical records -- Data processing
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/19881 , vital:43618
- Description: Electronic Medical Record (EMR) systems have been identified as having the potential to improve health care and allow the health care sector to reap a number of benefits when implemented successfully. These benefits include enabling quick and easy access to patient files and also reducing the problem of misplaced or lost patient files. Such EMRs allow for patient records to be up to date, provided that health care practitioners capture standard and consistent data in the relevant fields. In Africa, there are only a few countries that have successfully implemented EMR systems due to social and technological challenges. Social factors include lack of computer skilled health workers, lack of adequate training, physician’s resistance to shift from using paper records to electronic records, either due to complex systems or the fear of being replaced by the systems. On the other hand, the technological factors include lack of Information Technology (IT) and clinical resources, lack of internet access, financial barriers to purchase the necessary technological hardware and implementation costs. A few South African health care institutions have implemented EMR systems, however, most of the public health care facilities still make use of a manual system to capture patient information. In the case where public health care facilities do have an EMR system implemented, there are problems with the consistency of the data that is captured. The inconsistency is caused by the different understandings that the health care professionals have regarding the importance of capturing the necessary information that is collected at various points in health care institutions, thus affecting data quality. For the successful implementation and use of EMR systems, everything within the health care organisation should be integrated. In other words, the steering committee and workgroup, the equipment, the product, the processes, the system and the facility design and construction should be incorporated to work together. The common problems identified in literature regarding data quality in EMRs include misspelled words, inconsistent word strings, inaccurate information entered on the record and incompleteness of the record. These problems lead to poor quality information, lack of accessibility of the record, poorly organised notes and inaccurate information about the patient. The South African strategy aims to implement a National Health Insurance (NHI) which will provide citizens with equitable access to health care. For the successful implementation of the NHI strategy, South African health care sectors should address the barriers which were identified and learn from other African countries that have successfully implemented EMR systems and had positive outcomes. Therefore, this study investigates how data quality can be improved on electronic medical records in public health care in South Africa? The qualitative research methodology approach was used for this study. Interviews were conducted with eight health care professionals at Klerksdorp, in the North West province to obtain data regarding the factors they would deem important for the improvement of data quality in EMRs. The Data Quality Framework (DQF) was applied in this study and six dimensions were identified as the factors to improve data quality. These dimensions include completeness, accuracy, consistency, conformity, timeliness, and integrity. From the analysis of the interview responses, it was discovered that there were, in fact, data quality issues experienced at the public health care facilities of South Africa. A need was identified for the use of data quality assessment tools and solutions to address the data quality issues or challenges that health care practitioners are faced with during their daily jobs. Seven barriers were also identified as having an impact on the successful implementation of EMRs at health care institutions. These barriers, together with the data quality issues, influence the successful use of EMRs and should not be overlooked. From these barriers the study developed seven Critical Success Factors which can be used by the National Department of Health to improve the quality of EMRs. , Thesis (MCom) -- Faculty of Management and Commerce, 2019
- Full Text:
- Date Issued: 2019
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