Home is in the wandering
- Authors: Purdon, Bradley Michael
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435547 , vital:73167
- Description: Submitted in partial fulfilment of the requirements for the degree of Master of Arts in Creative Writing , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2024
- Full Text:
- Authors: Purdon, Bradley Michael
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435547 , vital:73167
- Description: Submitted in partial fulfilment of the requirements for the degree of Master of Arts in Creative Writing , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2024
- Full Text:
Fabrication of nanocatalysts as nanozymes-based biosensors for the detection of glucose and ascorbic acid
- Authors: Chavalala, Ridge Nhlamulo
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424501 , vital:72159
- Description: Embargoed. Expected release in 2025. , Thesis (MSc) -- Faculty of Science, Chemistry, 2023
- Full Text:
- Authors: Chavalala, Ridge Nhlamulo
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424501 , vital:72159
- Description: Embargoed. Expected release in 2025. , Thesis (MSc) -- Faculty of Science, Chemistry, 2023
- Full Text:
Quantifying the work demands of municipal waste workers to determine physical workload and perception
- Authors: Mamabolo, Lerato
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424267 , vital:72138
- Description: Objective: This study aimed to quantify physical and perceptual workloads through the nature of the work demands of municipal waste workers. Waste workers investigated were waste collectors, street sweepers [or public cleansers], drivers and mechanics. Methods: 154 City of Tshwane municipal waste workers volunteered to participate in the study. Observation-based tools were used to measure physical workload namely, the RAMP 1 and 2, REBA and RULA, and the hand-held dynamometer was used for pushing and pulling tasks. Subjective perceptual questionnaires were used to measure Perceptual load namely the NASA TLX and the QWIQ. Results: Differences in physical workload were evident between the occupation groups. Round bin collection was the waste collection type pertaining to the highest work-related risk of musculoskeletal development. Public cleansers and flatbed truck collectors' results displayed lower task-related risks associated in relation to the other occupations. Significant differences were found between the groups, particularly between waste collectors and drivers [had high workload outputs] in relation to public cleansers [low workload] for both perceptual workload tools. A significant difference was found in the mental and physical demands, as well as the organisational constraints and quantitative workloads. Mechanics showed no significant differences between groups but had high levels of frustration. Conclusion: A relationship between physical and perceived workload intensity is evident. development of musculoskeletal diseases and injury shall incur if measures are not reinstated. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2023
- Full Text:
- Authors: Mamabolo, Lerato
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424267 , vital:72138
- Description: Objective: This study aimed to quantify physical and perceptual workloads through the nature of the work demands of municipal waste workers. Waste workers investigated were waste collectors, street sweepers [or public cleansers], drivers and mechanics. Methods: 154 City of Tshwane municipal waste workers volunteered to participate in the study. Observation-based tools were used to measure physical workload namely, the RAMP 1 and 2, REBA and RULA, and the hand-held dynamometer was used for pushing and pulling tasks. Subjective perceptual questionnaires were used to measure Perceptual load namely the NASA TLX and the QWIQ. Results: Differences in physical workload were evident between the occupation groups. Round bin collection was the waste collection type pertaining to the highest work-related risk of musculoskeletal development. Public cleansers and flatbed truck collectors' results displayed lower task-related risks associated in relation to the other occupations. Significant differences were found between the groups, particularly between waste collectors and drivers [had high workload outputs] in relation to public cleansers [low workload] for both perceptual workload tools. A significant difference was found in the mental and physical demands, as well as the organisational constraints and quantitative workloads. Mechanics showed no significant differences between groups but had high levels of frustration. Conclusion: A relationship between physical and perceived workload intensity is evident. development of musculoskeletal diseases and injury shall incur if measures are not reinstated. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2023
- Full Text:
Baseline assessments for foundation phase reading in isiXhosa: a case study
- Authors: Nesi, Nolubabalo Amanda
- Date: 2022-10-14
- Subjects: Test of Early Reading Ability , Xhosa language , Reading (Elementary) Ability testing , Reading (Elementary) Social aspects South Africa Makhanda , Dynamic assessment (Education)
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/406320 , vital:70258
- Description: The main objective of this study was to investigate the use of baseline assessments for reading in isiXhosa in Foundation Phase. It focused on how teachers conduct baseline assessments, the assessment tools they use, and their responsiveness to baseline assessments data in terms of curriculum planning and practice, given the lack of explicit guidelines and tools specifically developed for African languages. The lack of clarity may lead teachers to assume that the same assessment approaches and practices apply across all official languages despite their linguistic and sociolinguistic differences. Vygotsky’s socio-cultural theory of learning and Biggs’ concept on constructive alignment are used as the theoretical and conceptual framework for this study. The premise for this study was that it would be problematic to apply similar assessment approaches and use the same tools for all official South African languages. The socio-cultural approach emphasises the importance of language learning and learning generally according to the context within which language occurs. The main research sites are two Quintile 1 schools in a township in Makhanda District. These schools have been receiving external intervention which includes a baseline assessment for isiXhosa reading from Non-Governmental Organisations (NGOs). This study sought to provide some insights on whether previous assessments have informed subsequent teaching practices. The findings of the study revealed that the most prominent tool for assessing reading in isiXhosa is Early Grade Reading Assessment (EGRA) and the tools used by external literacy interventions in the two schools were adapted versions of EGRA. Additionally, the subtasks in the baseline assessment tools used were aligned to the linguistic features of isiXhosa besides the nonsense word component. Furthermore, teachers were assessing and teaching reading at lower levels than expected in the grades 2 and 3 in this study. This study recommends that teachers respond to the baseline assessment data appropriately to develop reading competencies. In addition, there is need for an alignment between the linguistic features, the curriculum and strategies of teaching reading. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2022
- Full Text:
- Authors: Nesi, Nolubabalo Amanda
- Date: 2022-10-14
- Subjects: Test of Early Reading Ability , Xhosa language , Reading (Elementary) Ability testing , Reading (Elementary) Social aspects South Africa Makhanda , Dynamic assessment (Education)
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/406320 , vital:70258
- Description: The main objective of this study was to investigate the use of baseline assessments for reading in isiXhosa in Foundation Phase. It focused on how teachers conduct baseline assessments, the assessment tools they use, and their responsiveness to baseline assessments data in terms of curriculum planning and practice, given the lack of explicit guidelines and tools specifically developed for African languages. The lack of clarity may lead teachers to assume that the same assessment approaches and practices apply across all official languages despite their linguistic and sociolinguistic differences. Vygotsky’s socio-cultural theory of learning and Biggs’ concept on constructive alignment are used as the theoretical and conceptual framework for this study. The premise for this study was that it would be problematic to apply similar assessment approaches and use the same tools for all official South African languages. The socio-cultural approach emphasises the importance of language learning and learning generally according to the context within which language occurs. The main research sites are two Quintile 1 schools in a township in Makhanda District. These schools have been receiving external intervention which includes a baseline assessment for isiXhosa reading from Non-Governmental Organisations (NGOs). This study sought to provide some insights on whether previous assessments have informed subsequent teaching practices. The findings of the study revealed that the most prominent tool for assessing reading in isiXhosa is Early Grade Reading Assessment (EGRA) and the tools used by external literacy interventions in the two schools were adapted versions of EGRA. Additionally, the subtasks in the baseline assessment tools used were aligned to the linguistic features of isiXhosa besides the nonsense word component. Furthermore, teachers were assessing and teaching reading at lower levels than expected in the grades 2 and 3 in this study. This study recommends that teachers respond to the baseline assessment data appropriately to develop reading competencies. In addition, there is need for an alignment between the linguistic features, the curriculum and strategies of teaching reading. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2022
- Full Text:
Drifting towards death: a South African patient safety incident through an HFE Systems lens
- Authors: Agar, Sarah Leigh
- Date: 2022-10-14
- Subjects: Patients Safety measures , Medical errors Prevention , Human engineering , Medical care South Africa
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362716 , vital:65356
- Description: Patient Safety Incidents (PSI) are a frequent occurrence within the South African public healthcare system wherein a patient is unnecessarily maimed, harmed, killed, or put through significant trauma, emotional or physical. These incidents have a significant impact on the performance of the system and the well-being of individuals involved. Often PSI are the result of multiple system failings that provide the necessary preconditions for the PSI to occur. Thus, to provide appropriate patient safety recommendations to address and aid in the prevention of future PSI it is necessary to apply a systems approach to PSI analysis. A systems approach supports a ‘bigger picture’ view of an incident which includes looking beyond the immediate causes of a PSI and taking the different levels of the healthcare system into consideration during incident analysis. Human Factors and Ergonomics (HFE) is at its core a systems discipline and has been successfully applied to multiple fields including healthcare. HFE offers multiple incident analysis tools grounded in systems theory. The Life Esidimeni incident, a PSI that resulted in the death of 144 MHCU, is the biggest PSI in recent South African history and is therefore an important potential case study for the application of HFE systems tools within the South African healthcare context (an area that is lacking in existing literature). The objectives of this research were to (i) Systematically uncover the causal factors that led to the outcome of the of the Life Esidimeni incident; (ii) Identify critical faults, and gaps within the healthcare system that led to the Life Esidimeni PSI; and (iii) Provide proactive recommendations for future prevention of PSI. To fulfil these objectives a descriptive case study research method design was adopted using a qualitative systems-based tool, AcciMap. The application of AcciMap to Life Esidimeni enabled both the sharp end and blunt end causal factors that contributed to the outcome of the incident to be identified. Importantly this provided insight into the critical faults and gaps of the South African public healthcare system. The results of the AcciMap indicated that there were four main broad systemic faults in the system. These broad areas were categorized as key themes, which include: (i) competency, (ii) safeguards, (iii) time pressures, and (iv) vertical integration. From these key themes recommendations aimed at addressing the critical faults and gaps in the system and preventing future PSI were made. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2022
- Full Text:
- Authors: Agar, Sarah Leigh
- Date: 2022-10-14
- Subjects: Patients Safety measures , Medical errors Prevention , Human engineering , Medical care South Africa
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362716 , vital:65356
- Description: Patient Safety Incidents (PSI) are a frequent occurrence within the South African public healthcare system wherein a patient is unnecessarily maimed, harmed, killed, or put through significant trauma, emotional or physical. These incidents have a significant impact on the performance of the system and the well-being of individuals involved. Often PSI are the result of multiple system failings that provide the necessary preconditions for the PSI to occur. Thus, to provide appropriate patient safety recommendations to address and aid in the prevention of future PSI it is necessary to apply a systems approach to PSI analysis. A systems approach supports a ‘bigger picture’ view of an incident which includes looking beyond the immediate causes of a PSI and taking the different levels of the healthcare system into consideration during incident analysis. Human Factors and Ergonomics (HFE) is at its core a systems discipline and has been successfully applied to multiple fields including healthcare. HFE offers multiple incident analysis tools grounded in systems theory. The Life Esidimeni incident, a PSI that resulted in the death of 144 MHCU, is the biggest PSI in recent South African history and is therefore an important potential case study for the application of HFE systems tools within the South African healthcare context (an area that is lacking in existing literature). The objectives of this research were to (i) Systematically uncover the causal factors that led to the outcome of the of the Life Esidimeni incident; (ii) Identify critical faults, and gaps within the healthcare system that led to the Life Esidimeni PSI; and (iii) Provide proactive recommendations for future prevention of PSI. To fulfil these objectives a descriptive case study research method design was adopted using a qualitative systems-based tool, AcciMap. The application of AcciMap to Life Esidimeni enabled both the sharp end and blunt end causal factors that contributed to the outcome of the incident to be identified. Importantly this provided insight into the critical faults and gaps of the South African public healthcare system. The results of the AcciMap indicated that there were four main broad systemic faults in the system. These broad areas were categorized as key themes, which include: (i) competency, (ii) safeguards, (iii) time pressures, and (iv) vertical integration. From these key themes recommendations aimed at addressing the critical faults and gaps in the system and preventing future PSI were made. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2022
- Full Text:
Role of the Amathole Marine Protected Area in protecting vulnerable and threatened reef fish
- Phillips, Moraea Megan Taberer
- Authors: Phillips, Moraea Megan Taberer
- Date: 2021-10-29
- Subjects: Reef fishes South Africa Amathole District Municipality , Reef fishes Conservation South Africa Amathole District Municipality , Reef ecology South Africa Amathole District Municipality , Rare fishes South Africa Amathole District Municipality , Fish populations South Africa Amathole District Municipality Monitoring , Fish declines South Africa Amathole District Municipality , Underwater videography in wildlife monitoring South Africa Amathole District Municipality , Amathole Marine Protected Area , Petrus rupestris (Red Steenbras) , Polysteganus undulosus (Seventyfour seabream) , Chrysoblephus cristiceps (Daggerhead seabream)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192152 , vital:45200
- Description: The Amathole Marine Protected Area (MPA) was first established on a voluntary basis in the 1980’s by local ski-boat fishermen and comprised three small no-take areas near East London. In 2011, the areas received official recognition as the Amathole MPA and in 2019 an offshore extension was granted, increasing the area covered from 250 km2 to over 4000 km2. Though the inshore Amathole MPA has benefitted from decades longer of reprieve from fishing activity than the offshore MPA, it is limited in both its coastal and depth extent, placing in doubt its capacity to provide meaningful protection to several heavily threatened target species. Among the numerous endemic Sparids for which the Amathole region constitutes a vital portion of their distributional range are red steenbras (Petrus rupestris), seventyfour (Polysteganus undulosus), and dageraad (Chrysoblephus cristiceps). The stocks of all three species have collapsed and urgent intervention has been recommended to enable their recovery, including protection in strategically placed MPAs. The offshore Amathole MPA covers an extensive portion of prime habitat for these and other species, but no formal research has been carried out on fish assemblages in the region. As such, data to substantiate the benefits of the inshore Amathole MPA and to provide a baseline reference for the offshore MPA are lacking. This study made use of baited remote underwater stereo-video systems (stereo-BRUVS) to survey fish assemblages in the inshore Amathole MPA, adjacent inshore exploited areas, and exploited areas seaward of the inshore MPA. Sampling was carried out in 2015 and 2016, prior to the establishment of the offshore Amathole MPA. Fish assemblages from the inshore MPA were compared with those from adjacent exploited areas within the same depth range and from the offshore zone between 76 and 112 m depth. Within the inshore zone, biomass and abundance of target species were greater inside the MPA than in adjacent exploited areas, with vulnerable fisheries species showing the strongest response to protection. Offshore assemblages consisted of fewer species and fewer fish overall than those from the inshore zone but were dominated by larger individuals and those from higher trophic levels. The size of C. cristiceps was consistent across the sampled depth range but abundance of this species declined rapidly at depths of more than 60 m. Within the inshore zone, both abundance and size of C. cristiceps were greater in the MPA than in adjacent exploited areas. The size of P. rupestris was consistent across the sampled depth range and between protected and exploited areas, but this species was considerably more abundant offshore. Where P. rupestris was detected inshore it was almost exclusively in the MPA. No difference in the size or abundance of P. undulosus was found between the inshore MPA and inshore Abstract exploited areas, but both measures were found to increase with depth, with reproductive-sized adults restricted to the offshore zone. Despite its limited benefits for larger species, the inshore Amathole MPA is a critical refuge for smaller and more resident species like C. cristiceps, demonstrating the possibilities for conservation and management of endangered species in small MPAs. Baseline abundance and size measures for P. rupestris and P. undulosus recorded here indicate that the offshore extension of the Amathole MPA is ideally situated to protect spawner stock of these species and potentially contribute to the recovery of their stocks across a wider distributional range. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2021
- Full Text:
- Authors: Phillips, Moraea Megan Taberer
- Date: 2021-10-29
- Subjects: Reef fishes South Africa Amathole District Municipality , Reef fishes Conservation South Africa Amathole District Municipality , Reef ecology South Africa Amathole District Municipality , Rare fishes South Africa Amathole District Municipality , Fish populations South Africa Amathole District Municipality Monitoring , Fish declines South Africa Amathole District Municipality , Underwater videography in wildlife monitoring South Africa Amathole District Municipality , Amathole Marine Protected Area , Petrus rupestris (Red Steenbras) , Polysteganus undulosus (Seventyfour seabream) , Chrysoblephus cristiceps (Daggerhead seabream)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192152 , vital:45200
- Description: The Amathole Marine Protected Area (MPA) was first established on a voluntary basis in the 1980’s by local ski-boat fishermen and comprised three small no-take areas near East London. In 2011, the areas received official recognition as the Amathole MPA and in 2019 an offshore extension was granted, increasing the area covered from 250 km2 to over 4000 km2. Though the inshore Amathole MPA has benefitted from decades longer of reprieve from fishing activity than the offshore MPA, it is limited in both its coastal and depth extent, placing in doubt its capacity to provide meaningful protection to several heavily threatened target species. Among the numerous endemic Sparids for which the Amathole region constitutes a vital portion of their distributional range are red steenbras (Petrus rupestris), seventyfour (Polysteganus undulosus), and dageraad (Chrysoblephus cristiceps). The stocks of all three species have collapsed and urgent intervention has been recommended to enable their recovery, including protection in strategically placed MPAs. The offshore Amathole MPA covers an extensive portion of prime habitat for these and other species, but no formal research has been carried out on fish assemblages in the region. As such, data to substantiate the benefits of the inshore Amathole MPA and to provide a baseline reference for the offshore MPA are lacking. This study made use of baited remote underwater stereo-video systems (stereo-BRUVS) to survey fish assemblages in the inshore Amathole MPA, adjacent inshore exploited areas, and exploited areas seaward of the inshore MPA. Sampling was carried out in 2015 and 2016, prior to the establishment of the offshore Amathole MPA. Fish assemblages from the inshore MPA were compared with those from adjacent exploited areas within the same depth range and from the offshore zone between 76 and 112 m depth. Within the inshore zone, biomass and abundance of target species were greater inside the MPA than in adjacent exploited areas, with vulnerable fisheries species showing the strongest response to protection. Offshore assemblages consisted of fewer species and fewer fish overall than those from the inshore zone but were dominated by larger individuals and those from higher trophic levels. The size of C. cristiceps was consistent across the sampled depth range but abundance of this species declined rapidly at depths of more than 60 m. Within the inshore zone, both abundance and size of C. cristiceps were greater in the MPA than in adjacent exploited areas. The size of P. rupestris was consistent across the sampled depth range and between protected and exploited areas, but this species was considerably more abundant offshore. Where P. rupestris was detected inshore it was almost exclusively in the MPA. No difference in the size or abundance of P. undulosus was found between the inshore MPA and inshore Abstract exploited areas, but both measures were found to increase with depth, with reproductive-sized adults restricted to the offshore zone. Despite its limited benefits for larger species, the inshore Amathole MPA is a critical refuge for smaller and more resident species like C. cristiceps, demonstrating the possibilities for conservation and management of endangered species in small MPAs. Baseline abundance and size measures for P. rupestris and P. undulosus recorded here indicate that the offshore extension of the Amathole MPA is ideally situated to protect spawner stock of these species and potentially contribute to the recovery of their stocks across a wider distributional range. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2021
- Full Text:
Exploration of challenges in bringing traditional medicine into SA’s healthcare system, using medicinal plants for treatment of waterborne diarrhoeal diseases as a case study
- Authors: Keche, Priscilla
- Date: 2019
- Subjects: Traditional medicine -- South Africa , Waterborne infection -- South Africa , Diarrhea -- South Africa , Healers -- South Africa , Medical care -- Quality control , Medicinal plants -- South Africa , Diarrhea in children -- South Africa , World Health Organization
- Language: English
- Type: thesis , text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/118035 , vital:34588
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Biotechnology Innovation Centre (RUBIC), 2019.
- Full Text:
- Authors: Keche, Priscilla
- Date: 2019
- Subjects: Traditional medicine -- South Africa , Waterborne infection -- South Africa , Diarrhea -- South Africa , Healers -- South Africa , Medical care -- Quality control , Medicinal plants -- South Africa , Diarrhea in children -- South Africa , World Health Organization
- Language: English
- Type: thesis , text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/118035 , vital:34588
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Biotechnology Innovation Centre (RUBIC), 2019.
- Full Text:
The role of Stress Inducible Protein 1 (STI1) in the regulation of actin dynamics
- Authors: Beckley, Samantha Joy
- Date: 2015
- Subjects: Heat shock proteins , Molecular chaperones , Actin , Microfilament proteins , Cell migration , Adenosine triphosphatase , Metastasis
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/193941 , vital:45409
- Description: Stress-inducible protein 1 (STI1) otherwise known as Hop (Hsp70/Hsp90 organising protein) is a highly conserved abundant co-chaperone of the Hsp70 and Hsp90 chaperones. STI1 acts as an adapter protein, where it regulates the transfer of protein substrates from Hsp70 to Hsp90 during the assembly of a number of chaperone-client protein complexes. The role of STI1 associating independently with non-chaperone proteins has become increasingly prominent. Recent data from colocalisation and co-sedimentation analyses in our laboratory suggested a direct interaction between STI1 and the cytoskeletal protein, actin. However, there was a lack of information on the motifs which mediated this interaction, as well as the exact role of STI1 in the regulation of cytoskeletal dynamics. Two putative actin binding motifs, DAYKKK (within the TPR2A domain) and a polyproline region (after the DP1 domain), were identified in mammalian STI1. Our data from in vitro interaction studies including surface plasmon resonance and high speed co-sedimentation assays suggested that both TPR1 and TPR2AB were required for the STI1-actin interaction, and peptides corresponding to either the DAYKKK or the polyproline motif, alone or in combination, could not block the STI1-actin interaction. Full length mSTI1 was shown to have ATPase activity and when combined with actin an increase in ATPase activity was seen. Ex vivo studies using STI1 knockdown shRNA HEK293T cells and non-targeting control shRNA HEK293T cells showed a change of F-actin morphology as well as reduction in levels of actin-binding proteins profilin, cofilin and tubulin in the STI1 knockdown cells. These data extend our understanding of the role of STI1 in regulating actin dynamics and may have implications for cell migration. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2015
- Full Text:
- Authors: Beckley, Samantha Joy
- Date: 2015
- Subjects: Heat shock proteins , Molecular chaperones , Actin , Microfilament proteins , Cell migration , Adenosine triphosphatase , Metastasis
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/193941 , vital:45409
- Description: Stress-inducible protein 1 (STI1) otherwise known as Hop (Hsp70/Hsp90 organising protein) is a highly conserved abundant co-chaperone of the Hsp70 and Hsp90 chaperones. STI1 acts as an adapter protein, where it regulates the transfer of protein substrates from Hsp70 to Hsp90 during the assembly of a number of chaperone-client protein complexes. The role of STI1 associating independently with non-chaperone proteins has become increasingly prominent. Recent data from colocalisation and co-sedimentation analyses in our laboratory suggested a direct interaction between STI1 and the cytoskeletal protein, actin. However, there was a lack of information on the motifs which mediated this interaction, as well as the exact role of STI1 in the regulation of cytoskeletal dynamics. Two putative actin binding motifs, DAYKKK (within the TPR2A domain) and a polyproline region (after the DP1 domain), were identified in mammalian STI1. Our data from in vitro interaction studies including surface plasmon resonance and high speed co-sedimentation assays suggested that both TPR1 and TPR2AB were required for the STI1-actin interaction, and peptides corresponding to either the DAYKKK or the polyproline motif, alone or in combination, could not block the STI1-actin interaction. Full length mSTI1 was shown to have ATPase activity and when combined with actin an increase in ATPase activity was seen. Ex vivo studies using STI1 knockdown shRNA HEK293T cells and non-targeting control shRNA HEK293T cells showed a change of F-actin morphology as well as reduction in levels of actin-binding proteins profilin, cofilin and tubulin in the STI1 knockdown cells. These data extend our understanding of the role of STI1 in regulating actin dynamics and may have implications for cell migration. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2015
- Full Text:
- «
- ‹
- 1
- ›
- »