An in-silico study of the type II NADH: Quinone Oxidoreductase (ndh2). A new anti-malaria drug target
- Authors: Baye, Bertha Cinthia
- Date: 2022-10-14
- Subjects: Malaria , Plasmodium , Molecular dynamics , Computer simulation , Quinone , Antimalarials , Molecules Models , Docking , Drugs Computer-aided design
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/365633 , vital:65767 , DOI https://doi.org/10.21504/10962/365633
- Description: Malaria is caused by Plasmodium parasites, spread to people through the bites of infected female Anopheles mosquitoes. This study focuses on all 5 (Plasmodium falciparum, Plasmodium knowlesi, Plasmodium malariae, Plasmodium ovale and Plasmodium vivax) parasites that cause malaria in humans. Africa is a developing continent, and it is the most affected with an estimation of 90% of more than 400 000 malaria-related deaths reported by the World Health Organization (WHO) report in 2020, in which 61% of that number are children under the ages of five. Malaria resistance was initially observed in early 1986 and with the progression of time anti-malarial drug resistance has only increased. As a result, there is a need to study the malarial proteins mechanism of action and identify alternative treatment strategies for this disease. Type II NADH: quinone oxidoreductase (NDH2) is a monotopic protein that catalyses the electron transfer from NADH to quinone via FAD without a proton-pumping activity, and functions as an initial enzyme, either in addition to or as an alternative to proton-pumping NADH dehydrogenase (complex I) in the respiratory chain of bacteria, archaea, and fungal and plant mitochondrial. The structures for the Plasmodium knowlesi, Plasmodium malariae, Plasmodium ovale and Plasmodium vivax were modelled from the crystal structure of Plasmodium falciparum (5JWA). Compounds from the South African natural compounds database (SANCDB) were docked against both the NDH2 crystal structure and modelled structures. By performing in silico screening the study aimed to find potential compounds that might interrupt the electron transfer to quinone therefore disturbing the enzyme‟s function and thereby possibly eliminating the plasmodium parasite. CHARMM-GUI was used to create the membrane (since this work is with membrane-bound proteins) and to orient the protein on the membrane using OPM server guidelines, the interface produced GROMACS topology files that were used in molecular dynamics simulations. Molecular dynamics simulations were performed in the Centre for high performance computing (CHPC) cluster under the CHEM0802 project and the trajectories produced were further analysed. In this work not only were hit compounds from SANCDB identified, but also differences in behaviour across species and in the presence or absence of the membrane were described. This highlights the need to include the correct protein environment when studying these systems. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2022
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A sociological study of menstrual hygiene management in schools in the Makana District, Eastern Cape, South Africa
- Authors: Bloem, Sharon Gretchen
- Date: 2021-10-29
- Subjects: Schoolgirls Health and hygiene South Africa Makhanda , Menstruation Social aspects South Africa Makhanda , Health behavior in adolescence South Africa Makhanda , Teenage girls Health and hygiene South Africa Makhanda , Teenage girls Physiology South Africa Makhanda , Life skills South Africa Makhanda
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/189974 , vital:44952
- Description: Appropriate menstrual hygiene management is affected by taboos and secrecy surrounding menstruation. Inadequate information and knowledge about menstruation and lack of resources for managing menstruation hygienically have impacted adolescent girls on a psychosocial and physical level. Consequently, the dignity of schoolgirls during the onset of menstruation through puberty and adolescent phases is jeopardised. It is therefore imperative to understand the normal physiological changes that school-going children from Grades 6 to 12 undergo and acknowledge how these changes affect their education within the school environment. In the Makana district, Eastern Cape, South Africa, the topic of menstrual hygiene management is under-researched at schools and, therefore, this research explores school educators’ perceptions of menstrual hygiene management at primary, secondary and private schools in this area. Schools have a responsibility to create a safe environment for learners’ education, which includes the topic of menstruation without discriminating against or labelling menstruating girls. This research found that the topic of menstruation is only discussed in Life Science and Life Orientation subjects and only covers the physiological changes of the body. These subjects, however, exclude the topic of menstrual hygiene management. This study found that several schools in the Makana district are not adequately equipped to provide for the needs of menstruating girls and therefore educators need to be prepared to deal with menstruation in the school context. The educators are also required to go for regular training or workshops to equip them to manage menstruation in a school environment. The infrastructure of a school includes water and sanitation which are components of menstrual hygiene management. The infrastructure at the participating public schools was inadequate and therefore impacted on the menstrual hygiene management for the girls. The unavailability of water, the non-existence of a sick room and lack of a waste disposal system at some public schools presented further challenges for menstrual hygiene management. A qualitative research method was applied in this study and a symbolic interactionism approach was used. , Thesis (MA) -- Faculty of Humanities, Sociology, 2021
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An analysis of the medicines regulatory environment in sub-Saharan Africa and the African Medicines Regulatory Harmonization Initiative
- Authors: Feka, Cedric Nyahchong
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192408 , vital:45223
- Description: Thesis (MSc (Pharm)) -- Faculty of Pharmacy, Pharmacy, 2021
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The digital disruption of journalistic identity at the South African Broadcasting Corporation (SABC)
- Authors: Oosthuizen, Nina-Celeste
- Date: 2021-10-29
- Subjects: South African Broadcasting Corporation , Journalism Technological innovations South Africa , Digital media South Africa , Online journalism South Africa , Identity (Psychology) in mass media , Public broadcasting Political aspects South Africa , Journalistic ethics , Mass media Employees , Mass media Objectivity South Africa , Journalism Social aspects South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192303 , vital:45214
- Description: This research investigates changes in journalistic identity with the introduction of online journalism practices in the SABC newsrooms. The study is a qualitative analysis of in-depth interviews with 6 SABC journalists. It focuses on SABC journalists who embrace a professional identity. Participants were selected from all three of the SABC newsrooms: Television, Radio and Digital News. The research reveals that SABC News journalists are - due to digital production workflows - increasingly pressured to work on their own in the field, with additional responsibilities and fewer resources, while taking on editorial duties and managing corporate and personal social media accounts. As the roles of SABC journalists become digitally disrupted and blur with those of technicians, editors and marketers, I ask how this might in turn disrupt journalistic identity. The interviews reveal how these SABC journalists have always understood their identities and values in opposition to those of corporate SABC leadership. While they, the journalists, sometimes allowed editors to change their stories, this was not done without resistance; it was a strategic compromise, since they understood the greater balance of their work to serve the public. However, this notion of being separate from the corporate identity has been disrupted through digital and social media, as it conflates their identity with the SABC brand. Journalists experience this acutely through ‘trolling’. Yet, conversely, some are also able to retain a sense of an independent professional identity through a direct relationship with the public on social media. Another key finding was that digital media disrupts the centrality of primary journalistic research or ‘legwork’, and instead, journalists increasingly spend time on the selection and repackaging of user-generated content. As some journalists are allocated more deskwork they experience a loss of status among their colleagues. The diminished role of journalists’ primary research, or eye-witness testimony, has created tensions in journalistic identity and what it means to be a ‘real’ journalist. Despite their concerns for the danger of reporting in the field in South Africa, SABC journalists considered such verification work crucial to their identity and what it means to be a journalist. , Thesis (MA) -- Faculty of Humanities, Journalism and Media Studies, 2021
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