List of Titles

Selective and sensitive electrochemical detection of the Human Epidermal Growth Receptor 2 breast cancer biomarker, using Co (II) phthalocyanine-nanoparticle based platforms

- Centane, Sixolile Sibongiseni

Authors: Centane, Sixolile Sibongiseni
Date: 2024-10-11
Subjects: Electrochemical sensors , HER-2 protein , Breast Cancer , Biochemical markers , Phthalocyanines , Nanoparticles
Language: English
Type: Academic theses , Doctoral theses , text
Identifier: http://hdl.handle.net/10962/466569 , vital:76753 , DOI https://doi.org/10.21504/10962/466570
Description: Breast cancer is the world’s leading cause of cancer related deaths in women worldwide. The main reason lies in its late detection, mostly in the metastatic stage resulting in poor after-therapy prognosis, despite advances in methods of diagnosis and therapy. The reason for late-stage detection, is because breast cancer like any other cancers is asymptomatic in its early stages. Significant and characterizable features present in the later stages. Furthermore, conventional methods for breast cancer detection are more useful in the identification of the phenotypic features of cancer cells that arise at a later stage of the disease. Another issue with conventional methods where cancer diagnosis is concerned is that they tend to be specialist-dependent, time consuming and costly. Thus, easy, fast and inexpensive detection methods need to be developed urgently. Biomarker-based cancer diagnosis has emerged as one of the most promising strategies for early diagnosis, monitoring disease progression, and subsequent cancer treatment. This thesis focuses on the design and development of novel electrochemical biosensor platforms towards the low cost, efficient, sensitive and simple detection of early-stage breast cancer biomarker, human epidermal growth factor 2 (HER2). The electrochemical method is preferred because of its moderate cost, rapid response, ease of operation, readily quantifiable signal as well as high sensitivity and selectivity with lower detection limits. This thesis reports on two strategies towards signal amplification and sensitive detection of HER2, namely signal based amplification and target-based amplification. The former focuses on electrode or transducer modification techniques for improved signal to noise ratio. In which case; novel nanocomposites of phthalocyanines, graphene quantum dots, gold nanoparticles and cerium oxide nanoparticles are used for electrode modification for signal amplification and biorecognition element immobilization. The biorecognition elements of choice, are an aptamer and antibody known to be specific to the HER2 antigen for an enhanced sensor sensitivity and specificity. The second strategy focuses on increasing the number of detectable targets on the electrode surface towards enhanced sensitivity, precision and sensor accuracy. In which case; the performance of the aptamer and the antibody as recognition elements was explored. Furthermore, the effect of arrangement of these recognition elements on the electrode surface is investigated and reported upon. The strategies covered in this thesis are expected to result in novel biosensor platforms that can detect the HER2 biomarker with high precision, reproducibility, sensitivity and stability; towards low cost and effective early-stage breast cancer diagnostic tools. , Thesis (PhD) -- Faculty of Science, Chemistry, 2024
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Date Issued: 2024-10-11

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The perception of school management teams and Teachers about their role to implement the national school nutrition programme in the Sisonke District in KwaZulu-Natal

- Zulu, Xolani Wycliff

Authors: Zulu, Xolani Wycliff
Date: 2022-04
Subjects: School management teams , nutrition -- Study and teaching -- KwaZulu-Natal
Language: English
Type: Doctoral's theses , Thesis
Identifier: http://hdl.handle.net/10948/56102 , vital:55420
Description: This study focused on the perception of School Management Teams and Teachers about their role to implement the national school nutrition programme in the Sisonke District in KwaZulu-Natal (South Africa). The national school nutrition programme like any other projects in the country, faces some changes in the District which seem to interfere with the role of stakeholders in implementing the programme. This study explored the following research question with the set of secondary research questions: What are the perceptions of School Management Teams and Teachers in the Sisonke District of KwaZulu-Natal about their role in National School Nutrition Programme? • What perceptions do teachers and school managers have on the implementation of National School Nutrition Programme? • What challenges do Teachers and School Managers have to enhance the objectives of the National School Nutrition Programme? Both Teachers and School Managers were conveniently and purposively selected. For the generation of data, semi-structured interviews were used which were tape-recorded which were followed by transcription of data with interim analysis. Data was also generated through the use document analysis. Thematic analysis was used to analyse the data, which was theoretically framed by Maslow’s theory of motivation, Hertzberg two-factor motivation theory, Lawler and Porter’s Expectancy theory of motivation and the Situational theory of Hersey and Blanchard through which, I lens the study and managed to explain the findings. The study findings revealed that most Teachers and School Managers perceived national school nutrition programme as an important programme in the context of Umzimkhulu as it isa deep rural area. Despite the positive perceptions of Teachers and School Managers about their role, the study findings further revealed that they are facing some challenges in the implementation of the programme. The findings have several implications for poverty alleviation in the Sisonke District, job creation and improved learner’s attendance, improved health conditions of learners. They also highlighted the weaknesses of the DBE official’s nutrition sub-directorate about role players of the programme. Both stakeholders in fulfilling their role, should take into consideration the constitutional rights of learners as some of them are in Maslow’s hierarchy of needs. , Thesis (PhD) -- Faculty of Education, 2022
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Date Issued: 2022-04

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Managing the corporate reputation of a transforming organisation: a study of multimedia University of Kenya

- Mtange, Margaret Mulekani

Authors: Mtange, Margaret Mulekani
Date: 2020-04
Subjects: Port Elizabeth (South Africa) , Eastern Cape (South Africa) , South Africa
Language: English
Type: Doctoral theses , text
Identifier: http://hdl.handle.net/10948/55042 , vital:48830
Description: Universities, like other organisations, are challenged to manage corporate identity and reputation to maintain a competitive edge. Few studies in Africa focus on the impact of internal communication on corporate identity and reputation management during organisational transformation. Thus, this study interrogated how a transforming and complex university in Kenya managed the corporate identity, image and reputation for competitive advantage. The purpose of the study was to establish how Multimedia University of Kenya (MMU) management communicated with its employees to nurture the corporate identity, image and reputation during a ten-year period of transformation. The study draws insights from in-depth interviews with 23 university management members, 178 self-administered questionnaires through random stratification of MMU employees, and content review of two MMU strategic plans ranging from 2011 to 2021. The findings suggest that MMU management used formal and interactive channels to create awareness of organisational transformation. These channels included face-to-face engagements, formal meetings and mediated communication through telephone conversations and intranet that facilitated record keeping. The management engaged in top-down and bottom-up communication to build employee trust, while employees expressed reservations of bottom-up information accuracy, suggesting information exclusion. On the contrary, the employees preferred digital and interpersonal channels for internal communication, which included telephone conversations, social media, personal visits by MMU management, and university events. Publicity activities were to disseminate information to prospective students and sponsors through selected private television, vernacular radio, and MMU radio stations, traditional print media, and the MMU website to communicate the MMU brand and image. Employees acknowledged that MMU brands itself as a leader in telecommunication, technology, mechanical, manufacturing, and media training through state-of-the-art equipment, robust faculty, and problem-solving ventures through research. The current study proposes stronger employee involvement, executive packaging and positioning, coaching successful faculty members as brand ambassadors, retraining employees, and employee participation in policy formulation to enhance new culture. In addition, these opositions will enhance the corporate identity, brand, as well as university performance, product quality, policy and processes to enrich the corporate reputation during transformation. The findings challenge university management to actively engage the corporate communication function, emphasise employee participation, and focus on the continual improvement of corporate identity and reputation management practices for competitive advantage. The current study proposes a participative, multi-layered and multi-dimensional communication model for efficient and excellent internal communication. The current study recommends that corporate communication practitioner be strategically positioned to coordinate employee engagement, utilise appropriate communication channels and counsel university management on corporate identity, image and reputation management during organisational transformation. , Thesis (PhD) -- Faculty of Humanities, School of Language, Media and Communication, 2020
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Date Issued: 2020-04

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