An unsung dialogue: music, society and the history of The Flames
- Authors: Park, Duncan Keith
- Date: 2020
- Subjects: The Flames (Musical group) , Fataar, Steve , Durban (South Africa) -- History , Durban (South Africa) -- Race relations , Musical groups -- South Africa , Music -- Social aspects -- South Africa , Music -- Social aspects -- South Africa -- Durban , South Africa -- Politics and government -- 1961-1978
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/169632 , vital:41780
- Description: This research aims to examine the relationship between music and social conditions within the context of apartheid . The focus area is the city of Durban, specifically 1960-1970. A case study of the multi-racial Durban-based band, The Flames 1963–1972) will be used to rigorously assess the extent to which social conditions and music can affect each other in both directions. This will also be the first coherent and historical narrative of the band, The Flames. As such, the thesis aims to make an original contribution to this field of music history, while it will provide the very first academic discussion of The Flames. South Africa during apartheid, with its racialised legislation provides an ideal context to scrutinize the effects (both potential and real) of music on social conditions. The Flames provide a particularly relevant case point; being a collective of “mixed-race” individuals classified as Coloured performing for a multi-racial fan- base. This means that at their concerts, audience members would have frequently been from a variety of different racial backgrounds. This would have been in direct contravention to the National Party‟s (NP) Separate Amenities Act as well as the policy of “separate development”, the cornerstone of apartheid. The thesis will firstly contextualise the research within the broader, global historical context, as well as in the existing theoretical literature which focuses on the relationship between music, politics and society. Firstly, this will entail a brief historical account of popular music around the world during the twentieth century. Through this we will observe both the ways in which the development and popularisation of various forms of popular music came to be shaped in a particular way, as well as the ways in which the public received this music. This will include an examination of positive responses to certain kinds of popular music which resonated and became popular with various sectors of society. Conversely, it will also closely examine the backlashes against these musical forms, and attempt to identify why certain sectors of the public were deeply opposed to particular kinds of popular music which may have been perceived to embody certain values and meanings. This will be conducted by studying various forms of popular music from the turn of the twentieth century into the early 1970s with a cross-cultural, global perspective, examining particular historical instances and existing theories relating to these instances. Chapter one will then shift from a global perspective and will situate the research within the South African musical context specifically. This will include an account of the development of South African popular music through the twentieth century in relation to the country's politics. Through examining this relationship, the dialogue between historical case studies and theoretical literature will continue, in which existing theories relating to the relationship between music, politics and society will be discussed. This theoretical literature will be made use of in the final section of the paper in order to make sense of The Flames and their role in South African history. The second section of this thesis will focus on the socio-historical context of the city of Durban under apartheid. The author will contextualise the research through a narrative historical retelling of Durban‟s social history, focusing on both political and social public mobilisation and the role of cultural spaces and practices within these his torical moments and their relevant structures. Attention will be paid to the development of segregation and apartheid within Durban specifically, while racial relations will also be fo cused upon. This will provide the reader with the necessary background required in order to meaningfully understand how the band The Flames came into existence, became popular, and whether or not they had any meaningful effect on their historical context. The section on Durban's history will be followed by a brief account of Durban's Coloured community's history, as well as a discussion around Coloured identity more broadly within the South African context. This will be vital to the research due to the fact that the musicians of The Flames were classified as Coloured under apartheid, and such an historical background will be critical to understanding the social, political and economic context of the band. Finally, the thesis will end with the written history of The Flame . This final section will provide an account of The Flames' history in which the band will be made sense of within the context of Durban during apartheid, as well as within the context of South African music, and the globalised context of popular music. By situating the band within these three contexts, we can truly assess both how they were shaped by their contexts, and whether they had any effect on these contexts of which they were a part. In this section those existing theories around the relationship between music, politics and society will be discussed and assessed in order to determine how useful a framework they may be for understanding popular music in certain historical contexts. In this way, the research aims to make a meaningful contribution to the study of music and its socio-political role throughout history, as well as to the broader understanding of Durban's history. Specifically, the role of music in Durban's history is being examined, and the researcher hopes that this work can begin to open up new discussions around the importance of studying music in Durban, and South Africa's history. Additionally, this thesis will open up a new area of research into a band which, until this point, has not been engaged with in the field of music history whatsoever. .
- Full Text:
- Date Issued: 2020
- Authors: Park, Duncan Keith
- Date: 2020
- Subjects: The Flames (Musical group) , Fataar, Steve , Durban (South Africa) -- History , Durban (South Africa) -- Race relations , Musical groups -- South Africa , Music -- Social aspects -- South Africa , Music -- Social aspects -- South Africa -- Durban , South Africa -- Politics and government -- 1961-1978
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/169632 , vital:41780
- Description: This research aims to examine the relationship between music and social conditions within the context of apartheid . The focus area is the city of Durban, specifically 1960-1970. A case study of the multi-racial Durban-based band, The Flames 1963–1972) will be used to rigorously assess the extent to which social conditions and music can affect each other in both directions. This will also be the first coherent and historical narrative of the band, The Flames. As such, the thesis aims to make an original contribution to this field of music history, while it will provide the very first academic discussion of The Flames. South Africa during apartheid, with its racialised legislation provides an ideal context to scrutinize the effects (both potential and real) of music on social conditions. The Flames provide a particularly relevant case point; being a collective of “mixed-race” individuals classified as Coloured performing for a multi-racial fan- base. This means that at their concerts, audience members would have frequently been from a variety of different racial backgrounds. This would have been in direct contravention to the National Party‟s (NP) Separate Amenities Act as well as the policy of “separate development”, the cornerstone of apartheid. The thesis will firstly contextualise the research within the broader, global historical context, as well as in the existing theoretical literature which focuses on the relationship between music, politics and society. Firstly, this will entail a brief historical account of popular music around the world during the twentieth century. Through this we will observe both the ways in which the development and popularisation of various forms of popular music came to be shaped in a particular way, as well as the ways in which the public received this music. This will include an examination of positive responses to certain kinds of popular music which resonated and became popular with various sectors of society. Conversely, it will also closely examine the backlashes against these musical forms, and attempt to identify why certain sectors of the public were deeply opposed to particular kinds of popular music which may have been perceived to embody certain values and meanings. This will be conducted by studying various forms of popular music from the turn of the twentieth century into the early 1970s with a cross-cultural, global perspective, examining particular historical instances and existing theories relating to these instances. Chapter one will then shift from a global perspective and will situate the research within the South African musical context specifically. This will include an account of the development of South African popular music through the twentieth century in relation to the country's politics. Through examining this relationship, the dialogue between historical case studies and theoretical literature will continue, in which existing theories relating to the relationship between music, politics and society will be discussed. This theoretical literature will be made use of in the final section of the paper in order to make sense of The Flames and their role in South African history. The second section of this thesis will focus on the socio-historical context of the city of Durban under apartheid. The author will contextualise the research through a narrative historical retelling of Durban‟s social history, focusing on both political and social public mobilisation and the role of cultural spaces and practices within these his torical moments and their relevant structures. Attention will be paid to the development of segregation and apartheid within Durban specifically, while racial relations will also be fo cused upon. This will provide the reader with the necessary background required in order to meaningfully understand how the band The Flames came into existence, became popular, and whether or not they had any meaningful effect on their historical context. The section on Durban's history will be followed by a brief account of Durban's Coloured community's history, as well as a discussion around Coloured identity more broadly within the South African context. This will be vital to the research due to the fact that the musicians of The Flames were classified as Coloured under apartheid, and such an historical background will be critical to understanding the social, political and economic context of the band. Finally, the thesis will end with the written history of The Flame . This final section will provide an account of The Flames' history in which the band will be made sense of within the context of Durban during apartheid, as well as within the context of South African music, and the globalised context of popular music. By situating the band within these three contexts, we can truly assess both how they were shaped by their contexts, and whether they had any effect on these contexts of which they were a part. In this section those existing theories around the relationship between music, politics and society will be discussed and assessed in order to determine how useful a framework they may be for understanding popular music in certain historical contexts. In this way, the research aims to make a meaningful contribution to the study of music and its socio-political role throughout history, as well as to the broader understanding of Durban's history. Specifically, the role of music in Durban's history is being examined, and the researcher hopes that this work can begin to open up new discussions around the importance of studying music in Durban, and South Africa's history. Additionally, this thesis will open up a new area of research into a band which, until this point, has not been engaged with in the field of music history whatsoever. .
- Full Text:
- Date Issued: 2020
Shoutfighting and other fiction
- Authors: Rasmenike, Nonqubela Evelyn
- Date: 2020
- Subjects: South African fiction (English) -- 21st century , Xhosa fiction -- 21st century
- Language: English , Xhosa
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/144500 , vital:38351
- Description: This document consists of three parts: Part A: English Half Thesis (Creative Work) ; Part B: IsiXhosa Half Thesis (Creative Work) ; Part C: Portfolio.
- Full Text:
- Date Issued: 2020
- Authors: Rasmenike, Nonqubela Evelyn
- Date: 2020
- Subjects: South African fiction (English) -- 21st century , Xhosa fiction -- 21st century
- Language: English , Xhosa
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/144500 , vital:38351
- Description: This document consists of three parts: Part A: English Half Thesis (Creative Work) ; Part B: IsiXhosa Half Thesis (Creative Work) ; Part C: Portfolio.
- Full Text:
- Date Issued: 2020
Synthesis, photophysicochemical properties and photodynamic therapy activities of indium and zinc phthalocyanines when incorporated into Pluronic polymer micelles
- Authors: Motloung, Banele Mike
- Date: 2020
- Subjects: Indium , Zinc , Phthalocyanines , Polymers , Photochemotherapy , Micelles
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/167529 , vital:41489
- Description: This thesis reports on the syntheses, photophysicochemical properties and photodynamic therapy activities of symmetrical metallophthalocyanines (MPcs) when alone or when incorporated into Pluronic polymer micelles. The Pcs contain either zinc or indium as central metals and have phenyldiazenylphenoxy, pyridine-2-yloxy and benzo[d]thiazol-2-ylthio as ring substituents. Spectroscopic and microscopic techniques were used to confirm the formation MPcs with micelles. The photophysics and photochemistry of the Pcs were assessed when alone and with micelles. All the studied Pcs showed good photophysicochemical behavior with relatively high triplet and singlet oxygen quantum yields corresponding to their low fluorescence quantum yields. The Pcs with indium in their central cavity exhibited higher triplet and singlet oxygen quantum yields in comparison to their zinc counterparts due to the heavy atom effect obtained from the former. The in vitro dark cytotoxicity and photodynamic therapy of the Pc complexes and conjugates against MCF7 cells was tested. All studied Pc complexes alone and with micelles showed minimum dark toxicity making them applicable for PDT. All complexes displayed good phototoxicity < 50% cell viability (except for complex 2 > 50% cell viability) at concentrations ≤100 μg/mL, however the conjugates showed < 45% cell viability at concentrations ≤ 100 μg/mL, probably due to the small micellar size and EPR effect. The findings from this work show the importance of incorporating photosensitizers such as phthalocyanines into Pluronic polymers micelles and making them water soluble and ultimately improving their photodynamic effect.
- Full Text:
- Date Issued: 2020
- Authors: Motloung, Banele Mike
- Date: 2020
- Subjects: Indium , Zinc , Phthalocyanines , Polymers , Photochemotherapy , Micelles
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/167529 , vital:41489
- Description: This thesis reports on the syntheses, photophysicochemical properties and photodynamic therapy activities of symmetrical metallophthalocyanines (MPcs) when alone or when incorporated into Pluronic polymer micelles. The Pcs contain either zinc or indium as central metals and have phenyldiazenylphenoxy, pyridine-2-yloxy and benzo[d]thiazol-2-ylthio as ring substituents. Spectroscopic and microscopic techniques were used to confirm the formation MPcs with micelles. The photophysics and photochemistry of the Pcs were assessed when alone and with micelles. All the studied Pcs showed good photophysicochemical behavior with relatively high triplet and singlet oxygen quantum yields corresponding to their low fluorescence quantum yields. The Pcs with indium in their central cavity exhibited higher triplet and singlet oxygen quantum yields in comparison to their zinc counterparts due to the heavy atom effect obtained from the former. The in vitro dark cytotoxicity and photodynamic therapy of the Pc complexes and conjugates against MCF7 cells was tested. All studied Pc complexes alone and with micelles showed minimum dark toxicity making them applicable for PDT. All complexes displayed good phototoxicity < 50% cell viability (except for complex 2 > 50% cell viability) at concentrations ≤100 μg/mL, however the conjugates showed < 45% cell viability at concentrations ≤ 100 μg/mL, probably due to the small micellar size and EPR effect. The findings from this work show the importance of incorporating photosensitizers such as phthalocyanines into Pluronic polymers micelles and making them water soluble and ultimately improving their photodynamic effect.
- Full Text:
- Date Issued: 2020
‘Regulated Flexibility’ and labour market regulation: a case Study of Twizza Soft Drinks in the Eastern Cape, South Africa
- Authors: Flatau, Scott
- Date: 2020
- Subjects: Labor market -- South Africa , Labor laws and legislation -- South Africa , Industrial relations -- South Africa -- Case studies
- Language: English
- Type: text , Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/141288 , vital:37959
- Description: Following the negotiated settlement, which led to the ANC assuming power in South Africa in1994, debates concerning the nature of the South African labour market ensued between policy makers and economists alike. Central to understanding the South African labour market was the policy objective of regulated flexibility that has guided the formation of labour legislation in the post-1994 period, including the Labour Relations Act of 1995, the Basic Conditions of Employment Act of 1997, the Employment Equity Act of 1998 and the Skills Development Act of 1998. Regulated flexibility attempts to accommodate the interests of the employer for flexibility and the interests of the employee in regulation or security. These four Acts and the relevant provisions contained within them are the central focus of this research paper, in particular how they affect the case study firm Twizza Soft Drinks. An interpretivist approach was utilised as the preferred research methodology with in-depth, semi-structured interviews being the primary source of data collection. This research paper attempts to situate more clearly the impact of South Africa’s macro-economic policies since 1994 on labour market policy and undertakes an exploration of internal dynamics of firms in response to exogenous factors, such as government regulation. The key finding suggest that some Acts (BCEA, LRA) do not impose a significant burden on the firm and some provisions can lead to beneficial outcomes such as business modernisation and the adoption of formal Human Resource Practices. Conversely, some provisions contained in the EEA increase the administrative burden and therefore increase the indirect cost on the firm.
- Full Text:
- Date Issued: 2020
- Authors: Flatau, Scott
- Date: 2020
- Subjects: Labor market -- South Africa , Labor laws and legislation -- South Africa , Industrial relations -- South Africa -- Case studies
- Language: English
- Type: text , Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/141288 , vital:37959
- Description: Following the negotiated settlement, which led to the ANC assuming power in South Africa in1994, debates concerning the nature of the South African labour market ensued between policy makers and economists alike. Central to understanding the South African labour market was the policy objective of regulated flexibility that has guided the formation of labour legislation in the post-1994 period, including the Labour Relations Act of 1995, the Basic Conditions of Employment Act of 1997, the Employment Equity Act of 1998 and the Skills Development Act of 1998. Regulated flexibility attempts to accommodate the interests of the employer for flexibility and the interests of the employee in regulation or security. These four Acts and the relevant provisions contained within them are the central focus of this research paper, in particular how they affect the case study firm Twizza Soft Drinks. An interpretivist approach was utilised as the preferred research methodology with in-depth, semi-structured interviews being the primary source of data collection. This research paper attempts to situate more clearly the impact of South Africa’s macro-economic policies since 1994 on labour market policy and undertakes an exploration of internal dynamics of firms in response to exogenous factors, such as government regulation. The key finding suggest that some Acts (BCEA, LRA) do not impose a significant burden on the firm and some provisions can lead to beneficial outcomes such as business modernisation and the adoption of formal Human Resource Practices. Conversely, some provisions contained in the EEA increase the administrative burden and therefore increase the indirect cost on the firm.
- Full Text:
- Date Issued: 2020
Demographic change in the Upper Tsitsa Catchment: the integration of census and land cover data for 2001 and 2011
- Authors: Hodgson, Danuta Lorina
- Date: 2018
- Subjects: Land use -- South Africa -- Eastern Cape , Watersheds -- South Africa -- Eastern Cape , Thematic maps -- South Africa -- Eastern Cape , Population density -- South Africa -- Eastern Cape , Mzimvubu River Watershed (South Africa) -- Census , Upper Tsitsa Catchment (South Africa)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/57296 , vital:26870
- Description: The purpose of this research was to determine if the integration of census and land cover data could provide evidence of spatial patterns and temporal change for the Upper Tsitsa Catchment. This thesis contributed to academic literature with regards to dasymetric mapping and provided a database for the Ntabelanga and Laleni Ecological Infrastructure Project. The study took place in the Upper Tsitsa Catchment which is located in the north-eastern region of the Eastern Cape and falls within the uMzimvubu Catchment. South Africa National Population Censuses for 2001 and 2011 and the National Land Cover data sets for 2000 and 2013/14 were used to create dasymetric maps depicting demographic changes over time for the catchment area. Spatial statistics were performed on the dasymetric and choropleth map to determine the accuracy of the data that was created. From the results, it was found that although the statistics were skewed, the method was more accurate in displaying the population densities, which was noted during the sampling process of the spatial analysis. It was found that there had been a decrease in the population density within the catchment. This affected the density of several other variables such as population race group, language and employment status statistics as decreases in these values could be due to individuals migrating out of the catchment as well as socio-economic upliftment, such as having better access to services. The use of dasymetric mapping allowed an accurate representation of the population density from the census data to be created. The results of the dasymetric mapping were more accurate as they depicted where the population within the enumeration areas were located, and recognised that some areas were populated while some areas were not. To conclude, it was found that using dasymetric mapping provided reliable and useful data about population density and enables comparison over time.
- Full Text:
- Date Issued: 2018
- Authors: Hodgson, Danuta Lorina
- Date: 2018
- Subjects: Land use -- South Africa -- Eastern Cape , Watersheds -- South Africa -- Eastern Cape , Thematic maps -- South Africa -- Eastern Cape , Population density -- South Africa -- Eastern Cape , Mzimvubu River Watershed (South Africa) -- Census , Upper Tsitsa Catchment (South Africa)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/57296 , vital:26870
- Description: The purpose of this research was to determine if the integration of census and land cover data could provide evidence of spatial patterns and temporal change for the Upper Tsitsa Catchment. This thesis contributed to academic literature with regards to dasymetric mapping and provided a database for the Ntabelanga and Laleni Ecological Infrastructure Project. The study took place in the Upper Tsitsa Catchment which is located in the north-eastern region of the Eastern Cape and falls within the uMzimvubu Catchment. South Africa National Population Censuses for 2001 and 2011 and the National Land Cover data sets for 2000 and 2013/14 were used to create dasymetric maps depicting demographic changes over time for the catchment area. Spatial statistics were performed on the dasymetric and choropleth map to determine the accuracy of the data that was created. From the results, it was found that although the statistics were skewed, the method was more accurate in displaying the population densities, which was noted during the sampling process of the spatial analysis. It was found that there had been a decrease in the population density within the catchment. This affected the density of several other variables such as population race group, language and employment status statistics as decreases in these values could be due to individuals migrating out of the catchment as well as socio-economic upliftment, such as having better access to services. The use of dasymetric mapping allowed an accurate representation of the population density from the census data to be created. The results of the dasymetric mapping were more accurate as they depicted where the population within the enumeration areas were located, and recognised that some areas were populated while some areas were not. To conclude, it was found that using dasymetric mapping provided reliable and useful data about population density and enables comparison over time.
- Full Text:
- Date Issued: 2018
Development and optimisation of a novel Plasmodium falciparum Hsp90-Hop interaction assay
- Authors: Wambua, Lynn
- Date: 2018
- Subjects: Plasmodium falciparum , Molecular chaperones , Heat shock proteins , Protein-protein interactions , Antimalarials
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/62626 , vital:28216
- Description: Protein-protein interactions are involved in a range of disease processes and thus have become the focus of many drug discovery programs. Widespread drug resistance to all currently used antimalarial drugs drives the search for alternative drug targets with novel mechanisms of action that offer new therapeutic options. Molecular chaperones such as heat shock proteins facilitate protein folding, play a role in protein trafficking and prevent protein misfolding in cells under stress. Heat shock protein 90 (Hsp90) is a well-studied chaperone that has been the focus of cancer drug development with moderate success. In Plasmodium falciparum (P. falciparum), heat shock proteins are thought to play a vital role in parasite survival of the physiologically diverse habitats of the parasite lifecycle and because Hsp90 is prominently expressed in P. falciparum, the chaperone is considered a potentially ideal drug target. Hsp90 function in cells is regulated by interactions with co-chaperones, which includes Heat shock protein 70-Heat shock protein 90 organising protein (Hop). As opposed to directly inhibiting Hsp90 activity, targeting Hsp90 interaction with Hop has recently been suggested as an alternative method of Hsp90 inhibition that has not been explored in P. falciparum. The aim of this research project was to demonstrate PfHsp90 and PfHop robustly interact in vitro and to facilitate high-throughput screening of PfHsp90-PfHop inhibitors by developing and optimising a novel plate capture Hsp90-Hop interaction assay. To establish the assay, the respective domains of the proteins that mediate Hsp90-Hop interaction were used (Hsp90 C- terminal domain and Hop TPR2A domain). The human Hsp90 C-terminal domain and glutathione-S-transferase (GST) coding sequences were cloned into pET-28a(+) and murine and P. falciparum TPR2A sequences into pGEX-4T-1 plasmids to enable expression of histidine-tagged and GST fusion proteins, respectively, in Escherichia coli. The P. falciparum Hsp90 C-terminal domain sequence cloned into pET-28a(+) was supplied by GenScript. The constructs were transformed into T7 Express lysYcompetent E. coli cells and subsequent small- scale expression studies showed the recombinant proteins were expressed in a soluble form allowing for subsequent protein purification. Purification of the recombinant proteins was achieved using nickel-NTA and glutathione affinity chromatography for the His-tagged (Hsp90 C-terminal domains and GST) and GST fusion proteins (TPR2A domains), respectively. The purified proteins were used to establish and optimise mammalian and P. falciparum Hsp90- Hop interaction assays on nickel-coated plates by immobilising the His-tagged C-terminal domains on the plates and detecting the binding of the GST-TPR2A domains using a colorimetric GST enzyme assay. Z’-factor values above 0.5 were observed for both assays indicating good separation between the protein interaction signals and negative control background signals, although relatively high background signals were observed for the mammalian interaction due to non-specific binding of murine TPR2A to the plate. Designed human and P. falciparum TPR peptides were observed to be effective inhibitors of the mammalian and P. falciparum interactions, demonstrating the assay’s ability to respond to inhibitor compounds. Comparison of assay performance using GST assay kit reagents and lab- prepared reagents showed the assay was more efficient using lab-prepared reagents, however, lower GST signals were observed when comparing assay performance using a custom prepared Ni-NTA plate to a purchased Ni-NTA plate. The Hsp90-Hop interaction assays were also performed using an alternative assay format in which the GST-TPR2A fusion proteins were immobilised on glutathione-coated plates and binding of the His-tagged C-terminal domains detected with a nickel-horseradish peroxidase (HRP) conjugate and a colorimetric HRP substrate. The assay showed higher interaction signals for the P. falciparum proteins but comparatively low signals for the mammalian proteins. Z’-factor values for the assay were above 0.8 for both protein sets, suggesting this assay format is superior to the GST assay. However, further optimisation of this assay format is required. This study demonstrated direct binding of PfHsp90-PfHop in vitro and established a novel and robust PfHsp90-PfHop interaction assay format that can be used in future screening campaigns.
- Full Text:
- Date Issued: 2018
- Authors: Wambua, Lynn
- Date: 2018
- Subjects: Plasmodium falciparum , Molecular chaperones , Heat shock proteins , Protein-protein interactions , Antimalarials
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/62626 , vital:28216
- Description: Protein-protein interactions are involved in a range of disease processes and thus have become the focus of many drug discovery programs. Widespread drug resistance to all currently used antimalarial drugs drives the search for alternative drug targets with novel mechanisms of action that offer new therapeutic options. Molecular chaperones such as heat shock proteins facilitate protein folding, play a role in protein trafficking and prevent protein misfolding in cells under stress. Heat shock protein 90 (Hsp90) is a well-studied chaperone that has been the focus of cancer drug development with moderate success. In Plasmodium falciparum (P. falciparum), heat shock proteins are thought to play a vital role in parasite survival of the physiologically diverse habitats of the parasite lifecycle and because Hsp90 is prominently expressed in P. falciparum, the chaperone is considered a potentially ideal drug target. Hsp90 function in cells is regulated by interactions with co-chaperones, which includes Heat shock protein 70-Heat shock protein 90 organising protein (Hop). As opposed to directly inhibiting Hsp90 activity, targeting Hsp90 interaction with Hop has recently been suggested as an alternative method of Hsp90 inhibition that has not been explored in P. falciparum. The aim of this research project was to demonstrate PfHsp90 and PfHop robustly interact in vitro and to facilitate high-throughput screening of PfHsp90-PfHop inhibitors by developing and optimising a novel plate capture Hsp90-Hop interaction assay. To establish the assay, the respective domains of the proteins that mediate Hsp90-Hop interaction were used (Hsp90 C- terminal domain and Hop TPR2A domain). The human Hsp90 C-terminal domain and glutathione-S-transferase (GST) coding sequences were cloned into pET-28a(+) and murine and P. falciparum TPR2A sequences into pGEX-4T-1 plasmids to enable expression of histidine-tagged and GST fusion proteins, respectively, in Escherichia coli. The P. falciparum Hsp90 C-terminal domain sequence cloned into pET-28a(+) was supplied by GenScript. The constructs were transformed into T7 Express lysYcompetent E. coli cells and subsequent small- scale expression studies showed the recombinant proteins were expressed in a soluble form allowing for subsequent protein purification. Purification of the recombinant proteins was achieved using nickel-NTA and glutathione affinity chromatography for the His-tagged (Hsp90 C-terminal domains and GST) and GST fusion proteins (TPR2A domains), respectively. The purified proteins were used to establish and optimise mammalian and P. falciparum Hsp90- Hop interaction assays on nickel-coated plates by immobilising the His-tagged C-terminal domains on the plates and detecting the binding of the GST-TPR2A domains using a colorimetric GST enzyme assay. Z’-factor values above 0.5 were observed for both assays indicating good separation between the protein interaction signals and negative control background signals, although relatively high background signals were observed for the mammalian interaction due to non-specific binding of murine TPR2A to the plate. Designed human and P. falciparum TPR peptides were observed to be effective inhibitors of the mammalian and P. falciparum interactions, demonstrating the assay’s ability to respond to inhibitor compounds. Comparison of assay performance using GST assay kit reagents and lab- prepared reagents showed the assay was more efficient using lab-prepared reagents, however, lower GST signals were observed when comparing assay performance using a custom prepared Ni-NTA plate to a purchased Ni-NTA plate. The Hsp90-Hop interaction assays were also performed using an alternative assay format in which the GST-TPR2A fusion proteins were immobilised on glutathione-coated plates and binding of the His-tagged C-terminal domains detected with a nickel-horseradish peroxidase (HRP) conjugate and a colorimetric HRP substrate. The assay showed higher interaction signals for the P. falciparum proteins but comparatively low signals for the mammalian proteins. Z’-factor values for the assay were above 0.8 for both protein sets, suggesting this assay format is superior to the GST assay. However, further optimisation of this assay format is required. This study demonstrated direct binding of PfHsp90-PfHop in vitro and established a novel and robust PfHsp90-PfHop interaction assay format that can be used in future screening campaigns.
- Full Text:
- Date Issued: 2018
Assessing asset management in the Nelson Mandela Metropolitan Municipality
- Authors: Mahlangabeza, Bukeka
- Date: 2013
- Subjects: Asset management accounts -- South Africa -- Port Elizabeth , Local government -- South Africa -- Port Elizabeth , Strategic planning -- South Africa -- Port Elizabeth , Management -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:8848 , http://hdl.handle.net/10948/d1020108
- Description: Due to scarce financial resources, over-stretched budgets, pressures for service delivery and the dynamic nature of local government, asset management plays a key strategic role in enhancing local municipal functionality and efficiency. Asset management is the management of physical assets, infrastructure, and immoveable assets. It is an evolving discipline that is enhanced by the understanding of asset conditions and performance. It improves decision-making within local government. Every year, local government announces millions of Rands’ worth of assets that are unaccounted for and that go missing in annual reports. It was the purpose of this study to assess the asset management function at the Nelson Mandela Bay Municipality (NMBM). The aim is to improve the management of moveable assets by investigating the following variables: strategic management of assets, knowledge management, governance, leadership, the impact of corruption and skills management. The approach to the study is a qualitative research paradigm with a case study methodology and an interview as a research instrument. The sample chosen comprised people from the top level of municipal management down to municipal asset controllers. The two forms of data that were collected in the study were primary data and secondary data. Primary data was collected from municipal workers and collated together for analysis purposes. Secondary data was gathered from library sources such as journals, books, conference papers, government acts, municipal policies and local newspapers. This data informed the interview questions. The interview strategy was chosen as the best way to conduct the empirical part of this research and was guided by an interview guide. The study found that there are operational defects in the way asset management is organised. This makes the function seem unimportant. The results also indicated a lack of staff members and skills shortages; such as management and computer skills. Indications are that theft, negligence, corruption and carelessness negatively impact the management of assets. Recommendations include the filling of vacant posts, tightening of internal controls, providing the necessary training and improving the asset management. The study makes a contribution to the current body of knowledge.
- Full Text:
- Date Issued: 2013
- Authors: Mahlangabeza, Bukeka
- Date: 2013
- Subjects: Asset management accounts -- South Africa -- Port Elizabeth , Local government -- South Africa -- Port Elizabeth , Strategic planning -- South Africa -- Port Elizabeth , Management -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:8848 , http://hdl.handle.net/10948/d1020108
- Description: Due to scarce financial resources, over-stretched budgets, pressures for service delivery and the dynamic nature of local government, asset management plays a key strategic role in enhancing local municipal functionality and efficiency. Asset management is the management of physical assets, infrastructure, and immoveable assets. It is an evolving discipline that is enhanced by the understanding of asset conditions and performance. It improves decision-making within local government. Every year, local government announces millions of Rands’ worth of assets that are unaccounted for and that go missing in annual reports. It was the purpose of this study to assess the asset management function at the Nelson Mandela Bay Municipality (NMBM). The aim is to improve the management of moveable assets by investigating the following variables: strategic management of assets, knowledge management, governance, leadership, the impact of corruption and skills management. The approach to the study is a qualitative research paradigm with a case study methodology and an interview as a research instrument. The sample chosen comprised people from the top level of municipal management down to municipal asset controllers. The two forms of data that were collected in the study were primary data and secondary data. Primary data was collected from municipal workers and collated together for analysis purposes. Secondary data was gathered from library sources such as journals, books, conference papers, government acts, municipal policies and local newspapers. This data informed the interview questions. The interview strategy was chosen as the best way to conduct the empirical part of this research and was guided by an interview guide. The study found that there are operational defects in the way asset management is organised. This makes the function seem unimportant. The results also indicated a lack of staff members and skills shortages; such as management and computer skills. Indications are that theft, negligence, corruption and carelessness negatively impact the management of assets. Recommendations include the filling of vacant posts, tightening of internal controls, providing the necessary training and improving the asset management. The study makes a contribution to the current body of knowledge.
- Full Text:
- Date Issued: 2013
Investigating the relationship between the social phenomenon of Facebook and narcissistic socio-cultural tendencies
- Authors: Zdanow, Carla
- Date: 2011
- Subjects: Facebook (Electronic resource) , Narcissism , Self , Culture , Online social networks -- South Africa , Social media -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:8377 , http://hdl.handle.net/10948/1445 , Facebook (Electronic resource) , Narcissism , Self , Culture , Online social networks -- South Africa , Social media -- South Africa
- Description: Narcissism is increasingly being regarded as one of the biggest socio-cultural problems of the contemporary era. Indeed, recent studies by Baldwin and Stroman (2007) and Buffardi and Campbell (2008), among others, have advanced that new media technologies – in particular social networking websites – have significantly exacerbated the rise and spread of narcissism in contemporary society. Based on this premise, namely that social media provide the perfect platform for the promotion of self-infatuation, this research project will provide a critical analysis of the potential influence of social media in the development of a widespread narcissistic socio-cultural condition. In this regard, claims that increasingly consumerist, individualist and media-saturated societies are nurturing a culture of extreme narcissism, vanity and entitlement, will be examined in relation to an increase in the use of consumerorientated new media technologies. In particular, by examining the structural components of the popular social networking site, Facebook, this treatise will highlight the connection between the use of this form of new media and the engenderment of an acutely consumerist and narcissistic subjectivity – namely, commodity narcissism. That is, by examining the growth of narcissism from the 1940s through to the new millennium, the role of the media, and most recently new media technologies, in the promotion of commodity narcissism will be examined as factors of particular significance in the formation of contemporary subjectivity. In relation to this, the impact of commodity narcissism on the perpetuation and propagation of capitalist isolation, alienation and insecurity will be investigated with a view to exploring the potential impact of such narcissism on the efficacy of the democratic process. Finally, some remedial measures, which co-opt rather than negate such social media, will be proposed.
- Full Text:
- Date Issued: 2011
- Authors: Zdanow, Carla
- Date: 2011
- Subjects: Facebook (Electronic resource) , Narcissism , Self , Culture , Online social networks -- South Africa , Social media -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:8377 , http://hdl.handle.net/10948/1445 , Facebook (Electronic resource) , Narcissism , Self , Culture , Online social networks -- South Africa , Social media -- South Africa
- Description: Narcissism is increasingly being regarded as one of the biggest socio-cultural problems of the contemporary era. Indeed, recent studies by Baldwin and Stroman (2007) and Buffardi and Campbell (2008), among others, have advanced that new media technologies – in particular social networking websites – have significantly exacerbated the rise and spread of narcissism in contemporary society. Based on this premise, namely that social media provide the perfect platform for the promotion of self-infatuation, this research project will provide a critical analysis of the potential influence of social media in the development of a widespread narcissistic socio-cultural condition. In this regard, claims that increasingly consumerist, individualist and media-saturated societies are nurturing a culture of extreme narcissism, vanity and entitlement, will be examined in relation to an increase in the use of consumerorientated new media technologies. In particular, by examining the structural components of the popular social networking site, Facebook, this treatise will highlight the connection between the use of this form of new media and the engenderment of an acutely consumerist and narcissistic subjectivity – namely, commodity narcissism. That is, by examining the growth of narcissism from the 1940s through to the new millennium, the role of the media, and most recently new media technologies, in the promotion of commodity narcissism will be examined as factors of particular significance in the formation of contemporary subjectivity. In relation to this, the impact of commodity narcissism on the perpetuation and propagation of capitalist isolation, alienation and insecurity will be investigated with a view to exploring the potential impact of such narcissism on the efficacy of the democratic process. Finally, some remedial measures, which co-opt rather than negate such social media, will be proposed.
- Full Text:
- Date Issued: 2011
Opportunity and connectivity : selecting land managers for involvement in a conservation corridor linking two protected areas in the Langkloof Valley, South Africa
- Authors: McClure, Alice
- Date: 2011
- Subjects: Corridors (Ecology) -- South Africa -- Eastern Cape , National parks and reserves -- South Africa , Landscape ecology -- South Africa , Wildlife conservation -- South Africa , Conservation of natural resources -- South Africa , Land use -- South Africa -- Planning
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4770 , http://hdl.handle.net/10962/d1007590 , Corridors (Ecology) -- South Africa -- Eastern Cape , National parks and reserves -- South Africa , Landscape ecology -- South Africa , Wildlife conservation -- South Africa , Conservation of natural resources -- South Africa , Land use -- South Africa -- Planning
- Description: The Eden To Addo Corridor Initiative aims to connect formally protected areas in a conservation corridor from the coastal area of the Eden District near Plettenberg Bay, Western Cape, South Africa to the Addo National Elephant Park, Eastern Cape, South Africa. The corridor will incorporate government and privately owned land, and will be an attempt to maintain ecological processes at a range of spatial and temporal scales. The Langkloof Valley lies between the Baviaanskloof World Heritage Area and the Tstitsikamma National Park; two formally protected areas that will be incorporated into the Eden To Addo Corridor. Spatial prioritization analyses allow conservation planners to select areas that should be targeted for conservation action based on a range of criteria. Historically, ecological criteria have been included mostly alone in spatial prioritization. Recently, the idea of ‘conservation opportunity’ has emerged in the field of conservation planning; the notion suggests that a range of different types of data should be included in processes to spatially prioritise for conservation. By including those data defined as ‘human’ and ‘social’ data into prioritising activities, the feasibility of conservation plans can be accounted for, but historically conservation planners have failed to do so. I conducted a literature review that demonstrated that although the importance of human and social data are acknowledged in the conservation planning literature, these data that define opportunity are rarely actually included in spatial prioritisation analyses. I then carried out a social assessment that allowed me to define the social and human context of our study area and, specifically, what stewardship instruments land managers in the Langkloof would be prepared to engage. We found that land managers were generally willing to engage, but lacked the financial capacity to adopt conservation methods. Using a subset of the social and human data that were collected in the social assessment, I trialled a new Decision Support Software to fuse those data with ecological data in a novel attempt to identify priority areas for conservation action based on ecological integrity and feasibility. We also scheduled (ranked) land managers to approach for conservation action with a focus on local champions and clusters of land managers displaying strong conservation characteristics. Two corridors were identified; a major corridor in the western region of the valley and a secondary corridor closer to the middle. The members of the Initiative have been briefed on the outcomes, which provided them an opportunity to provide feedback; it is hoped that the framework of this study can be used for planning future connections. The Eden To Addo Corridor Initiative sent out a stewardship extension officer in February 2011 to approach those land managers areas that were identified. This planning exercise is a good demonstration of how, by collaborating effectively, academic conservation planners can contribute to supporting decision making by organizations that are implementing conservation action.
- Full Text:
- Date Issued: 2011
- Authors: McClure, Alice
- Date: 2011
- Subjects: Corridors (Ecology) -- South Africa -- Eastern Cape , National parks and reserves -- South Africa , Landscape ecology -- South Africa , Wildlife conservation -- South Africa , Conservation of natural resources -- South Africa , Land use -- South Africa -- Planning
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4770 , http://hdl.handle.net/10962/d1007590 , Corridors (Ecology) -- South Africa -- Eastern Cape , National parks and reserves -- South Africa , Landscape ecology -- South Africa , Wildlife conservation -- South Africa , Conservation of natural resources -- South Africa , Land use -- South Africa -- Planning
- Description: The Eden To Addo Corridor Initiative aims to connect formally protected areas in a conservation corridor from the coastal area of the Eden District near Plettenberg Bay, Western Cape, South Africa to the Addo National Elephant Park, Eastern Cape, South Africa. The corridor will incorporate government and privately owned land, and will be an attempt to maintain ecological processes at a range of spatial and temporal scales. The Langkloof Valley lies between the Baviaanskloof World Heritage Area and the Tstitsikamma National Park; two formally protected areas that will be incorporated into the Eden To Addo Corridor. Spatial prioritization analyses allow conservation planners to select areas that should be targeted for conservation action based on a range of criteria. Historically, ecological criteria have been included mostly alone in spatial prioritization. Recently, the idea of ‘conservation opportunity’ has emerged in the field of conservation planning; the notion suggests that a range of different types of data should be included in processes to spatially prioritise for conservation. By including those data defined as ‘human’ and ‘social’ data into prioritising activities, the feasibility of conservation plans can be accounted for, but historically conservation planners have failed to do so. I conducted a literature review that demonstrated that although the importance of human and social data are acknowledged in the conservation planning literature, these data that define opportunity are rarely actually included in spatial prioritisation analyses. I then carried out a social assessment that allowed me to define the social and human context of our study area and, specifically, what stewardship instruments land managers in the Langkloof would be prepared to engage. We found that land managers were generally willing to engage, but lacked the financial capacity to adopt conservation methods. Using a subset of the social and human data that were collected in the social assessment, I trialled a new Decision Support Software to fuse those data with ecological data in a novel attempt to identify priority areas for conservation action based on ecological integrity and feasibility. We also scheduled (ranked) land managers to approach for conservation action with a focus on local champions and clusters of land managers displaying strong conservation characteristics. Two corridors were identified; a major corridor in the western region of the valley and a secondary corridor closer to the middle. The members of the Initiative have been briefed on the outcomes, which provided them an opportunity to provide feedback; it is hoped that the framework of this study can be used for planning future connections. The Eden To Addo Corridor Initiative sent out a stewardship extension officer in February 2011 to approach those land managers areas that were identified. This planning exercise is a good demonstration of how, by collaborating effectively, academic conservation planners can contribute to supporting decision making by organizations that are implementing conservation action.
- Full Text:
- Date Issued: 2011
Precooling strategies for passenger vehicles
- Wachsmuth, Carsten Ulrich Harro
- Authors: Wachsmuth, Carsten Ulrich Harro
- Date: 2009
- Subjects: Automobiles -- Air conditioning , Air conditioning
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:9643 , http://hdl.handle.net/10948/930 , Automobiles -- Air conditioning , Air conditioning
- Description: By ventilating the vehicle’s interior during a soak, the peak cooling load is reduced and therefore the air-conditioning needs less power. This in turn translates to a smaller air-conditioning unit which would consume less power, be lighter and more compact. This solar driven ventilation of the vehicle’s interior during a soak is defined as precooling. During this project the best precooling strategy for passenger vehicles was found by investigating and testing different precooling strategies and evaluating them according to their thermodynamic performance, their potential implementation and their influence on the required cooling performance of the airconditioning cycle. The best performances were achieved by strategies with a high air flow rate and a relatively low air inlet temperature. Two categories of precooling strategies were examined: natural convection and forced convection strategies. Openings in the vehicle’s body that fit to all strategies had to be found; with a big potential concerning their thermodynamic performance and other aspects like prevention against water penetration. The best investigated precooling strategies proved to be the one which used the design outlet as an inlet and the opening of the HVAC as an outlet. With these openings a good air flow through the whole cabin can be guaranteed. In addition to that the strategy only uses existing openings of the body which prevent against water penetration. The required installation space for this strategy already exists and can be considered for the package of next generation vehicles.
- Full Text:
- Date Issued: 2009
- Authors: Wachsmuth, Carsten Ulrich Harro
- Date: 2009
- Subjects: Automobiles -- Air conditioning , Air conditioning
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:9643 , http://hdl.handle.net/10948/930 , Automobiles -- Air conditioning , Air conditioning
- Description: By ventilating the vehicle’s interior during a soak, the peak cooling load is reduced and therefore the air-conditioning needs less power. This in turn translates to a smaller air-conditioning unit which would consume less power, be lighter and more compact. This solar driven ventilation of the vehicle’s interior during a soak is defined as precooling. During this project the best precooling strategy for passenger vehicles was found by investigating and testing different precooling strategies and evaluating them according to their thermodynamic performance, their potential implementation and their influence on the required cooling performance of the airconditioning cycle. The best performances were achieved by strategies with a high air flow rate and a relatively low air inlet temperature. Two categories of precooling strategies were examined: natural convection and forced convection strategies. Openings in the vehicle’s body that fit to all strategies had to be found; with a big potential concerning their thermodynamic performance and other aspects like prevention against water penetration. The best investigated precooling strategies proved to be the one which used the design outlet as an inlet and the opening of the HVAC as an outlet. With these openings a good air flow through the whole cabin can be guaranteed. In addition to that the strategy only uses existing openings of the body which prevent against water penetration. The required installation space for this strategy already exists and can be considered for the package of next generation vehicles.
- Full Text:
- Date Issued: 2009
The challenge of applying systematic conservation planning to the marine environment using expert knowledge (focus: Sparidae)
- Authors: Bause, Mariëtte
- Date: 2004
- Subjects: Marine parks and reserves -- South Africa , Nature conservation -- South Africa , Fishery conservation -- South Africa , Sparidae
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11064 , http://hdl.handle.net/10948/343 , Marine parks and reserves -- South Africa , Nature conservation -- South Africa , Fishery conservation -- South Africa , Sparidae
- Description: Although the marine environment has generally been considered to be less in need of attention from conservationists, politicians and managers than the terrestrial environment, there has been an increasing appreciation that there is little left in the marine environment that can be considered unaltered by humans. Conservation action is therefore urgently needed to protect marine populations, species and environments from anthropogenic pressures. Since overharvesting seems to be the main cause of the collapse in coastal ecosystems, areas need to be protected so that stocks can recover. However, the proclamation of such marine protected areas (MPAs) need to be well planned to ensure that species are well represented and that their biological requirements are met in the long term. While terrestrial conservation planners have started to incorporate biological, social and economic information into so-called systematic conservation plans, marine planners have been more hesitant to use these approaches due to the greater openness of the ocean (with the associated greater dispersal of marine organisms and propogules, lower levels of endemism, and lack of ownership) and the paucity of available information. The current study aimed to investigate the applicability of systematic approaches to the process of selecting sites for a network of potential replenishment zones for seabream species that could supplement protection already afforded by existing no-fishing reserves. Since many of these seabream species are popular targets in both the recreational and commercial linefishery sectors, they have been overexploited and are in need of additional protection. Information on these species and the diversity of their associated reefs were obtained through consultations with scientists, managers and resource-users (namely SCUBA divers, spearfishers as well as recreational and commercial fishers). These data were obtained during workshops where Participatory Rural Appraisal (PRA) scoring and mapping methods were applied. Although ordinal-scale, rather than interval-or ratio-scale data were obtained, these data could be used in an interactive selection process. Because these methods require relatively cheap materials and few personnel, they could be used to collect information where limited budgets are available. Quantitative targets for the protection of individual species were set on the basis of their status in the Marine Living Resource Act, since this reflected their different requirements for protection. A preliminary investigation indicated that the current no-fishing reserves effectively achieved most of these goals for seabream species. The selection of sites for the replenishment network was based on irreplaceability analyses, while decisions between sites with lower irreplaceability values were based on explicit design rules. A total of 26 sites were required to achieve targets for all species. These replenishment zones could potentially be established, managed and monitored by voluntary community groups. This study therefore indicated that systematic conservation planning can successfully be applied to the marine environment, while the involvement of stakeholders is crucial to ensure that conservation action is supported.
- Full Text:
- Date Issued: 2004
- Authors: Bause, Mariëtte
- Date: 2004
- Subjects: Marine parks and reserves -- South Africa , Nature conservation -- South Africa , Fishery conservation -- South Africa , Sparidae
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11064 , http://hdl.handle.net/10948/343 , Marine parks and reserves -- South Africa , Nature conservation -- South Africa , Fishery conservation -- South Africa , Sparidae
- Description: Although the marine environment has generally been considered to be less in need of attention from conservationists, politicians and managers than the terrestrial environment, there has been an increasing appreciation that there is little left in the marine environment that can be considered unaltered by humans. Conservation action is therefore urgently needed to protect marine populations, species and environments from anthropogenic pressures. Since overharvesting seems to be the main cause of the collapse in coastal ecosystems, areas need to be protected so that stocks can recover. However, the proclamation of such marine protected areas (MPAs) need to be well planned to ensure that species are well represented and that their biological requirements are met in the long term. While terrestrial conservation planners have started to incorporate biological, social and economic information into so-called systematic conservation plans, marine planners have been more hesitant to use these approaches due to the greater openness of the ocean (with the associated greater dispersal of marine organisms and propogules, lower levels of endemism, and lack of ownership) and the paucity of available information. The current study aimed to investigate the applicability of systematic approaches to the process of selecting sites for a network of potential replenishment zones for seabream species that could supplement protection already afforded by existing no-fishing reserves. Since many of these seabream species are popular targets in both the recreational and commercial linefishery sectors, they have been overexploited and are in need of additional protection. Information on these species and the diversity of their associated reefs were obtained through consultations with scientists, managers and resource-users (namely SCUBA divers, spearfishers as well as recreational and commercial fishers). These data were obtained during workshops where Participatory Rural Appraisal (PRA) scoring and mapping methods were applied. Although ordinal-scale, rather than interval-or ratio-scale data were obtained, these data could be used in an interactive selection process. Because these methods require relatively cheap materials and few personnel, they could be used to collect information where limited budgets are available. Quantitative targets for the protection of individual species were set on the basis of their status in the Marine Living Resource Act, since this reflected their different requirements for protection. A preliminary investigation indicated that the current no-fishing reserves effectively achieved most of these goals for seabream species. The selection of sites for the replenishment network was based on irreplaceability analyses, while decisions between sites with lower irreplaceability values were based on explicit design rules. A total of 26 sites were required to achieve targets for all species. These replenishment zones could potentially be established, managed and monitored by voluntary community groups. This study therefore indicated that systematic conservation planning can successfully be applied to the marine environment, while the involvement of stakeholders is crucial to ensure that conservation action is supported.
- Full Text:
- Date Issued: 2004
An investigation into the occurrence of major abscess causing bacteria at the law meat exporting abattoir in the Northern Cape province of South Africa
- Authors: Delport, Riëtte
- Date: 2003
- Subjects: Abscess , Meat -- Microbiology , Slaughtering and slaughter-houses -- South Africa -- Northern Cape
- Language: English
- Type: Thesis , Masters , MTech (Agricultural Management)
- Identifier: vital:10971 , http://hdl.handle.net/10948/249 , Abscess , Meat -- Microbiology , Slaughtering and slaughter-houses -- South Africa -- Northern Cape
- Description: The main objectives of this study can be divided into four categories: a) The determination of the presence of abscesses in carcasses. b) The determination whether the abscesses are area bound. c) The determination of the causative bacteria which is responsible for abscess formation. d) The determination of the economic impact due to carcass rejections and trimmings. The research area consists of the biggest part of the Northern Cape province, which can further be divided into three main production areas, namely Groblershoop, Gordonia and Hantam. The Groblershoop area includes Groblershoop and the surrounding districts. The Gordonia area includes Upington and the Kalahari. The Hantam area includes Calvinia, Springbok and the other districts bordering the areas. All the carcasses were slaughtered at an export abattoir, namely LAW, which is situated in Groblershoop. The Upington veterinary laboratory bacteriologically cultured the abscess material and the results were processed and noted. The following results were prominent: a) It was determined that abscesses did occur in carcasses. b) It was determined that in the Hantam main production area the prevalence of abscesses is the highest, although the slaughter numbers are the lowest. c) It was determined that the bacterium that was responsible for most of the abscesses was Escherichia coli. d) It was determined that the total occurrence of bacteria did not have a significant economic impact, because there were not many carcass rejections due to abscesses. The economic impact, however, of the value of the total rejections, might become a problem.
- Full Text:
- Date Issued: 2003
- Authors: Delport, Riëtte
- Date: 2003
- Subjects: Abscess , Meat -- Microbiology , Slaughtering and slaughter-houses -- South Africa -- Northern Cape
- Language: English
- Type: Thesis , Masters , MTech (Agricultural Management)
- Identifier: vital:10971 , http://hdl.handle.net/10948/249 , Abscess , Meat -- Microbiology , Slaughtering and slaughter-houses -- South Africa -- Northern Cape
- Description: The main objectives of this study can be divided into four categories: a) The determination of the presence of abscesses in carcasses. b) The determination whether the abscesses are area bound. c) The determination of the causative bacteria which is responsible for abscess formation. d) The determination of the economic impact due to carcass rejections and trimmings. The research area consists of the biggest part of the Northern Cape province, which can further be divided into three main production areas, namely Groblershoop, Gordonia and Hantam. The Groblershoop area includes Groblershoop and the surrounding districts. The Gordonia area includes Upington and the Kalahari. The Hantam area includes Calvinia, Springbok and the other districts bordering the areas. All the carcasses were slaughtered at an export abattoir, namely LAW, which is situated in Groblershoop. The Upington veterinary laboratory bacteriologically cultured the abscess material and the results were processed and noted. The following results were prominent: a) It was determined that abscesses did occur in carcasses. b) It was determined that in the Hantam main production area the prevalence of abscesses is the highest, although the slaughter numbers are the lowest. c) It was determined that the bacterium that was responsible for most of the abscesses was Escherichia coli. d) It was determined that the total occurrence of bacteria did not have a significant economic impact, because there were not many carcass rejections due to abscesses. The economic impact, however, of the value of the total rejections, might become a problem.
- Full Text:
- Date Issued: 2003
The garden as a metaphor for paradise
- Authors: Adlard, Michelle Catherine
- Date: 2001
- Subjects: Paradise in art , Garden of paradise , Gardens -- Design -- Early works to 1800
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2391 , http://hdl.handle.net/10962/d1002187 , Paradise in art , Garden of paradise , Gardens -- Design -- Early works to 1800
- Description: In this half thesis the use of the garden as a metaphor for paradise has been explored. The English word “ paradise“ was derived from the Greek word “ paradeisos” which in turn was derived from the Old Avestana “ pairi-daeza,” meaning an enclosure. In Ancient Persia the concept applied to an enclosed garden in the modern sense of the word. For this reason the thesis begins with an examination of the development of the garden in this desert region. A more-or-less continuous chain of development in both the physical and allegorical nature of the garden is traced through history from these Ancient Persian beginnings to the height of Mughal architecture (epitomised by the Taj Mahal), by way of the Muslim expansion through Central Asia and Europe. While the core elements of garden design were set in Ancient Persian times, and recur throughout the period studied, the impact of Islam on the local Persian culture brought about a new development of allegorical meaning associated with the garden. This allegorical development reached its apex, too, in the Taj Mahal in which, it is argued, the metaphorical representation of paradise in the garden tomb was made astonishingly explicit. The research for this mini thesis was gathered from secondary sources, including many published books and academic papers, photographic and diagrammatic evidence of extant ancient gardens, and reproductions of carpet designs.
- Full Text:
- Date Issued: 2001
- Authors: Adlard, Michelle Catherine
- Date: 2001
- Subjects: Paradise in art , Garden of paradise , Gardens -- Design -- Early works to 1800
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2391 , http://hdl.handle.net/10962/d1002187 , Paradise in art , Garden of paradise , Gardens -- Design -- Early works to 1800
- Description: In this half thesis the use of the garden as a metaphor for paradise has been explored. The English word “ paradise“ was derived from the Greek word “ paradeisos” which in turn was derived from the Old Avestana “ pairi-daeza,” meaning an enclosure. In Ancient Persia the concept applied to an enclosed garden in the modern sense of the word. For this reason the thesis begins with an examination of the development of the garden in this desert region. A more-or-less continuous chain of development in both the physical and allegorical nature of the garden is traced through history from these Ancient Persian beginnings to the height of Mughal architecture (epitomised by the Taj Mahal), by way of the Muslim expansion through Central Asia and Europe. While the core elements of garden design were set in Ancient Persian times, and recur throughout the period studied, the impact of Islam on the local Persian culture brought about a new development of allegorical meaning associated with the garden. This allegorical development reached its apex, too, in the Taj Mahal in which, it is argued, the metaphorical representation of paradise in the garden tomb was made astonishingly explicit. The research for this mini thesis was gathered from secondary sources, including many published books and academic papers, photographic and diagrammatic evidence of extant ancient gardens, and reproductions of carpet designs.
- Full Text:
- Date Issued: 2001
The economic rationale and modalities for rural infrastructure development: developmental local government in rural service delivery
- Stilwell, Ted, Atkinson, Doreen
- Authors: Stilwell, Ted , Atkinson, Doreen
- Date: 1999-09
- Subjects: Rural development -- South Africa , South Africa -- Economic conditions , Infrastructure (Economics) -- South Africa , Sustainable development -- South Africa , Local government -- South Africa
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/73841 , vital:30234 , 1919692444
- Description: The Policy Unit of the Development Bank of Southern Africa (DBSA) has developed a number of strategic themes embracing many cross-cutting issues. The main thrust of the Unit’s work, however, focuses on the core mandate of the Bank - infrastructure. In order to provide for a coherent whole, rural infrastructure must fit into a broader policy framework. Globally, the goals set for the first two decades of the next millennium are to address poverty and achieve food security. The DBSA can contribute to South Africa’s position on these global themes by addressing the issue of rural infrastructure delivery, taking cognisance of the South African government’s Growth, Employment and Redistribution (GEAR) programme. This discussion document will specifically address the local economic development activities of farm production and rural livelihoods in order to achieve food security, address poverty and foster economic growth in the marginalised, infrastructure-deprived rural areas of the country. It is assumed that additional entrepreneurial economic opportunities will arise at village and town level as producers become nett surplus producers, eg in small grain milling, cottage industry, village markets, processing (value adding), etc. In contrast to urban development, where economic activity is assumed by investing in infrastructure, the DBSA will actively have to support the transformation of economic activity in the rural scenario. One of the past successes of the DBSA has been the introduction of the Farmer Support Programme (FSP). The FSP’s provision of services and support, based on the needs of existing smallholders, is embedded in participatory planning and action. Without local initiatives, rural infrastructure cannot be delivered effectively to bolster economic growth, create jobs and redistribute income. Most of the country’s poor live in rural areas, and without rural development there can be no GEAR. Life in these areas needs to be made liveable by encouraging entrepreneurial development, which includes facilitating agriculture, creating jobs and increasing rural income through appropriate government actions for improving the welfare of rural households. One of the ways in which national, provincial and local government could have a broad-based impact is by providing rural economic infrastructure and, to this end, local government, including regional and district councils, has been mandated to develop integrated development plans. These plans have to include economic development plans, and entrepreneurial development has been singled out as important in this regard. From this it follows that district councils and municipalities have a prime responsibility for developing entrepreneurs, including farmers in their rural constituency.
- Full Text:
- Date Issued: 1999-09
- Authors: Stilwell, Ted , Atkinson, Doreen
- Date: 1999-09
- Subjects: Rural development -- South Africa , South Africa -- Economic conditions , Infrastructure (Economics) -- South Africa , Sustainable development -- South Africa , Local government -- South Africa
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/73841 , vital:30234 , 1919692444
- Description: The Policy Unit of the Development Bank of Southern Africa (DBSA) has developed a number of strategic themes embracing many cross-cutting issues. The main thrust of the Unit’s work, however, focuses on the core mandate of the Bank - infrastructure. In order to provide for a coherent whole, rural infrastructure must fit into a broader policy framework. Globally, the goals set for the first two decades of the next millennium are to address poverty and achieve food security. The DBSA can contribute to South Africa’s position on these global themes by addressing the issue of rural infrastructure delivery, taking cognisance of the South African government’s Growth, Employment and Redistribution (GEAR) programme. This discussion document will specifically address the local economic development activities of farm production and rural livelihoods in order to achieve food security, address poverty and foster economic growth in the marginalised, infrastructure-deprived rural areas of the country. It is assumed that additional entrepreneurial economic opportunities will arise at village and town level as producers become nett surplus producers, eg in small grain milling, cottage industry, village markets, processing (value adding), etc. In contrast to urban development, where economic activity is assumed by investing in infrastructure, the DBSA will actively have to support the transformation of economic activity in the rural scenario. One of the past successes of the DBSA has been the introduction of the Farmer Support Programme (FSP). The FSP’s provision of services and support, based on the needs of existing smallholders, is embedded in participatory planning and action. Without local initiatives, rural infrastructure cannot be delivered effectively to bolster economic growth, create jobs and redistribute income. Most of the country’s poor live in rural areas, and without rural development there can be no GEAR. Life in these areas needs to be made liveable by encouraging entrepreneurial development, which includes facilitating agriculture, creating jobs and increasing rural income through appropriate government actions for improving the welfare of rural households. One of the ways in which national, provincial and local government could have a broad-based impact is by providing rural economic infrastructure and, to this end, local government, including regional and district councils, has been mandated to develop integrated development plans. These plans have to include economic development plans, and entrepreneurial development has been singled out as important in this regard. From this it follows that district councils and municipalities have a prime responsibility for developing entrepreneurs, including farmers in their rural constituency.
- Full Text:
- Date Issued: 1999-09
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