Diversity of bacteria isolated from the flies Musca domestica (Muscidae) andChrysomya megacephala (Calliphoridae) with emphasis on vectored pathogens
- Brits, Devon, Brooks, Margot, Villet, Martin H
- Authors: Brits, Devon , Brooks, Margot , Villet, Martin H
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66904 , vital:28998 , ISSN 1021-3589 , https://doi.org/10.4001/003.024.0365
- Description: Publisher version , We evaluated the bacteria occurring externally on Musca domestica and Chrysomya megacephala, the two most common synanthropic flies which may be found at many refuse sites throughout the world. Bacteria cultured from 10 specimens of each species were isolated, Gram-stained and examined microscopically, and divided into morphologically distinct ‘pseudospecies', to avoid excessive duplication of genetic identification. About 350 bp of the 16S ribosomalRNAgene was amplified from genomicDNAextracted from each ‘pseudospecies', sequenced, and bacteria identified using BLASTn. Nineteen different types of colony were identified from M. domestica, with Pseudomonas sp. and Swine Manure Bacterium SP14 being most abundant. Chrysomya megacephala yielded 15 distinct pseudospecies with total colony counts approximating to 10 000 from 10 plates, where 80 % of colonies were non-pathogenic Bacillus pumilus. A total of 18 species were identified genetically: three shared by the fly species; four unique to C. megacephala, and 13 unique to M. domestica. Half of these 18 species were pathogenic, two or three others were food spoilers and the rest were environmental or commensal bacteria from soil or plant matter. This study added three new pathogenic strains of bacteria and one new environmental strain to the list of bacteria reported to be vectored by these flies.
- Full Text: false
- Date Issued: 2016
- Authors: Brits, Devon , Brooks, Margot , Villet, Martin H
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/66904 , vital:28998 , ISSN 1021-3589 , https://doi.org/10.4001/003.024.0365
- Description: Publisher version , We evaluated the bacteria occurring externally on Musca domestica and Chrysomya megacephala, the two most common synanthropic flies which may be found at many refuse sites throughout the world. Bacteria cultured from 10 specimens of each species were isolated, Gram-stained and examined microscopically, and divided into morphologically distinct ‘pseudospecies', to avoid excessive duplication of genetic identification. About 350 bp of the 16S ribosomalRNAgene was amplified from genomicDNAextracted from each ‘pseudospecies', sequenced, and bacteria identified using BLASTn. Nineteen different types of colony were identified from M. domestica, with Pseudomonas sp. and Swine Manure Bacterium SP14 being most abundant. Chrysomya megacephala yielded 15 distinct pseudospecies with total colony counts approximating to 10 000 from 10 plates, where 80 % of colonies were non-pathogenic Bacillus pumilus. A total of 18 species were identified genetically: three shared by the fly species; four unique to C. megacephala, and 13 unique to M. domestica. Half of these 18 species were pathogenic, two or three others were food spoilers and the rest were environmental or commensal bacteria from soil or plant matter. This study added three new pathogenic strains of bacteria and one new environmental strain to the list of bacteria reported to be vectored by these flies.
- Full Text: false
- Date Issued: 2016
Oral tradition and the creation of late prehistory in Roviana Lagoon, Solomon Islands
- Sheppard, Peter, Walter, Richard, Aswani, Shankar
- Authors: Sheppard, Peter , Walter, Richard , Aswani, Shankar
- Date: 2004
- Subjects: To be catalogued
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/439958 , vital:73723 , ISBN 0975047639
- Description: The use of oral tradition or oral history in archaeology is often a contentious issue. In this paper we briefly review methodological issues surrounding the use of such data and follow this with a case study using our research into the last 1,000 years of prehistory in Roviana Lagoon (New Georgia Group, Solomon Islands). We argue that it is not possible to generalize cross-culturally about the historicity of oral tradition/history. However, in the Roviana case, careful use of ethnohistory and archaeology together indicates that:(a) Roviana oral history is linear;(b) there is a close relationship between genealogical age and radiocarbon age; and (c) the modern uses of the oral tradition by Roviana provide a theory of their use in the past. We conclude that the model for the formation of the Roviana Chiefdom which emerges from the working back and forth between archaeology and ethnohistory has much more explanatory power than one based on either source of data by itself.
- Full Text:
- Date Issued: 2004
- Authors: Sheppard, Peter , Walter, Richard , Aswani, Shankar
- Date: 2004
- Subjects: To be catalogued
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/439958 , vital:73723 , ISBN 0975047639
- Description: The use of oral tradition or oral history in archaeology is often a contentious issue. In this paper we briefly review methodological issues surrounding the use of such data and follow this with a case study using our research into the last 1,000 years of prehistory in Roviana Lagoon (New Georgia Group, Solomon Islands). We argue that it is not possible to generalize cross-culturally about the historicity of oral tradition/history. However, in the Roviana case, careful use of ethnohistory and archaeology together indicates that:(a) Roviana oral history is linear;(b) there is a close relationship between genealogical age and radiocarbon age; and (c) the modern uses of the oral tradition by Roviana provide a theory of their use in the past. We conclude that the model for the formation of the Roviana Chiefdom which emerges from the working back and forth between archaeology and ethnohistory has much more explanatory power than one based on either source of data by itself.
- Full Text:
- Date Issued: 2004
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