Biological properties and interactions of Kalaharituber pfeilii
- Authors: Krele, Viwe
- Date: 2019
- Subjects: Kalaharituber pfeilii , Pezizales -- South Africa , Desert plants -- South Africa , Truffle culture -- South Africa , Plant biochemical genetics , Enzymes -- Analysis
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/72257 , vital:30022
- Description: Dessert truffles are seasonal macro fungi and have been identified in several parts of the world including South Africa. The first part of the present study dealt with the assessment of the biologically active compounds of the Kalahari truffles found in the Northern Cape of South Africa. Truffles extracts (methanol, ethanol, aqueous) were investigated for their antimicrobial properties towards Gram-positive and Gram-negative bacteria. The results demonstrated that the truffle extracts tested had no inhibitory effects against the bacterial isolates. The truffle mycelial growth was also noted to be ineffective against the selected bacteria. The bacteria tested in the present study showed some antagonistic effects against the fungus. Cultures of K. pfeilii were also screened for enzyme production including amylase, protease, cellulose, and laccase. Evaluation of the potential of K. pfeilii mycelia to produce these industrially and economically important enzymes demonstrated both amylase and protease activity. However, for laccase and cellulose, no activity was detected. The second part of the present study aimed at optimizing biomass production by K. pfeilii in liquid culture media. FF Microplate containing 95 discreet carbon sources were employed to test for substrate utilization. Blanked readings above 0.1 were regarded as positive for utilization, and 4 substrates were selected as potential substrates and were included in liquid media. Media was evaluated for mycelial biomass production. Of the carbon sources tested sucrose proved to be the most suitable for supporting mycelial growth. The third part of the current study included investigating the diversity of microbial communities colonizing the rhizosheath of Stipagrostis ciliata var. capensis (the host plant of K. pfeilii) and these were identified by means of next-generation sequencing using Illumina Miseq. Bioinformatics tools were utilized in analyzing the data. Actinobacteria were found to be the most dominant bacterial phylum, followed by unclassified bacteria, Proteobacteria, and Acidobacteria. The top 25 sequences were selected and clustered into bacterial OTUs (at 97% threshold) which were assigned into 1 phylum (Actinobacteria), 1 family (Geodermatophilaceae) and 23 genera. This phylum is well known for its secondary metabolites. Streptomyces sp. was the most frequently encountered genus. The results from this study necessitate further investigations with regards to the function and evolution of fungal-bacterial associations. Wheather these bacteria have a contribution towards the truffle development, it is still not confirmed.
- Full Text:
- Authors: Krele, Viwe
- Date: 2019
- Subjects: Kalaharituber pfeilii , Pezizales -- South Africa , Desert plants -- South Africa , Truffle culture -- South Africa , Plant biochemical genetics , Enzymes -- Analysis
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/72257 , vital:30022
- Description: Dessert truffles are seasonal macro fungi and have been identified in several parts of the world including South Africa. The first part of the present study dealt with the assessment of the biologically active compounds of the Kalahari truffles found in the Northern Cape of South Africa. Truffles extracts (methanol, ethanol, aqueous) were investigated for their antimicrobial properties towards Gram-positive and Gram-negative bacteria. The results demonstrated that the truffle extracts tested had no inhibitory effects against the bacterial isolates. The truffle mycelial growth was also noted to be ineffective against the selected bacteria. The bacteria tested in the present study showed some antagonistic effects against the fungus. Cultures of K. pfeilii were also screened for enzyme production including amylase, protease, cellulose, and laccase. Evaluation of the potential of K. pfeilii mycelia to produce these industrially and economically important enzymes demonstrated both amylase and protease activity. However, for laccase and cellulose, no activity was detected. The second part of the present study aimed at optimizing biomass production by K. pfeilii in liquid culture media. FF Microplate containing 95 discreet carbon sources were employed to test for substrate utilization. Blanked readings above 0.1 were regarded as positive for utilization, and 4 substrates were selected as potential substrates and were included in liquid media. Media was evaluated for mycelial biomass production. Of the carbon sources tested sucrose proved to be the most suitable for supporting mycelial growth. The third part of the current study included investigating the diversity of microbial communities colonizing the rhizosheath of Stipagrostis ciliata var. capensis (the host plant of K. pfeilii) and these were identified by means of next-generation sequencing using Illumina Miseq. Bioinformatics tools were utilized in analyzing the data. Actinobacteria were found to be the most dominant bacterial phylum, followed by unclassified bacteria, Proteobacteria, and Acidobacteria. The top 25 sequences were selected and clustered into bacterial OTUs (at 97% threshold) which were assigned into 1 phylum (Actinobacteria), 1 family (Geodermatophilaceae) and 23 genera. This phylum is well known for its secondary metabolites. Streptomyces sp. was the most frequently encountered genus. The results from this study necessitate further investigations with regards to the function and evolution of fungal-bacterial associations. Wheather these bacteria have a contribution towards the truffle development, it is still not confirmed.
- Full Text:
“New ways of telling”: African textual forms and dissemination in the age of digital media
- Authors: Friedemann, Oriole Megan
- Date: 2019
- Subjects: Digital media -- Africa , Self-publishing -- Africa , African literature , Literature publishing -- Technological innovations , Blog authorship -- Africa , African Storybook Reader , FunDza Literacy Project , Long Story SHORT
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/115105 , vital:34078
- Description: In the age of digital media, creators are using the versatile nature of information and communication technologies and the ubiquity of the web to publish and distribute texts, circumventing traditional gatekeepers such as publishing institutions. In Africa, where web access and digitisation are relatively new, storytellers are eagerly exploring new mediums and the possibilities that they provide for African narratives and African representation. This thesis looks at the digital platforms of the African Storybook Reader, the FunDza Literacy Project, and Long Story SHORT, as well as Dudu Busani-Dube’s novel Hlomu the Wife, which first gained popularity on a blog platform. It examines three different web series, An African City, The Foxy Five, and Tuko Macho, as well as a transmedia documentary, Love Radio. The texts are grouped into literatures disseminated from digital platforms, localised narratives that explore the urban African woman, and narratives that make use of participatory culture. These are texts that make use of digital tools and platforms to create and disseminate African stories, making diverse and indigenous narratives more easily accessible to both local and global audiences. This thesis argues that digitisation and the global nature of the internet have created opportunities for Africans to become producers and exporters of indigenous information and representation, rather than passive consumers of imported knowledge, or subjects of external characterisation.
- Full Text:
- Authors: Friedemann, Oriole Megan
- Date: 2019
- Subjects: Digital media -- Africa , Self-publishing -- Africa , African literature , Literature publishing -- Technological innovations , Blog authorship -- Africa , African Storybook Reader , FunDza Literacy Project , Long Story SHORT
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/115105 , vital:34078
- Description: In the age of digital media, creators are using the versatile nature of information and communication technologies and the ubiquity of the web to publish and distribute texts, circumventing traditional gatekeepers such as publishing institutions. In Africa, where web access and digitisation are relatively new, storytellers are eagerly exploring new mediums and the possibilities that they provide for African narratives and African representation. This thesis looks at the digital platforms of the African Storybook Reader, the FunDza Literacy Project, and Long Story SHORT, as well as Dudu Busani-Dube’s novel Hlomu the Wife, which first gained popularity on a blog platform. It examines three different web series, An African City, The Foxy Five, and Tuko Macho, as well as a transmedia documentary, Love Radio. The texts are grouped into literatures disseminated from digital platforms, localised narratives that explore the urban African woman, and narratives that make use of participatory culture. These are texts that make use of digital tools and platforms to create and disseminate African stories, making diverse and indigenous narratives more easily accessible to both local and global audiences. This thesis argues that digitisation and the global nature of the internet have created opportunities for Africans to become producers and exporters of indigenous information and representation, rather than passive consumers of imported knowledge, or subjects of external characterisation.
- Full Text:
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