Spectroscopic and nonlinear optical characterisation of alpha substituted binuclear phthalocyanines
- Authors: Ngubeni, Grace Nomthandazo
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/5038 , vital:20757
- Full Text:
- Authors: Ngubeni, Grace Nomthandazo
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/5038 , vital:20757
- Full Text:
Synthesis and bioassay of rationally designed DXR inhibitors as potential antimalarial lead compounds
- Authors: Nokalipa, Iviwe Cwaita
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4888 , vital:20740
- Description: Globally, the eradication of malaria has been challenging due to the problem of resistance that past and currently available drugs exhibit. This is exacerbated by the inherent need for anti-malarial drugs to be affordable to the poverty-stricken majority that is primarily affected by this burden. This research has focused on the development of potential inhibitors of 1-deoxy-D- xylulose-5 phosphate reductoisomerase (DXR), an essential enzyme in the mevalonate- independent pathway for the biosynthesis of isoprenoids in Plasmodium falciparum. DXR mediates the isomerisation and reduction of 1-deoxy-D-xylulose-5-phosphate into 2-C- methyl-D-erithrytol 4-phosphate. This enzyme has been determined to be a target for the development of novel antimalarial agents and extensive molecular modelling has been undertaken to develop inhibitors that fit into the DXR active site. The in silico docking data have been used to inform the design and synthesis of various N-benzyl-substituted phosphoramidate ligands that were determined to have potential as novel substrate mimics of fosmidomycin, a known DXR inhibitor. Synthesis of the N-benzyl-substituted phosphoramidate ligands involved a nine-step sequence commencing from diethyl phosphoramidate. In all, some 40 compounds have been prepared, some of them new, and were fully characterized using NMR. Attention has also been given to the mass spectrometric fragmentation patterns exhibited by selected intermediates. Four of the final products were evaluated for in vitro antimalarial activity using a PLDH assay and exhibited IC50 values < 100 µM.
- Full Text:
- Authors: Nokalipa, Iviwe Cwaita
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4888 , vital:20740
- Description: Globally, the eradication of malaria has been challenging due to the problem of resistance that past and currently available drugs exhibit. This is exacerbated by the inherent need for anti-malarial drugs to be affordable to the poverty-stricken majority that is primarily affected by this burden. This research has focused on the development of potential inhibitors of 1-deoxy-D- xylulose-5 phosphate reductoisomerase (DXR), an essential enzyme in the mevalonate- independent pathway for the biosynthesis of isoprenoids in Plasmodium falciparum. DXR mediates the isomerisation and reduction of 1-deoxy-D-xylulose-5-phosphate into 2-C- methyl-D-erithrytol 4-phosphate. This enzyme has been determined to be a target for the development of novel antimalarial agents and extensive molecular modelling has been undertaken to develop inhibitors that fit into the DXR active site. The in silico docking data have been used to inform the design and synthesis of various N-benzyl-substituted phosphoramidate ligands that were determined to have potential as novel substrate mimics of fosmidomycin, a known DXR inhibitor. Synthesis of the N-benzyl-substituted phosphoramidate ligands involved a nine-step sequence commencing from diethyl phosphoramidate. In all, some 40 compounds have been prepared, some of them new, and were fully characterized using NMR. Attention has also been given to the mass spectrometric fragmentation patterns exhibited by selected intermediates. Four of the final products were evaluated for in vitro antimalarial activity using a PLDH assay and exhibited IC50 values < 100 µM.
- Full Text:
The independent high rate algal pond as a unit operation in tertiary wastewater treatment
- Authors: Clark, Stewart James
- Date: 2002
- Subjects: Algae -- Biotechnology , Sewage -- Purification -- Biological treatment
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4092 , http://hdl.handle.net/10962/d1007805
- Description: The development of the High Rate Algal Pond (HRAP) as an independent tertiary treatment unit operation for phosphate and nitrate removal is reported. A novel Integrated Algal Ponding System (lAPS) design is proposed for nutrient removal from the effluents of both a conventional domestic sewage treatment plant and from an Advanced Integrated Wastewater Ponding System (AIWPS). The viability of an independently operated HRAP has been identified and termed the Independent High Rate Algal Pond (l-HRAP). A 500 m² pilot 1- HRAP was operated in such a way as to facilitate the precipitation of calcium phosphate, known to be controlled by pH (greater than 9.4) and resulting in final phosphate levels of less than 1 mg.L⁻¹ as P0₄-P. The incorporation of the I-HRAP into a denitrification process was also investigated. Continuously fed column reactors, utilising algal biomass as a carbon source, showed that the heterotrophic bacterial community dominant in the anaerobic algal sludge were denitrifying the nitrate in the feed. It was demonstrated that as the cultures were stressed (using increased nitrate concentrations, anaerobiosis and light starvation) total polysaccharide (TPS) concentrations increased, with a notable increase 111 the exopolysaccharide (EPS) fraction. These experiments corroborated the hypothesis that harvested microalgal biomass can be manipulated to produce, and release, exopolymeric substances under stress conditions, and which may serve as carbon source for denitrification. In both batch flask studies and in laboratory-scale reactor systems, harvested microalgal biomass from an HRAP was shown to produce exopolymeric substances under stress conditions. Initial high loading-rates of greater than 20 mg.L⁻¹ NO₃-N resulted in double the amount of exopolysaccharide production than in flasks with initial low loading-rates (less than 5 mg.L⁻¹ NO₃-N). Making use of an upflow anaerobic sludge blanket-type degrading-bed reactor, and an anaerobic, flooded trickle filter (ANTRIC) receiving HRAP effluent, the relationship between denitrification and the changes in polysaccharide content was investigated. This phenomenon has considerable beneficial implications in biological wastewater treatment systems where high nitrate concentration in the final effluent is a potential mitigating factor. Identification of the heterotrophic bacteria active in the denitrification process was attempted. This study presents a first report on the development and operation of the I-HRAP and has been followed by a technical-scale pilot plant evaluation of the process in the tertiary treatment of domestic wastewaters.
- Full Text:
- Authors: Clark, Stewart James
- Date: 2002
- Subjects: Algae -- Biotechnology , Sewage -- Purification -- Biological treatment
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4092 , http://hdl.handle.net/10962/d1007805
- Description: The development of the High Rate Algal Pond (HRAP) as an independent tertiary treatment unit operation for phosphate and nitrate removal is reported. A novel Integrated Algal Ponding System (lAPS) design is proposed for nutrient removal from the effluents of both a conventional domestic sewage treatment plant and from an Advanced Integrated Wastewater Ponding System (AIWPS). The viability of an independently operated HRAP has been identified and termed the Independent High Rate Algal Pond (l-HRAP). A 500 m² pilot 1- HRAP was operated in such a way as to facilitate the precipitation of calcium phosphate, known to be controlled by pH (greater than 9.4) and resulting in final phosphate levels of less than 1 mg.L⁻¹ as P0₄-P. The incorporation of the I-HRAP into a denitrification process was also investigated. Continuously fed column reactors, utilising algal biomass as a carbon source, showed that the heterotrophic bacterial community dominant in the anaerobic algal sludge were denitrifying the nitrate in the feed. It was demonstrated that as the cultures were stressed (using increased nitrate concentrations, anaerobiosis and light starvation) total polysaccharide (TPS) concentrations increased, with a notable increase 111 the exopolysaccharide (EPS) fraction. These experiments corroborated the hypothesis that harvested microalgal biomass can be manipulated to produce, and release, exopolymeric substances under stress conditions, and which may serve as carbon source for denitrification. In both batch flask studies and in laboratory-scale reactor systems, harvested microalgal biomass from an HRAP was shown to produce exopolymeric substances under stress conditions. Initial high loading-rates of greater than 20 mg.L⁻¹ NO₃-N resulted in double the amount of exopolysaccharide production than in flasks with initial low loading-rates (less than 5 mg.L⁻¹ NO₃-N). Making use of an upflow anaerobic sludge blanket-type degrading-bed reactor, and an anaerobic, flooded trickle filter (ANTRIC) receiving HRAP effluent, the relationship between denitrification and the changes in polysaccharide content was investigated. This phenomenon has considerable beneficial implications in biological wastewater treatment systems where high nitrate concentration in the final effluent is a potential mitigating factor. Identification of the heterotrophic bacteria active in the denitrification process was attempted. This study presents a first report on the development and operation of the I-HRAP and has been followed by a technical-scale pilot plant evaluation of the process in the tertiary treatment of domestic wastewaters.
- Full Text:
Aspects of the ecology and reproductive biology of the limpet, Helcion pruinosus (Gastropoda : prosobranchia)
- Authors: Henninger, Tony Oskar
- Date: 2000
- Subjects: Prosobranchia , Limpets -- Reproduction , Limpets -- Ecology , Prosobranchia -- Ecology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5641 , http://hdl.handle.net/10962/d1005323 , Prosobranchia , Limpets -- Reproduction , Limpets -- Ecology , Prosobranchia -- Ecology
- Description: Helcion pruinosus, the rayed limpet, is found in the midshore region of the intertidal zone, (i.e. the Balanoid zone) of rocky shores, from Saldanha Bay to Sodwana Bay along the coast of South Africa. At Gonubie (32°57'S/25°01'E) this species of limpet was found under boulders, (when not feeding), and could reach average densities of 85/ m². The limpets prefer smooth boulders, avoiding those encrusted by coralline algae or boulders with a rough texture. There was no habitat segregation between adult and juvenile H. pruinosus, i.e. there was no sign of up-shore migration by larger individuals. Males out-numbered females by, on average 1.4 : 1, which was a similar sex ratio to that of the congeneric species, H. pectunculus (Gray, 996). Unlike H. pectunculus, there were no differences in shell lengths between males and females (p = 0.946 at Gonubie, south-east coast and p = 0.961 at Kommetjie, south-west coast; t-test). Shell height could also not be used as a criterion to differentiate between the sexes. The average maximum shell length of H. pruinosus at Gonubie and Kommetjie was 25 mm, but the maximum shell lengths found were 30 mm, (one individual in each case), on both the southeast coast and west coast. Growth occurred most rapidly in the first year of life, with individuals reaching 15 mm after 1 year. In the second year limpet growth slowed to only 7 mm. Sexual maturity was reached at a shell length of between 11 to 14 mm (at an age of 8 months to 1 year). The life-span of H. pruinosus was determined at 2.55 years on the south-east coast and 2.9 years on the south-west coast. Micro-growth bands were produced tidally. Growth occurred allometrically, i.e. shell length increased at a faster rate than shell height. The limpets foraged during low tide, at night, presumably feeding on epilithic algae on the boulders. A second smaller peak of limpet activity was often recorded during the day, when low tide coincided with dawn). More limpets (up to ten times more) were active at spring tides compared to neap tides. Limpet activity was greatest during the spring tides of autumn. During all seasons limpet activity peaked 30 minutes before low water, after which numbers decreased rapidly. All limpets had retreated beneath the boulders before being covered by the flooding tide. It was concluded that the limpets were responding to both endogenous and exogenous cues, but the actual stimulus for retreat could not be determined. The limpets did not return to a fixed scar nor did they return rigidly to a home site. H. pruinosus was found to be gonochoristic. Sexes could be separated, on dissection, according to the colour of their gonads (males had white gonads, whilst that of the females was olive-green). Gonads were present throughout the year in most individuals, i.e. totally spent animals were never observed. Reproductive seasonality was similar in west coast and south-east coast animals. The gonad indices of the west coast limpets were higher, (maximum of 35%), compared to that on the southeast coast, (25%). The animals were probably trickle spawners, with some individuals liberating gametes throughout the year. There was however synchrony in gametogenesis between male and female limpets on both the west coast and south-east coast lines. Gonad indices peaked in the summer months (October to December) with a second minor peak in April/May. These peaks occurred before the release of the gametes in spawning events. The gonad index was lowest in winter, (July). The ovaries were full of mature eggs (oocytes having a diameter of 200 to 250 ~) prior to spawning. The acinal wall thickness had been decreasing prior to spawning. After spawning there was an increase in previtellogenic eggs « 100 μm) in females, and an increase in the acinal wall thickness to indicate spermatogenesis. The two populations are subjected to different environmental conditions and the actual cue for stimulating the release of gametes could not be determined. Finally, a table of differences between H pruinosus and its sister species, H pectunculus was created to show the life-style of H pruinosus.
- Full Text:
- Authors: Henninger, Tony Oskar
- Date: 2000
- Subjects: Prosobranchia , Limpets -- Reproduction , Limpets -- Ecology , Prosobranchia -- Ecology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5641 , http://hdl.handle.net/10962/d1005323 , Prosobranchia , Limpets -- Reproduction , Limpets -- Ecology , Prosobranchia -- Ecology
- Description: Helcion pruinosus, the rayed limpet, is found in the midshore region of the intertidal zone, (i.e. the Balanoid zone) of rocky shores, from Saldanha Bay to Sodwana Bay along the coast of South Africa. At Gonubie (32°57'S/25°01'E) this species of limpet was found under boulders, (when not feeding), and could reach average densities of 85/ m². The limpets prefer smooth boulders, avoiding those encrusted by coralline algae or boulders with a rough texture. There was no habitat segregation between adult and juvenile H. pruinosus, i.e. there was no sign of up-shore migration by larger individuals. Males out-numbered females by, on average 1.4 : 1, which was a similar sex ratio to that of the congeneric species, H. pectunculus (Gray, 996). Unlike H. pectunculus, there were no differences in shell lengths between males and females (p = 0.946 at Gonubie, south-east coast and p = 0.961 at Kommetjie, south-west coast; t-test). Shell height could also not be used as a criterion to differentiate between the sexes. The average maximum shell length of H. pruinosus at Gonubie and Kommetjie was 25 mm, but the maximum shell lengths found were 30 mm, (one individual in each case), on both the southeast coast and west coast. Growth occurred most rapidly in the first year of life, with individuals reaching 15 mm after 1 year. In the second year limpet growth slowed to only 7 mm. Sexual maturity was reached at a shell length of between 11 to 14 mm (at an age of 8 months to 1 year). The life-span of H. pruinosus was determined at 2.55 years on the south-east coast and 2.9 years on the south-west coast. Micro-growth bands were produced tidally. Growth occurred allometrically, i.e. shell length increased at a faster rate than shell height. The limpets foraged during low tide, at night, presumably feeding on epilithic algae on the boulders. A second smaller peak of limpet activity was often recorded during the day, when low tide coincided with dawn). More limpets (up to ten times more) were active at spring tides compared to neap tides. Limpet activity was greatest during the spring tides of autumn. During all seasons limpet activity peaked 30 minutes before low water, after which numbers decreased rapidly. All limpets had retreated beneath the boulders before being covered by the flooding tide. It was concluded that the limpets were responding to both endogenous and exogenous cues, but the actual stimulus for retreat could not be determined. The limpets did not return to a fixed scar nor did they return rigidly to a home site. H. pruinosus was found to be gonochoristic. Sexes could be separated, on dissection, according to the colour of their gonads (males had white gonads, whilst that of the females was olive-green). Gonads were present throughout the year in most individuals, i.e. totally spent animals were never observed. Reproductive seasonality was similar in west coast and south-east coast animals. The gonad indices of the west coast limpets were higher, (maximum of 35%), compared to that on the southeast coast, (25%). The animals were probably trickle spawners, with some individuals liberating gametes throughout the year. There was however synchrony in gametogenesis between male and female limpets on both the west coast and south-east coast lines. Gonad indices peaked in the summer months (October to December) with a second minor peak in April/May. These peaks occurred before the release of the gametes in spawning events. The gonad index was lowest in winter, (July). The ovaries were full of mature eggs (oocytes having a diameter of 200 to 250 ~) prior to spawning. The acinal wall thickness had been decreasing prior to spawning. After spawning there was an increase in previtellogenic eggs « 100 μm) in females, and an increase in the acinal wall thickness to indicate spermatogenesis. The two populations are subjected to different environmental conditions and the actual cue for stimulating the release of gametes could not be determined. Finally, a table of differences between H pruinosus and its sister species, H pectunculus was created to show the life-style of H pruinosus.
- Full Text:
Environmental and socio-economic effects associated with the planting of Atriplex nummularia Lindl. (Oldman saltbush) in the Karoo
- Authors: Hobson, Colin Desmond
- Date: 1991
- Subjects: Botany, Economic Plant introduction -- South Africa -- Karoo Plants, Cultivated
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: vital:4794 , http://hdl.handle.net/10962/d1001894
- Description: This study examines the environmental and socio-economic effects associated with the planting of Atriplex nummularia Lindl. (Oldman saltbush) in the karoo. This is the first study of its kind on oldman saltbush and serves as an important scoping exercise in assessing the impact of saltbush on its surrounds and in identifying additional aspects which require investigation. The environmental parameters investigated include microclimatic effects, soil induced changes, salinity effects on the germination of selected karoo species and compositional changes in the indigenous plant community within a saltbush plantation. When compared to indigenous karoo plant species oldman saltbush is found to ameliorate soil temperatures and increase surface soil salinity (specifically sodium chloride), on an individual plant basis. Salinity is shown to have a differential effect on the germination of selected karoo shrubs. The indigenous plant community within a forty year old saltbush plantation is impoverished with both density of individuals and species richness being markedly reduced. Saltbush is identified as an unsuitable nurse plant for the majority of karoo shrubs. Farm size in relation to both actual and intended area established to saltbush is considered. The area of saltbush already established in the karoo is considered too insignificant to have any wide socio-economic impact. Farmers who intend planting large areas of their farms to saltbush are in the minority. It is those farmers who already have some saltbush planted, who intend planting more. Ecologically based management guidelines are presented, suggesting practices which minimize the detrimental effects of saltbush on soil salinity and the indigenous plant community. These guidelines are practical and in keeping with the overall objectives of optimum resource utilisation and the national grazing strategy for South Africa.
- Full Text:
- Authors: Hobson, Colin Desmond
- Date: 1991
- Subjects: Botany, Economic Plant introduction -- South Africa -- Karoo Plants, Cultivated
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: vital:4794 , http://hdl.handle.net/10962/d1001894
- Description: This study examines the environmental and socio-economic effects associated with the planting of Atriplex nummularia Lindl. (Oldman saltbush) in the karoo. This is the first study of its kind on oldman saltbush and serves as an important scoping exercise in assessing the impact of saltbush on its surrounds and in identifying additional aspects which require investigation. The environmental parameters investigated include microclimatic effects, soil induced changes, salinity effects on the germination of selected karoo species and compositional changes in the indigenous plant community within a saltbush plantation. When compared to indigenous karoo plant species oldman saltbush is found to ameliorate soil temperatures and increase surface soil salinity (specifically sodium chloride), on an individual plant basis. Salinity is shown to have a differential effect on the germination of selected karoo shrubs. The indigenous plant community within a forty year old saltbush plantation is impoverished with both density of individuals and species richness being markedly reduced. Saltbush is identified as an unsuitable nurse plant for the majority of karoo shrubs. Farm size in relation to both actual and intended area established to saltbush is considered. The area of saltbush already established in the karoo is considered too insignificant to have any wide socio-economic impact. Farmers who intend planting large areas of their farms to saltbush are in the minority. It is those farmers who already have some saltbush planted, who intend planting more. Ecologically based management guidelines are presented, suggesting practices which minimize the detrimental effects of saltbush on soil salinity and the indigenous plant community. These guidelines are practical and in keeping with the overall objectives of optimum resource utilisation and the national grazing strategy for South Africa.
- Full Text:
Environmental and socio-economic effects associated with the planting of Atriplex nummularia Lindl. (Oldman saltbush) in the Karoo
- Authors: Hobson, Colin Desmond
- Date: 1991
- Subjects: Botany, Economic Plant introduction -- South Africa -- Karoo Plants, Cultivated
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: vital:4175 , http://hdl.handle.net/10962/d1002023
- Description: This study examines the environmental and socio-economic effects associated with the planting of Atriplex nummularia Lindl. (Oldman saltbush) in the karoo. This is the first study of its kind on oldman saltbush and serves as an important scoping exercise in assessing the impact of saltbush on its surrounds and in identifying additional aspects which require investigation. The environmental parameters investigated include microclimatic effects, soil induced changes, salinity effects on the germination of selected karoo species and compositional changes in the indigenous plant community within a saltbush plantation. When compared to indigenous karoo plant species oldman saltbush is found to ameliorate soil temperatures and increase surface soil salinity (specifically sodium chloride), on an individual plant basis. Salinity is shown to have a differential effect on the germination of selected karoo shrubs. The indigenous plant community within a forty year old saltbush plantation is impoverished with both density of individuals and species richness being markedly reduced. Saltbush is identified as an unsuitable nurse plant for the majority of karoo shrubs. Farm size in relation to both actual and intended area established to saltbush is considered. The area of saltbush already established in the karoo is considered too insignificant to have any wide socio-economic impact. Farmers who intend planting large areas of their farms to saltbush are in the minority. It is those farmers who already have some saltbush planted, who intend planting more. Ecologically based management guidelines are presented, suggesting practices which minimize the detrimental effects of saltbush on soil salinity and the indigenous plant community. These guidelines are practical and in keeping with the overall objectives of optimum resource utilisation and the national grazing strategy for South Africa.
- Full Text:
- Authors: Hobson, Colin Desmond
- Date: 1991
- Subjects: Botany, Economic Plant introduction -- South Africa -- Karoo Plants, Cultivated
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: vital:4175 , http://hdl.handle.net/10962/d1002023
- Description: This study examines the environmental and socio-economic effects associated with the planting of Atriplex nummularia Lindl. (Oldman saltbush) in the karoo. This is the first study of its kind on oldman saltbush and serves as an important scoping exercise in assessing the impact of saltbush on its surrounds and in identifying additional aspects which require investigation. The environmental parameters investigated include microclimatic effects, soil induced changes, salinity effects on the germination of selected karoo species and compositional changes in the indigenous plant community within a saltbush plantation. When compared to indigenous karoo plant species oldman saltbush is found to ameliorate soil temperatures and increase surface soil salinity (specifically sodium chloride), on an individual plant basis. Salinity is shown to have a differential effect on the germination of selected karoo shrubs. The indigenous plant community within a forty year old saltbush plantation is impoverished with both density of individuals and species richness being markedly reduced. Saltbush is identified as an unsuitable nurse plant for the majority of karoo shrubs. Farm size in relation to both actual and intended area established to saltbush is considered. The area of saltbush already established in the karoo is considered too insignificant to have any wide socio-economic impact. Farmers who intend planting large areas of their farms to saltbush are in the minority. It is those farmers who already have some saltbush planted, who intend planting more. Ecologically based management guidelines are presented, suggesting practices which minimize the detrimental effects of saltbush on soil salinity and the indigenous plant community. These guidelines are practical and in keeping with the overall objectives of optimum resource utilisation and the national grazing strategy for South Africa.
- Full Text:
The pineal gland as a model to elucidate the primary mode of action of sympathoactive agents
- Authors: Welman, Alan David
- Date: 1991
- Subjects: Pineal gland , Cythochemistry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3876 , http://hdl.handle.net/10962/d1001610
- Description: An attempt was made to use the pineal gland as a model for the study of the primary mode of action of sympathoactive agents. Two drugs were investigated, viz. alpha-methyldopa and ephedrine whose mode of action is not entirely clear. Organ cultures of pineal glands from rats treated chronically with alpha-methyldopa showed enhanced conversion of radioactive serotonin to melatonin (aMT) , as well as its precursor Nacetylserotonin (aHT). This treatment was also found to raise Nacetyltransferase (NAT) activity. These increases associated with alpha-methyldopa treatment were further enhanced by the beta-adrenergic agonist, isoproterenol, suggesting a supersensitivity-type effect occurring at the level of the beta-receptor. A subsequent binding study, however, showed a decrease in beta-receptor binding with exposure to alpha-methyldopa, providing mitigating evidence against the occurrence of a supersensitivity phenomenon. It is possible that a metabolite of alpha-methyldopa acts as an alpha 1 and beta-adrenergic agonist, resulting in greater melatonin (aMT) and N-acetylserotonin (aHT) synthesis than by a beta-adrenergic agonist, isoproterenol. Combined treatment of pineals with alpha-methyldopa and an alphareceptor blocker, phentolamine, resulted in melatonin (aMT) , Nacetylserotonin (aHT) , and N-acetyltransferase (NAT) activity levels which were lower than those obtained with alpha-methyldopa treatment alone, thus confirming the alpha-adrenergic activity of the metabolite of alpha-methyldopa. Additional pineal metabolites were isolated and measured simultaneously in the organ culture experiments. Organ cultures of rat pineal glands treated with ephedrine showed raised levels of melatonin (aMT) and N-acetylserotonin (aHT). Treatment with ephedrine also produced raised N-acetyltransferase activity. A further enhancement of these parameters was induced by norepinephrine, suggesting a supersensitivity-type effect occurring at the level of the beta-adrenergic receptor. Rats were treated with reserpine (a norepinephrine depleter) and the pineals exposed to ephedrine. Endogenous norepinephrine normally released by the action of ephedrine was thus absent, and under these conditions, levels of melatonin (aMT) and N-acetylserotonin (aHT) were reduced. N-acetyltransferase (NAT) activity was also reduced, but maintained levels pointing to substantial adrenergic activity of ephedrine as well as norepinephrine released by virtue of the drug's action. A subsequent binding study showed a decrease in beta-adrenergic receptor binding with exposure to ephedrine and a further decrease in ephedrine treated pineals from reserpine treated rats, thus ruling out the occurrence of a supersensitivity phenomenon. It is possible that both ephedrine and released norepinephrine have alpha- and beta-receptor activity. Additional pineal metabolites were isolated and measured in the organ culture experiments. A 16-hour time profile of the production of melatonin (aMT) and N-acetylserotonin (aHT) with norepinephrine and ephedrine treatment provided useful information regarding the course of action of the two agents. A pineal cell-culture system was developed and exposed to ephedrine and norepinephrine. N-acetyltransferase (NAT) activity levels measured after exposure to these agents were raised, confirming the adrenergic activity of both in the model. Finally, an HPLC system coupled to a UV detector was used in an attempt to measure melatonin (aMT) extracted from pineal organ culture media. The results showed that melatonin could be measured by this method, however, a more sensitive detection system was recommended for future work.
- Full Text:
- Authors: Welman, Alan David
- Date: 1991
- Subjects: Pineal gland , Cythochemistry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3876 , http://hdl.handle.net/10962/d1001610
- Description: An attempt was made to use the pineal gland as a model for the study of the primary mode of action of sympathoactive agents. Two drugs were investigated, viz. alpha-methyldopa and ephedrine whose mode of action is not entirely clear. Organ cultures of pineal glands from rats treated chronically with alpha-methyldopa showed enhanced conversion of radioactive serotonin to melatonin (aMT) , as well as its precursor Nacetylserotonin (aHT). This treatment was also found to raise Nacetyltransferase (NAT) activity. These increases associated with alpha-methyldopa treatment were further enhanced by the beta-adrenergic agonist, isoproterenol, suggesting a supersensitivity-type effect occurring at the level of the beta-receptor. A subsequent binding study, however, showed a decrease in beta-receptor binding with exposure to alpha-methyldopa, providing mitigating evidence against the occurrence of a supersensitivity phenomenon. It is possible that a metabolite of alpha-methyldopa acts as an alpha 1 and beta-adrenergic agonist, resulting in greater melatonin (aMT) and N-acetylserotonin (aHT) synthesis than by a beta-adrenergic agonist, isoproterenol. Combined treatment of pineals with alpha-methyldopa and an alphareceptor blocker, phentolamine, resulted in melatonin (aMT) , Nacetylserotonin (aHT) , and N-acetyltransferase (NAT) activity levels which were lower than those obtained with alpha-methyldopa treatment alone, thus confirming the alpha-adrenergic activity of the metabolite of alpha-methyldopa. Additional pineal metabolites were isolated and measured simultaneously in the organ culture experiments. Organ cultures of rat pineal glands treated with ephedrine showed raised levels of melatonin (aMT) and N-acetylserotonin (aHT). Treatment with ephedrine also produced raised N-acetyltransferase activity. A further enhancement of these parameters was induced by norepinephrine, suggesting a supersensitivity-type effect occurring at the level of the beta-adrenergic receptor. Rats were treated with reserpine (a norepinephrine depleter) and the pineals exposed to ephedrine. Endogenous norepinephrine normally released by the action of ephedrine was thus absent, and under these conditions, levels of melatonin (aMT) and N-acetylserotonin (aHT) were reduced. N-acetyltransferase (NAT) activity was also reduced, but maintained levels pointing to substantial adrenergic activity of ephedrine as well as norepinephrine released by virtue of the drug's action. A subsequent binding study showed a decrease in beta-adrenergic receptor binding with exposure to ephedrine and a further decrease in ephedrine treated pineals from reserpine treated rats, thus ruling out the occurrence of a supersensitivity phenomenon. It is possible that both ephedrine and released norepinephrine have alpha- and beta-receptor activity. Additional pineal metabolites were isolated and measured in the organ culture experiments. A 16-hour time profile of the production of melatonin (aMT) and N-acetylserotonin (aHT) with norepinephrine and ephedrine treatment provided useful information regarding the course of action of the two agents. A pineal cell-culture system was developed and exposed to ephedrine and norepinephrine. N-acetyltransferase (NAT) activity levels measured after exposure to these agents were raised, confirming the adrenergic activity of both in the model. Finally, an HPLC system coupled to a UV detector was used in an attempt to measure melatonin (aMT) extracted from pineal organ culture media. The results showed that melatonin could be measured by this method, however, a more sensitive detection system was recommended for future work.
- Full Text:
The pineal gland as a model to elucidate the primary mode of action of sympathoactive agents
- Authors: Welman, Alan David
- Date: 1991
- Subjects: Pineal gland , Cythochemistry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4279 , http://hdl.handle.net/10962/d1002005 , Pineal gland
- Description: An attempt was made to use the pineal gland as a model for the study of the primary mode of action of sympathoactive agents. Two drugs were investigated, viz. alpha-methyldopa and ephedrine whose mode of action is not entirely clear. Organ cultures of pineal glands from rats treated chronically with alpha-methyldopa showed enhanced conversion of radioactive serotonin to melatonin (aMT) , as well as its precursor Nacetylserotonin (aHT). This treatment was also found to raise Nacetyltransferase (NAT) activity. These increases associated with alpha-methyldopa treatment were further enhanced by the beta-adrenergic agonist, isoproterenol, suggesting a supersensitivity-type effect occurring at the level of the beta-receptor. A subsequent binding study, however, showed a decrease in beta-receptor binding with exposure to alpha-methyldopa, providing mitigating evidence against the occurrence of a supersensitivity phenomenon. It is possible that a metabolite of alpha-methyldopa acts as an alpha 1 and beta-adrenergic agonist, resulting in greater melatonin (aMT) and N-acetylserotonin (aHT) synthesis than by a beta-adrenergic agonist, isoproterenol. Combined treatment of pineals with alpha-methyldopa and an alphareceptor blocker, phentolamine, resulted in melatonin (aMT) , Nacetylserotonin (aHT) , and N-acetyltransferase (NAT) activity levels which were lower than those obtained with alpha-methyldopa treatment alone, thus confirming the alpha-adrenergic activity of the metabolite of alpha-methyldopa. Additional pineal metabolites were isolated and measured simultaneously in the organ culture experiments. Organ cultures of rat pineal glands treated with ephedrine showed raised levels of melatonin (aMT) and N-acetylserotonin (aHT). Treatment with ephedrine also produced raised N-acetyltransferase activity. A further enhancement of these parameters was induced by norepinephrine, suggesting a supersensitivity-type effect occurring at the level of the beta-adrenergic receptor. Rats were treated with reserpine (a norepinephrine depleter) and the pineals exposed to ephedrine. Endogenous norepinephrine normally released by the action of ephedrine was thus absent, and under these conditions, levels of melatonin (aMT) and N-acetylserotonin (aHT) were reduced. N-acetyltransferase (NAT) activity was also reduced, but maintained levels pointing to substantial adrenergic activity of ephedrine as well as norepinephrine released by virtue of the drug's action. A subsequent binding study showed a decrease in beta-adrenergic receptor binding with exposure to ephedrine and a further decrease in ephedrine treated pineals from reserpine treated rats, thus ruling out the occurrence of a supersensitivity phenomenon. It is possible that both ephedrine and released norepinephrine have alpha- and beta-receptor activity. Additional pineal metabolites were isolated and measured in the organ culture experiments. A 16-hour time profile of the production of melatonin (aMT) and N-acetylserotonin (aHT) with norepinephrine and ephedrine treatment provided useful information regarding the course of action of the two agents. A pineal cell-culture system was developed and exposed to ephedrine and norepinephrine. N-acetyltransferase (NAT) activity levels measured after exposure to these agents were raised, confirming the adrenergic activity of both in the model. Finally, an HPLC system coupled to a UV detector was used in an attempt to measure melatonin (aMT) extracted from pineal organ culture media. The results showed that melatonin could be measured by this method, however, a more sensitive detection system was recommended for future work
- Full Text:
- Authors: Welman, Alan David
- Date: 1991
- Subjects: Pineal gland , Cythochemistry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4279 , http://hdl.handle.net/10962/d1002005 , Pineal gland
- Description: An attempt was made to use the pineal gland as a model for the study of the primary mode of action of sympathoactive agents. Two drugs were investigated, viz. alpha-methyldopa and ephedrine whose mode of action is not entirely clear. Organ cultures of pineal glands from rats treated chronically with alpha-methyldopa showed enhanced conversion of radioactive serotonin to melatonin (aMT) , as well as its precursor Nacetylserotonin (aHT). This treatment was also found to raise Nacetyltransferase (NAT) activity. These increases associated with alpha-methyldopa treatment were further enhanced by the beta-adrenergic agonist, isoproterenol, suggesting a supersensitivity-type effect occurring at the level of the beta-receptor. A subsequent binding study, however, showed a decrease in beta-receptor binding with exposure to alpha-methyldopa, providing mitigating evidence against the occurrence of a supersensitivity phenomenon. It is possible that a metabolite of alpha-methyldopa acts as an alpha 1 and beta-adrenergic agonist, resulting in greater melatonin (aMT) and N-acetylserotonin (aHT) synthesis than by a beta-adrenergic agonist, isoproterenol. Combined treatment of pineals with alpha-methyldopa and an alphareceptor blocker, phentolamine, resulted in melatonin (aMT) , Nacetylserotonin (aHT) , and N-acetyltransferase (NAT) activity levels which were lower than those obtained with alpha-methyldopa treatment alone, thus confirming the alpha-adrenergic activity of the metabolite of alpha-methyldopa. Additional pineal metabolites were isolated and measured simultaneously in the organ culture experiments. Organ cultures of rat pineal glands treated with ephedrine showed raised levels of melatonin (aMT) and N-acetylserotonin (aHT). Treatment with ephedrine also produced raised N-acetyltransferase activity. A further enhancement of these parameters was induced by norepinephrine, suggesting a supersensitivity-type effect occurring at the level of the beta-adrenergic receptor. Rats were treated with reserpine (a norepinephrine depleter) and the pineals exposed to ephedrine. Endogenous norepinephrine normally released by the action of ephedrine was thus absent, and under these conditions, levels of melatonin (aMT) and N-acetylserotonin (aHT) were reduced. N-acetyltransferase (NAT) activity was also reduced, but maintained levels pointing to substantial adrenergic activity of ephedrine as well as norepinephrine released by virtue of the drug's action. A subsequent binding study showed a decrease in beta-adrenergic receptor binding with exposure to ephedrine and a further decrease in ephedrine treated pineals from reserpine treated rats, thus ruling out the occurrence of a supersensitivity phenomenon. It is possible that both ephedrine and released norepinephrine have alpha- and beta-receptor activity. Additional pineal metabolites were isolated and measured in the organ culture experiments. A 16-hour time profile of the production of melatonin (aMT) and N-acetylserotonin (aHT) with norepinephrine and ephedrine treatment provided useful information regarding the course of action of the two agents. A pineal cell-culture system was developed and exposed to ephedrine and norepinephrine. N-acetyltransferase (NAT) activity levels measured after exposure to these agents were raised, confirming the adrenergic activity of both in the model. Finally, an HPLC system coupled to a UV detector was used in an attempt to measure melatonin (aMT) extracted from pineal organ culture media. The results showed that melatonin could be measured by this method, however, a more sensitive detection system was recommended for future work
- Full Text:
A critical study of the curcumin method for the determination of boron in plant material
- Authors: Davidson, Raymond John
- Date: 1958
- Subjects: Plants -- Nutrition , Boron , Turmeric
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4458 , http://hdl.handle.net/10962/d1011003
- Description: Methods for the determination of boron are reviewed. A thorough investigation of the curcumin method was undertaken. Factors such as spectral absorption, boron volatilisation and the effects of pH, the stability of reagents and colour solutions, the drying of reaction products and the interference from certain elements, were investigated. A study of the efficiency of extracting boron from plant material using an ashing procedure is presented. The modified curcumin method for the determination of boron in plant material is described. The statistical accuracy and precision of the modified method is presented. The modified method was used to determine the boron content in citrus and pineapple leaf samples obtained from various parts of the Eastern Cape.
- Full Text:
- Authors: Davidson, Raymond John
- Date: 1958
- Subjects: Plants -- Nutrition , Boron , Turmeric
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4458 , http://hdl.handle.net/10962/d1011003
- Description: Methods for the determination of boron are reviewed. A thorough investigation of the curcumin method was undertaken. Factors such as spectral absorption, boron volatilisation and the effects of pH, the stability of reagents and colour solutions, the drying of reaction products and the interference from certain elements, were investigated. A study of the efficiency of extracting boron from plant material using an ashing procedure is presented. The modified curcumin method for the determination of boron in plant material is described. The statistical accuracy and precision of the modified method is presented. The modified method was used to determine the boron content in citrus and pineapple leaf samples obtained from various parts of the Eastern Cape.
- Full Text:
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