An investigation into the interaction effects of simultaneous physical and cognitive task execution on performance, perceptual and physical responses
- Authors: Ross, Natalie
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3562 , vital:20525
- Description: Many modern day work environments require some degree of dual tasking, particularly the simultaneous performance of cognitive jobs alongside physical activity. The nature of such tasks is often job dependent and may require cognitive functions such as perception, decision making, memory, or response selection/execution in conjunction to task specific physical requirements. Previous research has indicated a possible relationship between concurrent physical and cognitive demands and task performance, safety and efficiency. However, this research is limited and inconsistent. The current study aimed to identify the interaction, if any, between concurrent physical and mental demands, and determine the impact of sensory modality and stage of information processing on this interaction, with specific focus on the performance, perceptual and physical responses during different types of cognitive tasks performed concurrently with a lifting task. 20 (10 male, 10 female) Rhodes University students participated in this study. Each participant performed 9 test conditions - a physical lifting task, a visual and an auditory memory task, and a visual and an auditory decision making task in isolation, as well as the lifting task concurrently with each of these cognitive tasks. Performance was recorded via accuracy and work output of cognitive tasks and the number of lifts for each condition. Perceptual measures were obtained via the Borg RPE and Subjective Workload Assessment Technique. Spinal kinematics were measured using the Lumbar Motion Monitor, while muscle activity of the Erector Spinae, Rectus Abdominis, Rectus Femoris and Biceps Femoris muscles were recorded using the Biometrix Data Logger surface EMG equipment. Mean results were analysed using a dependent T-test to observe any general interaction, and a Two-way ANOVA for the impact of sensory modality and stage of processing. Individual responses were also considered to gain better understanding of both intra and inter-human variability under the various test conditions. Results showed a significant decrease in cognitive performance, increased perception of physical effort, time pressure, mental effort and psychological stress under simultaneous physical and mental demands, while no significant differences in physical responses were observed. Further observations included increased dual-task interference during visual and decision-making tasks when combined with physical demands compared to that of auditory and memory tasks respectively. Individual responses showed large variability between individuals indicating the presence of positive, negative and non-responders to concurrent physical and mental demands. Results therefore imply an individual specific interaction between concurrent physical and mental demands that may or may not be detrimental to worker productivity, job error, injury rates and worker well-being, and that the type of cognitive task performed may impact this interaction.
- Full Text:
- Authors: Ross, Natalie
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3562 , vital:20525
- Description: Many modern day work environments require some degree of dual tasking, particularly the simultaneous performance of cognitive jobs alongside physical activity. The nature of such tasks is often job dependent and may require cognitive functions such as perception, decision making, memory, or response selection/execution in conjunction to task specific physical requirements. Previous research has indicated a possible relationship between concurrent physical and cognitive demands and task performance, safety and efficiency. However, this research is limited and inconsistent. The current study aimed to identify the interaction, if any, between concurrent physical and mental demands, and determine the impact of sensory modality and stage of information processing on this interaction, with specific focus on the performance, perceptual and physical responses during different types of cognitive tasks performed concurrently with a lifting task. 20 (10 male, 10 female) Rhodes University students participated in this study. Each participant performed 9 test conditions - a physical lifting task, a visual and an auditory memory task, and a visual and an auditory decision making task in isolation, as well as the lifting task concurrently with each of these cognitive tasks. Performance was recorded via accuracy and work output of cognitive tasks and the number of lifts for each condition. Perceptual measures were obtained via the Borg RPE and Subjective Workload Assessment Technique. Spinal kinematics were measured using the Lumbar Motion Monitor, while muscle activity of the Erector Spinae, Rectus Abdominis, Rectus Femoris and Biceps Femoris muscles were recorded using the Biometrix Data Logger surface EMG equipment. Mean results were analysed using a dependent T-test to observe any general interaction, and a Two-way ANOVA for the impact of sensory modality and stage of processing. Individual responses were also considered to gain better understanding of both intra and inter-human variability under the various test conditions. Results showed a significant decrease in cognitive performance, increased perception of physical effort, time pressure, mental effort and psychological stress under simultaneous physical and mental demands, while no significant differences in physical responses were observed. Further observations included increased dual-task interference during visual and decision-making tasks when combined with physical demands compared to that of auditory and memory tasks respectively. Individual responses showed large variability between individuals indicating the presence of positive, negative and non-responders to concurrent physical and mental demands. Results therefore imply an individual specific interaction between concurrent physical and mental demands that may or may not be detrimental to worker productivity, job error, injury rates and worker well-being, and that the type of cognitive task performed may impact this interaction.
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Isolation and identification of Beta-Lactam Producing Microorganisms using PCR based methodologies
- Authors: Krallis, Myrsini
- Date: 1997
- Subjects: Polymerase chain reaction , Bacterial genetics , Fungi -- Genetics , Beta lactam antibiotics , Microbial enzymes
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4155 , http://hdl.handle.net/10962/d1018237
- Description: The polymerase chain reaction (PCR) was investigated as a potential tool in microbial screening for 13-lactam. producing organisms. Optimization of PCR conditions and the addition of acetamide to the PCR reaction allowed for the successful amplification of the isopenicillin N synthetase (lPNS) gene in S. clavuligerus, S. tanashiensis, S. griseus, S. olivaceus, S. lipmanii, and S. chartreusis. PCR was used to produce a radiolabelled probe from S. clavuligerus that was used to detect analogous genes in bacteria and fungi. Southern blot and dot blot analysis using the lPNS probe revealed the presence of IPNS-like sequences in seventeen organisms. Fourteen of these sequences belonged to known 13-lactam. producing organisms; one unidentified soil isolate; and two non-/3-lactam. producing organisms viz. S. venezuelae ATCC 10712 and S. hygroscopicus ATCC 21703. The lPNS gene was also detected in a 13-lactam producer (S. chartreusis) that had lost its ability to produce antibiotic. It would therefore have been overlooked in a conventional antibiotic screening program. The use of PCR, coupled with Southern hybridization and dot blot analysis, increased the sensitivity and specificity of the antibiotic screening procedures and allowed for the investigation of evolutionary relationships between the eukaryotes and the prokaryotes. A preliminary investigation into the potential use of RAPD PCR and protein fmgerprinting as tools for solving discrepancies in streptomycete identification was conducted. A variety of streptomycete species that were chosen as being representative of a number of numerical taxonomic classes were amplified using various RAPD primers. Streptomycetes appear to be genetically diverse organisms as was reflected by their RAPD and protein profiles. The application of PCR in an antibiotic screening program showed great potential as a specific and sensitive tool in the detection of /3-lactam producers and in the elimination of duplicate strains.
- Full Text:
- Authors: Krallis, Myrsini
- Date: 1997
- Subjects: Polymerase chain reaction , Bacterial genetics , Fungi -- Genetics , Beta lactam antibiotics , Microbial enzymes
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4155 , http://hdl.handle.net/10962/d1018237
- Description: The polymerase chain reaction (PCR) was investigated as a potential tool in microbial screening for 13-lactam. producing organisms. Optimization of PCR conditions and the addition of acetamide to the PCR reaction allowed for the successful amplification of the isopenicillin N synthetase (lPNS) gene in S. clavuligerus, S. tanashiensis, S. griseus, S. olivaceus, S. lipmanii, and S. chartreusis. PCR was used to produce a radiolabelled probe from S. clavuligerus that was used to detect analogous genes in bacteria and fungi. Southern blot and dot blot analysis using the lPNS probe revealed the presence of IPNS-like sequences in seventeen organisms. Fourteen of these sequences belonged to known 13-lactam. producing organisms; one unidentified soil isolate; and two non-/3-lactam. producing organisms viz. S. venezuelae ATCC 10712 and S. hygroscopicus ATCC 21703. The lPNS gene was also detected in a 13-lactam producer (S. chartreusis) that had lost its ability to produce antibiotic. It would therefore have been overlooked in a conventional antibiotic screening program. The use of PCR, coupled with Southern hybridization and dot blot analysis, increased the sensitivity and specificity of the antibiotic screening procedures and allowed for the investigation of evolutionary relationships between the eukaryotes and the prokaryotes. A preliminary investigation into the potential use of RAPD PCR and protein fmgerprinting as tools for solving discrepancies in streptomycete identification was conducted. A variety of streptomycete species that were chosen as being representative of a number of numerical taxonomic classes were amplified using various RAPD primers. Streptomycetes appear to be genetically diverse organisms as was reflected by their RAPD and protein profiles. The application of PCR in an antibiotic screening program showed great potential as a specific and sensitive tool in the detection of /3-lactam producers and in the elimination of duplicate strains.
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The taxonomy and life history of the blue stingray, Dasyatis marmorata capensis (Batoidea : Dasyatidae) from Southern Africa
- Cowley, Paul D (Paul Denfer), 1964-
- Authors: Cowley, Paul D (Paul Denfer), 1964-
- Date: 1990
- Subjects: Stingrays -- Africa, Southern Dasyatidae
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5183 , http://hdl.handle.net/10962/d1001959
- Description: The blue stingray, Dasyatis marmorata capensis is an important recreational shore angling species, especially amongst tournament anglers, in South Africa. The taxonomy and aspects of the biology of this species were investigated from specimens collected between March 1987 and May 1989, from various localities along the southern African coastline. Morphological comparisons were made between Dasyatis pastinaca, Dasyatis marmorata and the local variety. The results indicated that the local variety is different to D. pastinaca in colouration, disc length size, and vertebral and radial counts. The proposed new sub-species Dasyatis marmorata capensis is closest to Dasyatis marmorata of the north-eastern Atlantic coast, but differs in snout angle, disc length and snout to vent length. Analysis of catch per unit of effort data revealed that the blue stingray exhibits distinct seasonality. Adult and sub-adult fish undertake an annual onshore/offshore migration and invade the inshore region between spring and summer, while juveniles remain offshore and utilize this zone as a nursery area. Length-at-age and growth rates for both sexes were estimated from bands formed seasonally in the vertebral centra. The derived von Bertalanffy growth parameters indicated that females reach a greater asymptotic size (913.8 mm DW) and have a slower growth rate (K = -0.07) than males (531.8 mm DW, K = -0.17). Growth rates of juvenile captive specimens compared favourably with back calculated values, at approximately 65 mm/year, for the first year of growth. Dasyatis marmorata capensis is an aplacental viviparous elasmobranch with a well defined annual breeding cycle. Young are released , at a size of 170-200 mm DW, from October to December after a gestation period of approximately nine months. An average fecundity of 3.07 was calculated . Examination of gonadal development indicated that sexual maturity is first attained at a size of 408 and 500 mm DW in males and females, respectively, corresponding to ages 4.5 and 7 years. Size-related food habits were determined independently from three depth zones. The major prey items for each size class were directly related to the abundance of the infauna of the different depth zones. Polychaeta, amphipoda and stomatopoda were the major prey items in the offshore zone. In the nearshore zone, the dominant prey species were Balanoglossus capensis and Callianassa spp .. The high incidence of empty stomachs and the presence of prey items which occur in greatest abundance beyond the surf zone indicated that this zone is not utilized for the purposes of feeding.
- Full Text:
- Authors: Cowley, Paul D (Paul Denfer), 1964-
- Date: 1990
- Subjects: Stingrays -- Africa, Southern Dasyatidae
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5183 , http://hdl.handle.net/10962/d1001959
- Description: The blue stingray, Dasyatis marmorata capensis is an important recreational shore angling species, especially amongst tournament anglers, in South Africa. The taxonomy and aspects of the biology of this species were investigated from specimens collected between March 1987 and May 1989, from various localities along the southern African coastline. Morphological comparisons were made between Dasyatis pastinaca, Dasyatis marmorata and the local variety. The results indicated that the local variety is different to D. pastinaca in colouration, disc length size, and vertebral and radial counts. The proposed new sub-species Dasyatis marmorata capensis is closest to Dasyatis marmorata of the north-eastern Atlantic coast, but differs in snout angle, disc length and snout to vent length. Analysis of catch per unit of effort data revealed that the blue stingray exhibits distinct seasonality. Adult and sub-adult fish undertake an annual onshore/offshore migration and invade the inshore region between spring and summer, while juveniles remain offshore and utilize this zone as a nursery area. Length-at-age and growth rates for both sexes were estimated from bands formed seasonally in the vertebral centra. The derived von Bertalanffy growth parameters indicated that females reach a greater asymptotic size (913.8 mm DW) and have a slower growth rate (K = -0.07) than males (531.8 mm DW, K = -0.17). Growth rates of juvenile captive specimens compared favourably with back calculated values, at approximately 65 mm/year, for the first year of growth. Dasyatis marmorata capensis is an aplacental viviparous elasmobranch with a well defined annual breeding cycle. Young are released , at a size of 170-200 mm DW, from October to December after a gestation period of approximately nine months. An average fecundity of 3.07 was calculated . Examination of gonadal development indicated that sexual maturity is first attained at a size of 408 and 500 mm DW in males and females, respectively, corresponding to ages 4.5 and 7 years. Size-related food habits were determined independently from three depth zones. The major prey items for each size class were directly related to the abundance of the infauna of the different depth zones. Polychaeta, amphipoda and stomatopoda were the major prey items in the offshore zone. In the nearshore zone, the dominant prey species were Balanoglossus capensis and Callianassa spp .. The high incidence of empty stomachs and the presence of prey items which occur in greatest abundance beyond the surf zone indicated that this zone is not utilized for the purposes of feeding.
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Some aspects of the reproductive endocrinology of the catfish, Clarias Gariepinus (Burchell, 1822)
- Authors: Rennie, Susan Elizabeth
- Date: 1981
- Subjects: Catfishes Clarias gariepinus
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5339 , http://hdl.handle.net/10962/d1005924
- Description: A description is given of the location, anatomy and morphology of the endocrine glands likely to be involved in controlling the reproductive cycle of C. gariepinus. Methods of identifying the secretory tissues in the light and electron microscopes are outlined. A review of the published results on homologous tissues from other teleosts is included and directions for further research on the endocrinology of C. gariepinus are discussed.
- Full Text:
- Authors: Rennie, Susan Elizabeth
- Date: 1981
- Subjects: Catfishes Clarias gariepinus
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5339 , http://hdl.handle.net/10962/d1005924
- Description: A description is given of the location, anatomy and morphology of the endocrine glands likely to be involved in controlling the reproductive cycle of C. gariepinus. Methods of identifying the secretory tissues in the light and electron microscopes are outlined. A review of the published results on homologous tissues from other teleosts is included and directions for further research on the endocrinology of C. gariepinus are discussed.
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Some properties of magnetospheric electrons observed west of Sanae
- Authors: Greener, James G
- Date: 1973
- Subjects: Magnetosphere
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5523 , http://hdl.handle.net/10962/d1012091 , Magnetosphere
- Description: The southern hemisphere has always been the neglected twin in the field ot geophysical research. The reasons for this are certainly not in the realm of science but in the history of man and his development in the northern half of the globe. Geophysically, however, our southern half provides a wealth of interesting problems and anomalies. Roederer (1966) explains the use of this word 'anomaly' by showing what some of the oddities of the southern hemisphere are, and by contrasting them with the corresponding regions of the earth across the equator. The principal feature is of course the geomagnetic surface field strength minimum at a point in the South Atlantic ocean very near the coast of Brazil. Intro., p. 1.
- Full Text:
- Authors: Greener, James G
- Date: 1973
- Subjects: Magnetosphere
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5523 , http://hdl.handle.net/10962/d1012091 , Magnetosphere
- Description: The southern hemisphere has always been the neglected twin in the field ot geophysical research. The reasons for this are certainly not in the realm of science but in the history of man and his development in the northern half of the globe. Geophysically, however, our southern half provides a wealth of interesting problems and anomalies. Roederer (1966) explains the use of this word 'anomaly' by showing what some of the oddities of the southern hemisphere are, and by contrasting them with the corresponding regions of the earth across the equator. The principal feature is of course the geomagnetic surface field strength minimum at a point in the South Atlantic ocean very near the coast of Brazil. Intro., p. 1.
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