Photo-physicochemical studies and photodynamic therapy activity of indium and gallium phthalocyanines
- Authors: Tshangana, Charmaine Sesethu
- Date: 2015
- Subjects: Quantum dots , Nanoparticles , Photochemotherapy , Phthalocyanines
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4548 , http://hdl.handle.net/10962/d1017928
- Description: The potential toxicity of seven different types of quantum dots without shell (L-cysteine-CdTe, TGA-CdTe, MPA-CdTe, TGA-CdSe) and with the shell (GSH-CdSe@ZnS, GSH-CdTe@ZnS,) with different capping agents were evaluated. The growth inhibitory effects of the various quantum dots on human pancreatic BON cancerous cells were determined. The least cytotoxic of the various quantum dots synthesized and the one displaying the lowest growth inhibitory potential and no embryotoxicity was determined to be the GSH-CdSe@ZnS quantum dots. The GSH-CdSe@ZnS quantum dots were then conjugated to gallium, aluminium and indium octacarboxy phthalocyanine and the photophysical behaviour of the conjugates studied for potential use in photodynamic therapy and imaging applications. The sizes, morphology, thermal stability and confirmation of successful conjugation was determined using X-ray diffraction (XRD), transmission electron microscopy (TEM), thermogravimetric analysis (TGA) and Fourier transform infrared spectroscopy (FTIR), respectively. The study was extended by conjugating amino functionalized magnetic nanoparticles (Fe₃O₄) to indium octacarboxy phthalocyanine to study the photophysical behaviour of the conjugate as a potential bi-functional anti-cancer agent (hyperthermia and photodynamic therapy applications). A three-in-one multifunctional nanocomposite comprising of the quantum dots, magnetic nanoparticles and indium octacarboxy phthalocyanine was developed with the aim of developing a multifunctional composite that is able detect, monitor and treat cancer. All conjugates showed improved and enhanced photophysical behaviour. Finally, GSH-CdSe@ZnS conjugated to aluminium octacarboxy phthalocyanine was applied in human pancreatic carcinoid BON cells. The conjugates induced cell death dose-dependently.
- Full Text:
- Date Issued: 2015
Marine anti-malarial isonitriles : a synthetic and computational study
- Authors: Adendorff, Matthew Ralph
- Date: 2011 , 2010-05-17
- Subjects: Isocyanides , Isocyanates , Marine pharmacology , Antimalarials , Antimalarials -- Development , Drug development
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4398 , http://hdl.handle.net/10962/d1006674 , Isocyanides , Isocyanates , Marine pharmacology , Antimalarials , Antimalarials -- Development , Drug development
- Description: The development of Plasmodium falciparum malarial resistance to the current armoury of anti-malarial drugs requires the development of new treatments to help combat this disease. The marine environment is a well established source of potential pharmaceuticals. Of interest to us are isonitrile, isocyanate and isothiocyanate compounds isolated from marine sponges and molluscs which have exhibited nano-molar anti-plasmodial activities. Through quantitative structure-activity relation studies (QSAR), a literature precedent exists for a pseudoreceptor model from which a pharmacophore for the design of novel anti-malarial agents was proposed. The current theory suggests that these marine compounds exert their inhibitory action through interfering with the heme detoxification pathway in P. falciparum. We propose that the computational methods used to draw detailed conclusions about the mode of action of these marine compounds were inadequate. This thesis addresses this problem using contemporary computational methodologies and seeks to propose a more robust method for the rational design of new anti-malarial drug compounds that inhibit heme polymerization to hemozoin. In order to investigate the interactions of the marine compounds with their heme targets, a series of modern computational procedures were formulated, validated and then applied to theoretical systems. The validations of these algorithms, before their application to the marine compound-heme systems, were achieved through two case studies. The first was used to investigate the applicability of the statistical docking algorithm AutoDock to be used for the exploration of conformational space around the heme target. A theoretical P. falciparum 1-deoxy-D-xylulose-5-phosphate reductoisomerase (PfDXR) enzyme model, constructed by the Biochemistry Department at Rhodes University, provided the ideal model to validate the AutoDock program. The protein model was accordingly subjected to rigorous docking simulations with over 30 different ligand molecules using the AutoDock algorithm which allowed for the docking algorithm’s limitations to be ascertained and improved upon. This investigation facilitated the successful validation of the protein model, which can now be used for the rational design of new PfDXR-inhibiting anti-plasmodial compounds, as well as enabling us to propose an improvement of the docking algorithm for application to the heme systems. The second case study was used to investigate the applicability of an ab initio molecular dynamics algorithm for simulation of bond breaking/forming events between the marine compounds and their heme target. This validation involved the exploration of intermolecular interactions in a naturally occurring nonoligomeric zipper using the Car-Parrinello Molecular Dynamics (CPMD) method. This study allowed us to propose a model for the intermolecular forces responsible for zipper self-assembly and showcased the CPMD method’s abilities to simulate and predict bond forming/breaking events. Data from the computational analyses suggested that the interactions between marine isonitriles, isocyanates and isothiocyanates occur through bond-less electrostatic attractions rather than through formal intermolecular bonds as had been previously suggested. Accordingly, a simple bicyclic tertiary isonitrile (5.14) was synthesized using Kitano et al’s relatively underutilized isonitrile synthetic method for the conversion of tertiary alcohols to their corresponding isonitriles. This compound’s potential for heme detoxification inhibition was then explored in vitro via the pyridine-hemochrome assay. The assay data suggested that the synthesized isonitrile was capable of inhibiting heme polymerization in a similar fashion to the known inhibitor chloroquine. Attempts to synthesize tricyclic analogues of 5.14 were unsuccessful and highlighted the limitation of Kitano et al’s isonitrile synthetic methodology.
- Full Text:
- Date Issued: 2011
Demographical diversity influence on online shopping orientation and propensity to buy online
- Authors: Tapson, Megan Jacqueline
- Date: 2010
- Subjects: Teleshopping -- South Africa , Electronic commerce -- South Africa , Consumer behavior -- South Africa -- Cross-cultural studies , Ethnicity -- South Africa , Ethnic attitudes -- South Africa , Consumer behavior -- South Africa
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:1168 , http://hdl.handle.net/10962/d1002784 , Teleshopping -- South Africa , Electronic commerce -- South Africa , Consumer behavior -- South Africa -- Cross-cultural studies , Ethnicity -- South Africa , Ethnic attitudes -- South Africa , Consumer behavior -- South Africa
- Description: Online shopping is a growing phenomenon all over the world, and it has thus had an influence on the shopping process for many consumers (Brengman et al., 2005:79; Brown et al., 2003:1667; Kau et al., 2003:139). Consumers are now choosing to shop online due to changes in their lifestyles and demographic diversity (Kau et al., 2003:139). The purpose of this research was to examine the extent to which demographic diversity influenced online shopping orientation and propensity to buy online. The researcher first conducted an in-depth theoretical study of previous research into demographic diversity, online shopping orientation and propensity to buy online. Next, the researcher conducted an empirical survey questionnaire, in order to assess the opinions of students at the three selected Eastern Cape universities. Phinney's (1992:156) multi-group ethnic identity measure was administered in order to identify how respondents feel about their ethnicity and/or how they react to their own ethnic group. Kau et al.,'s (2003) online shopping orientation measure was used to help identify six relevant online shopping factors. The six online shopping factors where then used to describe six online shopping orientation types. The main findings of this research conducted at three Universities in the Eastern Cape can be summarised as follows: Ethnic Identity Profile: - Within this research it was found that respondents at all three universities in general had a strong ethnic identity to their specified ethnic group, according to the two ethnic identity factors namely; commitment and exploration. Online Shopping Orientation Profile: - Within this research it was found that the respondents at all three universities in general could be categorised in one of six online shopping orientation types according to six online shopping factors. The extent to which demographic diversity influences types of online shopping orientation: - From the research hypotheses it was concluded that a relationship exists between demographic diversity and types of online shopping orientations, whereby the gender, race and ethnicity of respondent is likely to influence the way consumers shop online, namely the consumers‟ online shopping orientation. The extent to which demographic diversity influences propensity to buy online: - From the research hypotheses it was concluded that a relationship exists between demographic diversity and propensity to buy online, whereby the gender of respondents is likely to influence whether consumers buy online in the future. - From the research hypotheses it was concluded that no relationship exists between demographic diversity and propensity to buy online, whereby the race and ethnicity of respondents is not likely to influence whether consumers buy online in the future. The extent to which types of online shopping orientation influence propensity to buy online: - The findings suggest that different online shopping orientations have different propensities to buy online in the future. Hence, marketers should be aware of the online shopping orientations more likely to buy online in the future and focus their marketing programs on them. They should also try new ways to attract the online shopping orientations that may not buy online, in the hopes of changing their opinions and perceptions of the Internet and online shopping. South Africa, in particular, consists of multi-racial and diverse ethnic origins, which has resulted in a demographically diverse South African nation. Therefore, marketers need to develop a better understanding of consumers‟ online shopping orientation (Donthu and Garcia, 1999:57; Jayawardhena and Foley, 2000:19; Kau et al., 2003:140). Understanding consumers' online shopping orientation will assist marketers in being able to market to specific consumers and meet the needs of consumers effectively (Kau et al., 2003:140). Since demographic diversity influences the online shopping orientations of consumers, major opportunities for marketers will be provided by accommodating for a demographically diverse South African nation.
- Full Text:
- Date Issued: 2010
Movement patterns of spotted grunter, Pomadasys commersonnii (Haemulidae), in a highly turbid South African estuary
- Authors: Childs, Amber-Robyn
- Date: 2006
- Subjects: Grunts (Fishes) -- South Africa -- Great Fish River , Pomadasys -- South Africa -- Great Fish River , Freshwater fishes -- South Africa -- Great Fish River , Fishes -- Behavior -- South Africa -- Great Fish River , Estuarine fishes -- South Africa -- Great Fish River , Estaurine ecology -- South Africa -- Great Fish River , Marine ecology -- South Africa -- Great Fish River
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5304 , http://hdl.handle.net/10962/d1005149 , Grunts (Fishes) -- South Africa -- Great Fish River , Pomadasys -- South Africa -- Great Fish River , Freshwater fishes -- South Africa -- Great Fish River , Fishes -- Behavior -- South Africa -- Great Fish River , Estuarine fishes -- South Africa -- Great Fish River , Estaurine ecology -- South Africa -- Great Fish River , Marine ecology -- South Africa -- Great Fish River
- Description: The principal objective of this thesis was to gain an understanding of the movement patterns of spotted grunter Pomadasys commersonnii, an estuarine-dependent fishery species, in the turbid, freshwater dominated Great Fish Estuary. Both manual and automated telemetry methods were used to monitor the movements of spotted grunter during two separate studies conducted in summer and spring 2003 and 2004. Acoustic transmitters were surgically implanted into twenty spotted grunter with lengths between 263 and 387 mm TL in the first study and twenty spotted grunter ranging between 362 and 698 mm TL in the second study. The specific objectives were to gain an understanding of (i) the time spent in the estuarine environment (ii) the space use and home range size, and (iii) the abiotic factors governing the movement patterns of spotted grunter in the estuary. The nursery function of estuarine environments was highlighted in this study as adolescent spotted grunter spent a significantly larger proportion of their time in the estuary than adult fish (p < 0.0001; R² = 0.62). The increased frequency of sea trips, with the onset of sexual maturity, provided testimony of the end of the estuarinedependent phase of their life-cycle. Although considered to be predominantly marine, the adult spotted grunter in the Great Fish Estuary utilised the estuary for considerable periods. Adults are thought to frequent estuaries to forage, seek shelter and to possibly rid themselves of parasites. During this study, the number of sea trips made by tagged fish ranged from 0 to 53, and the duration ranged from 6 hours to 28 days. The tidal phase and time of day had a significant effect (p < 0.05) on the sea trips undertaken by fish. Most tagged spotted grunter left the estuary during the night (84%) on the outgoing tide, and most returned in the evening (77%) during the incoming tide. Sea temperature (p < 0.0001; R² = 0.34), barometric pressure (p = 0.004; R² = 0.19) and wind (p = 0.01) had a significant effect on the number of spotted grunter recorded in the estuary. Spotted grunter were more prone to return to the estuary after high barometric pressure, when low sea temperatures (upwelling events) prevailed. There was a significant positive relationship between home range size and fish length (p = 0.004; R² = 0.20). Small spotted grunter (< 450 mm TL) appeared to be highly resident, with a small home range (mean size = 129 167 m²), that was generally confined to a single core area. Larger individuals (> 450 mm TL) occupied larger home ranges (mean size = 218 435 m²) with numerous core areas. The home ranges of small and large spotted grunter overlapped considerably yielding evidence of two high use areas, situated 1.2 km and 7 km from the mouth of the Great Fish Estuary. Tagged spotted grunter were located in a wide range of salinity, turbidity and temperature, but were found to avoid temperatures below 16 ºC. The daily change in environmental variables (salinity, temperature and turbidity) had a significant effect on the change in fish position in the estuary (p < 0.0001; R² = 0.38). The distribution of tagged spotted grunter, particularly the larger individuals, in the Great Fish Estuary was influenced by the tidal phase (p < 0.05); they moved upriver on the incoming tide and downriver on the outgoing tide. This study provides an understanding of the movement patterns of spotted grunter in the estuary and between the estuarine and marine environments. Consequently, it provides information that will assist in the design of a management plan to promote sustainability of this important fishery species. The techniques used and developed in this study also have direct application for further studies on other important estuarinedependent fishery species.
- Full Text:
- Date Issued: 2006
Design of a performance evaluation tool for multimedia databases with special reference to Oracle
- Authors: Stakemire, Tonia
- Date: 2004
- Subjects: Oracle (Computer file) , Application software -- Evaluation , Database management -- Evaluation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4705 , http://hdl.handle.net/10962/d1015975
- Description: Increased production and use of multimedia data has led to the development of a more advanced Database Management System (DBMS), like an Object Relational Database Management System (ORDBMS). These advanced databases are necessitated by the complexity in structure and the functionality required by multimedia data. Unfortunately, no suitable benchmarks exist with which to test the performance of databases when handling multimedia data. This thesis describes the design of a benchmark to measure the performance of basic functionality found in multimedia databases. The benchmark, called MORD (Multimedia Object Relational Databases), targets Oracle, a well known commercial Object Relational Database Management System (ORDBMS) that can handle multimedia data. Although MORD targets Oracle, it can easily be applied to other Multimedia Database Management System (MMDBMS) as a result of a design that stressed its portability, and simplicity. MORD consists of a database schema, test data, and code to simulate representative queries on multimedia databases. A number of experiments are described that validate MORD and ensure its correct design and that its objectives are met. A by-product of these experiments is an initial understanding of the performance of multimedia databases. The experiments show that with multimedia data the buffer cache should be at least large enough to hold the largest dataset, a bigger block size improves the performance, and turning off logging and caching for bulk loading improves the performance. MORD can be used to compare different ORDBMS or to assist in the configuration of a specific database.
- Full Text:
- Date Issued: 2004
A comparative analysis of Java and .NET mobile development environments for supporting mobile services
- Authors: Zhao, Xiaogeng
- Date: 2003 , 2013-05-23
- Subjects: Microsoft .NET , Java (Computer program language) , Mobile computing , Wireless communication systems
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4578 , http://hdl.handle.net/10962/d1003064 , Microsoft .NET , Java (Computer program language) , Mobile computing , Wireless communication systems
- Description: With the rapid development of wireless technologies, traditional mobile devices, such as pagers and cellular phones, have evolved from a purely communications and messaging-oriented medium to one that supports mobile data communication in general and acts as an application platform. As shown in a recent survey conducted by MDA, easy access to the present-day wireless Internet has resulted in mobile devices gaining more and more attention and popularity. The growth of and demand for mobile Web applications is expected to increase rapidly in the near future, as a range of software companies and mobile device manufacturers release increasingly accessible tools for creating mobile Web application and services. From a variety of possible development environments of this kind, the author has selected and examined two leading contenders, the J2ME and the Microsoft .NET mobile Web application development environments. This document reports the product life cycle of pilot mobile web applications, designed and implemented in each host environment in tum. A feature-by-feature investigation and comparison of the J2ME and .NET environments was carried out, covering the range of issues necessary for a complete mobile Web application development life cycle. The resulting analysis addresses features and efficiencies of the application development environment and the target deployment environment, the degree to which the resultant services are compatible on a variety of platforms, and the ease with which applications can be designed to be extensible. The thesis offers an objective evaluation of the J2ME and the .NET mobile development environments, which highlights their strengths and weaknesses, and suggests guidelines for designing, creating, and deploying high quality mobile Web applications. The research uncovers no clear winner across all categories assessed. J2ME currently favours situations in which bandwidth is limited and client side processing power is relatively sufficient, it exerts the processing power of mobile devices over distributed network environments. .NET requires a less constrained network throughput, but performs adequately on clients with more limited processing power, supports a more diverse target platform range, and offers a more efficient, in terms of development time, development environment. Both technologies are likely to receive significant user support for some time. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2003
An investigation into the possible neuroprotective properties of phenytoin
- Authors: Naga, Nishal
- Date: 2002
- Subjects: Phenytoin -- Therapeutic use , Phenytoin -- Physiological effect , Nervous system -- Degeneration -- Prevention
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3780 , http://hdl.handle.net/10962/d1003258 , Phenytoin -- Therapeutic use , Phenytoin -- Physiological effect , Nervous system -- Degeneration -- Prevention
- Description: Cerebral ischaemia, traumatic injury to the brain, inflammatory neurological disorders and HIV infections are amongst the most prevalent causes of neurodegeneration. Neuroprotective strategies are usually to limit the progressive secondary injury that generally occurs, thus limiting overall tissue damage. Neuroprotective strategies are usually to limit the progressive secondary injury that generally occurs, thus limiting overall tissue damage. Sodium channel blockers have been often used for this matter as they prevent the cascade of events culminating in free radical generation and eventually neuronal apoptosis. Newer compounds, such as antiperoxidants and free radical scavengers, show encouraging experimental results, but their clinical use is still very limited. Phenytoin being a popular drug in the treatment of epilepsy has also been used as a neuroprotectant during certain neurological emergencies and in pharmacological prophylaxis of post-traumatic epilepsy. Furthermore this agent functions by prolonging inactivation of voltage gated sodium channels. In these sets of experiment the neuroprotective properties of phenytoin were examined. The histological study revealed that phenytoin confers protection to the CA1 and CA3 regions of the hippocampus under the insult of QUIN. Cells maintain their characteristic shape and minimal tissue necrosis occurs in the presence of this agent. The in vitro effect of this antiepileptic drug on free radicals generation shows that phenytoin does not reduce or prevent the formation of these reactive species. Lipid peroxidation was induced using QUIN and iron (II), two known neurotoxins. The study reveals that only lipid peroxidation induced using iron (II) is reduced by phenytoin. These experiments were carried out in whole rat brain homogenate. These studies show that phenytoin possesses poor free radical scavenging properties. However, the dose-related reduction of iron-induced lipid peroxidation allows for speculation that phenytoin interacts with iron in order to reduce neuronal damage. Metal binding studies were performed using UV, IR and electrochemical analysis to examine the interaction of phenytoin with iron (II) and iron (III). Phenytoin, when added to iron (II) in solution, first oxidises the latter to iron (III) and maintains it in that form. A shift in the peak was observed in the UV spectrum when iron was added to phenytoin. Moreover, electrochemical studies indicate that the interaction between the metal and the ligand is very weak. The IR analysis it shows that phenytoin may be coordinating with iron through the Nitrogen atom on the phenytoin molecule. These studies show that phenytoin maintains iron in its oxidised form, which is a good property to possess as a neuroprotectants. Pineal organ culture showed that phenytoin does not increase melatonin production but slightly and non-significantly reduces the levels of this pineal hormone. However there is a significant rise in precursor NAS levels. As melatonin is known to possess antioxidant and free radical scavenging properties, this could mean that this drug can cause the CNS to become more susceptible to attacks by reactive oxygen species.
- Full Text:
- Date Issued: 2002
The alluvial diamond deposits of the lower Vaal river between Barkly West and the Vaal-Harts confluence in the Northern Cape province, South Africa
- Authors: Matheys, Fabrice Gilbert
- Date: 1991
- Subjects: Diamond deposits -- South Africa , Geology, Economic
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4933 , http://hdl.handle.net/10962/d1005545 , Diamond deposits -- South Africa , Geology, Economic
- Description: The alluvial diamond deposits along the Vaal River, between Barkly West and the Vaal-Harts confluence, have been worked for more than one century by thousands of private diggers. The diamonds are recovered from two sedimentary units of Cenozoic age, the Older Gravels and the Younger Gravels. These rest on a basement of Ventersdorp Supergroup andesites and Karoo Sequence sediments, which have been intruded by Cretaceous kimberlites. The gravels are, in turn, overlain by the Riverton Formation and the Hutton Sand. On a large scale, tectonic setting, geomorphology and palaeoclimate have played a major role in the formation of diamondiferous placers in the area under investigation. A study of the sedimentology of the Younger Gravels was carried out with the aim of acquiring an understanding of the processes responsible for the economic concentration of high quality diamonds. An investigation of facies assemblages, clast composition, clast size, palaeocurrent directions external geometry, particle morphology and led to the conclusion that the Younger Gravels were deposited in a proximal braided stream environment during high discharge. A small-scale experiment was carried out to test the efficiency of different sedimentological trap sites in concentrating kimberlite indicator minerals. The results show that the concentration of indicator minerals is dependent on the size fraction chosen, bed roughness and gravel calibre. The examination of surface features on pyrope, picroilmenite and chrome diopside from kimberlite led to the conclusion that most of these minerals are locally derived. Diamond grade variations within the Younger Gravels are influenced by a combination of factors, including bed roughness, channel width and sorting process from the source. Alluvial diamond exploration programmes must take into account the tectonic setting, the palaeoclimate, the level of erosion, the stability of the drainage system in the area as well as the presence of local trap sites in the river profile. It is concluded that the diamonds are the product of a long and complex process of erosion, reworking and concentration and are derived from Cretaceous kimberlites in the area.
- Full Text:
- Date Issued: 1991
An investigation of the polarization of solar radio noise
- Authors: Verschuur, Gerrit L.,1937-
- Date: 1961
- Subjects: Solar radio emission , Polarimetry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5547 , http://hdl.handle.net/10962/d1013441
- Description: CHAPTER I A description of the sun and the type of radio radiation it emits is given. The relation that exists between this and other events occurring on the sun's surface is studied. CHAPTER II The literature dealing with the origin of solar radio noise is reviewed. CHAPTER III The method of specifying polarized radiation and the effect of a magneto- ionic medium on such radiation is discussed. The possible origin of the polarization of solar radio noise is examined and the literature relating to this and to the observations of polarization of solar noise is reviewed. A short outline of the methods used in measuring polarization is given. CHAPTER IV A detailed outline of the construction of a polarimeter is given together with full circuit diagrams and illustrative photographs. CHAPTER V A brief discussion of the operation of the polarimeter, the results obtained and suggestions for its future operation is given.
- Full Text:
- Date Issued: 1961