Evaluation of a plant-herbivore system in determining potential efficacy of a candidate biological control agent, cornops aquaticum for water hyacinth, eichhornia crassipes
- Authors: Bownes, Angela
- Date: 2009
- Subjects: Water hyacinth -- Control -- South Africa , Eichhornia crassipedes , Pontederiaceae , Grasshoppers , Biological pest control agents -- South Africa , Weeds -- Biological control -- South Africa , Invasive plants -- Biological control -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5687 , http://hdl.handle.net/10962/d1005373
- Description: Water hyacinth, Eichhornia crassipes Mart. Solms-Laubach (Pontederiaceae), a freefloating aquatic macrophyte of Neotropical origin, was introduced into South Africa as an ornamental aquarium plant in the early 1900’s. By the 1970’s it had reached pest proportions in dams and rivers around the country. Due to the sustainability, cost efficiency and low environmental risk associated with biological control, this has been a widely used method in an attempt to reduce infestations to below the threshold where they cause economic and ecological damage. To date, five arthropod and one pathogen biocontrol agents have been introduced for the control of water hyacinth but their impact has been variable. It is believed that their efficacy is hampered by the presence of highly eutrophic systems in South Africa in which plant growth is prolific and the negative effects of herbivory are therefore mitigated. It is for these reasons that new, potentially more damaging biocontrol agents are being considered for release. The water hyacinth grasshopper, Cornops aquaticum Brüner (Orthoptera: Acrididae), which is native to South America and Mexico, was brought into quarantine in Pretoria, South Africa in 1995. Although the grasshopper was identified as one of the most damaging insects associated with water hyacinth in its native range, it has not been considered as a biocontrol agent for water hyacinth anywhere else in the world. After extensive host-range testing which revealed it to be safe for release, a release permit for this candidate agent was issued in 2007. However, host specificity testing is no longer considered to be the only important component of pre-release screening of candidate biocontrol agents. Investigating biological and ecological aspects of the plant-herbivore system that will assist in determination of potential establishment, efficacy and the ability to build up good populations in the recipient environment are some of the important factors. This thesis is a pre-release evaluation of C. aquaticum to determine whether it is sufficiently damaging to water hyacinth to warrant its release. It investigated interactions between the grasshopper and water hyacinth under a range of nutrient conditions found in South African water bodies as well as the impact of the grasshopper on the competitive performance of water hyacinth. Both plant growth rates and the response of water hyacinth to herbivory by the grasshopper were influenced by nutrient availability to the plants. The ability of water hyacinth to compensate for loss of tissue through herbivory was greater under eutrophic nutrient conditions. However, a negative linear relationship was found between grasshopper biomass and water hyacinth performance parameters such as biomass accumulation and leaf production, even under eutrophic conditions. Water hyacinth’s compensatory ability in terms of its potential to mitigate to detrimental effects of insect feeding was dependent on the amount of damage caused by herbivory by the grasshopper. Plant biomass and the competitive ability of water hyacinth in relation to another freefloating aquatic weed species were reduced by C. aquaticum under eutrophic nutrient conditions, in a short space of time. It was also found that grasshopper feeding and characteristics related to their population dynamics such as fecundity and survival were significantly influenced by water nutrient availability and that environmental nutrient availability will influence the control potential of this species should it be released in South Africa. Cornops aquaticum shows promise as a biocontrol agent for water hyacinth but additional factors that were not investigated in this study such as compatibility with the South African climate and the current water hyacinth biocontrol agents need to be combined with these data to make a decision on its release. Possible management options for this species if it is to be introduced into South Africa are discussed.
- Full Text:
- Authors: Bownes, Angela
- Date: 2009
- Subjects: Water hyacinth -- Control -- South Africa , Eichhornia crassipedes , Pontederiaceae , Grasshoppers , Biological pest control agents -- South Africa , Weeds -- Biological control -- South Africa , Invasive plants -- Biological control -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5687 , http://hdl.handle.net/10962/d1005373
- Description: Water hyacinth, Eichhornia crassipes Mart. Solms-Laubach (Pontederiaceae), a freefloating aquatic macrophyte of Neotropical origin, was introduced into South Africa as an ornamental aquarium plant in the early 1900’s. By the 1970’s it had reached pest proportions in dams and rivers around the country. Due to the sustainability, cost efficiency and low environmental risk associated with biological control, this has been a widely used method in an attempt to reduce infestations to below the threshold where they cause economic and ecological damage. To date, five arthropod and one pathogen biocontrol agents have been introduced for the control of water hyacinth but their impact has been variable. It is believed that their efficacy is hampered by the presence of highly eutrophic systems in South Africa in which plant growth is prolific and the negative effects of herbivory are therefore mitigated. It is for these reasons that new, potentially more damaging biocontrol agents are being considered for release. The water hyacinth grasshopper, Cornops aquaticum Brüner (Orthoptera: Acrididae), which is native to South America and Mexico, was brought into quarantine in Pretoria, South Africa in 1995. Although the grasshopper was identified as one of the most damaging insects associated with water hyacinth in its native range, it has not been considered as a biocontrol agent for water hyacinth anywhere else in the world. After extensive host-range testing which revealed it to be safe for release, a release permit for this candidate agent was issued in 2007. However, host specificity testing is no longer considered to be the only important component of pre-release screening of candidate biocontrol agents. Investigating biological and ecological aspects of the plant-herbivore system that will assist in determination of potential establishment, efficacy and the ability to build up good populations in the recipient environment are some of the important factors. This thesis is a pre-release evaluation of C. aquaticum to determine whether it is sufficiently damaging to water hyacinth to warrant its release. It investigated interactions between the grasshopper and water hyacinth under a range of nutrient conditions found in South African water bodies as well as the impact of the grasshopper on the competitive performance of water hyacinth. Both plant growth rates and the response of water hyacinth to herbivory by the grasshopper were influenced by nutrient availability to the plants. The ability of water hyacinth to compensate for loss of tissue through herbivory was greater under eutrophic nutrient conditions. However, a negative linear relationship was found between grasshopper biomass and water hyacinth performance parameters such as biomass accumulation and leaf production, even under eutrophic conditions. Water hyacinth’s compensatory ability in terms of its potential to mitigate to detrimental effects of insect feeding was dependent on the amount of damage caused by herbivory by the grasshopper. Plant biomass and the competitive ability of water hyacinth in relation to another freefloating aquatic weed species were reduced by C. aquaticum under eutrophic nutrient conditions, in a short space of time. It was also found that grasshopper feeding and characteristics related to their population dynamics such as fecundity and survival were significantly influenced by water nutrient availability and that environmental nutrient availability will influence the control potential of this species should it be released in South Africa. Cornops aquaticum shows promise as a biocontrol agent for water hyacinth but additional factors that were not investigated in this study such as compatibility with the South African climate and the current water hyacinth biocontrol agents need to be combined with these data to make a decision on its release. Possible management options for this species if it is to be introduced into South Africa are discussed.
- Full Text:
Effects of coastal topography on physiology, behaviour and genetics of indigenous (Perna perna) and invasive (Mytilus galloprovincialis) mussels
- Authors: Nicastro, Katy R
- Date: 2008
- Subjects: Mussels -- Ecology -- South Africa , Perna -- Physiology -- South Africa , Perna -- Behavior -- South Africa , Mussels -- Behavior -- Environmental factors -- South Africa , Mussels -- Habitat -- South Africa , Mytilus galloprovincialis , Mytilus galloprovincialis -- Physiology -- South Africa , Mytilus galloprovincialis -- Behavior -- South Africa , Mytilus galloprovincialis -- Genetics -- South Africa , Coastal ecology -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5833 , http://hdl.handle.net/10962/d1008262
- Description: Organisms inhabit environments that have many dimensions, each of which can vary temporally and spatially. The spatial-temporal variations of environmental stressors and disturbances may have major but different effects on indigenous and invasive species, favouring either of them at different times and places. The invasive mussel Mytilus galloprovincialis invaded the South African coast 30 years ago and, on the south coast of South Africa, it now competes and co-exists with the indigenous Perna perna in the lower eulittoral zone (referred to here as the mussel zone) The invasive and indigenous species dominate the upper and the lower mussel zones respectively, while the two co-exist in the mid-zone. My results show that intertidal mussels experience, and respond to, spatial and temporal fluctuations of several biotic and abiotic stressors. The invasive and the indigenous species adopt different strategies when reacting to environmental factors and their physiological and behavioural responses vary in time and in different habitats as different pressures become of overriding importance. Attachment strength of both species decreased in summer and increased in winter, and was higher on the open coast than in bays for both species, showing a strong positive correlation with wave force in time and space. P. perna had significantly higher attachment strength than M. galloprovincialis but, contrary to previous studies, the difference in gonad index between the two species varied according to the habitat. In bay habitats, M. galloprovincialis had a higher maximum reproductive effort than P. perna, however, on the open coast, there was no significant difference between the two species, suggesting that for the invasive species wave action is a limiting factor not only in terms of the attachment strength but also of energy availability for reproductive tissue development. Major spawning events occurred during periods of low wave action while minor spawning coincided with periods of intense hydrodynamic stress. On the open coast, gonad index was negatively correlated with attachment strength for both species while, in bays, there was no correlation between these two factors for either. The two species also showed different behaviour. In the field, M. galloprovincialis moved significantly more than P. perna over a period of six months. The higher mobility of the invasive species was also confirmed in the laboratory where, in general, M. galloprovincialis formed clumps more readily than P. perna. Taken collectively, these results suggest that channelling more energy into attachment strength limits reproductive tissue development and that, while the indigenous species invests more in byssal production, the invasive species adopts a more dynamic strategy looking for aggregation or a safer arrangement. Higher endolithic infestation and a greater expression of heat shock proteins (Hsps) in mussel populations on the open coast than in bays indicate that this habitat is a more stressful environment not only in terms of wave action. Endolith damaged mussels had significantly lower attachment strengths and condition indices than clean mussels, probably due to the need to channel energy into shell repair. The constant shell repair and expression of Hsps typical of open coast populations are energetically demanding processes. These observations suggest that on the open coast, mussels are subjected to more severe energetic constraints than in bay habitats. Wave and sand stress fluctuated seasonally with the former having a greater effect on mussel mortality on the open coast and the latter a higher impact on bay populations. Overall, mussel mortality rates were higher on the open coast than in bays. My results show that populations on the open coast had fewer private haplotypes and less genetic endemism than those inside bays. Gene flow analysis showed the relatively stable bay habitats act as source populations with greater genetic migration rates out of bays than into them. These differences in genetic structure on scales of las of kilometers show that coastal configuration strongly affects selection, larval dispersal and haplotype diversity. Environmental gradients that are key factors in species distribution over large geographical scales can also be responsible for micro-scale distributions. My results show that M. galloprovincialis colonizes the upper mussel zone where temperature is high, but is less tolerant to this stressor and has to maintain a high expression of Hsps. This suggests that temperature is probably a limiting factor in its invasion towards the sub-tropical east coast. There are inter- and intra-specific differences in responses to the environment which highlight the efforts of M. galloprovincialis and P. perna to optimize resource utilization for survival and reproduction. Determining these differences is crucial to understanding patterns of co-existence between competing indigenous and invasive species.
- Full Text:
- Authors: Nicastro, Katy R
- Date: 2008
- Subjects: Mussels -- Ecology -- South Africa , Perna -- Physiology -- South Africa , Perna -- Behavior -- South Africa , Mussels -- Behavior -- Environmental factors -- South Africa , Mussels -- Habitat -- South Africa , Mytilus galloprovincialis , Mytilus galloprovincialis -- Physiology -- South Africa , Mytilus galloprovincialis -- Behavior -- South Africa , Mytilus galloprovincialis -- Genetics -- South Africa , Coastal ecology -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5833 , http://hdl.handle.net/10962/d1008262
- Description: Organisms inhabit environments that have many dimensions, each of which can vary temporally and spatially. The spatial-temporal variations of environmental stressors and disturbances may have major but different effects on indigenous and invasive species, favouring either of them at different times and places. The invasive mussel Mytilus galloprovincialis invaded the South African coast 30 years ago and, on the south coast of South Africa, it now competes and co-exists with the indigenous Perna perna in the lower eulittoral zone (referred to here as the mussel zone) The invasive and indigenous species dominate the upper and the lower mussel zones respectively, while the two co-exist in the mid-zone. My results show that intertidal mussels experience, and respond to, spatial and temporal fluctuations of several biotic and abiotic stressors. The invasive and the indigenous species adopt different strategies when reacting to environmental factors and their physiological and behavioural responses vary in time and in different habitats as different pressures become of overriding importance. Attachment strength of both species decreased in summer and increased in winter, and was higher on the open coast than in bays for both species, showing a strong positive correlation with wave force in time and space. P. perna had significantly higher attachment strength than M. galloprovincialis but, contrary to previous studies, the difference in gonad index between the two species varied according to the habitat. In bay habitats, M. galloprovincialis had a higher maximum reproductive effort than P. perna, however, on the open coast, there was no significant difference between the two species, suggesting that for the invasive species wave action is a limiting factor not only in terms of the attachment strength but also of energy availability for reproductive tissue development. Major spawning events occurred during periods of low wave action while minor spawning coincided with periods of intense hydrodynamic stress. On the open coast, gonad index was negatively correlated with attachment strength for both species while, in bays, there was no correlation between these two factors for either. The two species also showed different behaviour. In the field, M. galloprovincialis moved significantly more than P. perna over a period of six months. The higher mobility of the invasive species was also confirmed in the laboratory where, in general, M. galloprovincialis formed clumps more readily than P. perna. Taken collectively, these results suggest that channelling more energy into attachment strength limits reproductive tissue development and that, while the indigenous species invests more in byssal production, the invasive species adopts a more dynamic strategy looking for aggregation or a safer arrangement. Higher endolithic infestation and a greater expression of heat shock proteins (Hsps) in mussel populations on the open coast than in bays indicate that this habitat is a more stressful environment not only in terms of wave action. Endolith damaged mussels had significantly lower attachment strengths and condition indices than clean mussels, probably due to the need to channel energy into shell repair. The constant shell repair and expression of Hsps typical of open coast populations are energetically demanding processes. These observations suggest that on the open coast, mussels are subjected to more severe energetic constraints than in bay habitats. Wave and sand stress fluctuated seasonally with the former having a greater effect on mussel mortality on the open coast and the latter a higher impact on bay populations. Overall, mussel mortality rates were higher on the open coast than in bays. My results show that populations on the open coast had fewer private haplotypes and less genetic endemism than those inside bays. Gene flow analysis showed the relatively stable bay habitats act as source populations with greater genetic migration rates out of bays than into them. These differences in genetic structure on scales of las of kilometers show that coastal configuration strongly affects selection, larval dispersal and haplotype diversity. Environmental gradients that are key factors in species distribution over large geographical scales can also be responsible for micro-scale distributions. My results show that M. galloprovincialis colonizes the upper mussel zone where temperature is high, but is less tolerant to this stressor and has to maintain a high expression of Hsps. This suggests that temperature is probably a limiting factor in its invasion towards the sub-tropical east coast. There are inter- and intra-specific differences in responses to the environment which highlight the efforts of M. galloprovincialis and P. perna to optimize resource utilization for survival and reproduction. Determining these differences is crucial to understanding patterns of co-existence between competing indigenous and invasive species.
- Full Text:
The effect of elevated CO₂ on Phaseolus vulgaris L. cv Contender
- Authors: Mjwara, Jabulani Michael
- Date: 1997
- Subjects: Plants -- Effect of carbon dioxide on Kidney bean
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4215 , http://hdl.handle.net/10962/d1003784
- Description: The response of Phaseolus vulgaris L. cv. Contender grown in controlled environmental conditions, at either ambient or elevated (360 and 700 μmol mol ̄¹, respectively) CO₂ concentrations ([CO₂]), was monitored from 10 days after germination (DAG) until the onset of senescence. Elevated CO₂ had a pronounced effect on total plant height (TPH), leaf area (LA), dry weight (DW) accumulation and specific leaf area (SLA). All of these were significantly increased by elevated [CO₂] with the exception of SLA, which was significantly reduced. Except for higher initial relative growth rates (RGR) in CO₂-enriched plants, RGR did not differ significantly between the two CO₂ treatments throughout the remainder of growth period. While growth parameters clearly differed between CO₂ treatments, the effects of CO₂ on many physiological processes including net assimilation rate (NAR), Rubisco activity, and some foliar nutrient concentrations were largely transient. For example, CO₂ enrichment significantly increased NAR, but from 20 DAG onward, NAR declined to levels measured on plants grown under ambient CO₂. Similarly, the decline in both foliar N concentration and Rubisco activity in CO₂-enriched plants after 20 DAG was significantly greater than the decline observed for ambient CO₂ plants. Soluble leaf protein and total chlorophylls (a+b) were also significantly reduced in plants grown under elevated CO₂. Chlorophyll (a/b) ratios increased with time underelevated CO₂, indicating that the rate of decline of chlorophyll b was higher than that of chorophyll α. No significant changes in total carotenoid (x+c) levels were observed in either CO₂ treatment. Under enhanced CO₂, the foliar concentrations of K and Mn were increased significantly, while P, Ca, Fe and Zn were reduced significantly. However, changes in Mg and Cu concentrations were not significant. High CO₂-grown plants also exhibited pronounced leaf discoloration or chlorosis, coupled with a significant reduction in leaf longevity. The levels of non-structural carbohydrates (sucrose, glucose, fructose and starch) and nitrogenous compounds (nitrogen, total soluble proteins and free amino acids) were determined for leaves and developing seeds of P. vulgaris. Leaf tissue of elevated CO₂-grown plants accumulated significantly higher levels of both soluble sugars and starch. Leaf ultrastructure revealed considerable erilargement of starch grain sizes with surface areas more than five times larger compared to those of control plants. No apparent differences in structure and membrane integrity of chloroplasts in both CO₂ treatments were noted. Although ambient CO₂-grown plants had comparatively low levels of non-structural carbohydrates (NSC), they accumulated significantly higher levels of nitrogenous compounds. The levels of NSC were consistently higher in seeds of plants grown under elevated CO₂. In comparison to plants grown at elevated [CO₂], pods and seeds of ambient CO₂-grown plants had significantly larger pools of free amino compounds and N. Stomatal conductance (gs) declined significantly, as expected for plants grown under elevated CO₂. This was accompanied by a decline in transpiration rates (E). Reduced gs and E led to high AlE ratio, which meant improved water use efficiency (WUE) values for CO₂-enriched bean plants. Leaf carbon isotope discrimination (∆) against the heavier isotope of carbon (¹³C), has been used to select for high WUE in C₃ plants. In plants grown at elevated CO₂ concentration, ,1 was significantly reduced. Although ∆ was negatively correlated with WUE in both CO₂ treatments, the correlation was steeper and highly negative for CO₂-enriched plants. These results indicate underlying differences in gas-exchange physiology, including stomatal responses between ambient and elevated CO₂-grown plants. Photosynthetic acclimation was investigated using the response of assimilation to internal carbon dioxide concentration (A/C₁ curves). At early stages of growth, the initial slope of the A/C₁ response curve did not differ with CO₂ treatment. In contrast, CO₂-saturated photosynthetic rate (Amax) was significantly higher in plants grown under elevated versus ambient CO₂ at 15 DAG. However, at subsequent stages of growth both the initial slope and Amax declined in bean plants grown in elevated CO₂. Apparent carboxylation efficiency (ACE, estimated from the initial slope of A/C₁ response) values followed a similar trend and were significantly reduced in CO₂-enriched plants. These results indicate that acclimation or negative adjustment of photosynthesis may have been caused by a combination of both stomatal and biochemical limitations. Bean plants grown under conditions of elevated atmospheric CO₂ flowered 3 to 4 days earlier, and produced significantly more flowers and pods than plants grown at ambient conditions. Plants grown at elevated CO₂ aborted 22 and 20% more flowers and pods, respectively, than plants grown at ambient CO₂. Elevated CO₂ also significantly increased the number of tillers or lateral branches produced by plants, which contributed to a significant increase in pod number and seed yield in these plants. Although plants grown at elevated CO₂ produced on average 8 seeds per pod, while plants grown under ambient CO2 conditions produced 5 seeds per pod, the greater number of seeds was offset by lower seed weights in plants grown under _ elevated CO₂. Thus, despite high seed yield in beans grown under elevated CO₂, the harvest index (HI) did not change significantly between CO₂ treatments.
- Full Text:
- Authors: Mjwara, Jabulani Michael
- Date: 1997
- Subjects: Plants -- Effect of carbon dioxide on Kidney bean
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4215 , http://hdl.handle.net/10962/d1003784
- Description: The response of Phaseolus vulgaris L. cv. Contender grown in controlled environmental conditions, at either ambient or elevated (360 and 700 μmol mol ̄¹, respectively) CO₂ concentrations ([CO₂]), was monitored from 10 days after germination (DAG) until the onset of senescence. Elevated CO₂ had a pronounced effect on total plant height (TPH), leaf area (LA), dry weight (DW) accumulation and specific leaf area (SLA). All of these were significantly increased by elevated [CO₂] with the exception of SLA, which was significantly reduced. Except for higher initial relative growth rates (RGR) in CO₂-enriched plants, RGR did not differ significantly between the two CO₂ treatments throughout the remainder of growth period. While growth parameters clearly differed between CO₂ treatments, the effects of CO₂ on many physiological processes including net assimilation rate (NAR), Rubisco activity, and some foliar nutrient concentrations were largely transient. For example, CO₂ enrichment significantly increased NAR, but from 20 DAG onward, NAR declined to levels measured on plants grown under ambient CO₂. Similarly, the decline in both foliar N concentration and Rubisco activity in CO₂-enriched plants after 20 DAG was significantly greater than the decline observed for ambient CO₂ plants. Soluble leaf protein and total chlorophylls (a+b) were also significantly reduced in plants grown under elevated CO₂. Chlorophyll (a/b) ratios increased with time underelevated CO₂, indicating that the rate of decline of chlorophyll b was higher than that of chorophyll α. No significant changes in total carotenoid (x+c) levels were observed in either CO₂ treatment. Under enhanced CO₂, the foliar concentrations of K and Mn were increased significantly, while P, Ca, Fe and Zn were reduced significantly. However, changes in Mg and Cu concentrations were not significant. High CO₂-grown plants also exhibited pronounced leaf discoloration or chlorosis, coupled with a significant reduction in leaf longevity. The levels of non-structural carbohydrates (sucrose, glucose, fructose and starch) and nitrogenous compounds (nitrogen, total soluble proteins and free amino acids) were determined for leaves and developing seeds of P. vulgaris. Leaf tissue of elevated CO₂-grown plants accumulated significantly higher levels of both soluble sugars and starch. Leaf ultrastructure revealed considerable erilargement of starch grain sizes with surface areas more than five times larger compared to those of control plants. No apparent differences in structure and membrane integrity of chloroplasts in both CO₂ treatments were noted. Although ambient CO₂-grown plants had comparatively low levels of non-structural carbohydrates (NSC), they accumulated significantly higher levels of nitrogenous compounds. The levels of NSC were consistently higher in seeds of plants grown under elevated CO₂. In comparison to plants grown at elevated [CO₂], pods and seeds of ambient CO₂-grown plants had significantly larger pools of free amino compounds and N. Stomatal conductance (gs) declined significantly, as expected for plants grown under elevated CO₂. This was accompanied by a decline in transpiration rates (E). Reduced gs and E led to high AlE ratio, which meant improved water use efficiency (WUE) values for CO₂-enriched bean plants. Leaf carbon isotope discrimination (∆) against the heavier isotope of carbon (¹³C), has been used to select for high WUE in C₃ plants. In plants grown at elevated CO₂ concentration, ,1 was significantly reduced. Although ∆ was negatively correlated with WUE in both CO₂ treatments, the correlation was steeper and highly negative for CO₂-enriched plants. These results indicate underlying differences in gas-exchange physiology, including stomatal responses between ambient and elevated CO₂-grown plants. Photosynthetic acclimation was investigated using the response of assimilation to internal carbon dioxide concentration (A/C₁ curves). At early stages of growth, the initial slope of the A/C₁ response curve did not differ with CO₂ treatment. In contrast, CO₂-saturated photosynthetic rate (Amax) was significantly higher in plants grown under elevated versus ambient CO₂ at 15 DAG. However, at subsequent stages of growth both the initial slope and Amax declined in bean plants grown in elevated CO₂. Apparent carboxylation efficiency (ACE, estimated from the initial slope of A/C₁ response) values followed a similar trend and were significantly reduced in CO₂-enriched plants. These results indicate that acclimation or negative adjustment of photosynthesis may have been caused by a combination of both stomatal and biochemical limitations. Bean plants grown under conditions of elevated atmospheric CO₂ flowered 3 to 4 days earlier, and produced significantly more flowers and pods than plants grown at ambient conditions. Plants grown at elevated CO₂ aborted 22 and 20% more flowers and pods, respectively, than plants grown at ambient CO₂. Elevated CO₂ also significantly increased the number of tillers or lateral branches produced by plants, which contributed to a significant increase in pod number and seed yield in these plants. Although plants grown at elevated CO₂ produced on average 8 seeds per pod, while plants grown under ambient CO2 conditions produced 5 seeds per pod, the greater number of seeds was offset by lower seed weights in plants grown under _ elevated CO₂. Thus, despite high seed yield in beans grown under elevated CO₂, the harvest index (HI) did not change significantly between CO₂ treatments.
- Full Text:
Essential fatty acids and ascorbic acid- interactions and effects on melanoma growth
- Authors: Gardiner, Neil Stockenstrom
- Date: 1990
- Subjects: Fatty acids , Melanoma , Mice -- Diseases
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4549 , http://hdl.handle.net/10962/d1018230
- Description: The present study was carried out to determine the effects and possible mechanisms of action of the essential fatty acids (EFAs) (linoleic acid (LA), gamma-linolenic acid (GLA) and arachidonic acid (AA)) and ascorbic acid (Asc) on BL6 murine melanoma growth in cell culture and in mice. Interactions between the nutrients in influencing melanoma growth as well as possible mechanisms of the interactions were also examined in the above systems. Cell culture studies revealed that all three EFAs (0-SOμg/ml) and Asc (0-200μg/ml) significantly inhibited melanoma growth at the concentrations used. The EF As were also found to significantly inhibit growth, although to a lesser extent than BL6 cells, of monkey kidney (LLCMK) cells which were used as a non-malignant control cell line. Asc in contrast was found not to inhibit growth of these cells. Supplementation of Asc (lOO)μg/ml) to EFA containing (0-50μg/ml) medium was found to significantly increase inhibition of cell growth in both cell lines, and in the BL6 cells in particular, after taking into account the growth inhibitory effects of Asc in the absence of EFAs. The mechanism of cell growth inhibition by the EF As appeared to involve lipid peroxidation but not enhanced prostaglandin (PG) or leukotriene (LT) synthesis. While Asc was found to increase both lipid peroxidation and PG synthesis in the cells, these mechanisms and enhanced LT synthesis did not appear to have played a role in the inhibition of cell growth by Asc or in the growth inhibitory interaction between Asc and the EF As. In vivo studies revealed that diets containing essential or polyunsaturated fatty acids (EFAs/PUFAs) in the form of vegetable oils, and in particular GLA in the form of evening primrose oil, significantly promoted melanoma growth in mice when compared with an EFA/PUFA free diet containing predominantly saturated fats (SF). Supplementary dietary Asc in contrast was found to significantly inhibit melanoma growth in mice fed EFA/PUFA, and in particular GLA, containing diets but not in mice fed SF cont~g diets. This result appears to indicate the occurrence of an interaction between the two nutrients. Ul The mechanism of tumour promotion by the EP As/PUP As did not appear to have involved enhanced PG or LT synthesis or lipid peroxidation. Since dietary EPA/PUPA manipulation was found to significantly alter the EPA content of tissues, including the melanomas, the mechanism of tumour promotion may have involved changes in the EPA composition of the tumour cells. While supplementary Asc was found to significantly increase the Asc content of certain tissues, including the melanomas, which may have played a role in tumour growth inhibition by Asc, it was found not to affect the EPA content of tissues. Enhanced PG or LT synthesis and lipid perox:idation did not appear to have been involved in the tumour growth inhibitory interaction between Asc and the EP As/PUP As. THe activity of the enzyme delta-6-desaturase, a key enzyme in EF A metabolism which catalyses the desaturation of LA to GLA, and the influence of Asc on activity of the enzyme were also examined. The cultured cells, and BL6 cells in particular, were found to contain significant activity of the enzyme. Whereas murine liver microsomal fractions were found to contain delta-6-desaturase activity, microsomes from melanomas grown in mice were found to lack activity of the enzyme. The significant tumour promoting effects of the GLA containing EPO diet may have been the result of the lack of delta-6-desaturase activity in tumour cells grown in mice. Asc was found to stimulate activity of the enzyme in cultured BL6 cells but not in LLCM.K cells, while dietary Asc and EF A/PUP A manipulation did not influence activity of the enzyme in microsomal fractions. This study has confirmed previous reports of the in vivo tumour promoting effects of dietary EP As/PUP As and the tumour growth inhibitory effects of Asc. The in vitro cell growth inhibitory effects of Asc and the EP As also confirm the results of previous reports. Previous studies investigating possible interactions between Asc and EP As/PUP As in influencing tumour cell growth could not be located in the relevant literature. This study may therefore be one of the first investigations of any such interaction between these nutrients in tumour cells. While this study was not able to identify the mechanisms involved in the different tumour promoting or tumour growth inhibitory effects of the two nutrients in the two systems, it did eliminate a number of potential mechanisms. The results of this study also emphasise the difficulty of attempting to compare the results of in vitro and in vivo studies.
- Full Text:
- Authors: Gardiner, Neil Stockenstrom
- Date: 1990
- Subjects: Fatty acids , Melanoma , Mice -- Diseases
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4549 , http://hdl.handle.net/10962/d1018230
- Description: The present study was carried out to determine the effects and possible mechanisms of action of the essential fatty acids (EFAs) (linoleic acid (LA), gamma-linolenic acid (GLA) and arachidonic acid (AA)) and ascorbic acid (Asc) on BL6 murine melanoma growth in cell culture and in mice. Interactions between the nutrients in influencing melanoma growth as well as possible mechanisms of the interactions were also examined in the above systems. Cell culture studies revealed that all three EFAs (0-SOμg/ml) and Asc (0-200μg/ml) significantly inhibited melanoma growth at the concentrations used. The EF As were also found to significantly inhibit growth, although to a lesser extent than BL6 cells, of monkey kidney (LLCMK) cells which were used as a non-malignant control cell line. Asc in contrast was found not to inhibit growth of these cells. Supplementation of Asc (lOO)μg/ml) to EFA containing (0-50μg/ml) medium was found to significantly increase inhibition of cell growth in both cell lines, and in the BL6 cells in particular, after taking into account the growth inhibitory effects of Asc in the absence of EFAs. The mechanism of cell growth inhibition by the EF As appeared to involve lipid peroxidation but not enhanced prostaglandin (PG) or leukotriene (LT) synthesis. While Asc was found to increase both lipid peroxidation and PG synthesis in the cells, these mechanisms and enhanced LT synthesis did not appear to have played a role in the inhibition of cell growth by Asc or in the growth inhibitory interaction between Asc and the EF As. In vivo studies revealed that diets containing essential or polyunsaturated fatty acids (EFAs/PUFAs) in the form of vegetable oils, and in particular GLA in the form of evening primrose oil, significantly promoted melanoma growth in mice when compared with an EFA/PUFA free diet containing predominantly saturated fats (SF). Supplementary dietary Asc in contrast was found to significantly inhibit melanoma growth in mice fed EFA/PUFA, and in particular GLA, containing diets but not in mice fed SF cont~g diets. This result appears to indicate the occurrence of an interaction between the two nutrients. Ul The mechanism of tumour promotion by the EP As/PUP As did not appear to have involved enhanced PG or LT synthesis or lipid peroxidation. Since dietary EPA/PUPA manipulation was found to significantly alter the EPA content of tissues, including the melanomas, the mechanism of tumour promotion may have involved changes in the EPA composition of the tumour cells. While supplementary Asc was found to significantly increase the Asc content of certain tissues, including the melanomas, which may have played a role in tumour growth inhibition by Asc, it was found not to affect the EPA content of tissues. Enhanced PG or LT synthesis and lipid perox:idation did not appear to have been involved in the tumour growth inhibitory interaction between Asc and the EP As/PUP As. THe activity of the enzyme delta-6-desaturase, a key enzyme in EF A metabolism which catalyses the desaturation of LA to GLA, and the influence of Asc on activity of the enzyme were also examined. The cultured cells, and BL6 cells in particular, were found to contain significant activity of the enzyme. Whereas murine liver microsomal fractions were found to contain delta-6-desaturase activity, microsomes from melanomas grown in mice were found to lack activity of the enzyme. The significant tumour promoting effects of the GLA containing EPO diet may have been the result of the lack of delta-6-desaturase activity in tumour cells grown in mice. Asc was found to stimulate activity of the enzyme in cultured BL6 cells but not in LLCM.K cells, while dietary Asc and EF A/PUP A manipulation did not influence activity of the enzyme in microsomal fractions. This study has confirmed previous reports of the in vivo tumour promoting effects of dietary EP As/PUP As and the tumour growth inhibitory effects of Asc. The in vitro cell growth inhibitory effects of Asc and the EP As also confirm the results of previous reports. Previous studies investigating possible interactions between Asc and EP As/PUP As in influencing tumour cell growth could not be located in the relevant literature. This study may therefore be one of the first investigations of any such interaction between these nutrients in tumour cells. While this study was not able to identify the mechanisms involved in the different tumour promoting or tumour growth inhibitory effects of the two nutrients in the two systems, it did eliminate a number of potential mechanisms. The results of this study also emphasise the difficulty of attempting to compare the results of in vitro and in vivo studies.
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