Correlations between the occurence of virulent helicobacter pylori strains and chronic disease conditions in dyspeptic patients hospitalized in Chris Hani Baragwanath Hospital, Gauteng Province, South Africa
- Authors: Mzukwa, Asisipho
- Date: 2018
- Subjects: Helicobacter pylori infections Helicobacter pylori Bacterial diseases
- Language: English
- Type: Thesis , Masters , Microbiology
- Identifier: http://hdl.handle.net/10353/10885 , vital:35905
- Description: Introduction: Individuals predisposed to different chronic disease conditions such as type 2 diabetes mellitus (T2DM), hypertension (HPT) and human immunodeficiency virus (HIV) might be susceptible to persistent H. pylori colonization, which overtime encourages infection with virulent strains. The presence of different types of H. pylori virulence factors especially cagA, dupA and various combinations of vacA subtypes (s1/m1, s2/m1 and s2m2) confer a higher risk of gastric disease development. Aim: This study seeks to recover H. pylori from gastric biopsy specimen from patients with different chronic disease conditions referred to the GIT unit at Chris Hani Baragwanath Academic Hospital, Soweto, and confirm them and further determine the presence of virulent H. pylori strains in patients with T2DM, HPT and HIV. Methods: Gastric biopsies were obtained from 101 patients with different chronic disease conditions after obtaining consent between August 2017 and February 2018. H. pylori was isolated on selective Colombia blood agar. Presumptive isolates were identified following standard microbiology and biochemical technique. These isolates were confirmed by polymerase chain reaction (PCR) targeting the glmM gene. The H. pylori positive samples were further screened for cagA, dupA and vacA genotypes. Results: H. pylori was recovered from 26 patients, of which 8 were patients with T2DM, 10 with HPT and 8 with HIV. There was no correlation between H. pylori infection and the chronic conditions, but the majority of H. pylori positive participant carried virulent H. pylori strains. The cagA and the dupA genes were more prevalent in T2DM and HPT patients, however the association between these genes and the 2 chronic conditions was not significant. The vacA subtypes common in hypertensive patients compared to the other conditions were the s1m1, s1m2, and s2m2 and the association between these vacA forms and hypertension was significant. Majority of HIV infected patients were found to carry non-virulent H. pylori strains. Conclusion: The prevalence of H. pylori among the general population of patients with T2DM, HPT and HIV was low. Although infected patients are found to be carrying virulent strains of H. pylori, there is no significant association established between the virulent strains and the chronic conditions. Furthermore, HIV-infected patients were found to be carrying non-virulent strains of H. pylori.
- Full Text:
- Date Issued: 2018
- Authors: Mzukwa, Asisipho
- Date: 2018
- Subjects: Helicobacter pylori infections Helicobacter pylori Bacterial diseases
- Language: English
- Type: Thesis , Masters , Microbiology
- Identifier: http://hdl.handle.net/10353/10885 , vital:35905
- Description: Introduction: Individuals predisposed to different chronic disease conditions such as type 2 diabetes mellitus (T2DM), hypertension (HPT) and human immunodeficiency virus (HIV) might be susceptible to persistent H. pylori colonization, which overtime encourages infection with virulent strains. The presence of different types of H. pylori virulence factors especially cagA, dupA and various combinations of vacA subtypes (s1/m1, s2/m1 and s2m2) confer a higher risk of gastric disease development. Aim: This study seeks to recover H. pylori from gastric biopsy specimen from patients with different chronic disease conditions referred to the GIT unit at Chris Hani Baragwanath Academic Hospital, Soweto, and confirm them and further determine the presence of virulent H. pylori strains in patients with T2DM, HPT and HIV. Methods: Gastric biopsies were obtained from 101 patients with different chronic disease conditions after obtaining consent between August 2017 and February 2018. H. pylori was isolated on selective Colombia blood agar. Presumptive isolates were identified following standard microbiology and biochemical technique. These isolates were confirmed by polymerase chain reaction (PCR) targeting the glmM gene. The H. pylori positive samples were further screened for cagA, dupA and vacA genotypes. Results: H. pylori was recovered from 26 patients, of which 8 were patients with T2DM, 10 with HPT and 8 with HIV. There was no correlation between H. pylori infection and the chronic conditions, but the majority of H. pylori positive participant carried virulent H. pylori strains. The cagA and the dupA genes were more prevalent in T2DM and HPT patients, however the association between these genes and the 2 chronic conditions was not significant. The vacA subtypes common in hypertensive patients compared to the other conditions were the s1m1, s1m2, and s2m2 and the association between these vacA forms and hypertension was significant. Majority of HIV infected patients were found to carry non-virulent H. pylori strains. Conclusion: The prevalence of H. pylori among the general population of patients with T2DM, HPT and HIV was low. Although infected patients are found to be carrying virulent strains of H. pylori, there is no significant association established between the virulent strains and the chronic conditions. Furthermore, HIV-infected patients were found to be carrying non-virulent strains of H. pylori.
- Full Text:
- Date Issued: 2018
The implications of landfill closure for waste reclaimers in rural local municipalities : the case of Fort Beaufort landfill, Raymond Mhlaba Local Municipality, Eastern Cape South Africa
- Authors: Ntongana, Kwasa
- Date: 2018
- Subjects: Refuse and refuse disposal Sanitary landfills Waste disposal sites -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPhil
- Identifier: http://hdl.handle.net/10353/6027 , vital:29482
- Description: Poverty and unemployment in the rural areas of the Eastern Cape is forcing thousands of vulnerable people into waste picking. There is growing recognition that waste re-claimers contribute to the local economy, to public health and to environmental sustainability. However, they often face low social status, tragic living and working conditions, and little support from local governments. The waste reclaimers of the Raymond Mhlaba Municipal area are unemployed, experiencing a poorer quality of life and feeling even more socially and economically excluded from the rest of society because of the “limitations” on landfill salvaging currently. Moreover, they have developed feelings of resentment and anger that have resulted in defiant acts of trespassing and theft. This social development study highlights the implications of landfill closure for waste salvagers in Raymond Mhlaba Local Municipality. Landfilling has been the main method for municipal waste disposal in Raymond Mhlaba Local Municipality. To combat the effects of uncontrolled release of landfill gases, the uncontrolled release of leachate that may affect water quality many landfills in the Eastern Cape are being considered for close down and for rehabilitation. To consider as well is the socio-economic backlog and anticipated population growth in the province, which means that landfill salvaging will continue to be an important source of revenue for the poor and homeless. This research used observation, semi-structured one-on-one interviewing, focus group interviewing and questionnaires as well as conducted a review of waste management policy-related documents and secondary data analysis in this qualitative research to analyse the social and the economic impacts. The research found that waste reclaimers in the Raymond Mhlaba Local Municipality area disagreed with the closure of the Fort Beaufort landfill because of what the researcher assumed to be a lack of general environmental awareness and because of their vulnerability to poverty. Research findings also highlight that waste reclamation can be considered as a viable option to minimize waste at landfills and waste reclaimers have shown an increasing interest and commitment to being trained formally to continue reclamation work. This study has suggested disadvantages in rural landfill closure, without feasible alternatives. Focus must move away from extending waste collection and closing down landfills and instead improve final disposal and initiating minimisation- recycling programs as part of their strategies through the formation of waste cooperatives. This research suggests formalizing and controlling waste recovery to cater for the immediate and most basic needs of the poor. The recommendations will hopefully influence thinking about waste management policy planning and implementation in general, and be of service to furthering Raymond Mhlaba Local Municipality achieve goals of sustainable employment creation and poverty alleviation.
- Full Text:
- Date Issued: 2018
- Authors: Ntongana, Kwasa
- Date: 2018
- Subjects: Refuse and refuse disposal Sanitary landfills Waste disposal sites -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPhil
- Identifier: http://hdl.handle.net/10353/6027 , vital:29482
- Description: Poverty and unemployment in the rural areas of the Eastern Cape is forcing thousands of vulnerable people into waste picking. There is growing recognition that waste re-claimers contribute to the local economy, to public health and to environmental sustainability. However, they often face low social status, tragic living and working conditions, and little support from local governments. The waste reclaimers of the Raymond Mhlaba Municipal area are unemployed, experiencing a poorer quality of life and feeling even more socially and economically excluded from the rest of society because of the “limitations” on landfill salvaging currently. Moreover, they have developed feelings of resentment and anger that have resulted in defiant acts of trespassing and theft. This social development study highlights the implications of landfill closure for waste salvagers in Raymond Mhlaba Local Municipality. Landfilling has been the main method for municipal waste disposal in Raymond Mhlaba Local Municipality. To combat the effects of uncontrolled release of landfill gases, the uncontrolled release of leachate that may affect water quality many landfills in the Eastern Cape are being considered for close down and for rehabilitation. To consider as well is the socio-economic backlog and anticipated population growth in the province, which means that landfill salvaging will continue to be an important source of revenue for the poor and homeless. This research used observation, semi-structured one-on-one interviewing, focus group interviewing and questionnaires as well as conducted a review of waste management policy-related documents and secondary data analysis in this qualitative research to analyse the social and the economic impacts. The research found that waste reclaimers in the Raymond Mhlaba Local Municipality area disagreed with the closure of the Fort Beaufort landfill because of what the researcher assumed to be a lack of general environmental awareness and because of their vulnerability to poverty. Research findings also highlight that waste reclamation can be considered as a viable option to minimize waste at landfills and waste reclaimers have shown an increasing interest and commitment to being trained formally to continue reclamation work. This study has suggested disadvantages in rural landfill closure, without feasible alternatives. Focus must move away from extending waste collection and closing down landfills and instead improve final disposal and initiating minimisation- recycling programs as part of their strategies through the formation of waste cooperatives. This research suggests formalizing and controlling waste recovery to cater for the immediate and most basic needs of the poor. The recommendations will hopefully influence thinking about waste management policy planning and implementation in general, and be of service to furthering Raymond Mhlaba Local Municipality achieve goals of sustainable employment creation and poverty alleviation.
- Full Text:
- Date Issued: 2018
The role of the CCMA and bargaining councils in labour dispute resolution
- Authors: Mkalipi, Thembinkosi
- Date: 2018
- Subjects: South Africa -- Commission for Conciliation, Mediation, and Arbitration , Dispute resolution (Law) -- South Africa Arbitration, Industrial -- South Africa Collective bargaining -- South Africa
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: http://hdl.handle.net/10948/32182 , vital:31977
- Description: A feature of collective bargaining is that it has a habit of ending in deadlock. However, in the event of a dispute, an aggrieved party is not free to call an immediate strike or lockout. The Labour Relations Act of 1956 and the Labour Relations Act of 1995 (“the LRA”) provided for a strict procedure to be followed in an event of a dispute. The law before 1995 regarded industrial action in the case of a dispute that did not follow the procedure as an illegal act attracting criminal sanction. The law then distinguished between a dispute of right and a dispute of interest. Like many countries, South Africa, has a very violent history regarding the resolution of labour disputes. This is illustrated by the force and brutality that was used to solve the 1913 white miners’ strike after martial law was declared. The reaction of the employers and the state was more brutal. The same violence and brutality were applied in the 1914 railway-workers strike and the 1946 African workers strike. The most important purpose of labour law is to ensure labour peace will prevail in the labour market and to regulate relations between employer and employee. Labour peace ensures that the economy functions effectively and if this happens, society at large benefits greatly. In Chapter 1 of the LRA one of the purposes of the LRA is stated as: “the effective resolution of labour disputes.” This section is intended to give effect section 23(1) of the Constitution, which provide that, ‘‘everyone has the right to fair labour practices’’1. This treatise will analyse the role of the Commission for Conciliation, Mediation and Arbitration (the CCMA) in dispute resolution; its functions and jurisdiction, ensure labour peace and whether the dispute resolution system provided in the law and implemented by the different dispute resolution institutions have succeeded to advance Constitution of the Republic of South Africa Act 108 [1996]. Economic development, social justice, labour peace and the democratisation of the workplace to fulfil the purpose of the LRA. Under the previous Labour Relations Act2 (the “1956 LRA”) disputes were resolved through three main institutions namely, Conciliation Boards, Industrial Councils and the Industrial Court3. A Conciliation Board was on ad hoc - body established by the Minister of Manpower on application by any one of the parties in dispute. There was no requirement for an agreement between the disputing parties to apply for the establishment of a Conciliation Board. Either party could approach the Minister for its establishment. This application could be made on the workers side by one or more registered trade unions, one or more employees, or one or more registered trade unions. On the employer’s side, the application can be made by one or more registered employers’ organisations or, one or more employers.
- Full Text:
- Date Issued: 2018
- Authors: Mkalipi, Thembinkosi
- Date: 2018
- Subjects: South Africa -- Commission for Conciliation, Mediation, and Arbitration , Dispute resolution (Law) -- South Africa Arbitration, Industrial -- South Africa Collective bargaining -- South Africa
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: http://hdl.handle.net/10948/32182 , vital:31977
- Description: A feature of collective bargaining is that it has a habit of ending in deadlock. However, in the event of a dispute, an aggrieved party is not free to call an immediate strike or lockout. The Labour Relations Act of 1956 and the Labour Relations Act of 1995 (“the LRA”) provided for a strict procedure to be followed in an event of a dispute. The law before 1995 regarded industrial action in the case of a dispute that did not follow the procedure as an illegal act attracting criminal sanction. The law then distinguished between a dispute of right and a dispute of interest. Like many countries, South Africa, has a very violent history regarding the resolution of labour disputes. This is illustrated by the force and brutality that was used to solve the 1913 white miners’ strike after martial law was declared. The reaction of the employers and the state was more brutal. The same violence and brutality were applied in the 1914 railway-workers strike and the 1946 African workers strike. The most important purpose of labour law is to ensure labour peace will prevail in the labour market and to regulate relations between employer and employee. Labour peace ensures that the economy functions effectively and if this happens, society at large benefits greatly. In Chapter 1 of the LRA one of the purposes of the LRA is stated as: “the effective resolution of labour disputes.” This section is intended to give effect section 23(1) of the Constitution, which provide that, ‘‘everyone has the right to fair labour practices’’1. This treatise will analyse the role of the Commission for Conciliation, Mediation and Arbitration (the CCMA) in dispute resolution; its functions and jurisdiction, ensure labour peace and whether the dispute resolution system provided in the law and implemented by the different dispute resolution institutions have succeeded to advance Constitution of the Republic of South Africa Act 108 [1996]. Economic development, social justice, labour peace and the democratisation of the workplace to fulfil the purpose of the LRA. Under the previous Labour Relations Act2 (the “1956 LRA”) disputes were resolved through three main institutions namely, Conciliation Boards, Industrial Councils and the Industrial Court3. A Conciliation Board was on ad hoc - body established by the Minister of Manpower on application by any one of the parties in dispute. There was no requirement for an agreement between the disputing parties to apply for the establishment of a Conciliation Board. Either party could approach the Minister for its establishment. This application could be made on the workers side by one or more registered trade unions, one or more employees, or one or more registered trade unions. On the employer’s side, the application can be made by one or more registered employers’ organisations or, one or more employers.
- Full Text:
- Date Issued: 2018
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