Chemical composition of leaf essential oils of Lantana camara varieties in South Africa and their effect on the behavioural preference of Falconia intermedia
- Authors: Ngxande-Koza, Samella W
- Date: 2017
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4877 , vital:20739
- Description: Lantana camara L. (Verbenaceae) is one the most problematic invaders in South Africa invading forest edges, sand dunes, and shorelines by forming impenetrable thickets. Lantana camara invasions degrade natural biodiversity, reduce the value of land and consequently it has been a target for biological control, over the last 50 years in South Africa. Studies that have reported on chemical profile of Lantana camara have been conducted around the world but not in South Africa. Hence, the first aim of the current study was to identify the chemical baseline of L. camara varieties in the Eastern Cape, South Africa. Recent studies have shown that feeding by one of the agents released against L. camara, Falconia intermedia (Distant) (Hemiptera: Miridae), induces anti-herbivory response through increased leaf toughness and trichome density. A preliminary study conducted also reported the production of volatile chemicals by one variety, Whitney Farm, due to feeding by the mirids. Therefore, the second aim was to determine the induced changes in chemical compounds of L. camara varieties after feeding by F. intermedia. A third aim was to determine the effect these chemical compounds have on the behaviour of F. intermedia. To identify the chemical baseline of L. camara varieties, the essential oils of four L. camara varieties (East London, Port Alfred, Whitney Farm and Heather Glen) were analysed using gas chromatography mass spectrometry (GC-MS) and that resulted to the identification of 163 constitutive and 75 induced chemicals across the varieties tested. Lantana camara varieties showed different chemical classes but were highly dominated by terpenes. A great variation in the number of constitutive chemical compounds was found in all the varieties. There were 56 constitutive chemical compounds in the Whitney Farm variety, 41 in the East London variety, 36 in the Heather Glen variety and 30 in the Port Alfred variety. The Whitney Farm variety had the highest number (22) of unique constitutive chemicals identified when compared with other varieties. This indicates the chemical distinctiveness of the Whitney Farm variety from the other varieties. In the varieties tested, there were common chemical compounds identified in constitutive and induced (discussed below) states of the plants such as caryophyllene, hexane, naphthalene, copaene and a-caryophyllene. Besides naphthalene, the majority of chemical compounds in South African L. camara varieties were similar to compounds that have been identified across the world, suggesting that they are closely related. The expression of naphthalene in these varieties may be due to changes in the chemicals expressed over evolutionary time as predicted by the Novel Weapons Hypothesis. Amongst the varieties, a great variation in chemical compounds and their concentrations was shown in the induced states of the plants. The concentration of constitutive caryophyllene ranged from (3.13 - 15.7) %, to (4.02 - 11.10) % after feeding. The concentration of constitutive hexane ranged from (6.13 - 71.19) %, to (33.3 - 75.8) % after feeding. The concentration of constitutive naphthalene ranged from (0.21 - 4.79) %, to (0.92 - 2.11) % after feeding. The concentration of constitutive copaene ranged from (0.57 - 1.57) %, to (1.20 - 2.72) %. Lastly, the concentration of constitutive a-caryophyllene ranged from (1.18 - 9.03) %, to (0.78 - 5.48) % after feeding. The changes in chemical concentrations in lantana varieties indicated that feeding by the mirid on L. camara varieties causes an induction by either reducing or increasing the chemical concentrations. To determine the effect of the identified compounds on the behaviour of F. intermedia adults, olfactometer bioassays were conducted using a Y-tube technique. A significantly higher proportion of F. intermedia were attracted to undamaged leaves over damaged leaves and purified air. Undamaged leaves attracted 52 % of F. intermedia from the East London variety, 62.5 % from the Port Alfred variety, 56 % from the Whitney Farm variety, 58 % from the Lyndhurst variety and 54.5 % from the Heather Glen variety in dual choice trials versus damaged leaves. Furthermore, a significantly higher proportion of F. intermedia were attracted to damaged leaves over purified air. Damaged leaves attracted 67 % of F. intermedia from the East London variety, 67 % from the Port Alfred variety, 65.9 % from the Whitney Farm variety, 65.3 % from the Heather Glen variety and 64.5 % from the Lyndhurst variety. Olfactometer bioassays were also conducted using purified standard compounds of four chemical compounds identified from essential oils, hexane was used as a positive control as it is reported to be an insect attractant in literature. Hexane was highly attractive to the mirids compared to three standard compounds caryophyllene, caryophyllene oxide and naphthalene at the rate of 80 %, 73 % and 80 %, respectively. The standard compounds tested against F. intermedia are major compounds contained by L. camara varieties and they have proven to have a repellent effect. This may indicate that after feeding by F. intermedia, the major compounds expressed by the plant varieties repel F. intermedia contributing to the invasiveness of this weed. The increased expression of hexane and caryophyllene after feeding may also indicate increased attraction to some insects, opening up the potential for third trophic level interactions in varieties where this is the case. This is the first study on the chemical composition of essential oils of L. camara in South Africa. Therefore, we recommend that where appropriate chemical profile studies of the invasive alien plants should be considered during host specificity testing, and the vital role of chemical compounds on agent-weed interactions must be taken into consideration with other factors before and after the biological control agents are released.
- Full Text:
- Authors: Ngxande-Koza, Samella W
- Date: 2017
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4877 , vital:20739
- Description: Lantana camara L. (Verbenaceae) is one the most problematic invaders in South Africa invading forest edges, sand dunes, and shorelines by forming impenetrable thickets. Lantana camara invasions degrade natural biodiversity, reduce the value of land and consequently it has been a target for biological control, over the last 50 years in South Africa. Studies that have reported on chemical profile of Lantana camara have been conducted around the world but not in South Africa. Hence, the first aim of the current study was to identify the chemical baseline of L. camara varieties in the Eastern Cape, South Africa. Recent studies have shown that feeding by one of the agents released against L. camara, Falconia intermedia (Distant) (Hemiptera: Miridae), induces anti-herbivory response through increased leaf toughness and trichome density. A preliminary study conducted also reported the production of volatile chemicals by one variety, Whitney Farm, due to feeding by the mirids. Therefore, the second aim was to determine the induced changes in chemical compounds of L. camara varieties after feeding by F. intermedia. A third aim was to determine the effect these chemical compounds have on the behaviour of F. intermedia. To identify the chemical baseline of L. camara varieties, the essential oils of four L. camara varieties (East London, Port Alfred, Whitney Farm and Heather Glen) were analysed using gas chromatography mass spectrometry (GC-MS) and that resulted to the identification of 163 constitutive and 75 induced chemicals across the varieties tested. Lantana camara varieties showed different chemical classes but were highly dominated by terpenes. A great variation in the number of constitutive chemical compounds was found in all the varieties. There were 56 constitutive chemical compounds in the Whitney Farm variety, 41 in the East London variety, 36 in the Heather Glen variety and 30 in the Port Alfred variety. The Whitney Farm variety had the highest number (22) of unique constitutive chemicals identified when compared with other varieties. This indicates the chemical distinctiveness of the Whitney Farm variety from the other varieties. In the varieties tested, there were common chemical compounds identified in constitutive and induced (discussed below) states of the plants such as caryophyllene, hexane, naphthalene, copaene and a-caryophyllene. Besides naphthalene, the majority of chemical compounds in South African L. camara varieties were similar to compounds that have been identified across the world, suggesting that they are closely related. The expression of naphthalene in these varieties may be due to changes in the chemicals expressed over evolutionary time as predicted by the Novel Weapons Hypothesis. Amongst the varieties, a great variation in chemical compounds and their concentrations was shown in the induced states of the plants. The concentration of constitutive caryophyllene ranged from (3.13 - 15.7) %, to (4.02 - 11.10) % after feeding. The concentration of constitutive hexane ranged from (6.13 - 71.19) %, to (33.3 - 75.8) % after feeding. The concentration of constitutive naphthalene ranged from (0.21 - 4.79) %, to (0.92 - 2.11) % after feeding. The concentration of constitutive copaene ranged from (0.57 - 1.57) %, to (1.20 - 2.72) %. Lastly, the concentration of constitutive a-caryophyllene ranged from (1.18 - 9.03) %, to (0.78 - 5.48) % after feeding. The changes in chemical concentrations in lantana varieties indicated that feeding by the mirid on L. camara varieties causes an induction by either reducing or increasing the chemical concentrations. To determine the effect of the identified compounds on the behaviour of F. intermedia adults, olfactometer bioassays were conducted using a Y-tube technique. A significantly higher proportion of F. intermedia were attracted to undamaged leaves over damaged leaves and purified air. Undamaged leaves attracted 52 % of F. intermedia from the East London variety, 62.5 % from the Port Alfred variety, 56 % from the Whitney Farm variety, 58 % from the Lyndhurst variety and 54.5 % from the Heather Glen variety in dual choice trials versus damaged leaves. Furthermore, a significantly higher proportion of F. intermedia were attracted to damaged leaves over purified air. Damaged leaves attracted 67 % of F. intermedia from the East London variety, 67 % from the Port Alfred variety, 65.9 % from the Whitney Farm variety, 65.3 % from the Heather Glen variety and 64.5 % from the Lyndhurst variety. Olfactometer bioassays were also conducted using purified standard compounds of four chemical compounds identified from essential oils, hexane was used as a positive control as it is reported to be an insect attractant in literature. Hexane was highly attractive to the mirids compared to three standard compounds caryophyllene, caryophyllene oxide and naphthalene at the rate of 80 %, 73 % and 80 %, respectively. The standard compounds tested against F. intermedia are major compounds contained by L. camara varieties and they have proven to have a repellent effect. This may indicate that after feeding by F. intermedia, the major compounds expressed by the plant varieties repel F. intermedia contributing to the invasiveness of this weed. The increased expression of hexane and caryophyllene after feeding may also indicate increased attraction to some insects, opening up the potential for third trophic level interactions in varieties where this is the case. This is the first study on the chemical composition of essential oils of L. camara in South Africa. Therefore, we recommend that where appropriate chemical profile studies of the invasive alien plants should be considered during host specificity testing, and the vital role of chemical compounds on agent-weed interactions must be taken into consideration with other factors before and after the biological control agents are released.
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An assessment of the efficiency and effectiveness of the Working for Water NBAL mapping, contract teams and clearing of Acacia mearnsii
- Authors: Modipa, Rebone Dineo
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3192 , vital:20383
- Description: Working for Water (WfW) works to fulfil their dual mandate of protecting ecosystem services and creating employment opportunities for poor communities. There have been many successes but evidence is increasing regarding the inefficiencies at project and site scales. The study was undertaken in the Eastern Cape, South Africa, at a farm called Ann’s Villa situated in the Kommadagga valley, at the foot of the northern side of the Zuurberg pass. The study sought to assess the efficiency of the WfW mapping of natural biological aliens (NBAL), of contract teams and the effectiveness of the clearing methods employed to clear Acacia mearnsii. To achieve this, WfW mapping was analysed, contract team dynamics of the different task groups were observed and assessed and various Acacia mearnsii and indigenous plant variables were measured pre-and post-clearing of A. mearnsii. The first key finding was that overall the WfW NBAL mapping was inaccurate, with WfW A. mearnsii percentage cover (cover) estimations significantly over-estimated. Acacia mearnsii cover over-estimations resulted in fruitless expenditure as contracts were more expensive than necessary. Cover under-estimations also led to the inability to fulfill contractual obligations and the subsequent halting of the clearing of a large portion the study area. It was recommended that the NBAL mapping as well as the cover estimations of NBALs be conducted more rigorously, with expert assistance where skills are lacking, to avoid the associated fruitless expenditure. The second key finding was that WfW contract teams were largely inefficient, as a result of waiting, stemming from the lack of continuity in work. There was a strong positive relationship between subgroup chainsaw operator (CO) to stacker ratio and subgroup CO to stacker efficiency ratio. These inefficiencies meant that a mean of 58±67% of the total money spent per team resulted in fruitless expenditure. It was recommend that contract teams be organised to promote the continuity of work and that the current high stacker to CO ratios be reversed to increase efficiency and reduce fruitless expenditures. The third key finding was that overall the WfW methods of clearing A. mearnsii were 95% effective with a 90% removal success. The last key finding was that initial clearing operations had a largely insignificant impact on indigenous plant biodiversity. It was recommended that post-clearing inspection of quality control is conducted more rigorously and that penalties be implemented to deter contract teams from cutting indigenous species.
- Full Text:
- Authors: Modipa, Rebone Dineo
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3192 , vital:20383
- Description: Working for Water (WfW) works to fulfil their dual mandate of protecting ecosystem services and creating employment opportunities for poor communities. There have been many successes but evidence is increasing regarding the inefficiencies at project and site scales. The study was undertaken in the Eastern Cape, South Africa, at a farm called Ann’s Villa situated in the Kommadagga valley, at the foot of the northern side of the Zuurberg pass. The study sought to assess the efficiency of the WfW mapping of natural biological aliens (NBAL), of contract teams and the effectiveness of the clearing methods employed to clear Acacia mearnsii. To achieve this, WfW mapping was analysed, contract team dynamics of the different task groups were observed and assessed and various Acacia mearnsii and indigenous plant variables were measured pre-and post-clearing of A. mearnsii. The first key finding was that overall the WfW NBAL mapping was inaccurate, with WfW A. mearnsii percentage cover (cover) estimations significantly over-estimated. Acacia mearnsii cover over-estimations resulted in fruitless expenditure as contracts were more expensive than necessary. Cover under-estimations also led to the inability to fulfill contractual obligations and the subsequent halting of the clearing of a large portion the study area. It was recommended that the NBAL mapping as well as the cover estimations of NBALs be conducted more rigorously, with expert assistance where skills are lacking, to avoid the associated fruitless expenditure. The second key finding was that WfW contract teams were largely inefficient, as a result of waiting, stemming from the lack of continuity in work. There was a strong positive relationship between subgroup chainsaw operator (CO) to stacker ratio and subgroup CO to stacker efficiency ratio. These inefficiencies meant that a mean of 58±67% of the total money spent per team resulted in fruitless expenditure. It was recommend that contract teams be organised to promote the continuity of work and that the current high stacker to CO ratios be reversed to increase efficiency and reduce fruitless expenditures. The third key finding was that overall the WfW methods of clearing A. mearnsii were 95% effective with a 90% removal success. The last key finding was that initial clearing operations had a largely insignificant impact on indigenous plant biodiversity. It was recommended that post-clearing inspection of quality control is conducted more rigorously and that penalties be implemented to deter contract teams from cutting indigenous species.
- Full Text:
Statistical analysis of the ionospheric response during storm conditions over South Africa using ionosonde and GPS data
- Matamba, Tshimangadzo Merline
- Authors: Matamba, Tshimangadzo Merline
- Date: 2015
- Subjects: Ionospheric storms -- South Africa -- Grahamstown , Ionospheric storms -- South Africa -- Madimbo , Magnetic storms -- South Africa -- Grahamstown , Magnetic storms -- South Africa -- Madimbo , Ionosondes , Global Positioning System
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5555 , http://hdl.handle.net/10962/d1017899
- Description: Ionospheric storms are an extreme form of space weather phenomena which affect space- and ground-based technological systems. Extreme solar activity may give rise to Coronal Mass Ejections (CME) and solar flares that may result in ionospheric storms. This thesis reports on a statistical analysis of the ionospheric response over the ionosonde stations Grahamstown (33.3◦S, 26.5◦E) and Madimbo (22.4◦S,30.9◦E), South Africa, during geomagnetic storm conditions which occurred during the period 1996 - 2011. Total Electron Content (TEC) derived from Global Positioning System (GPS) data by a dual Frequency receiver and an ionosonde at Grahamstown, was analysed for the storms that occurred during the period 2006 - 2011. A comprehensive analysis of the critical frequency of the F2 layer (foF2) and TEC was done. To identify the geomagnetically disturbed conditions the Disturbance storm time (Dst) index with a storm criteria of Dst ≤ −50 nT was used. The ionospheric disturbances were categorized into three responses, namely single disturbance, double disturbance and not significant (NS) ionospheric storms. Single disturbance ionospheric storms refer to positive (P) and negative (N) ionospheric storms observed separately, while double disturbance storms refer to negative and positive ionospheric storms observed during the same storm period. The statistics show the impact of geomagnetic storms on the ionosphere and indicate that negative ionospheric effects follow the solar cycle. In general, only a few ionospheric storms (0.11%) were observed during solar minimum. Positive ionospheric storms occurred most frequently (47.54%) during the declining phase of solar cycle 23. Seasonally, negative ionospheric storms occurred mostly during the summer (63.24%), while positive ionospheric storms occurred frequently during the winter (53.62%). An important finding is that only negative ionospheric storms were observed during great geomagnetic storm activity (Dst ≤ −350 nT). For periods when both ionosonde and GPS was available, the two data sets indicated similar ionospheric responses. Hence, GPS data can be used to effectively identify the ionospheric response in the absence of ionosonde data.
- Full Text:
- Authors: Matamba, Tshimangadzo Merline
- Date: 2015
- Subjects: Ionospheric storms -- South Africa -- Grahamstown , Ionospheric storms -- South Africa -- Madimbo , Magnetic storms -- South Africa -- Grahamstown , Magnetic storms -- South Africa -- Madimbo , Ionosondes , Global Positioning System
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5555 , http://hdl.handle.net/10962/d1017899
- Description: Ionospheric storms are an extreme form of space weather phenomena which affect space- and ground-based technological systems. Extreme solar activity may give rise to Coronal Mass Ejections (CME) and solar flares that may result in ionospheric storms. This thesis reports on a statistical analysis of the ionospheric response over the ionosonde stations Grahamstown (33.3◦S, 26.5◦E) and Madimbo (22.4◦S,30.9◦E), South Africa, during geomagnetic storm conditions which occurred during the period 1996 - 2011. Total Electron Content (TEC) derived from Global Positioning System (GPS) data by a dual Frequency receiver and an ionosonde at Grahamstown, was analysed for the storms that occurred during the period 2006 - 2011. A comprehensive analysis of the critical frequency of the F2 layer (foF2) and TEC was done. To identify the geomagnetically disturbed conditions the Disturbance storm time (Dst) index with a storm criteria of Dst ≤ −50 nT was used. The ionospheric disturbances were categorized into three responses, namely single disturbance, double disturbance and not significant (NS) ionospheric storms. Single disturbance ionospheric storms refer to positive (P) and negative (N) ionospheric storms observed separately, while double disturbance storms refer to negative and positive ionospheric storms observed during the same storm period. The statistics show the impact of geomagnetic storms on the ionosphere and indicate that negative ionospheric effects follow the solar cycle. In general, only a few ionospheric storms (0.11%) were observed during solar minimum. Positive ionospheric storms occurred most frequently (47.54%) during the declining phase of solar cycle 23. Seasonally, negative ionospheric storms occurred mostly during the summer (63.24%), while positive ionospheric storms occurred frequently during the winter (53.62%). An important finding is that only negative ionospheric storms were observed during great geomagnetic storm activity (Dst ≤ −350 nT). For periods when both ionosonde and GPS was available, the two data sets indicated similar ionospheric responses. Hence, GPS data can be used to effectively identify the ionospheric response in the absence of ionosonde data.
- Full Text:
Geographical variation in effects of nutrient levels and grazing intensity on community structure between upwelling and non-upwelling regions of South Africa
- Authors: Steele, Nikita
- Date: 2014
- Subjects: Upwelling (Oceanography) , Upwelling (Oceanography) -- Indian Ocean , Algae -- Indian Ocean , Algae -- Effect of grazing on -- Indian Ocean
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5873 , http://hdl.handle.net/10962/d1013013
- Description: The aim of this thesis was to assess the influence of upwelling on alga-grazer interactions in rocky shore communities along the south coast of South Africa using grazer exclusion treatments with controls and procedural controls set out in a block design and monitored for algal cover roughly monthly for one year. In the first experiment grazers were excluded from treatment plots at two upwelling and two non-upwelling sites and the rates of algal biomass accumulation were then compared. The upwelling sites showed significantly faster algal colonisation rates, with Ulva rigida being the first species to colonise the rocks. Final algal cover and biomass did not differ significantly between upwelling and non-upwelling sites in control plots open to grazers, but were significantly higher in grazer exclusion plots at upwelling sites indicating stronger grazing effects. This was confirmed by estimating the intensity of grazing using the log-response ratio (LRR), which was calculated from treatment and control plots. Upwelling sites had significantly lower LLR values indicating stronger grazing effects, than at non-upwelling sites, despite no difference in grazer abundances. The second experiment examined the effects of nutrient addition on algal growth and community composition by comparing high nutrient enrichment plots with low enrichment plots at one upwelling and one non-upwelling site. ANOVA indicated faster growth rates and significantly higher final algal biomass in high enrichment plots compared to low enrichment and control plots at both upwelling and non-upwelling sites. A two-way ANOVA indicated significantly higher algal cover in high enrichment plots compared to the data from the grazer exclusion plots in experiment 1 at both sites, suggesting that nutrient addition plays a major role in algal growth and community composition. The findings of these studies have shown significant differences between treatments, sites and seasons, with significant differences not only occurring in algal cover but also accumulation of algal biomass and recruitment patterns between treatments. The small scale local processes acting within a few centimetres (plots) or tens of meters (among blocks) can also be reflected over larger scales such as sites (upwelling/non-upwelling shores). Further, these studies have demonstrated that various factors such as the effects from increased nutrients at upwelling cells and the change in grazing effects due to enhanced nutrients can determine the abundance and diversity of the community structure, including an increase in the abundance of the fast growing algae Ulva rigida, and a slow recovery of the brown and red algae.
- Full Text:
- Authors: Steele, Nikita
- Date: 2014
- Subjects: Upwelling (Oceanography) , Upwelling (Oceanography) -- Indian Ocean , Algae -- Indian Ocean , Algae -- Effect of grazing on -- Indian Ocean
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5873 , http://hdl.handle.net/10962/d1013013
- Description: The aim of this thesis was to assess the influence of upwelling on alga-grazer interactions in rocky shore communities along the south coast of South Africa using grazer exclusion treatments with controls and procedural controls set out in a block design and monitored for algal cover roughly monthly for one year. In the first experiment grazers were excluded from treatment plots at two upwelling and two non-upwelling sites and the rates of algal biomass accumulation were then compared. The upwelling sites showed significantly faster algal colonisation rates, with Ulva rigida being the first species to colonise the rocks. Final algal cover and biomass did not differ significantly between upwelling and non-upwelling sites in control plots open to grazers, but were significantly higher in grazer exclusion plots at upwelling sites indicating stronger grazing effects. This was confirmed by estimating the intensity of grazing using the log-response ratio (LRR), which was calculated from treatment and control plots. Upwelling sites had significantly lower LLR values indicating stronger grazing effects, than at non-upwelling sites, despite no difference in grazer abundances. The second experiment examined the effects of nutrient addition on algal growth and community composition by comparing high nutrient enrichment plots with low enrichment plots at one upwelling and one non-upwelling site. ANOVA indicated faster growth rates and significantly higher final algal biomass in high enrichment plots compared to low enrichment and control plots at both upwelling and non-upwelling sites. A two-way ANOVA indicated significantly higher algal cover in high enrichment plots compared to the data from the grazer exclusion plots in experiment 1 at both sites, suggesting that nutrient addition plays a major role in algal growth and community composition. The findings of these studies have shown significant differences between treatments, sites and seasons, with significant differences not only occurring in algal cover but also accumulation of algal biomass and recruitment patterns between treatments. The small scale local processes acting within a few centimetres (plots) or tens of meters (among blocks) can also be reflected over larger scales such as sites (upwelling/non-upwelling shores). Further, these studies have demonstrated that various factors such as the effects from increased nutrients at upwelling cells and the change in grazing effects due to enhanced nutrients can determine the abundance and diversity of the community structure, including an increase in the abundance of the fast growing algae Ulva rigida, and a slow recovery of the brown and red algae.
- Full Text:
Confirmation of a slow symplasmic loading and unloading pathway in barley (Hordeum Vulgare L.) source and sink leaves
- Authors: Buwa, Lisa Valencia
- Date: 2003
- Subjects: Phloem , Plant translocation , Barley -- Metabolism
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4253 , http://hdl.handle.net/10962/d1007804
- Description: Visualization of the transport pathway in barley (Hordeum vulgare) leaves was carried out using a combination of aniline blue and a symplasmically transported fluorochrome, 5,6 carboxyfluorescein (5,6-CF). When applied to a source leaf, basipetal movement of 5,6-CF was observed after 3 h and the fluorochrome front was observed about 3-4cm away from the point of application. The fluorochrome was taken up into the symplasm of the mesophyll and was loaded into the bundle sheath cells and then subsequently the vascular parenchyma and finally into the sieve tubes. In sink leaves, acropetal movement was observed after 3 h and the fluorochrome had moved approximately 3 cm away from the point of application. Unloading of 5,6-CF occurred from all classes of longitudinal veins. Studies on solute retrieval showed that 5,6 CF-diacetate was transferred to xylem parenchyma where it was metabolized. 5,6-CF was then transferred from the xylem parenchyma to the vascular parenchyma cells, and it would appear that thick-walled sieve tubes were the first to show 5,6-CF labeling. Counterstaining with aniline blue demonstrates the presence of plasmodesmata and this suggests a potential symplasmic pathway from the mesophyll to the sieve tubes. Application of 5,6 CF-diacetate revealed a slow symplasmic pathway, which involved transfer of 5,6-CF, which was effected via plasmodesma.
- Full Text:
- Authors: Buwa, Lisa Valencia
- Date: 2003
- Subjects: Phloem , Plant translocation , Barley -- Metabolism
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4253 , http://hdl.handle.net/10962/d1007804
- Description: Visualization of the transport pathway in barley (Hordeum vulgare) leaves was carried out using a combination of aniline blue and a symplasmically transported fluorochrome, 5,6 carboxyfluorescein (5,6-CF). When applied to a source leaf, basipetal movement of 5,6-CF was observed after 3 h and the fluorochrome front was observed about 3-4cm away from the point of application. The fluorochrome was taken up into the symplasm of the mesophyll and was loaded into the bundle sheath cells and then subsequently the vascular parenchyma and finally into the sieve tubes. In sink leaves, acropetal movement was observed after 3 h and the fluorochrome had moved approximately 3 cm away from the point of application. Unloading of 5,6-CF occurred from all classes of longitudinal veins. Studies on solute retrieval showed that 5,6 CF-diacetate was transferred to xylem parenchyma where it was metabolized. 5,6-CF was then transferred from the xylem parenchyma to the vascular parenchyma cells, and it would appear that thick-walled sieve tubes were the first to show 5,6-CF labeling. Counterstaining with aniline blue demonstrates the presence of plasmodesmata and this suggests a potential symplasmic pathway from the mesophyll to the sieve tubes. Application of 5,6 CF-diacetate revealed a slow symplasmic pathway, which involved transfer of 5,6-CF, which was effected via plasmodesma.
- Full Text:
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