An exploration into the meaning of death
- Authors: Todres, Leslie Allen
- Date: 1980 , 2013-03-28
- Subjects: Death -- Psychological aspects
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3107 , http://hdl.handle.net/10962/d1004519 , Death -- Psychological aspects
- Description: From Preface: The present study attempts to explore, both theoretically and empirically, some implications that a personal consideration of death may have for the way we live our lives. Existential-Phenomenology has concerned itself with the description of the dimensions of human existence. It is in this enquiry that Death, and through it, human finitude, emerges as an important dimension of existence. It is not the fact of death itself, but the MEANING it has for our lives which may be of important consequence. It is to this area that this research addresses itself. The methodology employed arises out of a concern to contribute to the establishment of Psychology as a Human Science. To this end, a chapter focusing on the philosophical bases of methodology is included. It is hoped that this study may, however little, contribute both to the field of Thanatology as well as to the more theoretical issue of what Psychology is, and where it is going. , KMBT_363 , Adobe Acrobat 9.53 Paper Capture Plug-in
- Full Text:
- Date Issued: 1980
- Authors: Todres, Leslie Allen
- Date: 1980 , 2013-03-28
- Subjects: Death -- Psychological aspects
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3107 , http://hdl.handle.net/10962/d1004519 , Death -- Psychological aspects
- Description: From Preface: The present study attempts to explore, both theoretically and empirically, some implications that a personal consideration of death may have for the way we live our lives. Existential-Phenomenology has concerned itself with the description of the dimensions of human existence. It is in this enquiry that Death, and through it, human finitude, emerges as an important dimension of existence. It is not the fact of death itself, but the MEANING it has for our lives which may be of important consequence. It is to this area that this research addresses itself. The methodology employed arises out of a concern to contribute to the establishment of Psychology as a Human Science. To this end, a chapter focusing on the philosophical bases of methodology is included. It is hoped that this study may, however little, contribute both to the field of Thanatology as well as to the more theoretical issue of what Psychology is, and where it is going. , KMBT_363 , Adobe Acrobat 9.53 Paper Capture Plug-in
- Full Text:
- Date Issued: 1980
An investigation into nineteenth century book illustration with reference to the movement towards total collaboration in the works of Charles Dickens and his major illustrators
- Authors: Crowe, Richard J J
- Date: 1980
- Subjects: Dickens, Charles, 1812-1870 -- Illustrations Illustrators llustration of books -- Great Britain -- 19th century
- Language: English
- Type: Thesis , Masters , MFA
- Identifier: vital:2467 , http://hdl.handle.net/10962/d1009433
- Description: It is necessary to state clearly at the outset of this essay that the field comprising Book Illustration is extremely complex. This is not difficult to appreciate when one considers that the term "Book Illustration" covers both the highly developed art forms such as are found for example in the Book of Kells; and at the same time such cheap and shallow examples as are found in magazines and books that are churned out for the million. Therefore it becomes necessary to draw a sharp distinction between what could be called "inferior" and "superior" quality of Book Illustration. (a) I see this dividing line being drawn between two fundamentally divergent attitudes: the one involves a purely external and decorative approach which lacks real artistic value; (b) and the other, which is the result of the dynamic collaboration between an author and an artist to produce work founded on deep and rich artistic principles. (c) For the sake of clarity I wish to take this idea of a division a step further, and to suggest that within the "superior" bracket there is also a continuing scale of improvement and bettering, which culminates in an idealised state which could be called "TOTAL COLLABORATION" between an author and an artist.
- Full Text:
- Date Issued: 1980
- Authors: Crowe, Richard J J
- Date: 1980
- Subjects: Dickens, Charles, 1812-1870 -- Illustrations Illustrators llustration of books -- Great Britain -- 19th century
- Language: English
- Type: Thesis , Masters , MFA
- Identifier: vital:2467 , http://hdl.handle.net/10962/d1009433
- Description: It is necessary to state clearly at the outset of this essay that the field comprising Book Illustration is extremely complex. This is not difficult to appreciate when one considers that the term "Book Illustration" covers both the highly developed art forms such as are found for example in the Book of Kells; and at the same time such cheap and shallow examples as are found in magazines and books that are churned out for the million. Therefore it becomes necessary to draw a sharp distinction between what could be called "inferior" and "superior" quality of Book Illustration. (a) I see this dividing line being drawn between two fundamentally divergent attitudes: the one involves a purely external and decorative approach which lacks real artistic value; (b) and the other, which is the result of the dynamic collaboration between an author and an artist to produce work founded on deep and rich artistic principles. (c) For the sake of clarity I wish to take this idea of a division a step further, and to suggest that within the "superior" bracket there is also a continuing scale of improvement and bettering, which culminates in an idealised state which could be called "TOTAL COLLABORATION" between an author and an artist.
- Full Text:
- Date Issued: 1980
An investigation of the corporate planning concept in a South African motor manufacturing undertaking
- Authors: Coman, Keith Rawson
- Date: 1980
- Subjects: Business planning -- South Africa , Motor vehicle industry -- South Africa
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1081 , http://hdl.handle.net/10962/d1010583 , Business planning -- South Africa , Motor vehicle industry -- South Africa
- Description: The intention of this dissertation is to attempt to project the theoretical concept of Corporate (or Long-Range) Planning into the reality of one of South Africa's major motor vehicle manufacturing company's task environment. It is a sad fact that there are no universally - applicable practical planning "laws" (as are present within the discipline of, say, Physics) amidst the ever-changing hurly-burly world of modern commerce and industry. Accordingly, there are very real distinctions between distilled Corporate Planning theory, as it is propounded in the many available books and articles, and Corporate Planning practice as actually implemented. This is true largely by virtue of the fact that in the same way as persons differ in their physical and mental make-up, so too do individual companies; even to the extent of acquiring a distinct corporate "personality" of their own. Terms of reference, p. 1.
- Full Text:
- Date Issued: 1980
- Authors: Coman, Keith Rawson
- Date: 1980
- Subjects: Business planning -- South Africa , Motor vehicle industry -- South Africa
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1081 , http://hdl.handle.net/10962/d1010583 , Business planning -- South Africa , Motor vehicle industry -- South Africa
- Description: The intention of this dissertation is to attempt to project the theoretical concept of Corporate (or Long-Range) Planning into the reality of one of South Africa's major motor vehicle manufacturing company's task environment. It is a sad fact that there are no universally - applicable practical planning "laws" (as are present within the discipline of, say, Physics) amidst the ever-changing hurly-burly world of modern commerce and industry. Accordingly, there are very real distinctions between distilled Corporate Planning theory, as it is propounded in the many available books and articles, and Corporate Planning practice as actually implemented. This is true largely by virtue of the fact that in the same way as persons differ in their physical and mental make-up, so too do individual companies; even to the extent of acquiring a distinct corporate "personality" of their own. Terms of reference, p. 1.
- Full Text:
- Date Issued: 1980
Aspects of the theory and practice of choral training in South African schools
- Authors: Radue, Robin R
- Date: 1980
- Subjects: Choral music -- Scores Choral music -- Instruction and study Choirs (Music) -- Instruction and study , Choral singing , Scores , Children's choirs , Choral training
- Language: English
- Type: Thesis , Masters , MMus
- Identifier: vital:2629 , http://hdl.handle.net/10962/d1001974
- Description: Firstly, the matter of constituting a choir is discussed. This involves auditioning, with its attendant problems of nervousness and self-consciousness on the part of the children, and assessment on that of the trainer. In the final analysis, after enthusiastically advertising the rewards to be gained from choral singing, and after trying to create an environment which is seen to be conducive to this philosophy, the trainer has to make the best of the willing forces available. Rehearsals are probably more important than performances, since the time devoted to preparation is much longer. Moreover, the educational value of carefully preparing the music and of becoming acquainted with the techniques of singing and with the aesthetic niceties of style and interpretation should by no means be underestimated. The choir and trainer get to know not only the music but also each other, and the trainer has to use his skills wisely in developing the human and musical resources available to him, in order to realize their greatest potential (Introduction, p. ii-iii)
- Full Text:
- Date Issued: 1980
- Authors: Radue, Robin R
- Date: 1980
- Subjects: Choral music -- Scores Choral music -- Instruction and study Choirs (Music) -- Instruction and study , Choral singing , Scores , Children's choirs , Choral training
- Language: English
- Type: Thesis , Masters , MMus
- Identifier: vital:2629 , http://hdl.handle.net/10962/d1001974
- Description: Firstly, the matter of constituting a choir is discussed. This involves auditioning, with its attendant problems of nervousness and self-consciousness on the part of the children, and assessment on that of the trainer. In the final analysis, after enthusiastically advertising the rewards to be gained from choral singing, and after trying to create an environment which is seen to be conducive to this philosophy, the trainer has to make the best of the willing forces available. Rehearsals are probably more important than performances, since the time devoted to preparation is much longer. Moreover, the educational value of carefully preparing the music and of becoming acquainted with the techniques of singing and with the aesthetic niceties of style and interpretation should by no means be underestimated. The choir and trainer get to know not only the music but also each other, and the trainer has to use his skills wisely in developing the human and musical resources available to him, in order to realize their greatest potential (Introduction, p. ii-iii)
- Full Text:
- Date Issued: 1980
Assessment of some variables affecting the blanching activity of betamethasone 17-valerate cream
- Magnus, Ashley D, Haigh, John M, Kanfer, Isadore
- Authors: Magnus, Ashley D , Haigh, John M , Kanfer, Isadore
- Date: 1980
- Language: English
- Type: text , Article
- Identifier: vital:6396 , http://hdl.handle.net/10962/d1006320
- Description: The effect of concentration and occlusion time on the ability of Betnovate ® cream (betamethasone 17-valerate 0.1%) to produce skin blanching was assessed. Generally, increased concentration or occlusion time produce and increase in the degree of blanching observed, however, a plateau stage is eventually reached where no further increase of blanching occurs.
- Full Text:
- Date Issued: 1980
- Authors: Magnus, Ashley D , Haigh, John M , Kanfer, Isadore
- Date: 1980
- Language: English
- Type: text , Article
- Identifier: vital:6396 , http://hdl.handle.net/10962/d1006320
- Description: The effect of concentration and occlusion time on the ability of Betnovate ® cream (betamethasone 17-valerate 0.1%) to produce skin blanching was assessed. Generally, increased concentration or occlusion time produce and increase in the degree of blanching observed, however, a plateau stage is eventually reached where no further increase of blanching occurs.
- Full Text:
- Date Issued: 1980
Bacteriophage growth on stationary phase achromabacter strains
- Authors: Robb, Susan Mary
- Date: 1980
- Subjects: Bacteriophages , Strains and stresses
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4125 , http://hdl.handle.net/10962/d1014131
- Description: Achromobacter w.t. and strain 14 both support phage α3a growth in stationary phase, but unlike the w.t. strain, exponential phase cultures of strain 14 block phage development. A standard method was developed for determining phage growth in stationary phase cultures. Lyophilised cells were used to eliminate variations due to the unstable phenotype of Achromobacter strain 14 cells. Phage α3a growth in stationary phase was characterized by a long and variable latent period of 6 to 9 h and an increased burst size of 709 p.f.u. per cell as compared with 153 p.f.u. per cell in exponential wild type cells. During the latent period the infected cells were very sensitive to changes in growth conditions and in particular, dilution. Pre-conditioning of the bacterial cells by allowing them to stand for 24 h after shaking for 3 days was an important aspect of the stationary phase phage growth system. Cells which had been allowed to stand retained the ability to be infected and to support phage growth for at least 16 days. Shaking cultures gradually lost the ability to support phage growth but the phage could persist in the host cell for 10 days until removal from shaking when the lytic cycle could proceed after allowing the cultures to stand. In comparison the latent period and burst size in Achromobacter w.t. stationary phase cells were reduced to less than 2 h and less than 200 respectively. Stationary phase cultures differed physiologically and morphologically depending on the aeration conditions. In comparison with non-aerated standing cultures, vigorously aerated cultures showed a decrease in viability, RNA synthesis, membrane transport, intracellular ATP levels, UV resistance and heat resistance but had markedly higher protein synthesis levels. Aerated cells were small non-motile rods which did not support phage growth. They developed into large motile rods under conditions of limited aeration and were able to propagate phage. It was proposed that changes in the host control mechanisms for macromolecular synthesis may be instrumental in either blocking or permitting phage development. A spontaneous mutant of Achromobacter strain 14 (14x) which liberated phage and was resistant to superinfection was isolated. The phage-host relationship was unstable and similar to the phage carrier state. The liberated phage were able to grow in exponential strain 14 cells. It was proposed that strain 14 was a defective lysogen and that an immunity phase shift model may account for the differential phage growth in exponential and stationary phase cells. Host transcriptional control appears to be implicated in control of phage development in exponential and stationary phase cells. Achromobacter Lp only supported phage in exponential phase but a rifampicin resistant mutant of this strain was able to propagate phage in stationary phase. In vitro RNA synthesis assays showed that the rifampicin resistance was caused by an alteration in the RNA polymerase. Preliminary experiments to determine intracellular phage macromolecular synthesis were carried out using exponential Achromobacter w.t. cells which had been irradiated with UV prior to infection. In irradiated cells, infection with phage resulted in stimulation of DNA synthesis but no stimulation of protein synthesis. Phage production was drastically reduced in cells which had been treated with very low UV doses. It was proposed that α3a development may rely heavily on host cell functions which are destroyed by UV. Achromobacter mutants with defective leucine transport systems were isolated. Mutants which lost the leucine uptake system completely were totally resistant to phage infection and were unable to adsorb phage α3a. This is the first report to implicate an amino-acid transport system in phage adsorption.
- Full Text:
- Date Issued: 1980
- Authors: Robb, Susan Mary
- Date: 1980
- Subjects: Bacteriophages , Strains and stresses
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4125 , http://hdl.handle.net/10962/d1014131
- Description: Achromobacter w.t. and strain 14 both support phage α3a growth in stationary phase, but unlike the w.t. strain, exponential phase cultures of strain 14 block phage development. A standard method was developed for determining phage growth in stationary phase cultures. Lyophilised cells were used to eliminate variations due to the unstable phenotype of Achromobacter strain 14 cells. Phage α3a growth in stationary phase was characterized by a long and variable latent period of 6 to 9 h and an increased burst size of 709 p.f.u. per cell as compared with 153 p.f.u. per cell in exponential wild type cells. During the latent period the infected cells were very sensitive to changes in growth conditions and in particular, dilution. Pre-conditioning of the bacterial cells by allowing them to stand for 24 h after shaking for 3 days was an important aspect of the stationary phase phage growth system. Cells which had been allowed to stand retained the ability to be infected and to support phage growth for at least 16 days. Shaking cultures gradually lost the ability to support phage growth but the phage could persist in the host cell for 10 days until removal from shaking when the lytic cycle could proceed after allowing the cultures to stand. In comparison the latent period and burst size in Achromobacter w.t. stationary phase cells were reduced to less than 2 h and less than 200 respectively. Stationary phase cultures differed physiologically and morphologically depending on the aeration conditions. In comparison with non-aerated standing cultures, vigorously aerated cultures showed a decrease in viability, RNA synthesis, membrane transport, intracellular ATP levels, UV resistance and heat resistance but had markedly higher protein synthesis levels. Aerated cells were small non-motile rods which did not support phage growth. They developed into large motile rods under conditions of limited aeration and were able to propagate phage. It was proposed that changes in the host control mechanisms for macromolecular synthesis may be instrumental in either blocking or permitting phage development. A spontaneous mutant of Achromobacter strain 14 (14x) which liberated phage and was resistant to superinfection was isolated. The phage-host relationship was unstable and similar to the phage carrier state. The liberated phage were able to grow in exponential strain 14 cells. It was proposed that strain 14 was a defective lysogen and that an immunity phase shift model may account for the differential phage growth in exponential and stationary phase cells. Host transcriptional control appears to be implicated in control of phage development in exponential and stationary phase cells. Achromobacter Lp only supported phage in exponential phase but a rifampicin resistant mutant of this strain was able to propagate phage in stationary phase. In vitro RNA synthesis assays showed that the rifampicin resistance was caused by an alteration in the RNA polymerase. Preliminary experiments to determine intracellular phage macromolecular synthesis were carried out using exponential Achromobacter w.t. cells which had been irradiated with UV prior to infection. In irradiated cells, infection with phage resulted in stimulation of DNA synthesis but no stimulation of protein synthesis. Phage production was drastically reduced in cells which had been treated with very low UV doses. It was proposed that α3a development may rely heavily on host cell functions which are destroyed by UV. Achromobacter mutants with defective leucine transport systems were isolated. Mutants which lost the leucine uptake system completely were totally resistant to phage infection and were unable to adsorb phage α3a. This is the first report to implicate an amino-acid transport system in phage adsorption.
- Full Text:
- Date Issued: 1980
Baudelaire, nature and the artist in society
- Authors: Howell, Jane
- Date: 1980
- Subjects: Baudelaire, Charles, 1821-1867
- Language: English
- Type: Thesis , Masters , MFA
- Identifier: vital:2466 , http://hdl.handle.net/10962/d1009432
- Description: From Conclusion: The Artist can regard Baudelaire as a touchstone, as so many of his ideals and maxims are the ideals and maxims of the Artist himself. He teaches us many invaluable secrets of the universe and his lucid rendering of their explanations give us a clear insight into its mystery. He believed that Art was the ‘brainchild’ of Nature’s inspiration and that through its means and ways Natures mysteries will be revealed to us. He fought against all that the modern-day Artist is stiII fighting against. He rebelled against society1s false reasoning and its false morals. He became ‘self-exiled’ so that he could retain his individuality and reasoning. Like the Artist, his most valuable quality was his spontaneity and inspiration, given to him when his spirit moved him. His poems stand complete in themselves and yet all have a mysterious quality binding them. Likewise our paintings must also stand complete, they must be an end in themselves, each with its own singular message and yet a unity must prevaiI throughout. We must strive for that eternal quality that is so obvious in Baudelaire1s work. He can be read today at the distance of a century as if he had written for the present generation, with a knowledge of its problems and interests. His appeal is still vital because he was not fettered by the fashionable opinions and evanescent whims of his own age, and he made no concessions to the spirit of his own time in order to gain popularity.
- Full Text:
- Date Issued: 1980
- Authors: Howell, Jane
- Date: 1980
- Subjects: Baudelaire, Charles, 1821-1867
- Language: English
- Type: Thesis , Masters , MFA
- Identifier: vital:2466 , http://hdl.handle.net/10962/d1009432
- Description: From Conclusion: The Artist can regard Baudelaire as a touchstone, as so many of his ideals and maxims are the ideals and maxims of the Artist himself. He teaches us many invaluable secrets of the universe and his lucid rendering of their explanations give us a clear insight into its mystery. He believed that Art was the ‘brainchild’ of Nature’s inspiration and that through its means and ways Natures mysteries will be revealed to us. He fought against all that the modern-day Artist is stiII fighting against. He rebelled against society1s false reasoning and its false morals. He became ‘self-exiled’ so that he could retain his individuality and reasoning. Like the Artist, his most valuable quality was his spontaneity and inspiration, given to him when his spirit moved him. His poems stand complete in themselves and yet all have a mysterious quality binding them. Likewise our paintings must also stand complete, they must be an end in themselves, each with its own singular message and yet a unity must prevaiI throughout. We must strive for that eternal quality that is so obvious in Baudelaire1s work. He can be read today at the distance of a century as if he had written for the present generation, with a knowledge of its problems and interests. His appeal is still vital because he was not fettered by the fashionable opinions and evanescent whims of his own age, and he made no concessions to the spirit of his own time in order to gain popularity.
- Full Text:
- Date Issued: 1980
Black urban employment and Coloured labour preference
- Bekker, S B, Coetzee, Johannes Hendrik
- Authors: Bekker, S B , Coetzee, Johannes Hendrik
- Date: 1980
- Subjects: Black people -- Employment -- South Africa -- Cape Town Colored people (South Africa) -- Employment -- South Africa -- Cape Town Labor supply -- South Africa -- Cape Town Health services administration -- South Africa
- Language: English
- Type: Book , Text
- Identifier: http://hdl.handle.net/10962/2216 , vital:20266 , ISBN 0868100420
- Description: State control over the labour market usually results in the creation of different categories of labour, each having partial and unequal access to that market. In the Third World, typically, justification for control arises from an over-supply of unskilled rural labour and a small demand for such labour in the wage economy. To reduce massive urban unemployment, the state attempts to control the process of rural-urban migration by, inter alia, manipulating the labour market. ^ In South Africa, the civilised labour policy of the Pact government and the highly sophisticated system of black influx control introduced after the Second World War are two examples of such state control. In these cases, racial categories of labour, having differential access to the labour market, were created. The policy of Coloured Labour Preference is another South African example which is of particular interest since it is applied solely to one region of the Republic. Since 1962, when it was administratively coordinated for the first time, this policy has been applied in the Western Cape, a region comprising the 68 magisterial districts situated south-west of Port Elizabeth, Kimberley and the Orange River. In this region, black work-seekers' access to the labour market is severely curtailed. A series of regulations, particular to the Western Cape, are applied with the object of restricting the number of blacks resident in the region; denying blacks permanent rights of sojourn in the region; restricting the scope of employment for blacks in the region; and favouring coloured above black work-seekers throughout the region. In short, the policy aims to replace black by coloured labour and thereby aims to reduce to a minimum the number of blacks in the region. , Digitised by Rhodes University Library on behalf of the Institute of Social and Economic Research (ISER)
- Full Text:
- Date Issued: 1980
- Authors: Bekker, S B , Coetzee, Johannes Hendrik
- Date: 1980
- Subjects: Black people -- Employment -- South Africa -- Cape Town Colored people (South Africa) -- Employment -- South Africa -- Cape Town Labor supply -- South Africa -- Cape Town Health services administration -- South Africa
- Language: English
- Type: Book , Text
- Identifier: http://hdl.handle.net/10962/2216 , vital:20266 , ISBN 0868100420
- Description: State control over the labour market usually results in the creation of different categories of labour, each having partial and unequal access to that market. In the Third World, typically, justification for control arises from an over-supply of unskilled rural labour and a small demand for such labour in the wage economy. To reduce massive urban unemployment, the state attempts to control the process of rural-urban migration by, inter alia, manipulating the labour market. ^ In South Africa, the civilised labour policy of the Pact government and the highly sophisticated system of black influx control introduced after the Second World War are two examples of such state control. In these cases, racial categories of labour, having differential access to the labour market, were created. The policy of Coloured Labour Preference is another South African example which is of particular interest since it is applied solely to one region of the Republic. Since 1962, when it was administratively coordinated for the first time, this policy has been applied in the Western Cape, a region comprising the 68 magisterial districts situated south-west of Port Elizabeth, Kimberley and the Orange River. In this region, black work-seekers' access to the labour market is severely curtailed. A series of regulations, particular to the Western Cape, are applied with the object of restricting the number of blacks resident in the region; denying blacks permanent rights of sojourn in the region; restricting the scope of employment for blacks in the region; and favouring coloured above black work-seekers throughout the region. In short, the policy aims to replace black by coloured labour and thereby aims to reduce to a minimum the number of blacks in the region. , Digitised by Rhodes University Library on behalf of the Institute of Social and Economic Research (ISER)
- Full Text:
- Date Issued: 1980
Change is not made without inconvenience
- Authors: Bozzoli, G R
- Date: 1980
- Subjects: Academic Freedom -- South Africa Social change -- South Africa Education and state -- South Africa Education -- South Africa -- Aims and objectives Education -- Standards -- South Africa Black people -- Education -- South Africa Universities and colleges -- South Africa School integration -- South Africa Discrimination in education -- South Africa
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/259 , vital:19942
- Description: "Change is not made without inconvenience, even from worse to better". I propose to examine a few of these “inconveniences", some of which may turn out not to be inconvenient at all, while others may mean a complete revision of life style, or abandonment of a cherished ideal. In either case, a most vital issue surrounding change is a quantity well known to scientists and engineers, austronauts and motorists, the quantity known as the rate of change, or alternatively, the acceleration or deceleration. Change comes fastest when great pressures or forces are exerted, either revolutionary forces which are aimed at causing events to move rapidly, or oppositely, when the forces of authority are exerted to prevent matters from developing at all. These latter cause a deceleration of the movement of events, but both conditions represent high rates of change with the concomitant dangers that flow from the existence of inertia in the system and the people. Inertia in the accelerating condition results in the movement passing beyond control. Inertia in the decelerating condition entrenches those who are opposed to change and blocks all the natural outlets through which internal pressure could be relieved. Communication demands as a prerequisite, education, so that the essential link in the control chain lies in the schools and universities, and particularly in the universities. If the feedback is to come into play, then the universities must be the places where people learn to process the information. Universities are also the places where real change could originate as history has shown, so that either way, their role is vital. Paradoxically, although universities have, on the face of it, changed vastly over the centuries, and particularly during this half century, yet they have, by and large, retained their democratic character more successfully than any other institution. As 1 see it therefore, the universities should be and could be, very deeply involved in societal change,
- Full Text:
- Date Issued: 1980
- Authors: Bozzoli, G R
- Date: 1980
- Subjects: Academic Freedom -- South Africa Social change -- South Africa Education and state -- South Africa Education -- South Africa -- Aims and objectives Education -- Standards -- South Africa Black people -- Education -- South Africa Universities and colleges -- South Africa School integration -- South Africa Discrimination in education -- South Africa
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/259 , vital:19942
- Description: "Change is not made without inconvenience, even from worse to better". I propose to examine a few of these “inconveniences", some of which may turn out not to be inconvenient at all, while others may mean a complete revision of life style, or abandonment of a cherished ideal. In either case, a most vital issue surrounding change is a quantity well known to scientists and engineers, austronauts and motorists, the quantity known as the rate of change, or alternatively, the acceleration or deceleration. Change comes fastest when great pressures or forces are exerted, either revolutionary forces which are aimed at causing events to move rapidly, or oppositely, when the forces of authority are exerted to prevent matters from developing at all. These latter cause a deceleration of the movement of events, but both conditions represent high rates of change with the concomitant dangers that flow from the existence of inertia in the system and the people. Inertia in the accelerating condition results in the movement passing beyond control. Inertia in the decelerating condition entrenches those who are opposed to change and blocks all the natural outlets through which internal pressure could be relieved. Communication demands as a prerequisite, education, so that the essential link in the control chain lies in the schools and universities, and particularly in the universities. If the feedback is to come into play, then the universities must be the places where people learn to process the information. Universities are also the places where real change could originate as history has shown, so that either way, their role is vital. Paradoxically, although universities have, on the face of it, changed vastly over the centuries, and particularly during this half century, yet they have, by and large, retained their democratic character more successfully than any other institution. As 1 see it therefore, the universities should be and could be, very deeply involved in societal change,
- Full Text:
- Date Issued: 1980
Characterization and mode of action of a bacteriocin produced by a Bacteroides Fragilis strain
- Mossie, Godwin Mxolisi Kevin
- Authors: Mossie, Godwin Mxolisi Kevin
- Date: 1980
- Subjects: Bacteroides , Anaerobic bacteria , Trypsin , Dinitrophenol , Proteins -- Synthesis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4124 , http://hdl.handle.net/10962/d1013543
- Description: Bacteroides fragilis strain Bf-1 produces an extracellular bacteriocin at the beginning of the stationary growth phase. Production is not inducible by either ultraviolet light or mitomycin C. The low molecular weight bacteriocin (MW estimates of 13 500 and 18 800 obtained from Sephadex G-100 chromatography and SDS-PAGE electrophoresis respecively) is stable between pH 7 - 9 and is inactivated on incubation with trypsin and pronase. An unusual feature of the Bf-1 bacteriocin is its apparent biphasic temperature stability: while the majority of the activity (97%) is destroyed by heating at 60ºC (t [subscript] 1/2 = 2.5 min at 60ºC), a small proportion (3%) is stable even after autoclaving at 121ºC for 15 min. The killing of sensitive cells occurs in 2 stages and the killing action is reversed by incubation with trypsin. The transition from stage I to stage II is dependent on the temperature of incubation and the growth state of sensitive cells. 2,4-Dinitrophenol prevents this transition. The Bf-1 bacteriocin has an unusual mode of action. It specifically inhibits RNA synthesis whilst having no effect on protein or DNA synthesis. No effect on intracellular ATP levels were observed. The heat-stable (3%) fraction had a similar biochemical effect. In vitro studies involving RNA polymerase indicated that the bacteriocin and the antibiotic rifampicin have similar effects on RNA synthesis. The bacteriocinogenic strain (Bf-1) is insensitive to its own bacteriocin both in vivo and in vitro, although this immunity is overcome in vitro by the addition of higher concentrations of the Bf-1 bacteriocin. The bacteriocinogenic strain (Bf-1) harbors a cryptic plasmid (or plasmids) which on a neutral sucrose gradient, sediments faster than the Col E1 marker plasmid DNA. Attempts to cure this strain of its bacteriocinogenic phenotype were unsuccessful.
- Full Text:
- Date Issued: 1980
- Authors: Mossie, Godwin Mxolisi Kevin
- Date: 1980
- Subjects: Bacteroides , Anaerobic bacteria , Trypsin , Dinitrophenol , Proteins -- Synthesis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4124 , http://hdl.handle.net/10962/d1013543
- Description: Bacteroides fragilis strain Bf-1 produces an extracellular bacteriocin at the beginning of the stationary growth phase. Production is not inducible by either ultraviolet light or mitomycin C. The low molecular weight bacteriocin (MW estimates of 13 500 and 18 800 obtained from Sephadex G-100 chromatography and SDS-PAGE electrophoresis respecively) is stable between pH 7 - 9 and is inactivated on incubation with trypsin and pronase. An unusual feature of the Bf-1 bacteriocin is its apparent biphasic temperature stability: while the majority of the activity (97%) is destroyed by heating at 60ºC (t [subscript] 1/2 = 2.5 min at 60ºC), a small proportion (3%) is stable even after autoclaving at 121ºC for 15 min. The killing of sensitive cells occurs in 2 stages and the killing action is reversed by incubation with trypsin. The transition from stage I to stage II is dependent on the temperature of incubation and the growth state of sensitive cells. 2,4-Dinitrophenol prevents this transition. The Bf-1 bacteriocin has an unusual mode of action. It specifically inhibits RNA synthesis whilst having no effect on protein or DNA synthesis. No effect on intracellular ATP levels were observed. The heat-stable (3%) fraction had a similar biochemical effect. In vitro studies involving RNA polymerase indicated that the bacteriocin and the antibiotic rifampicin have similar effects on RNA synthesis. The bacteriocinogenic strain (Bf-1) is insensitive to its own bacteriocin both in vivo and in vitro, although this immunity is overcome in vitro by the addition of higher concentrations of the Bf-1 bacteriocin. The bacteriocinogenic strain (Bf-1) harbors a cryptic plasmid (or plasmids) which on a neutral sucrose gradient, sediments faster than the Col E1 marker plasmid DNA. Attempts to cure this strain of its bacteriocinogenic phenotype were unsuccessful.
- Full Text:
- Date Issued: 1980
Christian joy
- Authors: Williams, Denis Ivor
- Date: 1980
- Subjects: Joy -- Religious aspects -- Christianity Christian life
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1212 , http://hdl.handle.net/10962/d1001541
- Description: Christian joy is identified by linguistic and symbolic, experiential and psychological studies, and by a study of its opposite, sorrow. The final and most comprehensive approach is Biblical and theological. Here, through Judaism and Christianity, the genesis and fulfilment of Christian joy is examined, in life and the gifts of God, in hope, and in union with God. It is defined as "a gift of God's Holy Spirit as man becomes one with Christ in love." Five hypotheses are evaluated and confirmed: - 1. God is perfect joy, 2. God is the source and end of all Christian joy, 3. Jesus Christ is both the most joyful and the most sorrowful of men, 4. The Christian participates in the joy and sorrow of Jesus Christ, 5. Christian joy is eschatological in nature. The need is stressed for a fuller understanding and expression of Christian joy, which is seen as the complement of Christian love, and as a distinguishing characteristic of Christians, because of its primary intentional and ultimate satisfactory nature
- Full Text:
- Date Issued: 1980
- Authors: Williams, Denis Ivor
- Date: 1980
- Subjects: Joy -- Religious aspects -- Christianity Christian life
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1212 , http://hdl.handle.net/10962/d1001541
- Description: Christian joy is identified by linguistic and symbolic, experiential and psychological studies, and by a study of its opposite, sorrow. The final and most comprehensive approach is Biblical and theological. Here, through Judaism and Christianity, the genesis and fulfilment of Christian joy is examined, in life and the gifts of God, in hope, and in union with God. It is defined as "a gift of God's Holy Spirit as man becomes one with Christ in love." Five hypotheses are evaluated and confirmed: - 1. God is perfect joy, 2. God is the source and end of all Christian joy, 3. Jesus Christ is both the most joyful and the most sorrowful of men, 4. The Christian participates in the joy and sorrow of Jesus Christ, 5. Christian joy is eschatological in nature. The need is stressed for a fuller understanding and expression of Christian joy, which is seen as the complement of Christian love, and as a distinguishing characteristic of Christians, because of its primary intentional and ultimate satisfactory nature
- Full Text:
- Date Issued: 1980
Development of a high pressure liquid chromatographic method for the simultaneous analysis of sulphamethoxazole and trimethoprim and its application to biological fluids and dissolution rate studies on solid oral dosage forms
- Authors: Gochin, Rosa
- Date: 1980
- Subjects: High performance liquid chromatography , Body fluids -- Analysis , Drugs -- Dosage forms
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3735 , http://hdl.handle.net/10962/d1001524
- Description: Co-trimoxazole, a combination of a 5-to-l ratio of Sulphamethoxazole (SMZ) and Trimethoprim (TMP) , is a highly effective, broad-spectrum antibacterial agent. Since its introduction in 1968, it has been extensively used in infections of the respiratory and urinary tracts. Co-trimoxazole was developed by the systematic investigation of a series of compounds whose mechanism of action was already known. As early as 1950 synergy between sulphonamides and 2,4-diaminopyrimidines was reported. This was to be expected as both groups of drugs exert their antibacterial activity by interfering with the same biochemical pathway in bacteria. TMP was chosen from among many 2,4-diaminopyrimidines tested because of its good antibacterial activity and low toxicity. SMZ was chosen from the sulphonamides available for combination with TMP because of similarity of their biological half-lives. The widespread use of the combination coupled with the fact that monitoring of the levels of all drugs in the body is becoming increasingly important has stimulated research into rapid and efficient methods for the analysis of TMP and SMZ in biological fluids. Another consequence of the immense popularity of the combination is the appearance on the market of several generic preparations of Co-trimoxazole. It is now generally recognized that drug products from different manufacturers which are chemically equivalent may not be therapeutically equivalent. This is due to the fact that the absorption rate and/or bioavailability (extent of absorption) of a poorly soluble drug may be markedly affected by its release rate from the product and by its subsequent dissolution rate in gastrointestinal fluids. Hence bioequivalence of these various products should be established
- Full Text:
- Date Issued: 1980
- Authors: Gochin, Rosa
- Date: 1980
- Subjects: High performance liquid chromatography , Body fluids -- Analysis , Drugs -- Dosage forms
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3735 , http://hdl.handle.net/10962/d1001524
- Description: Co-trimoxazole, a combination of a 5-to-l ratio of Sulphamethoxazole (SMZ) and Trimethoprim (TMP) , is a highly effective, broad-spectrum antibacterial agent. Since its introduction in 1968, it has been extensively used in infections of the respiratory and urinary tracts. Co-trimoxazole was developed by the systematic investigation of a series of compounds whose mechanism of action was already known. As early as 1950 synergy between sulphonamides and 2,4-diaminopyrimidines was reported. This was to be expected as both groups of drugs exert their antibacterial activity by interfering with the same biochemical pathway in bacteria. TMP was chosen from among many 2,4-diaminopyrimidines tested because of its good antibacterial activity and low toxicity. SMZ was chosen from the sulphonamides available for combination with TMP because of similarity of their biological half-lives. The widespread use of the combination coupled with the fact that monitoring of the levels of all drugs in the body is becoming increasingly important has stimulated research into rapid and efficient methods for the analysis of TMP and SMZ in biological fluids. Another consequence of the immense popularity of the combination is the appearance on the market of several generic preparations of Co-trimoxazole. It is now generally recognized that drug products from different manufacturers which are chemically equivalent may not be therapeutically equivalent. This is due to the fact that the absorption rate and/or bioavailability (extent of absorption) of a poorly soluble drug may be markedly affected by its release rate from the product and by its subsequent dissolution rate in gastrointestinal fluids. Hence bioequivalence of these various products should be established
- Full Text:
- Date Issued: 1980
Directory of welfare, religious and service organisations, social and sporting clubs and other societies and councils in Grahamstown
- Authors: Henderson, Thelma M
- Date: 1980
- Subjects: Associations, institutions, etc. -- South Africa -- Grahamstown -- Directories Social service -- South Africa -- Grahamstown -- Directories Church buildings -- South Africa -- Grahamstown Community education -- South Africa -- Eastern Cape Sports -- South Africa -- South Africa -- Grahamstown Social service -- South Africa -- Grahamstown Charities -- South Africa -- Grahamstown Societies -- South Africa -- Grahamstown Grahamstown -- South Africa -- Social conditions
- Language: English
- Type: Book , Text
- Identifier: http://hdl.handle.net/10962/2790 , vital:20326 , ISBN 0868100293
- Description: This initial attempt to compile a register of the various and numerous organisations in Grahamstown has been undertaken by the Albany branch of the National Council of Women. It has been my pleasure as president to research and edit this work. 1 must stress that this is neither complete nor all-embracing, but it was decided to publish now in order that the information which has presently been assembled be reasonably up to date. Where details concerning a particular society or group are scant a space has been left for interested readers to insert independently any additional information they are able to secure. Should the general public wish this to be an annual publication the NCW will be prepared to undertake the work. With this in mind I shall be most grateful to receive the details of any groups which have been inadvertently omitted as well as changed information regarding those groups which have been included. I hope that Grahamstonians will enjoy perusing this list of so many different kinds of involvement. It would be interesting to know whether we hold some kind of record in this regard. We are a small community, but most interests are represented and there is something for practically everyone. It is hoped that newcomers to the city will make use of this directory to make contact with the sporting club, service group or social association that best suits their interests and inclinations. , Digitised by Rhodes University Library on behalf of the Institute of Social and Economic Research (ISER)
- Full Text:
- Date Issued: 1980
- Authors: Henderson, Thelma M
- Date: 1980
- Subjects: Associations, institutions, etc. -- South Africa -- Grahamstown -- Directories Social service -- South Africa -- Grahamstown -- Directories Church buildings -- South Africa -- Grahamstown Community education -- South Africa -- Eastern Cape Sports -- South Africa -- South Africa -- Grahamstown Social service -- South Africa -- Grahamstown Charities -- South Africa -- Grahamstown Societies -- South Africa -- Grahamstown Grahamstown -- South Africa -- Social conditions
- Language: English
- Type: Book , Text
- Identifier: http://hdl.handle.net/10962/2790 , vital:20326 , ISBN 0868100293
- Description: This initial attempt to compile a register of the various and numerous organisations in Grahamstown has been undertaken by the Albany branch of the National Council of Women. It has been my pleasure as president to research and edit this work. 1 must stress that this is neither complete nor all-embracing, but it was decided to publish now in order that the information which has presently been assembled be reasonably up to date. Where details concerning a particular society or group are scant a space has been left for interested readers to insert independently any additional information they are able to secure. Should the general public wish this to be an annual publication the NCW will be prepared to undertake the work. With this in mind I shall be most grateful to receive the details of any groups which have been inadvertently omitted as well as changed information regarding those groups which have been included. I hope that Grahamstonians will enjoy perusing this list of so many different kinds of involvement. It would be interesting to know whether we hold some kind of record in this regard. We are a small community, but most interests are represented and there is something for practically everyone. It is hoped that newcomers to the city will make use of this directory to make contact with the sporting club, service group or social association that best suits their interests and inclinations. , Digitised by Rhodes University Library on behalf of the Institute of Social and Economic Research (ISER)
- Full Text:
- Date Issued: 1980
Event in honour of Doctor Louis Dubb
- Authors: Henderson, Derek Scott
- Date: 1980
- Language: English
- Type: Text
- Identifier: vital:7421 , http://hdl.handle.net/10962/d1018298
- Full Text:
- Date Issued: 1980
- Authors: Henderson, Derek Scott
- Date: 1980
- Language: English
- Type: Text
- Identifier: vital:7421 , http://hdl.handle.net/10962/d1018298
- Full Text:
- Date Issued: 1980
Event in honour of Doctor Sir Michael Edwardes
- Authors: Henderson, Derek Scott
- Date: 1980
- Language: English
- Type: Text
- Identifier: vital:7420 , http://hdl.handle.net/10962/d1018297
- Full Text:
- Date Issued: 1980
- Authors: Henderson, Derek Scott
- Date: 1980
- Language: English
- Type: Text
- Identifier: vital:7420 , http://hdl.handle.net/10962/d1018297
- Full Text:
- Date Issued: 1980
Event in honour of Reverend Bill Burnett
- Authors: Henderson, Derek Scott
- Date: 1980
- Language: English
- Type: Text
- Identifier: vital:7419 , http://hdl.handle.net/10962/d1018296
- Full Text:
- Date Issued: 1980
- Authors: Henderson, Derek Scott
- Date: 1980
- Language: English
- Type: Text
- Identifier: vital:7419 , http://hdl.handle.net/10962/d1018296
- Full Text:
- Date Issued: 1980
First H F Doppler soundings of the ionosphere at SANAE
- Authors: De Kock, Errol James
- Date: 1980
- Subjects: Ionosphere
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5500 , http://hdl.handle.net/10962/d1006869 , Ionosphere
- Full Text:
- Date Issued: 1980
- Authors: De Kock, Errol James
- Date: 1980
- Subjects: Ionosphere
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5500 , http://hdl.handle.net/10962/d1006869 , Ionosphere
- Full Text:
- Date Issued: 1980
FOSATU Notice day
- FOSATU
- Authors: FOSATU
- Date: Apr 1980
- Subjects: FOSATU
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/118508 , vital:34641
- Description: This booklet gives the minimum period of notice required before either the employer or his employee may terminate his contract of employment as laid down in the various wage regulating measures ie. Wage Determinations, Industrial Council Agreements and Wage Orders. The booklet must be used in conjunction with the: "The complete guide to wage regulation measures" by H. Cheadle and L. Silver to determine the exact area and industrial scope to which each particular measure applies. The notice period given in the booklet is of course the minimum required and it can be varied by employers in determining individual contracts of employment.
- Full Text:
- Date Issued: Apr 1980
- Authors: FOSATU
- Date: Apr 1980
- Subjects: FOSATU
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/118508 , vital:34641
- Description: This booklet gives the minimum period of notice required before either the employer or his employee may terminate his contract of employment as laid down in the various wage regulating measures ie. Wage Determinations, Industrial Council Agreements and Wage Orders. The booklet must be used in conjunction with the: "The complete guide to wage regulation measures" by H. Cheadle and L. Silver to determine the exact area and industrial scope to which each particular measure applies. The notice period given in the booklet is of course the minimum required and it can be varied by employers in determining individual contracts of employment.
- Full Text:
- Date Issued: Apr 1980
Genetic studies and physiological responses to ultraviolet radiation in the Bacteroides fragilis group
- Authors: Jones, David Todman
- Date: 1980
- Subjects: Bacteroides Ultraviolet radiation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4072 , http://hdl.handle.net/10962/d1007047
- Description: The contents of this thesis have been divided into 2 parts . The first part deals with genetic studies carried out on 36 strains belonging to the Bacteroides fragilis group. A number of mutants were isolated from several of the strains. A notable feature of the methods used was the low yield of mutants obtained and the marked sensitivity of these organisms to the mutagenic agents. Variations in colonial morphology was found to be a common feature amongst these organisms. In a few strains this phenomenon was clearly visible, in the remainder it was much weaker, and often could only be seen with the aid of a microscope . Colonial variation was found to be due to the ability of a proporti on of the cells to pruduce capsules or slime layers. The variants were found to segregate at high frequency and different growth conditions were found to have little effect on the segregation frequency or capsule formation . A number of phages specific for B. fragilis and B. t hetaiotaomicron were isol ated. All these phages were virulent and attempts to induce lysogenic phages were unsuccesful . The use of these phages in attempts to obtain transduction proved unsuccessful. A phage carrier state was found to occur in the majority of the phage-host cell systems, which seemed to be due to the presence of phage-resistant encapsulated cells in the population. Bacteriocins were produced by about half the strains, these inhibited the growth of a high proportion of the 36 strains tested. The bacteriocins were released into the growth media at the end of the growth period in the 2 bacteriocins tested. A link between the mode of action of one bacteriocin and rifampicin-resistance was investigated. All the bacter iocins tested were found to be inactive against some rifampicin-resistant mutants of a susceptible strain, suggesting a common mode of action. The presence of capsules in some cells appeared to confer bacteriocin-resistance on these variants. The second part of the thesis deals with a study of the physiological responses of a single strain of B.fragilis to ultraviolet radiation. This strain was found to be more sensitive to ultraviolet radiation under aerobic conditions. The amount of pyrimidine dimers formed after irradiation under anaerobic and aerobic conditions, was not found to differ significantly, indicating that the increase in sensitivity under aerobic conditions was not due to an increase in DNA damage. The use of repair inhibitors and the survival characteristics indicate that this difference was due to decreased repair capabilities under aerobic conditions. Liquid holding recovery in B.fragiZis was found to occur under aerobic conditions . This process was brought about by excision repair and appeared to be due to a decrease in repair efficiency under aerobic conditions. Under anaerobic conditions, where full repair capabilities were present, liquid holding recovery was inhibited. Both minimal medium recovery and fluence dependent filament formation were found to occur in irradiated B.fragiZis cells. The survival kinetics of a number of irradiated B.fragiZis phages were determined and a number of phage reactivation processes were investigated. Little or no host cell reactivation appeared to occur in the strains investigated, however, some ultraviolet reactivation and multiplicity reactivation was found to occur, but only under anaerobic conditions. Photoreactivation was found to be absent in this organism, but an excision repair system was present . The excision repair system was partially characterized and was found to resemble short patch excision repair in E.coli. Evidence was found which suggested that a second mode of repair which was sensitive to oxygen, also occurred in this strain. This repair system which appeared to be responsible for error-prone repair, and the systems which were responsible for ultraviolet reactivation and multiplicity reactivation, seemed to be dependent on a recombination function' which was inhibited by oxygen. The significance of this finding for future genetic studies was discussed.
- Full Text:
- Date Issued: 1980
- Authors: Jones, David Todman
- Date: 1980
- Subjects: Bacteroides Ultraviolet radiation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4072 , http://hdl.handle.net/10962/d1007047
- Description: The contents of this thesis have been divided into 2 parts . The first part deals with genetic studies carried out on 36 strains belonging to the Bacteroides fragilis group. A number of mutants were isolated from several of the strains. A notable feature of the methods used was the low yield of mutants obtained and the marked sensitivity of these organisms to the mutagenic agents. Variations in colonial morphology was found to be a common feature amongst these organisms. In a few strains this phenomenon was clearly visible, in the remainder it was much weaker, and often could only be seen with the aid of a microscope . Colonial variation was found to be due to the ability of a proporti on of the cells to pruduce capsules or slime layers. The variants were found to segregate at high frequency and different growth conditions were found to have little effect on the segregation frequency or capsule formation . A number of phages specific for B. fragilis and B. t hetaiotaomicron were isol ated. All these phages were virulent and attempts to induce lysogenic phages were unsuccesful . The use of these phages in attempts to obtain transduction proved unsuccessful. A phage carrier state was found to occur in the majority of the phage-host cell systems, which seemed to be due to the presence of phage-resistant encapsulated cells in the population. Bacteriocins were produced by about half the strains, these inhibited the growth of a high proportion of the 36 strains tested. The bacteriocins were released into the growth media at the end of the growth period in the 2 bacteriocins tested. A link between the mode of action of one bacteriocin and rifampicin-resistance was investigated. All the bacter iocins tested were found to be inactive against some rifampicin-resistant mutants of a susceptible strain, suggesting a common mode of action. The presence of capsules in some cells appeared to confer bacteriocin-resistance on these variants. The second part of the thesis deals with a study of the physiological responses of a single strain of B.fragilis to ultraviolet radiation. This strain was found to be more sensitive to ultraviolet radiation under aerobic conditions. The amount of pyrimidine dimers formed after irradiation under anaerobic and aerobic conditions, was not found to differ significantly, indicating that the increase in sensitivity under aerobic conditions was not due to an increase in DNA damage. The use of repair inhibitors and the survival characteristics indicate that this difference was due to decreased repair capabilities under aerobic conditions. Liquid holding recovery in B.fragiZis was found to occur under aerobic conditions . This process was brought about by excision repair and appeared to be due to a decrease in repair efficiency under aerobic conditions. Under anaerobic conditions, where full repair capabilities were present, liquid holding recovery was inhibited. Both minimal medium recovery and fluence dependent filament formation were found to occur in irradiated B.fragiZis cells. The survival kinetics of a number of irradiated B.fragiZis phages were determined and a number of phage reactivation processes were investigated. Little or no host cell reactivation appeared to occur in the strains investigated, however, some ultraviolet reactivation and multiplicity reactivation was found to occur, but only under anaerobic conditions. Photoreactivation was found to be absent in this organism, but an excision repair system was present . The excision repair system was partially characterized and was found to resemble short patch excision repair in E.coli. Evidence was found which suggested that a second mode of repair which was sensitive to oxygen, also occurred in this strain. This repair system which appeared to be responsible for error-prone repair, and the systems which were responsible for ultraviolet reactivation and multiplicity reactivation, seemed to be dependent on a recombination function' which was inhibited by oxygen. The significance of this finding for future genetic studies was discussed.
- Full Text:
- Date Issued: 1980