An investigation into the extended use of mobile phone technology in the cellular industry in Port Elizabeth
- Authors: Inman, Michael Christopher
- Date: 2004
- Subjects: Cell phone services industry -- South Africa -- Port Elizabeth , Cell phones , Cell phone systems
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10923 , http://hdl.handle.net/10948/247 , Cell phone services industry -- South Africa -- Port Elizabeth , Cell phones , Cell phone systems
- Description: While experiencing exponential growth worldwide, Information and Communication Technologies (ICTs) are key factors in business today. The ubiquitous nature of the mobile phone in South Africa and the use of wireless technologies facilitate business in areas where current fixed infrastructure is limited or non-existent, thereby aiding in the development of rural areas. This descriptive study determines what factors will extend the use of mobile phone technology in business. The literature indicates business advantage can be gained from incorporating technology into a strategic framework but in so doing must consider the complexities of the technology and its lifecycle. Currently, e-mail and Internet access would be the most likely applications of data use via Global System for Mobile Communications (GSM). As a technology-based study the methodology used e-mail to deliver a survey targeting staff from the three cellular network operators in Port Elizabeth. The mobile industry and Port Elizabeth were both considered good indicators of technology use and the progression of infrastructure throughout South Africa. The findings indicate that although most mobile phones have the features required by users, the current use of associated data applications is low. Email is the most appropriate application and expected to be significant in 2006. The technology is of strategic importance and can be applied to make idle time productive. Successful use depends on the removal of barriers. In the case of e-mail and Internet access; the most significant of these is the complexity of the initial set-up. Despite the complexity of technology evolution and the challenges of effective business application, substantial gains can be still be realised. Currently, technology is best deployed to meet specialised needs. The expected introduction of third generation technologies herald’s higher data transfer rates, providing the opportunity for more generalised application in business in the immediate future.
- Full Text:
- Date Issued: 2004
- Authors: Inman, Michael Christopher
- Date: 2004
- Subjects: Cell phone services industry -- South Africa -- Port Elizabeth , Cell phones , Cell phone systems
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10923 , http://hdl.handle.net/10948/247 , Cell phone services industry -- South Africa -- Port Elizabeth , Cell phones , Cell phone systems
- Description: While experiencing exponential growth worldwide, Information and Communication Technologies (ICTs) are key factors in business today. The ubiquitous nature of the mobile phone in South Africa and the use of wireless technologies facilitate business in areas where current fixed infrastructure is limited or non-existent, thereby aiding in the development of rural areas. This descriptive study determines what factors will extend the use of mobile phone technology in business. The literature indicates business advantage can be gained from incorporating technology into a strategic framework but in so doing must consider the complexities of the technology and its lifecycle. Currently, e-mail and Internet access would be the most likely applications of data use via Global System for Mobile Communications (GSM). As a technology-based study the methodology used e-mail to deliver a survey targeting staff from the three cellular network operators in Port Elizabeth. The mobile industry and Port Elizabeth were both considered good indicators of technology use and the progression of infrastructure throughout South Africa. The findings indicate that although most mobile phones have the features required by users, the current use of associated data applications is low. Email is the most appropriate application and expected to be significant in 2006. The technology is of strategic importance and can be applied to make idle time productive. Successful use depends on the removal of barriers. In the case of e-mail and Internet access; the most significant of these is the complexity of the initial set-up. Despite the complexity of technology evolution and the challenges of effective business application, substantial gains can be still be realised. Currently, technology is best deployed to meet specialised needs. The expected introduction of third generation technologies herald’s higher data transfer rates, providing the opportunity for more generalised application in business in the immediate future.
- Full Text:
- Date Issued: 2004
Isolation of antigenic peptides of Cowdria ruminantium and their encoding genes using a genome-derived phage display library
- Authors: Fehrsen, Jeanni
- Date: 2003
- Subjects: Bacteriophages -- Genetics Ruminants -- Diseases Heartwater
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3920 , http://hdl.handle.net/10962/d1003979
- Description: The development of new and effective vaccines and immunodiagnostic reagents requires the characterisation of antigenically relevant proteins and their interactions with the products of the immune system. Phage display technology was investigated as a means of elucidating some of the antigenic properties of the rickettsial parasite, Cowdria ruminantium (Cowdria). Randomly fragmented gene-derived libraries have been useful in elucidating viral and other epitopes, but only limited work has been done with entire genomes. A phage display library expressing a repertoire of Cowdria peptides was constructed. It was sufficiently large to represent the organism's genome, but lacked phages displaying peptides coded for by genes containing a Pvu II restriction enzyme site, including the one coding for the major antigenic protein 1 (MAP1). This was considered advantageous since MAP1 is immunodominant and has already been well characterised. Affinity selection with antibodies against Cowdria proteins other than MAP1 allowed several antibody-reactive peptides to be isolated. These selected sequences were placed in the context of the genome by screening a lambda bacteriophage library and by comparison with Cowdria DNA sequences. Apart from showing that antigenic mimics were present in the phage display library, six open reading frames encoding putative Cowdria proteins were identified. All had similarities to, or motifs in common with, membrane proteins and are thus likely to be exposed to the host's humoral immune system. Some of the proteins identified were larger than the antigens used to elicit the antibodies used for selection, probably as a result of the presence of cross-reactive epitopes. Despite limitations experienced when extending a fragmented-gene approach for epitope location to genomes, it was possible to identify an antigenic region on MAP1 by comparison with selected mimics. In addition, binding peptide sequences were identified with two monoclonal antibodies that had been raised against non-Cowdria antigens. An epitope on the VP7 protein of bluetongue virus was identified and peptides were found that reacted with a monoclonal antibody directed against malignant catarrhal fever virus. Thus, apart from being able to identify several potentially important Cowdria epitopes and genes, the fragmented-genome library holds promise as a universal reagent for identifying useful mimics.
- Full Text:
- Date Issued: 2003
- Authors: Fehrsen, Jeanni
- Date: 2003
- Subjects: Bacteriophages -- Genetics Ruminants -- Diseases Heartwater
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3920 , http://hdl.handle.net/10962/d1003979
- Description: The development of new and effective vaccines and immunodiagnostic reagents requires the characterisation of antigenically relevant proteins and their interactions with the products of the immune system. Phage display technology was investigated as a means of elucidating some of the antigenic properties of the rickettsial parasite, Cowdria ruminantium (Cowdria). Randomly fragmented gene-derived libraries have been useful in elucidating viral and other epitopes, but only limited work has been done with entire genomes. A phage display library expressing a repertoire of Cowdria peptides was constructed. It was sufficiently large to represent the organism's genome, but lacked phages displaying peptides coded for by genes containing a Pvu II restriction enzyme site, including the one coding for the major antigenic protein 1 (MAP1). This was considered advantageous since MAP1 is immunodominant and has already been well characterised. Affinity selection with antibodies against Cowdria proteins other than MAP1 allowed several antibody-reactive peptides to be isolated. These selected sequences were placed in the context of the genome by screening a lambda bacteriophage library and by comparison with Cowdria DNA sequences. Apart from showing that antigenic mimics were present in the phage display library, six open reading frames encoding putative Cowdria proteins were identified. All had similarities to, or motifs in common with, membrane proteins and are thus likely to be exposed to the host's humoral immune system. Some of the proteins identified were larger than the antigens used to elicit the antibodies used for selection, probably as a result of the presence of cross-reactive epitopes. Despite limitations experienced when extending a fragmented-gene approach for epitope location to genomes, it was possible to identify an antigenic region on MAP1 by comparison with selected mimics. In addition, binding peptide sequences were identified with two monoclonal antibodies that had been raised against non-Cowdria antigens. An epitope on the VP7 protein of bluetongue virus was identified and peptides were found that reacted with a monoclonal antibody directed against malignant catarrhal fever virus. Thus, apart from being able to identify several potentially important Cowdria epitopes and genes, the fragmented-genome library holds promise as a universal reagent for identifying useful mimics.
- Full Text:
- Date Issued: 2003
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