Salinity induced physiological responses in juvenile dusky kob, Argyrosomus japonicus (Sciaenidae)
- Authors: Bernatzeder, Andrea Katinka
- Date: 2009
- Subjects: Osmoregulation , Argyrosomus -- Effects of salt on -- South Africa , Sciaenidae -- Effects of salt on -- South Africa , Argyrosomus -- Physiology , Sciaenidae -- Physiology , Fishes -- Physiology , Biological control systems , Marine ecology -- South Africa , Fishery management -- South Africa , Mariculture -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5318 , http://hdl.handle.net/10962/d1005163 , Osmoregulation , Argyrosomus -- Effects of salt on -- South Africa , Sciaenidae -- Effects of salt on -- South Africa , Argyrosomus -- Physiology , Sciaenidae -- Physiology , Fishes -- Physiology , Biological control systems , Marine ecology -- South Africa , Fishery management -- South Africa , Mariculture -- South Africa
- Description: Fisheries management regulations for dusky kob Argyrosomus japonicus, an important commercial and recreational fisheries species, have failed and the stock is considered collapsed. It is important to take an ecosystems approach to management which includes understanding the effect of environmental factors on recruitment, abundance and distribution. The distribution of early juveniles (20-150 mm TL) in the wild appears to be restricted to the upper reaches of estuaries at salinities below 5 psu. Food availability could not explain the distribution of early juveniles. The aim of this study was to investigate the role of salinity on the distribution of early juvenile dusky kob (<150 mm TL) by examining physiological responses of juveniles exposed to a range of salinities under laboratory conditions. The hypothesis was that the physiological functioning of early juveniles would be optimised at the reduced salinities which they naturally occur at. The objectives of this study were to investigate the effect of salinity on: i) plasma osmolality; ii) growth, food conversion ratio and condition factor; and iii) gill histology with emphasis on chloride cell size and number. A preliminary study was undertaken to determine whether the use of 2-phenoxyethanol had an effect on plasma osmolality. Juveniles pithed prior to blood sampling were used as the control. Plasma osmolality was not affected by exposure or duration of exposure (2, 4, 6, 8, 10 min) to 2-phenoxyethanol. The ability of teleosts to regulate plasma osmolality over a wide range of salinities indicates their degree of ‘physiological euryhalinity’. Plasma osmolality of juveniles exposed to 5, 12 and 35 psu was measured every two weeks over a total of six weeks. Although juveniles were able to regulate plasma osmolality over the duration of the experiment, plasma osmolality at 5 and 12 psu was significantly lower than in fish maintained at 35 psu. Growth is used as an indicator of the relative energy used for osmoregulation at different salinities, as the energy used for osmoregulation becomes unavailable for growth. A nineweek growth experiment was conducted on juveniles exposed to 5, 12 and 35 psu. Juveniles grew and survived at all three salinities. However, growth of juveniles at 5 psu was significantly lower than at 12 and 35 psu. Other than a significantly greater weight gain at 35 psu relative to 12 psu, there was no significant difference in specific growth and length gain between juveniles at 12 and 35 psu. Food conversion ratio and condition factor at 12 and 35 psu were not significantly different, but food conversion ratio and condition factor at 5 psu was significantly greater and lower than at 35 psu respectively. In fish, gills are considered the major organ involved in osmoregulation. Within the gills, chloride cells are the predominant site of ion exchange which is driven by the Na⁺, K⁺- ATPase enzyme. Gill samples of juveniles exposed to 5, 12 and 35 psu for six weeks were examined histologically using light microscopy. Chloride cells of juveniles maintained at 5 psu were significantly more abundant than in juveniles at 12 and 35 psu. Chloride cells of juveniles at 5 psu were significantly larger than in juveniles kept at 12 psu, but not significantly different to those of juveniles kept at 35 psu. The ability of the juvenile fish to regulate plasma osmolality indicates that they are 'physiologically euryhaline', but the reduced growth and proliferation of chloride cells at 5 psu suggests that energy expenditure for osmoregulation is increased at hypoosmotic salinities. Salinity induced physiological responses could therefore not explain the natural distribution of early juvenile dusky kob and it is proposed that other environmental factors (e.g. temperature) are also important. It is also hypothesised that the high conductivity of an estuary in South Africa, to which our understanding is limited, may negate the effect of reduced salinity. Although freshwater input into estuaries is an important factor, further investigations to explain the distribution and abundance of early juveniles is required to make management recommendations. Dusky kob is also becoming an increasingly popular aquaculture species in South Africa. In this regard, early juvenile dusky kob can be grown at salinities as low as 12 psu without negatively affecting growth and production.
- Full Text:
- Date Issued: 2009
- Authors: Bernatzeder, Andrea Katinka
- Date: 2009
- Subjects: Osmoregulation , Argyrosomus -- Effects of salt on -- South Africa , Sciaenidae -- Effects of salt on -- South Africa , Argyrosomus -- Physiology , Sciaenidae -- Physiology , Fishes -- Physiology , Biological control systems , Marine ecology -- South Africa , Fishery management -- South Africa , Mariculture -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5318 , http://hdl.handle.net/10962/d1005163 , Osmoregulation , Argyrosomus -- Effects of salt on -- South Africa , Sciaenidae -- Effects of salt on -- South Africa , Argyrosomus -- Physiology , Sciaenidae -- Physiology , Fishes -- Physiology , Biological control systems , Marine ecology -- South Africa , Fishery management -- South Africa , Mariculture -- South Africa
- Description: Fisheries management regulations for dusky kob Argyrosomus japonicus, an important commercial and recreational fisheries species, have failed and the stock is considered collapsed. It is important to take an ecosystems approach to management which includes understanding the effect of environmental factors on recruitment, abundance and distribution. The distribution of early juveniles (20-150 mm TL) in the wild appears to be restricted to the upper reaches of estuaries at salinities below 5 psu. Food availability could not explain the distribution of early juveniles. The aim of this study was to investigate the role of salinity on the distribution of early juvenile dusky kob (<150 mm TL) by examining physiological responses of juveniles exposed to a range of salinities under laboratory conditions. The hypothesis was that the physiological functioning of early juveniles would be optimised at the reduced salinities which they naturally occur at. The objectives of this study were to investigate the effect of salinity on: i) plasma osmolality; ii) growth, food conversion ratio and condition factor; and iii) gill histology with emphasis on chloride cell size and number. A preliminary study was undertaken to determine whether the use of 2-phenoxyethanol had an effect on plasma osmolality. Juveniles pithed prior to blood sampling were used as the control. Plasma osmolality was not affected by exposure or duration of exposure (2, 4, 6, 8, 10 min) to 2-phenoxyethanol. The ability of teleosts to regulate plasma osmolality over a wide range of salinities indicates their degree of ‘physiological euryhalinity’. Plasma osmolality of juveniles exposed to 5, 12 and 35 psu was measured every two weeks over a total of six weeks. Although juveniles were able to regulate plasma osmolality over the duration of the experiment, plasma osmolality at 5 and 12 psu was significantly lower than in fish maintained at 35 psu. Growth is used as an indicator of the relative energy used for osmoregulation at different salinities, as the energy used for osmoregulation becomes unavailable for growth. A nineweek growth experiment was conducted on juveniles exposed to 5, 12 and 35 psu. Juveniles grew and survived at all three salinities. However, growth of juveniles at 5 psu was significantly lower than at 12 and 35 psu. Other than a significantly greater weight gain at 35 psu relative to 12 psu, there was no significant difference in specific growth and length gain between juveniles at 12 and 35 psu. Food conversion ratio and condition factor at 12 and 35 psu were not significantly different, but food conversion ratio and condition factor at 5 psu was significantly greater and lower than at 35 psu respectively. In fish, gills are considered the major organ involved in osmoregulation. Within the gills, chloride cells are the predominant site of ion exchange which is driven by the Na⁺, K⁺- ATPase enzyme. Gill samples of juveniles exposed to 5, 12 and 35 psu for six weeks were examined histologically using light microscopy. Chloride cells of juveniles maintained at 5 psu were significantly more abundant than in juveniles at 12 and 35 psu. Chloride cells of juveniles at 5 psu were significantly larger than in juveniles kept at 12 psu, but not significantly different to those of juveniles kept at 35 psu. The ability of the juvenile fish to regulate plasma osmolality indicates that they are 'physiologically euryhaline', but the reduced growth and proliferation of chloride cells at 5 psu suggests that energy expenditure for osmoregulation is increased at hypoosmotic salinities. Salinity induced physiological responses could therefore not explain the natural distribution of early juvenile dusky kob and it is proposed that other environmental factors (e.g. temperature) are also important. It is also hypothesised that the high conductivity of an estuary in South Africa, to which our understanding is limited, may negate the effect of reduced salinity. Although freshwater input into estuaries is an important factor, further investigations to explain the distribution and abundance of early juveniles is required to make management recommendations. Dusky kob is also becoming an increasingly popular aquaculture species in South Africa. In this regard, early juvenile dusky kob can be grown at salinities as low as 12 psu without negatively affecting growth and production.
- Full Text:
- Date Issued: 2009
Toward the development of a rearing protocol for juvenile dusky kob, Argyrosomus japonicus (Pisces: Sciaenidae)
- Authors: Collett, Paul David
- Date: 2008
- Subjects: Argyrosomus -- South Africa , Mariculture -- South Africa , Fishes -- Nutrition -- South Africa , Fishes -- Growth -- South Africa , Fish trade -- South Africa , Sciaenidae
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5325 , http://hdl.handle.net/10962/d1005171 , Argyrosomus -- South Africa , Mariculture -- South Africa , Fishes -- Nutrition -- South Africa , Fishes -- Growth -- South Africa , Fish trade -- South Africa , Sciaenidae
- Description: The South African mariculture industry is developing the rearing technology of indigenous fish species. Dusky kob (Argyrosomus japonicus) has high-quality flesh and a good market demand. Research is needed to determine the environmental requirements under which growth of dusky kob is optimised. This study assessed the effect of temperature, light intensity, feeding frequency and stocking density, respectively, on growth, feed conversion ratio (FCR) and survival of juvenile dusky kob within a weight range of 10-60 g fish⁻¹) in a series of four growth trials. The effect of temperature on growth and FCR was assessed at 14 temperatures from 17 to 28°C. The temperature for best growth was estimated to be 25.3 °C, while 21.4 °C was the temperature at which the best FCR was achieved. A growth trial testing the effect of light intensity on growth showed that light intensity did not affect growth in the range of 23 – 315 lx. Fish fed a restricted ration of 3.6% body weight per day (chapter 2) or 3.41% body weight per day (chapter 4) had a better FCR than fish fed to satiation. A trial to assess the effect of feeding frequency on growth and FCR showed that fish fed both two or three times daily grew better than those fed once daily. FCR was best in fish that were fed once or twice daily. Preliminary analysis of the results from a stocking density trial showed that stocking density in the range of 10 – 50 kg m⁻³ did not affect growth of juvenile dusky kob. It is recommended to culture dusky kob at a temperature of 25.3 - 21.4 °C at stocking densities up 50 kg m⁻³ to maximise growth. However, a study is needed to determine the environmental conditions needed to maximise profit under commercial conditions. The results allow the South African industry the opportunity to assess the culture potential of this species. In addition, the results will help develop protocols that can be used in other South African candidate aquaculture species such as silver kob, yellowtail, white-stumpnose and sole.
- Full Text:
- Date Issued: 2008
- Authors: Collett, Paul David
- Date: 2008
- Subjects: Argyrosomus -- South Africa , Mariculture -- South Africa , Fishes -- Nutrition -- South Africa , Fishes -- Growth -- South Africa , Fish trade -- South Africa , Sciaenidae
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5325 , http://hdl.handle.net/10962/d1005171 , Argyrosomus -- South Africa , Mariculture -- South Africa , Fishes -- Nutrition -- South Africa , Fishes -- Growth -- South Africa , Fish trade -- South Africa , Sciaenidae
- Description: The South African mariculture industry is developing the rearing technology of indigenous fish species. Dusky kob (Argyrosomus japonicus) has high-quality flesh and a good market demand. Research is needed to determine the environmental requirements under which growth of dusky kob is optimised. This study assessed the effect of temperature, light intensity, feeding frequency and stocking density, respectively, on growth, feed conversion ratio (FCR) and survival of juvenile dusky kob within a weight range of 10-60 g fish⁻¹) in a series of four growth trials. The effect of temperature on growth and FCR was assessed at 14 temperatures from 17 to 28°C. The temperature for best growth was estimated to be 25.3 °C, while 21.4 °C was the temperature at which the best FCR was achieved. A growth trial testing the effect of light intensity on growth showed that light intensity did not affect growth in the range of 23 – 315 lx. Fish fed a restricted ration of 3.6% body weight per day (chapter 2) or 3.41% body weight per day (chapter 4) had a better FCR than fish fed to satiation. A trial to assess the effect of feeding frequency on growth and FCR showed that fish fed both two or three times daily grew better than those fed once daily. FCR was best in fish that were fed once or twice daily. Preliminary analysis of the results from a stocking density trial showed that stocking density in the range of 10 – 50 kg m⁻³ did not affect growth of juvenile dusky kob. It is recommended to culture dusky kob at a temperature of 25.3 - 21.4 °C at stocking densities up 50 kg m⁻³ to maximise growth. However, a study is needed to determine the environmental conditions needed to maximise profit under commercial conditions. The results allow the South African industry the opportunity to assess the culture potential of this species. In addition, the results will help develop protocols that can be used in other South African candidate aquaculture species such as silver kob, yellowtail, white-stumpnose and sole.
- Full Text:
- Date Issued: 2008
The development of a geographic information system (GIS) as a mariculture sector planning tool in South Africa
- Authors: Shiran-Klotz, Imran
- Date: 2004
- Subjects: Geographic information systems -- South Africa , Fishery management -- Geographic information systems -- South Africa , Mariculture -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5337 , http://hdl.handle.net/10962/d1005870 , Geographic information systems -- South Africa , Fishery management -- Geographic information systems -- South Africa , Mariculture -- South Africa
- Description: The South African coastline has a high potential for mariculture development, particularly in land-based systems, due to excellent water quality, good infrastructure, and relatively cheap land in certain areas. Development of mariculture however, has been slow primarily due to the absence of a national sector development plan to coordinate contribution to development by government, industry and academia. Recent mariculture development plans however, supported by government commitment to stimulate coastal development, offers new opportunity for the sector's growth. These new sector development plans require a multi-disciplinary intensive information base with a strong regional and national spatial component. Geographic Information System (GIS) is a spatial analytical tool, which is capable of handling such large coastal databases and analysing them. Nevertheless, many mariculture GIS planning applications, often developed in isolation and with limited practical use for decision makers, highlighted the need to develop GIS in relation to the mariculture sector development plan. Hence, the aim of this study was to develop and evaluate a GIS for the national and regional mariculture sector planning process in South Africa, in order to identify potentially suitable areas along the South African coastline for mariculture operations. The GIS development in this study was based on the mariculture planning approach developed by PAP/RAe (1996), which recognizes the variations of scales in mariculture planning. Three case studies, representing the diversity of the South African sector plan, were selected to develop the GIS. They included: a national suitability analysis for all land-based culture systems, a specific national analysis on abalone ranching, and a detailed regional analysis of land-based culture and abalone ranching along the Namaqualand coastline. The GIS was developed separately for each case study based on a strategy consisting of six main phases, including: identification of project requirements, developing analytical framework, selection and location of data sources, organization and manipulation of data, analysing data and verifying and evaluation of the outputs. Biophysical, coastal use, and infrastructure criteria were collected, sorted and analysed to identify development constraints. Based on a set of conditions, and Boolean logic and arithmetic operations, unsuitable areas were identified and eliminated. Main constraints for national land-based development included competition over space along KwaZuluNatal Province coastline and restricted access to the coast along the south region of the Northern Cape Province, Wild Coast along the Eastern Cape Province, and Maputaland along KwaZulu-Natal Province. Ten areas along the country coastline were therefore identified as potentially suitable for land-based mariculture. Furthermore, South African abalone ranching potential was found to be limited mostly along the Western and Northern Cape Province's coastline due to the high risk of Paralytic Shellfish Poison (PSP) occurrences, alternative resource use and activities such as commercial fishery, poaching, and conservation. Hence, a total of nine areas along the Northern Cape, Western Cape, and Eastern Cape coastlines were identified as premier areas for abalone ranching. The regional study along the Namaqualand coastline revealed potential conflict between mining activity and mariculture development due to security issues and restricted access to the coast. Land-based mariculture development was confined to the four main coastal urban areas. The highest potential for land-based mariculture was along Port Nolloth and Kleinsee coastlines, whereas marine-based (i.e. abalone ranching) potential was poor along the north part of the coast due to intense marine mining activities, restricted access to the coast, and low kelp bed density. High potential abalone ranching areas were identified south to Kleinsee, and around Port Nolloth. Potential conflict with marine mining activity was minimal since it was localised and not related to kelp bed locations. It was concluded that GIS is a relevant and compatible tool for South African mariculture sector planning. However, future development of GIS as integrated planning tool in mariculture and coastal planning, requires updated spatial data (e.g. recreational activity), and continued interaction among project planners, mariculture specialists and GIS analysts.
- Full Text:
- Date Issued: 2004
- Authors: Shiran-Klotz, Imran
- Date: 2004
- Subjects: Geographic information systems -- South Africa , Fishery management -- Geographic information systems -- South Africa , Mariculture -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5337 , http://hdl.handle.net/10962/d1005870 , Geographic information systems -- South Africa , Fishery management -- Geographic information systems -- South Africa , Mariculture -- South Africa
- Description: The South African coastline has a high potential for mariculture development, particularly in land-based systems, due to excellent water quality, good infrastructure, and relatively cheap land in certain areas. Development of mariculture however, has been slow primarily due to the absence of a national sector development plan to coordinate contribution to development by government, industry and academia. Recent mariculture development plans however, supported by government commitment to stimulate coastal development, offers new opportunity for the sector's growth. These new sector development plans require a multi-disciplinary intensive information base with a strong regional and national spatial component. Geographic Information System (GIS) is a spatial analytical tool, which is capable of handling such large coastal databases and analysing them. Nevertheless, many mariculture GIS planning applications, often developed in isolation and with limited practical use for decision makers, highlighted the need to develop GIS in relation to the mariculture sector development plan. Hence, the aim of this study was to develop and evaluate a GIS for the national and regional mariculture sector planning process in South Africa, in order to identify potentially suitable areas along the South African coastline for mariculture operations. The GIS development in this study was based on the mariculture planning approach developed by PAP/RAe (1996), which recognizes the variations of scales in mariculture planning. Three case studies, representing the diversity of the South African sector plan, were selected to develop the GIS. They included: a national suitability analysis for all land-based culture systems, a specific national analysis on abalone ranching, and a detailed regional analysis of land-based culture and abalone ranching along the Namaqualand coastline. The GIS was developed separately for each case study based on a strategy consisting of six main phases, including: identification of project requirements, developing analytical framework, selection and location of data sources, organization and manipulation of data, analysing data and verifying and evaluation of the outputs. Biophysical, coastal use, and infrastructure criteria were collected, sorted and analysed to identify development constraints. Based on a set of conditions, and Boolean logic and arithmetic operations, unsuitable areas were identified and eliminated. Main constraints for national land-based development included competition over space along KwaZuluNatal Province coastline and restricted access to the coast along the south region of the Northern Cape Province, Wild Coast along the Eastern Cape Province, and Maputaland along KwaZulu-Natal Province. Ten areas along the country coastline were therefore identified as potentially suitable for land-based mariculture. Furthermore, South African abalone ranching potential was found to be limited mostly along the Western and Northern Cape Province's coastline due to the high risk of Paralytic Shellfish Poison (PSP) occurrences, alternative resource use and activities such as commercial fishery, poaching, and conservation. Hence, a total of nine areas along the Northern Cape, Western Cape, and Eastern Cape coastlines were identified as premier areas for abalone ranching. The regional study along the Namaqualand coastline revealed potential conflict between mining activity and mariculture development due to security issues and restricted access to the coast. Land-based mariculture development was confined to the four main coastal urban areas. The highest potential for land-based mariculture was along Port Nolloth and Kleinsee coastlines, whereas marine-based (i.e. abalone ranching) potential was poor along the north part of the coast due to intense marine mining activities, restricted access to the coast, and low kelp bed density. High potential abalone ranching areas were identified south to Kleinsee, and around Port Nolloth. Potential conflict with marine mining activity was minimal since it was localised and not related to kelp bed locations. It was concluded that GIS is a relevant and compatible tool for South African mariculture sector planning. However, future development of GIS as integrated planning tool in mariculture and coastal planning, requires updated spatial data (e.g. recreational activity), and continued interaction among project planners, mariculture specialists and GIS analysts.
- Full Text:
- Date Issued: 2004
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