- Title
- The use of experimental design for the development of a capillary zone electrophoresis method for the quantitation of captopril
- Creator
- Mukozhiwa, S Y, Khamanga, Sandile M, Walker, Roderick B
- Subject
- To be catalogued
- Date
- 2017
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/183828
- Identifier
- vital:44073
- Identifier
- xlink:href="https://doi.org/10.1691/ph.2017.7071"
- Description
- A capillary zone electrophoresis (CZE) method for the quantitation of captopril (CPT) using UV detection was developed. Influence of electrolyte concentration and system variables on electrophoretic separation was evaluated and a central composite design (CCD) was used to optimize the method. Variables investigated were pH, molarity, applied voltage and capillary length. The influence of sodium metabisulphite on the stability of test solutions was also investigated. The use of sodium metabisulphite prevented degradation of CPT over 24 hours. A fused uncoated silica capillary of 67.5cm total and 57.5 cm effective length was used for analysis. The applied voltage and capillary length affected the migration time of CPT significantly. A 20 mM phosphate buffer adjusted to pH 7.0 was used as running buffer and an applied voltage of 23.90 kV was suitable to effect a separation. The optimized electrophoretic conditions produced sharp, well-resolved peaks for CPT and sodium metabisulphite. Linear regression analysis of the response for CPT standards revealed the method was linear (R2 = 0.9995) over the range 5-70 μg/mL. The limits of quantitation and detection were 5 and 1.5 μg/mL. A simple, rapid and reliable CZE method has been developed and successfully applied to the analysis of commercially available CPT products.
- Format
- computer, online resource, application/pdf, 1 online resource (7 pages), pdf
- Publisher
- Avoxa - Mediengruppe Deutscher Apotheker GmbH
- Language
- English
- Relation
- Die Pharmazie-An International Journal of Pharmaceutical Sciences, Mukozhiwa, S.Y., Khamanga, S.M.M. and Walker, R.B., 2017. The use of experimental design for the development of a capillary zone electrophoresis method for the quantitation of captopril. Die Pharmazie-An International Journal of Pharmaceutical Sciences, 72(9), pp.518-524, Die Pharmazie-An International Journal of Pharmaceutical Sciences volume 72 number 9 p.518 May 2017 0031-7144
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the AVOXA Mediengruppe Deutscher Apotheker Copyright, Limitation of Liability for Content Statement (https://avoxa.de/agb/)
- Rights
- Closed Access
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Collections
Walker, Roderick Bryan (Prof)
Khamanga, Sandile S M (Assoc Prof)
RU Department of Pharmacy
SDG 03. Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details | SOURCE1 | The use of experimental design for the development of a capillary zone electrophoresis method for the quantitation of captopril.pdf | 747 KB | Adobe Acrobat PDF | View Details |