- Title
- Interaction of silver nanoparticles with catechol O-methyltransferase: Spectroscopic and simulation analyses
- Creator
- Usman, Aminu, Lobb, Kevin A, Pletschke, Brett I, Whiteley, Christopher G, Wilhelmi, Brendan S
- Subject
- To be catalogued
- Date
- 2021
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/451095
- Identifier
- vital:75018
- Identifier
- xlink:href=" https://doi.org/10.1016/j.bbrep.2021.101013"
- Description
- Catechol O-methyltransferase, an enzyme involved in the metabolism of catechol containing compounds, catalyzes the transfer of a methyl group between S-adenosylmethionine and the hydroxyl groups of the catechol. Furthermore it is considered a potential drug target for Parkinson’s disease as it metabolizes the drug levodopa. Consequently inhibitors of the enzyme would increase levels of levodopa. In this study, absorption, fluorescence and infrared spectroscopy as well as computational simulation studies investigated human soluble catechol Omethyltransferase interaction with silver nanoparticles. The nanoparticles form a corona with the enzyme and quenches the fluorescence of Trp143. This amino acid maintains the correct structural orientation for the catechol ring during catalysis through a static mechanism supported by a non-fluorescent fluorophore–nanoparticle complex. The enzyme has one binding site for AgNPs in a thermodynamically spontaneous binding driven by electrostatic interactions as confirmed by negative ΔG and ΔH and positive ΔS values. Fourier transform infrared spectroscopy within the amide I region of the enzyme indicated that the interaction causes relaxation of its β− structures, while simulation studies indicated the involvement of six polar amino acids. These findings suggest AgNPs influence the catalytic activity of catechol O-methyltransferase, and therefore have potential in controlling the activity of the enzyme.
- Format
- computer, online resource, application/pdf, 1 online resource (9 pages), pdf
- Publisher
- Elsevier
- Language
- English
- Relation
- Biochemistry and Biophysics Reports, Usman, A., Lobb, K., Pletschke, B.I., Whiteley, C.G. and Wilhelmi, B.S., 2021. Interaction of silver nanoparticles with catechol O-methyltransferase: Spectroscopic and simulation analyses. Biochemistry and Biophysics Reports, 26, p.101013, Biochemistry and Biophysics Reports volume 26 p. 101013 2021 2405-5808
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)
- Rights
- Open Access
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- Visitors: 136
- Downloads: 12
Collections
Whiteley, Christopher (Emeritus Prof)
Wilhelmi, Brendan (Dr)
Lobb, Kevin Allan (Assoc Prof)
Pletschke, Brett (Prof)
RU Department of Chemistry
RU Department of Biochemistry, Microbiology and Bioinformatics
SDG 03. Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details | SOURCE1 | Interaction of silver nanoparticles with catechol O-methyltransferase.pdf | 2 MB | Adobe Acrobat PDF | View Details |