- Title
- Optimisation of an electrochemical impedance spectroscopy aptasensor by exploiting quartz crystal microbalance with dissipation signals
- Creator
- Formisanoa, Nello, Jolly, Pawan, Bhalla, Nikhil, Cromhout, Mary, Flanagan, Shane P, Fogel, Ronen, Limson, Janice L, Estrela, Pedro
- Subject
- To be catalogued
- Date
- 2015
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/431699
- Identifier
- vital:72797
- Identifier
- xlink:href="https://doi.org/10.1016/j.snb.2015.05.049"
- Description
- The response of an Electrochemical Impedance Spectroscopy (EIS) sensor using DNA aptamers is affected by many factors, such as DNA density, charge and conformational changes upon DNA-target binding, and buffer conditions. We report here for the first time on the optimisation of an EIS aptamer-based sensor by using Quartz Crystal Microbalance with Dissipation mode (QCM-D). As a case study, we employed a DNA aptamer against Prostate-Specific Antigen (PSA). PSA detection was achieved by functionalising the gold sensor surface via thiol chemistry with different ratios of thiolated-DNA aptamer and 6-mercapto-1-hexanol (MCH) used as spacer molecules. PSA binding efficiency can be monitored by measuring QCM-D signals which not only provide information about the mass of PSA bound on the sensor surface, but also crucial information about the aptamer conformation and layer hydration.
- Format
- computer, online resource, application/pdf, 1 online resource (7pages), pdf
- Publisher
- Elsevier
- Language
- English
- Relation
- Sensors and Actuators B: Chemical, Formisano, N., Jolly, P., Bhalla, N., Cromhout, M., Flanagan, S.P., Fogel, R., Limson, J.L. and Estrela, P., 2015. Optimisation of an electrochemical impedance spectroscopy aptasensor by exploiting quartz crystal microbalance with dissipation signals. Sensors and Actuators B: Chemical, 220, pp.369-375, Sensors and Actuators B: Chemical volume 220 p. 369 2015 0925-4005
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)
- Rights
- Closed Access
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- Visitors: 221
- Downloads: 4
Collections
Fogel, Ronen (Dr)
Limson, Janice (Prof)
RU DST/NRF SARChI for Biotechnology Innovation and Engagement
SDG 03. Good Health and Well-Being
RU Department of Biochemistry, Microbiology and Bioinformatics
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