- Title
- The use of experimental design for the development and validation of an HPLC-ECD method for the quantitation of efavirenz
- Creator
- Makoni, Pedzisai A, Khamanga, Sandile M, Kasongo, Kasongo W, Walker, Roderick B
- Subject
- To be catalogued
- Date
- 2018
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/183556
- Identifier
- vital:44006
- Identifier
- xlink:href="https://doi.org/10.1691/ph.2018.8074"
- Description
- A high performance liquid chromatography with electrochemical detection (HPLC-ECD) method for the quantitation of efavirenz (EFV) was developed, since traditional HPLC-UV methods may be inappropriate, given that EFV undergoes photolytic degradation following exposure to UV light. This work describes the use of response surface methodology (RSM) based on a central composite design (CCD) to develop a stability-indicating HPLC method with pulsed ECD in direct current (DC) mode at an applied potential difference and current of +1400 mV and 1.0 μA for the analysis of EFV. Separation of EFV and imipramine was achieved using a Nova-Pak®C18 cartridge column and a mobile phase of phosphate buffer (pH 4.5): acetonitrile (ACN) (55:45 v/v). Mobile phase pH, buffer molarity, ACN concentration and applied potential difference were investigated. The optimized method produced sharp well resolved peaks for imipramine and EFV with retention times of 3.70 and 8.89 minutes. The calibration curve was linear (R2 = 0.9979) over the range 5-70 μg/mL. Repeatability and intermediate precision ranged between 3.37 and 4.34 % RSD and 1.31 and 4.29 % RSD and accuracy between -0.80 and 4.71 % bias. The LOQ and LOD were 5.0 and 1.5 μg/mL. The method was specific for EFV and was used to analyse EFV in commercially available tablets. The HPLC-ECD method is more suitable for quantitative analysis of EFV than HPLC-UV.
- Format
- computer, online resource, application/pdf, 1 online resource (9 pages), pdf
- Publisher
- Avoxa - Mediengruppe Deutscher Apotheker GmbH
- Language
- English
- Relation
- Die Pharmazie-An International Journal of Pharmaceutical Sciences, Makoni, P.A., Khamanga, S.M., Wa Kasongo, K. and Walker, R.B., 2018. The use of experimental design for the development and validation of an HPLC-ECD method for the quantitation of efavirenz. Die Pharmazie-An International Journal of Pharmaceutical Sciences, 73(10), pp.570-578, Die Pharmazie-An International Journal of Pharmaceutical Sciences volume 73 number 10 p. 570 2018 0031-7144
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the AVOXA Mediengruppe Deutscher Apotheker Copyright, Limitation of Liability for Content Statement (https://avoxa.de/agb/)
- Rights
- Closed Access
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Collections
Walker, Roderick Bryan (Prof)
Makoni, Pedzisai A (Dr)
Khamanga, Sandile S M (Assoc Prof)
RU Department of Pharmacy
SDG 03. Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details | SOURCE1 | The use of experimental design for the development and validation of an HPLC-ECD method for the quantitation of efavirenz.pdf | 1 MB | Adobe Acrobat PDF | View Details |