Synchronisation of breeding in populations of the brown mussel Perna perna on the South Coast of South Africa
- Authors: Ndzipa, Victoria
- Date: 2013-05-28
- Subjects: Mussels -- South Africa , Perna -- South Africa , Perna -- Breeding
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5830 , http://hdl.handle.net/10962/d1007857 , Mussels -- South Africa , Perna -- South Africa , Perna -- Breeding
- Description: The general biology and seasonality of breeding of intertidal populations of the brown mussel Perna perna in South Africa are reasonably well known, but we have little information on variability either within or among populations. Synchronous spawning offers adaptive advantages to externally breeding animals. Firstly, it enhances fertilization rates and therefore the species' reproductive fitness. Secondly, spawning can also be timed to coincide with environmental conditions conducive to larval settlement and development. In addition, synchronisation of spawning will influence the synchrony of settlement. Synchronisation of larval settlement, in turn, has implications for popUlation biology, as highly pulsed settlement is likely to lead to density-dependant mortality of recruits and uncoupling of adult/recruit densities, while poorly synchronised settlement will not. Generally, sea temperature and food availability are considered the key factors underlying the initiation and the duration of the breeding cycle of mussels. However, there are proximate local cues that trigger the proliferation, maturation and release of gametes. In this study, the hypothesis tested is that factors that control food availability affect gonad development and so influence synchrony among populations. Much of the published work on spawning is based on observations of the presence of larvae in the plankton, or on settlement. A more reliable method correlates the sequence of gonad development throughout the year with changes in length-weight relationships, using histology. This study is also designed to investigate temporal differences in the timing of the breeding cycle between sheltered and exposed sites along the south coast of South Africa by histological analysis of the reproductive tissue (the gonad) and by dry weight/shell length regreSSIOns. To do this, these two techniques were applied to six mussel populations at three III ocalities that were separated on scales of about 10-20km. Within each locality, two study sites were .dentified. One was exposed to strong wave action and one was sheltered. A few hundred meters ;eparated these sites. The first technique used length-weight regressions as an indication of mussel ~ondition. Abrupt decreases in the dry body weight of a hypothetical standard animal were taken to indicate periods of spawning. Regressions were assessed for samples of 40 mussels taken from each site at intervals of 4 weeks over 13 months. The results were analysed using a 3-way ANCOV A, with dry weight as the dependent variable, shell length as a covariate, and site, exposure and month, as independent variables. The second approach used the more reliable and detailed method of assessing the annual reproductive cycle using histological sections of the gonad. Histological sections of gonads from thirty female mussels, sampled monthly from each site, were examined in the laboratory. Each gonad was categorized into one of six arbitrary developmental stages based on ovary morphology. Synchrony in spawning was examined by comparison of gonad developmental stages of individuals within and among populations. The data were analysed by 3-way nested ANOV A with mean gonad index for each population as the dependent variable, month as an independent variable and exposure nested in site. The results obtained from both techniques showed strong synchronisation among different popUlations, regardless of the scales at which they were separated. The data also indicated good synchrony within populations and, again among populations, regardless of the degree of exposure. The results also indicated that the gonad condition varied significantly at each site, exposure level and month. However, there were significant interactions among these three factors. This means that on a broader seasonal scale the six mussel populations were reasonably synchronised, but on finer monthly scales, there were temporal differences in duration of gametogenic events. The implication is that ovary development is cued by environmental factor(s) that operate on scales of at least 7-20 km rather than more localised events that may affect food availablity either through aggregation of food (local hydrography at different localities) or food delivery to the shore (degree of wave action at different sites.) , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Authors: Ndzipa, Victoria
- Date: 2013-05-28
- Subjects: Mussels -- South Africa , Perna -- South Africa , Perna -- Breeding
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5830 , http://hdl.handle.net/10962/d1007857 , Mussels -- South Africa , Perna -- South Africa , Perna -- Breeding
- Description: The general biology and seasonality of breeding of intertidal populations of the brown mussel Perna perna in South Africa are reasonably well known, but we have little information on variability either within or among populations. Synchronous spawning offers adaptive advantages to externally breeding animals. Firstly, it enhances fertilization rates and therefore the species' reproductive fitness. Secondly, spawning can also be timed to coincide with environmental conditions conducive to larval settlement and development. In addition, synchronisation of spawning will influence the synchrony of settlement. Synchronisation of larval settlement, in turn, has implications for popUlation biology, as highly pulsed settlement is likely to lead to density-dependant mortality of recruits and uncoupling of adult/recruit densities, while poorly synchronised settlement will not. Generally, sea temperature and food availability are considered the key factors underlying the initiation and the duration of the breeding cycle of mussels. However, there are proximate local cues that trigger the proliferation, maturation and release of gametes. In this study, the hypothesis tested is that factors that control food availability affect gonad development and so influence synchrony among populations. Much of the published work on spawning is based on observations of the presence of larvae in the plankton, or on settlement. A more reliable method correlates the sequence of gonad development throughout the year with changes in length-weight relationships, using histology. This study is also designed to investigate temporal differences in the timing of the breeding cycle between sheltered and exposed sites along the south coast of South Africa by histological analysis of the reproductive tissue (the gonad) and by dry weight/shell length regreSSIOns. To do this, these two techniques were applied to six mussel populations at three III ocalities that were separated on scales of about 10-20km. Within each locality, two study sites were .dentified. One was exposed to strong wave action and one was sheltered. A few hundred meters ;eparated these sites. The first technique used length-weight regressions as an indication of mussel ~ondition. Abrupt decreases in the dry body weight of a hypothetical standard animal were taken to indicate periods of spawning. Regressions were assessed for samples of 40 mussels taken from each site at intervals of 4 weeks over 13 months. The results were analysed using a 3-way ANCOV A, with dry weight as the dependent variable, shell length as a covariate, and site, exposure and month, as independent variables. The second approach used the more reliable and detailed method of assessing the annual reproductive cycle using histological sections of the gonad. Histological sections of gonads from thirty female mussels, sampled monthly from each site, were examined in the laboratory. Each gonad was categorized into one of six arbitrary developmental stages based on ovary morphology. Synchrony in spawning was examined by comparison of gonad developmental stages of individuals within and among populations. The data were analysed by 3-way nested ANOV A with mean gonad index for each population as the dependent variable, month as an independent variable and exposure nested in site. The results obtained from both techniques showed strong synchronisation among different popUlations, regardless of the scales at which they were separated. The data also indicated good synchrony within populations and, again among populations, regardless of the degree of exposure. The results also indicated that the gonad condition varied significantly at each site, exposure level and month. However, there were significant interactions among these three factors. This means that on a broader seasonal scale the six mussel populations were reasonably synchronised, but on finer monthly scales, there were temporal differences in duration of gametogenic events. The implication is that ovary development is cued by environmental factor(s) that operate on scales of at least 7-20 km rather than more localised events that may affect food availablity either through aggregation of food (local hydrography at different localities) or food delivery to the shore (degree of wave action at different sites.) , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
Geotectonic controls on primary diamond deposits : a review of exploration criteria
- Authors: Hannon, Camille
- Date: 2013-05-23
- Subjects: Diamonds , Geology, Structural , Diamond deposits , Kimberlite
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5047 , http://hdl.handle.net/10962/d1007810 , Diamonds , Geology, Structural , Diamond deposits , Kimberlite
- Description: The origin of diamonds, their preservation and transport to the surface have been important issues over the last decades after the acknowledgement that diamonds are xenocrysts in the host kimberlites and after the discovery of new transport media such as lamproites. Different types of diamonds -E-type diamonds, P-type diamonds- and different types of hosts - Eclogites, Peridotites- have been distinguished. Each type corresponds to particular formation criteria. Ecogitic Diamonds are mostly related to subduction processes, whereas more uncertainties remain regarding the formation of Peridotitic Diamonds. Komatiite extraction and subduction of graphite-bearing serpentinites have been proposed as the more likely processes involved in their formation. A typical mantle signature for diamonds implies a thick, cool, reduced lithosphere. The keel-shape model is the most popular. Archaean cratons are therefore the most promising exploration target and area selection will expect to follow the Clifford's Rule. However, the evidence of cratonic areas hidden under younger formations · through seismic profiles and the discovery of diamond structurally trapped outside their stability field, have increased the potential of diamondiferous areas. Preservation of diamonds inside the lithosphere requires that the mantleroot remains insulated against excessive reheating and tectonic reworking. Mantle-root friendly and mantle-root destructive structures are distinguished. Small-size cratons are usually the most promising exploration targets. Transport of diamonds to the surface is dependant on' the same criteria of preservation. Only kimberlites and lamproites have been recognized as efficient transport media. Their ascent to the surface is conditioned by a multitude of parameters, amongst them the nature of the magma, the speed of ascent, the presence of pre-existing structures in the crust and the availability of ground water in the near-surface environment. The origin of kimberlite magma probably lies near the transition zone. Mixtures of depleted asthenospheric · sources and metasomatically enriched and possibly subducted materials are likely to be at the origin of the different kimberlite magmas. Kimberlite magmatism correlates generally in time with global tectonic events, triggered by either plume activity or by subduction processes, depending of the tectonic school of thought. Kimberlite alignments have been interpreted as hotspot tracks, and kimberlite magmas as volatile-rich melts issued from the remaining plume tail. The plume head produces flood-basalts in an adjacent "thinspot" of the lithosphere, generally on the edges of the cratons. Kimberlite and lamproite ascent to the surface are unconditionally influenced by regional structures. Rift structures, ring structures, transform faults, suture zones and deep-seated faults have been mentioned as controlling or accompanying features of kimberlite magmatism. Nearsurface emplacement constraints are better understood and the ultimate shape of the intrusion(s) depends on the nature of the country rocks, the availability of ground water and the near-surface faulting pattern. The recent discovery of "fissure" kimberlites is one of the more important breakthroughs of the last decade. With a better understanding of the processes involved in diamond formation, preservation and of kimberlite emplacement, major diamond discoveries have recently increased on all the continents. Successful diamond exploration requires today an integration of all geophysical, petrologic, geochemical and structural information available. The particular study of the northwestern Australian lamproite and kimberlite fields, the Brazilian kimberlites, the easternNorth American kimberlite fields, the Lac de Gras kimberlite field, the South African rich kimberlite provinces, and the Yakutian kimberlite fields provide concrete examples of the geotectonic controls on primary diamond deposits. Area selection criteria based on the previous models and examples, are expected to yield to many more discoveries in the coming years. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Authors: Hannon, Camille
- Date: 2013-05-23
- Subjects: Diamonds , Geology, Structural , Diamond deposits , Kimberlite
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5047 , http://hdl.handle.net/10962/d1007810 , Diamonds , Geology, Structural , Diamond deposits , Kimberlite
- Description: The origin of diamonds, their preservation and transport to the surface have been important issues over the last decades after the acknowledgement that diamonds are xenocrysts in the host kimberlites and after the discovery of new transport media such as lamproites. Different types of diamonds -E-type diamonds, P-type diamonds- and different types of hosts - Eclogites, Peridotites- have been distinguished. Each type corresponds to particular formation criteria. Ecogitic Diamonds are mostly related to subduction processes, whereas more uncertainties remain regarding the formation of Peridotitic Diamonds. Komatiite extraction and subduction of graphite-bearing serpentinites have been proposed as the more likely processes involved in their formation. A typical mantle signature for diamonds implies a thick, cool, reduced lithosphere. The keel-shape model is the most popular. Archaean cratons are therefore the most promising exploration target and area selection will expect to follow the Clifford's Rule. However, the evidence of cratonic areas hidden under younger formations · through seismic profiles and the discovery of diamond structurally trapped outside their stability field, have increased the potential of diamondiferous areas. Preservation of diamonds inside the lithosphere requires that the mantleroot remains insulated against excessive reheating and tectonic reworking. Mantle-root friendly and mantle-root destructive structures are distinguished. Small-size cratons are usually the most promising exploration targets. Transport of diamonds to the surface is dependant on' the same criteria of preservation. Only kimberlites and lamproites have been recognized as efficient transport media. Their ascent to the surface is conditioned by a multitude of parameters, amongst them the nature of the magma, the speed of ascent, the presence of pre-existing structures in the crust and the availability of ground water in the near-surface environment. The origin of kimberlite magma probably lies near the transition zone. Mixtures of depleted asthenospheric · sources and metasomatically enriched and possibly subducted materials are likely to be at the origin of the different kimberlite magmas. Kimberlite magmatism correlates generally in time with global tectonic events, triggered by either plume activity or by subduction processes, depending of the tectonic school of thought. Kimberlite alignments have been interpreted as hotspot tracks, and kimberlite magmas as volatile-rich melts issued from the remaining plume tail. The plume head produces flood-basalts in an adjacent "thinspot" of the lithosphere, generally on the edges of the cratons. Kimberlite and lamproite ascent to the surface are unconditionally influenced by regional structures. Rift structures, ring structures, transform faults, suture zones and deep-seated faults have been mentioned as controlling or accompanying features of kimberlite magmatism. Nearsurface emplacement constraints are better understood and the ultimate shape of the intrusion(s) depends on the nature of the country rocks, the availability of ground water and the near-surface faulting pattern. The recent discovery of "fissure" kimberlites is one of the more important breakthroughs of the last decade. With a better understanding of the processes involved in diamond formation, preservation and of kimberlite emplacement, major diamond discoveries have recently increased on all the continents. Successful diamond exploration requires today an integration of all geophysical, petrologic, geochemical and structural information available. The particular study of the northwestern Australian lamproite and kimberlite fields, the Brazilian kimberlites, the easternNorth American kimberlite fields, the Lac de Gras kimberlite field, the South African rich kimberlite provinces, and the Yakutian kimberlite fields provide concrete examples of the geotectonic controls on primary diamond deposits. Area selection criteria based on the previous models and examples, are expected to yield to many more discoveries in the coming years. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
A comparative analysis of Java and .NET mobile development environments for supporting mobile services
- Authors: Zhao, Xiaogeng
- Date: 2003 , 2013-05-23
- Subjects: Microsoft .NET , Java (Computer program language) , Mobile computing , Wireless communication systems
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4578 , http://hdl.handle.net/10962/d1003064 , Microsoft .NET , Java (Computer program language) , Mobile computing , Wireless communication systems
- Description: With the rapid development of wireless technologies, traditional mobile devices, such as pagers and cellular phones, have evolved from a purely communications and messaging-oriented medium to one that supports mobile data communication in general and acts as an application platform. As shown in a recent survey conducted by MDA, easy access to the present-day wireless Internet has resulted in mobile devices gaining more and more attention and popularity. The growth of and demand for mobile Web applications is expected to increase rapidly in the near future, as a range of software companies and mobile device manufacturers release increasingly accessible tools for creating mobile Web application and services. From a variety of possible development environments of this kind, the author has selected and examined two leading contenders, the J2ME and the Microsoft .NET mobile Web application development environments. This document reports the product life cycle of pilot mobile web applications, designed and implemented in each host environment in tum. A feature-by-feature investigation and comparison of the J2ME and .NET environments was carried out, covering the range of issues necessary for a complete mobile Web application development life cycle. The resulting analysis addresses features and efficiencies of the application development environment and the target deployment environment, the degree to which the resultant services are compatible on a variety of platforms, and the ease with which applications can be designed to be extensible. The thesis offers an objective evaluation of the J2ME and the .NET mobile development environments, which highlights their strengths and weaknesses, and suggests guidelines for designing, creating, and deploying high quality mobile Web applications. The research uncovers no clear winner across all categories assessed. J2ME currently favours situations in which bandwidth is limited and client side processing power is relatively sufficient, it exerts the processing power of mobile devices over distributed network environments. .NET requires a less constrained network throughput, but performs adequately on clients with more limited processing power, supports a more diverse target platform range, and offers a more efficient, in terms of development time, development environment. Both technologies are likely to receive significant user support for some time. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Authors: Zhao, Xiaogeng
- Date: 2003 , 2013-05-23
- Subjects: Microsoft .NET , Java (Computer program language) , Mobile computing , Wireless communication systems
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4578 , http://hdl.handle.net/10962/d1003064 , Microsoft .NET , Java (Computer program language) , Mobile computing , Wireless communication systems
- Description: With the rapid development of wireless technologies, traditional mobile devices, such as pagers and cellular phones, have evolved from a purely communications and messaging-oriented medium to one that supports mobile data communication in general and acts as an application platform. As shown in a recent survey conducted by MDA, easy access to the present-day wireless Internet has resulted in mobile devices gaining more and more attention and popularity. The growth of and demand for mobile Web applications is expected to increase rapidly in the near future, as a range of software companies and mobile device manufacturers release increasingly accessible tools for creating mobile Web application and services. From a variety of possible development environments of this kind, the author has selected and examined two leading contenders, the J2ME and the Microsoft .NET mobile Web application development environments. This document reports the product life cycle of pilot mobile web applications, designed and implemented in each host environment in tum. A feature-by-feature investigation and comparison of the J2ME and .NET environments was carried out, covering the range of issues necessary for a complete mobile Web application development life cycle. The resulting analysis addresses features and efficiencies of the application development environment and the target deployment environment, the degree to which the resultant services are compatible on a variety of platforms, and the ease with which applications can be designed to be extensible. The thesis offers an objective evaluation of the J2ME and the .NET mobile development environments, which highlights their strengths and weaknesses, and suggests guidelines for designing, creating, and deploying high quality mobile Web applications. The research uncovers no clear winner across all categories assessed. J2ME currently favours situations in which bandwidth is limited and client side processing power is relatively sufficient, it exerts the processing power of mobile devices over distributed network environments. .NET requires a less constrained network throughput, but performs adequately on clients with more limited processing power, supports a more diverse target platform range, and offers a more efficient, in terms of development time, development environment. Both technologies are likely to receive significant user support for some time. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
A review of the Kalahari group: an aid to Kimberlite exploration in this medium
- Authors: Williams, Clint
- Date: 2003 , 2013-05-23
- Subjects: Kimberlite -- Kalahari Desert , Sedimentation and deposition -- Kalahari Desert
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4916 , http://hdl.handle.net/10962/d1003216 , Kimberlite -- Kalahari Desert , Sedimentation and deposition -- Kalahari Desert
- Description: The Kalahari Group sediments cover vast portions of the Archean Kaapvaal and Congo cratons that are considered highly prospective for economic kimberlites. In southern Africa, the term Kalahari refers to a structural basin, a group of Cretaceous to recent terrestrial continental sediments and an ill-defined desert, all of which have been grouped together as the Mega Kalahari by Thomas and Shaw (1993). The Mega Kalahari grouping includes sediments stretching from South Africa in the south to the Democratic Republic of Congo in the north, and from eastern Namibia to western Zimbabwe. This sand sea, at 2.5 million km², is the largest on earth and presents significant obstacles and challenges to the kimberlite explorationist attempting to locate bedrock-hosted diamondiferous kimberlite bodies. The Mega Kalahari sediments represent an ancient depositional environment with a complex history in which the stratigraphy and age of the deposits are not particularly well constrained or understood. Low fossil content, limited exposure, poor differentiation of the dominant surficial Kalahari Sand and a limited comprehension of an extensive duricrust suite has delayed the understanding of the sedimentological and environmental history of the basin. This sequence of sediments has accumulated and evolved through fluvio-deltaic, aeolian and groundwater processes, with characteristics due to primary deposition and subsequent modification being difficult to distinguish. Deposition in the Kalahari Basin has been subject to tectonic influences, changes in drainage directions and source areas of sediments, river capture and numerous large and small climatic fluctuations both in the basin and surrounding areas. It bears the imprint of recurring cycles during which the same sediments were reworked, sometimes by different agencies, all of which exacerbate attempts to correlate sedimentary units across the sequence. The Mega Kalahari is a series of contiguous Phanerozoic sedimentary basins situated within the African Superswell. The Superswell has dominated the gross geomorphology of southern Africa and contributed significantly to the present character of the Mega Kalahari and the evolution of the drainage systems. Overall, the tectonic framework established in southern Africa by the division of Gondwanaland led to the creation of a dual drainage system, with the hingeline acting as a watershed between a coastally-orientated exoreic system and an endoreic system draining into the interior. Deposition of sediments started in the late Cretaceous. Neo-tectonic activity expressed in the rifting in central Botswana, further influenced sedimentation rates and exerted a strong control over paleo-drainage directions. This revIew presents the complexities of the Kalahari cover sequence. The most Important geomorphological and sedimentary factors to be considered when designing and implementing kimberlite exploration programs within the Mega Kalahari environment are outlined and discussed. New data from exploration drilling programs are presented on the thickness of the Kalahari within portions of northern Namibia, western Zambia and Botswana. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Authors: Williams, Clint
- Date: 2003 , 2013-05-23
- Subjects: Kimberlite -- Kalahari Desert , Sedimentation and deposition -- Kalahari Desert
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4916 , http://hdl.handle.net/10962/d1003216 , Kimberlite -- Kalahari Desert , Sedimentation and deposition -- Kalahari Desert
- Description: The Kalahari Group sediments cover vast portions of the Archean Kaapvaal and Congo cratons that are considered highly prospective for economic kimberlites. In southern Africa, the term Kalahari refers to a structural basin, a group of Cretaceous to recent terrestrial continental sediments and an ill-defined desert, all of which have been grouped together as the Mega Kalahari by Thomas and Shaw (1993). The Mega Kalahari grouping includes sediments stretching from South Africa in the south to the Democratic Republic of Congo in the north, and from eastern Namibia to western Zimbabwe. This sand sea, at 2.5 million km², is the largest on earth and presents significant obstacles and challenges to the kimberlite explorationist attempting to locate bedrock-hosted diamondiferous kimberlite bodies. The Mega Kalahari sediments represent an ancient depositional environment with a complex history in which the stratigraphy and age of the deposits are not particularly well constrained or understood. Low fossil content, limited exposure, poor differentiation of the dominant surficial Kalahari Sand and a limited comprehension of an extensive duricrust suite has delayed the understanding of the sedimentological and environmental history of the basin. This sequence of sediments has accumulated and evolved through fluvio-deltaic, aeolian and groundwater processes, with characteristics due to primary deposition and subsequent modification being difficult to distinguish. Deposition in the Kalahari Basin has been subject to tectonic influences, changes in drainage directions and source areas of sediments, river capture and numerous large and small climatic fluctuations both in the basin and surrounding areas. It bears the imprint of recurring cycles during which the same sediments were reworked, sometimes by different agencies, all of which exacerbate attempts to correlate sedimentary units across the sequence. The Mega Kalahari is a series of contiguous Phanerozoic sedimentary basins situated within the African Superswell. The Superswell has dominated the gross geomorphology of southern Africa and contributed significantly to the present character of the Mega Kalahari and the evolution of the drainage systems. Overall, the tectonic framework established in southern Africa by the division of Gondwanaland led to the creation of a dual drainage system, with the hingeline acting as a watershed between a coastally-orientated exoreic system and an endoreic system draining into the interior. Deposition of sediments started in the late Cretaceous. Neo-tectonic activity expressed in the rifting in central Botswana, further influenced sedimentation rates and exerted a strong control over paleo-drainage directions. This revIew presents the complexities of the Kalahari cover sequence. The most Important geomorphological and sedimentary factors to be considered when designing and implementing kimberlite exploration programs within the Mega Kalahari environment are outlined and discussed. New data from exploration drilling programs are presented on the thickness of the Kalahari within portions of northern Namibia, western Zambia and Botswana. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
An empirical, in-depth investigation into service creation in H.323 Version 4 Networks
- Authors: Penton, Jason Barry
- Date: 2003 , 2013-05-24
- Subjects: Computer programming , Computer networks , Computer network protocols
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4681 , http://hdl.handle.net/10962/d1007637 , Computer programming , Computer networks , Computer network protocols
- Description: Over the past few years there has been an increasing tendency to carry voice on IP networks as opposed to the PSTN and other switched circuit networks. Initially this trend was favoured due to reduced costs but occurred at the expense of sacrificing the quality of the voice communications. Switched circuit networks have therefore remained the preferred carrier-grade voice communication network, but this is again changing. The advancement in improved quality of service (QoS) of real-time traffic on the IP network is a contributing factor to the anticipated future of the IP network supplying carrier-grade voice communications. Another contributing factor is the possibility of creating a new range of innovative, state-of-the-art telephony and communications services that acquire leverage through the intelligence and flexibility of the IP network. The latter has yet to be fully explored. Various protocols exist that facilitate the transport of voice and other media on IP networks. The most well known and widely supported of these is H.323. This work presents and discusses H.323 version 4 service creation. The work also categorises the various H.323 services and presents the mechanisms provided by H.323 version 4 that have facilitated the development of the three services I have developed, EmailReader, Telgo323 and CANS.
- Full Text:
- Authors: Penton, Jason Barry
- Date: 2003 , 2013-05-24
- Subjects: Computer programming , Computer networks , Computer network protocols
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4681 , http://hdl.handle.net/10962/d1007637 , Computer programming , Computer networks , Computer network protocols
- Description: Over the past few years there has been an increasing tendency to carry voice on IP networks as opposed to the PSTN and other switched circuit networks. Initially this trend was favoured due to reduced costs but occurred at the expense of sacrificing the quality of the voice communications. Switched circuit networks have therefore remained the preferred carrier-grade voice communication network, but this is again changing. The advancement in improved quality of service (QoS) of real-time traffic on the IP network is a contributing factor to the anticipated future of the IP network supplying carrier-grade voice communications. Another contributing factor is the possibility of creating a new range of innovative, state-of-the-art telephony and communications services that acquire leverage through the intelligence and flexibility of the IP network. The latter has yet to be fully explored. Various protocols exist that facilitate the transport of voice and other media on IP networks. The most well known and widely supported of these is H.323. This work presents and discusses H.323 version 4 service creation. The work also categorises the various H.323 services and presents the mechanisms provided by H.323 version 4 that have facilitated the development of the three services I have developed, EmailReader, Telgo323 and CANS.
- Full Text:
An integrated economic developmental appraisal of the South African mariculture industry
- Authors: Hepburn, Bruce
- Date: 2003
- Subjects: Mariculture -- South Africa -- Economic aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:941 , http://hdl.handle.net/10962/d1002674 , Mariculture -- South Africa -- Economic aspects
- Description: Current coastal legislative and institutional reformulation has effectively established new principles for the promotion of sustainable, co-ordinated integrated coastal development to be achieved through facilitatory, co-operative management mechanisms. In accordance, collaborative expansion and diversification of mariculture has been identified as a strategic mechanism for realising sustainable coastal development. Present limited foundational understanding regarding the emergent commercial contingent’s economic-institutional structure has constrained the effectiveness of current centralised broad objective formulation based planning techniques. By drawing upon findings of the 2001 National Mariculture Baseline survey, key economic development and institutional components requiring dedicated attention for further realising industry’s inherent growth potential to supply domestic and growing global aquaculture markets are examined in an inductive explorative framework. Attention is also directed towards promoting realisation of collaborative mariculture development initiatives to redress dualistic development disparities in previously marginalised coastal localities. Finally, synthesis between traditional bureaucratic centralised co-ordinated planning and regionalised decentralised implementation orientated capacity building frameworks displaying a greater relevant stakeholder participatory ethos are examined.
- Full Text:
- Authors: Hepburn, Bruce
- Date: 2003
- Subjects: Mariculture -- South Africa -- Economic aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:941 , http://hdl.handle.net/10962/d1002674 , Mariculture -- South Africa -- Economic aspects
- Description: Current coastal legislative and institutional reformulation has effectively established new principles for the promotion of sustainable, co-ordinated integrated coastal development to be achieved through facilitatory, co-operative management mechanisms. In accordance, collaborative expansion and diversification of mariculture has been identified as a strategic mechanism for realising sustainable coastal development. Present limited foundational understanding regarding the emergent commercial contingent’s economic-institutional structure has constrained the effectiveness of current centralised broad objective formulation based planning techniques. By drawing upon findings of the 2001 National Mariculture Baseline survey, key economic development and institutional components requiring dedicated attention for further realising industry’s inherent growth potential to supply domestic and growing global aquaculture markets are examined in an inductive explorative framework. Attention is also directed towards promoting realisation of collaborative mariculture development initiatives to redress dualistic development disparities in previously marginalised coastal localities. Finally, synthesis between traditional bureaucratic centralised co-ordinated planning and regionalised decentralised implementation orientated capacity building frameworks displaying a greater relevant stakeholder participatory ethos are examined.
- Full Text:
An investigation into the application of the IEEE 1394 high performance serial bus to sound installation contro
- Authors: Klinkradt, Bradley Hugh
- Date: 2003 , 2013-05-24
- Subjects: Digital electronics , Sound -- Recording and reproducing -- Digital techniques , Music -- Data processing , Computer sound processing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4612 , http://hdl.handle.net/10962/d1004899 , Digital electronics , Sound -- Recording and reproducing -- Digital techniques , Music -- Data processing , Computer sound processing
- Description: This thesis investigates the feasibility of using existing IP-based control and monitoring protocols within professional audio installations utilising IEEE 1394 technology. Current control and monitoring technologies are examined, and the characteristics common to all are extracted and compiled into an object model. This model forms the foundation for a set of evaluation criteria against which current and future control and monitoring protocols may be measured. Protocols considered include AV/C, MIDI, QSC-24, and those utilised within the UPnP architecture. As QSC-24 and the UPnP architecture are IP-based, the facilities required to transport IP datagrams over the IEEE 1394 bus are investigated and implemented. Example QSC-24 and UPnP architecture implementations are described, which permit the control and monitoring of audio devices over the IEEE 1394 network using these IP-based technologies. The way forward for the control and monitoring of professional audio devices within installations is considered, and recommendations are provided. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Authors: Klinkradt, Bradley Hugh
- Date: 2003 , 2013-05-24
- Subjects: Digital electronics , Sound -- Recording and reproducing -- Digital techniques , Music -- Data processing , Computer sound processing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4612 , http://hdl.handle.net/10962/d1004899 , Digital electronics , Sound -- Recording and reproducing -- Digital techniques , Music -- Data processing , Computer sound processing
- Description: This thesis investigates the feasibility of using existing IP-based control and monitoring protocols within professional audio installations utilising IEEE 1394 technology. Current control and monitoring technologies are examined, and the characteristics common to all are extracted and compiled into an object model. This model forms the foundation for a set of evaluation criteria against which current and future control and monitoring protocols may be measured. Protocols considered include AV/C, MIDI, QSC-24, and those utilised within the UPnP architecture. As QSC-24 and the UPnP architecture are IP-based, the facilities required to transport IP datagrams over the IEEE 1394 bus are investigated and implemented. Example QSC-24 and UPnP architecture implementations are described, which permit the control and monitoring of audio devices over the IEEE 1394 network using these IP-based technologies. The way forward for the control and monitoring of professional audio devices within installations is considered, and recommendations are provided. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
An investigation into the feeding biology and factors influencing the population dynamics of Terebrasabella heterouncinata (Polychaeta: Sabellidae), a problematic tube-dwelling polychaete in farmed abalone in South Africa
- Authors: Chalmers, Russell
- Date: 2003
- Subjects: Polychaeta -- South Africa , Sabellidae -- South Africa , Abalones -- Feeding and feeds -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5291 , http://hdl.handle.net/10962/d1005136 , Polychaeta -- South Africa , Sabellidae -- South Africa , Abalones -- Feeding and feeds -- South Africa
- Description: Since the discovery of a new species of sabellid worm in 1993, the Californian and South African abalone industries have experienced increasing shell breakage and reduced growth rates of abalone, resulting in reduced market value of product. This polychaete worm can reach very high infestation levels. It was described as Terebrasabella heterouncinata in 1999, having originated from the South African coastline where it had been observed on several wild mollusc species. Although not a shell borer, it occupies tubes on the growing edge which are formed by deposition of nacreous shell over sabellid larvae by the host abalone. Heavy sabellid infestation levels have placed the economic viability of several South African farms under threat. Past research methods have not been successful in establishing effective means of eradication or control, partly due to the lack of knowledge of basic biological information on this species. This study forms the first part of an ongoing research project into the biology of terebrasabella heterouncinata. Abalone farms in South African utilise two main diets in abalone culture. An artificial formulated feed, Abfeed, is produced in South Africa and is used by the majority of farms as it increases abalone growth rate. Kelp, a natural diet of abalone, is harvested and also used as a feed. Farmers have observed higher sabellid infestation levels on Abfeed-fed abalone and suspect that diet may be an important factor influencing sabellid infestation. Abalone stocking density has also been identified as a potential factor influencing sabellid population growth on commercial farms. In order to better understand sabellid nutrition, the feeding behaviour of the sabellid in response to suspended solids of various particle size classes from both abalone diets were investigated. Both the effect of abalone diet and stocking density and their effects on sabellid morphometrics were quantified. The proximate composition and particle size distribution of suspended-solids present within kelp and Abfeed abalone raceways were also quantified. Results indicate that sabellid feeding follows no structured behavioural pattern for both abalone diets. The quantity of suspended solids in kelp raceways was greater than in Abfeed raceways (p<0.02) while there was no difference in particulate level between three abalone stocking densities (p>0.05). Protein (p<0.0001) and energy (p<0.0.1) levels of suspended solids were significantly greater in raceways dedicated to feeding Abfeed. The infestation level (number of tubes/centimetre on the grwoing edge) was significantly higher (p<0.001) in Abfeed-fed abalone. The morphometrics of the sabellids indicated that sabellids from Abfeed-fed abalone were larger in various body measurements: length (p<0.00001); neck width (p<0.001); base width (p<0.001); and surface area (p<0.001). This study suggests that abalone diet has an important influence on the infestation level and size of sabellids and that this is likely to be due to the fragmentation and leaching of nutrients from the artificially enriched commercial abalone diet.
- Full Text:
- Authors: Chalmers, Russell
- Date: 2003
- Subjects: Polychaeta -- South Africa , Sabellidae -- South Africa , Abalones -- Feeding and feeds -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5291 , http://hdl.handle.net/10962/d1005136 , Polychaeta -- South Africa , Sabellidae -- South Africa , Abalones -- Feeding and feeds -- South Africa
- Description: Since the discovery of a new species of sabellid worm in 1993, the Californian and South African abalone industries have experienced increasing shell breakage and reduced growth rates of abalone, resulting in reduced market value of product. This polychaete worm can reach very high infestation levels. It was described as Terebrasabella heterouncinata in 1999, having originated from the South African coastline where it had been observed on several wild mollusc species. Although not a shell borer, it occupies tubes on the growing edge which are formed by deposition of nacreous shell over sabellid larvae by the host abalone. Heavy sabellid infestation levels have placed the economic viability of several South African farms under threat. Past research methods have not been successful in establishing effective means of eradication or control, partly due to the lack of knowledge of basic biological information on this species. This study forms the first part of an ongoing research project into the biology of terebrasabella heterouncinata. Abalone farms in South African utilise two main diets in abalone culture. An artificial formulated feed, Abfeed, is produced in South Africa and is used by the majority of farms as it increases abalone growth rate. Kelp, a natural diet of abalone, is harvested and also used as a feed. Farmers have observed higher sabellid infestation levels on Abfeed-fed abalone and suspect that diet may be an important factor influencing sabellid infestation. Abalone stocking density has also been identified as a potential factor influencing sabellid population growth on commercial farms. In order to better understand sabellid nutrition, the feeding behaviour of the sabellid in response to suspended solids of various particle size classes from both abalone diets were investigated. Both the effect of abalone diet and stocking density and their effects on sabellid morphometrics were quantified. The proximate composition and particle size distribution of suspended-solids present within kelp and Abfeed abalone raceways were also quantified. Results indicate that sabellid feeding follows no structured behavioural pattern for both abalone diets. The quantity of suspended solids in kelp raceways was greater than in Abfeed raceways (p<0.02) while there was no difference in particulate level between three abalone stocking densities (p>0.05). Protein (p<0.0001) and energy (p<0.0.1) levels of suspended solids were significantly greater in raceways dedicated to feeding Abfeed. The infestation level (number of tubes/centimetre on the grwoing edge) was significantly higher (p<0.001) in Abfeed-fed abalone. The morphometrics of the sabellids indicated that sabellids from Abfeed-fed abalone were larger in various body measurements: length (p<0.00001); neck width (p<0.001); base width (p<0.001); and surface area (p<0.001). This study suggests that abalone diet has an important influence on the infestation level and size of sabellids and that this is likely to be due to the fragmentation and leaching of nutrients from the artificially enriched commercial abalone diet.
- Full Text:
An investigation into the neuroprotective properties of curcumin
- Authors: Daniel, Sheril
- Date: 2003
- Subjects: Turmeric -- Therapeutic use , Nervous system -- Degeneration -- Prevention
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3753 , http://hdl.handle.net/10962/d1003231 , Turmeric -- Therapeutic use , Nervous system -- Degeneration -- Prevention
- Description: An increasing number of studies show that nutritional antioxidants such as vitamin E and polyphenols are capable of blocking neuronal death in vitro and may have therapeutic properties in animal models of neurodegenerative diseases including Alzheimer’s and Parkinson’s diseases. In the present study, the neuroprotective ability of one such polyphenolic antioxidant, curcumin, was investigated. Curcumin is the yellow curry spice derived from turmeric, and is widely used as a dietary component and herbal medicine in India. Most neurological disorders are postulated to have an oxidative or excitototoxic basis. Thus the effects of curcumin on oxidative stress in the rat brain were investigated. Curcumin, administered to the rat in vivo and in vitro, was able to exert protective effects on oxidative damage in the brain, induced by cyanide, a mitochondrial inhibitor. Curcumin also offered protection against quinolinic acid induced lipid peroxidation, and this protection was extended to lipid peroxidation induced by metals such as lead and cadmium in the rat brain. Experiments conducted on the pineal gland revealed an increased production of the neuroprotective hormone melatonin in presence of curcumin in vivo. The hippocampus is functionally related to vital behaviour and intellectual activities and is known to be a primary target for neuronal degeneration in the brains of patients with Alzheimer’s disease. Histological studies were undertaken to assess the effects of curcumin on lead induced toxicity on the rat hippocampus, the results of which show that curcumin affords significant protection to the hippocampus of the lead treated rats. This study also sought to elucidate possible mechanisms by which curcumin exerts its neuroprotective capabilities. Curcumin was found to inhibit the action of cyanide on the mitochondrial electron transport chain, one of the most common sources of free radicals. Electrochemical, UV/VIS and Infrared spectroscopy were used to characterise interactions between curcumin and the metals lead, cadmium, iron (II) and iron (III). Curcumin was shown to directly chelate these metals with the formation and isolation of two new curcumin complexes with lead, and one complex each with cadmium and iron (III). These results suggest chelation of toxic metals as a mechanism of neuroprotection afforded by curcumin. The need for neuroprotective agents is urgent considering the rapid rise in the elderly population and the proportionate increase in neurological disorders. The findings of this study indicate that curcumin, a well-established dietary antioxidant, is capable of playing a bigger role in neuroprotection, which needs to be further explored and exploited.
- Full Text:
- Authors: Daniel, Sheril
- Date: 2003
- Subjects: Turmeric -- Therapeutic use , Nervous system -- Degeneration -- Prevention
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3753 , http://hdl.handle.net/10962/d1003231 , Turmeric -- Therapeutic use , Nervous system -- Degeneration -- Prevention
- Description: An increasing number of studies show that nutritional antioxidants such as vitamin E and polyphenols are capable of blocking neuronal death in vitro and may have therapeutic properties in animal models of neurodegenerative diseases including Alzheimer’s and Parkinson’s diseases. In the present study, the neuroprotective ability of one such polyphenolic antioxidant, curcumin, was investigated. Curcumin is the yellow curry spice derived from turmeric, and is widely used as a dietary component and herbal medicine in India. Most neurological disorders are postulated to have an oxidative or excitototoxic basis. Thus the effects of curcumin on oxidative stress in the rat brain were investigated. Curcumin, administered to the rat in vivo and in vitro, was able to exert protective effects on oxidative damage in the brain, induced by cyanide, a mitochondrial inhibitor. Curcumin also offered protection against quinolinic acid induced lipid peroxidation, and this protection was extended to lipid peroxidation induced by metals such as lead and cadmium in the rat brain. Experiments conducted on the pineal gland revealed an increased production of the neuroprotective hormone melatonin in presence of curcumin in vivo. The hippocampus is functionally related to vital behaviour and intellectual activities and is known to be a primary target for neuronal degeneration in the brains of patients with Alzheimer’s disease. Histological studies were undertaken to assess the effects of curcumin on lead induced toxicity on the rat hippocampus, the results of which show that curcumin affords significant protection to the hippocampus of the lead treated rats. This study also sought to elucidate possible mechanisms by which curcumin exerts its neuroprotective capabilities. Curcumin was found to inhibit the action of cyanide on the mitochondrial electron transport chain, one of the most common sources of free radicals. Electrochemical, UV/VIS and Infrared spectroscopy were used to characterise interactions between curcumin and the metals lead, cadmium, iron (II) and iron (III). Curcumin was shown to directly chelate these metals with the formation and isolation of two new curcumin complexes with lead, and one complex each with cadmium and iron (III). These results suggest chelation of toxic metals as a mechanism of neuroprotection afforded by curcumin. The need for neuroprotective agents is urgent considering the rapid rise in the elderly population and the proportionate increase in neurological disorders. The findings of this study indicate that curcumin, a well-established dietary antioxidant, is capable of playing a bigger role in neuroprotection, which needs to be further explored and exploited.
- Full Text:
An investigation of knowledge management implementation strategies
- Authors: Sunassee, Nakkiran N
- Date: 2003
- Subjects: Knowledge management , Strategic planning , Automobile industry and trade -- South Africa -- Management
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:1141 , http://hdl.handle.net/10962/d1002770 , Knowledge management , Strategic planning , Automobile industry and trade -- South Africa -- Management
- Description: The world is experiencing an era which has been termed the “knowledge age” or the “knowledge economy”. In this new context, knowledge is the primary commodity, and knowledge flows are regarded as the most important factors in the economy. The management of knowledge flows within organisations has become a crucial activity because many of the activities of organisations and the broader economic and social life today are knowledge-driven. In recent years, this managerial activity has become known as Knowledge Management. Equally important are the associated Knowledge Management implementation strategies. Driven by a need expressed by the South African Motor Vehicle Industry for a knowledge management implementation strategy tailored to their needs, this research aimed to develop a knowledge management implementation strategy suited to the needs of this industry. Following an extensive literature survey and a study of the target industry using Duffy’s Knowledge Benchmarking Questionnaire, a model of knowledge management implementation is proposed that is suited to the needs of the South African Motor Vehicle Industry. The model consists of three main interlinked components: Knowledge Management of the Organisation, People, and Infrastructure and Processes. Furthermore, the model recommends a holistic approach to managing knowledge. The critical success factors of the model were tested by means of a survey of industry opinions that validated certain aspects of the model and motivated for changes in others. Additionally, despite the focus of the model on the target industry, it is considered sufficiently appropriate for use by other organisations.
- Full Text:
- Authors: Sunassee, Nakkiran N
- Date: 2003
- Subjects: Knowledge management , Strategic planning , Automobile industry and trade -- South Africa -- Management
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:1141 , http://hdl.handle.net/10962/d1002770 , Knowledge management , Strategic planning , Automobile industry and trade -- South Africa -- Management
- Description: The world is experiencing an era which has been termed the “knowledge age” or the “knowledge economy”. In this new context, knowledge is the primary commodity, and knowledge flows are regarded as the most important factors in the economy. The management of knowledge flows within organisations has become a crucial activity because many of the activities of organisations and the broader economic and social life today are knowledge-driven. In recent years, this managerial activity has become known as Knowledge Management. Equally important are the associated Knowledge Management implementation strategies. Driven by a need expressed by the South African Motor Vehicle Industry for a knowledge management implementation strategy tailored to their needs, this research aimed to develop a knowledge management implementation strategy suited to the needs of this industry. Following an extensive literature survey and a study of the target industry using Duffy’s Knowledge Benchmarking Questionnaire, a model of knowledge management implementation is proposed that is suited to the needs of the South African Motor Vehicle Industry. The model consists of three main interlinked components: Knowledge Management of the Organisation, People, and Infrastructure and Processes. Furthermore, the model recommends a holistic approach to managing knowledge. The critical success factors of the model were tested by means of a survey of industry opinions that validated certain aspects of the model and motivated for changes in others. Additionally, despite the focus of the model on the target industry, it is considered sufficiently appropriate for use by other organisations.
- Full Text:
Applied studies of some Southern African blowflies (Diptera: Calliphoridae) of forensic importance
- Authors: Lunt, Nicola
- Date: 2003
- Subjects: Diptera -- South Africa , Blowflies -- South Africa , Forensic entomology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5803 , http://hdl.handle.net/10962/d1006202 , Diptera -- South Africa , Blowflies -- South Africa , Forensic entomology
- Description: Three major aspects of blowfly (Diptera: Calliphoridae) research were the focus of this study. Firstly, the phylogenetic relationships of 40 oestroid species from a variety of geographical localities were investigated using Cytochrome Oxidase b subunit I (COl) gene sequences. Maximum parsimony (MP) and Jukes-Cantor neighbor-joining (NJ) analyses both extracted a paraphyletic Calliphoridae, with the Calliphorinae-Luciliinae clade being sister to the Sarcophagidae. Short branch lengths within Chrysomya indicate a recent rapid radiation of this genus. Phormia and Protophormia either formed a sister clade to Chrysomya, or were embedded in this genus. Tree topologies were comparable between MP and NJ trees, but the positions of some genera were ambiguous. Secondly, developmental parameters and behaviour were investigated for four southern African species of forensically important blowflies viz. Chrysomya chloropyga, C. putoria, C. megacephala and Lucilia sericata, and ad hoc observations were made for Calliphora croceipalpis, Chrysomya marginalis and the predatory C. albiceps. Choice of oviposition substrate differed between species, mirroring substrate preferences in the field. Sexual dimorphism and dwarfism within a cohort complicated ageing maggots using size, but the use of developmental events (e.g. ecdysis) allowed ages to be determined unambiguously. Separate species status was supported for the previously synonymised C. chloropyga and C. putoria, by differences in maggot behaviour, larval growth rates and temperature optima. The proportion of total development time assigned to each larval instar and pupariation was variable among temperatures, but similar between congeneric species. Thirdly, since a negative linear relationship was found to occur between the developmental constant (K) and developmental zero (D₀) for both Calliphoridae and Sarcophagidae, the potential for predicting physiological parameters of unstudied taxa was investigated. Species and genera of Palaearctic origin generally had high K's and low D₀'s, and the reverse was true for the tropical taxa. It was found that both K and D₀ can be estimated for "unknown" taxa using the Felsenstein's Independent Contrasts (FIC) method of PDTree (Garland et al. 200 I), provided that branch lengths are relatively short and the phylogenetic position of the estimated taxon is unambiguous.
- Full Text:
- Authors: Lunt, Nicola
- Date: 2003
- Subjects: Diptera -- South Africa , Blowflies -- South Africa , Forensic entomology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5803 , http://hdl.handle.net/10962/d1006202 , Diptera -- South Africa , Blowflies -- South Africa , Forensic entomology
- Description: Three major aspects of blowfly (Diptera: Calliphoridae) research were the focus of this study. Firstly, the phylogenetic relationships of 40 oestroid species from a variety of geographical localities were investigated using Cytochrome Oxidase b subunit I (COl) gene sequences. Maximum parsimony (MP) and Jukes-Cantor neighbor-joining (NJ) analyses both extracted a paraphyletic Calliphoridae, with the Calliphorinae-Luciliinae clade being sister to the Sarcophagidae. Short branch lengths within Chrysomya indicate a recent rapid radiation of this genus. Phormia and Protophormia either formed a sister clade to Chrysomya, or were embedded in this genus. Tree topologies were comparable between MP and NJ trees, but the positions of some genera were ambiguous. Secondly, developmental parameters and behaviour were investigated for four southern African species of forensically important blowflies viz. Chrysomya chloropyga, C. putoria, C. megacephala and Lucilia sericata, and ad hoc observations were made for Calliphora croceipalpis, Chrysomya marginalis and the predatory C. albiceps. Choice of oviposition substrate differed between species, mirroring substrate preferences in the field. Sexual dimorphism and dwarfism within a cohort complicated ageing maggots using size, but the use of developmental events (e.g. ecdysis) allowed ages to be determined unambiguously. Separate species status was supported for the previously synonymised C. chloropyga and C. putoria, by differences in maggot behaviour, larval growth rates and temperature optima. The proportion of total development time assigned to each larval instar and pupariation was variable among temperatures, but similar between congeneric species. Thirdly, since a negative linear relationship was found to occur between the developmental constant (K) and developmental zero (D₀) for both Calliphoridae and Sarcophagidae, the potential for predicting physiological parameters of unstudied taxa was investigated. Species and genera of Palaearctic origin generally had high K's and low D₀'s, and the reverse was true for the tropical taxa. It was found that both K and D₀ can be estimated for "unknown" taxa using the Felsenstein's Independent Contrasts (FIC) method of PDTree (Garland et al. 200 I), provided that branch lengths are relatively short and the phylogenetic position of the estimated taxon is unambiguous.
- Full Text:
Bioaccumulation and histopathology of copper in Oreochromis mossambicus
- Authors: Naigaga, Irene
- Date: 2003
- Subjects: Mozambique tilapia , Copper , Marine toxins , Fishes -- Effect of water pollution on , Water -- Pollution -- Environmental aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5234 , http://hdl.handle.net/10962/d1005077 , Mozambique tilapia , Copper , Marine toxins , Fishes -- Effect of water pollution on , Water -- Pollution -- Environmental aspects
- Description: Cu is one of the most toxic elements that affect fish populations when the fish are exposed to concentrations exceeding their tolerance. To investigate the effects of elementary Cu on aspects of bioconcentration, histology and behaviour, O. mossambicus were exposed to 0 and 0.75 ± 0.20 mg/l of Cu for 96 hours (short-term study), and 0, 0.11 ± 0.02, 0.29 ± 0.02, and 0.47 ± 0.04 mg/l of Cu for 64 days (longterm study) under controlled conditions in the laboratory. For the long-term study fish were sampled for gills, liver, and kidney Cu accumulation analysis after 1, 32 and 64 days of exposure and after 1, 2, 4, 16, 32, and 64 days for gills, liver and spleen histology analysis. Cu accumulation was concentration-duration dependent with the highest accumulation capacity in the liver. A multifactor linear model was developed for the relationship between exposure dose, exposure duration and Cu accumulation in the organs with the liver model: Log L = 3.35 + 0.85W + 0.31T (r² = 0.892) giving a better fit than the gills: G = −35.09 + 10.58W + 17.58T (r² = 0.632). Where L = Cu accumulation values in the liver, G = Cu accumulation values in the gills (both in μg/g dry mass); W = exposure dose in water (mg/l); and T = exposure time (days). Using this model Cu accumulation in organs can be estimated when exposure concentration and duration is known. This model should be tested under different conditions to determine the potential of the model in monitoring Cu toxicity in the environment. Lesions were observed in the liver, gills and spleen in all Cu treatments at all exposure concentration and exposure durations. However, the incidence and the degree of alteration was related to the concentration of Cu and duration of exposure. The sequential appearance of lesions in the order of, hepatic vacuolar degeneration, fatty degeneration and necrosis indicated a gradual increase in liver damage with larger duration of exposure time and increasing Cu concentration. The initial lesions in the gills were manifested as hypertrophy and hyperplasia of the gill epithelium causing increase in the thickness of the secondary lamellae, mucous cell hypertrophy and proliferation, mucous hypersecretion, proliferation of eosinophilic granule cells and hyperplasia of interlamellar cells. With increase in exposure time, necrosis of the eosinophilic granule cells, lamellar oedema, epithelial desquamation and increase in severity of lamellar hyperplasia were observed. These lesions indicated an initial defence mechanism of the fish against Cu toxicity followed by advanced histological changes that were related to Cu concentration and duration of exposure. Changes in the spleen were haemosiderosis, increase in the white pulp and macrophage centres, reduction in the red pulp, and necrosis suggesting that fish exposed to environmentally relevant levels of Cu may be histopathologically altered leading to anaemia and immunosuppression. Regression analysis was used to quantify the relationship between the total activity of the fish, and duration of exposure. There was a gradual decline in fish activity related to Cu concentration and duration of exposure before introducing food into the tanks. There was a constant activity after introducing food in the tanks at the control and 0.11 ± 0.02 mg/l Cu exposure levels irrespective of exposure time. Analysis of covariance (ANCOVA) was used to test for the difference in slopes between treatments. There was no significant difference (p > 0.05) between slopes of the control and 0.11 ± 0.02 mg/l Cu, and between 0.29 ± 0.02 and 47 ± 0.04 mg/l Cu before and after introducing food in the tanks. The slopes of both the control and 0.11 ± 0.02 mg/l Cu were significantly different from those of 0.29 ± 0.02 and 0.47 ± 0.04 mg/l Cu (p < 0.05). There were significant differences in the mean opercular movements per minute between treatments (p < 0.05). There was hyperventilation at 0.11 ± 0.02 mg/l Cu i.e. 87 ± 18 opercular movements per minute (mean ± standard deviation) and hypoventilation at 0.29 ± 0.02 and 0.47 ± 0.04 mg/l Cu i.e. 37 ± 34 and 13 ± 6 opercular movements per minute compared to the control. Hypo- and hyperventilation were related to the lesser and greater gill damage, respectively. In conclusion Cu accumulation and effects on histology of the liver, gills and were related to the concentration of Cu in the water and duration of exposure showing a gradual increase in incidence and intensity with larger duration of exposure time and increasing Cu concentration. The fish were initially able to homeostatically regulate and detoxify Cu. However, as the exposure continued, the homeostatic mechanism appears to have failed to cope with the increasing metal burden causing advanced histological changes.
- Full Text:
- Authors: Naigaga, Irene
- Date: 2003
- Subjects: Mozambique tilapia , Copper , Marine toxins , Fishes -- Effect of water pollution on , Water -- Pollution -- Environmental aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5234 , http://hdl.handle.net/10962/d1005077 , Mozambique tilapia , Copper , Marine toxins , Fishes -- Effect of water pollution on , Water -- Pollution -- Environmental aspects
- Description: Cu is one of the most toxic elements that affect fish populations when the fish are exposed to concentrations exceeding their tolerance. To investigate the effects of elementary Cu on aspects of bioconcentration, histology and behaviour, O. mossambicus were exposed to 0 and 0.75 ± 0.20 mg/l of Cu for 96 hours (short-term study), and 0, 0.11 ± 0.02, 0.29 ± 0.02, and 0.47 ± 0.04 mg/l of Cu for 64 days (longterm study) under controlled conditions in the laboratory. For the long-term study fish were sampled for gills, liver, and kidney Cu accumulation analysis after 1, 32 and 64 days of exposure and after 1, 2, 4, 16, 32, and 64 days for gills, liver and spleen histology analysis. Cu accumulation was concentration-duration dependent with the highest accumulation capacity in the liver. A multifactor linear model was developed for the relationship between exposure dose, exposure duration and Cu accumulation in the organs with the liver model: Log L = 3.35 + 0.85W + 0.31T (r² = 0.892) giving a better fit than the gills: G = −35.09 + 10.58W + 17.58T (r² = 0.632). Where L = Cu accumulation values in the liver, G = Cu accumulation values in the gills (both in μg/g dry mass); W = exposure dose in water (mg/l); and T = exposure time (days). Using this model Cu accumulation in organs can be estimated when exposure concentration and duration is known. This model should be tested under different conditions to determine the potential of the model in monitoring Cu toxicity in the environment. Lesions were observed in the liver, gills and spleen in all Cu treatments at all exposure concentration and exposure durations. However, the incidence and the degree of alteration was related to the concentration of Cu and duration of exposure. The sequential appearance of lesions in the order of, hepatic vacuolar degeneration, fatty degeneration and necrosis indicated a gradual increase in liver damage with larger duration of exposure time and increasing Cu concentration. The initial lesions in the gills were manifested as hypertrophy and hyperplasia of the gill epithelium causing increase in the thickness of the secondary lamellae, mucous cell hypertrophy and proliferation, mucous hypersecretion, proliferation of eosinophilic granule cells and hyperplasia of interlamellar cells. With increase in exposure time, necrosis of the eosinophilic granule cells, lamellar oedema, epithelial desquamation and increase in severity of lamellar hyperplasia were observed. These lesions indicated an initial defence mechanism of the fish against Cu toxicity followed by advanced histological changes that were related to Cu concentration and duration of exposure. Changes in the spleen were haemosiderosis, increase in the white pulp and macrophage centres, reduction in the red pulp, and necrosis suggesting that fish exposed to environmentally relevant levels of Cu may be histopathologically altered leading to anaemia and immunosuppression. Regression analysis was used to quantify the relationship between the total activity of the fish, and duration of exposure. There was a gradual decline in fish activity related to Cu concentration and duration of exposure before introducing food into the tanks. There was a constant activity after introducing food in the tanks at the control and 0.11 ± 0.02 mg/l Cu exposure levels irrespective of exposure time. Analysis of covariance (ANCOVA) was used to test for the difference in slopes between treatments. There was no significant difference (p > 0.05) between slopes of the control and 0.11 ± 0.02 mg/l Cu, and between 0.29 ± 0.02 and 47 ± 0.04 mg/l Cu before and after introducing food in the tanks. The slopes of both the control and 0.11 ± 0.02 mg/l Cu were significantly different from those of 0.29 ± 0.02 and 0.47 ± 0.04 mg/l Cu (p < 0.05). There were significant differences in the mean opercular movements per minute between treatments (p < 0.05). There was hyperventilation at 0.11 ± 0.02 mg/l Cu i.e. 87 ± 18 opercular movements per minute (mean ± standard deviation) and hypoventilation at 0.29 ± 0.02 and 0.47 ± 0.04 mg/l Cu i.e. 37 ± 34 and 13 ± 6 opercular movements per minute compared to the control. Hypo- and hyperventilation were related to the lesser and greater gill damage, respectively. In conclusion Cu accumulation and effects on histology of the liver, gills and were related to the concentration of Cu in the water and duration of exposure showing a gradual increase in incidence and intensity with larger duration of exposure time and increasing Cu concentration. The fish were initially able to homeostatically regulate and detoxify Cu. However, as the exposure continued, the homeostatic mechanism appears to have failed to cope with the increasing metal burden causing advanced histological changes.
- Full Text:
Biology and demography of the spotted grunter Pomadasys commersonnii (Haemulidae) in South African waters
- Authors: Webb, Garth Anthony
- Date: 2003
- Subjects: Grunts (Fishes) -- South Africa , Grunts (Fishes) -- South Africa -- Ecology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5201 , http://hdl.handle.net/10962/d1003727 , Grunts (Fishes) -- South Africa , Grunts (Fishes) -- South Africa -- Ecology
- Description: The spotted grunter, Pomadasys commersonnii (Haemulidae), is an Indian Ocean coastal species, extending from India to False Bay but is absent from the central Indian Ocean islands. Its taste and texture has made it one of the most important line fish species and is caught by recreational and subsistence fisherman along the entire east coast of South Africa. Because of its inshore distribution, reduced catch rates and estuarine dependence the species was de-commercialised in 1992. Since then it has been investigated as a candidate species for mariculture. All previous work on the biology of the species was undertaken on fish collected in KwaZulu-Natal in the mid 1970's. All other information has been incidental and formed part of other ecological studies. All management plans for this species have been based on these data. To develop a more comprehensive management plan that incorporates the entire population of spotted grunter, it was deemed necessary to reassess the biology (including feeding biology, age and growth and reproductive biology) as well as the demography of the population throughout its distributional range in South African waters. Analysis of the diet of spotted grunter, collected in estuaries, indicates that crustaceans form the bulk of the prey selected. Amphipods, mysids and estuarine brachyura predominate the diet of fish < 300 mm TL. At 200 mm TL fish start to prey on anomurans, which are extracted from their burrows using the 'blowing' feeding mechanism. Anomurans, in particular Upogebia africana and Callianassa krausii, become the preferred prey of fish larger than 300 mm TL. The high degree of diet flexibility that spotted grunter exhibit means that the composition and abundance of the macrobenthos of a particular environment will dictate the diet of the species. Otolith growth zones were found to be deposited annually with the opaque zones being deposited during the austral summer (November - February). The optical definition of annual otolith growth rings differed significantly between geographic regions (namely: Western Cape, South Eastern Cape and KwaZulu-Natal). Growth (sex combined) offish in the Western Cape were best described by the specialised 3 parameter Von Bertalanffy with a relative error structure in the form : Lt = 753(1- e⁻°·¹⁵⁴ ⁽t +¹·⁶¹⁵⁾) while growth in the South Eastern Cape was best described by using the Schnute model with an absolute error structure in the form: Lt=177°·⁴¹⁶ + (676.2⁻¹·²⁶⁶ - 169.2⁻¹²⁶⁶)[1-e°·⁴¹⁶⁽t-t₁⁾ / 1-e°·⁴¹⁶⁽t₂-t₁⁾]¹/¹·²⁶⁶ whereas growth was best described in KwaZulu-Natal using the specialised 3 parameter Von Bertalanffy with a relative error structure in the form: Lt = 839(1-e°·¹⁷⁽t⁺°·⁴⁹⁾) In the South Eastern Cape, length at 50% maturity was found to be 305 mm TL for males. Since females with ripe & running or spent gonads were not found in the South Eastern Cape and since histological evidence suggests that females in the South Eastern Cape have spawned, it appears that spawning does not occur in the South Eastern Cape. These results suggest that adults are resident in the estuaries of the Western and South Eastern Cape and undertake the spring/summer, northward spawning migration to KwaZulu-Natal. After joining the resident spawner stock in KwaZulu-Natal and spawning in the offshore environment of KwaZulu-Natal, adults soon return to the southern regions of their distributional range. Juveniles recruit into KwaZulu-Natal estuaries at a length of 25 - 35 mm TL. A proportion of the eggs and larvae are transported southwards along the periphery of the western boundary Agulhas Current where juveniles (25 - 30 mm TL) recruit into the estuaries as far south as the Swartvlei estuary in the Western Cape. There is evidence to suggest that the fish, which occur in the Western Cape estuaries, have migrated there once they have attained sexual maturity further east.
- Full Text:
- Authors: Webb, Garth Anthony
- Date: 2003
- Subjects: Grunts (Fishes) -- South Africa , Grunts (Fishes) -- South Africa -- Ecology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5201 , http://hdl.handle.net/10962/d1003727 , Grunts (Fishes) -- South Africa , Grunts (Fishes) -- South Africa -- Ecology
- Description: The spotted grunter, Pomadasys commersonnii (Haemulidae), is an Indian Ocean coastal species, extending from India to False Bay but is absent from the central Indian Ocean islands. Its taste and texture has made it one of the most important line fish species and is caught by recreational and subsistence fisherman along the entire east coast of South Africa. Because of its inshore distribution, reduced catch rates and estuarine dependence the species was de-commercialised in 1992. Since then it has been investigated as a candidate species for mariculture. All previous work on the biology of the species was undertaken on fish collected in KwaZulu-Natal in the mid 1970's. All other information has been incidental and formed part of other ecological studies. All management plans for this species have been based on these data. To develop a more comprehensive management plan that incorporates the entire population of spotted grunter, it was deemed necessary to reassess the biology (including feeding biology, age and growth and reproductive biology) as well as the demography of the population throughout its distributional range in South African waters. Analysis of the diet of spotted grunter, collected in estuaries, indicates that crustaceans form the bulk of the prey selected. Amphipods, mysids and estuarine brachyura predominate the diet of fish < 300 mm TL. At 200 mm TL fish start to prey on anomurans, which are extracted from their burrows using the 'blowing' feeding mechanism. Anomurans, in particular Upogebia africana and Callianassa krausii, become the preferred prey of fish larger than 300 mm TL. The high degree of diet flexibility that spotted grunter exhibit means that the composition and abundance of the macrobenthos of a particular environment will dictate the diet of the species. Otolith growth zones were found to be deposited annually with the opaque zones being deposited during the austral summer (November - February). The optical definition of annual otolith growth rings differed significantly between geographic regions (namely: Western Cape, South Eastern Cape and KwaZulu-Natal). Growth (sex combined) offish in the Western Cape were best described by the specialised 3 parameter Von Bertalanffy with a relative error structure in the form : Lt = 753(1- e⁻°·¹⁵⁴ ⁽t +¹·⁶¹⁵⁾) while growth in the South Eastern Cape was best described by using the Schnute model with an absolute error structure in the form: Lt=177°·⁴¹⁶ + (676.2⁻¹·²⁶⁶ - 169.2⁻¹²⁶⁶)[1-e°·⁴¹⁶⁽t-t₁⁾ / 1-e°·⁴¹⁶⁽t₂-t₁⁾]¹/¹·²⁶⁶ whereas growth was best described in KwaZulu-Natal using the specialised 3 parameter Von Bertalanffy with a relative error structure in the form: Lt = 839(1-e°·¹⁷⁽t⁺°·⁴⁹⁾) In the South Eastern Cape, length at 50% maturity was found to be 305 mm TL for males. Since females with ripe & running or spent gonads were not found in the South Eastern Cape and since histological evidence suggests that females in the South Eastern Cape have spawned, it appears that spawning does not occur in the South Eastern Cape. These results suggest that adults are resident in the estuaries of the Western and South Eastern Cape and undertake the spring/summer, northward spawning migration to KwaZulu-Natal. After joining the resident spawner stock in KwaZulu-Natal and spawning in the offshore environment of KwaZulu-Natal, adults soon return to the southern regions of their distributional range. Juveniles recruit into KwaZulu-Natal estuaries at a length of 25 - 35 mm TL. A proportion of the eggs and larvae are transported southwards along the periphery of the western boundary Agulhas Current where juveniles (25 - 30 mm TL) recruit into the estuaries as far south as the Swartvlei estuary in the Western Cape. There is evidence to suggest that the fish, which occur in the Western Cape estuaries, have migrated there once they have attained sexual maturity further east.
- Full Text:
Botanical inventory and phenology in relation to foraging behaviour of the Cape honeybees (Apis Mellifera Capensis) at a site in the Eastern Cape, South Africa
- Authors: Merti, Admassu Addi
- Date: 2003
- Subjects: Fynbos -- South Africa -- Eastern Cape , Honeybee -- South Africa -- Eastern Cape , Honey plants -- South Africa -- Eastern Cape , Honeybee -- South Africa -- Eastern Cape -- Food , Plant ecology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4214 , http://hdl.handle.net/10962/d1003783 , Fynbos -- South Africa -- Eastern Cape , Honeybee -- South Africa -- Eastern Cape , Honey plants -- South Africa -- Eastern Cape , Honeybee -- South Africa -- Eastern Cape -- Food , Plant ecology -- South Africa -- Eastern Cape
- Description: From an apicultural point of view the Cape fynbos is under-utilised and our knowledge of its utilization by the Cape honeybees is incomplete. The key aim of this study was to test the hypothesis that the Cape honeybees utilize the fynbos species as the preferred source of nectar and pollen. Subsidiary aims included distinguishing vegetation communities in the area, identifying pollen and nectar sources, the relationship between brood population and seasonal pollen collection patterns, examining the effect of meteorological factors on pollen collection. The study site was on Rivendell Farm within the Eastern Cape Albany district: an area of high species richness. A checklist of vascular plant species was produced revealing 97 families, 271 genera and 448 species. A classification by two-way indicator species (TWINSPAN) recognized seven vegetation communities: Forest, Bush clumps, Acacia savanna, Grassland, Grassy fynbos, Fynbos and Shrubland. Direct field observations of the foraging of Cape honeybees identified 54 nectar and pollen source plant species. Honeybee pollen loads trapped from four colonies of hives identified 37 pollen source plants of which Metalasia muricata, Eucalyptus grandis, Eucalyptus camaldulensis, Erica chamissonis, Helichrysum odoratissimum, Helichrysum anomalum, Crassula cultrata and Acacia longifolia were the predominant pollen source plants. It was also found that 60% of pollen yield derived from fynbos vegetation. The pollen source plants came from both Cape endemic and from nonendemic species. Thus we reject the hypothesis that Cape honeybees selectively forage fynbos species as a preferred source of pollen and nectar. The examination of the effect of temperature, wind-speed and temperature on pollen collection activity of honeybees revealed that: a temperature range of between 14°C to 26°C was optimal for pollen collection; wind speeds of up to 4m/s were conducive for pollen collection; relative humidity was found to have no significant influence on pollen collection. Pollen collection and brood rearing patterns are positively correlated with flowering intensities, but we found in our Eastern Cape study site that brood rearing was not limited to the spring flowering season but did extend to the end of summer. In order to determine the available nectar yield of common plant species hourly secretion of nectar volumes was measured for 24 hours to determine the variation of available nectar during different times of the day. In all nectar producing species the nectar volume was high in the early morning and declined as the day progressed. We found that the volume of available nectar was affected by prevailing temperature and humidity around the flowers.
- Full Text:
- Authors: Merti, Admassu Addi
- Date: 2003
- Subjects: Fynbos -- South Africa -- Eastern Cape , Honeybee -- South Africa -- Eastern Cape , Honey plants -- South Africa -- Eastern Cape , Honeybee -- South Africa -- Eastern Cape -- Food , Plant ecology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4214 , http://hdl.handle.net/10962/d1003783 , Fynbos -- South Africa -- Eastern Cape , Honeybee -- South Africa -- Eastern Cape , Honey plants -- South Africa -- Eastern Cape , Honeybee -- South Africa -- Eastern Cape -- Food , Plant ecology -- South Africa -- Eastern Cape
- Description: From an apicultural point of view the Cape fynbos is under-utilised and our knowledge of its utilization by the Cape honeybees is incomplete. The key aim of this study was to test the hypothesis that the Cape honeybees utilize the fynbos species as the preferred source of nectar and pollen. Subsidiary aims included distinguishing vegetation communities in the area, identifying pollen and nectar sources, the relationship between brood population and seasonal pollen collection patterns, examining the effect of meteorological factors on pollen collection. The study site was on Rivendell Farm within the Eastern Cape Albany district: an area of high species richness. A checklist of vascular plant species was produced revealing 97 families, 271 genera and 448 species. A classification by two-way indicator species (TWINSPAN) recognized seven vegetation communities: Forest, Bush clumps, Acacia savanna, Grassland, Grassy fynbos, Fynbos and Shrubland. Direct field observations of the foraging of Cape honeybees identified 54 nectar and pollen source plant species. Honeybee pollen loads trapped from four colonies of hives identified 37 pollen source plants of which Metalasia muricata, Eucalyptus grandis, Eucalyptus camaldulensis, Erica chamissonis, Helichrysum odoratissimum, Helichrysum anomalum, Crassula cultrata and Acacia longifolia were the predominant pollen source plants. It was also found that 60% of pollen yield derived from fynbos vegetation. The pollen source plants came from both Cape endemic and from nonendemic species. Thus we reject the hypothesis that Cape honeybees selectively forage fynbos species as a preferred source of pollen and nectar. The examination of the effect of temperature, wind-speed and temperature on pollen collection activity of honeybees revealed that: a temperature range of between 14°C to 26°C was optimal for pollen collection; wind speeds of up to 4m/s were conducive for pollen collection; relative humidity was found to have no significant influence on pollen collection. Pollen collection and brood rearing patterns are positively correlated with flowering intensities, but we found in our Eastern Cape study site that brood rearing was not limited to the spring flowering season but did extend to the end of summer. In order to determine the available nectar yield of common plant species hourly secretion of nectar volumes was measured for 24 hours to determine the variation of available nectar during different times of the day. In all nectar producing species the nectar volume was high in the early morning and declined as the day progressed. We found that the volume of available nectar was affected by prevailing temperature and humidity around the flowers.
- Full Text:
Cimetidine as a free radical scavenger
- Authors: Lambat, Zaynab Yusuf
- Date: 2003
- Subjects: Cimetidine , Cimetidine -- Physiological effect , Cimetidine -- Therapeutic use , Alzheimer's disease -- Treatment , Cancer -- Treatment , Free radicals (Chemistry) -- Physiological effect
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3766 , http://hdl.handle.net/10962/d1003244 , Cimetidine , Cimetidine -- Physiological effect , Cimetidine -- Therapeutic use , Alzheimer's disease -- Treatment , Cancer -- Treatment , Free radicals (Chemistry) -- Physiological effect
- Description: The present study was undertaken to determine the effects and possible mechanism of action of cimetidine in cancer and Alzheimer’s disease (AD). Throughout this study emphasis is placed on free radical levels since the magnitude of the relationship between diseases and the levels of free radicals vary from one disease to another. Studies were carried out to examine the effect of cimetidine on free radical levels using superoxide formation and lipid peroxidation as indicators of free radical levels. The experiments revealed that addition of cimetidine, especially in high concentrations (0.5 and 1.0 x10-6 M) significantly inhibited WHCO6 cancer cell growth rather than cancer cell growth, as no normal control was available. Free radical formation as well as hydroxyl radical formation were reduced in the deoxyribose assay. In addition, cimetidine exhibits properties of binding to metals such as copper and iron. To maintain consistency in the experiments, a WHCO6 (Wits Human Carcinoma of the Oesophagus) cell line was used to investigate the effect of cimetidine in cancer. Neurodegeneration was induced in the rat brain using neurotoxins such as cyanide to investigate the relationship between cimetidine in AD. A decrease in cancer cell growth was accompanied by a concomitant decrease in the levels of free radicals and lipid peroxidation, suggesting that the growth-inhibitory effects of cimetidine on WHCO6 cancer cells in vitro may be due to free radical scavenging properties. This proposal was further strengthened by determination of free radical levels in the rat brain. After treatment with neurotoxins to induce neurodegeneration, the levels of free radicals in the rat brain suggest that addition of cimetidine reduces free radical levels in the rat brain in a dosedependent manner. Further experiments were done in an attempt to uncover the underlying mechanism by which cimetidine exhibits free radical scavenging properties. Metal binding studies were done using electrochemical, HPLC and UV/Vis studies. The results show that cimetidine binds iron and copper. These metals have been implicated in free radical production via the Fenton reaction. By binding with cimetidine the metals become unavailable to produce free radicals and hence cimetidine indirectly reduces the formation of free radicals. The final experiment was the determination of cimetidine as a hydroxyl radical scavenger in the deoxyribose assay. Cimetidine was shown to act as a potent hydroxyl radical scavenger, thereby confirming its activity as a free radical scavenger. In addition, cimetidine protects against damage to the deoxyribose sugar, a component of DNA. Whilst there are many theories that explain the therapeutic role of cimetidine in degenerative disease, the actual mechanism of the role of cimetidine is emphasized as a free radical scavenger. Regardless of the mechanism of action, cimetidine does inhibit tumour growth according to this study and also reduce free radical levels in neurodegeneration, which suggests a role for cimetidine as a possible additive in treatment of patients with such disease states. These findings have important clinical implications, and needs to be investigated further.
- Full Text:
- Authors: Lambat, Zaynab Yusuf
- Date: 2003
- Subjects: Cimetidine , Cimetidine -- Physiological effect , Cimetidine -- Therapeutic use , Alzheimer's disease -- Treatment , Cancer -- Treatment , Free radicals (Chemistry) -- Physiological effect
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3766 , http://hdl.handle.net/10962/d1003244 , Cimetidine , Cimetidine -- Physiological effect , Cimetidine -- Therapeutic use , Alzheimer's disease -- Treatment , Cancer -- Treatment , Free radicals (Chemistry) -- Physiological effect
- Description: The present study was undertaken to determine the effects and possible mechanism of action of cimetidine in cancer and Alzheimer’s disease (AD). Throughout this study emphasis is placed on free radical levels since the magnitude of the relationship between diseases and the levels of free radicals vary from one disease to another. Studies were carried out to examine the effect of cimetidine on free radical levels using superoxide formation and lipid peroxidation as indicators of free radical levels. The experiments revealed that addition of cimetidine, especially in high concentrations (0.5 and 1.0 x10-6 M) significantly inhibited WHCO6 cancer cell growth rather than cancer cell growth, as no normal control was available. Free radical formation as well as hydroxyl radical formation were reduced in the deoxyribose assay. In addition, cimetidine exhibits properties of binding to metals such as copper and iron. To maintain consistency in the experiments, a WHCO6 (Wits Human Carcinoma of the Oesophagus) cell line was used to investigate the effect of cimetidine in cancer. Neurodegeneration was induced in the rat brain using neurotoxins such as cyanide to investigate the relationship between cimetidine in AD. A decrease in cancer cell growth was accompanied by a concomitant decrease in the levels of free radicals and lipid peroxidation, suggesting that the growth-inhibitory effects of cimetidine on WHCO6 cancer cells in vitro may be due to free radical scavenging properties. This proposal was further strengthened by determination of free radical levels in the rat brain. After treatment with neurotoxins to induce neurodegeneration, the levels of free radicals in the rat brain suggest that addition of cimetidine reduces free radical levels in the rat brain in a dosedependent manner. Further experiments were done in an attempt to uncover the underlying mechanism by which cimetidine exhibits free radical scavenging properties. Metal binding studies were done using electrochemical, HPLC and UV/Vis studies. The results show that cimetidine binds iron and copper. These metals have been implicated in free radical production via the Fenton reaction. By binding with cimetidine the metals become unavailable to produce free radicals and hence cimetidine indirectly reduces the formation of free radicals. The final experiment was the determination of cimetidine as a hydroxyl radical scavenger in the deoxyribose assay. Cimetidine was shown to act as a potent hydroxyl radical scavenger, thereby confirming its activity as a free radical scavenger. In addition, cimetidine protects against damage to the deoxyribose sugar, a component of DNA. Whilst there are many theories that explain the therapeutic role of cimetidine in degenerative disease, the actual mechanism of the role of cimetidine is emphasized as a free radical scavenger. Regardless of the mechanism of action, cimetidine does inhibit tumour growth according to this study and also reduce free radical levels in neurodegeneration, which suggests a role for cimetidine as a possible additive in treatment of patients with such disease states. These findings have important clinical implications, and needs to be investigated further.
- Full Text:
Confirmation of a slow symplasmic loading and unloading pathway in barley (Hordeum Vulgare L.) source and sink leaves
- Authors: Buwa, Lisa Valencia
- Date: 2003
- Subjects: Phloem , Plant translocation , Barley -- Metabolism
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4253 , http://hdl.handle.net/10962/d1007804
- Description: Visualization of the transport pathway in barley (Hordeum vulgare) leaves was carried out using a combination of aniline blue and a symplasmically transported fluorochrome, 5,6 carboxyfluorescein (5,6-CF). When applied to a source leaf, basipetal movement of 5,6-CF was observed after 3 h and the fluorochrome front was observed about 3-4cm away from the point of application. The fluorochrome was taken up into the symplasm of the mesophyll and was loaded into the bundle sheath cells and then subsequently the vascular parenchyma and finally into the sieve tubes. In sink leaves, acropetal movement was observed after 3 h and the fluorochrome had moved approximately 3 cm away from the point of application. Unloading of 5,6-CF occurred from all classes of longitudinal veins. Studies on solute retrieval showed that 5,6 CF-diacetate was transferred to xylem parenchyma where it was metabolized. 5,6-CF was then transferred from the xylem parenchyma to the vascular parenchyma cells, and it would appear that thick-walled sieve tubes were the first to show 5,6-CF labeling. Counterstaining with aniline blue demonstrates the presence of plasmodesmata and this suggests a potential symplasmic pathway from the mesophyll to the sieve tubes. Application of 5,6 CF-diacetate revealed a slow symplasmic pathway, which involved transfer of 5,6-CF, which was effected via plasmodesma.
- Full Text:
- Authors: Buwa, Lisa Valencia
- Date: 2003
- Subjects: Phloem , Plant translocation , Barley -- Metabolism
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4253 , http://hdl.handle.net/10962/d1007804
- Description: Visualization of the transport pathway in barley (Hordeum vulgare) leaves was carried out using a combination of aniline blue and a symplasmically transported fluorochrome, 5,6 carboxyfluorescein (5,6-CF). When applied to a source leaf, basipetal movement of 5,6-CF was observed after 3 h and the fluorochrome front was observed about 3-4cm away from the point of application. The fluorochrome was taken up into the symplasm of the mesophyll and was loaded into the bundle sheath cells and then subsequently the vascular parenchyma and finally into the sieve tubes. In sink leaves, acropetal movement was observed after 3 h and the fluorochrome had moved approximately 3 cm away from the point of application. Unloading of 5,6-CF occurred from all classes of longitudinal veins. Studies on solute retrieval showed that 5,6 CF-diacetate was transferred to xylem parenchyma where it was metabolized. 5,6-CF was then transferred from the xylem parenchyma to the vascular parenchyma cells, and it would appear that thick-walled sieve tubes were the first to show 5,6-CF labeling. Counterstaining with aniline blue demonstrates the presence of plasmodesmata and this suggests a potential symplasmic pathway from the mesophyll to the sieve tubes. Application of 5,6 CF-diacetate revealed a slow symplasmic pathway, which involved transfer of 5,6-CF, which was effected via plasmodesma.
- Full Text:
Cyclodepsipeptides from a Kenyan marine cyanobacterium
- Authors: Dzeha, Thomas Mwambire
- Date: 2003
- Subjects: Cyanobacteria , Stereochemistry , Natural products -- Kenya
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4303 , http://hdl.handle.net/10962/d1004961 , Cyanobacteria , Stereochemistry , Natural products -- Kenya
- Description: An examination of an organic extract of the cyanobacterium Lyngbya majuscula collected from Wasini Island off the southern Kenyan coast led to the isolation of the known cyclodepsipeptide antanapeptin A (7), recently isolated from a Madagascan collection of L. majuscula, and a new bioactive cyclodepsipeptide, homodolastatin 16 (42). Although L. majuscula is a common, pantropical cyanobacterium this study represents the first investigation of the natural product chemistry of a Kenyan population of L. majuscula. The structures of the two cyclodepsipeptides were determined from 2D NMR and mass spectrometry data. The L- stereochemistry of the proline, valine, and N-methylphenylalanine amino acids in 7 and the L – proline configuration in 42, was confirmed by Marfey’s HPLC method. Chiral GC was used to determine the absolute stereochemistry of the hydroxyisovaleric acid moiety in 7 and 42, the lactate residue in 42 and tentatively propose an L-stereochemistry for the Nmethylisoleucine amino acid in 42. Homodolastatin 16, a higher homologue of the potential anti-cancer agent, dolastatin 16, exhibited moderate activity against two oesophageal cancer cell lines.
- Full Text:
- Authors: Dzeha, Thomas Mwambire
- Date: 2003
- Subjects: Cyanobacteria , Stereochemistry , Natural products -- Kenya
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4303 , http://hdl.handle.net/10962/d1004961 , Cyanobacteria , Stereochemistry , Natural products -- Kenya
- Description: An examination of an organic extract of the cyanobacterium Lyngbya majuscula collected from Wasini Island off the southern Kenyan coast led to the isolation of the known cyclodepsipeptide antanapeptin A (7), recently isolated from a Madagascan collection of L. majuscula, and a new bioactive cyclodepsipeptide, homodolastatin 16 (42). Although L. majuscula is a common, pantropical cyanobacterium this study represents the first investigation of the natural product chemistry of a Kenyan population of L. majuscula. The structures of the two cyclodepsipeptides were determined from 2D NMR and mass spectrometry data. The L- stereochemistry of the proline, valine, and N-methylphenylalanine amino acids in 7 and the L – proline configuration in 42, was confirmed by Marfey’s HPLC method. Chiral GC was used to determine the absolute stereochemistry of the hydroxyisovaleric acid moiety in 7 and 42, the lactate residue in 42 and tentatively propose an L-stereochemistry for the Nmethylisoleucine amino acid in 42. Homodolastatin 16, a higher homologue of the potential anti-cancer agent, dolastatin 16, exhibited moderate activity against two oesophageal cancer cell lines.
- Full Text:
Effects of small-scale water movement on the settlement and growth rates of the brown mussel Perna perna, on the south-east coast of South Africa
- Authors: Mathagu, Tendamudzimu Titus
- Date: 2003
- Subjects: Mussels -- South Africa , Perna -- South Africa , Perna -- Growth
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5682 , http://hdl.handle.net/10962/d1005368 , Mussels -- South Africa , Perna -- South Africa , Perna -- Growth
- Description: The effects of small scale (cm) water movement on the settlement and growth rates of the brown mussel Perna perna were investigated on the south-east coast of South Africa (33°28′S, 27°10′E). L-shaped metal baffles attached to the substratum decreased the erosion rates of cement balls and it was concluded that the baffles decreased the water flow rate around cement balls. These L-shaped baffles were then used to decrease water flow rates around mussel patches and pot-scouring pads used as artificial substrata for the settlement of P.perna larvae. Anova indicated that settlement rate varied by date and site while decreased water flow rate significantly increased larval settlement (p<0.05), only on the site and day that had the overall highest number of settlers. Mussels in the low zone had significantly higher growth rates than those in the high zone. Decreased water flow rate significantly increased mussel growth rate in the lower zone (Anova, p<0.05), while it did not have a significant effect on the mussel in the high zone. Thus water flow manipulation increased growth rates in the zone, which already had high growth rate. It was concluded that small-scale (cm) water flow patterns have an effect on both Perna perna settlement and growth rates, but only under specific conditions. Larval settlement rate was significantly increased by water flow manipulation on the site and day that had the highest number of settlers. Growth rates were significantly increased by decreased water flow rate only in the low zone, where growth rates are the highest. Although water flow was manipulated in both zones its effect in the high zone was insignificant (Anova) compared to other factors affecting growth rates at this tidal level.
- Full Text:
- Authors: Mathagu, Tendamudzimu Titus
- Date: 2003
- Subjects: Mussels -- South Africa , Perna -- South Africa , Perna -- Growth
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5682 , http://hdl.handle.net/10962/d1005368 , Mussels -- South Africa , Perna -- South Africa , Perna -- Growth
- Description: The effects of small scale (cm) water movement on the settlement and growth rates of the brown mussel Perna perna were investigated on the south-east coast of South Africa (33°28′S, 27°10′E). L-shaped metal baffles attached to the substratum decreased the erosion rates of cement balls and it was concluded that the baffles decreased the water flow rate around cement balls. These L-shaped baffles were then used to decrease water flow rates around mussel patches and pot-scouring pads used as artificial substrata for the settlement of P.perna larvae. Anova indicated that settlement rate varied by date and site while decreased water flow rate significantly increased larval settlement (p<0.05), only on the site and day that had the overall highest number of settlers. Mussels in the low zone had significantly higher growth rates than those in the high zone. Decreased water flow rate significantly increased mussel growth rate in the lower zone (Anova, p<0.05), while it did not have a significant effect on the mussel in the high zone. Thus water flow manipulation increased growth rates in the zone, which already had high growth rate. It was concluded that small-scale (cm) water flow patterns have an effect on both Perna perna settlement and growth rates, but only under specific conditions. Larval settlement rate was significantly increased by water flow manipulation on the site and day that had the highest number of settlers. Growth rates were significantly increased by decreased water flow rate only in the low zone, where growth rates are the highest. Although water flow was manipulated in both zones its effect in the high zone was insignificant (Anova) compared to other factors affecting growth rates at this tidal level.
- Full Text:
Finite element modelling of a magma chamber surrounded by country-rock, with particular reference to the groundwater flow in sections of different permeability
- Authors: Remsing, Carmen
- Date: 2003 , 2013-05-23
- Subjects: Magmas , Groundwater flow
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5508 , http://hdl.handle.net/10962/d1007537 , Magmas , Groundwater flow
- Description: This thesis presents results of two-dimensional finite element modelling of a magma chamber surrounded by country-rock containing a section of high permeability. The high permeability section in the country-rock simulates structure that is predominant in controlling the groundwater convection pattern and resulting mineral deposits. The models have analogies in nature: for instance the gold mines in the Massif Central of France, the Pogo mine in Alaska and the Pilgrim's Rest gold field in South Africa. This is a complicated coupled system involving fluid flow and heat transfer under extreme conditions. The magma in the chamber convects and as it cools the heat liberated causes convection in the groundwater contained in the surrounding country-rock. This convection in turn affects the rate of liberation of heat from the magma. The software used for the modelling, FLOTRAN, is the computational fluid dynamics component of the commercial ANSYS package. The results obtained describe in detail the flow pattern in the magma chamber, the country-rock and high permeability section thereof. During the cooling of the magma chamber the groundwater convects more vigorously in the high permeability section than elsewhere, and a convection cell is seen forming within this region. This provides a mechanism for hydrothermal formation of valuable mineral deposits in the structure near a magma chamber. It is found that the relationship between the velocity of the flow in the cell and the temperature of the magma chamber is well represented by a first order linear differential equation, providing a simple understanding of this process, , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
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- Authors: Remsing, Carmen
- Date: 2003 , 2013-05-23
- Subjects: Magmas , Groundwater flow
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5508 , http://hdl.handle.net/10962/d1007537 , Magmas , Groundwater flow
- Description: This thesis presents results of two-dimensional finite element modelling of a magma chamber surrounded by country-rock containing a section of high permeability. The high permeability section in the country-rock simulates structure that is predominant in controlling the groundwater convection pattern and resulting mineral deposits. The models have analogies in nature: for instance the gold mines in the Massif Central of France, the Pogo mine in Alaska and the Pilgrim's Rest gold field in South Africa. This is a complicated coupled system involving fluid flow and heat transfer under extreme conditions. The magma in the chamber convects and as it cools the heat liberated causes convection in the groundwater contained in the surrounding country-rock. This convection in turn affects the rate of liberation of heat from the magma. The software used for the modelling, FLOTRAN, is the computational fluid dynamics component of the commercial ANSYS package. The results obtained describe in detail the flow pattern in the magma chamber, the country-rock and high permeability section thereof. During the cooling of the magma chamber the groundwater convects more vigorously in the high permeability section than elsewhere, and a convection cell is seen forming within this region. This provides a mechanism for hydrothermal formation of valuable mineral deposits in the structure near a magma chamber. It is found that the relationship between the velocity of the flow in the cell and the temperature of the magma chamber is well represented by a first order linear differential equation, providing a simple understanding of this process, , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
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Investigation of the comparative cost-effectiveness of different strategies for the management of multidrug-resistant tuberculosis
- Authors: Rockcliffe, Nicole
- Date: 2003
- Subjects: Tuberculosis , Multidrug resistance , Tuberculosis -- Treatment
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3788 , http://hdl.handle.net/10962/d1003266 , Tuberculosis , Multidrug resistance , Tuberculosis -- Treatment
- Description: The tuberculosis epidemic is escalating in South Africa as well as globally. This escalation is exacerbated by the increasing prevalence of multidrug-resistant tuberculosis (MDRTB), which is defined by the World Health Organisation (WHO) as resistance of Mycobacteria to at least isoniazid and rifampicin. Multi-drug resistant tuberculosis is estimated to occur in 1-2% of newly diagnosed tuberculosis (TB) patients and in 4-8% of previously treated patients. MDRTB is both difficult and expensive to treat, costing up to 126 times that of drug-sensitive TB. Resource constrained countries such as South Africa often lack both the money and the infrastructure to treat this disease. The aim of this project was to determine whether the performance of a systematic review with subsequent economic modelling could influence the decision making process for policy makers. Data was gathered and an economic evaluation of MDRTB treatment was performed from the perspective of the South African Department of Health. Three treatment alternatives were identified: a protocol regimen of second line anti-tuberculosis agents, as recommended in the South African guidelines for MDRTB, an appropriate regimen designed for each patient according to the results of culture and drug susceptibility tests, and non-drug management. A decision-analysis model using DATA 3.0 by Treeage® was developed to estimate the costs of each alternative. Outcomes were measured in terms of cost alone as well as the ‘number of cases cured’ and the number of ‘years of life saved’ for patients dying, being cured or failing treatment. Drug, hospital and laboratory costs incurred using each alternative were included in the analysis. A sensitivity analysis was performed on all variables in order to identify threshold values that would change the outcome of the evaluation. Results of the decision analysis indicate that the individualised regimen was both the cheaper and more cost-effective regimen of the two active treatment options, and was estimated to cost R50 661 per case cured and R2 070 per year of life saved. The protocol regimen was estimated to cost R73 609 per case cured and R2 741 per year of life saved. The outcome of the decision analysis was sensitive to changes in some of the variables used to model the disease, particularly the daily cost of drugs, the length of time spent in hospital and the length of treatment received by those patients dying or failing treatment. This modelling exercise highlighted significant deficiencies in the quality of evidence on MDRTB management available to policy makers. Pragmatic choices based on operational and other logistic concerns may need to be reviewed when further information becomes available. A case can be made for the establishment of a national database of costing and efficacy information to guide future policy revisions of the South African MDRTB treatment programme, which is resource intensive and of only moderate efficacy. However, due to the widely disparate range of studies on which this evaluation was based, the outcome of the study may not be credible. In this case, the use of a systematic review with subsequent economic modelling could not validly influence policy-makers to change the decision that they made on the basis of drug availability.
- Full Text:
- Authors: Rockcliffe, Nicole
- Date: 2003
- Subjects: Tuberculosis , Multidrug resistance , Tuberculosis -- Treatment
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3788 , http://hdl.handle.net/10962/d1003266 , Tuberculosis , Multidrug resistance , Tuberculosis -- Treatment
- Description: The tuberculosis epidemic is escalating in South Africa as well as globally. This escalation is exacerbated by the increasing prevalence of multidrug-resistant tuberculosis (MDRTB), which is defined by the World Health Organisation (WHO) as resistance of Mycobacteria to at least isoniazid and rifampicin. Multi-drug resistant tuberculosis is estimated to occur in 1-2% of newly diagnosed tuberculosis (TB) patients and in 4-8% of previously treated patients. MDRTB is both difficult and expensive to treat, costing up to 126 times that of drug-sensitive TB. Resource constrained countries such as South Africa often lack both the money and the infrastructure to treat this disease. The aim of this project was to determine whether the performance of a systematic review with subsequent economic modelling could influence the decision making process for policy makers. Data was gathered and an economic evaluation of MDRTB treatment was performed from the perspective of the South African Department of Health. Three treatment alternatives were identified: a protocol regimen of second line anti-tuberculosis agents, as recommended in the South African guidelines for MDRTB, an appropriate regimen designed for each patient according to the results of culture and drug susceptibility tests, and non-drug management. A decision-analysis model using DATA 3.0 by Treeage® was developed to estimate the costs of each alternative. Outcomes were measured in terms of cost alone as well as the ‘number of cases cured’ and the number of ‘years of life saved’ for patients dying, being cured or failing treatment. Drug, hospital and laboratory costs incurred using each alternative were included in the analysis. A sensitivity analysis was performed on all variables in order to identify threshold values that would change the outcome of the evaluation. Results of the decision analysis indicate that the individualised regimen was both the cheaper and more cost-effective regimen of the two active treatment options, and was estimated to cost R50 661 per case cured and R2 070 per year of life saved. The protocol regimen was estimated to cost R73 609 per case cured and R2 741 per year of life saved. The outcome of the decision analysis was sensitive to changes in some of the variables used to model the disease, particularly the daily cost of drugs, the length of time spent in hospital and the length of treatment received by those patients dying or failing treatment. This modelling exercise highlighted significant deficiencies in the quality of evidence on MDRTB management available to policy makers. Pragmatic choices based on operational and other logistic concerns may need to be reviewed when further information becomes available. A case can be made for the establishment of a national database of costing and efficacy information to guide future policy revisions of the South African MDRTB treatment programme, which is resource intensive and of only moderate efficacy. However, due to the widely disparate range of studies on which this evaluation was based, the outcome of the study may not be credible. In this case, the use of a systematic review with subsequent economic modelling could not validly influence policy-makers to change the decision that they made on the basis of drug availability.
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