An investigation into the extended use of mobile phone technology in the cellular industry in Port Elizabeth
- Authors: Inman, Michael Christopher
- Date: 2004
- Subjects: Cell phone services industry -- South Africa -- Port Elizabeth , Cell phones , Cell phone systems
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10923 , http://hdl.handle.net/10948/247 , Cell phone services industry -- South Africa -- Port Elizabeth , Cell phones , Cell phone systems
- Description: While experiencing exponential growth worldwide, Information and Communication Technologies (ICTs) are key factors in business today. The ubiquitous nature of the mobile phone in South Africa and the use of wireless technologies facilitate business in areas where current fixed infrastructure is limited or non-existent, thereby aiding in the development of rural areas. This descriptive study determines what factors will extend the use of mobile phone technology in business. The literature indicates business advantage can be gained from incorporating technology into a strategic framework but in so doing must consider the complexities of the technology and its lifecycle. Currently, e-mail and Internet access would be the most likely applications of data use via Global System for Mobile Communications (GSM). As a technology-based study the methodology used e-mail to deliver a survey targeting staff from the three cellular network operators in Port Elizabeth. The mobile industry and Port Elizabeth were both considered good indicators of technology use and the progression of infrastructure throughout South Africa. The findings indicate that although most mobile phones have the features required by users, the current use of associated data applications is low. Email is the most appropriate application and expected to be significant in 2006. The technology is of strategic importance and can be applied to make idle time productive. Successful use depends on the removal of barriers. In the case of e-mail and Internet access; the most significant of these is the complexity of the initial set-up. Despite the complexity of technology evolution and the challenges of effective business application, substantial gains can be still be realised. Currently, technology is best deployed to meet specialised needs. The expected introduction of third generation technologies herald’s higher data transfer rates, providing the opportunity for more generalised application in business in the immediate future.
- Full Text:
- Date Issued: 2004
- Authors: Inman, Michael Christopher
- Date: 2004
- Subjects: Cell phone services industry -- South Africa -- Port Elizabeth , Cell phones , Cell phone systems
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10923 , http://hdl.handle.net/10948/247 , Cell phone services industry -- South Africa -- Port Elizabeth , Cell phones , Cell phone systems
- Description: While experiencing exponential growth worldwide, Information and Communication Technologies (ICTs) are key factors in business today. The ubiquitous nature of the mobile phone in South Africa and the use of wireless technologies facilitate business in areas where current fixed infrastructure is limited or non-existent, thereby aiding in the development of rural areas. This descriptive study determines what factors will extend the use of mobile phone technology in business. The literature indicates business advantage can be gained from incorporating technology into a strategic framework but in so doing must consider the complexities of the technology and its lifecycle. Currently, e-mail and Internet access would be the most likely applications of data use via Global System for Mobile Communications (GSM). As a technology-based study the methodology used e-mail to deliver a survey targeting staff from the three cellular network operators in Port Elizabeth. The mobile industry and Port Elizabeth were both considered good indicators of technology use and the progression of infrastructure throughout South Africa. The findings indicate that although most mobile phones have the features required by users, the current use of associated data applications is low. Email is the most appropriate application and expected to be significant in 2006. The technology is of strategic importance and can be applied to make idle time productive. Successful use depends on the removal of barriers. In the case of e-mail and Internet access; the most significant of these is the complexity of the initial set-up. Despite the complexity of technology evolution and the challenges of effective business application, substantial gains can be still be realised. Currently, technology is best deployed to meet specialised needs. The expected introduction of third generation technologies herald’s higher data transfer rates, providing the opportunity for more generalised application in business in the immediate future.
- Full Text:
- Date Issued: 2004
An investigation into the impact of the use of an integrated learning system on mathematics standard grade paper 2 marks of grade 12 learners of one high school in the Nelson Mandela Metropolitan area
- Barnard, Stefanus van Rooyen
- Authors: Barnard, Stefanus van Rooyen
- Date: 2004
- Subjects: Mathematics -- Research -- South Africa -- Port Elizabeth , Mathematics -- Examinations -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Doctoral , DEd
- Identifier: vital:10780 , http://hdl.handle.net/10948/248 , Mathematics -- Research -- South Africa -- Port Elizabeth , Mathematics -- Examinations -- South Africa -- Port Elizabeth
- Description: The aim of this study was an exploration of the relationship between the use of an Integrated Learning System (ILS), entitled Master Maths, as a supplement to traditional mathematics instruction, and mathematics achievement as measured by the Paper 2 marks of the National Mathematics Examinations for standard grade learners in grade 12. The use of technology in education has increased over the past decade. One way of integrating technology into instructional programmes has been through the use of Integrated Learning Systems (ILSs). The review of the literature traces the history of computer-assisted instruction as conducted on ILSs. The review of recent research studies focuses on the impact of ILSs on learner achievement in mathematics internationally and in the South African context. This study used quantitative and qualitative methods to research the impact of the Master Maths programme on mathematics achievement. Twenty-six learners of the 133 standard grade learners from one high school in New Brighton, Port Elizabeth were selected for each of the experimental and control groups. The experimental group worked on the Master Maths programme for twelve sessions of three hours each. The results of the quantitative analysis show that the intervention did not make a significant difference to the experimental group. The Master Maths programme led to only a 0.56% increase in the marks of the experimental group. The qualitative analysis drew a comparison between the modules of the Master Maths programme and the relevant examination questions in terms of content covered and cognitive levels. The researcher used Bloom’s Cognitive Taxonomy to evaluate the cognitive levels. The data show that it was easier for the learners to obtain higher marks in the module tests than in the examination questions. The data indicate that the module tests were easier than the examination questions in that the cognitive levels of the module tests were lower. The data confirm that there is a gap between the acquisition and evaluation of core skills tested by the modules used in the intervention and the wider knowledge and skills tested in the examination.
- Full Text:
- Date Issued: 2004
- Authors: Barnard, Stefanus van Rooyen
- Date: 2004
- Subjects: Mathematics -- Research -- South Africa -- Port Elizabeth , Mathematics -- Examinations -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Doctoral , DEd
- Identifier: vital:10780 , http://hdl.handle.net/10948/248 , Mathematics -- Research -- South Africa -- Port Elizabeth , Mathematics -- Examinations -- South Africa -- Port Elizabeth
- Description: The aim of this study was an exploration of the relationship between the use of an Integrated Learning System (ILS), entitled Master Maths, as a supplement to traditional mathematics instruction, and mathematics achievement as measured by the Paper 2 marks of the National Mathematics Examinations for standard grade learners in grade 12. The use of technology in education has increased over the past decade. One way of integrating technology into instructional programmes has been through the use of Integrated Learning Systems (ILSs). The review of the literature traces the history of computer-assisted instruction as conducted on ILSs. The review of recent research studies focuses on the impact of ILSs on learner achievement in mathematics internationally and in the South African context. This study used quantitative and qualitative methods to research the impact of the Master Maths programme on mathematics achievement. Twenty-six learners of the 133 standard grade learners from one high school in New Brighton, Port Elizabeth were selected for each of the experimental and control groups. The experimental group worked on the Master Maths programme for twelve sessions of three hours each. The results of the quantitative analysis show that the intervention did not make a significant difference to the experimental group. The Master Maths programme led to only a 0.56% increase in the marks of the experimental group. The qualitative analysis drew a comparison between the modules of the Master Maths programme and the relevant examination questions in terms of content covered and cognitive levels. The researcher used Bloom’s Cognitive Taxonomy to evaluate the cognitive levels. The data show that it was easier for the learners to obtain higher marks in the module tests than in the examination questions. The data indicate that the module tests were easier than the examination questions in that the cognitive levels of the module tests were lower. The data confirm that there is a gap between the acquisition and evaluation of core skills tested by the modules used in the intervention and the wider knowledge and skills tested in the examination.
- Full Text:
- Date Issued: 2004
An investigation into the neuroprotective effects of melatonin in a model of rotenone-induced neurodegeneration
- Authors: Kadanthode, Rubina John
- Date: 2004
- Subjects: Melatonin , Nervous system -- Degeneration -- Treatment , Rotenone
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3763 , http://hdl.handle.net/10962/d1003241 , Melatonin , Nervous system -- Degeneration -- Treatment , Rotenone
- Description: Parkinson’s disease, one of the most common neurodegenerative disorders associated with ageing, is characterised by abnormal and profound loss of nigrostriatal dopaminergic neurons. The cause of Parkinson’s disease is unknown, but epidemiological studies suggest an association with pesticides and other environmental toxins, and biochemical studies implicate oxidative damage and mitochondrial impairment, particularly at the level of complex I enzyme. Recently, rotenone, a commonly used organic pesticide and a classical inhibitor of mitochondrial complex I has been reported to reproduce the specific features of Parkinson’s disease in rodents. The mitochondrial respiratory chain is one of the most important sites of reactive oxygen species production under physiological conditions. Toxic free radicals have been implicated in a variety of neurodegenerative diseases as well as ageing itself. Melatonin, a secretory product of the pineal gland is a multifaceted free radical scavenger and natural antioxidant. In the present study, the neuroprotective effects of melatonin against the environmental neurotoxin, rotenone was investigated. Initial studies showed that inhibition of mitochondrial complex I enzyme by rotenone induced superoxide radical generation. Melatonin, administered to the rat in vivo and in vitro was able to offer neuroprotection by curtailing the production of superoxide radicals induced by rotenone. Mitochondria, being the major target of rotenone, the effects of melatonin were investigated at the mitochondrial level. Melatonin was able to increase the electron transport chain activity thus preventing the respiratory inhibition by rotenone. The pineal hormone also counteracted the action of rotenone on complex I enzyme. These results suggest melatonin’s ability to potentially limit the free radical generation and thereby modulate the mitochondrial functions. The detection and measurement of lipid peroxidation is the evidence most frequently cited to support the involvement of free radical reactions in toxicology and in human disease. Melatonin also offered significant protection in vivo and in vitro against rotenone induced lipid peroxidation. Since iron plays a major role in oxidative damage and in the progression of Parkinson’s disease, the effect of melatonin on both rotenone and iron induced lipid peroxidation was investigated, the results of which show that melatonin affords protection and this was suggested to be due to its interaction with the rotenone-iron complex that might have formed. Electrochemical studies were further used to characterise the interactions between melatonin, rotenone and iron (III). Melatonin was shown to bind with iron and thus reducing their toxicity. Histological studies were undertaken to assess the effects of melatonin on rotenone induced toxicity on the dopaminergic neurons in the rat brain. Rotenone treated brains showed extensive neuronal damage whereas with melatonin less damage was observed. Rotenone induces apoptosis via reactive oxygen species production and apoptotic cell death has been identified in PD brains. Furthermore, the apoptotic cell death was detected and quantified by the TUNEL staining. Rotenone treated sections showed signs of apoptosis whereas with melatonin, less apoptotic damage was observed. The findings of this study indicate that the neurohormone, melatonin may protect against rotenone-induced neurodegeneration. Since melatonin production falls substantially during ageing, the loss of this antioxidant is theorized to be instrumental in the degenerative processes associated with advanced age. Considering how devastating diseases such as Parkinson’s disease, are to a patient and the patient’s families, the discovery of protective agents are a matter of urgency. Further investigations using the pesticide model will help to determine the involvement of environmental exposure in the pathogenesis of human diseases as well as to test therapeutic strategies for the treatment of such diseases.
- Full Text:
- Date Issued: 2004
- Authors: Kadanthode, Rubina John
- Date: 2004
- Subjects: Melatonin , Nervous system -- Degeneration -- Treatment , Rotenone
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3763 , http://hdl.handle.net/10962/d1003241 , Melatonin , Nervous system -- Degeneration -- Treatment , Rotenone
- Description: Parkinson’s disease, one of the most common neurodegenerative disorders associated with ageing, is characterised by abnormal and profound loss of nigrostriatal dopaminergic neurons. The cause of Parkinson’s disease is unknown, but epidemiological studies suggest an association with pesticides and other environmental toxins, and biochemical studies implicate oxidative damage and mitochondrial impairment, particularly at the level of complex I enzyme. Recently, rotenone, a commonly used organic pesticide and a classical inhibitor of mitochondrial complex I has been reported to reproduce the specific features of Parkinson’s disease in rodents. The mitochondrial respiratory chain is one of the most important sites of reactive oxygen species production under physiological conditions. Toxic free radicals have been implicated in a variety of neurodegenerative diseases as well as ageing itself. Melatonin, a secretory product of the pineal gland is a multifaceted free radical scavenger and natural antioxidant. In the present study, the neuroprotective effects of melatonin against the environmental neurotoxin, rotenone was investigated. Initial studies showed that inhibition of mitochondrial complex I enzyme by rotenone induced superoxide radical generation. Melatonin, administered to the rat in vivo and in vitro was able to offer neuroprotection by curtailing the production of superoxide radicals induced by rotenone. Mitochondria, being the major target of rotenone, the effects of melatonin were investigated at the mitochondrial level. Melatonin was able to increase the electron transport chain activity thus preventing the respiratory inhibition by rotenone. The pineal hormone also counteracted the action of rotenone on complex I enzyme. These results suggest melatonin’s ability to potentially limit the free radical generation and thereby modulate the mitochondrial functions. The detection and measurement of lipid peroxidation is the evidence most frequently cited to support the involvement of free radical reactions in toxicology and in human disease. Melatonin also offered significant protection in vivo and in vitro against rotenone induced lipid peroxidation. Since iron plays a major role in oxidative damage and in the progression of Parkinson’s disease, the effect of melatonin on both rotenone and iron induced lipid peroxidation was investigated, the results of which show that melatonin affords protection and this was suggested to be due to its interaction with the rotenone-iron complex that might have formed. Electrochemical studies were further used to characterise the interactions between melatonin, rotenone and iron (III). Melatonin was shown to bind with iron and thus reducing their toxicity. Histological studies were undertaken to assess the effects of melatonin on rotenone induced toxicity on the dopaminergic neurons in the rat brain. Rotenone treated brains showed extensive neuronal damage whereas with melatonin less damage was observed. Rotenone induces apoptosis via reactive oxygen species production and apoptotic cell death has been identified in PD brains. Furthermore, the apoptotic cell death was detected and quantified by the TUNEL staining. Rotenone treated sections showed signs of apoptosis whereas with melatonin, less apoptotic damage was observed. The findings of this study indicate that the neurohormone, melatonin may protect against rotenone-induced neurodegeneration. Since melatonin production falls substantially during ageing, the loss of this antioxidant is theorized to be instrumental in the degenerative processes associated with advanced age. Considering how devastating diseases such as Parkinson’s disease, are to a patient and the patient’s families, the discovery of protective agents are a matter of urgency. Further investigations using the pesticide model will help to determine the involvement of environmental exposure in the pathogenesis of human diseases as well as to test therapeutic strategies for the treatment of such diseases.
- Full Text:
- Date Issued: 2004
An investigation into the promotional activities employed by the motorcycle businesses in the Nelson Mandela Metropolitan area
- Authors: Gouws, Aldo
- Date: 2004
- Subjects: Motorcycle industry -- South Africa -- Port Elizabeth , Motorcycles -- South Africa -- Port Elizabeth -- Marketing
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10860 , http://hdl.handle.net/10948/250 , Motorcycle industry -- South Africa -- Port Elizabeth , Motorcycles -- South Africa -- Port Elizabeth -- Marketing
- Description: The motorcycle industry is well established in South Africa, but has not nearly reached its full potential. This study investigates how this situation can be improved by enhancing the promotions of motorcycles in the Nelson Mandela Metropolitan Municipality (NMMM) area under investigation. Motorcycle businesses in this area currently compete with a disadvantage in the transportation market. Motor vehicles are assembled in this area whereas motorcycles are not, and this creates various work opportunities and brand loyalty for the motor industry. The Government also offers import rebates to motor vehicle exports. The cost, prejudicial attitudes and road safety of motorcycles contribute toward the difficulties faced by the motorcycle industry, which prevent the growth of this market. This market usually requires potential customers to have a passion for motorcycles to turn them into actual customers. To overcome these difficulties an investigation was conducted to determine which promotional activities the motorcycle businesses in the NMMM area employ. This study identifies the motorcycle businesses’ promotional shortcomings and makes suggestions for improvement. Promotional activities are vital since motorcycles are at a disadvantage when compared to motor vehicles in the NMMM area. One of the best ways the motorcycle businesses can successfully compete in the transportation market is to make use of promotions to make potential customers aware and enhance their support of the motorcycle businesses. For this reason the promotional variables (advertising, direct marketing, personal selling, sales promotions and public relations) should be exploited to its fullest to enhance potential customers’ support of motorcycles. This process can also create brand loyalty and equity, which will retain customers over the long term. Communication with a market is essential to achieve this and to inform and remind potential customers about the products available.
- Full Text:
- Date Issued: 2004
- Authors: Gouws, Aldo
- Date: 2004
- Subjects: Motorcycle industry -- South Africa -- Port Elizabeth , Motorcycles -- South Africa -- Port Elizabeth -- Marketing
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10860 , http://hdl.handle.net/10948/250 , Motorcycle industry -- South Africa -- Port Elizabeth , Motorcycles -- South Africa -- Port Elizabeth -- Marketing
- Description: The motorcycle industry is well established in South Africa, but has not nearly reached its full potential. This study investigates how this situation can be improved by enhancing the promotions of motorcycles in the Nelson Mandela Metropolitan Municipality (NMMM) area under investigation. Motorcycle businesses in this area currently compete with a disadvantage in the transportation market. Motor vehicles are assembled in this area whereas motorcycles are not, and this creates various work opportunities and brand loyalty for the motor industry. The Government also offers import rebates to motor vehicle exports. The cost, prejudicial attitudes and road safety of motorcycles contribute toward the difficulties faced by the motorcycle industry, which prevent the growth of this market. This market usually requires potential customers to have a passion for motorcycles to turn them into actual customers. To overcome these difficulties an investigation was conducted to determine which promotional activities the motorcycle businesses in the NMMM area employ. This study identifies the motorcycle businesses’ promotional shortcomings and makes suggestions for improvement. Promotional activities are vital since motorcycles are at a disadvantage when compared to motor vehicles in the NMMM area. One of the best ways the motorcycle businesses can successfully compete in the transportation market is to make use of promotions to make potential customers aware and enhance their support of the motorcycle businesses. For this reason the promotional variables (advertising, direct marketing, personal selling, sales promotions and public relations) should be exploited to its fullest to enhance potential customers’ support of motorcycles. This process can also create brand loyalty and equity, which will retain customers over the long term. Communication with a market is essential to achieve this and to inform and remind potential customers about the products available.
- Full Text:
- Date Issued: 2004
An investigation to establish whether the implementation of a structured total quality management system would add value to the South African Brewery, East London depot
- Authors: Herman, Dane
- Date: 2004
- Subjects: Total quality management , Brewing industry -- South Africa -- East London -- Quality control , Benchmarking (Management)
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10866 , http://hdl.handle.net/10948/254 , Total quality management , Brewing industry -- South Africa -- East London -- Quality control , Benchmarking (Management)
- Description: No matter how receptive or unreceptive an individual or a company is to the arrival of the age of technology, this phenomenon of change cannot be overlooked in the competitive global village. Companies must respond and change if they wish to survive into the next century. The results and testing of the hypotheses in chapters four and five clearly indicate that there is a need for a structured Total Quality Management (TQM) system in the East London Depot, of South African Breweries (SAB). The vision of the company states that they aim to be the “benchmark of South African industry and the brewing world”. The mission of the company states that they wish to provide their consumers with the finest quality malt beverages, brewed and marketed by world - class people in a socially responsible and innovative manner. Two of the core values of the company are: • Customer service and consumer focus • Innovation and quality (http://Beernet) Taking the afore mentioned as a guide line, it is evident that although there is a need for a structured TQM system at the East London Depot, there are key focus areas to concentrate on for the program to be successful. The main focus area will be to change the perception of the staff with regards to doing things right the first time. Customer service is very important and should be understood by all. A competent person should be tasked with the implementation of the system. This person should then manage the system and ensure that the staff training and maintenance of documented procedures are adhered to. A TQM committee must also be established in order to perform the necessary audits. As mentioned in chapter one, the aim of the depot management is to improve on it’s current national ranking. A structured TQM program will make a huge contribution towards achieving the desired result. This will result in better results and achievement of goals. With this in mind this paper aims to investigate the feasibility of establishing a T Q M system at the East London Depot of SAB.
- Full Text:
- Date Issued: 2004
- Authors: Herman, Dane
- Date: 2004
- Subjects: Total quality management , Brewing industry -- South Africa -- East London -- Quality control , Benchmarking (Management)
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10866 , http://hdl.handle.net/10948/254 , Total quality management , Brewing industry -- South Africa -- East London -- Quality control , Benchmarking (Management)
- Description: No matter how receptive or unreceptive an individual or a company is to the arrival of the age of technology, this phenomenon of change cannot be overlooked in the competitive global village. Companies must respond and change if they wish to survive into the next century. The results and testing of the hypotheses in chapters four and five clearly indicate that there is a need for a structured Total Quality Management (TQM) system in the East London Depot, of South African Breweries (SAB). The vision of the company states that they aim to be the “benchmark of South African industry and the brewing world”. The mission of the company states that they wish to provide their consumers with the finest quality malt beverages, brewed and marketed by world - class people in a socially responsible and innovative manner. Two of the core values of the company are: • Customer service and consumer focus • Innovation and quality (http://Beernet) Taking the afore mentioned as a guide line, it is evident that although there is a need for a structured TQM system at the East London Depot, there are key focus areas to concentrate on for the program to be successful. The main focus area will be to change the perception of the staff with regards to doing things right the first time. Customer service is very important and should be understood by all. A competent person should be tasked with the implementation of the system. This person should then manage the system and ensure that the staff training and maintenance of documented procedures are adhered to. A TQM committee must also be established in order to perform the necessary audits. As mentioned in chapter one, the aim of the depot management is to improve on it’s current national ranking. A structured TQM program will make a huge contribution towards achieving the desired result. This will result in better results and achievement of goals. With this in mind this paper aims to investigate the feasibility of establishing a T Q M system at the East London Depot of SAB.
- Full Text:
- Date Issued: 2004
An XML-Based Approach to the Generation and Testing of mLAN Sound Installation Configurations
- Foss, Richard, Laubscher, Ron, Fujimori, J I
- Authors: Foss, Richard , Laubscher, Ron , Fujimori, J I
- Date: 2004
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/427267 , vital:72426 , https://www.aes.org/e-lib/browse.cfm?elib=12787
- Description: An application, called the mLAN Installation Designer, has been developed that enables the user to graphically design and validate an mLAN sound installation. This application is built upon a model of mLAN systems that is defined by an Extensible Markup Language (XML) Schema, ensuring cross platform portability and future scalability. The XML Schema provides sufficient flexibility to form the basis for a standard effort to describe the configuration of IEEE 1394 based sound installation environments. The output from the mLAN Installation Designer application file is an XML document, consistent with the defined schema, which allows a configuration tool to configure the mLAN devices for automatic operation during deployment of the system.
- Full Text:
- Date Issued: 2004
- Authors: Foss, Richard , Laubscher, Ron , Fujimori, J I
- Date: 2004
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/427267 , vital:72426 , https://www.aes.org/e-lib/browse.cfm?elib=12787
- Description: An application, called the mLAN Installation Designer, has been developed that enables the user to graphically design and validate an mLAN sound installation. This application is built upon a model of mLAN systems that is defined by an Extensible Markup Language (XML) Schema, ensuring cross platform portability and future scalability. The XML Schema provides sufficient flexibility to form the basis for a standard effort to describe the configuration of IEEE 1394 based sound installation environments. The output from the mLAN Installation Designer application file is an XML document, consistent with the defined schema, which allows a configuration tool to configure the mLAN devices for automatic operation during deployment of the system.
- Full Text:
- Date Issued: 2004
Apartheid South Africa's foreign relations with African states, 1961-1994
- Authors: Pfister, Roger
- Date: 2004
- Subjects: Apartheid -- South Africa -- History -- 20th century Government, Resistance to -- South Africa Democracy -- South Africa South Africa -- Politics and government -- 20th century South Africa -- History -- 1961-1994 South Africa -- Foreign relations -- Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2863 , http://hdl.handle.net/10962/d1007632
- Description: This thesis examines South Africa's foreign relations, viewed from a South African perspective, with the black African countries beyond southern Africa from 1961 to 1994. These relations were determined by the conflict between Pretoria's apartheid ideology on the one hand, and African continental rejection of South Africa's race discrimination policies and its exclusion from the community of African states on the other. The documentary material used primarily stems from the Department of Foreign Affairs archive in Pretoria, supplemented by research conducted in other archives. Furthermore, we conducted interviews and correspondence, and consulted the relevant primary and secondary literature. Given the main source of information, we chose to make this work a case study in Diplomatic History. In consequence, and constituting the core of the study, Chapters 3 to 6 explore the interaction between South Africa and the black African states in a chronological order. At the same time, we draw on the analytical concepts from the academic disciplines of Political Science and its derivative, International Relations, to comprehend developments more fully. We discuss the significance of the approaches from these two disciplines in both the Introduction and Chapter 2. In particular, we emphasise that this study is about Pretoria's foreign policy, involving state and non-state actors, and we suggest that the unequal status between South Africa and the other African states constitutes an inherent factor in the relationship between them. The Conclusion examines the role of the state and non-state actors in determining Pretoria's foreign relations and the relevance of the structural imbalance between South Africa and the black African states in this context.
- Full Text:
- Date Issued: 2004
- Authors: Pfister, Roger
- Date: 2004
- Subjects: Apartheid -- South Africa -- History -- 20th century Government, Resistance to -- South Africa Democracy -- South Africa South Africa -- Politics and government -- 20th century South Africa -- History -- 1961-1994 South Africa -- Foreign relations -- Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2863 , http://hdl.handle.net/10962/d1007632
- Description: This thesis examines South Africa's foreign relations, viewed from a South African perspective, with the black African countries beyond southern Africa from 1961 to 1994. These relations were determined by the conflict between Pretoria's apartheid ideology on the one hand, and African continental rejection of South Africa's race discrimination policies and its exclusion from the community of African states on the other. The documentary material used primarily stems from the Department of Foreign Affairs archive in Pretoria, supplemented by research conducted in other archives. Furthermore, we conducted interviews and correspondence, and consulted the relevant primary and secondary literature. Given the main source of information, we chose to make this work a case study in Diplomatic History. In consequence, and constituting the core of the study, Chapters 3 to 6 explore the interaction between South Africa and the black African states in a chronological order. At the same time, we draw on the analytical concepts from the academic disciplines of Political Science and its derivative, International Relations, to comprehend developments more fully. We discuss the significance of the approaches from these two disciplines in both the Introduction and Chapter 2. In particular, we emphasise that this study is about Pretoria's foreign policy, involving state and non-state actors, and we suggest that the unequal status between South Africa and the other African states constitutes an inherent factor in the relationship between them. The Conclusion examines the role of the state and non-state actors in determining Pretoria's foreign relations and the relevance of the structural imbalance between South Africa and the black African states in this context.
- Full Text:
- Date Issued: 2004
Applied aquatic ecotoxicology sub-lethal methods, whole effluent testing and communication
- Palmer, Carolyn G, Muller, Nikite W J, Davies-Coleman, Heather D
- Authors: Palmer, Carolyn G , Muller, Nikite W J , Davies-Coleman, Heather D
- Date: 2004
- Subjects: To be catalogued
- Language: English
- Type: text , report
- Identifier: http://hdl.handle.net/10962/437785 , vital:73409 , ISBN 1-77005-252-6 , https://wrcwebsite.azurewebsites.net/wp-content/uploads/mdocs/1245-1-041.pdf
- Description: This report is the most recent in a series of WRC reports on the development of the capacity to undertake ecotoxicological research in South Africa. The development followed the following tines: • Recognition, as a result of the Kruger National Park Rivers Research Programme, that there were virtually no data on the water quality requirements of South African macroin vertebrates. • Development of the capacity to undertake experimental tolerance testing using riverine invertebrates in artificial stream systems. • Investigation of the salt tolerances, and whole effluent toxicity responses, of both standard toxicity test taxa and South African macroinverte-brates. • Development of both lethal and sub-lethal measures. • Application of re-search results to the development of methods for water quality within ecological Reserve determinations, and the implementation of the National Water Act (NWA) (No 36. of 1998) and National Water Resource Strategy (NWRS). The WRC is com-mitted to funding research that underpins the implementation of the NWA and the NWRS. Over the past 12 years it became clear that there would not be a rapid up-take of ecotoxicology research results in South Africa, and that it was important to place ecotoxicology in the wider context of water quality. From this recognition, the concept of Environmental Water Quality evolved.
- Full Text:
- Date Issued: 2004
- Authors: Palmer, Carolyn G , Muller, Nikite W J , Davies-Coleman, Heather D
- Date: 2004
- Subjects: To be catalogued
- Language: English
- Type: text , report
- Identifier: http://hdl.handle.net/10962/437785 , vital:73409 , ISBN 1-77005-252-6 , https://wrcwebsite.azurewebsites.net/wp-content/uploads/mdocs/1245-1-041.pdf
- Description: This report is the most recent in a series of WRC reports on the development of the capacity to undertake ecotoxicological research in South Africa. The development followed the following tines: • Recognition, as a result of the Kruger National Park Rivers Research Programme, that there were virtually no data on the water quality requirements of South African macroin vertebrates. • Development of the capacity to undertake experimental tolerance testing using riverine invertebrates in artificial stream systems. • Investigation of the salt tolerances, and whole effluent toxicity responses, of both standard toxicity test taxa and South African macroinverte-brates. • Development of both lethal and sub-lethal measures. • Application of re-search results to the development of methods for water quality within ecological Reserve determinations, and the implementation of the National Water Act (NWA) (No 36. of 1998) and National Water Resource Strategy (NWRS). The WRC is com-mitted to funding research that underpins the implementation of the NWA and the NWRS. Over the past 12 years it became clear that there would not be a rapid up-take of ecotoxicology research results in South Africa, and that it was important to place ecotoxicology in the wider context of water quality. From this recognition, the concept of Environmental Water Quality evolved.
- Full Text:
- Date Issued: 2004
Attitudes of isiXhosa-speaking students at the University of Fort Hare towards the use of isiXhosa as a language of learning and teaching (LOLT)
- Authors: Dalvit, Lorenzo
- Date: 2004
- Subjects: Native language and education -- South Africa -- Eastern Cape , Xhosa language -- Study and teaching (Higher) -- South Africa , English language -- Study and teaching (Higher) -- South Africa , Education, Bilingual , Language and education -- South Africa , Language policy -- South Africa , University of Fort Hare -- Students -- Attitudes
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2343 , http://hdl.handle.net/10962/d1002625 , Native language and education -- South Africa -- Eastern Cape , Xhosa language -- Study and teaching (Higher) -- South Africa , English language -- Study and teaching (Higher) -- South Africa , Education, Bilingual , Language and education -- South Africa , Language policy -- South Africa , University of Fort Hare -- Students -- Attitudes
- Description: The present work presents and discusses the results of a survey of a sample of isiXhosa-speaking students at the University of Fort Hare (Alice campus) and their attitudes towards the possible introduction of isiXhosa as a medium of instruction at this institution. More specifically, the research focuses on, among other things, the students’ attitudes towards English and isiXhosa and their opinions and beliefs about the introduction of dual-mediumship and its possible consequences. The survey methods used are a questionnaire survey as well as follow-up interviews, supplemented by on-campus observation. The results are first analysed as a whole, and then split into different categories according to a set of background variables (gender, year of study, subject studied etc). This analysis indicates that, while English is recognised as the dominant language in South Africa and, more specifically, in the domain of education, some categories of respondents acknowledge the usefulness of isiXhosa as an additional medium of instruction. This survey clearly shows that it makes little sense to present isiXhosa-speaking students with a rigid choice between the existing English-medium and a dual-medium (English and isiXhosa) policy. If dual-mediumship is ever to be implemented, respondents seem to consider the use of isiXhosa as a medium of instruction more appropriate in the first years of study, for selected subjects and in some domains within the academic context rather than others. This study is part of a growing set of surveys on the attitudes of university students towards the use of African languages in education, and can be fruitfully compared with similar research at other institutions. Moreover, the results of the present research can be used to inform future decisions regarding language policy at the University of Fort Hare.
- Full Text:
- Date Issued: 2004
- Authors: Dalvit, Lorenzo
- Date: 2004
- Subjects: Native language and education -- South Africa -- Eastern Cape , Xhosa language -- Study and teaching (Higher) -- South Africa , English language -- Study and teaching (Higher) -- South Africa , Education, Bilingual , Language and education -- South Africa , Language policy -- South Africa , University of Fort Hare -- Students -- Attitudes
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2343 , http://hdl.handle.net/10962/d1002625 , Native language and education -- South Africa -- Eastern Cape , Xhosa language -- Study and teaching (Higher) -- South Africa , English language -- Study and teaching (Higher) -- South Africa , Education, Bilingual , Language and education -- South Africa , Language policy -- South Africa , University of Fort Hare -- Students -- Attitudes
- Description: The present work presents and discusses the results of a survey of a sample of isiXhosa-speaking students at the University of Fort Hare (Alice campus) and their attitudes towards the possible introduction of isiXhosa as a medium of instruction at this institution. More specifically, the research focuses on, among other things, the students’ attitudes towards English and isiXhosa and their opinions and beliefs about the introduction of dual-mediumship and its possible consequences. The survey methods used are a questionnaire survey as well as follow-up interviews, supplemented by on-campus observation. The results are first analysed as a whole, and then split into different categories according to a set of background variables (gender, year of study, subject studied etc). This analysis indicates that, while English is recognised as the dominant language in South Africa and, more specifically, in the domain of education, some categories of respondents acknowledge the usefulness of isiXhosa as an additional medium of instruction. This survey clearly shows that it makes little sense to present isiXhosa-speaking students with a rigid choice between the existing English-medium and a dual-medium (English and isiXhosa) policy. If dual-mediumship is ever to be implemented, respondents seem to consider the use of isiXhosa as a medium of instruction more appropriate in the first years of study, for selected subjects and in some domains within the academic context rather than others. This study is part of a growing set of surveys on the attitudes of university students towards the use of African languages in education, and can be fruitfully compared with similar research at other institutions. Moreover, the results of the present research can be used to inform future decisions regarding language policy at the University of Fort Hare.
- Full Text:
- Date Issued: 2004
Becoming a journalist : a study into the professional socialisation and training of entry-level journalists at the Cape Argus newspaper
- Authors: Maughan, Karyn
- Date: 2004
- Subjects: Argus (Cape Town, South Africa) , Journalism -- Study and teaching -- South Africa , Journalists -- Training of -- South Africa , Journalists -- Selection and appointment -- South Africa , Journalists -- Education -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3507 , http://hdl.handle.net/10962/d1007553 , Argus (Cape Town, South Africa) , Journalism -- Study and teaching -- South Africa , Journalists -- Training of -- South Africa , Journalists -- Selection and appointment -- South Africa , Journalists -- Education -- South Africa
- Description: This thesis attempts to examine the construction of 'professionalism' within the newsroom of the Cape Argus, an English-medium newspaper in post-apartheid South Africa. It is a qualitative study which tries to evaluate how a particular mainstream media discourse of 'professionalism' is enacted and struggled over in the attitudes, behaviour and perceptions of entry-level journalists and news managers at the newspaper. It asks what the process of 'becoming a journalist' requires of entry-level journalists in terms of their previous education and personal qualities - and examines the newsroom strategies employed by news managers when entry-level journalists do not meet these particular requirements. This thesis looks at how the pressures of operating a daily English-language commercial newspaper may shape both the 'professional' expectations of news managers and their ability to positively contribute to entry-level journalists' 'newsroom training'. In attempting to examine the nature of journalistic 'professionalism', this study explores the ideology of knowledge construction within mainstream South African media. Operating from a 'radical democratic' perspective of journalism, which prioritises journalism as a vehicle for diverse social, cultural and political expression, this thesis suggests that South African media education needs to enable journalism students' understanding of the ideological construction of journalistic 'professionalism'.
- Full Text:
- Date Issued: 2004
- Authors: Maughan, Karyn
- Date: 2004
- Subjects: Argus (Cape Town, South Africa) , Journalism -- Study and teaching -- South Africa , Journalists -- Training of -- South Africa , Journalists -- Selection and appointment -- South Africa , Journalists -- Education -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3507 , http://hdl.handle.net/10962/d1007553 , Argus (Cape Town, South Africa) , Journalism -- Study and teaching -- South Africa , Journalists -- Training of -- South Africa , Journalists -- Selection and appointment -- South Africa , Journalists -- Education -- South Africa
- Description: This thesis attempts to examine the construction of 'professionalism' within the newsroom of the Cape Argus, an English-medium newspaper in post-apartheid South Africa. It is a qualitative study which tries to evaluate how a particular mainstream media discourse of 'professionalism' is enacted and struggled over in the attitudes, behaviour and perceptions of entry-level journalists and news managers at the newspaper. It asks what the process of 'becoming a journalist' requires of entry-level journalists in terms of their previous education and personal qualities - and examines the newsroom strategies employed by news managers when entry-level journalists do not meet these particular requirements. This thesis looks at how the pressures of operating a daily English-language commercial newspaper may shape both the 'professional' expectations of news managers and their ability to positively contribute to entry-level journalists' 'newsroom training'. In attempting to examine the nature of journalistic 'professionalism', this study explores the ideology of knowledge construction within mainstream South African media. Operating from a 'radical democratic' perspective of journalism, which prioritises journalism as a vehicle for diverse social, cultural and political expression, this thesis suggests that South African media education needs to enable journalism students' understanding of the ideological construction of journalistic 'professionalism'.
- Full Text:
- Date Issued: 2004
Biochemical characterization of plasmodium falciparum heat shock protein 70
- Matambo, Tonderayi Sylvester
- Authors: Matambo, Tonderayi Sylvester
- Date: 2004
- Subjects: Plasmodium falciparum , Malaria -- Prevention , Protein folding , Proteins -- Purification , Heat shock proteins
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4134 , http://hdl.handle.net/10962/d1015767
- Description: Plamodium falciparum heat shock protein (PfHsp70) is believed to be involved in the cytoprotection of the malaria parasite through its action as a molecular chaperone. Bioinformatic analysis reveal that PfHsp70 consists of the three canonical Hsp70 domains; an ATPase domain of 45 kDa, Substrate binding domain of 15 kDa and a C-terminal domain of 10 kDa. At the C-terminus there is a GGMP repeat motif that is commonly found in Hsp70s of parasitic origins. Plasmodium falciparum genome is 80% A-T rich, making it difficult to recombinantly express its proteins in Escherhia coli (E. coli) as a result of rare codon usage. In this study we carried out experiments to improve expression in E. coli by inserting the PfHsp70 coding region into the pQE30 expression vector. However multiple bands were detected by Western analysis, probably due to the presence of rare codons. The RIG plasmid, which encodes tRNAs for rare codons in particular Arg (AGA/AGG), Ile (AUA) and Gly (GGA) was engineered into the E. coli strain resulting in production of full length PfHsp70. Purification was achieved through Ni²⁺ Chelating sepharose under denaturing conditions. PfHsp70 was found to have a very low basal ATPase activity of 0.262 ± 0.05 nmoles/min/mg of protein. In the presence of reduced and carboxymethylated lactalbumin (RCMLA) a 11-fold increase in ATPase activity was noted whereas in the presence of both RCMLA and Trypanosoma cruzi DnaJ (Tcj2) a 16-fold was achieved. For ATP hydrolysis kcat value of 0.003 min⁻¹ was obtained whereas for ADP release a greater kcat value of 0.8 min⁻¹ was obtained. These results indicated that rate of ATP hydrolysis maybe the rate-determining step in the ATPase cycle of PfHsp70.
- Full Text:
- Date Issued: 2004
- Authors: Matambo, Tonderayi Sylvester
- Date: 2004
- Subjects: Plasmodium falciparum , Malaria -- Prevention , Protein folding , Proteins -- Purification , Heat shock proteins
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4134 , http://hdl.handle.net/10962/d1015767
- Description: Plamodium falciparum heat shock protein (PfHsp70) is believed to be involved in the cytoprotection of the malaria parasite through its action as a molecular chaperone. Bioinformatic analysis reveal that PfHsp70 consists of the three canonical Hsp70 domains; an ATPase domain of 45 kDa, Substrate binding domain of 15 kDa and a C-terminal domain of 10 kDa. At the C-terminus there is a GGMP repeat motif that is commonly found in Hsp70s of parasitic origins. Plasmodium falciparum genome is 80% A-T rich, making it difficult to recombinantly express its proteins in Escherhia coli (E. coli) as a result of rare codon usage. In this study we carried out experiments to improve expression in E. coli by inserting the PfHsp70 coding region into the pQE30 expression vector. However multiple bands were detected by Western analysis, probably due to the presence of rare codons. The RIG plasmid, which encodes tRNAs for rare codons in particular Arg (AGA/AGG), Ile (AUA) and Gly (GGA) was engineered into the E. coli strain resulting in production of full length PfHsp70. Purification was achieved through Ni²⁺ Chelating sepharose under denaturing conditions. PfHsp70 was found to have a very low basal ATPase activity of 0.262 ± 0.05 nmoles/min/mg of protein. In the presence of reduced and carboxymethylated lactalbumin (RCMLA) a 11-fold increase in ATPase activity was noted whereas in the presence of both RCMLA and Trypanosoma cruzi DnaJ (Tcj2) a 16-fold was achieved. For ATP hydrolysis kcat value of 0.003 min⁻¹ was obtained whereas for ADP release a greater kcat value of 0.8 min⁻¹ was obtained. These results indicated that rate of ATP hydrolysis maybe the rate-determining step in the ATPase cycle of PfHsp70.
- Full Text:
- Date Issued: 2004
Black Business Executive Circle Award
- Date: 2004 , 2022-10-03
- Subjects: Bam, Brigalia , Bam, Brigalia -- Awards
- Language: English
- Type: realia
- Identifier: http://hdl.handle.net/10948/56722 , vital:57144
- Description: Black Business Executive Circle Award in recognition of Dr. Brigalia Bam for her outstanding performance in managing the 2004 elections. , Donated/gifted to Nelson Mandela University Archives , Forms part of: Brigalia Bam collection
- Full Text: false
- Date Issued: 2004
- Date: 2004 , 2022-10-03
- Subjects: Bam, Brigalia , Bam, Brigalia -- Awards
- Language: English
- Type: realia
- Identifier: http://hdl.handle.net/10948/56722 , vital:57144
- Description: Black Business Executive Circle Award in recognition of Dr. Brigalia Bam for her outstanding performance in managing the 2004 elections. , Donated/gifted to Nelson Mandela University Archives , Forms part of: Brigalia Bam collection
- Full Text: false
- Date Issued: 2004
Burial practices, African women, and Islam in the Eastern Cape province, South Africa
- Authors: Sesanti, Simphiwe Olicius
- Date: 2004
- Subjects: Islamic funeral rites and ceremonies -- South Africa -- Eastern Cape , Muslim women -- South Africa -- Eastern Cape , Mourning custom -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:10998 , http://hdl.handle.net/10948/346 , Islamic funeral rites and ceremonies -- South Africa -- Eastern Cape , Muslim women -- South Africa -- Eastern Cape , Mourning custom -- South Africa -- Eastern Cape
- Description: Early in 2003, African Muslims in Uitenhage’s township, Kwa-Nobuhle, learnt that Muslim women, led by Sheikh Nceba Salamntu, in South Africa’s Port Elizabeth New Brighton township, were allowed, contrary to previous practice, to follow a funeral procession right up to the graveyard. The resultant discomfort on the one hand, and excitement on the other caused by this event among Muslims in the township, forms the basis of this research. It gives focus to Muslim women, the ones most affected by their customary restriction from the gravesites. The researchi exposes the basis for women’s exclusion from funeral processions in the Muslim community. It was established that many of these Muslim women who challenged the practice were converts from Christianity to Islam. One of the bases for their action was that they were passive recipients of Islam. Furthermore, it was found that the exclusion of women from the funeral procession has no basis in Islamic writings.
- Full Text:
- Date Issued: 2004
- Authors: Sesanti, Simphiwe Olicius
- Date: 2004
- Subjects: Islamic funeral rites and ceremonies -- South Africa -- Eastern Cape , Muslim women -- South Africa -- Eastern Cape , Mourning custom -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:10998 , http://hdl.handle.net/10948/346 , Islamic funeral rites and ceremonies -- South Africa -- Eastern Cape , Muslim women -- South Africa -- Eastern Cape , Mourning custom -- South Africa -- Eastern Cape
- Description: Early in 2003, African Muslims in Uitenhage’s township, Kwa-Nobuhle, learnt that Muslim women, led by Sheikh Nceba Salamntu, in South Africa’s Port Elizabeth New Brighton township, were allowed, contrary to previous practice, to follow a funeral procession right up to the graveyard. The resultant discomfort on the one hand, and excitement on the other caused by this event among Muslims in the township, forms the basis of this research. It gives focus to Muslim women, the ones most affected by their customary restriction from the gravesites. The researchi exposes the basis for women’s exclusion from funeral processions in the Muslim community. It was established that many of these Muslim women who challenged the practice were converts from Christianity to Islam. One of the bases for their action was that they were passive recipients of Islam. Furthermore, it was found that the exclusion of women from the funeral procession has no basis in Islamic writings.
- Full Text:
- Date Issued: 2004
Capsule immobilisation of sulphate-reducing bacteria and application in disarticulated systems
- Authors: Sanyahumbi, Douglas
- Date: 2004
- Subjects: Sulfur bacteria , Water -- Purification -- Biological treatment
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3935 , http://hdl.handle.net/10962/d1003994
- Description: Biotechnology of sulphate reducing bacteria has developed rapidly in recent years with the recognition of their extensive and diverse biocatalytic potential. However, their application in a number of areas has been constrained due to problems including poor cell retention within the continuous bioprocess reactor environment, and contamination of the treated stream with residual organic feed components and cell biomass. These problems have so far excluded the application of biological sulphate reduction in the treatment of ‘clean’ inorganic waste streams where components such as sulphate, acidity and heavy metal contamination require treatment. This study investigated the effective immobilisation of sulphate reducing bacterial cultures and proposed that the disarticulation of the electron donor and carbon source supply using such systems would create the basis for their application in the treatment of ‘clean’ inorganic waste streams. A functional and stable sulphate reducing culture was selected and following evaluation using a number of techniques, was immobilised by encapsulation within a calcium-alginate-xanthum gum membrane to give robust capsules with good sulphate reduction activity. The concept of disarticulation was investigated in a swing-back cycle where the carbon source was excluded and the electron donor supplied in the form of hydrogen gas in a continuous up-flow capsule-packed column reactor. Following a period of operation in this mode (4-12 days), the system was swung back to a carbon feed to supply requirements of cell maintenance (2-3 days). Three types of synthetic ‘clean’ inorganic waste stream treatments were investigated, including sulphate removal, neutralisation of acidity and heavy metal (copper and lead) removal. The results showed: • Sulphate removal at a rate of 50 mg SO₄²⁻L/day/g initial wet mass of capsules during three 4-day cycles of electron donor phase. This was comparable to the performance of free cell systems; • Neutralisation of acidity where influent pH values of 2.4 and 4.0 were elevated to above pH 7.5; • Copper removal of 99 and 85 % was achieved with initial copper concentrations of 2 and 60 mg/L respectively; • Percentage lead removal values of 49 and 78 % were achieved; This first report on the application of the concept of capsular immobilisation and disarticulation in the treatment of ‘clean’ inorganic waste streams will require future studies in order to extend the development of the full potential of the concept.
- Full Text:
- Date Issued: 2004
- Authors: Sanyahumbi, Douglas
- Date: 2004
- Subjects: Sulfur bacteria , Water -- Purification -- Biological treatment
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3935 , http://hdl.handle.net/10962/d1003994
- Description: Biotechnology of sulphate reducing bacteria has developed rapidly in recent years with the recognition of their extensive and diverse biocatalytic potential. However, their application in a number of areas has been constrained due to problems including poor cell retention within the continuous bioprocess reactor environment, and contamination of the treated stream with residual organic feed components and cell biomass. These problems have so far excluded the application of biological sulphate reduction in the treatment of ‘clean’ inorganic waste streams where components such as sulphate, acidity and heavy metal contamination require treatment. This study investigated the effective immobilisation of sulphate reducing bacterial cultures and proposed that the disarticulation of the electron donor and carbon source supply using such systems would create the basis for their application in the treatment of ‘clean’ inorganic waste streams. A functional and stable sulphate reducing culture was selected and following evaluation using a number of techniques, was immobilised by encapsulation within a calcium-alginate-xanthum gum membrane to give robust capsules with good sulphate reduction activity. The concept of disarticulation was investigated in a swing-back cycle where the carbon source was excluded and the electron donor supplied in the form of hydrogen gas in a continuous up-flow capsule-packed column reactor. Following a period of operation in this mode (4-12 days), the system was swung back to a carbon feed to supply requirements of cell maintenance (2-3 days). Three types of synthetic ‘clean’ inorganic waste stream treatments were investigated, including sulphate removal, neutralisation of acidity and heavy metal (copper and lead) removal. The results showed: • Sulphate removal at a rate of 50 mg SO₄²⁻L/day/g initial wet mass of capsules during three 4-day cycles of electron donor phase. This was comparable to the performance of free cell systems; • Neutralisation of acidity where influent pH values of 2.4 and 4.0 were elevated to above pH 7.5; • Copper removal of 99 and 85 % was achieved with initial copper concentrations of 2 and 60 mg/L respectively; • Percentage lead removal values of 49 and 78 % were achieved; This first report on the application of the concept of capsular immobilisation and disarticulation in the treatment of ‘clean’ inorganic waste streams will require future studies in order to extend the development of the full potential of the concept.
- Full Text:
- Date Issued: 2004
Catalytic activity of iron and cobalt phthalocyanine complexes towards the oxidation of cyclohexene using tert-butylhydroperoxide and chloroperoxybenzoic acid
- Sehlotho, Nthapo, Nyokong, Tebello
- Authors: Sehlotho, Nthapo , Nyokong, Tebello
- Date: 2004
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/290257 , vital:56729 , xlink:href="https://doi.org/10.1016/j.molcata.2003.08.014"
- Description: Cyclohexene oxidation using tert-butylhydroperoxide (TBHP) or chloroperoxybenzoic acid (CPBA) in the presence of iron(II) polychlorophthalocyanine (Cl16PcFe), iron(II) phthalocyanine (PcFe) and cobalt(II) phthalocyanine (PcCo), results in the formation of the following products: cyclohexene oxide, 2-cyclohexene-1-ol and 2-cyclohexene-1-one. Adipic acid was also formed after long reaction times. The selectivity for 2-cyclohexene-1-one is favoured when Cl16PcFe or PcCo catalysts are employed, while PcFe is selective towards the formation of 2-cyclohexene-1-ol. The Cl16PcFe catalyst is transformed into a μ-oxo dimer (Cl16PcFeIIIOIIIFePcCl16) during the oxidation process. The catalytic process using the unsubstituted PcCoII and PcFeII catalysts involved PcMIII species as an intermediate. The active form of the Cl16PcFe catalyst was stable to degradation in that it was still active even after 4 weeks of continued catalysis.
- Full Text:
- Date Issued: 2004
- Authors: Sehlotho, Nthapo , Nyokong, Tebello
- Date: 2004
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/290257 , vital:56729 , xlink:href="https://doi.org/10.1016/j.molcata.2003.08.014"
- Description: Cyclohexene oxidation using tert-butylhydroperoxide (TBHP) or chloroperoxybenzoic acid (CPBA) in the presence of iron(II) polychlorophthalocyanine (Cl16PcFe), iron(II) phthalocyanine (PcFe) and cobalt(II) phthalocyanine (PcCo), results in the formation of the following products: cyclohexene oxide, 2-cyclohexene-1-ol and 2-cyclohexene-1-one. Adipic acid was also formed after long reaction times. The selectivity for 2-cyclohexene-1-one is favoured when Cl16PcFe or PcCo catalysts are employed, while PcFe is selective towards the formation of 2-cyclohexene-1-ol. The Cl16PcFe catalyst is transformed into a μ-oxo dimer (Cl16PcFeIIIOIIIFePcCl16) during the oxidation process. The catalytic process using the unsubstituted PcCoII and PcFeII catalysts involved PcMIII species as an intermediate. The active form of the Cl16PcFe catalyst was stable to degradation in that it was still active even after 4 weeks of continued catalysis.
- Full Text:
- Date Issued: 2004
Changes in energy use patterns in the Bushbuckridge Lowveld of the Limpopo Province, South Africa: eleven years on
- Authors: Madubansi, Mainza
- Date: 2004
- Subjects: Energy consumption -- South Africa -- Limpopo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4756 , http://hdl.handle.net/10962/d1007139 , Energy consumption -- South Africa -- Limpopo
- Description: This research reports on the energy transition that has taken place in the Bushbuckridge district between 1991 and 2002. It is a follow up to a similar study that was done in 1991 investigating a number of aspects of energy use. It uses the 1991 study as a baseline and aims to explore how the passage of time, growth of the local population and changes in incomes and the availability of fuels have affected the patterns of fuel use in the region in the past eleven years. A structured interview approach was used for most aspects of the study. The interview schedule included sections on types, amounts and reasons for use and non-use of 13 different energy sources as well as data on income levels, household size and other economic parameters. Analysis of the consumption patterns of the different fuel types revealed that between the two survey periods, households in the sample settlements went through some pronounced changes in patterns of fuel use, particularly those that had acquired electricity. The introduction of electricity in the region had certainly played a major role in spurring the energy transition. Fuels that were previously used for lighting, powering entertainment appliances and refrigeration had been displaced by electricity. In terms of cooking and other thermal application, however, the vast majority of households in all the sample settlements continued using fuelwood and complemented it to a lesser extent with paraffin and electricity. In both surveys, the use or non-use of available fuels in the region was influenced by several factors. Common reasons for non-use of certain fuel types included expense, lack of appliances, the risk involved in using such fuels and the preference for other fuels. Reasons for use were mainly related to the low cost of the fuel and the fuel’s ability to meet particular end uses. Although incomes in the region had increased between 1991 and 2002, they were still below the poverty line. Activities from which households obtained their income remained the same. Old age pension, migrant wages and the informal sector remained the largest contributors of income to most households. Other indicators of relative wealth and poverty, like ownership of vehicles, bicycles and beds remained the same.
- Full Text:
- Date Issued: 2004
- Authors: Madubansi, Mainza
- Date: 2004
- Subjects: Energy consumption -- South Africa -- Limpopo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4756 , http://hdl.handle.net/10962/d1007139 , Energy consumption -- South Africa -- Limpopo
- Description: This research reports on the energy transition that has taken place in the Bushbuckridge district between 1991 and 2002. It is a follow up to a similar study that was done in 1991 investigating a number of aspects of energy use. It uses the 1991 study as a baseline and aims to explore how the passage of time, growth of the local population and changes in incomes and the availability of fuels have affected the patterns of fuel use in the region in the past eleven years. A structured interview approach was used for most aspects of the study. The interview schedule included sections on types, amounts and reasons for use and non-use of 13 different energy sources as well as data on income levels, household size and other economic parameters. Analysis of the consumption patterns of the different fuel types revealed that between the two survey periods, households in the sample settlements went through some pronounced changes in patterns of fuel use, particularly those that had acquired electricity. The introduction of electricity in the region had certainly played a major role in spurring the energy transition. Fuels that were previously used for lighting, powering entertainment appliances and refrigeration had been displaced by electricity. In terms of cooking and other thermal application, however, the vast majority of households in all the sample settlements continued using fuelwood and complemented it to a lesser extent with paraffin and electricity. In both surveys, the use or non-use of available fuels in the region was influenced by several factors. Common reasons for non-use of certain fuel types included expense, lack of appliances, the risk involved in using such fuels and the preference for other fuels. Reasons for use were mainly related to the low cost of the fuel and the fuel’s ability to meet particular end uses. Although incomes in the region had increased between 1991 and 2002, they were still below the poverty line. Activities from which households obtained their income remained the same. Old age pension, migrant wages and the informal sector remained the largest contributors of income to most households. Other indicators of relative wealth and poverty, like ownership of vehicles, bicycles and beds remained the same.
- Full Text:
- Date Issued: 2004
Characterisation of the J domain aminoacid residues important for the interaction of DNAJ-like proteins with HSP70 chaperones
- Authors: Hennessy, Fritha
- Date: 2004
- Subjects: Heat shock proteins Protein folding Proteins -- Analysis Proteins -- Structure Molecular chaperones
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3937 , http://hdl.handle.net/10962/d1003996
- Description: The 70 kDa heat shock proteins (Hsp70s) are vital for normal protein folding, as they stabilise the unfolded state of nascent polypeptides, allowing these sufficient time to attain a correct tertiary structure. Hsp70s are aided by the DnaJ-like family of proteins, which interact with Hsp70s in order to enhance the chaperone activity of these proteins. DnaJ-like proteins contain a J domain, a seventy amino acid domain consisting of four α-helices, which is defined by the presence of an invariant tripeptide of histidine, proline and aspartic acid (HPD motif). This motif is key to the interaction between DnaJ-like proteins and Hsp70s. This thesis has focused on determining the presence of other conserved residues in the J domain and their role in mediating the interaction of DnaJ-like proteins with partner Hsp70s. DnaJ-like proteins from Agrobacterium tumefaciens RUOR were isolated and used as a model system. A. tumefaciens DnaJ (Agt DnaJ) was able to replace the lack of E. coli DnaJ in an E. coli null mutant strain, however, additional A. tumefaciens DnaJ-like proteins Agt DjC1/DjlA, Agt DjC2 and Agt DjC5 were unable to complement for the lack of E. coli DnaJ. Replacement of the Agt DnaJ J domain with J domains from these proteins resulted in non-functional chimeric proteins, despite some sequence conservation. The kinetics of the basal specific ATPase activity of Agt DnaK, and its ability to have this activity stimulated by Agt DnaJ and Agt DnaJ-H33Q were also investigated. Stimulation of the ATPase activity by Agt DnaJ ranged between 1.5 to 2 fold, but Agt DnaJ-H33Q was unable to stimulate the basal ATPase activity. Conserved amino acids in the J domain were identified in silico, and these residues were substituted in the J domain of Agt DnaJ. The ability of these derivative proteins to replace E. coli DnaJ was investigated. Alterations in the HPD motif gave rise to proteins unable to complement for lack of E. coli DnaJ, consistent with literature. Agt DnaJ-R26A was unable to replace E. coli DnaJ suggesting that Arg26 could be key to the interaction with partner Hsp70s. Agt DnaJ-D59A was unable to replace E. coli DnaJ; substitutions in Asp59 have not previously been shown to impact on the function of DnaJ. Substituting Arg63 in Agt DnaJ abrogated the levels of complementation. Substitution of several structural residues was also found to disrupt the in vivo function of Agt DnaJ suggesting that the maintenance of the structural integrity of the J domain was important for function. This study has identified a number of residues critical to the structure and function of the J domain of Agt DnaJ, and potentially of general importance as molecular determinants for DnaJ-Hsp70 interaction.
- Full Text:
- Date Issued: 2004
- Authors: Hennessy, Fritha
- Date: 2004
- Subjects: Heat shock proteins Protein folding Proteins -- Analysis Proteins -- Structure Molecular chaperones
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3937 , http://hdl.handle.net/10962/d1003996
- Description: The 70 kDa heat shock proteins (Hsp70s) are vital for normal protein folding, as they stabilise the unfolded state of nascent polypeptides, allowing these sufficient time to attain a correct tertiary structure. Hsp70s are aided by the DnaJ-like family of proteins, which interact with Hsp70s in order to enhance the chaperone activity of these proteins. DnaJ-like proteins contain a J domain, a seventy amino acid domain consisting of four α-helices, which is defined by the presence of an invariant tripeptide of histidine, proline and aspartic acid (HPD motif). This motif is key to the interaction between DnaJ-like proteins and Hsp70s. This thesis has focused on determining the presence of other conserved residues in the J domain and their role in mediating the interaction of DnaJ-like proteins with partner Hsp70s. DnaJ-like proteins from Agrobacterium tumefaciens RUOR were isolated and used as a model system. A. tumefaciens DnaJ (Agt DnaJ) was able to replace the lack of E. coli DnaJ in an E. coli null mutant strain, however, additional A. tumefaciens DnaJ-like proteins Agt DjC1/DjlA, Agt DjC2 and Agt DjC5 were unable to complement for the lack of E. coli DnaJ. Replacement of the Agt DnaJ J domain with J domains from these proteins resulted in non-functional chimeric proteins, despite some sequence conservation. The kinetics of the basal specific ATPase activity of Agt DnaK, and its ability to have this activity stimulated by Agt DnaJ and Agt DnaJ-H33Q were also investigated. Stimulation of the ATPase activity by Agt DnaJ ranged between 1.5 to 2 fold, but Agt DnaJ-H33Q was unable to stimulate the basal ATPase activity. Conserved amino acids in the J domain were identified in silico, and these residues were substituted in the J domain of Agt DnaJ. The ability of these derivative proteins to replace E. coli DnaJ was investigated. Alterations in the HPD motif gave rise to proteins unable to complement for lack of E. coli DnaJ, consistent with literature. Agt DnaJ-R26A was unable to replace E. coli DnaJ suggesting that Arg26 could be key to the interaction with partner Hsp70s. Agt DnaJ-D59A was unable to replace E. coli DnaJ; substitutions in Asp59 have not previously been shown to impact on the function of DnaJ. Substituting Arg63 in Agt DnaJ abrogated the levels of complementation. Substitution of several structural residues was also found to disrupt the in vivo function of Agt DnaJ suggesting that the maintenance of the structural integrity of the J domain was important for function. This study has identified a number of residues critical to the structure and function of the J domain of Agt DnaJ, and potentially of general importance as molecular determinants for DnaJ-Hsp70 interaction.
- Full Text:
- Date Issued: 2004
Cleaning of fouled membranes using enzymes from a sulphidogenic bioreactor
- Authors: Melamane, Xolisa
- Date: 2004
- Subjects: Membrane filters , Membrane filters -- Fouling , Enzymes -- Biotechnology , Enzymes -- Purification , Water -- Purification -- Membrane filtration , Ultrafiltration
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4133 , http://hdl.handle.net/10962/d1015764
- Description: Maintenance of membrane performance requires inevitable cleaning or defouling of fouled membranes. Membrane cleaning using enzymes such as proteases, lipases, α-glucosidases from a sulphidogenic bioreactor was investigated. At first, dilute and concentrated enzyme extract were prepared form the sulphidogenic pellet. Enzyme assays on 0.5 % azocaisen, 1 % triacetin and 1 mg/ml ρ-nitrophenyl-α-D-glucopyranoside were performed using the concentrated enzyme extract (0 – 200 mg/ml). For membrane fouling, an abattoir effluent was obtained from Ostritech Pty (Ltd), Grahamstown, South Africa. The effluent was characterised for presence of potential foulants such as lipids, proteins, amino acids and carbohydrates. Static fouling of polysulphone membranes (0.22 μm, 47 mm) was then performed using the abattoir effluent. Cleaning of the fouled membranes was also performed using at first the dilute and then the concentrated form (200 mg/ml) of enzyme extracts. Qualitative and quantitative biochemical analysis for proteins, lipids and carbohydrates was performed to ascertain the presence of foulants on polysulphone membranes and their removal by dilute or concentrated enzyme extracts. The ability of dilute enzyme extracts to remove proteins lipids, and carbohydrates fouling capillary UF membrane module; their ability to restore permeate fluxes and transmembrane pressure after cleaning/defouling was also investigated. Permeate volumes from this UF membrane module were analysed for protein, amino acids, lipids, and carbohydrates concentrations after fouling and defouling. Fouling was further characterized by standard blocking, cake filtration and pore blocking models using stirred UF cell and polyethersulphone membranes with MWCO of 30 000, 100 000 and 300 000. After characterization of fouling, polyethersulphone membranes with MWCO of 30 000 and 300 000 were defouled using the concentrated enzyme extract (100 mg ml). Enzyme activities at 200 mg/ml of enzyme concentration were 8.071 IU, 86.71 IU and 789.02 IU for proteases, lipases and α-glucosidases. The abattoir effluent contained 553 μg/ml of lipid, 301 μg/ml of protein, 141 μg/ml of total carbohydrate, and 0.63 μg/ml of total reducing sugars. Proteins, lipids and carbohydrates fouling polysulphone membranes after a day were removed by 23.4 %, when a dilute enzyme was used. A concentrated enzyme extract of 200 mg/ml was able to remove proteins, lipids and carbohydrates up to 5 days of fouling by 100 %, 82 %, 71 %, 68 % and 76 % respectively. Defouling of dynamically fouled capillary ultrafiltration membranes using sulphidogenic proteases was successful at pH 10, 37°C, within 1 hour. Sulphidogenic proteases activity was 2.1 U/ml and flux Recovery (FR %) was 64. Characterization of fouling revealed that proteins and lipids were major foulants while low concentration of carbohydrates fouled polyethersulphone membranes. Fouling followed standard blocking for 10 minutes in all the membranes; afterwards fouling adopted cake filtration model for membranes with 30 000 MWCO and pore blocking model for membranes with 300 000 MWCO. A concentration of 100 mg/ml of enzyme extract was able to remove fouling from membranes with MWCO of 30 000. Defouling membranes that followed pore blocking model i.e. 300 000 MWCO was not successful due to a mass transfer problem. From the results of defouling of 30 000 and 300 000 MWCO it was concluded that defouling of cake layer fouling (30 000 MWCO) was successful while defouling of pore blocking fouling was unsuccessful due to a mass transfer problem. The ratio of enzymes present in the enzyme extract when calculated based on enzymatic activity for proteases, lipases and α-glucosidases was 1.1 %, 11 % and 87.9 %. It was hypothesized that apart from proteases, lipases, α and β-glucosidases; phosphatases, sulphatases, amonipeptidases etc. from a sulphidogenic bioreactor clean or defoul cake layer fouling by organic foulants and pore blocking fouling provided the mass transfer problem is solved. However, concentration of enzymes from a sulphidogenic bioreactor has not been optimized yet. Other methods of concentrating the enzyme extract can be investigated for example use of organic solvents.
- Full Text:
- Date Issued: 2004
- Authors: Melamane, Xolisa
- Date: 2004
- Subjects: Membrane filters , Membrane filters -- Fouling , Enzymes -- Biotechnology , Enzymes -- Purification , Water -- Purification -- Membrane filtration , Ultrafiltration
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4133 , http://hdl.handle.net/10962/d1015764
- Description: Maintenance of membrane performance requires inevitable cleaning or defouling of fouled membranes. Membrane cleaning using enzymes such as proteases, lipases, α-glucosidases from a sulphidogenic bioreactor was investigated. At first, dilute and concentrated enzyme extract were prepared form the sulphidogenic pellet. Enzyme assays on 0.5 % azocaisen, 1 % triacetin and 1 mg/ml ρ-nitrophenyl-α-D-glucopyranoside were performed using the concentrated enzyme extract (0 – 200 mg/ml). For membrane fouling, an abattoir effluent was obtained from Ostritech Pty (Ltd), Grahamstown, South Africa. The effluent was characterised for presence of potential foulants such as lipids, proteins, amino acids and carbohydrates. Static fouling of polysulphone membranes (0.22 μm, 47 mm) was then performed using the abattoir effluent. Cleaning of the fouled membranes was also performed using at first the dilute and then the concentrated form (200 mg/ml) of enzyme extracts. Qualitative and quantitative biochemical analysis for proteins, lipids and carbohydrates was performed to ascertain the presence of foulants on polysulphone membranes and their removal by dilute or concentrated enzyme extracts. The ability of dilute enzyme extracts to remove proteins lipids, and carbohydrates fouling capillary UF membrane module; their ability to restore permeate fluxes and transmembrane pressure after cleaning/defouling was also investigated. Permeate volumes from this UF membrane module were analysed for protein, amino acids, lipids, and carbohydrates concentrations after fouling and defouling. Fouling was further characterized by standard blocking, cake filtration and pore blocking models using stirred UF cell and polyethersulphone membranes with MWCO of 30 000, 100 000 and 300 000. After characterization of fouling, polyethersulphone membranes with MWCO of 30 000 and 300 000 were defouled using the concentrated enzyme extract (100 mg ml). Enzyme activities at 200 mg/ml of enzyme concentration were 8.071 IU, 86.71 IU and 789.02 IU for proteases, lipases and α-glucosidases. The abattoir effluent contained 553 μg/ml of lipid, 301 μg/ml of protein, 141 μg/ml of total carbohydrate, and 0.63 μg/ml of total reducing sugars. Proteins, lipids and carbohydrates fouling polysulphone membranes after a day were removed by 23.4 %, when a dilute enzyme was used. A concentrated enzyme extract of 200 mg/ml was able to remove proteins, lipids and carbohydrates up to 5 days of fouling by 100 %, 82 %, 71 %, 68 % and 76 % respectively. Defouling of dynamically fouled capillary ultrafiltration membranes using sulphidogenic proteases was successful at pH 10, 37°C, within 1 hour. Sulphidogenic proteases activity was 2.1 U/ml and flux Recovery (FR %) was 64. Characterization of fouling revealed that proteins and lipids were major foulants while low concentration of carbohydrates fouled polyethersulphone membranes. Fouling followed standard blocking for 10 minutes in all the membranes; afterwards fouling adopted cake filtration model for membranes with 30 000 MWCO and pore blocking model for membranes with 300 000 MWCO. A concentration of 100 mg/ml of enzyme extract was able to remove fouling from membranes with MWCO of 30 000. Defouling membranes that followed pore blocking model i.e. 300 000 MWCO was not successful due to a mass transfer problem. From the results of defouling of 30 000 and 300 000 MWCO it was concluded that defouling of cake layer fouling (30 000 MWCO) was successful while defouling of pore blocking fouling was unsuccessful due to a mass transfer problem. The ratio of enzymes present in the enzyme extract when calculated based on enzymatic activity for proteases, lipases and α-glucosidases was 1.1 %, 11 % and 87.9 %. It was hypothesized that apart from proteases, lipases, α and β-glucosidases; phosphatases, sulphatases, amonipeptidases etc. from a sulphidogenic bioreactor clean or defoul cake layer fouling by organic foulants and pore blocking fouling provided the mass transfer problem is solved. However, concentration of enzymes from a sulphidogenic bioreactor has not been optimized yet. Other methods of concentrating the enzyme extract can be investigated for example use of organic solvents.
- Full Text:
- Date Issued: 2004
Comparison of two granulation processes with the view to reduce manufacturing cost
- Authors: Maclean, Aldritt Allister
- Date: 2004
- Subjects: Drugs -- Granulation , Tablets (Medicine) , Pharmaceutical industry
- Language: English
- Type: Thesis , Masters , MTech (Chemistry)
- Identifier: vital:10980 , http://hdl.handle.net/10948/210 , Drugs -- Granulation , Tablets (Medicine) , Pharmaceutical industry
- Description: Aspen Pharmacare, one of the leading pharmaceutical manufacturers in South Africa has embarked on a programme of improving the production processes currently employed at their Port Elizabeth site. With the introduction of new technology at the site and the move towards globalization, it became imperative that Aspen remain competitive in the market. The product of interest in this research, Degoran Plus tablets, is one of the company’s leading brand sellers. Upon investigation, it became apparent that this product created opportunity for process improvement using the new technology. The manufacture of Degoran Plus entails granulation, compression and coating of the product. Most opportunity for improvement was possible in the granulation stage because of the laborious nature of the present process. Degoran Plus tablets had a history of analytical failures, especially with regard to the dissolution rate of the final product, as well as other quality related issues. The product was not considered to be a “through-runner”, which resulted in bad production output, due to continual repeats of not only analysis but also reworks in production. A strategic decision was taken to manufacture Degoran Plus using the Collette Gral granulator as the equipment offered superior mixing capability when compared to the Bear planetary granulator. It was assumed that the granulation process would result in more uniform distribution of the actives. Upon producing a better granule, a final product of superior quality would be attained. The validation protocol stipulates that three samples be taken and tested from the powder mix. Nine samples taken from granulated bulk are treated in the same manner. The validation protocol further stipulates that the first three batches manufactured utilise the new process, and tested according to the protocol. The results obtained from the analysis are evaluated statistically and a conclusion and recommendation were derived based on the evaluation.
- Full Text:
- Date Issued: 2004
- Authors: Maclean, Aldritt Allister
- Date: 2004
- Subjects: Drugs -- Granulation , Tablets (Medicine) , Pharmaceutical industry
- Language: English
- Type: Thesis , Masters , MTech (Chemistry)
- Identifier: vital:10980 , http://hdl.handle.net/10948/210 , Drugs -- Granulation , Tablets (Medicine) , Pharmaceutical industry
- Description: Aspen Pharmacare, one of the leading pharmaceutical manufacturers in South Africa has embarked on a programme of improving the production processes currently employed at their Port Elizabeth site. With the introduction of new technology at the site and the move towards globalization, it became imperative that Aspen remain competitive in the market. The product of interest in this research, Degoran Plus tablets, is one of the company’s leading brand sellers. Upon investigation, it became apparent that this product created opportunity for process improvement using the new technology. The manufacture of Degoran Plus entails granulation, compression and coating of the product. Most opportunity for improvement was possible in the granulation stage because of the laborious nature of the present process. Degoran Plus tablets had a history of analytical failures, especially with regard to the dissolution rate of the final product, as well as other quality related issues. The product was not considered to be a “through-runner”, which resulted in bad production output, due to continual repeats of not only analysis but also reworks in production. A strategic decision was taken to manufacture Degoran Plus using the Collette Gral granulator as the equipment offered superior mixing capability when compared to the Bear planetary granulator. It was assumed that the granulation process would result in more uniform distribution of the actives. Upon producing a better granule, a final product of superior quality would be attained. The validation protocol stipulates that three samples be taken and tested from the powder mix. Nine samples taken from granulated bulk are treated in the same manner. The validation protocol further stipulates that the first three batches manufactured utilise the new process, and tested according to the protocol. The results obtained from the analysis are evaluated statistically and a conclusion and recommendation were derived based on the evaluation.
- Full Text:
- Date Issued: 2004
Competition for attachment of aquaculture candidate probiotic and pathogenic bacteria on fish intestinal mucus:
- Vine, Niall G, Leukes, Winston D, Kaiser, Horst, Daya, Santylal, Baxter, Jeremy, Hecht, Thomas
- Authors: Vine, Niall G , Leukes, Winston D , Kaiser, Horst , Daya, Santylal , Baxter, Jeremy , Hecht, Thomas
- Date: 2004
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142819 , vital:38120 , DOI: 10.1111/j.1365-2761.2004.00542.x
- Description: Probiotics for aquaculture are generally only selected by their ability to produce antimicrobial metabolites; however, attachment to intestinal mucus is important in order to remain within the gut of its host. Five candidate probiotics (AP1–AP5), isolated from the clownfish, Amphiprion percula (Lacepéde), were examined for their ability to attach to fish intestinal mucus and compete with two pathogens, Aeromonas hydrophila and Vibrio alginolyticus. Two different radioactive isotopes were used to quantify competition between pathogens and probionts. Attachment of the pathogens was enhanced by the presence of the candidate probiotics. However, the addition of the candidate probiotics after the pathogens resulted in reduced pathogen attachment.
- Full Text:
- Date Issued: 2004
- Authors: Vine, Niall G , Leukes, Winston D , Kaiser, Horst , Daya, Santylal , Baxter, Jeremy , Hecht, Thomas
- Date: 2004
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142819 , vital:38120 , DOI: 10.1111/j.1365-2761.2004.00542.x
- Description: Probiotics for aquaculture are generally only selected by their ability to produce antimicrobial metabolites; however, attachment to intestinal mucus is important in order to remain within the gut of its host. Five candidate probiotics (AP1–AP5), isolated from the clownfish, Amphiprion percula (Lacepéde), were examined for their ability to attach to fish intestinal mucus and compete with two pathogens, Aeromonas hydrophila and Vibrio alginolyticus. Two different radioactive isotopes were used to quantify competition between pathogens and probionts. Attachment of the pathogens was enhanced by the presence of the candidate probiotics. However, the addition of the candidate probiotics after the pathogens resulted in reduced pathogen attachment.
- Full Text:
- Date Issued: 2004