Entomopathogenic fungi as control agents of Thaumatotibia leucotreta in citrus orchards: field efficacy and persistence
- Coombes, Candice A, Hill, Martin P, Moore, Sean D, Dames, Joanna F
- Authors: Coombes, Candice A , Hill, Martin P , Moore, Sean D , Dames, Joanna F
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/417956 , vital:71497 , xlink:href="https://doi.org/10.1007/s10526-016-9756-x"
- Description: Entomopathogenic fungal isolates Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae) strain G Ar 17 B3 and Metarhizium anisopliae (Metchnikoff) Sorokin (Hypocreales: Clavicipitaceae) strain FCM Ar 23 B3 have been identified as effective control agents of the important citrus pest Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae) wandering fifth instars under laboratory conditions. This study is the first report on the ability of these isolates to persist and reduce T. leucotreta infestation in commercial citrus orchards in South Africa. A reduction in pest infestation of between 28 and 82 % upon trial completion was reported. Both isolates were recovered from soil samples collected five months post-application with high host density and moderate to high soil moisture favouring recovery. Low soil moisture negatively influenced the persistence of both isolates and the control efficiency of B. bassiana. These results provide evidence and support for the future use of these isolates against T. leucotreta thus warranting further investigation.
- Full Text:
- Date Issued: 2016
- Authors: Coombes, Candice A , Hill, Martin P , Moore, Sean D , Dames, Joanna F
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/417956 , vital:71497 , xlink:href="https://doi.org/10.1007/s10526-016-9756-x"
- Description: Entomopathogenic fungal isolates Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae) strain G Ar 17 B3 and Metarhizium anisopliae (Metchnikoff) Sorokin (Hypocreales: Clavicipitaceae) strain FCM Ar 23 B3 have been identified as effective control agents of the important citrus pest Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae) wandering fifth instars under laboratory conditions. This study is the first report on the ability of these isolates to persist and reduce T. leucotreta infestation in commercial citrus orchards in South Africa. A reduction in pest infestation of between 28 and 82 % upon trial completion was reported. Both isolates were recovered from soil samples collected five months post-application with high host density and moderate to high soil moisture favouring recovery. Low soil moisture negatively influenced the persistence of both isolates and the control efficiency of B. bassiana. These results provide evidence and support for the future use of these isolates against T. leucotreta thus warranting further investigation.
- Full Text:
- Date Issued: 2016
Screening of entomopathogenic fungi against citrus mealybug, Plannococcus citri (Hemiptera: Pseudococcidae)
- Chartier FitzGerald, Veronique C, Hill, Martin P, Moore, Sean D, Dames, Joanna F
- Authors: Chartier FitzGerald, Veronique C , Hill, Martin P , Moore, Sean D , Dames, Joanna F
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/407059 , vital:70333 , xlink:href="https://hdl.handle.net/10520/EJC195093"
- Description: Planococcus citri (citrus mealybug) is a common and damaging citrus crop pest which has proven difficult to control using conventional methods, such as chemical pesticides and insect growth regulators, particularly late in the citrus growing season. The virulence of two entomopathogenic fungal species was studied in laboratory bioassays against the crawlers and adults of P. citri. Isolates of Metarhizium anisopliae and Beauveria bassiana, collected from citrus orchards in the Eastern Cape Province in South Africa, were verified using and molecular techniques. Mealybug bioassays were performed in 24-well plates. Beauveria bassiana (GAR 17 B3) and M. anisopliae (FCM AR 23 B3) isolates both resulted in 67.5 % mortality of mealybug crawlers and B. bassiana (GB AR 23 13 3) resulted in 64 % crawler mortality with concentrations of 1 x 107 conidia/ml. These three isolates were further tested in multipledose bioassays to determine the median lethal concentration (LC50), which were 5.29 x 105conidia/ml for the M. anisopliae isolate (FCM AR 23 B3), 4.25 x 106 conidia/ml for B. bassiana (GAR 17 B3), and 6.65 x 107 conidia/ml B. bassiana (GB AR 23 13 3) for crawlers, respectively. The results of this study suggested that two isolates (M. anisopliae FCM AR 23 B3 and B. bassiana GAR 17 B3) showed potential for further development as biological control agents against citrus mealybug. Further research would be required to determine their ability to perform under field conditions.
- Full Text:
- Date Issued: 2016
- Authors: Chartier FitzGerald, Veronique C , Hill, Martin P , Moore, Sean D , Dames, Joanna F
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/407059 , vital:70333 , xlink:href="https://hdl.handle.net/10520/EJC195093"
- Description: Planococcus citri (citrus mealybug) is a common and damaging citrus crop pest which has proven difficult to control using conventional methods, such as chemical pesticides and insect growth regulators, particularly late in the citrus growing season. The virulence of two entomopathogenic fungal species was studied in laboratory bioassays against the crawlers and adults of P. citri. Isolates of Metarhizium anisopliae and Beauveria bassiana, collected from citrus orchards in the Eastern Cape Province in South Africa, were verified using and molecular techniques. Mealybug bioassays were performed in 24-well plates. Beauveria bassiana (GAR 17 B3) and M. anisopliae (FCM AR 23 B3) isolates both resulted in 67.5 % mortality of mealybug crawlers and B. bassiana (GB AR 23 13 3) resulted in 64 % crawler mortality with concentrations of 1 x 107 conidia/ml. These three isolates were further tested in multipledose bioassays to determine the median lethal concentration (LC50), which were 5.29 x 105conidia/ml for the M. anisopliae isolate (FCM AR 23 B3), 4.25 x 106 conidia/ml for B. bassiana (GAR 17 B3), and 6.65 x 107 conidia/ml B. bassiana (GB AR 23 13 3) for crawlers, respectively. The results of this study suggested that two isolates (M. anisopliae FCM AR 23 B3 and B. bassiana GAR 17 B3) showed potential for further development as biological control agents against citrus mealybug. Further research would be required to determine their ability to perform under field conditions.
- Full Text:
- Date Issued: 2016
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