Exploration for sediment-hosted copper mineralization in Kaponda Prospect, Central African Copperbelt, Democratic Republic of Congo
- Authors: Kabunda, Ghislain Mwape
- Date: 2014
- Subjects: Copper mines and mining -- Central African Copperbelt (Congo and Zambia) , Sedimentation -- Central African Copperbelt (Congo and Zambia) , Prospecting -- Central African Copperbelt (Congo and Zambia) , Geological mapping -- Central African Copperbelt (Congo and Zambia) , Geochemistry -- Central African Copperbelt (Congo and Zambia) , Induced polarization
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5061 , http://hdl.handle.net/10962/d1013129
- Description: The Kaponda Prospect represents a surface of 915.8 km² located at about 10 km south of the town of Lubumbashi and 33km NW of Kasumbalesa in the Democratic Republic of Congo (DRC). It lies within Neoproterozoic sedimentary rocks of the Katangan Supergroup in the Central African Copperbelt (CACB). In this province, copper mineralization occurs at different stratigraphic level with different associated alteration. Mineralization is of multistage origin from synsedimentary, diagenetic to post orogenic. Since the discovery of the CACB in the early 20th century, several exploration techniques have been used to delineate Cu deposits. A review and application of these methods including remote sensing, geological mapping, geochemical and geophysical surveys, and drilling, gives an insight of their effectiveness and limitation before analyzing their results from the Kaponda Prospect. The geology and structure of the Prospect is represented by a series of two NW trending disharmonic tight anticlines, locally domal, with cores occupied by either Roan Group or “Grand Conglomerat” Formation. These anticlines are separated by an open syncline made of Kundelungu rocks. Two mains direction of faults are recognized, the NW and NE trending structures. The latter direction are normal transfer faults which can serve as conduit for mineralization. They are related to the late orogenic extension of the Lufilian belt. However NE trending faults are believed to be associated to the climax of Lufilian folding or represents synsedimentary intergrowth faults. Exploration approach for sediment-hosted Cu within Kaponda Prospect, take into account the integration of all information derived from different techniques. Remote sensing is used as aid to geology. Landsat and Google earth images show lineaments that corresponds to lithostratigraphy boundary and domal anticline. Geological mapping identified reduced horizons which can potentially host mineralization, whereas analysis of structure measurements reveals the geometry of fold and direction of its axial plane and hinge. Statistical methods such as the main + 2 standard deviation, the frequency histogram and probability plot, together with experiential method are used to constrain and define Cu and Co thresholds values in soil samples. It appears that in this region, log-probability plot and histogram methods combined with spatial representation and the experience of the region, are the best practice to constrain and separate geochemical background from anomaly data. Ground and airborne magnetic, and radiometric images show specific signatures which map alteration and particularly lithostratigraphy such as “Roan” Group, “Grand Conglomerat” unit, “Nguba” cap carbonates and “Kundelungu” siliciclastic units. Analysis of faults interpreted from geophysical maps identified three major directions: E-W, NE-SW and NW-SE. The E-W faults are also interpreted as normal transfer faults such NE-SW structures, consistent with regional geological map. Although pole-dipole array of induced polarization (IP) survey was directly targeting disseminated Cu sulphide, its results suffer in responding to graphitic rocks and barren pyrite. Only relative small chargeable bodies need to be tested in drilling follow-up. A total of 15 targets have been generated through re-interpretation and integration of both geological mapping and remote sensing, geochemical and geophysical data, as well as existing drilling. Specific recommendations of follow-up works are advised for each type of target.
- Full Text:
- Authors: Kabunda, Ghislain Mwape
- Date: 2014
- Subjects: Copper mines and mining -- Central African Copperbelt (Congo and Zambia) , Sedimentation -- Central African Copperbelt (Congo and Zambia) , Prospecting -- Central African Copperbelt (Congo and Zambia) , Geological mapping -- Central African Copperbelt (Congo and Zambia) , Geochemistry -- Central African Copperbelt (Congo and Zambia) , Induced polarization
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5061 , http://hdl.handle.net/10962/d1013129
- Description: The Kaponda Prospect represents a surface of 915.8 km² located at about 10 km south of the town of Lubumbashi and 33km NW of Kasumbalesa in the Democratic Republic of Congo (DRC). It lies within Neoproterozoic sedimentary rocks of the Katangan Supergroup in the Central African Copperbelt (CACB). In this province, copper mineralization occurs at different stratigraphic level with different associated alteration. Mineralization is of multistage origin from synsedimentary, diagenetic to post orogenic. Since the discovery of the CACB in the early 20th century, several exploration techniques have been used to delineate Cu deposits. A review and application of these methods including remote sensing, geological mapping, geochemical and geophysical surveys, and drilling, gives an insight of their effectiveness and limitation before analyzing their results from the Kaponda Prospect. The geology and structure of the Prospect is represented by a series of two NW trending disharmonic tight anticlines, locally domal, with cores occupied by either Roan Group or “Grand Conglomerat” Formation. These anticlines are separated by an open syncline made of Kundelungu rocks. Two mains direction of faults are recognized, the NW and NE trending structures. The latter direction are normal transfer faults which can serve as conduit for mineralization. They are related to the late orogenic extension of the Lufilian belt. However NE trending faults are believed to be associated to the climax of Lufilian folding or represents synsedimentary intergrowth faults. Exploration approach for sediment-hosted Cu within Kaponda Prospect, take into account the integration of all information derived from different techniques. Remote sensing is used as aid to geology. Landsat and Google earth images show lineaments that corresponds to lithostratigraphy boundary and domal anticline. Geological mapping identified reduced horizons which can potentially host mineralization, whereas analysis of structure measurements reveals the geometry of fold and direction of its axial plane and hinge. Statistical methods such as the main + 2 standard deviation, the frequency histogram and probability plot, together with experiential method are used to constrain and define Cu and Co thresholds values in soil samples. It appears that in this region, log-probability plot and histogram methods combined with spatial representation and the experience of the region, are the best practice to constrain and separate geochemical background from anomaly data. Ground and airborne magnetic, and radiometric images show specific signatures which map alteration and particularly lithostratigraphy such as “Roan” Group, “Grand Conglomerat” unit, “Nguba” cap carbonates and “Kundelungu” siliciclastic units. Analysis of faults interpreted from geophysical maps identified three major directions: E-W, NE-SW and NW-SE. The E-W faults are also interpreted as normal transfer faults such NE-SW structures, consistent with regional geological map. Although pole-dipole array of induced polarization (IP) survey was directly targeting disseminated Cu sulphide, its results suffer in responding to graphitic rocks and barren pyrite. Only relative small chargeable bodies need to be tested in drilling follow-up. A total of 15 targets have been generated through re-interpretation and integration of both geological mapping and remote sensing, geochemical and geophysical data, as well as existing drilling. Specific recommendations of follow-up works are advised for each type of target.
- Full Text:
Exploring the fertiliser potential of biosolids from algae integrated wastewater treatment systems
- Authors: Mlambo, Patricia Zanele
- Date: 2014
- Subjects: Sewage disposal plants , Sewage sludge as fertilizer , Algae -- Biotechnology , Sewage -- Purification -- Anaerobic treatment , Plant regulators , Biofertilizers , Microalgae -- Biotechnology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5957 , http://hdl.handle.net/10962/d1013342
- Description: High rate algae oxidation ponds (HRAOP) for domestic wastewater treatment generate biosolids that are predominantly microalgae. Consequently, HRAOP biosolids are enriched with minerals, amino acids, nutrients and possibly contain plant growth regulator (PGR)-like substances, which makes HRAOP biosolids attractive as fertiliser or PGR. This study investigated HRAOP biosolids as a starting material for a natural, cost-effective and readily-available eco-friendly organic fertiliser and/or PGRs. Various HRAOP extract formulations were prepared and their effect on plant growth and development was evaluated using selected bioassays. Initial screening included assessing the effect on change in specific leaf area, radish cotyledon expansion as an indicator of PGR-like activity, and seed germination index (GI). More detailed studies on fertiliser efficacy and PGR-like activity utilised bean (Phaseolus vulgaris) and tomato (Solanum lycopersicum) plants. Combined effects of sonicated (S) and 40% v/v methanol (M) extract (5:1 SM) had impressive plant responses, comparable to Hoagland solution (HS). Other potentially fertiliser formulations included 0.5% M, 1% M, 2.5% S and 5% S formulations. The 5:1 SM and 5% S showed greater PGR-like activity, promoting cotyledon expansion by 459 ± 0.02% and 362 ± 0.01%, respectively. GI data showed that none of the formulations negatively impacted germination. Further investigation showed that the 5% S formulation increased leaf length, width and area by 6.69 ± 0.24, 6.21 ± 0.2 mm and 41.55 ± 0.2 mm². All formulated fertiliser extracts had no adverse effect on chlorophyll content and plant nutrient balance as indicated by C:N (8-10:1) ratio. In addition, plants appeared to actively mobilise nutrients to regions where needed as evidenced by a shift in shoot: root ratio depending on C, N and water availability. Furthermore, 5% S caused a 75% increase in tomato productivity and had no effect on bean productivity. Whereas, 5:1 SM and 1% M formulation improved bean pod production by 33.3% and 11%, respectively but did not affect tomato production. Harvest index (HI) however indicated a 3% reduction in tomato productivity with 5:1 SM and little or no enhancement in bean productivity with both 5:1 SM and 5% S treatments. Bean plants treated with 5:1 SM and 5% S produced larger fruits, which could be an indication of the presence of a PGR effect. Overall, HRAOP biosolids extracts prepared and investigated in this study demonstrated both fertiliser characteristics and PGR-like activity with performances comparable and in some cases exceeding that of commercial products. However additional research is needed to confirm presence of PGR-like activities and fertiliser efficacy.
- Full Text:
- Authors: Mlambo, Patricia Zanele
- Date: 2014
- Subjects: Sewage disposal plants , Sewage sludge as fertilizer , Algae -- Biotechnology , Sewage -- Purification -- Anaerobic treatment , Plant regulators , Biofertilizers , Microalgae -- Biotechnology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5957 , http://hdl.handle.net/10962/d1013342
- Description: High rate algae oxidation ponds (HRAOP) for domestic wastewater treatment generate biosolids that are predominantly microalgae. Consequently, HRAOP biosolids are enriched with minerals, amino acids, nutrients and possibly contain plant growth regulator (PGR)-like substances, which makes HRAOP biosolids attractive as fertiliser or PGR. This study investigated HRAOP biosolids as a starting material for a natural, cost-effective and readily-available eco-friendly organic fertiliser and/or PGRs. Various HRAOP extract formulations were prepared and their effect on plant growth and development was evaluated using selected bioassays. Initial screening included assessing the effect on change in specific leaf area, radish cotyledon expansion as an indicator of PGR-like activity, and seed germination index (GI). More detailed studies on fertiliser efficacy and PGR-like activity utilised bean (Phaseolus vulgaris) and tomato (Solanum lycopersicum) plants. Combined effects of sonicated (S) and 40% v/v methanol (M) extract (5:1 SM) had impressive plant responses, comparable to Hoagland solution (HS). Other potentially fertiliser formulations included 0.5% M, 1% M, 2.5% S and 5% S formulations. The 5:1 SM and 5% S showed greater PGR-like activity, promoting cotyledon expansion by 459 ± 0.02% and 362 ± 0.01%, respectively. GI data showed that none of the formulations negatively impacted germination. Further investigation showed that the 5% S formulation increased leaf length, width and area by 6.69 ± 0.24, 6.21 ± 0.2 mm and 41.55 ± 0.2 mm². All formulated fertiliser extracts had no adverse effect on chlorophyll content and plant nutrient balance as indicated by C:N (8-10:1) ratio. In addition, plants appeared to actively mobilise nutrients to regions where needed as evidenced by a shift in shoot: root ratio depending on C, N and water availability. Furthermore, 5% S caused a 75% increase in tomato productivity and had no effect on bean productivity. Whereas, 5:1 SM and 1% M formulation improved bean pod production by 33.3% and 11%, respectively but did not affect tomato production. Harvest index (HI) however indicated a 3% reduction in tomato productivity with 5:1 SM and little or no enhancement in bean productivity with both 5:1 SM and 5% S treatments. Bean plants treated with 5:1 SM and 5% S produced larger fruits, which could be an indication of the presence of a PGR effect. Overall, HRAOP biosolids extracts prepared and investigated in this study demonstrated both fertiliser characteristics and PGR-like activity with performances comparable and in some cases exceeding that of commercial products. However additional research is needed to confirm presence of PGR-like activities and fertiliser efficacy.
- Full Text:
Fabrication, characterization and application of phthalocyanine-magnetite hybrid nanofibers
- Authors: Modisha, Phillimon Mokanne
- Date: 2014
- Subjects: Nanofibers , Nanoparticles , Magnetite
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4500 , http://hdl.handle.net/10962/d1013223
- Description: Magnetic nanoparticles comprising magnetite (Fe3O4) were functionalized with 3-aminopropyl-triethoxysilane forming amino functionalized magnetite nanoparticles (AMNPs). The amino group allows for conjugation with zinc octacarboxyphthalocyanine (ZnOCPc) or zinc tetracarboxyphthalocyanine (ZnTCPc) via the carboxyl group to form an amide bond. A reduced aggregation of ZnTCPc is observed after conjugation with AMNPs. The thermal stability, conjugation, morphology and the sizes of the nanoparticles and their conjugates were confirmed using thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and Powder X-ray diffractometry (PXRD), respectively. The covalent linkage of AMNPs to ZnOCPc or ZnTCPc resulted in improvement in the photophysical behavior of the phthalocyanines. Improvement in the triplet quantum yield (ΦT), singlet oxygen quantum yield (ΦΔ), triplet lifetime (τT) and singlet oxygen lifetime (τΔ) of the ZnOCPc or ZnTCPc were observed, hence improving the photosensitizers efficiency. The conjugates comprising of zinc octacarboxyphthalocyanine (ZnOCPc) and AMNPs were electrospun into fibers using polyamide-6 (PA-6). This was used for the photodegradation of Orange-G and compared with ZnOCPc-AMNPs in suspension. For ZnOCPc-AMNPs in suspension, it is noteworthy that the catalyst can be easily recovered using an external magnetic field. The singlet oxygen generation increases as we increase the fiber diameter by increasing the ZnOCPc concentration. The singlet oxygen quantum yield is higher for PA-6/ZnOCPc-AMNPs nanofibers when compared to PA-6/ZnOCPc. The rate of degradation of Orange-G increased with an increase in the singlet oxygen quantum yield. Moreover, the kinetic analysis showed that the photodecomposition of Orange-G is a first-order reaction according to the Langmuir-Hinshelwood model.
- Full Text:
- Authors: Modisha, Phillimon Mokanne
- Date: 2014
- Subjects: Nanofibers , Nanoparticles , Magnetite
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4500 , http://hdl.handle.net/10962/d1013223
- Description: Magnetic nanoparticles comprising magnetite (Fe3O4) were functionalized with 3-aminopropyl-triethoxysilane forming amino functionalized magnetite nanoparticles (AMNPs). The amino group allows for conjugation with zinc octacarboxyphthalocyanine (ZnOCPc) or zinc tetracarboxyphthalocyanine (ZnTCPc) via the carboxyl group to form an amide bond. A reduced aggregation of ZnTCPc is observed after conjugation with AMNPs. The thermal stability, conjugation, morphology and the sizes of the nanoparticles and their conjugates were confirmed using thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and Powder X-ray diffractometry (PXRD), respectively. The covalent linkage of AMNPs to ZnOCPc or ZnTCPc resulted in improvement in the photophysical behavior of the phthalocyanines. Improvement in the triplet quantum yield (ΦT), singlet oxygen quantum yield (ΦΔ), triplet lifetime (τT) and singlet oxygen lifetime (τΔ) of the ZnOCPc or ZnTCPc were observed, hence improving the photosensitizers efficiency. The conjugates comprising of zinc octacarboxyphthalocyanine (ZnOCPc) and AMNPs were electrospun into fibers using polyamide-6 (PA-6). This was used for the photodegradation of Orange-G and compared with ZnOCPc-AMNPs in suspension. For ZnOCPc-AMNPs in suspension, it is noteworthy that the catalyst can be easily recovered using an external magnetic field. The singlet oxygen generation increases as we increase the fiber diameter by increasing the ZnOCPc concentration. The singlet oxygen quantum yield is higher for PA-6/ZnOCPc-AMNPs nanofibers when compared to PA-6/ZnOCPc. The rate of degradation of Orange-G increased with an increase in the singlet oxygen quantum yield. Moreover, the kinetic analysis showed that the photodecomposition of Orange-G is a first-order reaction according to the Langmuir-Hinshelwood model.
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Factors influencing ecological sustainability in the ostrich industry in the Little Karoo, South Africa
- Authors: Wheeler, Anita
- Date: 2014
- Subjects: Ostrich farms -- South Africa -- Little Karoo , Land degradation -- South Africa -- Little Karoo , Land use -- South Africa -- Little Karoo , Land degradation -- Environmental aspects -- South Africa -- Little Karoo , Conservation of natural resources -- South Africa -- Little Karoo , Sustainable development -- South Africa -- Little Karoo , Sustainable agriculture -- South Africa -- Little Karoo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4259 , http://hdl.handle.net/10962/d1011741 , Ostrich farms -- South Africa -- Little Karoo , Land degradation -- South Africa -- Little Karoo , Land use -- South Africa -- Little Karoo , Land degradation -- Environmental aspects -- South Africa -- Little Karoo , Conservation of natural resources -- South Africa -- Little Karoo , Sustainable development -- South Africa -- Little Karoo , Sustainable agriculture -- South Africa -- Little Karoo
- Description: The Little Karoo, situated in the Succulent Karoo biome of South Africa, has been heavily transformed by land use, and only 8.6% of the remaining natural vegetation is considered to be intact. There is ample evidence that the main cause of degradation is the overstocking of ostriches, an industry that has been the major economic driver of the area for more than 150 years. The ostrich stocking rate currently recommended is 22 ha.ostrich⁻¹. A literature review was used to examine the evidence, assumptions and rationale on which recommended and actually implemented stocking rates for ostriches are based. No experimental evidence using ostriches was found that supported the recommended stocking rate as either ecologically or economically sustainable. From the literature, there appears to be a wide gap between what are considered to be economically and ecologically sustainable stocking rates, with the implication that these two aims may be impossible to reconcile when practicing ostrich farming on natural veld. A comparison of recommended with actual stocking rates among land managers in the Little Karoo showed that all land managers far exceeded the recommended agricultural stocking rate of 22.8 ha.ostrich⁻¹. However, the stocking rates reported by land managers to the South African Ostrich Business Chamber were found to accurately reflect actual numbers determined when whole flocks had to be slaughtered after an outbreak of avian influenza. The recommended stocking rate appeared to be irrelevant to ostrich flock breeders and there appeared to be a trade-off between profitable ostrich farming and sustainable land-use practices. This study also investigated the attitudes and behaviour of ostrich farmers. It was found that environmental attitude is most likely the most important characteristic of a land manager to ensure positive conservation behaviour. This characteristic was most prominent in younger land managers with larger farms. The last component of the study integrated the context and complexity of the long term social, economic and ecological sustainability of this industry through the development of a logic model. The results showed a general lack of linkages between industry elements which impact on achieving sustainability targets. Greater collaboration between industry role-players, organized agriculture and conservation organizations is required to find a balance between utilization and conservation in the ostrich industry.
- Full Text:
- Authors: Wheeler, Anita
- Date: 2014
- Subjects: Ostrich farms -- South Africa -- Little Karoo , Land degradation -- South Africa -- Little Karoo , Land use -- South Africa -- Little Karoo , Land degradation -- Environmental aspects -- South Africa -- Little Karoo , Conservation of natural resources -- South Africa -- Little Karoo , Sustainable development -- South Africa -- Little Karoo , Sustainable agriculture -- South Africa -- Little Karoo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4259 , http://hdl.handle.net/10962/d1011741 , Ostrich farms -- South Africa -- Little Karoo , Land degradation -- South Africa -- Little Karoo , Land use -- South Africa -- Little Karoo , Land degradation -- Environmental aspects -- South Africa -- Little Karoo , Conservation of natural resources -- South Africa -- Little Karoo , Sustainable development -- South Africa -- Little Karoo , Sustainable agriculture -- South Africa -- Little Karoo
- Description: The Little Karoo, situated in the Succulent Karoo biome of South Africa, has been heavily transformed by land use, and only 8.6% of the remaining natural vegetation is considered to be intact. There is ample evidence that the main cause of degradation is the overstocking of ostriches, an industry that has been the major economic driver of the area for more than 150 years. The ostrich stocking rate currently recommended is 22 ha.ostrich⁻¹. A literature review was used to examine the evidence, assumptions and rationale on which recommended and actually implemented stocking rates for ostriches are based. No experimental evidence using ostriches was found that supported the recommended stocking rate as either ecologically or economically sustainable. From the literature, there appears to be a wide gap between what are considered to be economically and ecologically sustainable stocking rates, with the implication that these two aims may be impossible to reconcile when practicing ostrich farming on natural veld. A comparison of recommended with actual stocking rates among land managers in the Little Karoo showed that all land managers far exceeded the recommended agricultural stocking rate of 22.8 ha.ostrich⁻¹. However, the stocking rates reported by land managers to the South African Ostrich Business Chamber were found to accurately reflect actual numbers determined when whole flocks had to be slaughtered after an outbreak of avian influenza. The recommended stocking rate appeared to be irrelevant to ostrich flock breeders and there appeared to be a trade-off between profitable ostrich farming and sustainable land-use practices. This study also investigated the attitudes and behaviour of ostrich farmers. It was found that environmental attitude is most likely the most important characteristic of a land manager to ensure positive conservation behaviour. This characteristic was most prominent in younger land managers with larger farms. The last component of the study integrated the context and complexity of the long term social, economic and ecological sustainability of this industry through the development of a logic model. The results showed a general lack of linkages between industry elements which impact on achieving sustainability targets. Greater collaboration between industry role-players, organized agriculture and conservation organizations is required to find a balance between utilization and conservation in the ostrich industry.
- Full Text:
Genesis of BIF-hosted hematite iron ore deposits in the central part of the Maremane anticline, Northern Cape Province, South Africa
- Authors: Land, Jarred
- Date: 2014
- Subjects: Hematite -- South Africa -- Northern Cape , Anticlines -- South Africa -- Northern Cape , Geology, Stratigraphic -- Proterozoic , Hydrothermal deposits -- Northern Cape , Rare earth metals -- Northern Cape , Iron ores -- Geology -- Northern Cape , Transvaal Supergroup (South Africa)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5095 , http://hdl.handle.net/10962/d1020905
- Description: The Paleoproterozoic Transvaal Supergroup in the Northern Cape Province of South Africa is host to high-grade BIF-hosted hematite iron-ore deposits and is the country’s most important source of iron to date. Previous work has failed to provide a robust and all-inclusive genetic model for such deposits in the Transvaal Supergroup; in particular, the role of hydrothermal processes in ore-genesis has not been adequately clarified. Recent studies by the author have produced evidence for hydrothermal alteration in shales (Olifantshoek Supergroup) stratigraphically overlying the iron-ore intervals; this has highlighted the need to reassess current ore-forming models which place residual supergene processes at the core of oregenesis. This thesis focuses on providing new insights into the processes responsible for the genesis of hematite iron ores in the Maremane anticline through the use of newly available exploration drill-core material from the centre of the anticline. The study involved standard mineralogical investigations using transmitted/reflected light microscopy as well as instrumental techniques (XRD, EPMA); and the employment of traditional whole-rock geochemical analysis on samples collected from two boreholes drilled in the centre of the Maremane anticline, Northern Cape Province. Rare earth element analysis (via ICP-MS) and oxygen isotope data from hematite separates complement the whole-rock data. Iron-ore mineralisation examined in this thesis is typified by the dominance of Fe-oxide (as hematite), which reaches whole-rock abundances of up to 98 wt. % Fe₂O₃. Textural and whole-rock geochemical variations in the ores likely reflect a variable protolith, from BIF to Fe-bearing shale. A standard supergene model invoking immobility and residual enrichment of iron is called into question on the basis of the relative degrees of enrichment recorded in the ores with respect to other, traditionally immobile elements during chemical weathering, such as Al₂O₃ and TiO₂. Furthermore, the apparently conservative behaviour of REE in the Fe ore (i.e. low-grade and high-grade iron ore) further emphasises the variable protolith theory. Hydrothermally-induced ferruginisation is suggested to post-date the deposition of the post-Transvaal Olifantshoek shales, and is likely to be linked to a sub-surface transgressive hydrothermal event which indiscriminately transforms both shale and BIF into Fe-ore. A revised, hydrothermal model for the formation of BIF-hosted high-grade hematite iron ore deposits in the central part of the Maremane anticline is proposed, and some ideas of the author for further follow-up research are presented.
- Full Text:
- Authors: Land, Jarred
- Date: 2014
- Subjects: Hematite -- South Africa -- Northern Cape , Anticlines -- South Africa -- Northern Cape , Geology, Stratigraphic -- Proterozoic , Hydrothermal deposits -- Northern Cape , Rare earth metals -- Northern Cape , Iron ores -- Geology -- Northern Cape , Transvaal Supergroup (South Africa)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5095 , http://hdl.handle.net/10962/d1020905
- Description: The Paleoproterozoic Transvaal Supergroup in the Northern Cape Province of South Africa is host to high-grade BIF-hosted hematite iron-ore deposits and is the country’s most important source of iron to date. Previous work has failed to provide a robust and all-inclusive genetic model for such deposits in the Transvaal Supergroup; in particular, the role of hydrothermal processes in ore-genesis has not been adequately clarified. Recent studies by the author have produced evidence for hydrothermal alteration in shales (Olifantshoek Supergroup) stratigraphically overlying the iron-ore intervals; this has highlighted the need to reassess current ore-forming models which place residual supergene processes at the core of oregenesis. This thesis focuses on providing new insights into the processes responsible for the genesis of hematite iron ores in the Maremane anticline through the use of newly available exploration drill-core material from the centre of the anticline. The study involved standard mineralogical investigations using transmitted/reflected light microscopy as well as instrumental techniques (XRD, EPMA); and the employment of traditional whole-rock geochemical analysis on samples collected from two boreholes drilled in the centre of the Maremane anticline, Northern Cape Province. Rare earth element analysis (via ICP-MS) and oxygen isotope data from hematite separates complement the whole-rock data. Iron-ore mineralisation examined in this thesis is typified by the dominance of Fe-oxide (as hematite), which reaches whole-rock abundances of up to 98 wt. % Fe₂O₃. Textural and whole-rock geochemical variations in the ores likely reflect a variable protolith, from BIF to Fe-bearing shale. A standard supergene model invoking immobility and residual enrichment of iron is called into question on the basis of the relative degrees of enrichment recorded in the ores with respect to other, traditionally immobile elements during chemical weathering, such as Al₂O₃ and TiO₂. Furthermore, the apparently conservative behaviour of REE in the Fe ore (i.e. low-grade and high-grade iron ore) further emphasises the variable protolith theory. Hydrothermally-induced ferruginisation is suggested to post-date the deposition of the post-Transvaal Olifantshoek shales, and is likely to be linked to a sub-surface transgressive hydrothermal event which indiscriminately transforms both shale and BIF into Fe-ore. A revised, hydrothermal model for the formation of BIF-hosted high-grade hematite iron ore deposits in the central part of the Maremane anticline is proposed, and some ideas of the author for further follow-up research are presented.
- Full Text:
Genesis of karst-hosted manganese ores of the Postmasburg Manganese Field, South Africa with emphasis on evidence for hydrothermal processes
- Authors: Fairey, Brenton John
- Date: 2014
- Subjects: Karst -- South Africa -- Postmasburg , Manganese ores -- South Africa -- Postmasburg , Hydrothermal alteration -- South Africa -- Postmasburg , Manganese mines and mining -- South Africa -- Northern Cape , Petrology , Mineralogical chemistry , Geochemistry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5094 , http://hdl.handle.net/10962/d1020904
- Description: The Postmasburg Manganese Field (PMF), located in the Northern Cape Province of South Africa, once represented one of the largest sources of manganese ore worldwide. However, the discovery of the giant manganese deposits of the Kalahari Manganese Field (KMF) led to the gradual decline in manganese mining activity in the PMF. Two belts of manganese ore deposits have been distinguished in the PMF, namely the Western Belt of ferruginous manganese ores and the Eastern Belt of siliceous manganese ores. Prevailing models of ore formation in these two belts invoke karstification of manganese-rich dolomites and residual accumulation of manganese wad which later underwent diagenetic and low-grade metamorphic processes. For the most part, the role of hydrothermal processes in ore formation and metasomatic alteration is not addressed. The identification of an abundance of common and some rare Al-, Na-, K- and Ba-bearing minerals, particularly aegirine, albite, microcline, banalsite, sérandite-pectolite, paragonite and natrolite in the PMF ores studied in this thesis, is indicative of the influence of hydrothermal activity. Enrichments in Na, K and/or Ba in the ores are generally on a percentage level for the majority of samples analysed through bulk-rock techniques. The discovery of a Ba-Mn arsenate/vanadate similar to gamagarite may also indicate that the hydrothermal fluid affecting the ores was not only alkali-rich but also probably contained some As and V. The fluid was likely to be oxidized and alkaline in nature and is thought to have been a mature basinal brine. Various replacement textures, particularly of Na- and Krich minerals by Ba-bearing phases, suggest sequential deposition of gangue as well as oreminerals from the hydrothermal fluid, with Ba phases being deposited at a later stage. The stratigraphic variability of the studied ores and the deviation of their character from the pigeon-hole-type classification of ferruginous and siliceous ores in the literature, suggests that a re-evaluation of genetic models is warranted. The discovery of hydrothermallydeposited alkali-rich assemblages in the PMF and KMF provides grounding for further investigation into a possible regional-scale hydrothermal event at least re-constituting the ores. Some shortcomings in previous works include disregard for the highly variable nature of the PMF deposits, the effects of hydrothermal activity of the ores and the existence of stratigraphic discrepancies. This study provides a single, broad model for the development of all manganese deposits of the PMF. The source of metals is attributed to all formations that stratigraphically overly the Reivilo Formation of the Campbellrand Subgroup (including the Reivilo Formation itself). The main process by which metals are accumulated is attributed to karstification of the dolomites. The interaction of oxidized, alkaline brines with the ores is considered and the overlying Asbestos Hills Subgroup BIF is suggested as a potential source of alkali metals.
- Full Text:
- Authors: Fairey, Brenton John
- Date: 2014
- Subjects: Karst -- South Africa -- Postmasburg , Manganese ores -- South Africa -- Postmasburg , Hydrothermal alteration -- South Africa -- Postmasburg , Manganese mines and mining -- South Africa -- Northern Cape , Petrology , Mineralogical chemistry , Geochemistry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5094 , http://hdl.handle.net/10962/d1020904
- Description: The Postmasburg Manganese Field (PMF), located in the Northern Cape Province of South Africa, once represented one of the largest sources of manganese ore worldwide. However, the discovery of the giant manganese deposits of the Kalahari Manganese Field (KMF) led to the gradual decline in manganese mining activity in the PMF. Two belts of manganese ore deposits have been distinguished in the PMF, namely the Western Belt of ferruginous manganese ores and the Eastern Belt of siliceous manganese ores. Prevailing models of ore formation in these two belts invoke karstification of manganese-rich dolomites and residual accumulation of manganese wad which later underwent diagenetic and low-grade metamorphic processes. For the most part, the role of hydrothermal processes in ore formation and metasomatic alteration is not addressed. The identification of an abundance of common and some rare Al-, Na-, K- and Ba-bearing minerals, particularly aegirine, albite, microcline, banalsite, sérandite-pectolite, paragonite and natrolite in the PMF ores studied in this thesis, is indicative of the influence of hydrothermal activity. Enrichments in Na, K and/or Ba in the ores are generally on a percentage level for the majority of samples analysed through bulk-rock techniques. The discovery of a Ba-Mn arsenate/vanadate similar to gamagarite may also indicate that the hydrothermal fluid affecting the ores was not only alkali-rich but also probably contained some As and V. The fluid was likely to be oxidized and alkaline in nature and is thought to have been a mature basinal brine. Various replacement textures, particularly of Na- and Krich minerals by Ba-bearing phases, suggest sequential deposition of gangue as well as oreminerals from the hydrothermal fluid, with Ba phases being deposited at a later stage. The stratigraphic variability of the studied ores and the deviation of their character from the pigeon-hole-type classification of ferruginous and siliceous ores in the literature, suggests that a re-evaluation of genetic models is warranted. The discovery of hydrothermallydeposited alkali-rich assemblages in the PMF and KMF provides grounding for further investigation into a possible regional-scale hydrothermal event at least re-constituting the ores. Some shortcomings in previous works include disregard for the highly variable nature of the PMF deposits, the effects of hydrothermal activity of the ores and the existence of stratigraphic discrepancies. This study provides a single, broad model for the development of all manganese deposits of the PMF. The source of metals is attributed to all formations that stratigraphically overly the Reivilo Formation of the Campbellrand Subgroup (including the Reivilo Formation itself). The main process by which metals are accumulated is attributed to karstification of the dolomites. The interaction of oxidized, alkaline brines with the ores is considered and the overlying Asbestos Hills Subgroup BIF is suggested as a potential source of alkali metals.
- Full Text:
Genetic and biological characterisation of a novel South African Plutella xylostella granulovirus (PlxyGV) isolate
- Authors: Abdulkadir, Fatima
- Date: 2014
- Subjects: Diamondback moth , Diamondback moth -- Control -- South Africa , Plutellidae -- Control -- South Africa , Baculoviruses , Cruciferae -- Diseases and pests -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4113 , http://hdl.handle.net/10962/d1013059
- Description: The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is an important pest of cruciferous crops worldwide. The prolonged use of synthetic chemical insecticides as a primary means of control has resulted in the development of resistance in pest populations. In addition, the pest has also evolved resistance to the bacterial insecticidal protein of Bacillus thuringiensis which is also widely used as a method of control. Baculoviruses are considered as effective alternatives to conventional methods of control when incorporated into integrated pest management (IPM) programmes. These viruses target the larval stages of insects, are generally host-specific and are safe for use in the environment. This study aimed to isolate a baculovirus from a laboratory-reared P. xylostella colony, characterise it genetically and then evaluate its virulence against neonate and fourth instar larvae. A laboratory colony of P. xylostella was established using pupae and asymptomatic larvae collected from a cabbage plantation outside Grahamstown in the Eastern Cape province of South Africa. The colony flourished in the laboratory due to prime conditions and availability of food. The duration of development from egg to adult was determined by observation and imaging of the various life stages. The mean developmental time from egg to adult was observed to be 14.59 ± 0.21 days. The population of the insects increased rapidly in number leading to overcrowding of the insect colony, and hence appearance of larvae with viral symptoms. Occlusion bodies (OBs) were extracted from symptomatic larval cadavers and purified by glycerol gradient centrifugation. Analysis of the purified OBs by transmission electron microscopy revealed the presence of a granulovirus which was named PlxyGV-SA. The virus isolate was genetically characterised by restriction endonuclease analysis of the genomic DNA, and PCR amplification and sequencing of selected viral genes. The complete genome sequence of a Japanese P. xylostella granulovirus isolate, PlxyGV-Japan, has been deposited on the GenBank database providing a reference strain for comparison with DNA profiles and selected gene sequences of PlxyGV-SA. BLAST analysis of the granulin gene confirmed the isolation of a novel South African PlxyGV isolate. Comparison of the restriction profiles of PlxyGV-SA with profiles of PlxyGV-Japan and other documented PlxyGV profiles obtained by agarose gel electrophoresis revealed that PlxyGV-SA is a genetically distinct isolate. The data obtained from the sequencing and alignment of ecdysteroid UDP-glucosyltransferase (egt), late expression factor 8 (lef-8) and late expression factor 9 (lef-9) genes with those of PlxyGV-Japan also showed that PlxyGV-SA is a genetically different isolate. In order to determine the biological activity of PlxyGV-SA against neonate and fourth instar P. xylostella larvae, surface dose bioassays were conducted. The median lethal concentration of the virus required to kill 50% (LC₅₀) and 90% (LC₉₀) of the larvae was estimated by feeding insects with a range of doses. In addition, the time to kill 50% of the larvae (LT₅₀) was determined by feeding insects with the LC₉₀ concentration. Larval mortality was monitored daily until pupation. The data obtained from the dose response assays were subjected to probit analysis using Proban statistical software. The time response was determined using GraphPad Prism software (version 6.0). The LC₅₀ and LC₉₀ values for the neonate larvae were 3.56 × 10⁵ and 1.14 × 10⁷ OBs/ml respectively. The LT₅₀ was determined to be 104 hours. The neonate larvae were found to be more susceptible to infection than the fourth instar larvae with the same virus concentration. The concentrations used for the neonate larvae assay did not have a significant effect on the fourth instar as no mortality was recorded. This is the first study to describe a novel South African PlxyGV isolate and the results suggest that PlxyGV-SA has significant potential for development as an effective biopesticide for the control of P. xylostella in the field.
- Full Text:
- Authors: Abdulkadir, Fatima
- Date: 2014
- Subjects: Diamondback moth , Diamondback moth -- Control -- South Africa , Plutellidae -- Control -- South Africa , Baculoviruses , Cruciferae -- Diseases and pests -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4113 , http://hdl.handle.net/10962/d1013059
- Description: The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is an important pest of cruciferous crops worldwide. The prolonged use of synthetic chemical insecticides as a primary means of control has resulted in the development of resistance in pest populations. In addition, the pest has also evolved resistance to the bacterial insecticidal protein of Bacillus thuringiensis which is also widely used as a method of control. Baculoviruses are considered as effective alternatives to conventional methods of control when incorporated into integrated pest management (IPM) programmes. These viruses target the larval stages of insects, are generally host-specific and are safe for use in the environment. This study aimed to isolate a baculovirus from a laboratory-reared P. xylostella colony, characterise it genetically and then evaluate its virulence against neonate and fourth instar larvae. A laboratory colony of P. xylostella was established using pupae and asymptomatic larvae collected from a cabbage plantation outside Grahamstown in the Eastern Cape province of South Africa. The colony flourished in the laboratory due to prime conditions and availability of food. The duration of development from egg to adult was determined by observation and imaging of the various life stages. The mean developmental time from egg to adult was observed to be 14.59 ± 0.21 days. The population of the insects increased rapidly in number leading to overcrowding of the insect colony, and hence appearance of larvae with viral symptoms. Occlusion bodies (OBs) were extracted from symptomatic larval cadavers and purified by glycerol gradient centrifugation. Analysis of the purified OBs by transmission electron microscopy revealed the presence of a granulovirus which was named PlxyGV-SA. The virus isolate was genetically characterised by restriction endonuclease analysis of the genomic DNA, and PCR amplification and sequencing of selected viral genes. The complete genome sequence of a Japanese P. xylostella granulovirus isolate, PlxyGV-Japan, has been deposited on the GenBank database providing a reference strain for comparison with DNA profiles and selected gene sequences of PlxyGV-SA. BLAST analysis of the granulin gene confirmed the isolation of a novel South African PlxyGV isolate. Comparison of the restriction profiles of PlxyGV-SA with profiles of PlxyGV-Japan and other documented PlxyGV profiles obtained by agarose gel electrophoresis revealed that PlxyGV-SA is a genetically distinct isolate. The data obtained from the sequencing and alignment of ecdysteroid UDP-glucosyltransferase (egt), late expression factor 8 (lef-8) and late expression factor 9 (lef-9) genes with those of PlxyGV-Japan also showed that PlxyGV-SA is a genetically different isolate. In order to determine the biological activity of PlxyGV-SA against neonate and fourth instar P. xylostella larvae, surface dose bioassays were conducted. The median lethal concentration of the virus required to kill 50% (LC₅₀) and 90% (LC₉₀) of the larvae was estimated by feeding insects with a range of doses. In addition, the time to kill 50% of the larvae (LT₅₀) was determined by feeding insects with the LC₉₀ concentration. Larval mortality was monitored daily until pupation. The data obtained from the dose response assays were subjected to probit analysis using Proban statistical software. The time response was determined using GraphPad Prism software (version 6.0). The LC₅₀ and LC₉₀ values for the neonate larvae were 3.56 × 10⁵ and 1.14 × 10⁷ OBs/ml respectively. The LT₅₀ was determined to be 104 hours. The neonate larvae were found to be more susceptible to infection than the fourth instar larvae with the same virus concentration. The concentrations used for the neonate larvae assay did not have a significant effect on the fourth instar as no mortality was recorded. This is the first study to describe a novel South African PlxyGV isolate and the results suggest that PlxyGV-SA has significant potential for development as an effective biopesticide for the control of P. xylostella in the field.
- Full Text:
Genetic and morphometric variation of Octopus vulgaris in the Benguela Current region
- Authors: De Beer, Chénelle Lesley
- Date: 2014
- Subjects: Common octopus -- Africa, Southern , Common octopus -- Genetics , Common octopus -- Morphology , Common octopus -- Geographical distribution , Variation (Biology) , Benguela Current
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5362 , http://hdl.handle.net/10962/d1012971
- Description: The Benguela Current is a cold eastern boundary current located on the south-western coast of the African continent. The establishment of its present day features approximately two million years ago has triggered allopatric events which have driven genetic and/or phenotypic differentiation in many of the warm-temperate organisms that previously had continuous distributions along the south and west coast of southern Africa. However, since many of these species have responded differently, despite similar isolation times, research in this region provides a unique opportunity to increase our understanding of evolutionary processes. The common octopus (Octopus vulgaris, Cuvier 1797) is a coastal, sedentary species, inhabiting coral reefs or rocky environments at depths of up to a 100m. It is considered to be one of the most extensively studied cephalopod species due to its worldwide distribution. However, very little research has been conducted on O. vulgaris in southern Africa. In order to gain a holistic understanding of the effects of the Benguela Current on population connectivity, genetic and phenotypic diversity, and evolutionary history of O. vulgaris, a comparative genetic and morphological study was conducted across the Benguela region. A total of 168 specimens of O. vulgaris were collected from four different regions across the Benguela system. A small tissue sample was preserved in ethanol for molecular analysis, and the specimen was frozen whole for morphometric analysis in the laboratory. Octopus vulgaris genetic population structure and evolutionary history was investigated using a 580bp fragment of the mitochondrial cytochrome b (cytb) gene for 76 individuals located within the Benguela region, yielding 10 different haplotypes. AMOVA and pairwise FST analyses revealed significant genetic differentiation suggesting a northern-southern Benguela divergence. Estimates of time since most recent common ancestor, based on biogeographical calibrators and coalescent analyses, indicated that isolation between the Angolan and South African population occurred between ~231 Ka and 1 Ma. Mismatch distribution analyses revealed a past population expansion within the South African O. vulgaris roughly 129.31 Ka, whilst Bayesian skyline plots were indicative of gradual demographic growth within the Angolan population in the last ~100 Ka. Observed O. vulgaris population structure and demographic history was likely the result of historical climate-induced change within the system. Reconstruction of phylogenetic relationships within the Octopus genus, using cytb and COI suggest that O. vulgaris is not a monophyletic group and a major systematic revision is required. Furthermore, unidentified individuals from South Africa were found to group with species from Indo-West Pacific Oceans and were therefore considered to have been translocated through ballast water from Asia. While the molecular analysis indicated a significant northern-southern Benguela structure results from the principle component analysis (PCA) and discriminate function analysis (DFA) were unable to distinguish between O. vulgaris from different sampling localities throughout the Benguela Current region based on soft-parts, hard-parts and meristic characters. The lack of phenotypic variation, despite significant genetic divergence, highlights the importance of multi-method approaches in gaining a holistic understanding of the taxonomy and biogeography of species.
- Full Text:
- Authors: De Beer, Chénelle Lesley
- Date: 2014
- Subjects: Common octopus -- Africa, Southern , Common octopus -- Genetics , Common octopus -- Morphology , Common octopus -- Geographical distribution , Variation (Biology) , Benguela Current
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5362 , http://hdl.handle.net/10962/d1012971
- Description: The Benguela Current is a cold eastern boundary current located on the south-western coast of the African continent. The establishment of its present day features approximately two million years ago has triggered allopatric events which have driven genetic and/or phenotypic differentiation in many of the warm-temperate organisms that previously had continuous distributions along the south and west coast of southern Africa. However, since many of these species have responded differently, despite similar isolation times, research in this region provides a unique opportunity to increase our understanding of evolutionary processes. The common octopus (Octopus vulgaris, Cuvier 1797) is a coastal, sedentary species, inhabiting coral reefs or rocky environments at depths of up to a 100m. It is considered to be one of the most extensively studied cephalopod species due to its worldwide distribution. However, very little research has been conducted on O. vulgaris in southern Africa. In order to gain a holistic understanding of the effects of the Benguela Current on population connectivity, genetic and phenotypic diversity, and evolutionary history of O. vulgaris, a comparative genetic and morphological study was conducted across the Benguela region. A total of 168 specimens of O. vulgaris were collected from four different regions across the Benguela system. A small tissue sample was preserved in ethanol for molecular analysis, and the specimen was frozen whole for morphometric analysis in the laboratory. Octopus vulgaris genetic population structure and evolutionary history was investigated using a 580bp fragment of the mitochondrial cytochrome b (cytb) gene for 76 individuals located within the Benguela region, yielding 10 different haplotypes. AMOVA and pairwise FST analyses revealed significant genetic differentiation suggesting a northern-southern Benguela divergence. Estimates of time since most recent common ancestor, based on biogeographical calibrators and coalescent analyses, indicated that isolation between the Angolan and South African population occurred between ~231 Ka and 1 Ma. Mismatch distribution analyses revealed a past population expansion within the South African O. vulgaris roughly 129.31 Ka, whilst Bayesian skyline plots were indicative of gradual demographic growth within the Angolan population in the last ~100 Ka. Observed O. vulgaris population structure and demographic history was likely the result of historical climate-induced change within the system. Reconstruction of phylogenetic relationships within the Octopus genus, using cytb and COI suggest that O. vulgaris is not a monophyletic group and a major systematic revision is required. Furthermore, unidentified individuals from South Africa were found to group with species from Indo-West Pacific Oceans and were therefore considered to have been translocated through ballast water from Asia. While the molecular analysis indicated a significant northern-southern Benguela structure results from the principle component analysis (PCA) and discriminate function analysis (DFA) were unable to distinguish between O. vulgaris from different sampling localities throughout the Benguela Current region based on soft-parts, hard-parts and meristic characters. The lack of phenotypic variation, despite significant genetic divergence, highlights the importance of multi-method approaches in gaining a holistic understanding of the taxonomy and biogeography of species.
- Full Text:
Geographical variation in effects of nutrient levels and grazing intensity on community structure between upwelling and non-upwelling regions of South Africa
- Authors: Steele, Nikita
- Date: 2014
- Subjects: Upwelling (Oceanography) , Upwelling (Oceanography) -- Indian Ocean , Algae -- Indian Ocean , Algae -- Effect of grazing on -- Indian Ocean
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5873 , http://hdl.handle.net/10962/d1013013
- Description: The aim of this thesis was to assess the influence of upwelling on alga-grazer interactions in rocky shore communities along the south coast of South Africa using grazer exclusion treatments with controls and procedural controls set out in a block design and monitored for algal cover roughly monthly for one year. In the first experiment grazers were excluded from treatment plots at two upwelling and two non-upwelling sites and the rates of algal biomass accumulation were then compared. The upwelling sites showed significantly faster algal colonisation rates, with Ulva rigida being the first species to colonise the rocks. Final algal cover and biomass did not differ significantly between upwelling and non-upwelling sites in control plots open to grazers, but were significantly higher in grazer exclusion plots at upwelling sites indicating stronger grazing effects. This was confirmed by estimating the intensity of grazing using the log-response ratio (LRR), which was calculated from treatment and control plots. Upwelling sites had significantly lower LLR values indicating stronger grazing effects, than at non-upwelling sites, despite no difference in grazer abundances. The second experiment examined the effects of nutrient addition on algal growth and community composition by comparing high nutrient enrichment plots with low enrichment plots at one upwelling and one non-upwelling site. ANOVA indicated faster growth rates and significantly higher final algal biomass in high enrichment plots compared to low enrichment and control plots at both upwelling and non-upwelling sites. A two-way ANOVA indicated significantly higher algal cover in high enrichment plots compared to the data from the grazer exclusion plots in experiment 1 at both sites, suggesting that nutrient addition plays a major role in algal growth and community composition. The findings of these studies have shown significant differences between treatments, sites and seasons, with significant differences not only occurring in algal cover but also accumulation of algal biomass and recruitment patterns between treatments. The small scale local processes acting within a few centimetres (plots) or tens of meters (among blocks) can also be reflected over larger scales such as sites (upwelling/non-upwelling shores). Further, these studies have demonstrated that various factors such as the effects from increased nutrients at upwelling cells and the change in grazing effects due to enhanced nutrients can determine the abundance and diversity of the community structure, including an increase in the abundance of the fast growing algae Ulva rigida, and a slow recovery of the brown and red algae.
- Full Text:
- Authors: Steele, Nikita
- Date: 2014
- Subjects: Upwelling (Oceanography) , Upwelling (Oceanography) -- Indian Ocean , Algae -- Indian Ocean , Algae -- Effect of grazing on -- Indian Ocean
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5873 , http://hdl.handle.net/10962/d1013013
- Description: The aim of this thesis was to assess the influence of upwelling on alga-grazer interactions in rocky shore communities along the south coast of South Africa using grazer exclusion treatments with controls and procedural controls set out in a block design and monitored for algal cover roughly monthly for one year. In the first experiment grazers were excluded from treatment plots at two upwelling and two non-upwelling sites and the rates of algal biomass accumulation were then compared. The upwelling sites showed significantly faster algal colonisation rates, with Ulva rigida being the first species to colonise the rocks. Final algal cover and biomass did not differ significantly between upwelling and non-upwelling sites in control plots open to grazers, but were significantly higher in grazer exclusion plots at upwelling sites indicating stronger grazing effects. This was confirmed by estimating the intensity of grazing using the log-response ratio (LRR), which was calculated from treatment and control plots. Upwelling sites had significantly lower LLR values indicating stronger grazing effects, than at non-upwelling sites, despite no difference in grazer abundances. The second experiment examined the effects of nutrient addition on algal growth and community composition by comparing high nutrient enrichment plots with low enrichment plots at one upwelling and one non-upwelling site. ANOVA indicated faster growth rates and significantly higher final algal biomass in high enrichment plots compared to low enrichment and control plots at both upwelling and non-upwelling sites. A two-way ANOVA indicated significantly higher algal cover in high enrichment plots compared to the data from the grazer exclusion plots in experiment 1 at both sites, suggesting that nutrient addition plays a major role in algal growth and community composition. The findings of these studies have shown significant differences between treatments, sites and seasons, with significant differences not only occurring in algal cover but also accumulation of algal biomass and recruitment patterns between treatments. The small scale local processes acting within a few centimetres (plots) or tens of meters (among blocks) can also be reflected over larger scales such as sites (upwelling/non-upwelling shores). Further, these studies have demonstrated that various factors such as the effects from increased nutrients at upwelling cells and the change in grazing effects due to enhanced nutrients can determine the abundance and diversity of the community structure, including an increase in the abundance of the fast growing algae Ulva rigida, and a slow recovery of the brown and red algae.
- Full Text:
Geomorphic origin and dynamics of deep, peat-filled, valley bottom wetlands dominated by palmiet (Prionium serratum) : a case study based on the Goukou Wetland, Western Cape
- Authors: Job, Nancy Merle
- Date: 2014
- Subjects: Wetlands -- South Africa -- Western Cape , Peatlands -- South Africa -- Western Cape , Peatland conservation -- South Africa -- Western Cape , Peatland management -- South Africa , Aquatic plants -- South Africa -- Western Cape , Geomorphology -- South Africa -- Western Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4782 , http://hdl.handle.net/10962/d1013122
- Description: The Goukou Wetland is a 700 ha unchannelled valley bottom wetland near the town of Riversdale in the Western Cape of South Africa. The wetland is approximately 16 km long and between 200 and 800 m wide, with peat deposits up to 8 m deep that get progressively shallower downstream. The Goukou Wetland is one of the last remaining intact peatlands of significant size in the Western Cape. However, there is increasing human pressure on these peat wetlands, where the dominant plant is palmiet (Prionium serratum), which is endemic to the Western and Eastern Cape Provinces of South Africa. Palmiet is viewed as a problem plant by farmers as it is believed to block waterways and promote inundation of arable land and infrastructure. Many landowners therefore actively remove palmiet from peatlands, threatening the integrity of these wetlands. Although the hydrogeomorphic origin of large, non-peat floodplain and valley bottom wetlands has been investigated in South Africa, unchannelled valley-bottom wetlands with deep peat accumulations are rare features and have not been well studied. The hydrogeomorphic factors leading to peat accumulation have been documented elsewhere in Southern Africa, where aggradation due to sedimentation along trunk streams may block a tributary stream, elevating the local base level of the tributary, creating the accommodation space for organic sedimentation. Alternatively, sedimentation along a trunk stream at the toe of a tributary stream may similarly block a trunk stream, promoting organic sedimentation along the trunk stream upstream of the tributary. This pattern of peat accumulation is associated with declining peat thickness upstream of the blocked valley. In the case of the Goukou Wetland, however, peat depth and organic content was found to increase consistently upstream from the toe to the head of the wetland. The Goukou Wetland was graded along its length, with gradient increasing consistently upstream in response to longitudinal variation in discharge. There was no clear relationship between peat formation and tributary streams blocking the wetland. Instead, the distribution of peat and the extent of the wetland appeared to be controlled by the plant palmiet, whose clonal nature and robust root, rhizome and stem system allowed it to grow from channel banks and islands into fast-flowing river channels, slowing river flows and ultimately blocking the channel. The promotion of diffuse flows within the dense, monospecific stands of palmiet creates conditions conducive to water retention and peat accumulation. By growing across the full width of the valley floor, the plant is able to constrict the stream, trapping sediment and slowing flows such that the fluvial environment is changed from a fast flowing stream to one with slow, diffuse flow. These processes appear to lead to the formation of organic sediment, accumulating to form a deep peat basin. The sustained input of water from the folded and fractured quartzite lithologies of the Cape Supergroup that make up the Langeberg Mountains, which provide the bulk of the water supply to the wetland, is also important in promoting permanent flooding in the wetland. A feature that characterized the wetland was the fact that bedrock across the valley beneath the peat deposits exhibited a remarkably uniform elevation. This suggests that over long periods of time (tens to hundreds of thousands of years), bedrock has been laterally planed across the valley floor. It is proposed that valley widening associated with lateral planning of Uitenhage Formation rocks has taken place during periods of episodic very high flows. During these episodes, erosion cuts into the peat wetland and valley sides, cutting to bedrock and planing the valley floor to a uniform elevation for a given distance from the head of the wetland. Periods of episodic degradation are followed by periods of renewed peat accumulation associated with palmiet establishment, such that the wetland valley is shaped by repeated cycles of cutting and filling. Palmiet can be considered an “ecosystem engineer” that is integral to the formation of these deep peat basins. Removal of palmiet from these systems is likely to have negative consequences for the wetland and its functions in that water storage will be reduced, erosion will increase dramatically, and the water-purification function of the wetlands will be lost. Management of these wetlands, which are close to the geomorphic threshold slopes for their size, is therefore essential if they are to be preserved for the benefit of human well-being.
- Full Text:
- Authors: Job, Nancy Merle
- Date: 2014
- Subjects: Wetlands -- South Africa -- Western Cape , Peatlands -- South Africa -- Western Cape , Peatland conservation -- South Africa -- Western Cape , Peatland management -- South Africa , Aquatic plants -- South Africa -- Western Cape , Geomorphology -- South Africa -- Western Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4782 , http://hdl.handle.net/10962/d1013122
- Description: The Goukou Wetland is a 700 ha unchannelled valley bottom wetland near the town of Riversdale in the Western Cape of South Africa. The wetland is approximately 16 km long and between 200 and 800 m wide, with peat deposits up to 8 m deep that get progressively shallower downstream. The Goukou Wetland is one of the last remaining intact peatlands of significant size in the Western Cape. However, there is increasing human pressure on these peat wetlands, where the dominant plant is palmiet (Prionium serratum), which is endemic to the Western and Eastern Cape Provinces of South Africa. Palmiet is viewed as a problem plant by farmers as it is believed to block waterways and promote inundation of arable land and infrastructure. Many landowners therefore actively remove palmiet from peatlands, threatening the integrity of these wetlands. Although the hydrogeomorphic origin of large, non-peat floodplain and valley bottom wetlands has been investigated in South Africa, unchannelled valley-bottom wetlands with deep peat accumulations are rare features and have not been well studied. The hydrogeomorphic factors leading to peat accumulation have been documented elsewhere in Southern Africa, where aggradation due to sedimentation along trunk streams may block a tributary stream, elevating the local base level of the tributary, creating the accommodation space for organic sedimentation. Alternatively, sedimentation along a trunk stream at the toe of a tributary stream may similarly block a trunk stream, promoting organic sedimentation along the trunk stream upstream of the tributary. This pattern of peat accumulation is associated with declining peat thickness upstream of the blocked valley. In the case of the Goukou Wetland, however, peat depth and organic content was found to increase consistently upstream from the toe to the head of the wetland. The Goukou Wetland was graded along its length, with gradient increasing consistently upstream in response to longitudinal variation in discharge. There was no clear relationship between peat formation and tributary streams blocking the wetland. Instead, the distribution of peat and the extent of the wetland appeared to be controlled by the plant palmiet, whose clonal nature and robust root, rhizome and stem system allowed it to grow from channel banks and islands into fast-flowing river channels, slowing river flows and ultimately blocking the channel. The promotion of diffuse flows within the dense, monospecific stands of palmiet creates conditions conducive to water retention and peat accumulation. By growing across the full width of the valley floor, the plant is able to constrict the stream, trapping sediment and slowing flows such that the fluvial environment is changed from a fast flowing stream to one with slow, diffuse flow. These processes appear to lead to the formation of organic sediment, accumulating to form a deep peat basin. The sustained input of water from the folded and fractured quartzite lithologies of the Cape Supergroup that make up the Langeberg Mountains, which provide the bulk of the water supply to the wetland, is also important in promoting permanent flooding in the wetland. A feature that characterized the wetland was the fact that bedrock across the valley beneath the peat deposits exhibited a remarkably uniform elevation. This suggests that over long periods of time (tens to hundreds of thousands of years), bedrock has been laterally planed across the valley floor. It is proposed that valley widening associated with lateral planning of Uitenhage Formation rocks has taken place during periods of episodic very high flows. During these episodes, erosion cuts into the peat wetland and valley sides, cutting to bedrock and planing the valley floor to a uniform elevation for a given distance from the head of the wetland. Periods of episodic degradation are followed by periods of renewed peat accumulation associated with palmiet establishment, such that the wetland valley is shaped by repeated cycles of cutting and filling. Palmiet can be considered an “ecosystem engineer” that is integral to the formation of these deep peat basins. Removal of palmiet from these systems is likely to have negative consequences for the wetland and its functions in that water storage will be reduced, erosion will increase dramatically, and the water-purification function of the wetlands will be lost. Management of these wetlands, which are close to the geomorphic threshold slopes for their size, is therefore essential if they are to be preserved for the benefit of human well-being.
- Full Text:
Gold mineralisation at Masumbi Au-Cu Prospect, west Kenya : implication for gold exploration in the Archaean Ndori Greenstone Belt of Kenya
- Authors: Salimo, Luckmore
- Date: 2014
- Subjects: Gold mines and mining -- Kenya -- Nyanza Province , Copper -- Kenya -- Nyanza Province , Prospecting -- Kenya -- Nyanza Province , Chalcopyrite -- Kenya -- Nyanza Province , Metamorphism (Geology) , Geochemistry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5096 , http://hdl.handle.net/10962/d1020961
- Description: The Masumbi Au-Cu deposit in the Ndori Greenstone Belt of western Kenya is hosted in dacitic volcanics of the Nyanzian Group (2710 ± 340 Ma) and dioritic to granodioritic felsic intrusives (2504 ± 48 Ma). The deposit is characterised by gold and copper mineralisation that is associated with quartz-sulphide veins and veinlets. The copper mineralisation typically occurs as chalcopyrite. Gold is closely associated with pyrite in mineralogy and its pathfinder elements silver, bismuth, tellurium and selenium in geochemistry. The gold occurs in two forms that may indicate two generations of precipitation: the equant and the elongate forms. Based on Au/Ag ratios, the equant gold grains can be classified as native gold as their gold content is greater than 90 wt%. The elongate gold grains can be classified as electrums as their silver content is greater than 38 wt%. While there is a strong Au-Ag association within individual gold grains supporting an orogenic model for the gold mineralisation, mineralisation at the Masumbi Prospect appears atypical of Archaean orogenic gold deposits because of the abundance of copper (up to 0.43%). The enrichment of silver, copper, bismuth and tellurium in ore assemblages is common in porphyry, VMS and epithermal systems, but their presence at Masumbi does not preclude the formation as an orogenic deposit. Assay results from three Masumbi diamond drill-holes show an apparent correlation between gold and copper. However, petrography and electron probe microanalyses results from this study indicate that chalcopyrite is an earlier phase than pyrite as it occasionally occurs as inclusions in pyrite. This petrogenetic relationship between pyrite and chalcopyrite suggests that there is no temporal relationship between gold and copper mineralisation. Statistical analysis of the assays shows no linear correlation between gold and copper thereby supporting the above findings. The gold and copper mineralisation have been interpreted as forming as two separate events with copper forming first followed by gold. These events are both related to the intrusion of the felsic rocks that are associated with the Aruan metamorphic event that has been responsible for the bulk of the gold mineralisation on the Tanzanian Craton. The common alteration assemblage in the Masumbi rocks comprises chlorite and epidote. This alteration assemblage is typical of regional greenschist metamorphic facies grading into amphibolite metamorphic facies in the Nyanzian Group of Kenya. However, these alteration minerals could possibly be products of propylitic alteration in the rock groundmass. Other alteration mineral assemblages, possibly of hydrothermal origin, comprise muscovite, sericite, quartz, carbonate, associated with the sulphides pyrite and chalcopyrite. Although the occurrence of gold appears to be controlled by the presence of pyrite, it is also associated with silicification. Exploration methods have been proposed to target undiscovered gold deposits in the Ndori Greenstone Belt that are similar to the Masumbi deposit. These methods could probably be applied to vein-type gold deposits in other granite-greenstone terranes in the Lake Victoria Goldfields.
- Full Text:
- Authors: Salimo, Luckmore
- Date: 2014
- Subjects: Gold mines and mining -- Kenya -- Nyanza Province , Copper -- Kenya -- Nyanza Province , Prospecting -- Kenya -- Nyanza Province , Chalcopyrite -- Kenya -- Nyanza Province , Metamorphism (Geology) , Geochemistry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5096 , http://hdl.handle.net/10962/d1020961
- Description: The Masumbi Au-Cu deposit in the Ndori Greenstone Belt of western Kenya is hosted in dacitic volcanics of the Nyanzian Group (2710 ± 340 Ma) and dioritic to granodioritic felsic intrusives (2504 ± 48 Ma). The deposit is characterised by gold and copper mineralisation that is associated with quartz-sulphide veins and veinlets. The copper mineralisation typically occurs as chalcopyrite. Gold is closely associated with pyrite in mineralogy and its pathfinder elements silver, bismuth, tellurium and selenium in geochemistry. The gold occurs in two forms that may indicate two generations of precipitation: the equant and the elongate forms. Based on Au/Ag ratios, the equant gold grains can be classified as native gold as their gold content is greater than 90 wt%. The elongate gold grains can be classified as electrums as their silver content is greater than 38 wt%. While there is a strong Au-Ag association within individual gold grains supporting an orogenic model for the gold mineralisation, mineralisation at the Masumbi Prospect appears atypical of Archaean orogenic gold deposits because of the abundance of copper (up to 0.43%). The enrichment of silver, copper, bismuth and tellurium in ore assemblages is common in porphyry, VMS and epithermal systems, but their presence at Masumbi does not preclude the formation as an orogenic deposit. Assay results from three Masumbi diamond drill-holes show an apparent correlation between gold and copper. However, petrography and electron probe microanalyses results from this study indicate that chalcopyrite is an earlier phase than pyrite as it occasionally occurs as inclusions in pyrite. This petrogenetic relationship between pyrite and chalcopyrite suggests that there is no temporal relationship between gold and copper mineralisation. Statistical analysis of the assays shows no linear correlation between gold and copper thereby supporting the above findings. The gold and copper mineralisation have been interpreted as forming as two separate events with copper forming first followed by gold. These events are both related to the intrusion of the felsic rocks that are associated with the Aruan metamorphic event that has been responsible for the bulk of the gold mineralisation on the Tanzanian Craton. The common alteration assemblage in the Masumbi rocks comprises chlorite and epidote. This alteration assemblage is typical of regional greenschist metamorphic facies grading into amphibolite metamorphic facies in the Nyanzian Group of Kenya. However, these alteration minerals could possibly be products of propylitic alteration in the rock groundmass. Other alteration mineral assemblages, possibly of hydrothermal origin, comprise muscovite, sericite, quartz, carbonate, associated with the sulphides pyrite and chalcopyrite. Although the occurrence of gold appears to be controlled by the presence of pyrite, it is also associated with silicification. Exploration methods have been proposed to target undiscovered gold deposits in the Ndori Greenstone Belt that are similar to the Masumbi deposit. These methods could probably be applied to vein-type gold deposits in other granite-greenstone terranes in the Lake Victoria Goldfields.
- Full Text:
Gold mineralization in a high grade metamorphic terrane in the Handeni District, Eastern Tanzania
- Bitesigirwe, Godfrey Stephen
- Authors: Bitesigirwe, Godfrey Stephen
- Date: 2014
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/54756 , vital:26609
- Description: Most orogenic type gold deposits are formed under low greenschist facies to mid amphibolite facies metamorphic conditions and deposition is either structurally or lithologically controlled. A few known gold deposits found in high grade metamorphic terranes include those in the Yilgarn craton in Australia, Renco in Zimbabwe, Hemlo in Canada and the recently discovered Handeni deposit in Tanzania. Within Tanzania, gold deposits are mainly hosted in Archaean low grade metamorphic rocks commonly known as the Lake Victoria greenstone belt. The greenstone belts of Tanzania are of Nyanzian age (> 2.5Ga) and are located to the south and east of Lake Victoria on the Tanzania craton. The Tanzania Craton is surrounded by Usagaran 1.9 Ga rocks (the east African orogenic belt (EAO) better known as the Mozambique belt) to the east and the Ubendian belt to the south and west. Published reports show that the eastern part of the Tanzania Craton is dominated by the fragments of Archaean rocks. Metamorphism along East Africa and the Tanzania Craton is due to several geological events. These geological events include the intrusion of granites in the Archaean Tanzania Craton (3 Ga), subduction of ocean plate resulted to the formation of Usagaran belt (1.9 Ga), opening and closure of Mozambique Ocean, which resulted in the formation of the Mozambique belt between 700 – 800 Ma and the Pan African orogeny at 640 – 620 Ma, which is associated with the formation of Gondwana. It is believed that fragments from the Archaean Tanzania craton were re - metamorphosed during these events. The Handeni project (the focus of this thesis) is located in the northern portion of the eastern part of the Usagaran belt (1.9 Ga) comprising the eastern part of Archaean Tanzania Craton. The area is characterized by Proterozic rocks of basaltic composition. The documented 2.7 Ga rocks at the Kilindi Handeni Superterrane at the northern part of the Usagaran belt correlate well with 2.7 Ga of Nyanzian rocks of Archaean Tanzania craton. The Handeni project area is geologically dominated by metamorphosed and deformed units of quartzofeldspathic gneisses, migmatitic gneiss, garnet silicified rock, garnetiferous amphibolite, garnetiferous granulite, graphitic schist and hornblende pyroxenite. Intensive deformation features that were developed include folds (sheath folds, micro and macro scales), faults, shears and regional thrusts. This thesis focuses on identifying the protolith of the rocks, alteration minerals, and metamorphic assemblages in the project area in order to understand the timing of gold mineralization. Geological investigation of core, ore petrology and mineralogy, mineral composition by using JEOL microprobe analysis and XRF analysis of bulk rocks were utilized. All the analytical work was done at the Geology laboratory, Rhodes University. Petrographic analysis shows that the rocks sampled in the study area are characterized by alteration minerals such as calcite, dolomite and sericite. Sulphide minerals including chalcopyrite, pyrrhotite, pyrite, pentlandite and gersdorffite were identified. Gold mineralization is associated with disseminated sulphides in association with trace amounts of base metals. Four rock types were proposed as host rocks for the mineralization, namely garnet silicified rock with superimposed quartz veins, garnetiferous amphibolite, garnetiferous granulite and hornblende pyroxenite. Fold troughs, filled fractures associated with episodes of folding, quartz veins and shear zones are suggested as gold precipitation sites. The presence of high grade metamorphic rocks containing gold, intermediate to low grade assemblages with sulphides and associated hydrothermal alteration as well as a complex deformation history suggests that the Handeni mineralization took place over an extended time period stretching from a ductile to a brittle environment.
- Full Text:
- Authors: Bitesigirwe, Godfrey Stephen
- Date: 2014
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/54756 , vital:26609
- Description: Most orogenic type gold deposits are formed under low greenschist facies to mid amphibolite facies metamorphic conditions and deposition is either structurally or lithologically controlled. A few known gold deposits found in high grade metamorphic terranes include those in the Yilgarn craton in Australia, Renco in Zimbabwe, Hemlo in Canada and the recently discovered Handeni deposit in Tanzania. Within Tanzania, gold deposits are mainly hosted in Archaean low grade metamorphic rocks commonly known as the Lake Victoria greenstone belt. The greenstone belts of Tanzania are of Nyanzian age (> 2.5Ga) and are located to the south and east of Lake Victoria on the Tanzania craton. The Tanzania Craton is surrounded by Usagaran 1.9 Ga rocks (the east African orogenic belt (EAO) better known as the Mozambique belt) to the east and the Ubendian belt to the south and west. Published reports show that the eastern part of the Tanzania Craton is dominated by the fragments of Archaean rocks. Metamorphism along East Africa and the Tanzania Craton is due to several geological events. These geological events include the intrusion of granites in the Archaean Tanzania Craton (3 Ga), subduction of ocean plate resulted to the formation of Usagaran belt (1.9 Ga), opening and closure of Mozambique Ocean, which resulted in the formation of the Mozambique belt between 700 – 800 Ma and the Pan African orogeny at 640 – 620 Ma, which is associated with the formation of Gondwana. It is believed that fragments from the Archaean Tanzania craton were re - metamorphosed during these events. The Handeni project (the focus of this thesis) is located in the northern portion of the eastern part of the Usagaran belt (1.9 Ga) comprising the eastern part of Archaean Tanzania Craton. The area is characterized by Proterozic rocks of basaltic composition. The documented 2.7 Ga rocks at the Kilindi Handeni Superterrane at the northern part of the Usagaran belt correlate well with 2.7 Ga of Nyanzian rocks of Archaean Tanzania craton. The Handeni project area is geologically dominated by metamorphosed and deformed units of quartzofeldspathic gneisses, migmatitic gneiss, garnet silicified rock, garnetiferous amphibolite, garnetiferous granulite, graphitic schist and hornblende pyroxenite. Intensive deformation features that were developed include folds (sheath folds, micro and macro scales), faults, shears and regional thrusts. This thesis focuses on identifying the protolith of the rocks, alteration minerals, and metamorphic assemblages in the project area in order to understand the timing of gold mineralization. Geological investigation of core, ore petrology and mineralogy, mineral composition by using JEOL microprobe analysis and XRF analysis of bulk rocks were utilized. All the analytical work was done at the Geology laboratory, Rhodes University. Petrographic analysis shows that the rocks sampled in the study area are characterized by alteration minerals such as calcite, dolomite and sericite. Sulphide minerals including chalcopyrite, pyrrhotite, pyrite, pentlandite and gersdorffite were identified. Gold mineralization is associated with disseminated sulphides in association with trace amounts of base metals. Four rock types were proposed as host rocks for the mineralization, namely garnet silicified rock with superimposed quartz veins, garnetiferous amphibolite, garnetiferous granulite and hornblende pyroxenite. Fold troughs, filled fractures associated with episodes of folding, quartz veins and shear zones are suggested as gold precipitation sites. The presence of high grade metamorphic rocks containing gold, intermediate to low grade assemblages with sulphides and associated hydrothermal alteration as well as a complex deformation history suggests that the Handeni mineralization took place over an extended time period stretching from a ductile to a brittle environment.
- Full Text:
Host relations of Kalaharituber pfeilii (Henn.) Trappe & Kagan-Zur
- Authors: Ntshakaza, Pamella
- Date: 2014
- Subjects: Ascomycetes -- Kalahari Desert , Medicinal plants -- Kalahari Desert , Edible fungi -- Kalahari Desert , Truffles -- Kalahari Desert , Desert plants -- Kalahari Desert , Mycorrhizas -- Kalahari Desert , Stipa -- Kalahari Desert
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4167 , http://hdl.handle.net/10962/d1020888
- Description: Kalaharituber pfeilii (Henn.) Trappe & Kagan-Zur commonly known as the “Kalahari truffle” is a desert truffle species identified from the Kalahari region of southern Africa. Two other species, Eremiomyces echinulatus (Trappe & Marasas) Trappe & Kagan-Zur and Mattirolomyces austroafricanus (Trappe & Marasas) Trappe & Kovacs are also known to occur in other parts of southern Africa. Truffles are hypogeous fruiting bodies of Ascomycetes, important to humans for their nutritional value and medicinal characteristics. These truffles are known as desert truffles as they prefer to occur under arid or semi-arid conditions characteristic of deserts. Truffle development depends on the presence of a mycorrhizal host, associated microorganisms as well as soil and climatic characteristics. It has been suggested that K. pfeilii has a suspected broad plant host range which includes herbaceous to woody trees and shrubs. However, these relationships have not been verified. Indigenous people of the Kalahari believe that truffles are found under grasses. In the Kalahari, truffle fruiting bodies are often found entangled in Stipagrostis ciliata (Desf.) De Winter var. capensis (Trin. & Rupr.) De Winter roots. S. ciliata, also known as the tall bushman-grass, is the most common grass found in the Kalahari. The objective of this study was to provide conclusive evidence that S. ciliata var. capensis is a host of the Kalahari truffle. Truffle fruiting bodies and grass roots from where the truffles were found were collected from Upington, South Africa. The fruiting bodies were identified by observing their morphological characteristics using the ‘Keys of Truffle genera’. All observed physical properties were similar to those of K. pfeilii and further identification was done using molecular techniques. DNA was extracted from the fruiting bodies, mycelial cultures, rhizosheaths and from the S. ciliata var. capensis grass roots, which were then amplified using the specific K. pfeilii specific primers TPF3 and TPR1 and sequenced. The obtained sequence results confirmed that the collected fruiting bodies were those of the K. pfeilii and the molecular techniques also confirmed that K. pfeilii DNA was present in the S. ciliata var. capensis rhizosheath and root cells. Microscopy showed an ectendomycorrhizal association between K. pfeilii and S. ciliata var. capensis. Mycorrhizal resynthesis experiments were conducted to establish this mycorrhizal association in-vitro. They were unsuccessful because of the structure of the grass and the availability of contaminants. And more...
- Full Text:
- Authors: Ntshakaza, Pamella
- Date: 2014
- Subjects: Ascomycetes -- Kalahari Desert , Medicinal plants -- Kalahari Desert , Edible fungi -- Kalahari Desert , Truffles -- Kalahari Desert , Desert plants -- Kalahari Desert , Mycorrhizas -- Kalahari Desert , Stipa -- Kalahari Desert
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4167 , http://hdl.handle.net/10962/d1020888
- Description: Kalaharituber pfeilii (Henn.) Trappe & Kagan-Zur commonly known as the “Kalahari truffle” is a desert truffle species identified from the Kalahari region of southern Africa. Two other species, Eremiomyces echinulatus (Trappe & Marasas) Trappe & Kagan-Zur and Mattirolomyces austroafricanus (Trappe & Marasas) Trappe & Kovacs are also known to occur in other parts of southern Africa. Truffles are hypogeous fruiting bodies of Ascomycetes, important to humans for their nutritional value and medicinal characteristics. These truffles are known as desert truffles as they prefer to occur under arid or semi-arid conditions characteristic of deserts. Truffle development depends on the presence of a mycorrhizal host, associated microorganisms as well as soil and climatic characteristics. It has been suggested that K. pfeilii has a suspected broad plant host range which includes herbaceous to woody trees and shrubs. However, these relationships have not been verified. Indigenous people of the Kalahari believe that truffles are found under grasses. In the Kalahari, truffle fruiting bodies are often found entangled in Stipagrostis ciliata (Desf.) De Winter var. capensis (Trin. & Rupr.) De Winter roots. S. ciliata, also known as the tall bushman-grass, is the most common grass found in the Kalahari. The objective of this study was to provide conclusive evidence that S. ciliata var. capensis is a host of the Kalahari truffle. Truffle fruiting bodies and grass roots from where the truffles were found were collected from Upington, South Africa. The fruiting bodies were identified by observing their morphological characteristics using the ‘Keys of Truffle genera’. All observed physical properties were similar to those of K. pfeilii and further identification was done using molecular techniques. DNA was extracted from the fruiting bodies, mycelial cultures, rhizosheaths and from the S. ciliata var. capensis grass roots, which were then amplified using the specific K. pfeilii specific primers TPF3 and TPR1 and sequenced. The obtained sequence results confirmed that the collected fruiting bodies were those of the K. pfeilii and the molecular techniques also confirmed that K. pfeilii DNA was present in the S. ciliata var. capensis rhizosheath and root cells. Microscopy showed an ectendomycorrhizal association between K. pfeilii and S. ciliata var. capensis. Mycorrhizal resynthesis experiments were conducted to establish this mycorrhizal association in-vitro. They were unsuccessful because of the structure of the grass and the availability of contaminants. And more...
- Full Text:
Imagine/nation : mediating 'xenophobia' through visual and performance art
- Machona, Gerald Ralph Tawanda
- Authors: Machona, Gerald Ralph Tawanda
- Date: 2014
- Subjects: Xenophobia -- South Africa , Xenophobia in mass media , Performance art , Immigrants in art , Violence in art , Race in art
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:2480 , http://hdl.handle.net/10962/d1011106 , Xenophobia -- South Africa , Xenophobia in mass media , Performance art , Immigrants in art , Violence in art , Race in art
- Description: This half-thesis has developed as a supporting document to an exhibition titled Vabvakure, people from far away, which responds to the growing trends of violence perpetrated against African foreign nationals living in South Africa. This violence which has generally been termed as 'xenophobia' has been framed within this discourse as 'afrophobia', as it is fraught with complexities of race, ethnicity and class. Evidently, not all foreign nationals are at risk but selective targeting of working class black African foreign nationals seems to be the modus operandi. Fanning these flames of prejudice are stereotypes and negative perceptions of Africa and African immigrants that have permeated into the national consciousness of South Africa, which the mainstream media has been complicit in cultivating. My practice is concerned with challenging this politic of representation in relation to the image of the African foreign national within South African society, who have been presented negatively and labelled as the 'Makwerekwere', the 'bogeymen' that have been blamed for the country’s current woes. In response to this, my research adopts the premise that forms of cultural mediation such as visual and performance art can offer further insights and possibly yield solutions that can be used to address these sentiments. As globalisation and neoliberal ideologies reshape the world, there is a growing need in the post-colonial state to revisit and re-construct notions of individual and collective identity, especially that of the nation. Nations, nationalisms and citizenry can no longer be defined solely through indigeneity, for as a result of radical shifts in the flow of migration and immigration policies that allow for naturalisation of aliens and foreign nationals, we are now faced with burgeoning levels of social diversity to the extent that constructions of nationhood that are based on the concept of autochthony have resulted in the persecution of the ‘other’.
- Full Text:
- Authors: Machona, Gerald Ralph Tawanda
- Date: 2014
- Subjects: Xenophobia -- South Africa , Xenophobia in mass media , Performance art , Immigrants in art , Violence in art , Race in art
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:2480 , http://hdl.handle.net/10962/d1011106 , Xenophobia -- South Africa , Xenophobia in mass media , Performance art , Immigrants in art , Violence in art , Race in art
- Description: This half-thesis has developed as a supporting document to an exhibition titled Vabvakure, people from far away, which responds to the growing trends of violence perpetrated against African foreign nationals living in South Africa. This violence which has generally been termed as 'xenophobia' has been framed within this discourse as 'afrophobia', as it is fraught with complexities of race, ethnicity and class. Evidently, not all foreign nationals are at risk but selective targeting of working class black African foreign nationals seems to be the modus operandi. Fanning these flames of prejudice are stereotypes and negative perceptions of Africa and African immigrants that have permeated into the national consciousness of South Africa, which the mainstream media has been complicit in cultivating. My practice is concerned with challenging this politic of representation in relation to the image of the African foreign national within South African society, who have been presented negatively and labelled as the 'Makwerekwere', the 'bogeymen' that have been blamed for the country’s current woes. In response to this, my research adopts the premise that forms of cultural mediation such as visual and performance art can offer further insights and possibly yield solutions that can be used to address these sentiments. As globalisation and neoliberal ideologies reshape the world, there is a growing need in the post-colonial state to revisit and re-construct notions of individual and collective identity, especially that of the nation. Nations, nationalisms and citizenry can no longer be defined solely through indigeneity, for as a result of radical shifts in the flow of migration and immigration policies that allow for naturalisation of aliens and foreign nationals, we are now faced with burgeoning levels of social diversity to the extent that constructions of nationhood that are based on the concept of autochthony have resulted in the persecution of the ‘other’.
- Full Text:
Impact of batting skill on pacing during repeated sprints between the wickets.
- Authors: Elliott, Andrea
- Date: 2014
- Subjects: Cricket -- Batting -- Research , Sprinting -- Research , Cricket players , Exercise -- Physiological aspects -- Research , Sports -- Physiological aspects -- Research
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5153 , http://hdl.handle.net/10962/d1013546
- Description: Introduction: With batting in cricket, there is no known end point, making the allocation of resources and the development of a suitable pacing strategy more difficult. How batsmen allocate resources and pace themselves when repeatedly sprinting between the wickets is therefore not known. According to the ‘anticipatory feedback’ model, the level of expertise/experience has a substantial influence on the development of a suitable pacing strategy. Skilled or experienced batsmen may therefore have a greater ability to develop and implement a pacing strategy compared to that of novice or less-skilled batsmen. Purpose: To assess whether the absence of a known end point at the beginning of repeated sprint bouts between the wickets effects how batsmen pace themselves comparing skilled and less-skilled batsmen. Methods: Twenty-four male cricketers from a university league were selected. Twelve skilled batsmen (players in the top five batting order), and 12 less-skilled batsmen (players in the bottom five batting order) completed three experimental. All trials required batsmen to complete the same number of shuttle sprints (14 shuttles and therefore 28 runs), while only the information provided before each trial differed. Control Trial: This trial is also referred to as the ‘informed’ trial as the batsmen are aware of the exercise requirements (end point). Unknown Trial: The batsmen were not informed of the exercise end point and were required to run on command for an indefinite period (28 runs). Deceptive Trial: Batsmen’ were incorrectly informed with regards to the number of sprints (told they were only doing 14 runs when in fact they were doing 28 runs). Muscle activity, sprint times and RPE measures were obtained during all three trials and compared. Results: Significant (p<0.05) group effects were apparent for sprint times and, ‘central’ and ‘local’ RPE. Specifically, skilled batsmen obtained the faster mean sprint times, and less-skilled batsmen reported the higher mean ‘central’ and ‘local’ RPE values in all three experimental trials. There were no significant group effects for muscle activation. Skilled batsmen did however have lower muscle activation compared to less-skilled batsmen in all three trials. Furthermore, general trends revealed that sprint times and muscle activation decreased over time (from shuttle 1 to shuttle 14), and RPE (‘central’ and ‘local’) ratings increased, regardless of the experimental trials. Skilled batsmen performed the best in all three trials. This was determined by the attainment of faster sprint times, lower muscle activation and low RPE ratings in each of the three trials. The assessment of the relationship of the dependent variables measured in each trial, did however suggest that skilled batsmen performed best in the deceptive trial while less-skilled batsmen performed best in the control trial. Skilled batsmen thus showed superior performance when trial requirements were unclear. Less-skilled batsmen however, were seen to experience decrements in performance when information regarding the end point was unknown or misleading. Conclusion: It can thus be concluded that previous experience facilitates in the obtainment of improved sprint times between the wickets, especially when the exact end point is unknown.
- Full Text:
- Authors: Elliott, Andrea
- Date: 2014
- Subjects: Cricket -- Batting -- Research , Sprinting -- Research , Cricket players , Exercise -- Physiological aspects -- Research , Sports -- Physiological aspects -- Research
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5153 , http://hdl.handle.net/10962/d1013546
- Description: Introduction: With batting in cricket, there is no known end point, making the allocation of resources and the development of a suitable pacing strategy more difficult. How batsmen allocate resources and pace themselves when repeatedly sprinting between the wickets is therefore not known. According to the ‘anticipatory feedback’ model, the level of expertise/experience has a substantial influence on the development of a suitable pacing strategy. Skilled or experienced batsmen may therefore have a greater ability to develop and implement a pacing strategy compared to that of novice or less-skilled batsmen. Purpose: To assess whether the absence of a known end point at the beginning of repeated sprint bouts between the wickets effects how batsmen pace themselves comparing skilled and less-skilled batsmen. Methods: Twenty-four male cricketers from a university league were selected. Twelve skilled batsmen (players in the top five batting order), and 12 less-skilled batsmen (players in the bottom five batting order) completed three experimental. All trials required batsmen to complete the same number of shuttle sprints (14 shuttles and therefore 28 runs), while only the information provided before each trial differed. Control Trial: This trial is also referred to as the ‘informed’ trial as the batsmen are aware of the exercise requirements (end point). Unknown Trial: The batsmen were not informed of the exercise end point and were required to run on command for an indefinite period (28 runs). Deceptive Trial: Batsmen’ were incorrectly informed with regards to the number of sprints (told they were only doing 14 runs when in fact they were doing 28 runs). Muscle activity, sprint times and RPE measures were obtained during all three trials and compared. Results: Significant (p<0.05) group effects were apparent for sprint times and, ‘central’ and ‘local’ RPE. Specifically, skilled batsmen obtained the faster mean sprint times, and less-skilled batsmen reported the higher mean ‘central’ and ‘local’ RPE values in all three experimental trials. There were no significant group effects for muscle activation. Skilled batsmen did however have lower muscle activation compared to less-skilled batsmen in all three trials. Furthermore, general trends revealed that sprint times and muscle activation decreased over time (from shuttle 1 to shuttle 14), and RPE (‘central’ and ‘local’) ratings increased, regardless of the experimental trials. Skilled batsmen performed the best in all three trials. This was determined by the attainment of faster sprint times, lower muscle activation and low RPE ratings in each of the three trials. The assessment of the relationship of the dependent variables measured in each trial, did however suggest that skilled batsmen performed best in the deceptive trial while less-skilled batsmen performed best in the control trial. Skilled batsmen thus showed superior performance when trial requirements were unclear. Less-skilled batsmen however, were seen to experience decrements in performance when information regarding the end point was unknown or misleading. Conclusion: It can thus be concluded that previous experience facilitates in the obtainment of improved sprint times between the wickets, especially when the exact end point is unknown.
- Full Text:
Influence of non-synonymous sequence mutations on the architecture of HIV-1 clade C protease receptor site : docking and molecular dynamics studies
- Authors: Onywera, David Harris
- Date: 2014
- Subjects: HIV (Viruses) -- Research , HIV infections -- Treatment -- Research , HIV infections -- Chemotherapy , Protease inhibitors -- Research , Viruses -- Effect of drugs on -- Research , Antiretroviral agents
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4116 , http://hdl.handle.net/10962/d1013133
- Description: Despite the current interventions to avert contagions and AIDS-related deaths, sub-Saharan Africa is still the region most severely affected by the HIV/AIDS pandemic, where clade C is the dominant circulating HIV-1 strain. The pol-encoded HIV-1 protease enzyme has been extensively exploited as a drug target. Protease inhibitors have been engineered within the framework of clade B, the commonest in America, Europe and Australia. Recent studies have attested the existence of sequence and catalytic disparities between clades B and C proteases that could upset drug susceptibilities. Emergence of drug-resistant associated mutations and combinatorial explosions due to recombination thwarts the attempt to stabilize the current highly active antiretroviral therapy (HAART) baseline. The project aimed at identifying the structural and molecular mechanisms hired by mutants to affect the efficacies of both FDA approved and Rhodes University (RU)-synthesized inhibitors, in order to define how current and or future drugs ought to be modified or synthesized with the intent of combating drug resistance. The rationale involved the generation of homology models of the HIV-1 sequences from the South African infants failing treatment with two protease inhibitors: lopinavir and ritonavir (as monitored by alterations in surrogate markers: CD4 cell count decline and viral load upsurge). Consistent with previous studies, we established nine polymorphisms: 12S, 15V, 19I, 36I, 41K, 63P, 69K, 89M, and 93L, linked to subtype C wild-type; some of which are associated with protease treatment in clade B. Even though we predicted two occurrence patterns of M46I, I54V and V82A mutations as V82A→I54V→M46I and I54V→V82A→M46V, other possibilities might exist. Mutations either caused a protracted or contracted active site cleft, which enforced differential drug responses. The in silico docking indicated susceptibility discordances between clades B and C in certain polymorphisms and non-polymorphisms. The RU-synthesized ligands displayed varied efficacies that were below those of the FDA approved protease inhibitors. The flaps underwent a wide range of structural motions to accommodate and stabilize the ligands. Computational analyses unravelled the need for these potential drugs to be restructured by (de novo) drug engineers to improve their binding fits, affinities, energies and interactions with multiple key protease residues in order to target resilient HIV-1 assemblages. Accumulating evidences on contrasting drug-choice interpretations from the Stanford HIVdb should act as an impetus for the customization of a HIVdb for the sub-Saharan subcontinent.
- Full Text:
- Authors: Onywera, David Harris
- Date: 2014
- Subjects: HIV (Viruses) -- Research , HIV infections -- Treatment -- Research , HIV infections -- Chemotherapy , Protease inhibitors -- Research , Viruses -- Effect of drugs on -- Research , Antiretroviral agents
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4116 , http://hdl.handle.net/10962/d1013133
- Description: Despite the current interventions to avert contagions and AIDS-related deaths, sub-Saharan Africa is still the region most severely affected by the HIV/AIDS pandemic, where clade C is the dominant circulating HIV-1 strain. The pol-encoded HIV-1 protease enzyme has been extensively exploited as a drug target. Protease inhibitors have been engineered within the framework of clade B, the commonest in America, Europe and Australia. Recent studies have attested the existence of sequence and catalytic disparities between clades B and C proteases that could upset drug susceptibilities. Emergence of drug-resistant associated mutations and combinatorial explosions due to recombination thwarts the attempt to stabilize the current highly active antiretroviral therapy (HAART) baseline. The project aimed at identifying the structural and molecular mechanisms hired by mutants to affect the efficacies of both FDA approved and Rhodes University (RU)-synthesized inhibitors, in order to define how current and or future drugs ought to be modified or synthesized with the intent of combating drug resistance. The rationale involved the generation of homology models of the HIV-1 sequences from the South African infants failing treatment with two protease inhibitors: lopinavir and ritonavir (as monitored by alterations in surrogate markers: CD4 cell count decline and viral load upsurge). Consistent with previous studies, we established nine polymorphisms: 12S, 15V, 19I, 36I, 41K, 63P, 69K, 89M, and 93L, linked to subtype C wild-type; some of which are associated with protease treatment in clade B. Even though we predicted two occurrence patterns of M46I, I54V and V82A mutations as V82A→I54V→M46I and I54V→V82A→M46V, other possibilities might exist. Mutations either caused a protracted or contracted active site cleft, which enforced differential drug responses. The in silico docking indicated susceptibility discordances between clades B and C in certain polymorphisms and non-polymorphisms. The RU-synthesized ligands displayed varied efficacies that were below those of the FDA approved protease inhibitors. The flaps underwent a wide range of structural motions to accommodate and stabilize the ligands. Computational analyses unravelled the need for these potential drugs to be restructured by (de novo) drug engineers to improve their binding fits, affinities, energies and interactions with multiple key protease residues in order to target resilient HIV-1 assemblages. Accumulating evidences on contrasting drug-choice interpretations from the Stanford HIVdb should act as an impetus for the customization of a HIVdb for the sub-Saharan subcontinent.
- Full Text:
Invariant optimal control on the three-dimensional semi-Euclidean group: control affine and quadratic Hamilton-Poisson systems
- Authors: Barrett, Dennis Ian
- Date: 2014
- Subjects: Automorphisms , Symmetry (Mathematics) , Lyapunov stability , Geometry, Riemannian , Geometry, Affine , Elliptic functions
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/64805 , vital:28605
- Description: In this thesis we consider invariant control systems and Hamilton-Poisson systems on the three dimensional semi-Euclidean group SE(1,1). We first classify the left-invariant control affine systems (under detached feedback equivalence). We provide a complete list of normal forms, as well as classifying conditions. As a corollary to this classification, we derive controllability criteria for control affine systems on SE(1,1). Secondly, we consider quadratic Hamilton-Poisson systems on the (minus) Lie-Poisson space se(1,1)*. These systems are classified up to an affine isomorphism. Six normal forms are identified for the homogeneous case, whereas sixteen representatives (including several infinite families) are obtained for the inhomogeneous systems. Thereafter we consider the stability and integration of the normal forms obtained. For all homogeneous systems, and a subclass of inhomogeneous systems, we perform a complete stability analysis and derive explicit expressions for all integral curves. (The extremal controls of a large class of optimal control problems on SE(1,1) are linearly related to these integral curves.) Lastly, we discuss the Riemannian and sub-Riemannian problems. The (left-invariant) Riemannian and sub-Riemannian structures on SE(1,1) are classified, up to isometric group automorphisms and scaling. Explicit expressions for the geodesics of the normalised structures are found.
- Full Text:
- Authors: Barrett, Dennis Ian
- Date: 2014
- Subjects: Automorphisms , Symmetry (Mathematics) , Lyapunov stability , Geometry, Riemannian , Geometry, Affine , Elliptic functions
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/64805 , vital:28605
- Description: In this thesis we consider invariant control systems and Hamilton-Poisson systems on the three dimensional semi-Euclidean group SE(1,1). We first classify the left-invariant control affine systems (under detached feedback equivalence). We provide a complete list of normal forms, as well as classifying conditions. As a corollary to this classification, we derive controllability criteria for control affine systems on SE(1,1). Secondly, we consider quadratic Hamilton-Poisson systems on the (minus) Lie-Poisson space se(1,1)*. These systems are classified up to an affine isomorphism. Six normal forms are identified for the homogeneous case, whereas sixteen representatives (including several infinite families) are obtained for the inhomogeneous systems. Thereafter we consider the stability and integration of the normal forms obtained. For all homogeneous systems, and a subclass of inhomogeneous systems, we perform a complete stability analysis and derive explicit expressions for all integral curves. (The extremal controls of a large class of optimal control problems on SE(1,1) are linearly related to these integral curves.) Lastly, we discuss the Riemannian and sub-Riemannian problems. The (left-invariant) Riemannian and sub-Riemannian structures on SE(1,1) are classified, up to isometric group automorphisms and scaling. Explicit expressions for the geodesics of the normalised structures are found.
- Full Text:
Investigating the role of mycorrhizal fungi and associated bacteria in promoting growth of citrus seedlings
- Authors: Sitole, Phumeza
- Date: 2014
- Subjects: Mycorrhizal fungi , Citrus -- South Africa , Citrus -- Diseases and pests -- Biological control -- South Africa , Fungi as biological pest control agents , Bacteria , Phytophthora , Pythium , Indoleacetic acid
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4111 , http://hdl.handle.net/10962/d1013033
- Description: South Africa is the world's second largest exporter of fresh citrus and is ranked 14th in citrus production. Fungal pathogens such as Phytophthora and Pythium cause economic losses as a result of root rot and brown rot. Mycorrhizal fungi are specialized members of the fungal community forming a mutualistic relationship with plant roots. Mycorrhizal fungal structures are known to associate with other soil microorganisms and these may contribute to improved plant growth. A diverse group of bacteria that interact with the mycorrhizal fungi are known as Mycorrhizal Helper Bacteria (MHB). The aim of this study was to investigate the role of arbuscular mycorrhiza and associated bacteria isolated from spores and determine whether they had any plant growth promoting potential. A total of 19 bacteria were isolated from arbuscular mycorrhizal spores and were molecularly identified as belonging to several Bacillus, Micrococcus, Onchrobactrum and Staphylococcus sp. All bacterial isolates were tested for plant growth promotion abilities. One Bacillus isolate was able to solubilise phosphate. Four isolates Micrococcus sp, Micrococcus leteus, Ochrobacterum sp and Ochrobacterum antropi were able to produce Indole Acetic Acid and three isolates showed potential to reduce growth of Phytophthora nicotianae, P. citrocola and P. citrophthora in in vitro plate cultures. Further tests using culture supernatants of the Bacillus sp, Micrococcus sp and Bacillus cereus confirmed their ability to inhibit or reduce growth of the three Phytophthora species in a 96 well bioassay. Bacillus sp and Bacillus cereus were able to inhibit Phytophthora spp by 95 to 100 % and Micrococcus spp was able to decrease pathogen growth by 60 to 94 %. These bacterial isolates were further evaluated for plant growth promoting abilities on citrus rough lemon seedlings alone or in combination with arbuscular mycorrhizal inoculum. Bacterial and mycorrhizal inoculants influence the increase in shoot and root biomass. Bacillus cereus in combination with mycorrhizal inoculum significantly increased seedling shoot to root ratio while root biomass was significantly increased with mycorrhizal inoculation. Due to the short duration of the trial mycorrhizal colonisation could not be assessed. It is evident that selected combinations of bacteria and mycorrhizal fungi could promote citrus seedling growth and potentially improve seedling health. Further studies under nursery conditions are recommended.
- Full Text:
- Authors: Sitole, Phumeza
- Date: 2014
- Subjects: Mycorrhizal fungi , Citrus -- South Africa , Citrus -- Diseases and pests -- Biological control -- South Africa , Fungi as biological pest control agents , Bacteria , Phytophthora , Pythium , Indoleacetic acid
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4111 , http://hdl.handle.net/10962/d1013033
- Description: South Africa is the world's second largest exporter of fresh citrus and is ranked 14th in citrus production. Fungal pathogens such as Phytophthora and Pythium cause economic losses as a result of root rot and brown rot. Mycorrhizal fungi are specialized members of the fungal community forming a mutualistic relationship with plant roots. Mycorrhizal fungal structures are known to associate with other soil microorganisms and these may contribute to improved plant growth. A diverse group of bacteria that interact with the mycorrhizal fungi are known as Mycorrhizal Helper Bacteria (MHB). The aim of this study was to investigate the role of arbuscular mycorrhiza and associated bacteria isolated from spores and determine whether they had any plant growth promoting potential. A total of 19 bacteria were isolated from arbuscular mycorrhizal spores and were molecularly identified as belonging to several Bacillus, Micrococcus, Onchrobactrum and Staphylococcus sp. All bacterial isolates were tested for plant growth promotion abilities. One Bacillus isolate was able to solubilise phosphate. Four isolates Micrococcus sp, Micrococcus leteus, Ochrobacterum sp and Ochrobacterum antropi were able to produce Indole Acetic Acid and three isolates showed potential to reduce growth of Phytophthora nicotianae, P. citrocola and P. citrophthora in in vitro plate cultures. Further tests using culture supernatants of the Bacillus sp, Micrococcus sp and Bacillus cereus confirmed their ability to inhibit or reduce growth of the three Phytophthora species in a 96 well bioassay. Bacillus sp and Bacillus cereus were able to inhibit Phytophthora spp by 95 to 100 % and Micrococcus spp was able to decrease pathogen growth by 60 to 94 %. These bacterial isolates were further evaluated for plant growth promoting abilities on citrus rough lemon seedlings alone or in combination with arbuscular mycorrhizal inoculum. Bacterial and mycorrhizal inoculants influence the increase in shoot and root biomass. Bacillus cereus in combination with mycorrhizal inoculum significantly increased seedling shoot to root ratio while root biomass was significantly increased with mycorrhizal inoculation. Due to the short duration of the trial mycorrhizal colonisation could not be assessed. It is evident that selected combinations of bacteria and mycorrhizal fungi could promote citrus seedling growth and potentially improve seedling health. Further studies under nursery conditions are recommended.
- Full Text:
Lignocellulosic waste degradation using enzyme synergy with commercially available enzymes and Clostridium cellulovorans XylanaseA and MannanaseA
- Authors: Morrison, David Graham
- Date: 2014
- Subjects: Lignocellulose -- Biodegradation , Enzymes -- Biotechnology , Agricultural wastes as fuel , Polysaccharides -- Biotechnology , Sugar -- Inversion , Clostridium , Xylanases , Monomers
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4119 , http://hdl.handle.net/10962/d1013292
- Description: The launch of national and international initiatives to reduce pollution, reliance on fossil fuels and increase the beneficiation of agricultural wastes has prompted research into sugar monomer production from lignocellulosic wastes. These sugars can subsequently be used in the production of biofuels and environmentally degradable plastics. This study investigated the use of synergistic combinations of commercial and pure enzymes to lower enzyme costs and loadings, while increasing enzyme activity in the hydrolysis of agricultural waste. Pineapple pomace was selected due to its current underutilisation and the substantial quantities of it produced annually, as a by-product of pineapple canning. One of the primary costs in beneficiating agricultural wastes, such as pineapple pomace, is the high cost of enzyme solutions used to generate reducing sugars. This can be lowered through the use of synergistic combinations of enzymes. Studies related to the inclusion of hemicellulose degrading enzymes with commercial enzyme solutions have been limited and investigation of these solutions in select combinations, together with pineapple pomace substrate, allows for novel research. The use of synergistic combinations of purified cellulosomal enzymes has previously been shown to be effective at releasing reducing sugars from agricultural wastes. For the present study, MannanaseA and XylanaseA from Clostridium cellulovorans were heterologously expressed in Escherichia coli BL21 (DE3) cells and purified with immobilised metal affinity chromatography. These enzymes, in addition to two commercially available enzyme solutions (Celluclast 1.5L® and Pectinex® 3XL), were assayed on defined polysaccharides that are present in pineapple pomace to determine their substrate specificities. The degree(s) of synergy and specific activities of selected combinations of these enzymes were tested under both simultaneous and sequential conditions. It was observed that several synergistic combinations of enzyme solutions in select ratios, such as C20P60X20 (20% cellulose, 60% pectinase and 20% xylanse), C20P40X40 (20% cellulose, 40% pectinase and 40% xylanase) and C20P80 (20% cellulose, 80% pectinase) with pineapple pomace could both decrease the protein loading, while raising the level of activity compared to individual enzyme solutions. The highest quantity of reducing sugars to protein weight used on pineapple pomace was recorded at 3, 9 and 18 hours with combinations of Pectinex® 3XL and Celluclast 1.5L®, but for 27 h it was combinations of both these commercial solutions with XynA. The contribution of XynA was significant as C20P60X20 displayed the second highest reducing sugar production of 1.521 mg/mL, at 36 h from 12.875 μg/mL of protein, which was the second lowest protein loading. It was also shown that certain enzyme combinations, such as Pectinex® 3XL, Celluclast 1.5L® and XynA, did not generate synergy when combined in solution at the initial stages of hydrolysis, and instead generated a form of competition called anti-synergy. This was due to Pectinex® 3XL which had anti-synergy relationships in select combinations with the other enzyme solutions assayed. It was also observed that the degree of synergy and specific activity for a combination changed over time. Some solutions displayed the highest levels of synergy at the commencement of hydrolysis, namely Celluclast 1.5L®, ManA and XynA. Other combinations exhibited the highest levels of synergy at the end of the assay period, such as Pectinex® 3XL and Celluclast 1.5L®. Whether greater synergy was generated at the start or end of hydrolysis was a function of the stability of the enzymes in solution and whether enzyme activity increased substrate accessibility or generated competition between enzymes in solution. Sequential synergy studies demonstrated an anti-synergy relationship between Pectinex® 3XL and XynA or ManA, as well as Pectinex® 3 XL and Celluclast 1.5L®. It was found that under sequential synergy conditions with Pectinex® 3 XL, XynA and ManA, that anti-synergy could be negated and high degrees of synergy attained when the enzymes were added in specific loading orders and not inhibited by the presence of other active enzymes. The importance of loading order was demonstrated under sequential synergy conditions when XynA was added before ManA followed by Pectinex® 3 XL, which increased the activity and synergy of the solution by 50%. This equates to a 60% increase in reducing sugar release from the same concentrations of enzymes and emphasises the importance of removing anti-synergy relationships from combinations of enzymes. It can be concluded that a C20P60X20 combination (based on activity) can both synergistically increase the reducing sugar production and lower the protein loading required for pineapple pomace hydrolysis. This study also highlights the importance of reducing anti-synergy in customised enzyme cocktails and how sequential synergy can demonstrate the order in which a lignocellulosic waste is degraded.
- Full Text:
- Authors: Morrison, David Graham
- Date: 2014
- Subjects: Lignocellulose -- Biodegradation , Enzymes -- Biotechnology , Agricultural wastes as fuel , Polysaccharides -- Biotechnology , Sugar -- Inversion , Clostridium , Xylanases , Monomers
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4119 , http://hdl.handle.net/10962/d1013292
- Description: The launch of national and international initiatives to reduce pollution, reliance on fossil fuels and increase the beneficiation of agricultural wastes has prompted research into sugar monomer production from lignocellulosic wastes. These sugars can subsequently be used in the production of biofuels and environmentally degradable plastics. This study investigated the use of synergistic combinations of commercial and pure enzymes to lower enzyme costs and loadings, while increasing enzyme activity in the hydrolysis of agricultural waste. Pineapple pomace was selected due to its current underutilisation and the substantial quantities of it produced annually, as a by-product of pineapple canning. One of the primary costs in beneficiating agricultural wastes, such as pineapple pomace, is the high cost of enzyme solutions used to generate reducing sugars. This can be lowered through the use of synergistic combinations of enzymes. Studies related to the inclusion of hemicellulose degrading enzymes with commercial enzyme solutions have been limited and investigation of these solutions in select combinations, together with pineapple pomace substrate, allows for novel research. The use of synergistic combinations of purified cellulosomal enzymes has previously been shown to be effective at releasing reducing sugars from agricultural wastes. For the present study, MannanaseA and XylanaseA from Clostridium cellulovorans were heterologously expressed in Escherichia coli BL21 (DE3) cells and purified with immobilised metal affinity chromatography. These enzymes, in addition to two commercially available enzyme solutions (Celluclast 1.5L® and Pectinex® 3XL), were assayed on defined polysaccharides that are present in pineapple pomace to determine their substrate specificities. The degree(s) of synergy and specific activities of selected combinations of these enzymes were tested under both simultaneous and sequential conditions. It was observed that several synergistic combinations of enzyme solutions in select ratios, such as C20P60X20 (20% cellulose, 60% pectinase and 20% xylanse), C20P40X40 (20% cellulose, 40% pectinase and 40% xylanase) and C20P80 (20% cellulose, 80% pectinase) with pineapple pomace could both decrease the protein loading, while raising the level of activity compared to individual enzyme solutions. The highest quantity of reducing sugars to protein weight used on pineapple pomace was recorded at 3, 9 and 18 hours with combinations of Pectinex® 3XL and Celluclast 1.5L®, but for 27 h it was combinations of both these commercial solutions with XynA. The contribution of XynA was significant as C20P60X20 displayed the second highest reducing sugar production of 1.521 mg/mL, at 36 h from 12.875 μg/mL of protein, which was the second lowest protein loading. It was also shown that certain enzyme combinations, such as Pectinex® 3XL, Celluclast 1.5L® and XynA, did not generate synergy when combined in solution at the initial stages of hydrolysis, and instead generated a form of competition called anti-synergy. This was due to Pectinex® 3XL which had anti-synergy relationships in select combinations with the other enzyme solutions assayed. It was also observed that the degree of synergy and specific activity for a combination changed over time. Some solutions displayed the highest levels of synergy at the commencement of hydrolysis, namely Celluclast 1.5L®, ManA and XynA. Other combinations exhibited the highest levels of synergy at the end of the assay period, such as Pectinex® 3XL and Celluclast 1.5L®. Whether greater synergy was generated at the start or end of hydrolysis was a function of the stability of the enzymes in solution and whether enzyme activity increased substrate accessibility or generated competition between enzymes in solution. Sequential synergy studies demonstrated an anti-synergy relationship between Pectinex® 3XL and XynA or ManA, as well as Pectinex® 3 XL and Celluclast 1.5L®. It was found that under sequential synergy conditions with Pectinex® 3 XL, XynA and ManA, that anti-synergy could be negated and high degrees of synergy attained when the enzymes were added in specific loading orders and not inhibited by the presence of other active enzymes. The importance of loading order was demonstrated under sequential synergy conditions when XynA was added before ManA followed by Pectinex® 3 XL, which increased the activity and synergy of the solution by 50%. This equates to a 60% increase in reducing sugar release from the same concentrations of enzymes and emphasises the importance of removing anti-synergy relationships from combinations of enzymes. It can be concluded that a C20P60X20 combination (based on activity) can both synergistically increase the reducing sugar production and lower the protein loading required for pineapple pomace hydrolysis. This study also highlights the importance of reducing anti-synergy in customised enzyme cocktails and how sequential synergy can demonstrate the order in which a lignocellulosic waste is degraded.
- Full Text:
Linking satellite and point micrometeorological data to estimate : distributed evapotranspiration modelling based on MODIS LAI, Penman-Monteith and functional convergence theory
- Authors: Weideman, Craig Ivan
- Date: 2014
- Subjects: Plants -- Water requirements -- South Africa , Evaporation (Meteorology) -- Measurement , Satellite meteorology , Micrometeorology , Evapotranspiration , MODIS (Spectroradiometer)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4779 , http://hdl.handle.net/10962/d1012078 , Plants -- Water requirements -- South Africa , Evaporation (Meteorology) -- Measurement , Satellite meteorology , Micrometeorology , Evapotranspiration , MODIS (Spectroradiometer)
- Description: Recent advances in satellite sensor technology and micrometeorological instrumentation for water flux measurement, coupled with the expansion of automatic weather station networks that provide routine measurements of near-surface climate variables, present new opportunities for combining satellite and ground-based instrumentation to obtain distributed estimates of vegetation water use over wide areas in South Africa. In this study, a novel approach is tested, which uses satellite leaf area index (LAI) data retrieved by the Moderate Resolution Imaging Spectroradiometer (MODIS) to inform the FAO-56 Penman-Monteith equation for calculating reference evaporation (ET₀) of vegetation phenological activity. The model (ETMODIS) was validated at four sites in three different ecosystems across the country, including semi-arid savanna near Skukuza, mixed community grassland at Bellevue, near Pietermaritzburg, and Groenkop, a mixed evergreen indigenous forest near George, to determine potential for application over wider areas of the South African land surface towards meeting water resource management objectives. At Skukuza, evaluated against 170 days of flux data measured at a permanent eddy covariance (EC) flux tower in 2007, the model (ETMODIS) predicted 194.8 mm evapotranspiration relative to 148.9 mm measured fluxes, an overestimate of 31.7 %, (r² = 0.67). At an adjacent site, evaluated against flux data measured on two discrete periods of seven and eight days in February and May of 2005 using a large aperture scintillometer (SLS), ETMODIS predicted 27.4 mm and 6.7 mm evapotranspiration respectively, relative to measured fluxes of 32.5 and 8.2 mm, underestimates of 15.7 % and 18.3 % in each case (r² = 0.67 and 0.34, respectively). At Bellevue, evaluated against 235 days of evapotranspiration data measured using a surface layer scintillometer (SLS) in 2003, ETMODIS predicted 266.9 mm evapotranspiration relative to 460.2 mm measured fluxes, an underestimate of 42 % (r² = 0.67). At Groenkop, evaluated against data measured using a SLS over three discrete periods of four, seven and seven days in February, June and September/October respectively, ETMODIS predicted 9.7 mm, 10.3 mm and 17.0 mm evapotranspiration, relative to measured fluxes of 10.9 mm, 14.6 mm and 23. 9 mm, underestimates of 22.4 %, 11.2 % and 24.1 % in each case (r² = 0.98, 0.43 and 0.80, respectively). Total measured evapotranspiration exceeded total modelled evapotranspiration in all cases, with the exception of the flux tower site at Skukuza, where evapotranspiration was overestimated by ETMODIS by 31.7 % relative to measured (EC) values for the 170 days in 2007 where corresponding modelled and measured data were available. The most significant differences in measured versus predicted data were recorded at the Skukuza flux tower site in 2007 (31.7 % overestimate), and the Bellevue SLS flux site in 2003 (42 % underestimate); coefficients of determination, a measure of the extent to which modelled data are able to explain observed data at validation periods, with just two exceptions, were within a range of 0.67 – 0.98. Several sources of error and uncertainty were identified, relating predominantly to uncertainties in measured flux data used to evaluate ETMODIS, uncertainties in MODIS LAI submitted to ETMODIS, and uncertainties in ETMODIS itself, including model assumptions, and specific uncertainties relating to various inputs; further application of the model is required to test these uncertainties however, and establish confidence limits in performance. Nevertheless, the results of this study suggest that the technique is generally able to produce estimates of vegetation water use to within reasonably close approximations of measurements acquired using micrometeorological instruments, with r² values within the range of other peer-reviewed satellite remote sensing-based approaches.
- Full Text:
- Authors: Weideman, Craig Ivan
- Date: 2014
- Subjects: Plants -- Water requirements -- South Africa , Evaporation (Meteorology) -- Measurement , Satellite meteorology , Micrometeorology , Evapotranspiration , MODIS (Spectroradiometer)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4779 , http://hdl.handle.net/10962/d1012078 , Plants -- Water requirements -- South Africa , Evaporation (Meteorology) -- Measurement , Satellite meteorology , Micrometeorology , Evapotranspiration , MODIS (Spectroradiometer)
- Description: Recent advances in satellite sensor technology and micrometeorological instrumentation for water flux measurement, coupled with the expansion of automatic weather station networks that provide routine measurements of near-surface climate variables, present new opportunities for combining satellite and ground-based instrumentation to obtain distributed estimates of vegetation water use over wide areas in South Africa. In this study, a novel approach is tested, which uses satellite leaf area index (LAI) data retrieved by the Moderate Resolution Imaging Spectroradiometer (MODIS) to inform the FAO-56 Penman-Monteith equation for calculating reference evaporation (ET₀) of vegetation phenological activity. The model (ETMODIS) was validated at four sites in three different ecosystems across the country, including semi-arid savanna near Skukuza, mixed community grassland at Bellevue, near Pietermaritzburg, and Groenkop, a mixed evergreen indigenous forest near George, to determine potential for application over wider areas of the South African land surface towards meeting water resource management objectives. At Skukuza, evaluated against 170 days of flux data measured at a permanent eddy covariance (EC) flux tower in 2007, the model (ETMODIS) predicted 194.8 mm evapotranspiration relative to 148.9 mm measured fluxes, an overestimate of 31.7 %, (r² = 0.67). At an adjacent site, evaluated against flux data measured on two discrete periods of seven and eight days in February and May of 2005 using a large aperture scintillometer (SLS), ETMODIS predicted 27.4 mm and 6.7 mm evapotranspiration respectively, relative to measured fluxes of 32.5 and 8.2 mm, underestimates of 15.7 % and 18.3 % in each case (r² = 0.67 and 0.34, respectively). At Bellevue, evaluated against 235 days of evapotranspiration data measured using a surface layer scintillometer (SLS) in 2003, ETMODIS predicted 266.9 mm evapotranspiration relative to 460.2 mm measured fluxes, an underestimate of 42 % (r² = 0.67). At Groenkop, evaluated against data measured using a SLS over three discrete periods of four, seven and seven days in February, June and September/October respectively, ETMODIS predicted 9.7 mm, 10.3 mm and 17.0 mm evapotranspiration, relative to measured fluxes of 10.9 mm, 14.6 mm and 23. 9 mm, underestimates of 22.4 %, 11.2 % and 24.1 % in each case (r² = 0.98, 0.43 and 0.80, respectively). Total measured evapotranspiration exceeded total modelled evapotranspiration in all cases, with the exception of the flux tower site at Skukuza, where evapotranspiration was overestimated by ETMODIS by 31.7 % relative to measured (EC) values for the 170 days in 2007 where corresponding modelled and measured data were available. The most significant differences in measured versus predicted data were recorded at the Skukuza flux tower site in 2007 (31.7 % overestimate), and the Bellevue SLS flux site in 2003 (42 % underestimate); coefficients of determination, a measure of the extent to which modelled data are able to explain observed data at validation periods, with just two exceptions, were within a range of 0.67 – 0.98. Several sources of error and uncertainty were identified, relating predominantly to uncertainties in measured flux data used to evaluate ETMODIS, uncertainties in MODIS LAI submitted to ETMODIS, and uncertainties in ETMODIS itself, including model assumptions, and specific uncertainties relating to various inputs; further application of the model is required to test these uncertainties however, and establish confidence limits in performance. Nevertheless, the results of this study suggest that the technique is generally able to produce estimates of vegetation water use to within reasonably close approximations of measurements acquired using micrometeorological instruments, with r² values within the range of other peer-reviewed satellite remote sensing-based approaches.
- Full Text: