Recombinant expression, purification and in vitro interaction analysis of HOP and RhoC
- Authors: Vaaltyn, Michaelone Chantelle
- Date: 2016
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/64523 , vital:28555
- Description: Expected release date-May 2019
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Recommendations for the future of recreational fisheries to prepare the social‐ecological system to cope with change
- Authors: Arlinghaus, Robert , Cooke, Steven J , Sutton, S G , Danylchuk, A J , Potts, Warren M , Freire, K D M , Alós, J , Da Silva, E T , Cowx, Ian G , Van Anrooy, R
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125810 , vital:35819 , https://doi.10.1111/fme.12191
- Description: This paper presents conclusions and recommendations that emerged from the 7th World Recreational Fishing Conference (WRFC) held in Campinas, Brazil in September 2014. Based on the recognition of the immense social and economic importance of recreational fisheries coupled with weaknesses in robust information about these fisheries in many areas of the world, particularly in many economies in transition, it is recommended to increase effort to build effective governance arrangements and improve monitoring and assessment frameworks in data-poor situations. Moreover, there is a need to increase interdisciplinary studies that will foster a systematic understanding of recreational fisheries as complex adaptive social-ecological systems. To promote sustainable recreational fisheries on a global scale, it is recommended the detailed suggestions for governance and management outlined in the United Nations Food and Agricultural Organization Technical Guidelines for Responsible Fisheries: Recreational Fisheries are followed.
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Reimagining curriculum through a Bernsteinian lens: rethinking the canon in Political Science
- Authors: Clarence, Sherran
- Date: 2016
- Language: English
- Type: text , conference publication
- Identifier: http://hdl.handle.net/10962/66961 , vital:29006
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Remastered:
- Authors: Ntombela, N
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/146423 , vital:38524 , https://www.e-flux.com/announcements/49246/the-exhibitionist-journal-on-exhibition-making/
- Description: Exhibition abstract. Issue 12 scrutinizes our founding premise, that The Exhibitionist is a journal “by curators, for curators.” When we launched six years ago, this circuit of self-reflection seemed necessary, and enabled a conversation that was not available in other venues. But one effect of this focus, as we see it now, was a siloing-off of curatorial thinking from other parts of the field of exhibition making—which is, after all, based in collective forms of authorship.
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Report containing learning, reflection and evaluation based on social learning:
- Authors: Burt, Jane C , Wilson, Jessica , Copteros, Athina , Lotz-Sisitka, Heila , Pereira, Taryn , Mokoena, Samson , Munnik, Victor , Ngcozela, Thabang , Lusithi, Thabo
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/142005 , vital:38023 , ISBN WRC Report no K5/2313 Deliverable 7
- Description: This report forms the seventh deliverable in the NWRS2 citizen monitoring project and builds on the previous 6 deliverables, which include methodology for the project (Del 1), an assessment of civil society involvement in water policy (Del 2), an overview of the social learning approach and introduction to the case studies (Del 3), draft citizen monitoring guidelines (Del 4), an update on social learning to-date, including action plans (Del 5) and a report on a description and assessment of the case studies (Del 6). This report describes the last social learning module of the ‘Changing Practice’ course and highlights preliminary reflections on the learning that has taken place during this course. The report also describes the plans that were taken at the follow up research meeting. Finally we present the approach towards evaluating the role of social learning in the project as a whole.
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Responding to poverty in the light of the post-development debate: Some insights from the NGO Enda Graf Sahel
- Authors: Matthews, Sally
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142487 , vital:38084 , https://www.ajol.info/index.php/ad/article/view/135799
- Description: How can we take on board the many valuable insights of post-development theory without seeming to advocate indifference and inaction in the face of the misery that many people in the world experience daily? In this paper, I provide a partial response to this question. I begin by looking at some of the alternative strategies offered in post-development literature and set out to show that while there are several problems with these alternatives, to read post-development theory as advocating indifference or inaction is to read it uncharitably. Secondly, I draw on the experiences of the non-governmental organisation (NGO) Enda Graf Sahel in Dakar, Senegal to suggest some ways in which the insights of post-development theory, or some versions of post-development theory, can be taken into consideration without leading to inaction or indifference in the face of the suffering of those who occupy a less advantaged position in contemporary relations of power and privilege.
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Reviewing strategies in/for ESD policy engagement: Agency reclaimed
- Authors: Lotz-Sisitka, Heila
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/182483 , vital:43834 , xlink:href="https://doi.org/10.1080/00958964.2015.1113915"
- Description: In this response article, I draw on critical realist perspectives to engage with the argument put forward in Bengtsson's study, which sees agency as an ontological necessity for Education for Sustainable Development (ESD) policy engagement. Bengtsson supports a notion of the logic of contingent action over the logic of power as dominance, suggesting possibilities for agency and resistance. Although I do not in principle disagree with the agentive possibilities embedded in this aspect of the Bengtsson argument, it is the scope of the conceptualization thereof that I consider in this response. I start with considering the limitations of a Westphalian analysis of policy appropriations and agency for ESD, and argue that the Westphalian frame for policy analysis may be inadequate for capturing the significance of non-state actors and wider generative mechanisms such as informal normative structures, and private, economic power in the global political economy. Drawing on Fraser's (2008) concept of the transnational public sphere, I explore other potential possibilities for agency-centered appropriations or negations of, and/or resistance to ESD policy discourses, potentially expanding the agency-centered perspective referred to in Bengtsson's analysis and critique of policy making for ESD, or, at the very least, by offering a wider view of possibility for what he refers to as the ‘ineradicable moment of conflict, or antagonism.’ In particular, I broaden the notion of the transnational public sphere to be inclusive of Dussel's (1998) three concerns of transformation, namely; poverty and wealth inequality, environmental degradation, and narrow rationalities involving ongoing colonization of people, territories and resources. In doing this, I concur with Fraser, who suggests that the concept of the public sphere may well be “so thoroughly Westphalian in its deep conceptual structure as to be unsalvageable as a critical tool for theorizing the present” and suggest that public sphere thinking and associated conceptions of agency require expansion, which I offer from postcolonial and decolonization literature, critical realism, ontological experiences, and reflection on Environmental Education (EE) /ESD policy in the southern African region. Ultimately, I propose need for a more radical framework for EE/ ESD policy research that reaches beyond analyses of appropriations of policy within the Wesphalian state framework, and that moves beyond critiquing or seeking out resistance moments associated with the assumptions of trickle down effects from UN level policy, or analysis that is centered on the EE versus ESD debate. Such a framework requires a revitalized notion of agency involving commitment to collective, relational (including the socio-materially relational) and transgressive forms of agency for deep societal transformations all round. Overall, it seems that environmental education policy and praxis research conceptualized within a decolonizing transnational sphere frame appears to still be an open and as yet under-explored terrain.
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Reflections on Teaching Africa in South Africa:
- Authors: Matthews, Sally
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142235 , vital:38061 , DOI: 10.1111/1467-9256.12107
- Description: This article draws on the author’s experience of teaching African Studies to undergraduate South African students in order to reflect on some of the key challenges facing teachers of African Studies, both in South Africa and beyond. In particular, it discusses challenges relating to teaching a field as contested as African Studies, looking at whether teaching African alternatives to mainstream African politics is helpful and at whether and how one can teach Africa in a way that encourages and develops critical thinking. The article also explores how the racial politics of the context in which one teaches African Studies inevitably affects the way in which students engage with the content of the course. While the article discusses these issues in relation to the South African higher education context in particular, implications for other contexts are also highlighted.
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Rhodes University Graduation Ceremony 2016
- Authors: Rhodes University
- Date: 2016
- Language: English
- Type: text
- Identifier: vital:8150 , http://hdl.handle.net/10962/d1021288
- Description: Rhodes University Graduation Ceremonies 2016 [at] 1820 Settlers National Monument Thursday, 31 March at 10.00 & 17:00 [and] Friday, 1 April at 10:00; 14:30 & 18:30 [and] Saturday, 2 April at 10:00
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Russian wheat aphids: Breakfast, lunch, and supper. Feasting on small grains in South Africa
- Authors: Botha, Christiaan E J , Sacranie, S , Gallagher, Sean , Hill, Jaclyn M
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/69031 , vital:29374 , https://doi.org/10.1016/j.sajb.2016.12.006
- Description: The Russian Wheat Aphid (Diuraphis noxia, RWA) negatively impacts commercially grown barley and wheat in South Africa. Climate change, the attendant rise in [CO2], and the appearance of new RWA biotypes have the potential to induce severe crop yield loss in agriculturally important wheat and barley cultivars. This study presents data showing changes in relative aphid population numbers, concurrently with assessments of plant damage under controlled environmental conditions, under ambient and elevated (450 ppm) [CO2]. Extensive structural damage to the vascular tissue and disruption to the transport systems were revealed using light, fluorescence and electron microscopy. This, coupled with biotype population studies, demonstrated that RWA has the capacity to inflict severe, potentially permanent damage to vegetative small grain plants. Furthermore, some currently ‘resistant’ cultivars may well lose resistance as a direct result of increasing atmospheric [CO2]. A small (50 ppm) increase in atmospheric [CO2] may result in increased aphid population numbers, potentially serious plant damage and, by implication, a potentially negative impact on yield, as increased aphid density per plant leads to an accelerated disruption of the assimilate and transpiration transport pathways. These outcomes pose a direct threat to the commercial small grain industry of South Africa and by extension, to other small grain production areas elsewhere.
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Schools performing against the odds
- Authors: Niacker, Inbanathan , Grant, Carolyn , Pillay, Sivanandani
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/281126 , vital:55694 , xlink:href="https://doi.org/10.15700/saje.v36n4a1321"
- Description: There are many schools in developing countries which, despite the challenges they face, defy the odds and continue to perform at exceptionally high levels. We cast our gaze on one of these resilient schools in South Africa, and sought to learn about the leadership practices prevalent in this school and the enablements and constraints to the school leadership practice. Underpinned by a critical realist lens, and drawing on social realist theory, this case study of one school generated data through interviews, observation, document analysis and transect walks. The school principal, one head of department and two teachers, were selected as participants. The findings indicate that the school embraced an expansive form of teacher leadership comprising leadership within and beyond the classroom. Further, the structural, cultural and agential climate was receptive to the expansive leadership. We conclude that the professional capital of teachers, together with teachers serving as social actors rather than remaining primary agents, are key resources to change and transformation in an emerging economy.
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Screening of entomopathogenic fungi against citrus mealybug, Plannococcus citri (Hemiptera: Pseudococcidae)
- Authors: Chartier Fitzgerald, Veronique , Hill, Martin P , Moore, Sean D , Dames, Joanna F
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/407059 , vital:70333 , xlink:href="https://hdl.handle.net/10520/EJC195093"
- Description: Planococcus citri (citrus mealybug) is a common and damaging citrus crop pest which has proven difficult to control using conventional methods, such as chemical pesticides and insect growth regulators, particularly late in the citrus growing season. The virulence of two entomopathogenic fungal species was studied in laboratory bioassays against the crawlers and adults of P. citri. Isolates of Metarhizium anisopliae and Beauveria bassiana, collected from citrus orchards in the Eastern Cape Province in South Africa, were verified using and molecular techniques. Mealybug bioassays were performed in 24-well plates. Beauveria bassiana (GAR 17 B3) and M. anisopliae (FCM AR 23 B3) isolates both resulted in 67.5 % mortality of mealybug crawlers and B. bassiana (GB AR 23 13 3) resulted in 64 % crawler mortality with concentrations of 1 x 107 conidia/ml. These three isolates were further tested in multipledose bioassays to determine the median lethal concentration (LC50), which were 5.29 x 105conidia/ml for the M. anisopliae isolate (FCM AR 23 B3), 4.25 x 106 conidia/ml for B. bassiana (GAR 17 B3), and 6.65 x 107 conidia/ml B. bassiana (GB AR 23 13 3) for crawlers, respectively. The results of this study suggested that two isolates (M. anisopliae FCM AR 23 B3 and B. bassiana GAR 17 B3) showed potential for further development as biological control agents against citrus mealybug. Further research would be required to determine their ability to perform under field conditions.
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SEAmester – South Africa’s first class afloat
- Authors: Ansorge, Isabelle J , Brundrit, Geoff , Brundrit, Jean , Dorrington, Rosemary A , Fawcett, Sarah , Gammon, David , Henry, Tahlia , Hermes, Juliet , Hölscher, Beate , d’Hotman, Jethan , Meiklejohn, Ian , Morris, Tammy , Pinto, Izidine , Du Plessis, Marcel , Roman, Raymond , Saunders, Clinton , Shabangu, Fannie W , De Vos, Marc , Walker, David R , Louw, Gavin
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/65539 , vital:28808 , https://doi.org/10.17159/sajs.2016/a0171
- Description: publisher version , From Introduction: Marine science is a highly competitive environment. The need to improve the cohort of South African postgraduates, who would be recognised both nationally and internationally for their scientific excellence, is crucial. It is possible to attract students early on in their careers to this discipline via cutting-edge science, technology and unique field experiences. Through the engagement of students with real-life experiences such as SEAmester, universities supporting marine science postgraduate degree programmes can attract a sustainable throughput of numerically proficient students. By achieving a more quantitative and experienced input into our postgraduate degree programmes, we will, as a scientific community, greatly improve our long-term capabilities to accurately measure, model and predict the impacts of current climate change scenarios. The short-term goal is to attract and establish a cohort of proficient marine and atmospheric science graduates who will contribute to filling the capacity needs of South African marine science as a whole. The SEAmester programme, by involving researchers from across all the relevant disciplines and tertiary institutions, provides an opportunity to build a network of collaborative teaching within the marine field. In doing so, these researchers will foster and strengthen new and current collaborations between historically white and black universities (Figure 1). The long-term objective of SEAmester is to build critical mass within the marine sciences to ensure sustained growth of human capacity in marine science in South Africa – aligning closely with the current DST Research and Development strategies and the Operation Phakisa Oceans Economy initiative.
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Sentential negation in South African Sign Language: A case study
- Authors: De Barros, Courtney , Siebörger, Ian
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/385377 , vital:68013 , xlink:href=" https://hdl.handle.net/10520/EJC-4acb542a9"
- Description: As with other sign languages, South African Sign Language (SASL) expresses negation using both manual and non-manual features. In this case study, naturalistic data provided by two native signers of SASL are analysed to show the syntactic relationship between these two sets of features. Using a Principles and Parameters approach and Government and Binding Theory, we investigate the syntactic scope of negation in our SASL data. We observe that side-to-side headshake, as a non-manual feature, appears to be the chief clausal negator in SASL, with a clause-final manual negative particle, NOT, playing a secondary role. We describe the negative headshake as a featural affix which is base-generated in the head of NegP and triggers V-to- Neg raising. The negative particle NOT appears to be base-generated in the Specifier of NegP. Suggestions for further syntactic research on SASL are provided.
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Sex and Loneliness: Banele Khoza’s ‘Temporary Feelings
- Authors: Lila, Philiswa
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/147149 , vital:38597 , https://artthrob.co.za/2016/07/28/sex-and-loneliness-banele-khozas-temporary-feelings/
- Description: Banele Khoza grapples with short sharp emotions in his solo exhibition at the Pretoria Art Museum. Entitled ‘Temporary Feelings,’ the exhibition is very personal, yet critically engaging with issues of relationships, communication, social media and sexuality. He brings his vulnerability into the gallery space: what it means to be lonely, even though surrounded by people.
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Sha-1 and the strict avalanche criterion
- Authors: Motara, Yusuf, M , Irwin, Barry V W
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/429010 , vital:72553 , https://ieeexplore.ieee.org/abstract/document/7802926
- Description: The Strict Avalanche Criterion (SAC) is a measure of both confusion and diffusion, which are key properties of a cryptographic hash function. This work provides a working definition of the SAC, describes an experimental methodology that can be used to statistically evaluate whether a cryptographic hash meets the SAC, and uses this to investigate the degree to which compression function of the SHA-1 hash meets the SAC. The results (P 0.01) are heartening: SHA-1 closely tracks the SAC after the first 24 rounds, and demonstrates excellent properties of confusion and diffusion throughout.
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SIERRA: ERM and usage statistics
- Authors: Van der Walt, Wynand , Innovative User Group Southern Africa (IUGSA), 14th Annual Conference : 2016 : Vanderbijlpark, Gauteng
- Date: 2016
- Subjects: Library science
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/5611 , vital:20946
- Description: This paper outlines the processes involved in incorporating e-resources usages statistics into the ERM module within Sierra. The challenges and results of using the JR1 COUNTER statistics within the ERM module will be discussed.
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Soil microbial properties and apple tree performance under conventional and organic management
- Authors: Meyer, André Harold
- Date: 2016
- Subjects: Soil management South Africa Western Cape , Agricultural chemicals Environmental aspects , Soil microbiology South Africa Western Cape , Vesicular-arbuscular mycorrhizas , Enzymes Biotechnology , Apples Organic farming South Africa Western Cape
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/64545 , vital:28557
- Description: Conventional (CON) soil management that permits the use of agrochemicals is currently the most common form of management in Western Cape deciduous fruit orchards. There is increasing pressure to minimise or eliminate synthetic agrochemical usage due to its potentially harmful effect on the environment, particularly to non-target soil microorganisms, and to the functions and processes they perform or mediate. In apple orchards, organic (ORG) practices exclude the use of synthetic pesticides and herbicides making use instead of organic fertilisers and naturally derived products as defined by organic certification programs. ORG practices aim to improve nutrient availability, yield, and long-term orchard sustainability relative to CON orchard management practices. If ORG and CON orchard floor management practices affect orchard ecosystems differently, such differences should be measurable in terms of differences in microbiological parameters. In this thesis it is hypothesised that ORG practices would induce positive soil microbiological responses in Western Cape apple orchards relative to CON practices, and by inference general soil health and apple tree performance. To test this hypothesis a polyphasic approach was adopted. This involved measurement of soil microbial activities and functional diversities, by enzyme activity (using colorimetric assays) and carbon-substrate utilisation (using the BIOLOG™ system), respectively. With reference to the enzyme analyses, the performance of a literature-validated, enzyme-based soil health index was also tested. The analyses were supported by coarse-level comparisons of the magnitude of bacteria, fungi, actinobacteria and total heterotroph populations using traditional culturing techniques (dilution plating on growth media). The extent to which the microbial status differed between the applied ORG and CON treatments was thought likely to reflect such treatment-induced variables as soil nutrient status, apple tree nutritional response, tree growth and yield, all of which were determined. Because the root systems of deciduous fruit trees commonly extend to depths >60 cm in well-prepared soils, microbial enzyme activities in the soil depth intervals corresponding to the lower rootzone, were also investigated. This research was carried out in a randomized field trial. Finally, to gain a broader understanding of the effects of contrasting soil management systems on soil microbiology under a greater variety of environmental conditions, arbuscular mycorrhizal (AM) fungal dynamics were explored in a survey of commercial apple orchards. These orchards were selected to span the range of environmental conditions that occur in the apple production areas of the Western Cape. Orchard soils under ORG management promoted richer microbial ecosystems, and appeared to be better able to sustain community metabolic diversity and, by inference, the functions mediated by soil microbial communities, than those under CON management. This implies that ORG approaches possibly afford a better option to sustain critical ecosystem functions than CON management. This possibly explains why use of straw mulches and compost in accordance with ORG practices, compared with CON practices, promoted β-glucosidase, acid phosphatase and urease activities rather than affecting the abundance of the micro-organisms that produce these enzymes. Enzyme activities in the 0–30 cm soil intervals were also more effectively promoted by ORG than CON practices, although no differences were observed at lower depth intervals. ORG practices promoted functional AM associations more effectively than CON practices, but the abundance of glomalin, a beneficial by-product of AM fungi, was unaffected. The greater enzyme activities and higher root colonisation levels in the ORG treatments probably contributed to improved nutritional effects that caused greater vegetative growth, but lower yields, in the ORG treatments. Yield suppression was conceivably due to excessive vegetative growth induced by oversupply of compost and the mineral nutrients contained therein. The survey of Western Cape apple orchards suggested that neither glomalin nor root colonisation bore any specific relationship to production area, cultivation practice, scion x rootstock combination, or, in the case of root colonisation, with any chemical parameters. However, the effect of season on glomalin was conclusively shown, being higher in summer than in spring, as was the lack of any effect of year on glomalin and root colonisation. Collectively, these results showed that ORG soil management promote soil microbiology, soil nutrient status, and apple tree performance compared to CON management. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2016
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Song analysis of South African pygmy bladder cicadas (Hemiptera: Cicadidae: Cicadettinae: Tettigomyiini).
- Authors: Sanborn, Allen F , Phillips, Polly K F , Villet, Martin H
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442319 , vital:73975 , https://doi.org/10.1093/aesa/saw024
- Description: The callings songs of five species from three genera of South African pygmy bladder cicadas are analyzed. The call of each species has a distinct temporal pattern and frequency spectrum. The songs are of significantly lower frequency than would be predicted based on body mass or body length. Comparison of bladder cicada calls from Australia and South Africa show similar lower than predicted frequencies in species of independent evolutionary origin. The inflated abdomen found in these cicadas appears to be a convergent adaptation to permit more efficient song production at lower carrier frequencies that increase the distance the songs will travel.
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Spectroscopic investigations and theoretical calculations of DABCO induced xanthene bridged self-assembled zinc (II) porphyrin dimer
- Authors: Xu, Li , Huang, Tingting , Liang, Xu , Mack, John , Harris, Jessica , Nyokong, Tebello , Li, Minzhi , Zhu, Weihua
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/240732 , vital:50866 , xlink:href="https://doi.org/10.1142/S1088424616500231"
- Description: An in-depth study of the electronic structure of a 1,4-diazabicyclo[2.2.2]octane (DABCO) induced molecular self-assembled xanthene-bridged and amide-bonded porphyrin dimer is reported. Density functional theory (DFT) and time-dependent DFT (TD-DFT) calculations are used to identify trends in the optical spectroscopic properties. B3LYP geometry optimization predicts the formation of an almost perfectly eclipsed structure with respect to the two porphyrin rings with the analogous pyrrole nitrogens separated by 7.7–8.1 Å. The observed distinctive derivative-shaped band morphology of the pseudo-Faraday-A11 terms in the MCD spectra has been used to identify the main electronic Q and B-bands and to validate the TD-DFT calculations. The absence of a discernible splitting of the redox steps or a quenching of the fluorescence demonstrates that there is no significant exciton coupling between the two porphyrin rings.
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