Song analysis of South African pygmy bladder cicadas (Hemiptera: Cicadidae: Cicadettinae: Tettigomyiini).
- Sanborn, Allen F, Phillips, Polly K F, Villet, Martin H
- Authors: Sanborn, Allen F , Phillips, Polly K F , Villet, Martin H
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442319 , vital:73975 , https://doi.org/10.1093/aesa/saw024
- Description: The callings songs of five species from three genera of South African pygmy bladder cicadas are analyzed. The call of each species has a distinct temporal pattern and frequency spectrum. The songs are of significantly lower frequency than would be predicted based on body mass or body length. Comparison of bladder cicada calls from Australia and South Africa show similar lower than predicted frequencies in species of independent evolutionary origin. The inflated abdomen found in these cicadas appears to be a convergent adaptation to permit more efficient song production at lower carrier frequencies that increase the distance the songs will travel.
- Full Text:
- Authors: Sanborn, Allen F , Phillips, Polly K F , Villet, Martin H
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442319 , vital:73975 , https://doi.org/10.1093/aesa/saw024
- Description: The callings songs of five species from three genera of South African pygmy bladder cicadas are analyzed. The call of each species has a distinct temporal pattern and frequency spectrum. The songs are of significantly lower frequency than would be predicted based on body mass or body length. Comparison of bladder cicada calls from Australia and South Africa show similar lower than predicted frequencies in species of independent evolutionary origin. The inflated abdomen found in these cicadas appears to be a convergent adaptation to permit more efficient song production at lower carrier frequencies that increase the distance the songs will travel.
- Full Text:
South Africa in the Antarctic Circumnavigation Expedition: a multi-institutional and interdisciplinary scientific project
- Halo, Issufo, Dorrington, Rosemary A, Bornman, Thomas G, De Villiers, Stephanie, Fawcett, Sarah
- Authors: Halo, Issufo , Dorrington, Rosemary A , Bornman, Thomas G , De Villiers, Stephanie , Fawcett, Sarah
- Date: 2016
- Language: English
- Type: article
- Identifier: http://hdl.handle.net/10962/65428 , vital:28790 , https://doi.org/10.17159/sajs.2016/a0173
- Description: publisher version , The polar regions are more critically affected by climate change than any other region on our planet.1,2 On the Antarctic continent and in its surrounding oceans, the effects of climate change are likely to be dramatic,3 and include largescale catastrophic ice melt, loss of habitat and biodiversity, and global sea level rise. The ‘Southern Ocean’ refers to the region where Atlantic, Indian and Pacific Ocean waters come together to encircle Antarctica. These waters connect the different ocean basins by linking the shallow and deep limbs of the global ocean current system (‘overturning circulation’) and play a critical role in storing and distributing heat and carbon dioxide (CO2 ). The Southern Ocean thus regulates not only the climate of the Antarctic, but of the entire earth system.1,4 By extension, the capacity of the global ocean to ameliorate earth’s changing climate is strongly controlled by the Southern Ocean. Marine phytoplankton (microscopic plants inhabiting the sunlit upper ocean) convert CO2 (an inorganic form of carbon) dissolved in surface waters into organic carbon through photosynthesis. This organic carbon fuels upper trophic levels such as fish, mammals and birds, and a portion sinks into the deep ocean where it remains stored for hundreds to thousands of years. This mechanism, which lowers the atmospheric concentration of CO2 , is termed the ‘biological pump’.5 The efficiency of the global ocean’s biological pump is currently limited by the Southern Ocean, where the macronutrients (nitrate and phosphate) required for photosynthesis are never fully consumed in surface waters. In theory, increased consumption of these nutrients could drive higher organic carbon removal to the deep ocean, enhancing the oceanic uptake of atmospheric CO2 . Indeed, more complete consumption of Southern Ocean nutrients is a leading hypothesis for the decrease in atmospheric CO2 that characterised the ice ages.6 Despite the global importance of the Southern Ocean, knowledge of the controls on and interactions among the physical, chemical and biological processes operating in Antarctic ecosystems is limited, largely because of a scarcity of in-situ observational data, compounded by the challenge of integrating siloed scientific fields. Given predictions that diverse aspects of Southern Ocean physics and carbon biogeochemistry are likely to change in the coming decades, a transdisciplinary approach to studying Antarctic systems is critical.
- Full Text:
- Authors: Halo, Issufo , Dorrington, Rosemary A , Bornman, Thomas G , De Villiers, Stephanie , Fawcett, Sarah
- Date: 2016
- Language: English
- Type: article
- Identifier: http://hdl.handle.net/10962/65428 , vital:28790 , https://doi.org/10.17159/sajs.2016/a0173
- Description: publisher version , The polar regions are more critically affected by climate change than any other region on our planet.1,2 On the Antarctic continent and in its surrounding oceans, the effects of climate change are likely to be dramatic,3 and include largescale catastrophic ice melt, loss of habitat and biodiversity, and global sea level rise. The ‘Southern Ocean’ refers to the region where Atlantic, Indian and Pacific Ocean waters come together to encircle Antarctica. These waters connect the different ocean basins by linking the shallow and deep limbs of the global ocean current system (‘overturning circulation’) and play a critical role in storing and distributing heat and carbon dioxide (CO2 ). The Southern Ocean thus regulates not only the climate of the Antarctic, but of the entire earth system.1,4 By extension, the capacity of the global ocean to ameliorate earth’s changing climate is strongly controlled by the Southern Ocean. Marine phytoplankton (microscopic plants inhabiting the sunlit upper ocean) convert CO2 (an inorganic form of carbon) dissolved in surface waters into organic carbon through photosynthesis. This organic carbon fuels upper trophic levels such as fish, mammals and birds, and a portion sinks into the deep ocean where it remains stored for hundreds to thousands of years. This mechanism, which lowers the atmospheric concentration of CO2 , is termed the ‘biological pump’.5 The efficiency of the global ocean’s biological pump is currently limited by the Southern Ocean, where the macronutrients (nitrate and phosphate) required for photosynthesis are never fully consumed in surface waters. In theory, increased consumption of these nutrients could drive higher organic carbon removal to the deep ocean, enhancing the oceanic uptake of atmospheric CO2 . Indeed, more complete consumption of Southern Ocean nutrients is a leading hypothesis for the decrease in atmospheric CO2 that characterised the ice ages.6 Despite the global importance of the Southern Ocean, knowledge of the controls on and interactions among the physical, chemical and biological processes operating in Antarctic ecosystems is limited, largely because of a scarcity of in-situ observational data, compounded by the challenge of integrating siloed scientific fields. Given predictions that diverse aspects of Southern Ocean physics and carbon biogeochemistry are likely to change in the coming decades, a transdisciplinary approach to studying Antarctic systems is critical.
- Full Text:
Spectroscopic investigations and theoretical calculations of DABCO induced xanthene bridged self-assembled zinc (II) porphyrin dimer
- Xu, Li, Huang, Tingting, Liang, Xu, Mack, John, Harris, Jessica, Nyokong, Tebello, Li, Minzhi, Zhu, Weihua
- Authors: Xu, Li , Huang, Tingting , Liang, Xu , Mack, John , Harris, Jessica , Nyokong, Tebello , Li, Minzhi , Zhu, Weihua
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/240732 , vital:50866 , xlink:href="https://doi.org/10.1142/S1088424616500231"
- Description: An in-depth study of the electronic structure of a 1,4-diazabicyclo[2.2.2]octane (DABCO) induced molecular self-assembled xanthene-bridged and amide-bonded porphyrin dimer is reported. Density functional theory (DFT) and time-dependent DFT (TD-DFT) calculations are used to identify trends in the optical spectroscopic properties. B3LYP geometry optimization predicts the formation of an almost perfectly eclipsed structure with respect to the two porphyrin rings with the analogous pyrrole nitrogens separated by 7.7–8.1 Å. The observed distinctive derivative-shaped band morphology of the pseudo-Faraday-A11 terms in the MCD spectra has been used to identify the main electronic Q and B-bands and to validate the TD-DFT calculations. The absence of a discernible splitting of the redox steps or a quenching of the fluorescence demonstrates that there is no significant exciton coupling between the two porphyrin rings.
- Full Text:
- Authors: Xu, Li , Huang, Tingting , Liang, Xu , Mack, John , Harris, Jessica , Nyokong, Tebello , Li, Minzhi , Zhu, Weihua
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/240732 , vital:50866 , xlink:href="https://doi.org/10.1142/S1088424616500231"
- Description: An in-depth study of the electronic structure of a 1,4-diazabicyclo[2.2.2]octane (DABCO) induced molecular self-assembled xanthene-bridged and amide-bonded porphyrin dimer is reported. Density functional theory (DFT) and time-dependent DFT (TD-DFT) calculations are used to identify trends in the optical spectroscopic properties. B3LYP geometry optimization predicts the formation of an almost perfectly eclipsed structure with respect to the two porphyrin rings with the analogous pyrrole nitrogens separated by 7.7–8.1 Å. The observed distinctive derivative-shaped band morphology of the pseudo-Faraday-A11 terms in the MCD spectra has been used to identify the main electronic Q and B-bands and to validate the TD-DFT calculations. The absence of a discernible splitting of the redox steps or a quenching of the fluorescence demonstrates that there is no significant exciton coupling between the two porphyrin rings.
- Full Text:
Sperm ultrastructure and spermatodesm morphology of the spittle bug Locris transversa (Thunberg 1822)(Hemiptera: Cercopidae)
- Hodgson, Alan N, Ridgeway, Jaryd A, Villet, Martin H
- Authors: Hodgson, Alan N , Ridgeway, Jaryd A , Villet, Martin H
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442333 , vital:73976 , https://doi.org/10.1080/07924259.2016.1157104
- Description: The structure of the spermatozoon and spermatodesm of the spittlebug Locris transversa (Thunberg 1822) was investigated by light and transmission electron microscopy. Males produced only one size class of sperm, which was 93–106 μm long. During spermatogenesis groups of spermatozoa are arranged around, and attached by their acrosomes to, a small central extracellular matrix to form a ball-shaped spermatodesm. Spermatodesmata were found in the testis, vas deferens and seminal vesicle of males and spermatheca of females. The sperm are filiform, each consisting of a ~15-μm-long head containing an anteriorly positioned conical ~2-μm-long acrosome and ~13-μm-long nucleus, and a midpiece and tail with a 9+9+2 axoneme. The acrosome, which has two posterior extensions that lie along one side of the anterior region of the nucleus, contains longitudinally orientated microfilaments. The nucleus has two unequal anterior extensions, whereas posteriorly it is flattened laterally to accommodate a putative centriolar adjunct and anterior ends of the two mitochondrial derivatives. The basal body lies posterior to the nucleus. The mitochondrial derivatives are elongated and extend for almost the entire length of the tail. The tail has glycogen within its centre, and towards its terminal end large deposits of glycogen surround the mitochondrial derivatives and axoneme. Compared to other members of the Cicadomorpha, the spermatozoa and spermatodesms of cercopids so far studied have some structural features in common with cicadellids and others in common with cicadas; therefore, if sperm features are to be useful in phylogenetic studies of cicadomorphs, attention must be given to examining key taxa to establish which character states are plesiomorphic or apomorphic.
- Full Text:
- Authors: Hodgson, Alan N , Ridgeway, Jaryd A , Villet, Martin H
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442333 , vital:73976 , https://doi.org/10.1080/07924259.2016.1157104
- Description: The structure of the spermatozoon and spermatodesm of the spittlebug Locris transversa (Thunberg 1822) was investigated by light and transmission electron microscopy. Males produced only one size class of sperm, which was 93–106 μm long. During spermatogenesis groups of spermatozoa are arranged around, and attached by their acrosomes to, a small central extracellular matrix to form a ball-shaped spermatodesm. Spermatodesmata were found in the testis, vas deferens and seminal vesicle of males and spermatheca of females. The sperm are filiform, each consisting of a ~15-μm-long head containing an anteriorly positioned conical ~2-μm-long acrosome and ~13-μm-long nucleus, and a midpiece and tail with a 9+9+2 axoneme. The acrosome, which has two posterior extensions that lie along one side of the anterior region of the nucleus, contains longitudinally orientated microfilaments. The nucleus has two unequal anterior extensions, whereas posteriorly it is flattened laterally to accommodate a putative centriolar adjunct and anterior ends of the two mitochondrial derivatives. The basal body lies posterior to the nucleus. The mitochondrial derivatives are elongated and extend for almost the entire length of the tail. The tail has glycogen within its centre, and towards its terminal end large deposits of glycogen surround the mitochondrial derivatives and axoneme. Compared to other members of the Cicadomorpha, the spermatozoa and spermatodesms of cercopids so far studied have some structural features in common with cicadellids and others in common with cicadas; therefore, if sperm features are to be useful in phylogenetic studies of cicadomorphs, attention must be given to examining key taxa to establish which character states are plesiomorphic or apomorphic.
- Full Text:
Sport consumption patterns in the Eastern Cape: cricket spectators as sporting univores or omnivores
- Brock, Kelcey, Fraser, Gavin C G, Botha, Ferdi
- Authors: Brock, Kelcey , Fraser, Gavin C G , Botha, Ferdi
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/69362 , vital:29508 , https://doi.org/10.4102/jef.v9i3.64
- Description: Since its inception, consumption behaviour theory has developed to account for the important social aspects that underpin or at least to some extent explain consumer behaviour. Empirical studies on consumption behaviour of cultural activities, entertainment and sport have used Bourdieu’s (1984) omnivore/univore theory to investigate consumption of leisure activities. The aim of this study is to investigate whether South African cricket spectators are sporting omnivores or univores. The study was conducted among cricket spectators in the Eastern Cape at four limited overs cricket matches in the 2012/2013 cricket season. The results indicate that consumption behaviour of sport predominantly differs on the grounds of education and race. This suggests that there are aspects of social connotations underpinning sports consumption behaviour within South Africa.
- Full Text:
Sport consumption patterns in the Eastern Cape: cricket spectators as sporting univores or omnivores
- Authors: Brock, Kelcey , Fraser, Gavin C G , Botha, Ferdi
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/69362 , vital:29508 , https://doi.org/10.4102/jef.v9i3.64
- Description: Since its inception, consumption behaviour theory has developed to account for the important social aspects that underpin or at least to some extent explain consumer behaviour. Empirical studies on consumption behaviour of cultural activities, entertainment and sport have used Bourdieu’s (1984) omnivore/univore theory to investigate consumption of leisure activities. The aim of this study is to investigate whether South African cricket spectators are sporting omnivores or univores. The study was conducted among cricket spectators in the Eastern Cape at four limited overs cricket matches in the 2012/2013 cricket season. The results indicate that consumption behaviour of sport predominantly differs on the grounds of education and race. This suggests that there are aspects of social connotations underpinning sports consumption behaviour within South Africa.
- Full Text:
Spotlight on the Art of Darkness: The Kin Artstudio
- Authors: Tshilumba Mukendi, J S
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/146434 , vital:38525 , https://artafricamagazine.org/magazine-archive/?v=e4dd286dc7d7
- Description: Issue December 2016. What Really Matters.
- Full Text:
- Authors: Tshilumba Mukendi, J S
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/146434 , vital:38525 , https://artafricamagazine.org/magazine-archive/?v=e4dd286dc7d7
- Description: Issue December 2016. What Really Matters.
- Full Text:
Stigma resistance in online childfree communities: The limitations of choice rhetoric
- Morison, Tracy, Macleod, Catriona I, Lynch, Ingrid, Shivakumar, Seemanthini T
- Authors: Morison, Tracy , Macleod, Catriona I , Lynch, Ingrid , Shivakumar, Seemanthini T
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/446299 , vital:74488 , xlink:href="https://doi.org/10.1177/0361684315603657"
- Description: People who are voluntarily childless, or ‘‘childfree,’’ face considerable stigma. Researchers have begun to explore how these individuals respond to stigma, usually focusing on interpersonal stigma management strategies. We explored participants’ responses to stigma in a way that is cognisant of broader social norms and gender power relations. Using a feminist discursive psychology framework, we analysed women’s and men’s computer-assisted communication about their childfree status. Our analysis draws attention to ‘‘identity work’’ in the context of stigma. We show how the strategic use of ‘‘choice’’ rhetoric allowed participants to avoid stigmatised identities and was used in two contradictory ways. On the one hand, participants drew on a ‘‘childfree-by-choice script,’’ which enabled them to hold a positive identity of themselves as autonomous, rational, and responsible decision makers. On the other hand, they mobilised a ‘‘disavowal of choice script’’ that allowed a person who is unable to choose childlessness (for various reasons) to hold a blameless identity regarding deviation from the norm of parenthood. We demonstrate how choice rhetoric allowed participants to resist stigma and challenge pronatalism to some extent; we discuss the political potential of these scripts for reproductive freedom.
- Full Text:
- Authors: Morison, Tracy , Macleod, Catriona I , Lynch, Ingrid , Shivakumar, Seemanthini T
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/446299 , vital:74488 , xlink:href="https://doi.org/10.1177/0361684315603657"
- Description: People who are voluntarily childless, or ‘‘childfree,’’ face considerable stigma. Researchers have begun to explore how these individuals respond to stigma, usually focusing on interpersonal stigma management strategies. We explored participants’ responses to stigma in a way that is cognisant of broader social norms and gender power relations. Using a feminist discursive psychology framework, we analysed women’s and men’s computer-assisted communication about their childfree status. Our analysis draws attention to ‘‘identity work’’ in the context of stigma. We show how the strategic use of ‘‘choice’’ rhetoric allowed participants to avoid stigmatised identities and was used in two contradictory ways. On the one hand, participants drew on a ‘‘childfree-by-choice script,’’ which enabled them to hold a positive identity of themselves as autonomous, rational, and responsible decision makers. On the other hand, they mobilised a ‘‘disavowal of choice script’’ that allowed a person who is unable to choose childlessness (for various reasons) to hold a blameless identity regarding deviation from the norm of parenthood. We demonstrate how choice rhetoric allowed participants to resist stigma and challenge pronatalism to some extent; we discuss the political potential of these scripts for reproductive freedom.
- Full Text:
Structure based docking and molecular dynamic studies of plasmodial cysteine proteases against a South African natural compound and its analogs:
- Musyoka, Thommas M, Kanzi, Aquillah M, Lobb, Kevin A, Tastan Bishop, Özlem
- Authors: Musyoka, Thommas M , Kanzi, Aquillah M , Lobb, Kevin A , Tastan Bishop, Özlem
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148027 , vital:38703 , DOI: 10.1038/srep23690
- Description: Identification of potential drug targets as well as development of novel antimalarial chemotherapies with unique mode of actions due to drug resistance by Plasmodium parasites are inevitable. Falcipains (falcipain-2 and falcipain-3) of Plasmodium falciparum, which catalyse the haemoglobin degradation process, are validated drug targets. Previous attempts to develop peptide based drugs against these enzymes have been futile due to the poor pharmacological profiles and susceptibility to degradation by host enzymes. This study aimed to identify potential non-peptide inhibitors against falcipains and their homologs from other Plasmodium species.
- Full Text:
- Authors: Musyoka, Thommas M , Kanzi, Aquillah M , Lobb, Kevin A , Tastan Bishop, Özlem
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148027 , vital:38703 , DOI: 10.1038/srep23690
- Description: Identification of potential drug targets as well as development of novel antimalarial chemotherapies with unique mode of actions due to drug resistance by Plasmodium parasites are inevitable. Falcipains (falcipain-2 and falcipain-3) of Plasmodium falciparum, which catalyse the haemoglobin degradation process, are validated drug targets. Previous attempts to develop peptide based drugs against these enzymes have been futile due to the poor pharmacological profiles and susceptibility to degradation by host enzymes. This study aimed to identify potential non-peptide inhibitors against falcipains and their homologs from other Plasmodium species.
- Full Text:
Subcellular localisation of Theiler's murine encephalomyelitis virus (TMEV) capsid subunit VP1 vis-á-vis host protein Hsp90:
- Ross, Caroline J, Upfold, Nicole, Luke, Garry A, Tastan Bishop, Özlem, Knox, Caroline M
- Authors: Ross, Caroline J , Upfold, Nicole , Luke, Garry A , Tastan Bishop, Özlem , Knox, Caroline M
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148016 , vital:38702 , DOI: 10.1016/j.virusres.2016.06.003
- Description: The VP1 subunit of the picornavirus capsid is the major antigenic determinant and mediates host cell attachment and virus entry. To investigate the localisation of Theiler's murine encephalomyelitis virus (TMEV) VP1 during infection, a bioinformatics approach was used to predict a surface-exposed, linear epitope region of the protein for subsequent expression and purification. This region, comprising the N-terminal 112 amino acids of the protein, was then used for rabbit immunisation, and the resultant polyclonal antibodies were able to recognise full length VP1 in infected cell lysates by Western blot. Following optimisation, the antibodies were used to investigate the localisation of VP1 in relation to Hsp90 in infected cells by indirect immunofluorescence and confocal microscopy.
- Full Text:
- Authors: Ross, Caroline J , Upfold, Nicole , Luke, Garry A , Tastan Bishop, Özlem , Knox, Caroline M
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148016 , vital:38702 , DOI: 10.1016/j.virusres.2016.06.003
- Description: The VP1 subunit of the picornavirus capsid is the major antigenic determinant and mediates host cell attachment and virus entry. To investigate the localisation of Theiler's murine encephalomyelitis virus (TMEV) VP1 during infection, a bioinformatics approach was used to predict a surface-exposed, linear epitope region of the protein for subsequent expression and purification. This region, comprising the N-terminal 112 amino acids of the protein, was then used for rabbit immunisation, and the resultant polyclonal antibodies were able to recognise full length VP1 in infected cell lysates by Western blot. Following optimisation, the antibodies were used to investigate the localisation of VP1 in relation to Hsp90 in infected cells by indirect immunofluorescence and confocal microscopy.
- Full Text:
Supplementary Material Synthesis and biological evaluation of (E)-cinnamic acid,(E)-2-styrylthiazole and (E)-2-[2-(naphthalen-1-yl) vinyl] thiazole derivatives
- Olawode, Emmanuel O, Tandlich, Roman, Prinsloo, Earl, Isaacs, Michelle, Hoppe, Heinrich C, Seldon, Ronnett, Warner, Digby F, Steenkamp, Vanessa, Kaye, Perry T
- Authors: Olawode, Emmanuel O , Tandlich, Roman , Prinsloo, Earl , Isaacs, Michelle , Hoppe, Heinrich C , Seldon, Ronnett , Warner, Digby F , Steenkamp, Vanessa , Kaye, Perry T
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/431778 , vital:72803 , xlink:href=" https://www.arkat-usa.org/get-file/59868/"
- Description: The screening was conducted using multi-well plates which are suited for HeLa cells in the log phase of growth with final cell density > 10 cells/cm. Each experiment normally includes a blank control, containing medium without the cells.28,45 Non-contaminated HeLa cells (6.57 x 105 cells per well) in media were allowed to grow in the incubator under an atmosphere of 5% CO2 at 37 0C for 24 h. To each well was dispensed 200 µL of HeLa culture, containing 6.57 x 105 cells under LabEAir laminar flow hood (Vivid Air, South Africa); 20 µL of resazurin dye (Sigma TOX-8) and test compound (50 µL) were added, which were then incubated in the presence of 5% CO2 at 37 0C for 24 hours in a shaker, to enhance the distribution of the dye. The absorbance of each well was measured with Bio-tek Power Wave X fluorometer (Beijing, China), and increases in fluorescence was monitored at a wavelength of 590 nm, using an excitation wavelength of 560 nm.
- Full Text:
- Authors: Olawode, Emmanuel O , Tandlich, Roman , Prinsloo, Earl , Isaacs, Michelle , Hoppe, Heinrich C , Seldon, Ronnett , Warner, Digby F , Steenkamp, Vanessa , Kaye, Perry T
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/431778 , vital:72803 , xlink:href=" https://www.arkat-usa.org/get-file/59868/"
- Description: The screening was conducted using multi-well plates which are suited for HeLa cells in the log phase of growth with final cell density > 10 cells/cm. Each experiment normally includes a blank control, containing medium without the cells.28,45 Non-contaminated HeLa cells (6.57 x 105 cells per well) in media were allowed to grow in the incubator under an atmosphere of 5% CO2 at 37 0C for 24 h. To each well was dispensed 200 µL of HeLa culture, containing 6.57 x 105 cells under LabEAir laminar flow hood (Vivid Air, South Africa); 20 µL of resazurin dye (Sigma TOX-8) and test compound (50 µL) were added, which were then incubated in the presence of 5% CO2 at 37 0C for 24 hours in a shaker, to enhance the distribution of the dye. The absorbance of each well was measured with Bio-tek Power Wave X fluorometer (Beijing, China), and increases in fluorescence was monitored at a wavelength of 590 nm, using an excitation wavelength of 560 nm.
- Full Text:
Synthesis and dark toxicity of 5-(4-carboxyphenyl)-10, 15, 20-tris (phenyl)-porphyrinato chlorido gallium (III) when conjugated to δ-aminolevulinic acid
- Managa, Muthumuni, Mkhize, Scebi, Britton, Jonathan, Prinsloo, Earl, Nyokong, Tebello
- Authors: Managa, Muthumuni , Mkhize, Scebi , Britton, Jonathan , Prinsloo, Earl , Nyokong, Tebello
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/240014 , vital:50789 , xlink:href="https://doi.org/10.1080/00958972.2016.1223292"
- Description: 5-(4-Carboxyphenyl)-10,15,20-tris(phenyl)-porphyrinato chlorido gallium(III) (2) was synthesized and then linked to ethyl ester δ-aminolevulinic acid to form 3. There was no shift in Soret band following conjugation. The fluorescence and singlet oxygen generating behavior of the porphyrins were also investigated. The highest singlet oxygen quantum yield (ΦΔ) obtained was that of 3. Complexes 2 and 3 as well as metal free 5-(4-carboxyphenyl)-10,15,20-tris(phenyl)-porphyrinato showed no dark toxicity on MCF-7 breast cancer cells.
- Full Text:
- Authors: Managa, Muthumuni , Mkhize, Scebi , Britton, Jonathan , Prinsloo, Earl , Nyokong, Tebello
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/240014 , vital:50789 , xlink:href="https://doi.org/10.1080/00958972.2016.1223292"
- Description: 5-(4-Carboxyphenyl)-10,15,20-tris(phenyl)-porphyrinato chlorido gallium(III) (2) was synthesized and then linked to ethyl ester δ-aminolevulinic acid to form 3. There was no shift in Soret band following conjugation. The fluorescence and singlet oxygen generating behavior of the porphyrins were also investigated. The highest singlet oxygen quantum yield (ΦΔ) obtained was that of 3. Complexes 2 and 3 as well as metal free 5-(4-carboxyphenyl)-10,15,20-tris(phenyl)-porphyrinato showed no dark toxicity on MCF-7 breast cancer cells.
- Full Text:
Synthesis and optical limiting properties of new lanthanide bis-and tris-phthalocyanines
- Sekhosana, Kutloana Edward, Manyeruke, Meloddy Hlatini, Nyokong, Tebello
- Authors: Sekhosana, Kutloana Edward , Manyeruke, Meloddy Hlatini , Nyokong, Tebello
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/240063 , vital:50795 , xlink:href="https://doi.org/10.1016/j.molstruc.2016.05.068"
- Description: This manuscript reports on syntheses of new multi-decker phthalocyanines, namely: bis-{2,3,9,10,16,10,16,17,23,24-octa(4-tertbutylphenoxy) phthalocyaninato} cerium(III) (2), tris-{2,3,9,10,16,10,16,17,23,24-octa(4-tertbutylphenoxy) phthalocyaninato} dicerium(III) (4) and bis-{2,3,9,10,16,10,16,17,23,24-octa(4-tertbutylphenoxy) phthalocyaninato} gadolinium(III) (5). Complex 4 which is a tris phthalocyanine showed better nonlinear optical behavior in solution than 2 which is a bis phthalocyanine, both containing the same central metal, Ce. All the three molecules possess good optical limiting properties judged by the limiting threshold values which ranged from 0.04 to 0.09 J cm−2 with complex 5 embedded in thin films, showing the lowest value of 0.04 J cm−2. Furthermore, nonlinear optical processes responsible for reverse saturable absorption data are investigated.
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- Authors: Sekhosana, Kutloana Edward , Manyeruke, Meloddy Hlatini , Nyokong, Tebello
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/240063 , vital:50795 , xlink:href="https://doi.org/10.1016/j.molstruc.2016.05.068"
- Description: This manuscript reports on syntheses of new multi-decker phthalocyanines, namely: bis-{2,3,9,10,16,10,16,17,23,24-octa(4-tertbutylphenoxy) phthalocyaninato} cerium(III) (2), tris-{2,3,9,10,16,10,16,17,23,24-octa(4-tertbutylphenoxy) phthalocyaninato} dicerium(III) (4) and bis-{2,3,9,10,16,10,16,17,23,24-octa(4-tertbutylphenoxy) phthalocyaninato} gadolinium(III) (5). Complex 4 which is a tris phthalocyanine showed better nonlinear optical behavior in solution than 2 which is a bis phthalocyanine, both containing the same central metal, Ce. All the three molecules possess good optical limiting properties judged by the limiting threshold values which ranged from 0.04 to 0.09 J cm−2 with complex 5 embedded in thin films, showing the lowest value of 0.04 J cm−2. Furthermore, nonlinear optical processes responsible for reverse saturable absorption data are investigated.
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Synthesis and photophysicochemical properties of BODIPY dye functionalized gold nanorods for use in antimicrobial photodynamic therapy
- Kubheka, Gugu, Uddin, Imran, Amuhaya, Edith K, Mack, John, Nyokong, Tebello
- Authors: Kubheka, Gugu , Uddin, Imran , Amuhaya, Edith K , Mack, John , Nyokong, Tebello
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/239897 , vital:50778 , xlink:href="https://doi.org/10.1142/S108842461650070X"
- Description: A series of boron dipyrromethene (BODIPY) dyes with properties that are ideal for a good photosensitizer have been prepared. Functionalization with bromine atoms and attachment to gold nanoparticles through a meso-aniline group results in high singlet oxygen quantum yields and low fluorescent quantum yields. Molecular modelling was used to analyze trends in the MO energies of various brominated aniline BODIPY dyes.
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- Authors: Kubheka, Gugu , Uddin, Imran , Amuhaya, Edith K , Mack, John , Nyokong, Tebello
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/239897 , vital:50778 , xlink:href="https://doi.org/10.1142/S108842461650070X"
- Description: A series of boron dipyrromethene (BODIPY) dyes with properties that are ideal for a good photosensitizer have been prepared. Functionalization with bromine atoms and attachment to gold nanoparticles through a meso-aniline group results in high singlet oxygen quantum yields and low fluorescent quantum yields. Molecular modelling was used to analyze trends in the MO energies of various brominated aniline BODIPY dyes.
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Synthesis and physicochemical properties of zinc and indium phthalocyanines conjugated to quantum dots, gold and magnetic nanoparticles
- Osifeko, Olawale L, Nyokong, Tebello
- Authors: Osifeko, Olawale L , Nyokong, Tebello
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/188639 , vital:44771 , xlink:href="https://doi.org/10.1016/j.dyepig.2016.04.015"
- Description: This work reports on the conjugation of semiconductors quantum dots (QDs), gold (AuNPs) or Fe3O4 magnetic (MNPs) nanoparticles to 4-(4,6-diaminopyrimidin-2-ylthio) substituted indium or zinc phthalocyanines (Pcs). The QDs and MNPs were linked to the Pcs via an amide bond and by chemisorption unto AuNP surface. There is a general decrease in fluorescence quantum yields of the Pcs in the presence of all the nanoparticles. There is an increase triplet quantum yields for Pcs in the presence of AuNPs and QDs, but not in the presence of MNPs. AuNPs conjugates irrespective of the central atoms have the highest singlet oxygen quantum yield and are more photo-stable than all the other conjugates. MPcs are less photostable in the presence of MNPs.
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- Authors: Osifeko, Olawale L , Nyokong, Tebello
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/188639 , vital:44771 , xlink:href="https://doi.org/10.1016/j.dyepig.2016.04.015"
- Description: This work reports on the conjugation of semiconductors quantum dots (QDs), gold (AuNPs) or Fe3O4 magnetic (MNPs) nanoparticles to 4-(4,6-diaminopyrimidin-2-ylthio) substituted indium or zinc phthalocyanines (Pcs). The QDs and MNPs were linked to the Pcs via an amide bond and by chemisorption unto AuNP surface. There is a general decrease in fluorescence quantum yields of the Pcs in the presence of all the nanoparticles. There is an increase triplet quantum yields for Pcs in the presence of AuNPs and QDs, but not in the presence of MNPs. AuNPs conjugates irrespective of the central atoms have the highest singlet oxygen quantum yield and are more photo-stable than all the other conjugates. MPcs are less photostable in the presence of MNPs.
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Synthesis and singlet oxygen production by a phthalocyanine when embedded in asymmetric polymer membranes
- Mafukidze, Donovan M, Mashazi, Philani N, Nyokong, Tebello
- Authors: Mafukidze, Donovan M , Mashazi, Philani N , Nyokong, Tebello
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/188563 , vital:44765 , xlink:href="https://doi.org/10.1016/j.polymer.2016.10.032"
- Description: 2(3), 9(10), 16(17), 23(24)-Tetrakis-(4-aminophenoxy) phthalocyaninato indium (III) chloride (ClInTAPPc, 3) was first conjugated to two different polymers: polystyrene (PS) and polyacrylonitrile (PAN) to form 3-PS and 3-PAN. The conjugates were cast into the corresponding polymers to form membranes represented as 3-PS-membrane and 3-PAN-membrane, respectively. The prepared membranes were characterized using various techniques including scanning electron microscopy and solid state UV/Vis spectroscopy. Singlet oxygen quantum yields were higher for the 3-PS-membrane at 0.51 compared to 3-PAN-membrane at 0.35. The larger singlet oxygen also applies to 3-PS (0.63) compared to 3-PAN (0.38) when in solution.
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- Authors: Mafukidze, Donovan M , Mashazi, Philani N , Nyokong, Tebello
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/188563 , vital:44765 , xlink:href="https://doi.org/10.1016/j.polymer.2016.10.032"
- Description: 2(3), 9(10), 16(17), 23(24)-Tetrakis-(4-aminophenoxy) phthalocyaninato indium (III) chloride (ClInTAPPc, 3) was first conjugated to two different polymers: polystyrene (PS) and polyacrylonitrile (PAN) to form 3-PS and 3-PAN. The conjugates were cast into the corresponding polymers to form membranes represented as 3-PS-membrane and 3-PAN-membrane, respectively. The prepared membranes were characterized using various techniques including scanning electron microscopy and solid state UV/Vis spectroscopy. Singlet oxygen quantum yields were higher for the 3-PS-membrane at 0.51 compared to 3-PAN-membrane at 0.35. The larger singlet oxygen also applies to 3-PS (0.63) compared to 3-PAN (0.38) when in solution.
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Synthesis, characterization and electrochemistry of rhodium (iii) complexes of meso-substituted [14] tribenzotriphyrin (2.1. 1)
- Xue, Zhaoli, Wang, Yemei, Mack, John, Mkhize, Scebi, Nyokong, Tebello, Fang, Yuanyuan, Ou, Zhongping, Kadish, Karl M
- Authors: Xue, Zhaoli , Wang, Yemei , Mack, John , Mkhize, Scebi , Nyokong, Tebello , Fang, Yuanyuan , Ou, Zhongping , Kadish, Karl M
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/241043 , vital:50899 , xlink:href="https://doi.org/10.1039/C6RA03028A"
- Description: A thermal reaction using a series of [14]tribenzotriphyrins(2.1.1) (TriPs, 1a–d) with Rh2(C8H12)Cl2 provides RhIII–TriP complexes (2a–d) in 40−52% yields. The complexes were characterized by mass spectrometry, UV-visible absorption and 1H NMR spectroscopy. Single crystal X-ray analysis reveals that 2b adopts a dome-shaped conformation. The rhodium(III) ion is coordinated by the three pyrrole nitrogen atoms, two chloride ions and the nitrogen atom of an acetonitrile (CH3CN) solvent molecule. The optical spectra can be assigned using Michl's perimeter model. The L and B bands of the 2a–d complexes lie at ca. 600 and 500 nm, respectively, and are markedly red shifted relative to those of 1a–d. A reversible one-electron oxidation and two reversible one-electron reductions are observed in the cyclic voltammograms of 2a–d in CH2Cl2. The redox potentials are consistent with the optical data and the relatively narrow HOMO–LUMO gaps that are predicted in DFT calculations. TD-DFT calculations have been used to assign a third intense spectral band at 375 nm to a higher energy π → π* transition of the [14]tribenzotriphyrin(2.1.1) π-system.
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- Authors: Xue, Zhaoli , Wang, Yemei , Mack, John , Mkhize, Scebi , Nyokong, Tebello , Fang, Yuanyuan , Ou, Zhongping , Kadish, Karl M
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/241043 , vital:50899 , xlink:href="https://doi.org/10.1039/C6RA03028A"
- Description: A thermal reaction using a series of [14]tribenzotriphyrins(2.1.1) (TriPs, 1a–d) with Rh2(C8H12)Cl2 provides RhIII–TriP complexes (2a–d) in 40−52% yields. The complexes were characterized by mass spectrometry, UV-visible absorption and 1H NMR spectroscopy. Single crystal X-ray analysis reveals that 2b adopts a dome-shaped conformation. The rhodium(III) ion is coordinated by the three pyrrole nitrogen atoms, two chloride ions and the nitrogen atom of an acetonitrile (CH3CN) solvent molecule. The optical spectra can be assigned using Michl's perimeter model. The L and B bands of the 2a–d complexes lie at ca. 600 and 500 nm, respectively, and are markedly red shifted relative to those of 1a–d. A reversible one-electron oxidation and two reversible one-electron reductions are observed in the cyclic voltammograms of 2a–d in CH2Cl2. The redox potentials are consistent with the optical data and the relatively narrow HOMO–LUMO gaps that are predicted in DFT calculations. TD-DFT calculations have been used to assign a third intense spectral band at 375 nm to a higher energy π → π* transition of the [14]tribenzotriphyrin(2.1.1) π-system.
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Synthesis, Characterization, and Electronic Structures of Porphyrins Fused with Polycyclic Aromatic Ring Systems
- Okujima, Tetsuo, Mack, John, Nakamura, Jun, Kubheka, Gugu, Nyokong, Tebello, Zhu, Hua, Komobuchi, Naoki, Ono, Noboru, Yamada, Hiroko, Uno, Hidemitsu, Kobayashi, Nagao
- Authors: Okujima, Tetsuo , Mack, John , Nakamura, Jun , Kubheka, Gugu , Nyokong, Tebello , Zhu, Hua , Komobuchi, Naoki , Ono, Noboru , Yamada, Hiroko , Uno, Hidemitsu , Kobayashi, Nagao
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/240360 , vital:50827 , xlink:href="https://doi.org/10.1002/chem.201602213"
- Description: A series of porphyrins fused with acenaphthylene, phenanthroline, and benzofluoranthene polycyclic aromatic rings were prepared by means of a 3+1 porphyrin synthesis approach and subsequent retro-Diels–Alder reaction of bicyclo[2.2.2]octadiene-fused precursors. Analysis of the magnetic circular dichroism spectra and the results of time-dependent DFT calculations are used to identify the reasons for the trends observed in the wavelengths and relative intensities of the Q bands of the products. Michl's perimeter model is used as a conceptual framework to explain the changes in the relative energies of the frontier π-molecular orbitals.
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- Authors: Okujima, Tetsuo , Mack, John , Nakamura, Jun , Kubheka, Gugu , Nyokong, Tebello , Zhu, Hua , Komobuchi, Naoki , Ono, Noboru , Yamada, Hiroko , Uno, Hidemitsu , Kobayashi, Nagao
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/240360 , vital:50827 , xlink:href="https://doi.org/10.1002/chem.201602213"
- Description: A series of porphyrins fused with acenaphthylene, phenanthroline, and benzofluoranthene polycyclic aromatic rings were prepared by means of a 3+1 porphyrin synthesis approach and subsequent retro-Diels–Alder reaction of bicyclo[2.2.2]octadiene-fused precursors. Analysis of the magnetic circular dichroism spectra and the results of time-dependent DFT calculations are used to identify the reasons for the trends observed in the wavelengths and relative intensities of the Q bands of the products. Michl's perimeter model is used as a conceptual framework to explain the changes in the relative energies of the frontier π-molecular orbitals.
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Teacher code switching consistency and precision in a multilingual mathematics classroom:
- Chikiwa, Clemence, Schäfer, Marc
- Authors: Chikiwa, Clemence , Schäfer, Marc
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/141087 , vital:37943 , https://doi.org/10.1080/18117295.2016.1228823
- Description: This paper reports on a study that investigated teacher code switching consistency and precision in multilingual secondary school mathematics classrooms in South Africa. Data was obtained through interviewing and observing five lessons of each of three mathematics teachers purposively selected from three township schools in the Eastern Cape Province. Elements of Gumperz and Mercer’s work on lesson categories and Dowling’s Domains of Mathematical Practice were used to analyse data. Results showed that code switching frequency in general was inconsistent across different lessons for the same teacher.
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- Authors: Chikiwa, Clemence , Schäfer, Marc
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/141087 , vital:37943 , https://doi.org/10.1080/18117295.2016.1228823
- Description: This paper reports on a study that investigated teacher code switching consistency and precision in multilingual secondary school mathematics classrooms in South Africa. Data was obtained through interviewing and observing five lessons of each of three mathematics teachers purposively selected from three township schools in the Eastern Cape Province. Elements of Gumperz and Mercer’s work on lesson categories and Dowling’s Domains of Mathematical Practice were used to analyse data. Results showed that code switching frequency in general was inconsistent across different lessons for the same teacher.
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Teaching and Learning of ‘Water for Agriculture’in Primary Schools in Lesotho, South Africa and Zimbabwe
- Pesanayi, Tichaona V, Mashozhera, Farasten, Khitsane, Lintle
- Authors: Pesanayi, Tichaona V , Mashozhera, Farasten , Khitsane, Lintle
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/387289 , vital:68222 , xlink:href="https://www.ajol.info/index.php/sajee/article/view/152741"
- Description: Teaching youths about the subject of water for agriculture is vital in southern Africa where climate adaptation is imperative. Fresh water is a critical natural resource experiencing dangerous scarcity globally, with climate change and variability being key drivers. Agriculture consumes most of the allocated water in most of the southern African countries, so this sector needs particular water harvesting and conservation education. The Food and Agriculture Organisation (FAO) reported that 93% of cultivated land in southern Africa was rain-fed at the beginning of the 21st century. Drought hinders effective agricultural practices in poor-rainfall areas and is a common feature in most southern African countries. Increasingly frequent drought events affect Lesotho, South Africa and Zimbabwe chronically due to climate variability and change. These three countries have school curricula that carry agricultural and sustainability learning to varying extents. Agriculture is taught as a science subject, and tends to be inclined towards normative technicist approaches at the expense of traditional and innovative sustainability practices. This omission in curriculum development and teaching may miss the opportunity to learn from lessons offered by these traditional and innovative systems that have demonstrated resilience to climate variability and change. This paper explores the opportunities and enablers of sustainability learning and relevance in the primary school agriculture curricula of these three countries. The paper argues for inclusion of sustainable agricultural water learning as an act of educational quality and relevance that reflects 21st century socio-ecological, agro-climate and socioeconomic challenges in southern Africa.
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- Authors: Pesanayi, Tichaona V , Mashozhera, Farasten , Khitsane, Lintle
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/387289 , vital:68222 , xlink:href="https://www.ajol.info/index.php/sajee/article/view/152741"
- Description: Teaching youths about the subject of water for agriculture is vital in southern Africa where climate adaptation is imperative. Fresh water is a critical natural resource experiencing dangerous scarcity globally, with climate change and variability being key drivers. Agriculture consumes most of the allocated water in most of the southern African countries, so this sector needs particular water harvesting and conservation education. The Food and Agriculture Organisation (FAO) reported that 93% of cultivated land in southern Africa was rain-fed at the beginning of the 21st century. Drought hinders effective agricultural practices in poor-rainfall areas and is a common feature in most southern African countries. Increasingly frequent drought events affect Lesotho, South Africa and Zimbabwe chronically due to climate variability and change. These three countries have school curricula that carry agricultural and sustainability learning to varying extents. Agriculture is taught as a science subject, and tends to be inclined towards normative technicist approaches at the expense of traditional and innovative sustainability practices. This omission in curriculum development and teaching may miss the opportunity to learn from lessons offered by these traditional and innovative systems that have demonstrated resilience to climate variability and change. This paper explores the opportunities and enablers of sustainability learning and relevance in the primary school agriculture curricula of these three countries. The paper argues for inclusion of sustainable agricultural water learning as an act of educational quality and relevance that reflects 21st century socio-ecological, agro-climate and socioeconomic challenges in southern Africa.
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Teaching operating systems: just enough abstraction
- Authors: Machanick, Philip
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/439265 , vital:73560 , https://doi.org/10.1007/978-3-319-47680-3_10
- Description: There are two major approaches to teaching operating systems: conceptual and detailed. I explore the middle ground with an approach designed to equip students with the tools to explore detail later as the need arises, without requiring the time and grasp of detail needed to understand a full OS implementation. To meet those goals, I apply various strategies to different concepts, for example, faking the detail and using techniques from computer architecture simulation. The course aims to give students a better sense of how things work than a conceptual approach without the time required for a full implementation-based course.
- Full Text:
- Authors: Machanick, Philip
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/439265 , vital:73560 , https://doi.org/10.1007/978-3-319-47680-3_10
- Description: There are two major approaches to teaching operating systems: conceptual and detailed. I explore the middle ground with an approach designed to equip students with the tools to explore detail later as the need arises, without requiring the time and grasp of detail needed to understand a full OS implementation. To meet those goals, I apply various strategies to different concepts, for example, faking the detail and using techniques from computer architecture simulation. The course aims to give students a better sense of how things work than a conceptual approach without the time required for a full implementation-based course.
- Full Text: