A spatial perspective of the population structure and connectivity of two small tuna species, Katsuwonus pelamis (Skipjack tuna) and Euthynnus affinis (Kawakawa), in the Southwest Indian Ocean
- Authors: Taylor, Christopher John
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/467116 , vital:76816
- Description: Katsuwonus pelamis and Euthynnus affinis are two small tuna species of socio-economic and fisheries importance across the Southwest Indian Ocean (SWIO). There is currently a dearth of data and information around various aspects of their biology in the SWIO, including their population structure and connectivity. The availability of such data, including spatially represented biological data, has the potential to address current management discrepancies through the provision of more robust assessments into the stock structure and connectivity of these species and their stocks. This study explored a spatial perspective of the population structure and connectivity of K. pelamis and E. affinis fisheries in the SWIO to determine the suitability of current Indian Ocean Tuna Commission (IOTC) management measures for the species. It employed two primary data sources: a) Western Indian Ocean Marine Science Association’s (WIOMSA) Marine and Coastal Science for Management (MASMA) Project and b) IOTC. WIOMSA MASMA and IOTC data was collected throughout many countries and small island states in the SWIO, and pertained to genetic diversity, catch-weight, length-at-maturity, and morphometrics. Such data were combined and analysed to conduct geospatial analysis. Spatially illustrated genetic divergence, length-at-maturity, catch-weight, and morphometric data supported the presence of three reproductively isolated subpopulations of K. pelamis in the SWIO (Dar es Salaam, Tanzania, and Kenya; Mtwara, Tanzania, Mozambique, and Eastern Cape, South Africa; Seychelles). Only spatially depicted genetic divergence data, and morphometrics, supported that E. affinis populations were not panmictic in the SWIO, with genetic divergence data supporting the presence of three reproductively isolated subpopulations of E. affinis (Northern Mozambique and Kenya; Tanzania; Seychelles). Spatially illustrated length-at-maturity and catch-weight data however did not provide sufficient evidence supporting spatial structuring among E. affinis populations in the SWIO. Whilst the present analyses are not definitive to delineate exact stock boundaries, findings supported the contention of current IOTC management policy, indicating spatial population complexity for both species. Future research must increase representation of fishery independent samples and must investigate oceanographic variables driving potential stock separation to further support effective management reform. Until available, a precautionary approach should be adopted for the management of these species across the SWIO, considering the presently proposed distribution of their stocks separate for management. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-10-11
- Authors: Taylor, Christopher John
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/467116 , vital:76816
- Description: Katsuwonus pelamis and Euthynnus affinis are two small tuna species of socio-economic and fisheries importance across the Southwest Indian Ocean (SWIO). There is currently a dearth of data and information around various aspects of their biology in the SWIO, including their population structure and connectivity. The availability of such data, including spatially represented biological data, has the potential to address current management discrepancies through the provision of more robust assessments into the stock structure and connectivity of these species and their stocks. This study explored a spatial perspective of the population structure and connectivity of K. pelamis and E. affinis fisheries in the SWIO to determine the suitability of current Indian Ocean Tuna Commission (IOTC) management measures for the species. It employed two primary data sources: a) Western Indian Ocean Marine Science Association’s (WIOMSA) Marine and Coastal Science for Management (MASMA) Project and b) IOTC. WIOMSA MASMA and IOTC data was collected throughout many countries and small island states in the SWIO, and pertained to genetic diversity, catch-weight, length-at-maturity, and morphometrics. Such data were combined and analysed to conduct geospatial analysis. Spatially illustrated genetic divergence, length-at-maturity, catch-weight, and morphometric data supported the presence of three reproductively isolated subpopulations of K. pelamis in the SWIO (Dar es Salaam, Tanzania, and Kenya; Mtwara, Tanzania, Mozambique, and Eastern Cape, South Africa; Seychelles). Only spatially depicted genetic divergence data, and morphometrics, supported that E. affinis populations were not panmictic in the SWIO, with genetic divergence data supporting the presence of three reproductively isolated subpopulations of E. affinis (Northern Mozambique and Kenya; Tanzania; Seychelles). Spatially illustrated length-at-maturity and catch-weight data however did not provide sufficient evidence supporting spatial structuring among E. affinis populations in the SWIO. Whilst the present analyses are not definitive to delineate exact stock boundaries, findings supported the contention of current IOTC management policy, indicating spatial population complexity for both species. Future research must increase representation of fishery independent samples and must investigate oceanographic variables driving potential stock separation to further support effective management reform. Until available, a precautionary approach should be adopted for the management of these species across the SWIO, considering the presently proposed distribution of their stocks separate for management. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-10-11
Assessing the vulnerability of inland fisheries to human-induced perturbation in Lake Kariba fishery, Zambia
- Authors: Imikendu, Imbwae
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/466725 , vital:76774 , DOI https://doi.org/10.21504/10962/466725
- Description: Inland fisheries play a critical role in providing nutrition, employment, and ensuring food security among vulnerable groups in Lake Kariba. However, their value is usually undermined in developmental plans, and their contribution to a broader economic development is often undervalued. Over the past two decades, inland fisheries have stagnated. Simultaneously, there have been wide reports of increased fishing effort and declining trends in commercially valuable fish species. This has resulted into a wide socio-economic and environmental problems in various fishing communities. Lake Kariba Zambia, exemplifies this sustainability challenge, where fisheries resources and livelihoods are interlinked. A systematic peer review paper on this topic was documented, published and used for analytical work, focusing on governance and how it affects the utilization of the fisheries resources for food security and biodiversity conservation. This study had four major objectives: (i) to identify threats to fisheries’ productivity in Lake Kariba; (ii) to understand the socio-economic and environmental conditions of small-scale fisheries in Lake Kariba; (iii) to determine adaptation strategies adopted by small-scale fishers in light of the changing socio-economic and environmental conditions in Lake Kariba; (iv) to determine how management of fisheries has to change in response to the wide socio-economic and environmental challenges in the region. Using Ostrom’s framework for analysing socio-ecological systems, various socioeconomic and environmental problems faced by fishing communities in Lake Kariba were examined to devise a sustainable fisheries management plan. The study employed explorative surveys, household questionnaires, key informant interviews, focus group discussions, observations, and a review of literature to elicit secondary data. A proportional quota sampling technique was used to draw a representative sample of 300 small-scale fishers and a typology was developed using a bivariate analysis. Chi-square was used to determine fishing effort over time. Using a curve fitting estimation regression test, a trend analysis in fish production for the past thirteen years (13) years was performed. Results did not show a significant decline in fish production over the past 13 years, yet experts and the fishers reported declining trends in valuable fish species. The finding of this study provides both practical and theoretical lessons that can enhance adaptive capacity and support sustainable livelihoods in the region. A fisheries sector policy is urgently required to guide governance process and coordinate a multi-sectoral perspective to fisheries management if socio-economic progression is to be attained. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-10-11
- Authors: Imikendu, Imbwae
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/466725 , vital:76774 , DOI https://doi.org/10.21504/10962/466725
- Description: Inland fisheries play a critical role in providing nutrition, employment, and ensuring food security among vulnerable groups in Lake Kariba. However, their value is usually undermined in developmental plans, and their contribution to a broader economic development is often undervalued. Over the past two decades, inland fisheries have stagnated. Simultaneously, there have been wide reports of increased fishing effort and declining trends in commercially valuable fish species. This has resulted into a wide socio-economic and environmental problems in various fishing communities. Lake Kariba Zambia, exemplifies this sustainability challenge, where fisheries resources and livelihoods are interlinked. A systematic peer review paper on this topic was documented, published and used for analytical work, focusing on governance and how it affects the utilization of the fisheries resources for food security and biodiversity conservation. This study had four major objectives: (i) to identify threats to fisheries’ productivity in Lake Kariba; (ii) to understand the socio-economic and environmental conditions of small-scale fisheries in Lake Kariba; (iii) to determine adaptation strategies adopted by small-scale fishers in light of the changing socio-economic and environmental conditions in Lake Kariba; (iv) to determine how management of fisheries has to change in response to the wide socio-economic and environmental challenges in the region. Using Ostrom’s framework for analysing socio-ecological systems, various socioeconomic and environmental problems faced by fishing communities in Lake Kariba were examined to devise a sustainable fisheries management plan. The study employed explorative surveys, household questionnaires, key informant interviews, focus group discussions, observations, and a review of literature to elicit secondary data. A proportional quota sampling technique was used to draw a representative sample of 300 small-scale fishers and a typology was developed using a bivariate analysis. Chi-square was used to determine fishing effort over time. Using a curve fitting estimation regression test, a trend analysis in fish production for the past thirteen years (13) years was performed. Results did not show a significant decline in fish production over the past 13 years, yet experts and the fishers reported declining trends in valuable fish species. The finding of this study provides both practical and theoretical lessons that can enhance adaptive capacity and support sustainable livelihoods in the region. A fisheries sector policy is urgently required to guide governance process and coordinate a multi-sectoral perspective to fisheries management if socio-economic progression is to be attained. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-10-11
Assessment and mitigation of biosecurity risks associated with macroalgae inclusion in farmed abalone diets in South Africa
- Mwangudza, Petronilla Masika
- Authors: Mwangudza, Petronilla Masika
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/466748 , vital:76775 , DOI https://doi.org/10.21504/10962/466748
- Description: The provision of biosecure diets for use in intensive aquaculture conditions requires attention, to reduce the risk of introducing potential pathogens to the farmed stock. Such introductions could lead to infections and disease outbreaks. Despite the benefits associated with macroalgae inclusion in abalone diets, several microbial hazard cases have been reported in the animal feed and human food industries. This has necessitated the application of biosecurity measures on fresh macroalgae to reduce or eliminate potential hazards and the risks of pathogen transfer to abalone stock when used as a feed or feed supplement. The present thesis assessed the efficacy of different processing treatments, including heat, ultra-violet irradiation (UVC), different pH levels, salinity concentrations, and povo-iodine on the inactivation of potential macroalgaetransmitted abalone pathogens. The effect of the processed macroalgae on the growth, health and gut microbial composition of abalone were also explored and compared with abalone fed non-processed diets. The efficacy of the different processing treatments was initially assessed on pure cultures of the test pathogens, which included a bacterium (Vibrio anguillarum), an oomycete (Halioticida noduliformans) and the bacteriophage lambda. Data from the initial assessments were then used to select the most optimal treatments for further assessment on the test pathogens inoculated in a macroalgae matrix, to simulate a more natural scenario. The viability of the three test pathogens following exposure to different processing treatments was assessed using culture methods. The results indicated that a combination of three treatments; disinfection using povo-iodine solution (5000 mg/L) for 20 min, oven drying 40 ℃ for 8.0 h and UVC treatment for 10 min rendered all the test pathogens non-culturable. Growth trials were conducted to assess the effect of the biosecure macroalgae Ecklonia maxima, Ulva lacinulata and Gracilaria gracilis on the growth performance of the abalone Haliotis midae. The macroalgae were subjected to the combination of three biosecurity processing treatments and experimental diets were then formulated to incorporate the macroalgae that had been subjected to the biosecurity processing treatments as well as macroalgae that were not. Growth parameters of the abalone after a 150-day feeding trial were compared between the biosecure and non-bioseeure macroalgae dietary treatments. Overall, the lowest growth was observed in the abalone fed with the control diet (AbfeedTM S34R) compared to all the macroalgae diets. However, no significant differences in abalone weight and shell length were recorded between the dietary treatments after the 150-day growth trial with an overall mean final weight (} standard error) of 56.55 } 0.78 g and a mean final length of 66.26 } 0.344 mm (RM-ANOVA: F(18,63) = 0.706; p = 0.792; : F(18,63) = 0.941; p = 0.535 respectively). Similarly, the biosecurity process method (biosecure vs. non-biosecure) did not have an impact on abalone weights and shell length (p > 0.05). Moreover, specific growth rate, length gain and condition factor of abalone did not differ between the biosecure and nonbiosecure dietary treatments with overall means (}standard error) of 0.27 } 0.01 % bw/d, 1.79 } 0.07 mm/month and 1.13 } 0.01, respectively (p > 0.05). Dietary macroalgae are known to contribute to the gut microflora of abalone. To determine if the biosecure process influenced this community complex, a next generation sequencing (NGS) approach was used to identify and compare the bacterial communities in abalone that were fed diets containing macroalgae that had been subjected to biosecurity treatment and those that were not. The NGS approach was also used to determine the gut microbiome profile of the abalone fed with a formulated diet supplemented with fresh U. lacinulata and G. gracilis to assess the potential modulatory effect seaweeds and their associated microbiota may have on the gut microbiome of H. midae. The bacterial alpha diversity did not differ significantly across all the diets at family, genus and species levels (p > 0.05). No significant differences in the microbiome composition were detected indicating little or no dissimilarities of the bacterial communities between the diets for all the biosecure and non-biosecure macroalgae diets. A similar core microbiome was also observed in the digestive tracts of abalone fed with the biosecure and non-biosecure diets. It was concluded that the biosecure process did not influence the natural microbiota of abalone that were fed dietary ingredients that were subjected to the process. The findings of this research have contributed to understanding the production of biosecure macroalgae formulated diets without compromising their benefits to the growth and health of farmed abalone. The combined treatment used in this study can be applied in the macroalgae and the feed industry to produce biosecure feeds. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-10-11
- Authors: Mwangudza, Petronilla Masika
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/466748 , vital:76775 , DOI https://doi.org/10.21504/10962/466748
- Description: The provision of biosecure diets for use in intensive aquaculture conditions requires attention, to reduce the risk of introducing potential pathogens to the farmed stock. Such introductions could lead to infections and disease outbreaks. Despite the benefits associated with macroalgae inclusion in abalone diets, several microbial hazard cases have been reported in the animal feed and human food industries. This has necessitated the application of biosecurity measures on fresh macroalgae to reduce or eliminate potential hazards and the risks of pathogen transfer to abalone stock when used as a feed or feed supplement. The present thesis assessed the efficacy of different processing treatments, including heat, ultra-violet irradiation (UVC), different pH levels, salinity concentrations, and povo-iodine on the inactivation of potential macroalgaetransmitted abalone pathogens. The effect of the processed macroalgae on the growth, health and gut microbial composition of abalone were also explored and compared with abalone fed non-processed diets. The efficacy of the different processing treatments was initially assessed on pure cultures of the test pathogens, which included a bacterium (Vibrio anguillarum), an oomycete (Halioticida noduliformans) and the bacteriophage lambda. Data from the initial assessments were then used to select the most optimal treatments for further assessment on the test pathogens inoculated in a macroalgae matrix, to simulate a more natural scenario. The viability of the three test pathogens following exposure to different processing treatments was assessed using culture methods. The results indicated that a combination of three treatments; disinfection using povo-iodine solution (5000 mg/L) for 20 min, oven drying 40 ℃ for 8.0 h and UVC treatment for 10 min rendered all the test pathogens non-culturable. Growth trials were conducted to assess the effect of the biosecure macroalgae Ecklonia maxima, Ulva lacinulata and Gracilaria gracilis on the growth performance of the abalone Haliotis midae. The macroalgae were subjected to the combination of three biosecurity processing treatments and experimental diets were then formulated to incorporate the macroalgae that had been subjected to the biosecurity processing treatments as well as macroalgae that were not. Growth parameters of the abalone after a 150-day feeding trial were compared between the biosecure and non-bioseeure macroalgae dietary treatments. Overall, the lowest growth was observed in the abalone fed with the control diet (AbfeedTM S34R) compared to all the macroalgae diets. However, no significant differences in abalone weight and shell length were recorded between the dietary treatments after the 150-day growth trial with an overall mean final weight (} standard error) of 56.55 } 0.78 g and a mean final length of 66.26 } 0.344 mm (RM-ANOVA: F(18,63) = 0.706; p = 0.792; : F(18,63) = 0.941; p = 0.535 respectively). Similarly, the biosecurity process method (biosecure vs. non-biosecure) did not have an impact on abalone weights and shell length (p > 0.05). Moreover, specific growth rate, length gain and condition factor of abalone did not differ between the biosecure and nonbiosecure dietary treatments with overall means (}standard error) of 0.27 } 0.01 % bw/d, 1.79 } 0.07 mm/month and 1.13 } 0.01, respectively (p > 0.05). Dietary macroalgae are known to contribute to the gut microflora of abalone. To determine if the biosecure process influenced this community complex, a next generation sequencing (NGS) approach was used to identify and compare the bacterial communities in abalone that were fed diets containing macroalgae that had been subjected to biosecurity treatment and those that were not. The NGS approach was also used to determine the gut microbiome profile of the abalone fed with a formulated diet supplemented with fresh U. lacinulata and G. gracilis to assess the potential modulatory effect seaweeds and their associated microbiota may have on the gut microbiome of H. midae. The bacterial alpha diversity did not differ significantly across all the diets at family, genus and species levels (p > 0.05). No significant differences in the microbiome composition were detected indicating little or no dissimilarities of the bacterial communities between the diets for all the biosecure and non-biosecure macroalgae diets. A similar core microbiome was also observed in the digestive tracts of abalone fed with the biosecure and non-biosecure diets. It was concluded that the biosecure process did not influence the natural microbiota of abalone that were fed dietary ingredients that were subjected to the process. The findings of this research have contributed to understanding the production of biosecure macroalgae formulated diets without compromising their benefits to the growth and health of farmed abalone. The combined treatment used in this study can be applied in the macroalgae and the feed industry to produce biosecure feeds. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-10-11
Fishery characteristics, genetic structure, population demography and value chain of Skipjack and Kawakawa exploited in coastal waters of the Western Indian Ocean
- Authors: Mzingirwa, Fatuma Ali
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/466759 , vital:76776 , DOI https://doi.org/10.21504/10962/466759
- Description: Tuna represents a highly valuable global fishery, comprising 7.9% of the total 67.9 million metric tons (MT) of marine finfish catch. Among tuna species, Katsuwonus pelamis, skipjack and Euthynnus affinis, kawakawa are commercially important, particularly for artisanal fisheries. Skipjack is the most dominant tuna species globally, contributing over 60% to total tuna production. In the Indian Ocean (IO), skipjack catches approximately 420,000 MT annually. Kawakawa, the second most abundant neritic tuna in the IO, accounting for roughly 12% of neritic tuna landings, is primarily harvested by artisanal fleets, with annual catches of around 160,000 MT. While current assessments indicate that skipjack and kawakawa stocks in the IO are not overfished, maintaining their long-term health is crucial. This research addresses three key areas: genetic diversity, population structure, and connectivity of skipjack and kawakawa tuna in the Western Indian Ocean (WIO); size structure and reproductive characteristics of these species within the WIO; and the value chain of the Kenyan artisanal tuna fishery, focusing on skipjack and kawakawa. To achieve these objectives, skipjack and kawakawa samples were collected from Kenya, Tanzania, Mozambique, and South Africa. A non-random sampling approach was employed to obtain specimens and data from artisanal and recreational fisheries. Biological and genetic sampling were conducted concurrently. The economic value chain of the Kenyan artisanal tuna fishery was examined through questionnaires and catch data. This analysis focused on the socio-demographic profiles of key actors, the value chain structure, and associated economic benefits across four Kenyan landing sites. To investigate stock structure in WIO skipjack and kawakawa tuna, we employed tunable Genotyping-by-Sequencing (tGBS) to generate genome-wide Single Nucleotide Polymorphism (SNP) data. Skipjack analysis revealed 7005 SNPs with an average observed heterozygosity (Ho) of 0.206. While overall genetic differentiation (FST) among samples was low (global FST = 0.003) between samples (FST = 0 – 0.013), significant genetic differences were observed between skipjack samples taken north of Mtwara in southern Tanzania (i.e., northern Tanzania, Kenya and Sri Lanka) and those to the south (i.e., southern Tanzania, Mozambique and South Africa), with Seychelles falling closer to the southern grouping. Kawakawa analysis, based on 14806 SNPs and an average Ho of 0.2585, indicated a patchy distribution of low but significant genetic differentiation among WIO populations (global FST = 0.018) between-sample (FST = 0.003 – 0.036) but with no obvious geographically-based pattern. However, unlike skipjack, a clear geographic pattern in genetic structure was not evident for kawakawa. Skipjack and kawakawa populations in the WIO exhibited seasonal fluctuations in size distribution, potentially influenced by environmental conditions and fishing practices. Landings of both species were male-biased, with sex ratios of 58% and 53% for skipjack and kawakawa, respectively. Female skipjack reached sexual maturity at a fork length (FL) of 42.0 cm, while males matured at 47.0 cm FL. For kawakawa, female and male maturation lengths were 44.0 cm FL and 45.3 cm FL, respectively. Spawning occurred throughout the year, with peak activity coinciding with the Northeast Monsoon (NEM) season. Our analysis of the artisanal tuna value chain indicates that fishers primarily sell their catch to agents (53%), with the remaining proportions going to traders (20%) and processors (18%). Processors, predominantly women, play a key role in the value chain and realized the highest net profit margin (49.5%). Limited post-harvest infrastructure, inadequate transportation, and poor marketing conditions were identified as key challenges impacting the quality of fish lowering their income. These challenges disproportionately affect fishers with limited access to market information and financial resources. The findings demonstrate the need for multi-level interventions to optimize benefits from the artisanal tuna fishery along the entire value chain taking into consideration the economic, environmental, and social dimensions. This research provides crucial information for effective tuna management in the IO. Current management practices treat skipjack and kawakawa as a single, homogenous population across the entire IO. However, our genetic findings suggest the presence of distinct population groups (stocks) for both species within the WIO. Moreover, seasonal variations in size structure and reproductive characteristics observed support this hypothesis of multiple stocks. These results emphasize the need for a precautionary approach to tuna management in the region. Collaborative efforts among countries are essential to develop sustainable fisheries management strategies that consider biological, economic, and social factors. By integrating these perspectives, we can ensure the long-term health of tuna populations while supporting the livelihoods of fishing communities. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-10-11
- Authors: Mzingirwa, Fatuma Ali
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/466759 , vital:76776 , DOI https://doi.org/10.21504/10962/466759
- Description: Tuna represents a highly valuable global fishery, comprising 7.9% of the total 67.9 million metric tons (MT) of marine finfish catch. Among tuna species, Katsuwonus pelamis, skipjack and Euthynnus affinis, kawakawa are commercially important, particularly for artisanal fisheries. Skipjack is the most dominant tuna species globally, contributing over 60% to total tuna production. In the Indian Ocean (IO), skipjack catches approximately 420,000 MT annually. Kawakawa, the second most abundant neritic tuna in the IO, accounting for roughly 12% of neritic tuna landings, is primarily harvested by artisanal fleets, with annual catches of around 160,000 MT. While current assessments indicate that skipjack and kawakawa stocks in the IO are not overfished, maintaining their long-term health is crucial. This research addresses three key areas: genetic diversity, population structure, and connectivity of skipjack and kawakawa tuna in the Western Indian Ocean (WIO); size structure and reproductive characteristics of these species within the WIO; and the value chain of the Kenyan artisanal tuna fishery, focusing on skipjack and kawakawa. To achieve these objectives, skipjack and kawakawa samples were collected from Kenya, Tanzania, Mozambique, and South Africa. A non-random sampling approach was employed to obtain specimens and data from artisanal and recreational fisheries. Biological and genetic sampling were conducted concurrently. The economic value chain of the Kenyan artisanal tuna fishery was examined through questionnaires and catch data. This analysis focused on the socio-demographic profiles of key actors, the value chain structure, and associated economic benefits across four Kenyan landing sites. To investigate stock structure in WIO skipjack and kawakawa tuna, we employed tunable Genotyping-by-Sequencing (tGBS) to generate genome-wide Single Nucleotide Polymorphism (SNP) data. Skipjack analysis revealed 7005 SNPs with an average observed heterozygosity (Ho) of 0.206. While overall genetic differentiation (FST) among samples was low (global FST = 0.003) between samples (FST = 0 – 0.013), significant genetic differences were observed between skipjack samples taken north of Mtwara in southern Tanzania (i.e., northern Tanzania, Kenya and Sri Lanka) and those to the south (i.e., southern Tanzania, Mozambique and South Africa), with Seychelles falling closer to the southern grouping. Kawakawa analysis, based on 14806 SNPs and an average Ho of 0.2585, indicated a patchy distribution of low but significant genetic differentiation among WIO populations (global FST = 0.018) between-sample (FST = 0.003 – 0.036) but with no obvious geographically-based pattern. However, unlike skipjack, a clear geographic pattern in genetic structure was not evident for kawakawa. Skipjack and kawakawa populations in the WIO exhibited seasonal fluctuations in size distribution, potentially influenced by environmental conditions and fishing practices. Landings of both species were male-biased, with sex ratios of 58% and 53% for skipjack and kawakawa, respectively. Female skipjack reached sexual maturity at a fork length (FL) of 42.0 cm, while males matured at 47.0 cm FL. For kawakawa, female and male maturation lengths were 44.0 cm FL and 45.3 cm FL, respectively. Spawning occurred throughout the year, with peak activity coinciding with the Northeast Monsoon (NEM) season. Our analysis of the artisanal tuna value chain indicates that fishers primarily sell their catch to agents (53%), with the remaining proportions going to traders (20%) and processors (18%). Processors, predominantly women, play a key role in the value chain and realized the highest net profit margin (49.5%). Limited post-harvest infrastructure, inadequate transportation, and poor marketing conditions were identified as key challenges impacting the quality of fish lowering their income. These challenges disproportionately affect fishers with limited access to market information and financial resources. The findings demonstrate the need for multi-level interventions to optimize benefits from the artisanal tuna fishery along the entire value chain taking into consideration the economic, environmental, and social dimensions. This research provides crucial information for effective tuna management in the IO. Current management practices treat skipjack and kawakawa as a single, homogenous population across the entire IO. However, our genetic findings suggest the presence of distinct population groups (stocks) for both species within the WIO. Moreover, seasonal variations in size structure and reproductive characteristics observed support this hypothesis of multiple stocks. These results emphasize the need for a precautionary approach to tuna management in the region. Collaborative efforts among countries are essential to develop sustainable fisheries management strategies that consider biological, economic, and social factors. By integrating these perspectives, we can ensure the long-term health of tuna populations while supporting the livelihoods of fishing communities. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-10-11
Optimising Integrated Multitrophic Aquaculture (IMTA) on a South African abalone farm
- Authors: Falade, Abiodun Emmanuel
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/466712 , vital:76772 , DOI https://doi.org/10.21504/10962/466712
- Description: The efficiency of fed nutrient utilisation in integrated multitrophic aquaculture (IMTA) system was evaluated on a South African abalone farm. On many commercial abalone farms in South Africa, Haliotis midae are fed a combination of pelleted feed and live macroalgae cultured downstream in abalone effluent. This production technique reduces the discharge of dissolved nutrients into the environment and improves farm productivity as unquantified proportion of the waste metabolites is captured as macroalgal biomass. However, the solid waste from abalone culture tanks remains unutilised and discharged to the coastal environments. Thus, there is scope to improve the dissolved nutrients removal efficiency of the macroalgae and to reduce the discharge of particulate nutrients using detritus waste extractive organisms. The present study aimed at the production and environmental performance of a shore-based abalone/macroalgae IMTA improving farm in South Africa by improving the nutrient utilisation efficiency of farmed abalone and seaweed and testing the waste solids removal potential of a sea cucumber species. Monoculture systems, where abalone and seaweed were cultured separately, both in fresh seawater (salinity: 35 g/L), were compared with an integrated culture system where the seaweed (Ulva lacinulata) was cultured downstream in the wastewater flowing from abalone tanks that were up-channel. Based on the findings from quantifying the performance of these production systems, methods to improve the nutrient utilisation and production efficiencies of the production systems were explored. These assessed methods included (1) the removal of abalone biodeposits by detritus extractive sea cucumber production, (2) evaluating the potential of farmed macroalgae as supplement in formulated diet, and (3) replacing mineral fertilisers with eco-friendly live microbial fertilisers for seaweed farming. Furthermore, the overall environmental performance of the two farm systems was quantified using a life cycle analysis methodology. Monitoring of the nutrient flows through the monoculture and IMTA systems revealed that the highest inputs of nutrients (nitrogen and phosphorus) into the abalone and seaweed culture tanks of the two production systems were abalone feed pellet (70-81%) and mineral fertilisers respectively (63-93%). About 48-51% of the nitrogen supplied from the feed was utilised by abalone in the IMTA and monoculture systems, while the remaining portion was lost as organic waste nitrogen on the production tank floor (20-30%) and as dissolved nitrogen in post-abalone tank effluent (30-36%). In the seaweed tanks receiving abalone effluent (IMTA), 69% of the dissolved nitrogen input was absorbed by cultured Ulva while 25% of the nitrogen was lost to the post-seaweed effluent which returned to the environment. However, in the monoculture system, 52% of the nitrogen from supplemented inorganic fertiliser was absorbed by cultured Ulva while ca. 46% of the nutrient was lost to coastal waters through the post-seaweed effluent. Moreover, while the feed accounted for ca. 74-78% input of the phosphorus in abalone of the two production systems, not more than 19% and 13% of this phosphorus was utilised by H. midae in the IMTA and monoculture systems respectively, while the largest portions were lost as organic waste in the sediment (34-45%) and dissolved waste phosphorus in the effluent (33-54%). In the seaweed tanks, a small portion (11-15%) of supplied phosphorus was removed by farmed Ulva while 77-89% was lost in the post-seaweed effluent discharged to coastal environment. The substitution of 50% mineral fertilisers with live microbial fertilisers during seaweed production significantly reduced the discharge of dissolved nitrogen and phosphorus from macroalgae raceways to coastal environment by 55 and 45% respectively, without impacting their growth, yield and nutrient compositions. A life cycle analysis of the measured energy and nutrients utilisation efficiency of these production systems was compiled, and the impacts of the inputs and outputs from each production system on the environment was assessed. The electrical energy input to abalone and seaweed tanks constituted the highest contribution to all assessed environmental impact categories for the two production systems, followed by the contributions from the nutrients supplied to farmed abalone (formulated diet) and seaweed (mineral fertilisers). The impact of these inputs on the environment was most evident on marine aquatic ecotoxicity being 2.11E+03 kg 1.4-DB eq and 4.43E+03 kg 1.4-DB eq for IMTA and monoculture systems respectively. The measured impact of seaweed aquaculture on the environment was reduced by 50-52% when Ulva was cultured in abalone effluent (IMTA) compared to culture in fresh seawater (monoculture). However, the input of chemical fertilisers in the two systems of cultivation resulted in similar eutrophication potentials (8.09 - 8.41E-02 kg PO4--- eq). To reduce the solid waste discharge from abalone tanks, and create an additional high-value crop, an endemic sea cucumber species (Neostichopus grammatus) was introduced on the floor of the abalone culture tanks in a pilot abalone/detritivore/macroalgae IMTA system. The sea cucumber utilised the biodeposits in abalone tanks as food which reduced organic solid discharge to the environment by 11%. However, the sea cucumbers displayed poor nutrient utilisation, a negative growth rate (- 0.59% day-1) and 49% weight loss by the end of the trial that was probably due to sub-optimal habitat conditions (lack of a sand substrate). The potential of including farmed Ulva (IMTA and monoculture) meal in pelleted feed for H. midae was evaluated as a means of improving farming efficiency and reducing the levels of fishmeal and soya in the pellet. In an initial trial, Ulva was included at 12% dry weight in commercial diet and fed to farmed H. midae for 244 d. The 12% inclusion of IMTA and monoculture Ulva resulted in poor feed conversion and nutrient utilisation by H. midae. In a follow-up trial which tested graded inclusion levels of Ulva meal (0.75, 1.50, 3.00, 6.00 and 12.00%), the growth rate and feed utilisation of H. midae was enhanced at a 0.75-6.00% inclusion level of the seaweed in the diet, while at a 6.00-12.00% inclusion level growth rates and feed conversion efficiencies decreased. Therefore, it is recommended that for sub-adult South African abalone, up to 6.00% IMTA Ulva meal can be included in the diet formulation without impacting their growth performance and nutrient utilisation efficiency negatively. This present study contributes to the understanding of the nutrient utilisation dynamics on integrated abalone farms in South Africa. The evidence from the different trials suggests the IMTA techniques tested could be adopted to improve the production performance and reduce the impact of abalone farming on the environment. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-10-11
- Authors: Falade, Abiodun Emmanuel
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/466712 , vital:76772 , DOI https://doi.org/10.21504/10962/466712
- Description: The efficiency of fed nutrient utilisation in integrated multitrophic aquaculture (IMTA) system was evaluated on a South African abalone farm. On many commercial abalone farms in South Africa, Haliotis midae are fed a combination of pelleted feed and live macroalgae cultured downstream in abalone effluent. This production technique reduces the discharge of dissolved nutrients into the environment and improves farm productivity as unquantified proportion of the waste metabolites is captured as macroalgal biomass. However, the solid waste from abalone culture tanks remains unutilised and discharged to the coastal environments. Thus, there is scope to improve the dissolved nutrients removal efficiency of the macroalgae and to reduce the discharge of particulate nutrients using detritus waste extractive organisms. The present study aimed at the production and environmental performance of a shore-based abalone/macroalgae IMTA improving farm in South Africa by improving the nutrient utilisation efficiency of farmed abalone and seaweed and testing the waste solids removal potential of a sea cucumber species. Monoculture systems, where abalone and seaweed were cultured separately, both in fresh seawater (salinity: 35 g/L), were compared with an integrated culture system where the seaweed (Ulva lacinulata) was cultured downstream in the wastewater flowing from abalone tanks that were up-channel. Based on the findings from quantifying the performance of these production systems, methods to improve the nutrient utilisation and production efficiencies of the production systems were explored. These assessed methods included (1) the removal of abalone biodeposits by detritus extractive sea cucumber production, (2) evaluating the potential of farmed macroalgae as supplement in formulated diet, and (3) replacing mineral fertilisers with eco-friendly live microbial fertilisers for seaweed farming. Furthermore, the overall environmental performance of the two farm systems was quantified using a life cycle analysis methodology. Monitoring of the nutrient flows through the monoculture and IMTA systems revealed that the highest inputs of nutrients (nitrogen and phosphorus) into the abalone and seaweed culture tanks of the two production systems were abalone feed pellet (70-81%) and mineral fertilisers respectively (63-93%). About 48-51% of the nitrogen supplied from the feed was utilised by abalone in the IMTA and monoculture systems, while the remaining portion was lost as organic waste nitrogen on the production tank floor (20-30%) and as dissolved nitrogen in post-abalone tank effluent (30-36%). In the seaweed tanks receiving abalone effluent (IMTA), 69% of the dissolved nitrogen input was absorbed by cultured Ulva while 25% of the nitrogen was lost to the post-seaweed effluent which returned to the environment. However, in the monoculture system, 52% of the nitrogen from supplemented inorganic fertiliser was absorbed by cultured Ulva while ca. 46% of the nutrient was lost to coastal waters through the post-seaweed effluent. Moreover, while the feed accounted for ca. 74-78% input of the phosphorus in abalone of the two production systems, not more than 19% and 13% of this phosphorus was utilised by H. midae in the IMTA and monoculture systems respectively, while the largest portions were lost as organic waste in the sediment (34-45%) and dissolved waste phosphorus in the effluent (33-54%). In the seaweed tanks, a small portion (11-15%) of supplied phosphorus was removed by farmed Ulva while 77-89% was lost in the post-seaweed effluent discharged to coastal environment. The substitution of 50% mineral fertilisers with live microbial fertilisers during seaweed production significantly reduced the discharge of dissolved nitrogen and phosphorus from macroalgae raceways to coastal environment by 55 and 45% respectively, without impacting their growth, yield and nutrient compositions. A life cycle analysis of the measured energy and nutrients utilisation efficiency of these production systems was compiled, and the impacts of the inputs and outputs from each production system on the environment was assessed. The electrical energy input to abalone and seaweed tanks constituted the highest contribution to all assessed environmental impact categories for the two production systems, followed by the contributions from the nutrients supplied to farmed abalone (formulated diet) and seaweed (mineral fertilisers). The impact of these inputs on the environment was most evident on marine aquatic ecotoxicity being 2.11E+03 kg 1.4-DB eq and 4.43E+03 kg 1.4-DB eq for IMTA and monoculture systems respectively. The measured impact of seaweed aquaculture on the environment was reduced by 50-52% when Ulva was cultured in abalone effluent (IMTA) compared to culture in fresh seawater (monoculture). However, the input of chemical fertilisers in the two systems of cultivation resulted in similar eutrophication potentials (8.09 - 8.41E-02 kg PO4--- eq). To reduce the solid waste discharge from abalone tanks, and create an additional high-value crop, an endemic sea cucumber species (Neostichopus grammatus) was introduced on the floor of the abalone culture tanks in a pilot abalone/detritivore/macroalgae IMTA system. The sea cucumber utilised the biodeposits in abalone tanks as food which reduced organic solid discharge to the environment by 11%. However, the sea cucumbers displayed poor nutrient utilisation, a negative growth rate (- 0.59% day-1) and 49% weight loss by the end of the trial that was probably due to sub-optimal habitat conditions (lack of a sand substrate). The potential of including farmed Ulva (IMTA and monoculture) meal in pelleted feed for H. midae was evaluated as a means of improving farming efficiency and reducing the levels of fishmeal and soya in the pellet. In an initial trial, Ulva was included at 12% dry weight in commercial diet and fed to farmed H. midae for 244 d. The 12% inclusion of IMTA and monoculture Ulva resulted in poor feed conversion and nutrient utilisation by H. midae. In a follow-up trial which tested graded inclusion levels of Ulva meal (0.75, 1.50, 3.00, 6.00 and 12.00%), the growth rate and feed utilisation of H. midae was enhanced at a 0.75-6.00% inclusion level of the seaweed in the diet, while at a 6.00-12.00% inclusion level growth rates and feed conversion efficiencies decreased. Therefore, it is recommended that for sub-adult South African abalone, up to 6.00% IMTA Ulva meal can be included in the diet formulation without impacting their growth performance and nutrient utilisation efficiency negatively. This present study contributes to the understanding of the nutrient utilisation dynamics on integrated abalone farms in South Africa. The evidence from the different trials suggests the IMTA techniques tested could be adopted to improve the production performance and reduce the impact of abalone farming on the environment. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-10-11
The reproductive biology of warty sea cucumber, Neostichopus grammatus Clark (Holothuroidea: Echinodermata) under natural and integrated multi-trophic aquaculture (IMTA) conditions in the Eastern Cape Province, South Africa
- Authors: Senekal, Heleen Ester
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/464451 , vital:76512
- Description: This study investigated the reproductive cycle of the warty sea cucumber, Neostichopus grammatus, in the wild and in a farming environment, to test its suitability for co-culture with abalone Haliotis midae in an IMTA system. The research formed part of a broader EU H2020 program, Aquavitae, and was conducted at Wild Coast Abalone in South Africa. The study investigated several environmental parameters, such as temperature, day length, and substrate, to determine if these have any effects on the gonadal development and reproductive cycle. The natural gametogenic cycle of wild warty sea cucumbers was determined by monitoring the Gonadosomatic Index (GI) values monthly for a period of 16 months. The GI index was validated by means of other maturity assessment techniques including measurement of the size and colour of the gonads, oocyte diameter measurements and gonad histology sections. Wild collected sea cucumbers were maintained in abalone farm rearing tanks on a diet of abalone feed and faecal waste for a period of 10 months, with GI values being determined monthly. The wild and farmed sea cucumber GI data was correlated with environmental parameters. Seasonal water temperature was strongly correlated with the GI values of wild warty sea cucumbers, suggesting that water temperature likely influences gonadal development. Sea cucumbers matured sexually during colder months (May to September) and spawned from September to February, during summer. The results of oocyte measurements, macroscopic and microscopic analyses, and histological analyses in combination with the GI values, provides a practical indicator of sexual maturity for captive breeding purposes. Farmed warty sea cucumbers followed the same annual reproductive cycle as wild sea cucumbers, however they came into spawning condition approximately one month later than wild conspecifics. As the farm water temperature was significantly higher than the ambient ocean water temperature, it was hypothesised that this may have affected the gonadal development of the farmed sea cucumbers. Furthermore, the farm-reared sea cucumbers lost weight and condition, indicating a nutritional deficiency. Previous studies indicated that the presence of a sand sediment facilitated the assimilation of organic detrital matter by sea cucumbers. An additional trial was thus set up to determine the effect of a sand sediment and cooler ambient temperature on the growth and gonadal development of the warty sea cucumbers. The growth and GI values of sea cucumbers in the cool water treatment did not differ significantly from the ambient temperature control groups. However, the addition of a sand substrate to the sea cucumber tanks had a significant positive growth effect on sea cucumber body mass and GI values. Sea cucumbers fed a diet of abalone waste material on the sand substrate exhibited final average GI values of 2.99 % (SE ±0.56). In contrast, sea cucumbers kept in bare tanks and only received abalone waste as food had significantly lower average GI values of 1.36 % (SE±0.2). This suggests that the provision of a sand sustrate in sea cucumber rearing containers is essential for the adequate nutrition and gonadal development. The results of this study indicate that the warty sea cucumber is a promising candidate for inlcusion in an IMTA system with South African perlemoen abalone. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-10-11
- Authors: Senekal, Heleen Ester
- Date: 2024-10-11
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/464451 , vital:76512
- Description: This study investigated the reproductive cycle of the warty sea cucumber, Neostichopus grammatus, in the wild and in a farming environment, to test its suitability for co-culture with abalone Haliotis midae in an IMTA system. The research formed part of a broader EU H2020 program, Aquavitae, and was conducted at Wild Coast Abalone in South Africa. The study investigated several environmental parameters, such as temperature, day length, and substrate, to determine if these have any effects on the gonadal development and reproductive cycle. The natural gametogenic cycle of wild warty sea cucumbers was determined by monitoring the Gonadosomatic Index (GI) values monthly for a period of 16 months. The GI index was validated by means of other maturity assessment techniques including measurement of the size and colour of the gonads, oocyte diameter measurements and gonad histology sections. Wild collected sea cucumbers were maintained in abalone farm rearing tanks on a diet of abalone feed and faecal waste for a period of 10 months, with GI values being determined monthly. The wild and farmed sea cucumber GI data was correlated with environmental parameters. Seasonal water temperature was strongly correlated with the GI values of wild warty sea cucumbers, suggesting that water temperature likely influences gonadal development. Sea cucumbers matured sexually during colder months (May to September) and spawned from September to February, during summer. The results of oocyte measurements, macroscopic and microscopic analyses, and histological analyses in combination with the GI values, provides a practical indicator of sexual maturity for captive breeding purposes. Farmed warty sea cucumbers followed the same annual reproductive cycle as wild sea cucumbers, however they came into spawning condition approximately one month later than wild conspecifics. As the farm water temperature was significantly higher than the ambient ocean water temperature, it was hypothesised that this may have affected the gonadal development of the farmed sea cucumbers. Furthermore, the farm-reared sea cucumbers lost weight and condition, indicating a nutritional deficiency. Previous studies indicated that the presence of a sand sediment facilitated the assimilation of organic detrital matter by sea cucumbers. An additional trial was thus set up to determine the effect of a sand sediment and cooler ambient temperature on the growth and gonadal development of the warty sea cucumbers. The growth and GI values of sea cucumbers in the cool water treatment did not differ significantly from the ambient temperature control groups. However, the addition of a sand substrate to the sea cucumber tanks had a significant positive growth effect on sea cucumber body mass and GI values. Sea cucumbers fed a diet of abalone waste material on the sand substrate exhibited final average GI values of 2.99 % (SE ±0.56). In contrast, sea cucumbers kept in bare tanks and only received abalone waste as food had significantly lower average GI values of 1.36 % (SE±0.2). This suggests that the provision of a sand sustrate in sea cucumber rearing containers is essential for the adequate nutrition and gonadal development. The results of this study indicate that the warty sea cucumber is a promising candidate for inlcusion in an IMTA system with South African perlemoen abalone. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-10-11
The use of aeration in constructed wetlands and the potential for earthworm and crop production
- Authors: Cesler, Lia
- Date: 2024-10-11
- Subjects: Constructed wetlands South Africa , Breweries South Africa , Water Purification , Land treatment of wastewater , Sewage Purification Aeration
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/464440 , vital:76511
- Description: Constructed wetlands (CWs) are used in a variety of industries to treat effluent for safe reuse or discharge. They can however take up large areas of land. Adding oxygen can increase treatment efficiency, as it is vital to many nutrient removal pathways, such as nitrification and aerobic respiration. Increasing efficiency can decrease the cost of treatment and reduce land area needed to operate a CW. The study aimed to find the best method of aerating wetlands treating brewery effluent, the potential production of worms and plants in the system and their effects on water treatment. The first experiment tested a hybrid constructed wetland, using small (1.0 m3) ebb and flow filtration additions to a horizontal subsurface flow constructed wetland against nanobubble aeration in a similar horizontal flow wetland cell. It was not possible to add replicates to the study, but findings prompted further investigation into the hybrid setup. Experiment two focussed on the ebb and flow filters within the hybrid system comparing the presence of plants and worms. The ebb and flow filters increased dissolved oxygen (DO) from an average inflow of 3.89 mg/L to an outflow of 5.70 mg/L and decreased the ammonia content of the effluent from 14.8 mg/L to 11.2 mg/L. Swiss chard (Beta vulgaris) was successfully grown in the system and was found not to affect DO. The addition of both Swiss chard and earthworms (Eisenia fetida) was found to decrease PO43- by 17.69 ± 1.36 %. Experiment 3 compared the previous ebb and flow filters to trickle filters with the addition of celery (Apium graveolens) to both systems to determine which filter treated the effluent more effectively. The ebb and flow filters performed better than the trickle filters, increasing the DO by 37.54 ± 0.06 % compared to 19.22 ± 0.06 %. Celery increased this change in both systems, to 44.16 ± 0.06 % in the ebb and flow filters and 30.96 ± 0.06 % in the trickle filters. The ebb and flow filters were also able to decrease the NH3-N concentration by 36.1 ± 0.2 %. This effluent could sustain plant growth; Swiss chard grew at a rate of 0.04 ± 0.02 g/g/d and celery at 0.2 ± 0.1 g/g/d. Ebb and flow filters are better at aerating brewery effluent and decreasing nutrient load than nanobubble pumps and trickle filters. The addition of ebb and flow filters to the CW treating brewery effluent has the potential to improve nutrient removal and provide an edible crop. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-10-11
- Authors: Cesler, Lia
- Date: 2024-10-11
- Subjects: Constructed wetlands South Africa , Breweries South Africa , Water Purification , Land treatment of wastewater , Sewage Purification Aeration
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/464440 , vital:76511
- Description: Constructed wetlands (CWs) are used in a variety of industries to treat effluent for safe reuse or discharge. They can however take up large areas of land. Adding oxygen can increase treatment efficiency, as it is vital to many nutrient removal pathways, such as nitrification and aerobic respiration. Increasing efficiency can decrease the cost of treatment and reduce land area needed to operate a CW. The study aimed to find the best method of aerating wetlands treating brewery effluent, the potential production of worms and plants in the system and their effects on water treatment. The first experiment tested a hybrid constructed wetland, using small (1.0 m3) ebb and flow filtration additions to a horizontal subsurface flow constructed wetland against nanobubble aeration in a similar horizontal flow wetland cell. It was not possible to add replicates to the study, but findings prompted further investigation into the hybrid setup. Experiment two focussed on the ebb and flow filters within the hybrid system comparing the presence of plants and worms. The ebb and flow filters increased dissolved oxygen (DO) from an average inflow of 3.89 mg/L to an outflow of 5.70 mg/L and decreased the ammonia content of the effluent from 14.8 mg/L to 11.2 mg/L. Swiss chard (Beta vulgaris) was successfully grown in the system and was found not to affect DO. The addition of both Swiss chard and earthworms (Eisenia fetida) was found to decrease PO43- by 17.69 ± 1.36 %. Experiment 3 compared the previous ebb and flow filters to trickle filters with the addition of celery (Apium graveolens) to both systems to determine which filter treated the effluent more effectively. The ebb and flow filters performed better than the trickle filters, increasing the DO by 37.54 ± 0.06 % compared to 19.22 ± 0.06 %. Celery increased this change in both systems, to 44.16 ± 0.06 % in the ebb and flow filters and 30.96 ± 0.06 % in the trickle filters. The ebb and flow filters were also able to decrease the NH3-N concentration by 36.1 ± 0.2 %. This effluent could sustain plant growth; Swiss chard grew at a rate of 0.04 ± 0.02 g/g/d and celery at 0.2 ± 0.1 g/g/d. Ebb and flow filters are better at aerating brewery effluent and decreasing nutrient load than nanobubble pumps and trickle filters. The addition of ebb and flow filters to the CW treating brewery effluent has the potential to improve nutrient removal and provide an edible crop. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-10-11
A revised checklist of extant freshwater fishes of the Zambezian Lowveld Ecoregion, with taxonomic revision of the lowveld largescale yellowfish, Labeobarbus marequensis
- Authors: Ntokoane, Tholoana
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435943 , vital:73214
- Description: Access restricted. Expected release in 2025. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-04-05
- Authors: Ntokoane, Tholoana
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435943 , vital:73214
- Description: Access restricted. Expected release in 2025. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-04-05
Co-occurrence of Sympatric Poroderma Species
- Authors: Watson, Ralph Gareth Andrew
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/436649 , vital:73289 , DOI 10.21504/10962/436649
- Description: Species exploiting similar ecological niches are expected to adapt their behaviour, which can either promote or hinder coexistence. This study examined the ecological (dis)similarity between the endemic Poroderma africanum (pyjama catshark) and Poroderma pantherinum (leopard catshark) in Mossel Bay, South Africa. The co-occurrence of these species was examined along the ecological axes of time, trophic position and space, between October 2015 and April 2018, through the use of Baited Remote Underwater Videos (BRUV), gastric lavage, and acoustic telemetry. Through the deployment of 197 BRUVs, P. africanum showed a seasonal, higher Relative Abundance (RA = 0.52), occurring more frequently during winter months, while showing an overall decline in RA over the course of the study period. Poroderma pantherinum on the other hand, showed a lower, unseasonal RA (0.20), remaining relatively stable throughout the study period. The BRUV deployments indicated that the two species showed a positive co-occurrence with one other, being sighted in BRUV deployments more frequently together as opposed to in isolation/at random. Acoustic telemetry indicated that the two species were active during different diel periods, influenced by a complex combination of tidal and diel rhythms, while P. pantherinum showed a higher residency compared to P. africanum (P. pantherinum: Continuous Residency Time (CRT)24 = 3.32 days (mean), 95% CI: 2.53–4.11 days; P. africanum: CRT24 = 2.01 days, 95% CI: 1.66–2.36 days). While the two species are sympatric in nature, and have an overlapping, endemic, distribution, acoustic telemetry indicated that 1 ABSTRACT P. africanum showed higher degrees of movement throughout the acoustic receiver array (P. africanum: edge density (ED) = 0.25; P. pantherinum: ED = 0.12); however, certain areas of the bay showed to be of high importance for both species. Both species revealed high levels of intra- and inter-specific variation in both residency and movement behaviour. Gastric lavage indicated partially overlapping trophic niches, between the two Poroderma spp. Poroderma africanum had a generalist diet, dominated by teleosts (Index of Relative Importance (IRI)% = 22.69), octopus (IRI% = 11.48) and discarded bait (IRI% = 64.54), while P. pantherinum showed indications of being a specialist predator, with a diet dominated by cephalopods (IRI% = 83.68). The two Poroderma spp. showed a partially overlapping, but separate trophic niches, while displaying spatial dissimilarity in diet. The study suggests that the two species are able to coexist within the same geographical area through niche differentiation across trophic and temporal ecological axes, with varying spatial use. The intra- and inter-specific differences between the two species may complicate elasmobranch management efforts for these co-occurring endemic catsharks, and as such, efforts should follow either an individual species approach, which is often not feasible, or an ecosystem-based approach, as opposed to considering the genus as a whole. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-04-05
- Authors: Watson, Ralph Gareth Andrew
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/436649 , vital:73289 , DOI 10.21504/10962/436649
- Description: Species exploiting similar ecological niches are expected to adapt their behaviour, which can either promote or hinder coexistence. This study examined the ecological (dis)similarity between the endemic Poroderma africanum (pyjama catshark) and Poroderma pantherinum (leopard catshark) in Mossel Bay, South Africa. The co-occurrence of these species was examined along the ecological axes of time, trophic position and space, between October 2015 and April 2018, through the use of Baited Remote Underwater Videos (BRUV), gastric lavage, and acoustic telemetry. Through the deployment of 197 BRUVs, P. africanum showed a seasonal, higher Relative Abundance (RA = 0.52), occurring more frequently during winter months, while showing an overall decline in RA over the course of the study period. Poroderma pantherinum on the other hand, showed a lower, unseasonal RA (0.20), remaining relatively stable throughout the study period. The BRUV deployments indicated that the two species showed a positive co-occurrence with one other, being sighted in BRUV deployments more frequently together as opposed to in isolation/at random. Acoustic telemetry indicated that the two species were active during different diel periods, influenced by a complex combination of tidal and diel rhythms, while P. pantherinum showed a higher residency compared to P. africanum (P. pantherinum: Continuous Residency Time (CRT)24 = 3.32 days (mean), 95% CI: 2.53–4.11 days; P. africanum: CRT24 = 2.01 days, 95% CI: 1.66–2.36 days). While the two species are sympatric in nature, and have an overlapping, endemic, distribution, acoustic telemetry indicated that 1 ABSTRACT P. africanum showed higher degrees of movement throughout the acoustic receiver array (P. africanum: edge density (ED) = 0.25; P. pantherinum: ED = 0.12); however, certain areas of the bay showed to be of high importance for both species. Both species revealed high levels of intra- and inter-specific variation in both residency and movement behaviour. Gastric lavage indicated partially overlapping trophic niches, between the two Poroderma spp. Poroderma africanum had a generalist diet, dominated by teleosts (Index of Relative Importance (IRI)% = 22.69), octopus (IRI% = 11.48) and discarded bait (IRI% = 64.54), while P. pantherinum showed indications of being a specialist predator, with a diet dominated by cephalopods (IRI% = 83.68). The two Poroderma spp. showed a partially overlapping, but separate trophic niches, while displaying spatial dissimilarity in diet. The study suggests that the two species are able to coexist within the same geographical area through niche differentiation across trophic and temporal ecological axes, with varying spatial use. The intra- and inter-specific differences between the two species may complicate elasmobranch management efforts for these co-occurring endemic catsharks, and as such, efforts should follow either an individual species approach, which is often not feasible, or an ecosystem-based approach, as opposed to considering the genus as a whole. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-04-05
Genetic connectivity of the slinger Chrysoblephus puniceus among Marine Protected Areas and unprotected areas along the east coast of South Africa
- Ockhuis, Samantha Angelique Natasha
- Authors: Ockhuis, Samantha Angelique Natasha
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435954 , vital:73215 , DOI 10.21504/10962/435954
- Description: Marine Protected Areas (MPAs) are advocated worldwide for the conservation of biodiversity and fisheries management, as well as for adaptation and resilience in the face of climate change. Their effectiveness has been noted through increases in abundances, biomass, sizes and diversity of species, as well as the recovery of overexploited fisheries. For MPAs to realise their full potential, these benefits need to expand beyond their boundaries through larval dispersal and/or adult spillover. Connectivity between MPAs is critical for planning and placement of MPAs, as this promotes the persistence of metapopulations, their recovery from disturbance, as well as productivity in marine ecosystems. The effectiveness of MPAs in conserving biodiversity along the east coast of South Africa has been established; however, limited evidence is available on whether they offer benefits beyond their boundaries, enhancing biodiversity and fisheries in adjacent areas. The slinger Chrysoblephus puniceus, an endemic southern African sparid, is a major component of the commercial linefishery in KwaZulu-Natal (KZN), South Africa and southern Mozambique, and is also caught in the recreational ski-boat fishery in these regions. The spawning of slinger occurs in shoals on offshore reefs from southern Mozambique to the north of KZN, and larvae are likely transported southwards, assisted by the southward-flowing Agulhas Current and promoted by a relatively long larval duration. Slinger are relatively resident, but occasional northward migrations have been observed. These life history characteristics suggested that slinger would be a suitable biological model to test connectivity along the east coast, and particularly connectivity between MPAs. The aim of this study was therefore to investigate connectivity of slinger between MPAs and the surrounding areas along the east coast of South Africa, using restriction-site-associated DNA (RAD) sequencing. This formed part of the larger CAPTOR project investigating east coast MPA connectivity across a range of taxa and habitat types. A temporal comparison of genetic diversity in slinger spanning two sampling events (2012 and 2018) revealed no substantial changes in genetic diversity, as well as a lack of genetic structure along the KZN coast. Similarly, no substantial changes in effective population size (Ne) were found between 2012 and 2018 for microsatellites and Single Nucleotide Polymorphisms (SNPs). However, subtle, albeit non-significant, differences were observed both spatially and over time in the microsatellite data, indicating some instability along the KZN coast. A lack of genetic structure based on RAD sequencing was observed between east coast MPAs and the surrounding areas. This implies high connectivity along the coast - not only are east coast MPAs connected, the surrounding areas are also connected, and there is replenishment between all areas via larval dispersal and/or adult spillover. Analyses of multidirectional gene flow between MPAs, together with known spatial spawning patterns and prior particle dispersal modelling, identified the Richards Bay area as a key source of recruits. However, parentage analysis – a direct method to estimate connectivity – failed to match any parent-offspring pairs in this study; successful parentage assignments are highly dependent on intensive sampling of potential parents and are seldom feasible in marine systems, as most populations have large effective population sizes. Estimates of intergenerational effective population size for slinger in this study are considered large - >2 000 and infinite based on adults and juveniles, respectively. Population genetics revealed close genetic similarities between slinger adult and juvenile slinger sites, emphasizing genetic connectivity and the lack of structure between sites observed before. This was the first study on South African sparids to use RAD sequencing to assess genetic connectivity between MPAs along the east coast of South Africa. Overall, results provide evidence that MPAs along the east coast of South Africa are connected, and can readily replenish or be replenished by other sites along the east coast of South Africa, whether they are within MPAs or not. So too are the areas surrounding MPAs interconnected, and slinger forms a homogenous single population on the east coast of South Africa. This notwithstanding, it is important that east coast MPAs, particularly those that host spawning shoals of slinger, are well managed in order that they continue to provide recruits to sustain the population and support sustainable fisheries. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-04-05
- Authors: Ockhuis, Samantha Angelique Natasha
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435954 , vital:73215 , DOI 10.21504/10962/435954
- Description: Marine Protected Areas (MPAs) are advocated worldwide for the conservation of biodiversity and fisheries management, as well as for adaptation and resilience in the face of climate change. Their effectiveness has been noted through increases in abundances, biomass, sizes and diversity of species, as well as the recovery of overexploited fisheries. For MPAs to realise their full potential, these benefits need to expand beyond their boundaries through larval dispersal and/or adult spillover. Connectivity between MPAs is critical for planning and placement of MPAs, as this promotes the persistence of metapopulations, their recovery from disturbance, as well as productivity in marine ecosystems. The effectiveness of MPAs in conserving biodiversity along the east coast of South Africa has been established; however, limited evidence is available on whether they offer benefits beyond their boundaries, enhancing biodiversity and fisheries in adjacent areas. The slinger Chrysoblephus puniceus, an endemic southern African sparid, is a major component of the commercial linefishery in KwaZulu-Natal (KZN), South Africa and southern Mozambique, and is also caught in the recreational ski-boat fishery in these regions. The spawning of slinger occurs in shoals on offshore reefs from southern Mozambique to the north of KZN, and larvae are likely transported southwards, assisted by the southward-flowing Agulhas Current and promoted by a relatively long larval duration. Slinger are relatively resident, but occasional northward migrations have been observed. These life history characteristics suggested that slinger would be a suitable biological model to test connectivity along the east coast, and particularly connectivity between MPAs. The aim of this study was therefore to investigate connectivity of slinger between MPAs and the surrounding areas along the east coast of South Africa, using restriction-site-associated DNA (RAD) sequencing. This formed part of the larger CAPTOR project investigating east coast MPA connectivity across a range of taxa and habitat types. A temporal comparison of genetic diversity in slinger spanning two sampling events (2012 and 2018) revealed no substantial changes in genetic diversity, as well as a lack of genetic structure along the KZN coast. Similarly, no substantial changes in effective population size (Ne) were found between 2012 and 2018 for microsatellites and Single Nucleotide Polymorphisms (SNPs). However, subtle, albeit non-significant, differences were observed both spatially and over time in the microsatellite data, indicating some instability along the KZN coast. A lack of genetic structure based on RAD sequencing was observed between east coast MPAs and the surrounding areas. This implies high connectivity along the coast - not only are east coast MPAs connected, the surrounding areas are also connected, and there is replenishment between all areas via larval dispersal and/or adult spillover. Analyses of multidirectional gene flow between MPAs, together with known spatial spawning patterns and prior particle dispersal modelling, identified the Richards Bay area as a key source of recruits. However, parentage analysis – a direct method to estimate connectivity – failed to match any parent-offspring pairs in this study; successful parentage assignments are highly dependent on intensive sampling of potential parents and are seldom feasible in marine systems, as most populations have large effective population sizes. Estimates of intergenerational effective population size for slinger in this study are considered large - >2 000 and infinite based on adults and juveniles, respectively. Population genetics revealed close genetic similarities between slinger adult and juvenile slinger sites, emphasizing genetic connectivity and the lack of structure between sites observed before. This was the first study on South African sparids to use RAD sequencing to assess genetic connectivity between MPAs along the east coast of South Africa. Overall, results provide evidence that MPAs along the east coast of South Africa are connected, and can readily replenish or be replenished by other sites along the east coast of South Africa, whether they are within MPAs or not. So too are the areas surrounding MPAs interconnected, and slinger forms a homogenous single population on the east coast of South Africa. This notwithstanding, it is important that east coast MPAs, particularly those that host spawning shoals of slinger, are well managed in order that they continue to provide recruits to sustain the population and support sustainable fisheries. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-04-05
Review of the systematics and regional diversity of the diploid barbs (Enteromius Cope, 1867) of southern Africa
- Scheepers, Martinus Johannes
- Authors: Scheepers, Martinus Johannes
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435966 , vital:73216
- Description: Access restricted. Expected release in 2025. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-04-05
- Authors: Scheepers, Martinus Johannes
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435966 , vital:73216
- Description: Access restricted. Expected release in 2025. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-04-05
The effect of Gracilaria gracilis (Rhodophyta) on growth and behaviour of farmed abalone Haliotis midae when included in the abalone diet
- Madlala, Njabulo Praiseworth
- Authors: Madlala, Njabulo Praiseworth
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435332 , vital:73148
- Description: Abalone feed naturally on algae, but commercial abalone farms rely on formulated feed. The inclusion of algae with the formulated feed in the diet improves growth, health and feed conversion ratio (FCR). However, the kelp that is currently included in the feed is wild harvested and this resource is limited and under increasing pressure. Thus, there is a need to develop an alternative sustainable source of algae. The aim was to use Gracilaria gracilis algae (that was produced with mussel in an integrated multitrophic aquaculture system; IMTA) which was then included into abalone feeds and its influence on abalone growth, FCR and behaviour was evaluated. Sea-based G. gracilis was harvested off an existing IMTA system, manufactured into Abfeed® and fed to abalone to determine growth efficiency. Abalone (30 - 40 g abalone-1) originated from the same cohort at Whale Rock Abalone Farm (Pty) Ltd., Hermanus. Abalone were fed either: Abfeed® S34 only with no algae (G0, the control); Abfeed® with sea-based IMTA G. gracilis included in the formulated feed at 0.75 % (G0.75); 1.50 % (G1.50); 3.00 % (G3.00); 6.00 % (G6.00); and 12.00 % (G12.00) inclusion; and Abfeed® S34 with fresh-live G. gracilis (cultured at Whale Rock Abalone Farm (Pty) Ltd.; Gfresh + S34s). Abalone shell length, whole body mass and FCR were measured at a four-months interval over eight months (two growth cycles). Differences in behaviour (i.e., level of activity and feeding activity) were also monitored and comparing between treatments. There was significant difference in mean whole-body mass between treatments (RMANOVA, F(6, 29) = 3.71, p = 0.007). Abalone fed the diet with the highest inclusion of dry G. gracilis (G12.00) had a lower mean whole-body mass value (74.13 ± 2.94 g abalone-1) than abalone from all the other diets after eight months. There was a negative relationship between the condition factor and dry G. gracilis inclusion rate for the first growth cycle (y = - 0.0044x + 1.222, R2 = 0.24, p=0.009) and second-growth cycle (y = - 0.0096x + 1.2233, R2 = 0.51, p = 0.00002). Abalone receiving fresh G. gracilis were less quiescent and more alert on the first hours after food distribution (p < 0.05). The same proportion of abalone with G0, G0.75, and G6.00 G. gracilis inclusion were feeding all along the 9 hours post-feeding. This study will potentially reduce the dependence of abalone farms on wild-harvested kelp in formulated feed. This will improve their contribution to a more environmentally sustainable global production of sea food products, making them more competitive on international markets. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Madlala, Njabulo Praiseworth
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435332 , vital:73148
- Description: Abalone feed naturally on algae, but commercial abalone farms rely on formulated feed. The inclusion of algae with the formulated feed in the diet improves growth, health and feed conversion ratio (FCR). However, the kelp that is currently included in the feed is wild harvested and this resource is limited and under increasing pressure. Thus, there is a need to develop an alternative sustainable source of algae. The aim was to use Gracilaria gracilis algae (that was produced with mussel in an integrated multitrophic aquaculture system; IMTA) which was then included into abalone feeds and its influence on abalone growth, FCR and behaviour was evaluated. Sea-based G. gracilis was harvested off an existing IMTA system, manufactured into Abfeed® and fed to abalone to determine growth efficiency. Abalone (30 - 40 g abalone-1) originated from the same cohort at Whale Rock Abalone Farm (Pty) Ltd., Hermanus. Abalone were fed either: Abfeed® S34 only with no algae (G0, the control); Abfeed® with sea-based IMTA G. gracilis included in the formulated feed at 0.75 % (G0.75); 1.50 % (G1.50); 3.00 % (G3.00); 6.00 % (G6.00); and 12.00 % (G12.00) inclusion; and Abfeed® S34 with fresh-live G. gracilis (cultured at Whale Rock Abalone Farm (Pty) Ltd.; Gfresh + S34s). Abalone shell length, whole body mass and FCR were measured at a four-months interval over eight months (two growth cycles). Differences in behaviour (i.e., level of activity and feeding activity) were also monitored and comparing between treatments. There was significant difference in mean whole-body mass between treatments (RMANOVA, F(6, 29) = 3.71, p = 0.007). Abalone fed the diet with the highest inclusion of dry G. gracilis (G12.00) had a lower mean whole-body mass value (74.13 ± 2.94 g abalone-1) than abalone from all the other diets after eight months. There was a negative relationship between the condition factor and dry G. gracilis inclusion rate for the first growth cycle (y = - 0.0044x + 1.222, R2 = 0.24, p=0.009) and second-growth cycle (y = - 0.0096x + 1.2233, R2 = 0.51, p = 0.00002). Abalone receiving fresh G. gracilis were less quiescent and more alert on the first hours after food distribution (p < 0.05). The same proportion of abalone with G0, G0.75, and G6.00 G. gracilis inclusion were feeding all along the 9 hours post-feeding. This study will potentially reduce the dependence of abalone farms on wild-harvested kelp in formulated feed. This will improve their contribution to a more environmentally sustainable global production of sea food products, making them more competitive on international markets. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2024
- Full Text:
- Date Issued: 2024-04-04
Comparison of the metabolic physiology of exploited and unexploited populations of red roman (Chrysoblephus laticeps) along the south coast of South Africa
- Authors: Nabani, Xolani Prince
- Date: 2023-10-13
- Subjects: Chrysoblephus laticeps , Marine fishes Climatic factors South Africa , Marine fishes Physiology South Africa , Marine fishes Metabolism , Respirometry , Marine protected area , Evolutionary impact of fishing
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424358 , vital:72146
- Description: Anthropogenic-induced climate change and exploitation pose threat to many marine fishes on which a vast majority of people around the world depend. Rapid changes in sea surface temperature have a direct impact on the physiology of ectothermic organisms such as fish, potentially resulting in changes to population distribution, abundance, and demographics. In the face of climate change, the impacts of increasing temperature variability on fish populations may be exacerbated by exploitation. Understanding how the resilience of exploited populations is affected by climate change is critical to predict how fishes will respond in the future. This study aimed to augment our knowledge on the impact of exploitation and thermal variability on fishes by comparing the thermal physiology of an exploited and unexploited population of the resident, reef-dwelling, Chrysoblephus laticeps. Twenty live fish were collected from the exploited, Cape St Francis and 18 fish from the unexploited, Goukamma Marine Protected Area and transported to the laboratory. The metabolic performance, in terms of standard metabolic rate (SMR), maximum metabolic rate (MMR) and aerobic scope (AS) of individual C. laticeps were estimated repeatedly at 10 ℃, 16 °C and 21 °C. Linear mixed effects models were used to examine the relationship between temperature, population, and metabolic rate and a ‘cvequality’ test analysis was used to compare the variance structure of the metabolic rate regression model for each population. Overall, the findings of this study show that Chrysoblephus laticeps from the unexploited population maintains a significantly higher aerobic scope (AS) across all temperature treatments (10, 16 and 21 ℃) when compared with those from the exploited population. In addition, the maximum metabolic rate (MMR) of individuals from the unexploited population was significantly higher than that of individuals from the exploited population, but there was no evidence to suggest that variability was significantly different between the populations. On the other hand, the individuals from an exploited population had a significantly higher standard metabolic rate (SMR) at high temperatures of 21 ℃, while the unexploited population had a low SMR at these high temperatures, but a high SMR at 10 ℃. Despite these differences there was no significant variation in the SMR between the two populations. The findings of this study confirm previous work on different exploited and unexploited populations of C. laticeps and together these findings suggest that hook and line exploitation lead to reduced physiological phenotypic diversity and reduced physiological performance in exploited fish populations. These findings emphasise the importance of incorporating the iii physiological information to develop viable fisheries management tools in the context of climate change. This study also highlights the effectiveness of MPAs in conserving highperformance physiological phenotypes to maintain phenotypic diversity in fish populations. Future research should aim to evaluate the efficacy of existing MPAs in preserving the physiological diversity of important hook and line fisheries species, while fisheries managers should consider augmenting their approaches through the incorporation of well-designed MPA’s to promote physiological diversity. This will be critical to advance the development of sustainable management practices, not only in a South African context but globally, where oceanic and coastal environmental conditions are expected to rapidly change in the future. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Nabani, Xolani Prince
- Date: 2023-10-13
- Subjects: Chrysoblephus laticeps , Marine fishes Climatic factors South Africa , Marine fishes Physiology South Africa , Marine fishes Metabolism , Respirometry , Marine protected area , Evolutionary impact of fishing
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424358 , vital:72146
- Description: Anthropogenic-induced climate change and exploitation pose threat to many marine fishes on which a vast majority of people around the world depend. Rapid changes in sea surface temperature have a direct impact on the physiology of ectothermic organisms such as fish, potentially resulting in changes to population distribution, abundance, and demographics. In the face of climate change, the impacts of increasing temperature variability on fish populations may be exacerbated by exploitation. Understanding how the resilience of exploited populations is affected by climate change is critical to predict how fishes will respond in the future. This study aimed to augment our knowledge on the impact of exploitation and thermal variability on fishes by comparing the thermal physiology of an exploited and unexploited population of the resident, reef-dwelling, Chrysoblephus laticeps. Twenty live fish were collected from the exploited, Cape St Francis and 18 fish from the unexploited, Goukamma Marine Protected Area and transported to the laboratory. The metabolic performance, in terms of standard metabolic rate (SMR), maximum metabolic rate (MMR) and aerobic scope (AS) of individual C. laticeps were estimated repeatedly at 10 ℃, 16 °C and 21 °C. Linear mixed effects models were used to examine the relationship between temperature, population, and metabolic rate and a ‘cvequality’ test analysis was used to compare the variance structure of the metabolic rate regression model for each population. Overall, the findings of this study show that Chrysoblephus laticeps from the unexploited population maintains a significantly higher aerobic scope (AS) across all temperature treatments (10, 16 and 21 ℃) when compared with those from the exploited population. In addition, the maximum metabolic rate (MMR) of individuals from the unexploited population was significantly higher than that of individuals from the exploited population, but there was no evidence to suggest that variability was significantly different between the populations. On the other hand, the individuals from an exploited population had a significantly higher standard metabolic rate (SMR) at high temperatures of 21 ℃, while the unexploited population had a low SMR at these high temperatures, but a high SMR at 10 ℃. Despite these differences there was no significant variation in the SMR between the two populations. The findings of this study confirm previous work on different exploited and unexploited populations of C. laticeps and together these findings suggest that hook and line exploitation lead to reduced physiological phenotypic diversity and reduced physiological performance in exploited fish populations. These findings emphasise the importance of incorporating the iii physiological information to develop viable fisheries management tools in the context of climate change. This study also highlights the effectiveness of MPAs in conserving highperformance physiological phenotypes to maintain phenotypic diversity in fish populations. Future research should aim to evaluate the efficacy of existing MPAs in preserving the physiological diversity of important hook and line fisheries species, while fisheries managers should consider augmenting their approaches through the incorporation of well-designed MPA’s to promote physiological diversity. This will be critical to advance the development of sustainable management practices, not only in a South African context but globally, where oceanic and coastal environmental conditions are expected to rapidly change in the future. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
Exploring the drivers of co-occurring multiple non-native fish assemblages within an invaded and flow-modified African river system
- Authors: Mpopetsi, Pule Peter
- Date: 2023-10-13
- Subjects: Freshwater ecology , Invasion biology , Freshwater fishes South Africa Great Fish River Estuary , Functional trait , Functional diversity , Introduced fishes South Africa Great Fish River Estuary , Food chains (Ecology)
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431871 , vital:72810 , DOI 10.21504/10962/431871
- Description: Globally, there is growing concern on the negative impacts of species invasions and habitat disturbance because these have been shown to have the potential to disrupt native community structure and function. In some instances, these two stressors can occur in concert, such as in river systems associated with inter-basin water transfer (IBWT) schemes. The Great Fish River in the Eastern Cape, South Africa, is an example of a system affected by both habitat modification and multiple fish invasions largely because of an IBWT scheme. The opening of the Orange-Fish IBWT, which transfers water from the Orange River to the Great Fish River, modified the latter’s natural flow regime from irregular seasonal to perennial. In addition, the IBWT facilitated translocations of five fish species from the Gariep Dam (Orange River system) into the Great Fish River system. Proliferation of these non-native fish species, along with that of other fish species introduced for angling and biological control, raise questions on the mechanisms facilitating their existence within this highly modified river system. This thesis explored mechanisms associated with co-occurrences of these multiple non-native fishes within the Great Fish River. A comparison of historical and contemporary records on the ichthyofauna of the Great Fish River revealed that, of the 11 non-native fishes reported in this system, seven have established successfully, three have failed to establish and the status of one was uncertain. The Orange-Fish IBWT and angling were the main vectors of these invasions, accounting for 36% and 46%, respectively. The study also found that most established non-native fish species were large sized, had high longevity and wide habitat tolerance. Trait-based approaches were employed to investigate the role of functional diversity of non-native and native fishes in relation to their composition, distribution and environmental relationships. Although considerable interspecific variation in body morphology-related functional traits among species were observed, there was no clear distinction in these traits between native and non-native fish assemblages on a trait-ordination space. Furthermore, there were weak species-trait-environment relationships, suggesting that environmental filtering was less plausible in explaining the occurrence patterns of these fishes. Stable isotope-based trophic relationships were evaluated in three invaded sections: the upper (UGFR) mainstem sections of the Great Fish River; and lower (LGFR) mainstem sections of the Great Fish River; and its tributary, the Koonap River. It was observed that native and non-native fish assemblages exhibited variation in isotopic diversity typified by low isotopic diversity overlaps in UGFR and Koonap River, whereas the LGFR was characterised by high isotopic diversity overlap. Within the invaded sections, non-native fishes were found to have isotopic niches characterised by variable isotopic niche sizes and were more isotopically dissimilar with propensity towards trophic differentiation within the UGFR and Koonap River but were mostly characterised by high isotope niche overlaps in the LGFR. Overall, these results provided evidence of trophic niche differentiation as a probable mechanism associated with the co-occurrences of the non-native fishes. However, mechanisms facilitating these co-occurrences within the invaded sections appears to be complex, context-specific and, in some cases, unclear. Lastly, machine learning techniques, boosted (BRT) and multivariate (MRT) regression trees, revealed that the flow-disturbed habitats were invaded by multiple non-native species, whereas the non-disturbed headwaters remained invasion free. In addition, non-native species were predicted to co-occur with native species within the mainstem and large tributary sections of the Great Fish River system. Thus, the IBWT-disturbed mainstem sections were predicted to be more prone to multiple invasions compared to undisturbed headwater tributaries. , Tlhaselo ka mefuta ya diphoofolo-tsa-matswantle (non-native species), ha mmoho le phetolo/tsenyehelo ya bodulo ba diphoofolo-tsa-lehae (native species), di nkuwa ele tse pedi tsa tse kgolo ka ho fetisisa hara ditshoso tse kgahlanong le paballo kapa tshireletso ya diphoofolo-tsa-lehae tse phelang dinokeng kapa metsing. Maemong a mang, dikgatello tsena tse pedi dika etsahala ka nako e le nngwe, jwalo ka dinokeng tseo di amanang le maano a ho fetisa/tsamaisa metsi pakeng tsa dinoka tse fapa-fapaneng (IBWT). Enngwe ya dinoka tse jwalo, ke noka e bitswang ka Great Fish River, e fumanehang Kapa-Botjabela (Eastern Cape) ka hara naha ya Afrika Borwa (South Africa). Noka ena ya Great Fish River e angwa ke tshenyehelo ya bodulo ba ditlhapi-tsa-lehae, ha mmoho le tlhaselo ya tsona ka ditlhapi-tsa-matswantle. Tsena di etsahala hahololo ka lebaka la morero kapa leano la phepelo ya metsi le bitswang Orange-Fish IBWT, leo lona le ileng la fetola phallo ya tlhaho ya metsi a Great Fish River. Ho feta moo, leano lena la phephelo yametsi, Orange-Fish IBWT, le entse hore ho be bonolo ho fetisetswa ha mefuta e mehlano ya ditlhapi-tsa-matswantle ho tloha letamong le bitswang Gariep Dam, hoya kena ka hara noka ya Great Fish River. Ditla morao tsa tsena tsohle, ebile ho ata ha mefuta e mengata ya ditlhapi-tsa-matswantle ka hara noka ya Great Fish River. Ho ata hona ha ditlapi-tsa-matswantle ka hara noka ena ya Great Fish River, ho hlahisa dipotso mabapi le mekgwa e bebofatsang ho phela ha ditlhapi tsena tsa matswantle ka hara noka ena; hore ana ebe diphela jwang ka hara noka ya Great Fish River? Ka hona, sepheo le merero wa thuto ena ke ho phuputsa mekgwa e bebofatsang ho phela ha mefuta ena e fapaneng ya ditlhapi-tsa-matswantle ka hara noka ya Great Fish River. Dipheto tsa diphuputso di hlalosa hore, ha jwale, ka hara noka ena ya Great Fish River, hona le ditlhapi-tsa-matswantle tse leshome le motso o mong (11). Bosupa (7) ba tsona di phela ka katleho, ha tse tharo di hlolehile ho theha (3), mme e le nngwe (1) boemo ba teng ha bo hlake. Hare lekola hore ke efeng mekgwa e amanang le ho ata ha ditlhapi-tsa-matswantle ka hara Great Fish River, re fumana hore leano la phephelo ya metsi la Orange-Fish IBWT ka 36%, ha mmoho le boithapollo ba ho tshwasa ditlhapi (angling) ka 46%, ene ele tsona tsela tsa ho kena ha ditlhapi-tsa-matswantle ka hara Great Fish River, tse ka sehlohong. Re fumantsha hape hore katleho ya ditlhapi-tsa-matswantle e amahangwa le hore di boholo bo bokae, le hore diphela nako e ka kang. Mohlala, ditlhapi tse kgolo tse phelang nako etelele ka tlhaho ya tsona, di amahangwa le katleho ya ho theha ka hara noka ena. Ha tseo tse phelang nako e kgutshwanyane tsona disa amahangwe leho atleha ka hara noka ena. Tse ding tsa dipheto di hlalosa hore, ditlhapi-tsa-lehae le ditlhapi-tsa-matswantle, ka karolelano, hadi fapane haholo ka dibopeho tsa mmele, dihlopa tsena tse pedi diya tshwana. Re fumantsha hape hore dihlopa tsena tse pedi tsa ditlapi dija mefuta e fapaneng ya dijo. Eleng engwe ya dintho tse netefatsang katleho ya ditlhapi-tsa-matswantle ka hara noka ena ya Great fish river. Hona keka lebaka la hore, dihlopa tsena tse pedi hadi bakisane dijo, empa di phela ka mefuta e fapaneng ya dijo. Hare phethela, re fumantsha hore mefuta e fapafapaneng ya ditlhapi-tsa-matswantle e fumaneha feela ka hara madulo a amahangwang le phethoho ya phallo ya metsi (flow alteration), madulo asa amahangwang le phetoho ya phallo ya metsi ona ane a hloka ditlhapi-tsa-matswantle. Sena se bolela hore phetolo ya phallo ya metsi ya Great Fish River, ka lebaka la Orange-Fish IBWT, e fokoditse matla a noka ena ho lwantsha tlhaselo ya ditlhapi-tsa-matswantle. Ka hona, ho bobebe hore ditlhapi-tsa-matswantle di thehe ka katleho ka hara noka ena. Tsena tsohle keka baka la phetolo ya phallo ya metsi a Great Fish River e bakilweng ke leano la phephelo ya metsi la Orange-Fish IBWT. , Thesis (PhD) -- Faculty of Science, Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Mpopetsi, Pule Peter
- Date: 2023-10-13
- Subjects: Freshwater ecology , Invasion biology , Freshwater fishes South Africa Great Fish River Estuary , Functional trait , Functional diversity , Introduced fishes South Africa Great Fish River Estuary , Food chains (Ecology)
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431871 , vital:72810 , DOI 10.21504/10962/431871
- Description: Globally, there is growing concern on the negative impacts of species invasions and habitat disturbance because these have been shown to have the potential to disrupt native community structure and function. In some instances, these two stressors can occur in concert, such as in river systems associated with inter-basin water transfer (IBWT) schemes. The Great Fish River in the Eastern Cape, South Africa, is an example of a system affected by both habitat modification and multiple fish invasions largely because of an IBWT scheme. The opening of the Orange-Fish IBWT, which transfers water from the Orange River to the Great Fish River, modified the latter’s natural flow regime from irregular seasonal to perennial. In addition, the IBWT facilitated translocations of five fish species from the Gariep Dam (Orange River system) into the Great Fish River system. Proliferation of these non-native fish species, along with that of other fish species introduced for angling and biological control, raise questions on the mechanisms facilitating their existence within this highly modified river system. This thesis explored mechanisms associated with co-occurrences of these multiple non-native fishes within the Great Fish River. A comparison of historical and contemporary records on the ichthyofauna of the Great Fish River revealed that, of the 11 non-native fishes reported in this system, seven have established successfully, three have failed to establish and the status of one was uncertain. The Orange-Fish IBWT and angling were the main vectors of these invasions, accounting for 36% and 46%, respectively. The study also found that most established non-native fish species were large sized, had high longevity and wide habitat tolerance. Trait-based approaches were employed to investigate the role of functional diversity of non-native and native fishes in relation to their composition, distribution and environmental relationships. Although considerable interspecific variation in body morphology-related functional traits among species were observed, there was no clear distinction in these traits between native and non-native fish assemblages on a trait-ordination space. Furthermore, there were weak species-trait-environment relationships, suggesting that environmental filtering was less plausible in explaining the occurrence patterns of these fishes. Stable isotope-based trophic relationships were evaluated in three invaded sections: the upper (UGFR) mainstem sections of the Great Fish River; and lower (LGFR) mainstem sections of the Great Fish River; and its tributary, the Koonap River. It was observed that native and non-native fish assemblages exhibited variation in isotopic diversity typified by low isotopic diversity overlaps in UGFR and Koonap River, whereas the LGFR was characterised by high isotopic diversity overlap. Within the invaded sections, non-native fishes were found to have isotopic niches characterised by variable isotopic niche sizes and were more isotopically dissimilar with propensity towards trophic differentiation within the UGFR and Koonap River but were mostly characterised by high isotope niche overlaps in the LGFR. Overall, these results provided evidence of trophic niche differentiation as a probable mechanism associated with the co-occurrences of the non-native fishes. However, mechanisms facilitating these co-occurrences within the invaded sections appears to be complex, context-specific and, in some cases, unclear. Lastly, machine learning techniques, boosted (BRT) and multivariate (MRT) regression trees, revealed that the flow-disturbed habitats were invaded by multiple non-native species, whereas the non-disturbed headwaters remained invasion free. In addition, non-native species were predicted to co-occur with native species within the mainstem and large tributary sections of the Great Fish River system. Thus, the IBWT-disturbed mainstem sections were predicted to be more prone to multiple invasions compared to undisturbed headwater tributaries. , Tlhaselo ka mefuta ya diphoofolo-tsa-matswantle (non-native species), ha mmoho le phetolo/tsenyehelo ya bodulo ba diphoofolo-tsa-lehae (native species), di nkuwa ele tse pedi tsa tse kgolo ka ho fetisisa hara ditshoso tse kgahlanong le paballo kapa tshireletso ya diphoofolo-tsa-lehae tse phelang dinokeng kapa metsing. Maemong a mang, dikgatello tsena tse pedi dika etsahala ka nako e le nngwe, jwalo ka dinokeng tseo di amanang le maano a ho fetisa/tsamaisa metsi pakeng tsa dinoka tse fapa-fapaneng (IBWT). Enngwe ya dinoka tse jwalo, ke noka e bitswang ka Great Fish River, e fumanehang Kapa-Botjabela (Eastern Cape) ka hara naha ya Afrika Borwa (South Africa). Noka ena ya Great Fish River e angwa ke tshenyehelo ya bodulo ba ditlhapi-tsa-lehae, ha mmoho le tlhaselo ya tsona ka ditlhapi-tsa-matswantle. Tsena di etsahala hahololo ka lebaka la morero kapa leano la phepelo ya metsi le bitswang Orange-Fish IBWT, leo lona le ileng la fetola phallo ya tlhaho ya metsi a Great Fish River. Ho feta moo, leano lena la phephelo yametsi, Orange-Fish IBWT, le entse hore ho be bonolo ho fetisetswa ha mefuta e mehlano ya ditlhapi-tsa-matswantle ho tloha letamong le bitswang Gariep Dam, hoya kena ka hara noka ya Great Fish River. Ditla morao tsa tsena tsohle, ebile ho ata ha mefuta e mengata ya ditlhapi-tsa-matswantle ka hara noka ya Great Fish River. Ho ata hona ha ditlapi-tsa-matswantle ka hara noka ena ya Great Fish River, ho hlahisa dipotso mabapi le mekgwa e bebofatsang ho phela ha ditlhapi tsena tsa matswantle ka hara noka ena; hore ana ebe diphela jwang ka hara noka ya Great Fish River? Ka hona, sepheo le merero wa thuto ena ke ho phuputsa mekgwa e bebofatsang ho phela ha mefuta ena e fapaneng ya ditlhapi-tsa-matswantle ka hara noka ya Great Fish River. Dipheto tsa diphuputso di hlalosa hore, ha jwale, ka hara noka ena ya Great Fish River, hona le ditlhapi-tsa-matswantle tse leshome le motso o mong (11). Bosupa (7) ba tsona di phela ka katleho, ha tse tharo di hlolehile ho theha (3), mme e le nngwe (1) boemo ba teng ha bo hlake. Hare lekola hore ke efeng mekgwa e amanang le ho ata ha ditlhapi-tsa-matswantle ka hara Great Fish River, re fumana hore leano la phephelo ya metsi la Orange-Fish IBWT ka 36%, ha mmoho le boithapollo ba ho tshwasa ditlhapi (angling) ka 46%, ene ele tsona tsela tsa ho kena ha ditlhapi-tsa-matswantle ka hara Great Fish River, tse ka sehlohong. Re fumantsha hape hore katleho ya ditlhapi-tsa-matswantle e amahangwa le hore di boholo bo bokae, le hore diphela nako e ka kang. Mohlala, ditlhapi tse kgolo tse phelang nako etelele ka tlhaho ya tsona, di amahangwa le katleho ya ho theha ka hara noka ena. Ha tseo tse phelang nako e kgutshwanyane tsona disa amahangwe leho atleha ka hara noka ena. Tse ding tsa dipheto di hlalosa hore, ditlhapi-tsa-lehae le ditlhapi-tsa-matswantle, ka karolelano, hadi fapane haholo ka dibopeho tsa mmele, dihlopa tsena tse pedi diya tshwana. Re fumantsha hape hore dihlopa tsena tse pedi tsa ditlapi dija mefuta e fapaneng ya dijo. Eleng engwe ya dintho tse netefatsang katleho ya ditlhapi-tsa-matswantle ka hara noka ena ya Great fish river. Hona keka lebaka la hore, dihlopa tsena tse pedi hadi bakisane dijo, empa di phela ka mefuta e fapaneng ya dijo. Hare phethela, re fumantsha hore mefuta e fapafapaneng ya ditlhapi-tsa-matswantle e fumaneha feela ka hara madulo a amahangwang le phethoho ya phallo ya metsi (flow alteration), madulo asa amahangwang le phetoho ya phallo ya metsi ona ane a hloka ditlhapi-tsa-matswantle. Sena se bolela hore phetolo ya phallo ya metsi ya Great Fish River, ka lebaka la Orange-Fish IBWT, e fokoditse matla a noka ena ho lwantsha tlhaselo ya ditlhapi-tsa-matswantle. Ka hona, ho bobebe hore ditlhapi-tsa-matswantle di thehe ka katleho ka hara noka ena. Tsena tsohle keka baka la phetolo ya phallo ya metsi a Great Fish River e bakilweng ke leano la phephelo ya metsi la Orange-Fish IBWT. , Thesis (PhD) -- Faculty of Science, Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
Movement ecology of a West African sciaenid fish, Argyrosomus coronus, in southern Angola
- Authors: Parkinson, Matthew Cameron
- Date: 2023-10-13
- Subjects: Argyrosomus coronus Benguela Current Geographical distribution , Underwater acoustic telemetry , Fisheries Benguela Current , Fish populations , Spatio-temporal dynamics , Spatial ecology
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432203 , vital:72851 , DOI 10.21504/10962/432203
- Description: Argyrosomus coronus is a large sciaenid species with a primary distribution between Cape Frio, in Namibia, and Luanda, in Angola, where it exists as a panmictic stock. Early juveniles (< 300 mm TL) occur on muddy offshore substrata (50–80 m deep) and at one year of age they recruit into the inshore zone. Adults are thought to predominantly occur inshore. Spawning occurs in the species during late spring. The species is heavily targeted by the recreational, subsistence, artisanal and commercial fisheries as juveniles through to adults and there are signs of population decline with declines in catch per unit effort (CPUE) and maximum size. A basic understanding of their movement ecology has emerged from previous studies, based on conventional tagging (mark-recapture) and CPUE monitoring from a shore-based recreational fishery. Juveniles were thought to be resident, with larger fish undertaking long distance migration southward in the austral summer and returning during the austral winter. In addition to the fishery-related threats faced by the species, the southern Angolan region has been identified to be an ocean warming hotspot, and this has been linked to a southward distribution shift and the recent hybridisation of A. coronus with its congener A. inodorus, in Namibia. This study aims to expand the knowledge of the movement ecology of A. coronus and to interrogate our current understanding of the movement patterns of the species using passive acoustic telemetry. Passive acoustic receivers were deployed at three study sites, Flamingo, where all tagging occurred, which lies ~ 200 km north of the Angolan border with Namibia, is a relatively exposed stretch of coastline; Tombua Bay, which lies 30 km south of Flamingo, is a small, sheltered natural embayment; and Baia dos Tigres, which lies 100 km south of Tombua Bay, is a sheltered lagoon in the lee of an island ~ 10 km from the mainland. Tagging occurred in two batches, one year apart. In the first batch, sub-adults (n = 3) and adults (n = 17) were tagged and monitored for two years. In the second batch, juveniles (n = 7) and sub-adults (n = 3) were tagged and monitored for one year. The first objective of this thesis (Chapter 3) was to examine the spatio-temporal dynamics of A. coronus at a regional-level (across study sites, to investigate the prevalence of the longshore migration), and at a local-level (within a study site), and to categorise the movement behaviour of juveniles, sub-adults and adults. Traditional seasons were not used in this thesis, as the study region alternates between ‘warm’ periods (up to ~ 26 °C mean daily water temperature), when Angola Current water covers the area, and ‘cold’ periods (down to ~ 15 °C mean daily water temperature), when Benguela Current water covers the area. A high degree of residency of tagged fish to the Flamingo study site, where tagging was conducted, was found, with juveniles and sub-adults never being detected outside of the Flamingo study site. Five adults (29 %) were detected haphazardly for between one and 36 days at Tombua Bay, following which they were typically detected again at the Flamingo study site. No fish were ever detected at the southernmost site, Baia dos Tigres, suggesting that none migrated to Namibia. Within the Flamingo study site, adults were found to group at the inshore mid-region of the study site during ‘cold’ periods, dispersing again during ‘warm’ periods, when there was a concomitant offshore shift in their area use. This explained the absence of the species from catches in the shore-based recreational fishery during ‘warm’ periods. These results challenge previous migration hypotheses for the species. Therefore, longshore return migrations which were evident from previously conducted conventional tagging, are probably not the norm for the species, but likely occur at a low frequency. The species exhibited a high degree of residency to their tagging site, despite the drastic seasonal changes in water temperatures and station-keeping was the dominant behaviour across all life stages. The constrained area-use noted in this study, relative to the known distribution of the species, suggests that A. coronus exists as a metapopulation, consisting of a network of subpopulations interconnected by gene flow that is most likely facilitated during their pelagic egg and larval phase. The second objective of this thesis was to examine the group formation observed at the Flamingo study site during ‘cold’ periods (Chapter 4). While this grouping of individuals was found to be correlated with water temperature, it was unlikely to be a causal relationship, as the water temperature was not dissimilar to adjacent areas. The area where individuals were concentrated is known to frequently attract large shoals of their dominant prey, Sardinella aurita. While the timing of this group formation aligns with a pre-spawning period, no studies have assessed the existence of local spawning. A. coronus were, however, likely utilising an abundant prey source, during the important pre-spawning period, in order to build up energy reserves. Group formation is a common feature of sciaenids, but literature on the subject is restricted to spawning congregations. Fish are not only more vulnerable to capture during these periods but may also be disturbed during these potentially important social periods. The third objective of this thesis was to investigate the presence of sociality in the species (Chapter 5). Evidence for sociality during and outside the seasonal group formation was explored using network analyses. There was evidence for sociality, with several groups, characterized by co-locations among individuals, identified. While these groups were fluid through time, there was evidence of persistent sociality, with two individuals in particular being consistently co-located over the entire study period. Due to the persistent nature of sociality, group foraging is suggested as an explanation for this, with anecdotal evidence of coordinated hunting supporting this. Acoustic telemetry vastly improved the knowledge of the movement ecology of A. coronus. Despite their panmictic population genetic structure, they were found to be largely resident, likely occurring as a metapopulation across their distribution, with egg and larval dispersal likely the primary mechanism for maintaining panmixia. Evidence for sociality was also found, which was previously unexplored in the species. The persistent nature of social groupings throughout the year, suggests that it may be linked with group foraging and is supported by anecdotal evidence of coordinated hunting by the species. While this study highlights the value of acoustic telemetry in studying movement ecology, it also shows the importance of drawing on multiple methods to fully understand a species’ spatial ecology, including mark-recapture, population genetics, and fishery catch and effort data. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Parkinson, Matthew Cameron
- Date: 2023-10-13
- Subjects: Argyrosomus coronus Benguela Current Geographical distribution , Underwater acoustic telemetry , Fisheries Benguela Current , Fish populations , Spatio-temporal dynamics , Spatial ecology
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432203 , vital:72851 , DOI 10.21504/10962/432203
- Description: Argyrosomus coronus is a large sciaenid species with a primary distribution between Cape Frio, in Namibia, and Luanda, in Angola, where it exists as a panmictic stock. Early juveniles (< 300 mm TL) occur on muddy offshore substrata (50–80 m deep) and at one year of age they recruit into the inshore zone. Adults are thought to predominantly occur inshore. Spawning occurs in the species during late spring. The species is heavily targeted by the recreational, subsistence, artisanal and commercial fisheries as juveniles through to adults and there are signs of population decline with declines in catch per unit effort (CPUE) and maximum size. A basic understanding of their movement ecology has emerged from previous studies, based on conventional tagging (mark-recapture) and CPUE monitoring from a shore-based recreational fishery. Juveniles were thought to be resident, with larger fish undertaking long distance migration southward in the austral summer and returning during the austral winter. In addition to the fishery-related threats faced by the species, the southern Angolan region has been identified to be an ocean warming hotspot, and this has been linked to a southward distribution shift and the recent hybridisation of A. coronus with its congener A. inodorus, in Namibia. This study aims to expand the knowledge of the movement ecology of A. coronus and to interrogate our current understanding of the movement patterns of the species using passive acoustic telemetry. Passive acoustic receivers were deployed at three study sites, Flamingo, where all tagging occurred, which lies ~ 200 km north of the Angolan border with Namibia, is a relatively exposed stretch of coastline; Tombua Bay, which lies 30 km south of Flamingo, is a small, sheltered natural embayment; and Baia dos Tigres, which lies 100 km south of Tombua Bay, is a sheltered lagoon in the lee of an island ~ 10 km from the mainland. Tagging occurred in two batches, one year apart. In the first batch, sub-adults (n = 3) and adults (n = 17) were tagged and monitored for two years. In the second batch, juveniles (n = 7) and sub-adults (n = 3) were tagged and monitored for one year. The first objective of this thesis (Chapter 3) was to examine the spatio-temporal dynamics of A. coronus at a regional-level (across study sites, to investigate the prevalence of the longshore migration), and at a local-level (within a study site), and to categorise the movement behaviour of juveniles, sub-adults and adults. Traditional seasons were not used in this thesis, as the study region alternates between ‘warm’ periods (up to ~ 26 °C mean daily water temperature), when Angola Current water covers the area, and ‘cold’ periods (down to ~ 15 °C mean daily water temperature), when Benguela Current water covers the area. A high degree of residency of tagged fish to the Flamingo study site, where tagging was conducted, was found, with juveniles and sub-adults never being detected outside of the Flamingo study site. Five adults (29 %) were detected haphazardly for between one and 36 days at Tombua Bay, following which they were typically detected again at the Flamingo study site. No fish were ever detected at the southernmost site, Baia dos Tigres, suggesting that none migrated to Namibia. Within the Flamingo study site, adults were found to group at the inshore mid-region of the study site during ‘cold’ periods, dispersing again during ‘warm’ periods, when there was a concomitant offshore shift in their area use. This explained the absence of the species from catches in the shore-based recreational fishery during ‘warm’ periods. These results challenge previous migration hypotheses for the species. Therefore, longshore return migrations which were evident from previously conducted conventional tagging, are probably not the norm for the species, but likely occur at a low frequency. The species exhibited a high degree of residency to their tagging site, despite the drastic seasonal changes in water temperatures and station-keeping was the dominant behaviour across all life stages. The constrained area-use noted in this study, relative to the known distribution of the species, suggests that A. coronus exists as a metapopulation, consisting of a network of subpopulations interconnected by gene flow that is most likely facilitated during their pelagic egg and larval phase. The second objective of this thesis was to examine the group formation observed at the Flamingo study site during ‘cold’ periods (Chapter 4). While this grouping of individuals was found to be correlated with water temperature, it was unlikely to be a causal relationship, as the water temperature was not dissimilar to adjacent areas. The area where individuals were concentrated is known to frequently attract large shoals of their dominant prey, Sardinella aurita. While the timing of this group formation aligns with a pre-spawning period, no studies have assessed the existence of local spawning. A. coronus were, however, likely utilising an abundant prey source, during the important pre-spawning period, in order to build up energy reserves. Group formation is a common feature of sciaenids, but literature on the subject is restricted to spawning congregations. Fish are not only more vulnerable to capture during these periods but may also be disturbed during these potentially important social periods. The third objective of this thesis was to investigate the presence of sociality in the species (Chapter 5). Evidence for sociality during and outside the seasonal group formation was explored using network analyses. There was evidence for sociality, with several groups, characterized by co-locations among individuals, identified. While these groups were fluid through time, there was evidence of persistent sociality, with two individuals in particular being consistently co-located over the entire study period. Due to the persistent nature of sociality, group foraging is suggested as an explanation for this, with anecdotal evidence of coordinated hunting supporting this. Acoustic telemetry vastly improved the knowledge of the movement ecology of A. coronus. Despite their panmictic population genetic structure, they were found to be largely resident, likely occurring as a metapopulation across their distribution, with egg and larval dispersal likely the primary mechanism for maintaining panmixia. Evidence for sociality was also found, which was previously unexplored in the species. The persistent nature of social groupings throughout the year, suggests that it may be linked with group foraging and is supported by anecdotal evidence of coordinated hunting by the species. While this study highlights the value of acoustic telemetry in studying movement ecology, it also shows the importance of drawing on multiple methods to fully understand a species’ spatial ecology, including mark-recapture, population genetics, and fishery catch and effort data. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
Spatial analysis of littoral and demersal fish assemblages within the Knysna Estuary system
- Authors: Meiklejohn, Andrew Keith
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424334 , vital:72144
- Description: The Knysna Estuary is a unique system as it is the only “estuarine bay” system in the warm-temperate region of South Africa and is the largest estuary system on the southern coastline of South Africa. The Knysna Estuary has been identified as the estuary with the highest conversation priority in South Africa. The volume of research undertaken on the Knysna Estuary has led to it being rated “excellent” in terms of research productivity. However, despite this, surprisingly little work has been undertaken and published around the dynamics of fish assemblages occurring in the system, with little to no research assessing the demersal fish assemblage. The last widespread fish sampling effort that has been published was conducted in 1994, highlighting the need for an updated fish assessment with a focused sampling effort targeting both the littoral and demersal fish populations. The identification of key habitats for estuarine fish assemblages is essential for addressing estuarine conservation needs. Despite the importance of spatial data in addressing conservation planning, few estuarine studies have used spatial analyses in Geographic Information Systems (GIS) to identify conservation priority areas. Such information is critical for effective estuarine management plans. The aim of this project was to identify juvenile fish density hotspots to inform future systematic conservation planning. This study made use of two methods of sampling, seine netting to target the littoral fish assemblage and beam trawling to target demersal fish species. Two dedicated sampling trips for each sampling method were undertaken in June 2021 and March 2022 for Seine net sampling and November 2021 and March 2022 for Beam trawl sampling. Fish were identified to species level, measured (mm TL) and categorised into life history stages (i.e. settlement stage, juvenile and adult) and thereafter assigned into their various estuarine association guilds. A total of 47 species were recorded, with 42 species were caught in the seine net sampling and 24 species in the beam trawl sampling. The results from this study showed the vast extent of marine dominance in the Knysna Estuary, with marine estuarine-opportunist (MEO) species dominating catches (richness) from both sampling methods. The spatial analysis highlighted the importance of the lower “marine bay” region of the system, with the majority of estuarine guilds showing a high abundance in this region. Key fishery species and dominant fish species were identified during this study, the dominant species during the sampling effort were identified as ecologically important fish species and were dominated by adult specimens. The key fishery species sampled during this study were dominated by juvenile specimens. This highlights the role of the Knysna Estuary as a nursery area for juvenile fishery species and the contribution of the estuary to the estuarine and adjacent coastal fisheries. Key hotspots were identified for fishery species, these being the “Ashmead Channel” in the marine bay region and the “Belvidere” section of the lagoon region. Ashmead channel is sheltered backwater area while the Belvidere section is some distance from the main river channel, reducing the anthropogenic impact on these areas. The low anthropogenic utilization of these areas along with weaker water current in these regions was linked to the usage of these region by key fishery species. These areas were highlighted as important conservation hotspots with both currently not adequately protected under the current habitat sensitivity management model. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Meiklejohn, Andrew Keith
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424334 , vital:72144
- Description: The Knysna Estuary is a unique system as it is the only “estuarine bay” system in the warm-temperate region of South Africa and is the largest estuary system on the southern coastline of South Africa. The Knysna Estuary has been identified as the estuary with the highest conversation priority in South Africa. The volume of research undertaken on the Knysna Estuary has led to it being rated “excellent” in terms of research productivity. However, despite this, surprisingly little work has been undertaken and published around the dynamics of fish assemblages occurring in the system, with little to no research assessing the demersal fish assemblage. The last widespread fish sampling effort that has been published was conducted in 1994, highlighting the need for an updated fish assessment with a focused sampling effort targeting both the littoral and demersal fish populations. The identification of key habitats for estuarine fish assemblages is essential for addressing estuarine conservation needs. Despite the importance of spatial data in addressing conservation planning, few estuarine studies have used spatial analyses in Geographic Information Systems (GIS) to identify conservation priority areas. Such information is critical for effective estuarine management plans. The aim of this project was to identify juvenile fish density hotspots to inform future systematic conservation planning. This study made use of two methods of sampling, seine netting to target the littoral fish assemblage and beam trawling to target demersal fish species. Two dedicated sampling trips for each sampling method were undertaken in June 2021 and March 2022 for Seine net sampling and November 2021 and March 2022 for Beam trawl sampling. Fish were identified to species level, measured (mm TL) and categorised into life history stages (i.e. settlement stage, juvenile and adult) and thereafter assigned into their various estuarine association guilds. A total of 47 species were recorded, with 42 species were caught in the seine net sampling and 24 species in the beam trawl sampling. The results from this study showed the vast extent of marine dominance in the Knysna Estuary, with marine estuarine-opportunist (MEO) species dominating catches (richness) from both sampling methods. The spatial analysis highlighted the importance of the lower “marine bay” region of the system, with the majority of estuarine guilds showing a high abundance in this region. Key fishery species and dominant fish species were identified during this study, the dominant species during the sampling effort were identified as ecologically important fish species and were dominated by adult specimens. The key fishery species sampled during this study were dominated by juvenile specimens. This highlights the role of the Knysna Estuary as a nursery area for juvenile fishery species and the contribution of the estuary to the estuarine and adjacent coastal fisheries. Key hotspots were identified for fishery species, these being the “Ashmead Channel” in the marine bay region and the “Belvidere” section of the lagoon region. Ashmead channel is sheltered backwater area while the Belvidere section is some distance from the main river channel, reducing the anthropogenic impact on these areas. The low anthropogenic utilization of these areas along with weaker water current in these regions was linked to the usage of these region by key fishery species. These areas were highlighted as important conservation hotspots with both currently not adequately protected under the current habitat sensitivity management model. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
Spatiotemporal ecology and potential displacement of white sharks in southern Africa
- Authors: Towner, Alison
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432275 , vital:72857
- Description: Restricted access. Expected release date in 2025. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Towner, Alison
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432275 , vital:72857
- Description: Restricted access. Expected release date in 2025. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
Species diversity and distribution patterns of three freshwater fish genera in southern Africa
- Authors: Mutizwa, Tadiwa Isaac
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431896 , vital:72813
- Description: Access restricted. Expected release in 2025. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Mutizwa, Tadiwa Isaac
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431896 , vital:72813
- Description: Access restricted. Expected release in 2025. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
The androgenic and anabolic effects of pine pollen on Nile tilapia (Oreochromis niloticus)
- Authors: Abaho, Ivan
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431615 , vital:72790
- Description: Access restricted. Expected release date in 2025 , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Abaho, Ivan
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431615 , vital:72790
- Description: Access restricted. Expected release date in 2025 , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
The effects of exploitation on the activity of Chrysoblephus laticeps in a thermally variable environment
- Authors: Mlotshwa, Nonhle Thubelihle
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424346 , vital:72145
- Description: Embargoed. Expected release date 2025. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Mlotshwa, Nonhle Thubelihle
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424346 , vital:72145
- Description: Embargoed. Expected release date 2025. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-10-13