An investigation into the pharmacological properties of propolis
- Authors: Hu, Fuliang
- Date: 2005
- Subjects: Propolis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5831 , http://hdl.handle.net/10962/d1007955
- Description: Propolis is a traditional value-added product from beekeeping, and has been widely used in medicine and the chemical industry because of its extensive biological activities. In this dissertation some pharmacological properties of propolis extracted by ethanol (EEP) and water (WSP) were studied by means of modem pharmacological methods. The results of the experiments show the following: 1. Both EEP and WSD led to decreased levels of fasting blood glucose (FBG), glycosylated haemoglobin (HBA1c), Fructosamine (FRU), blood urea nitrogen (BUN), uric acid (UA), malonaldehyde (MDA), nitric oxide (NO), nitric oxide synthetase (NOS), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and very-low density lipoprotein cholesterol (VLDL-C) in the serum of fasting rats; and, to increased serum levels of high-density lipoprotein cholesterol (HDL-C) and superoxide dismutase (SOD) in rats with diabetes mellitus. This suggests that propolis can control blood glucose and modulate the metabolism of glucose, blood lipid and protein, leading to decreased outputs of lipid peroxidation and scavenging free radicals in rats with diabetes mellitus. 2. Both EEP and WSD showed inhibitory effects on swelling induced by Freund's complete adjuvant and decreased the degree of local inflammatory responses; significantly inhibited the increase of interleukin-6 (lL-6) in inflamed tissues, but had no significant effect on levels of interleukin-2 (IL-2) and interferon-y (IFN-y). The results are consistent with the interpretation that EEP and WSD may exert these effects by inhibiting the activation and differentiation of mononuclear macrophages. 3. Both EEP and WSD exhibited significant anti-inflammatory effects in animal models with respect to thoracic capillary vessel leakage in mice, Carrageenan-induced oedema, Carrageen-aninduced pleurisy and acute lung damage in rats. The mechanisms for the anti-inflammatory effects probably involve decreasing prostaglandin and NO levels. 4. Both EEP and WSD had an inhibitory effect on the level of TG, TC, LDL-C, glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminase (GOT) in serum, and TC, TG and MDA in liver of hyperlipidemic SD rats; but were without effects on HDL-C, MDA, SOD and NO in serum. EEP also reduced body weight, liver weight and liver index, but WSD did not reduce those indexes. The results showed that the two extracts contribute to the improvement of lipid metabolism in hyperlipidemic rats and provide them with the required anti-oxidative activity. 5. The 80% ethanol extracts of propolis had the highest flavonoid content. The flavone content of water extracts of propolis were obtained with a natural surface-active agent, under ultrasonic perturbation at 80°C, for 12 h and was 6.7 times greater than that of propolis extracted with water at room temperature. Compared to EEP, WSD has the same or a greater anti-tumor or anti-inflammation effect at the same dosage, and shows considerable pharmacological potential especially because of its low side effects and ease of preparation.
- Full Text:
- Date Issued: 2005
- Authors: Hu, Fuliang
- Date: 2005
- Subjects: Propolis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5831 , http://hdl.handle.net/10962/d1007955
- Description: Propolis is a traditional value-added product from beekeeping, and has been widely used in medicine and the chemical industry because of its extensive biological activities. In this dissertation some pharmacological properties of propolis extracted by ethanol (EEP) and water (WSP) were studied by means of modem pharmacological methods. The results of the experiments show the following: 1. Both EEP and WSD led to decreased levels of fasting blood glucose (FBG), glycosylated haemoglobin (HBA1c), Fructosamine (FRU), blood urea nitrogen (BUN), uric acid (UA), malonaldehyde (MDA), nitric oxide (NO), nitric oxide synthetase (NOS), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and very-low density lipoprotein cholesterol (VLDL-C) in the serum of fasting rats; and, to increased serum levels of high-density lipoprotein cholesterol (HDL-C) and superoxide dismutase (SOD) in rats with diabetes mellitus. This suggests that propolis can control blood glucose and modulate the metabolism of glucose, blood lipid and protein, leading to decreased outputs of lipid peroxidation and scavenging free radicals in rats with diabetes mellitus. 2. Both EEP and WSD showed inhibitory effects on swelling induced by Freund's complete adjuvant and decreased the degree of local inflammatory responses; significantly inhibited the increase of interleukin-6 (lL-6) in inflamed tissues, but had no significant effect on levels of interleukin-2 (IL-2) and interferon-y (IFN-y). The results are consistent with the interpretation that EEP and WSD may exert these effects by inhibiting the activation and differentiation of mononuclear macrophages. 3. Both EEP and WSD exhibited significant anti-inflammatory effects in animal models with respect to thoracic capillary vessel leakage in mice, Carrageenan-induced oedema, Carrageen-aninduced pleurisy and acute lung damage in rats. The mechanisms for the anti-inflammatory effects probably involve decreasing prostaglandin and NO levels. 4. Both EEP and WSD had an inhibitory effect on the level of TG, TC, LDL-C, glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminase (GOT) in serum, and TC, TG and MDA in liver of hyperlipidemic SD rats; but were without effects on HDL-C, MDA, SOD and NO in serum. EEP also reduced body weight, liver weight and liver index, but WSD did not reduce those indexes. The results showed that the two extracts contribute to the improvement of lipid metabolism in hyperlipidemic rats and provide them with the required anti-oxidative activity. 5. The 80% ethanol extracts of propolis had the highest flavonoid content. The flavone content of water extracts of propolis were obtained with a natural surface-active agent, under ultrasonic perturbation at 80°C, for 12 h and was 6.7 times greater than that of propolis extracted with water at room temperature. Compared to EEP, WSD has the same or a greater anti-tumor or anti-inflammation effect at the same dosage, and shows considerable pharmacological potential especially because of its low side effects and ease of preparation.
- Full Text:
- Date Issued: 2005
Establishment and impact of the sap-sucking mirid, Falconia intermedia (Distant) (Hemiptera: Miridae) on Lantana camara (Verbenaceae) varieties in the Eastern Cape Province, South Africa
- Heshula, Unathi-Nkosi Lelethu Peter
- Authors: Heshula, Unathi-Nkosi Lelethu Peter
- Date: 2005
- Subjects: Lantana camara -- South Africa -- Eastern Cape , Biological pest control agents -- South Africa -- Eastern Cape , Hemiptera -- South Africa -- Eastern Cape , Miridae -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5832 , http://hdl.handle.net/10962/d1008085
- Description: The biological control of the weedy complex Lantana camara (L.) (Verbenaceae) has been ongoing in South Africa for over 40 years. Despite this, the weed is still not under sufficient control and continues to invade new territories in the country. The biological control programme needs to be bolstered with releases of new and potentially damaging biological control agents. A promising biological control agent endemic to Central America, Falconia intermedia (Distant) (Hemiptera: Miridae), was imported into quarantine from Jamaica in 1994. This agent was released on sites in KwaZulu-Natal and Limpopo provinces of South Africa in 1999. Even though it initially established and damaged L. camara, populations died out at most of the release sites. As varietal difference and adverse climate have been cited as the reason for non-establishment and ineffective control in L. camara biocontrol programmes worldwide, this study attempts to investigate the role that these two factors play in this weed herbivore relationship. Laboratory no-choice trials were conducted to determine the varietal performance of F intermedia, among five Eastern Cape varieties of the weed from East London, Whitney Farm, Heather Glen Farm, Port Alfred and Lyndhurst Farm, and a variety from the Plant Protection Research Institute (PPRI), Pretoria. However, there were differences in performance as the adult mirids performed better on white-pink varieties from Whitney Farm and Heather Glen Farm. To test varietal preference in field conditions, field releases of F intermedia were also made at East London, Whitney Farm, Heather Glen Farm, Port Alfred and Lyndhurst Farm. Post release evaluations were conducted monthly for two years (2002 and 2003). The insect established at East London and Whitney Farm, both of which have white-pink varieties. Insect populations quickly died out at the Lyndhurst Farm and Port Alfred sites, which have dark pink varieties. It is suggested that field conditions may have resulted in poor plant quality and led indirectly to varietal preference, and to non-establishment at these two sites. With the onset of cooler weather, populations disappeared at Heather Glen Farm. This suggested that F. intermedia was suitable for release in more subtropical areas within South Africa where climatic conditions would be suitable throughout the year. The mirid performed well at Whitney Farm, resulting in significant reduction in plant growth parameters such as height and percentage cover, and increasing the cover of other flora growing beneath L. camara plants. Finally, ways to improve the efficacy of this agent are considered in an effort towards better control of L. camara in South Africa.
- Full Text:
- Date Issued: 2005
- Authors: Heshula, Unathi-Nkosi Lelethu Peter
- Date: 2005
- Subjects: Lantana camara -- South Africa -- Eastern Cape , Biological pest control agents -- South Africa -- Eastern Cape , Hemiptera -- South Africa -- Eastern Cape , Miridae -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5832 , http://hdl.handle.net/10962/d1008085
- Description: The biological control of the weedy complex Lantana camara (L.) (Verbenaceae) has been ongoing in South Africa for over 40 years. Despite this, the weed is still not under sufficient control and continues to invade new territories in the country. The biological control programme needs to be bolstered with releases of new and potentially damaging biological control agents. A promising biological control agent endemic to Central America, Falconia intermedia (Distant) (Hemiptera: Miridae), was imported into quarantine from Jamaica in 1994. This agent was released on sites in KwaZulu-Natal and Limpopo provinces of South Africa in 1999. Even though it initially established and damaged L. camara, populations died out at most of the release sites. As varietal difference and adverse climate have been cited as the reason for non-establishment and ineffective control in L. camara biocontrol programmes worldwide, this study attempts to investigate the role that these two factors play in this weed herbivore relationship. Laboratory no-choice trials were conducted to determine the varietal performance of F intermedia, among five Eastern Cape varieties of the weed from East London, Whitney Farm, Heather Glen Farm, Port Alfred and Lyndhurst Farm, and a variety from the Plant Protection Research Institute (PPRI), Pretoria. However, there were differences in performance as the adult mirids performed better on white-pink varieties from Whitney Farm and Heather Glen Farm. To test varietal preference in field conditions, field releases of F intermedia were also made at East London, Whitney Farm, Heather Glen Farm, Port Alfred and Lyndhurst Farm. Post release evaluations were conducted monthly for two years (2002 and 2003). The insect established at East London and Whitney Farm, both of which have white-pink varieties. Insect populations quickly died out at the Lyndhurst Farm and Port Alfred sites, which have dark pink varieties. It is suggested that field conditions may have resulted in poor plant quality and led indirectly to varietal preference, and to non-establishment at these two sites. With the onset of cooler weather, populations disappeared at Heather Glen Farm. This suggested that F. intermedia was suitable for release in more subtropical areas within South Africa where climatic conditions would be suitable throughout the year. The mirid performed well at Whitney Farm, resulting in significant reduction in plant growth parameters such as height and percentage cover, and increasing the cover of other flora growing beneath L. camara plants. Finally, ways to improve the efficacy of this agent are considered in an effort towards better control of L. camara in South Africa.
- Full Text:
- Date Issued: 2005
Euphausiid population structure and grazing in the Indian sector of the Antarctic Polar Frontal Zone, during austral autumn
- Bernard, Anthony Thomas Firth
- Authors: Bernard, Anthony Thomas Firth
- Date: 2005
- Subjects: Euphausiacea -- Antarctic Ocean , Euphausiacea -- Feeding and feeds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5913 , http://hdl.handle.net/10962/d1015960
- Description: The trophodynamics of the numerically dominant euphausiid species within a region of high mesoscale oceanographic variability in the southwest Indian sector of the Antarctic Polar Frontal Zone (PFZ) were investigated during the austral autumns April/May) of 2004 and 2005. During the 2004 survey, sub-surface (200 m) temperature profiles indicated that an intense frontal feature, formed by the convergence of the Sub-Antarctic Front (SAF) and the Antarctic Polar Front (APF) bisected the survey area into two distinct zones, the Sub- Antarctic Zone (SAZ) and the Antarctic Zone (AAZ). Total integrated chlorophyll-a (chl-a) biomass was typical for the region (< 25 mg chl-a m⁻²), and was dominated by picophytoplankton. Total euphausiid abundance and biomass ranged from 0.1 to 3.1 ind m⁻³ and 0.1 to 8.1 mg dwt m⁻³, respectively, and did not differ significantly between the stations occupied in the SAZ and AAZ (p > 0.05). The multivariate analysis identified two interacting mechanisms controlling the distribution patterns, abundance and biomass of the various euphausiid species, namely (1) diel changes in abundance and biomass, (2) and restricted distribution patterns associated with the different water masses. Ingestion rates were determined for five euphausiid species. E. triacantha was found to have the highest daily ingestion rate ranging from 1 226.1 to 6 029.1 ng pigm ind⁻¹d⁻¹, while the lowest daily ingestion rates were observed in the juvenile Thysanoessa species (6.4 to 943.0 ng pigm ind⁻¹ d⁻¹). The total grazing impact of the selected euphausiids ranged from < 0.1 to 20.1 μg pigm m⁻²d⁻¹, corresponding to < 0.15 % of the areal chl-a biomass. The daily ration estimates of autotrophic carbon for the euphausiids suggested that phytoplankton represented a minor component in their diets, with only the sub-adult E. vallentini consuming sufficient phytoplankton to meet their daily carbon requirements. A cyclonic cold-core eddy spawned from the region of the APF located in the southwest Indian sector of the PFZ was the dominant feature during the 2005 survey. The total areal chl-a biomass throughout the region was low, ranging between 5.6 and 11.4 mg chl-a m⁻², and was significantly higher within the core of the eddy compared to the surrounding waters (p < 0.05). RMT-8 and WP-2 total euphausiid abundance and biomass estimates were high, and ranged from 0.004 to 0.36 ind m⁻³ and 0.065 to 1.21 mg dwt m⁻³, and from 0.01 to 18.2 ind m⁻³ and 0.01 to 15.7 mg dwt m⁻³, respectively. A distinct spatial pattern in the euphausiid community was evident with the Antarctic species, Euphausia frigida, E. triacantha and E. superba predominating within the core of the eddy, while the PFZ waters were characterized by the sub-Antarctic species, E. longirostris, Stylocheiron maximum, Nematoscelis megalops and Thysanoessa gregaria. The eddy edge acted as a transition zone where species from both regions co-occurred. Within the survey area the combined ingestion rate of the six numerically dominant euphausiid species ranged between 0.02 and 5.31 μg pigm m⁻²d¹, which corresponded to a loss of between < 0.001 and 0.11 % of the available chl-a biomass. E. triacantha and juvenile T. macura were identified as the dominant grazers. There was no apparent spatial pattern in the grazing activity of the euphausiids within the region of investigation. The average daily rations of the euphausiids examined were < 2 % of their body carbon. The low daily ration of the euphausiids could be ascribed to the predominance of small picophytoplankton in the region of investigation, which are too small to be grazed efficiently by larger zooplankton. The marked spatial patterns in species composition and the elevated abundance and biomass of euphausiids, suggest that the mesoscale eddies contribute to the spatial and temporal heterogeneity of the planktonic community of the PFZ and may represent important foraging regions for many of the apex predators within the region.
- Full Text:
- Date Issued: 2005
- Authors: Bernard, Anthony Thomas Firth
- Date: 2005
- Subjects: Euphausiacea -- Antarctic Ocean , Euphausiacea -- Feeding and feeds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5913 , http://hdl.handle.net/10962/d1015960
- Description: The trophodynamics of the numerically dominant euphausiid species within a region of high mesoscale oceanographic variability in the southwest Indian sector of the Antarctic Polar Frontal Zone (PFZ) were investigated during the austral autumns April/May) of 2004 and 2005. During the 2004 survey, sub-surface (200 m) temperature profiles indicated that an intense frontal feature, formed by the convergence of the Sub-Antarctic Front (SAF) and the Antarctic Polar Front (APF) bisected the survey area into two distinct zones, the Sub- Antarctic Zone (SAZ) and the Antarctic Zone (AAZ). Total integrated chlorophyll-a (chl-a) biomass was typical for the region (< 25 mg chl-a m⁻²), and was dominated by picophytoplankton. Total euphausiid abundance and biomass ranged from 0.1 to 3.1 ind m⁻³ and 0.1 to 8.1 mg dwt m⁻³, respectively, and did not differ significantly between the stations occupied in the SAZ and AAZ (p > 0.05). The multivariate analysis identified two interacting mechanisms controlling the distribution patterns, abundance and biomass of the various euphausiid species, namely (1) diel changes in abundance and biomass, (2) and restricted distribution patterns associated with the different water masses. Ingestion rates were determined for five euphausiid species. E. triacantha was found to have the highest daily ingestion rate ranging from 1 226.1 to 6 029.1 ng pigm ind⁻¹d⁻¹, while the lowest daily ingestion rates were observed in the juvenile Thysanoessa species (6.4 to 943.0 ng pigm ind⁻¹ d⁻¹). The total grazing impact of the selected euphausiids ranged from < 0.1 to 20.1 μg pigm m⁻²d⁻¹, corresponding to < 0.15 % of the areal chl-a biomass. The daily ration estimates of autotrophic carbon for the euphausiids suggested that phytoplankton represented a minor component in their diets, with only the sub-adult E. vallentini consuming sufficient phytoplankton to meet their daily carbon requirements. A cyclonic cold-core eddy spawned from the region of the APF located in the southwest Indian sector of the PFZ was the dominant feature during the 2005 survey. The total areal chl-a biomass throughout the region was low, ranging between 5.6 and 11.4 mg chl-a m⁻², and was significantly higher within the core of the eddy compared to the surrounding waters (p < 0.05). RMT-8 and WP-2 total euphausiid abundance and biomass estimates were high, and ranged from 0.004 to 0.36 ind m⁻³ and 0.065 to 1.21 mg dwt m⁻³, and from 0.01 to 18.2 ind m⁻³ and 0.01 to 15.7 mg dwt m⁻³, respectively. A distinct spatial pattern in the euphausiid community was evident with the Antarctic species, Euphausia frigida, E. triacantha and E. superba predominating within the core of the eddy, while the PFZ waters were characterized by the sub-Antarctic species, E. longirostris, Stylocheiron maximum, Nematoscelis megalops and Thysanoessa gregaria. The eddy edge acted as a transition zone where species from both regions co-occurred. Within the survey area the combined ingestion rate of the six numerically dominant euphausiid species ranged between 0.02 and 5.31 μg pigm m⁻²d¹, which corresponded to a loss of between < 0.001 and 0.11 % of the available chl-a biomass. E. triacantha and juvenile T. macura were identified as the dominant grazers. There was no apparent spatial pattern in the grazing activity of the euphausiids within the region of investigation. The average daily rations of the euphausiids examined were < 2 % of their body carbon. The low daily ration of the euphausiids could be ascribed to the predominance of small picophytoplankton in the region of investigation, which are too small to be grazed efficiently by larger zooplankton. The marked spatial patterns in species composition and the elevated abundance and biomass of euphausiids, suggest that the mesoscale eddies contribute to the spatial and temporal heterogeneity of the planktonic community of the PFZ and may represent important foraging regions for many of the apex predators within the region.
- Full Text:
- Date Issued: 2005
Growth, feeding and sex change in the sequential protandric shrimp Nauticaris marionis Bate 1888 at the Prince Edward Islands (Southern Ocean)
- Authors: Vumazonke, Lukhanyiso Unam
- Date: 2005
- Subjects: Shrimps -- Prince Edward Islands , Shrimps -- Feeding and feeds , Shrimps -- Nutrition , Shrimps -- Growth
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5704 , http://hdl.handle.net/10962/d1005390 , Shrimps -- Prince Edward Islands , Shrimps -- Feeding and feeds , Shrimps -- Nutrition , Shrimps -- Growth
- Description: Demographic parameters and the general biology of the subantarctic shrimp Nauticaris marionis from the Prince Edward Islands were investigated. The carapace length is the most accurate indicator of body size and it was confirmed that N. marionis is a partially protandric hermaphrodite. Gravid females of N. marionis observed mainly in March with negligible hatching persisting until April/May. The majority of juveniles develop into males. Juveniles are characterised by an appendage on the endopodite of the first pleopod called the appendix interna or a.i.1. Juveniles that develop into males do so by growing a further appendage on the endopodite of the second pleopod. This appendage characterises males and is called the appendix masculina (or a.m.). Juveniles may develop directly into primary females through the development of an ovary and the loss of the a.i.1. The sequence differs among individuals, with some developing the ovary before losing the a.i.1 and others losing the a.i.1 first. Loss of the a.i.1 appears to be by shedding during a single moult, rather than by atrophy. Females can also develop by an alternative route as secondary females. Such animals first become males with an a.m. and then develop an ovary, thus forming an intermediate form called a tertium quid, or “third thing”. Again, there are two forms of tertium quid. Tertia quae a have the a.m. as well as the a.i.1. Tertia quae b have the a.m. in combination with an ovary but no a.i.1. Either form of tertium quid can develop into a secondary female through loss of the a.m., and in the case of the tertium quid a, loss of the a.i.1. It is unclear whether sexual differentiation occurs in the plankton or just after N. marionis settles on the benthos. The results of gut content analysis suggest that N. marionis is an opportunistic feeder, preying on a variety of prey with a preference for detritus, benthic amphipods and gastropods. Cannibalism of conspecific pleopods by large individuals of N. marionis occurred mostly in females, particularly in incubation containers. Cannibalism also occurs in males, and its occurrence depends on individual size, not gender. No diel patterns were observed in the feeding activity of N. marionis. In situ daily rations of males (carapace length <7mm) and females (>7mm) were equivalent to ≈10% and ≈5% of body dry weight, respectively. The von Bertalanffy growth curve parameters were empirically identified, by cohort analysis of data collected during 4 years, as K = 0.22239/year, L[subscript]∞ = 14.05789mm, t₀ = -0.05174, L₀ = 0.16083mm. N. marionis can survive up to seven years under natural conditions.
- Full Text:
- Date Issued: 2005
- Authors: Vumazonke, Lukhanyiso Unam
- Date: 2005
- Subjects: Shrimps -- Prince Edward Islands , Shrimps -- Feeding and feeds , Shrimps -- Nutrition , Shrimps -- Growth
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5704 , http://hdl.handle.net/10962/d1005390 , Shrimps -- Prince Edward Islands , Shrimps -- Feeding and feeds , Shrimps -- Nutrition , Shrimps -- Growth
- Description: Demographic parameters and the general biology of the subantarctic shrimp Nauticaris marionis from the Prince Edward Islands were investigated. The carapace length is the most accurate indicator of body size and it was confirmed that N. marionis is a partially protandric hermaphrodite. Gravid females of N. marionis observed mainly in March with negligible hatching persisting until April/May. The majority of juveniles develop into males. Juveniles are characterised by an appendage on the endopodite of the first pleopod called the appendix interna or a.i.1. Juveniles that develop into males do so by growing a further appendage on the endopodite of the second pleopod. This appendage characterises males and is called the appendix masculina (or a.m.). Juveniles may develop directly into primary females through the development of an ovary and the loss of the a.i.1. The sequence differs among individuals, with some developing the ovary before losing the a.i.1 and others losing the a.i.1 first. Loss of the a.i.1 appears to be by shedding during a single moult, rather than by atrophy. Females can also develop by an alternative route as secondary females. Such animals first become males with an a.m. and then develop an ovary, thus forming an intermediate form called a tertium quid, or “third thing”. Again, there are two forms of tertium quid. Tertia quae a have the a.m. as well as the a.i.1. Tertia quae b have the a.m. in combination with an ovary but no a.i.1. Either form of tertium quid can develop into a secondary female through loss of the a.m., and in the case of the tertium quid a, loss of the a.i.1. It is unclear whether sexual differentiation occurs in the plankton or just after N. marionis settles on the benthos. The results of gut content analysis suggest that N. marionis is an opportunistic feeder, preying on a variety of prey with a preference for detritus, benthic amphipods and gastropods. Cannibalism of conspecific pleopods by large individuals of N. marionis occurred mostly in females, particularly in incubation containers. Cannibalism also occurs in males, and its occurrence depends on individual size, not gender. No diel patterns were observed in the feeding activity of N. marionis. In situ daily rations of males (carapace length <7mm) and females (>7mm) were equivalent to ≈10% and ≈5% of body dry weight, respectively. The von Bertalanffy growth curve parameters were empirically identified, by cohort analysis of data collected during 4 years, as K = 0.22239/year, L[subscript]∞ = 14.05789mm, t₀ = -0.05174, L₀ = 0.16083mm. N. marionis can survive up to seven years under natural conditions.
- Full Text:
- Date Issued: 2005
Impacts of wildlife and cattle grazing on spider (araneae) biodiversity in a highland savanna ecosystem, in Laikipia, Central Kenya
- Authors: Warui, Charles Mwaura
- Date: 2005
- Subjects: Spiders -- Kenya Environmental monitoring -- Kenya Savanna ecology -- Kenya Biodiversity -- Kenya Species diversity -- Kenya Wildlife management -- Kenya Herbivores -- Ecology -- Kenya
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5712 , http://hdl.handle.net/10962/d1005398
- Description: Spiders were sampled at Mpala Research Centre, Laikipia, Kenya by pitfall-trapping and sweep-netting from May 2001 to July 2002, at a Kenyan Long-term Exclosure Experiment. The aim was to establish species composition, checklist and examine spider responses to disturbances caused by cattle, megaherbivores (giraffe and elephants) and mesoherbivores (other ungulates) by looking at three levels of resolution, namely the overall community, guilds and individual species. This is the first controlled replicated experimental study on the effects on invertebrates (spiders) by different land uses (access by large herbivores). A total of 10,487 individuals from 132 species belonging to 30 families were recorded. The family Salticidae had the highest number of species (24), followed by Gnaphosidae (20), Araneidae and Lycosidae (15 each), Theridiidae and Thomisidae (8 each) and Zodariidae (4). Most of the other families had fewer than 4 species. Throughout the study period, species not previously sampled emerged after rainfall peaks. Exclosure treatments affected plant cover, spider diversity and total species mainly through the effects of cattle, whose presence significantly reduced relative vegetation cover. An increase in vegetation cover significantly increased the diversity, total species and species evenness of the overall spider community (total samples data set). Megaherbivores and mesoherbivores had no effects on overall spider diversity. Relative vegetation cover explained approximately 20-30% of variation in community diversity, species richness and species evenness. At the guild level of resolution, the exclosure treatments had no significant effects on diversity, species richness and species evenness of web builders, plant wanderers and ground wanderers. Plant wanderers were significantly and positively correlated with relative vegetation cover, which explained 17% of variation in their diversity. Six individual species responded strongly and in contrasting ways to the same environmental variables, indicating that this level was more sensitive to environmental changes than guilds or the overall spider community. Spider diversity, relative vegetation cover and rainfall varied at a temporal scale of months and not at a spatial scale of hundreds of metres. Only species diversity and species richness from sweep-netting samples and total species from pitfall-trapping varied significantly at a spatial scale of hundreds of metres. Ordination analysis revealed that sweep-netting samples were a better indicator of grazing impacts than pitfalltrapping or combined samples and grouped to reflect cattle grazing, non-cattle grazing and to a small extent the control treatments. Other ordination analyses showed that only samples from sweep-netting and not from pitfall-trapping, were spatially partitioned at a scale of hundreds of metres. This study concludes that the spider fauna of black cotton soil habitats is rich and useful for environmental monitoring and that monitoring of several individual species as indicator of grazing impacts in savanna could be useful and relatively easy.
- Full Text:
- Date Issued: 2005
- Authors: Warui, Charles Mwaura
- Date: 2005
- Subjects: Spiders -- Kenya Environmental monitoring -- Kenya Savanna ecology -- Kenya Biodiversity -- Kenya Species diversity -- Kenya Wildlife management -- Kenya Herbivores -- Ecology -- Kenya
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5712 , http://hdl.handle.net/10962/d1005398
- Description: Spiders were sampled at Mpala Research Centre, Laikipia, Kenya by pitfall-trapping and sweep-netting from May 2001 to July 2002, at a Kenyan Long-term Exclosure Experiment. The aim was to establish species composition, checklist and examine spider responses to disturbances caused by cattle, megaherbivores (giraffe and elephants) and mesoherbivores (other ungulates) by looking at three levels of resolution, namely the overall community, guilds and individual species. This is the first controlled replicated experimental study on the effects on invertebrates (spiders) by different land uses (access by large herbivores). A total of 10,487 individuals from 132 species belonging to 30 families were recorded. The family Salticidae had the highest number of species (24), followed by Gnaphosidae (20), Araneidae and Lycosidae (15 each), Theridiidae and Thomisidae (8 each) and Zodariidae (4). Most of the other families had fewer than 4 species. Throughout the study period, species not previously sampled emerged after rainfall peaks. Exclosure treatments affected plant cover, spider diversity and total species mainly through the effects of cattle, whose presence significantly reduced relative vegetation cover. An increase in vegetation cover significantly increased the diversity, total species and species evenness of the overall spider community (total samples data set). Megaherbivores and mesoherbivores had no effects on overall spider diversity. Relative vegetation cover explained approximately 20-30% of variation in community diversity, species richness and species evenness. At the guild level of resolution, the exclosure treatments had no significant effects on diversity, species richness and species evenness of web builders, plant wanderers and ground wanderers. Plant wanderers were significantly and positively correlated with relative vegetation cover, which explained 17% of variation in their diversity. Six individual species responded strongly and in contrasting ways to the same environmental variables, indicating that this level was more sensitive to environmental changes than guilds or the overall spider community. Spider diversity, relative vegetation cover and rainfall varied at a temporal scale of months and not at a spatial scale of hundreds of metres. Only species diversity and species richness from sweep-netting samples and total species from pitfall-trapping varied significantly at a spatial scale of hundreds of metres. Ordination analysis revealed that sweep-netting samples were a better indicator of grazing impacts than pitfalltrapping or combined samples and grouped to reflect cattle grazing, non-cattle grazing and to a small extent the control treatments. Other ordination analyses showed that only samples from sweep-netting and not from pitfall-trapping, were spatially partitioned at a scale of hundreds of metres. This study concludes that the spider fauna of black cotton soil habitats is rich and useful for environmental monitoring and that monitoring of several individual species as indicator of grazing impacts in savanna could be useful and relatively easy.
- Full Text:
- Date Issued: 2005
Population dynamics of selected ichthyofaunal components in the temperate, temporarily open/closed Kasouga Estuary, South Africa
- Authors: Tweddle, Gavin Paul
- Date: 2005
- Subjects: Estuaries -- South Africa -- Eastern Cape , Estuarine fishes -- South Africa -- Eastern Cape , Estuarine ecology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5742 , http://hdl.handle.net/10962/d1005428 , Estuaries -- South Africa -- Eastern Cape , Estuarine fishes -- South Africa -- Eastern Cape , Estuarine ecology -- South Africa -- Eastern Cape
- Description: The spatial and temporal pattern of ichthyofaunal community composition in relation to selected physico-chemical (temperature and salinity) and biological variables (chlorophyll-a and zooplankton) was investigated at ten stations in the temperate temporarily open/closed Kasouga estuary. In addition, the food web structure in the estuary was investigated using stable carbon isotope analysis. Results of the 5 metre seine net survey indicated that ichthyofaunal composition and biomass in the Kasouga estuary was largely determined by seasonality and mouth condition. Maximum abundance and biomass of ichthyofauna was recorded during summer or during those periods when overtopping occurred. Overtopping coincided with the recruitment of marine estuarine dependant species, which dominated the catches both numerically and in biomass. The recruitment of these species resulted in an increase in diversity of the ichthyofaunal community. There were no significant spatial patterns in the distribution of smaller ichthyofauna (<50mm SL) identified in Bray-Curtis similarity matrices using cluster analysis (Primer 5 v5.2.4). Spearman’s correlation analysis revealed that there were no significant correlations between abundance and biomass of ichthyofauna and selected physico-chemical and biological variables other than salinity (P>0.05 in all other cases). There were two distinct spatial patterns in the distribution of the larger ichthyofauna (>50mm SL). These corresponded to a grouping associated with the mouth region and a grouping associated with the remaining regions of the estuary. Stable isotope analysis indicated that the primary source of carbon utilised by the ichthyofauna of the Kasouga estuary was derived from the channel, most likely microphytobenthic algae. The contributions of the riparian and salt marsh vegetation to the total carbon flow appear to be minimal.
- Full Text:
- Date Issued: 2005
- Authors: Tweddle, Gavin Paul
- Date: 2005
- Subjects: Estuaries -- South Africa -- Eastern Cape , Estuarine fishes -- South Africa -- Eastern Cape , Estuarine ecology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5742 , http://hdl.handle.net/10962/d1005428 , Estuaries -- South Africa -- Eastern Cape , Estuarine fishes -- South Africa -- Eastern Cape , Estuarine ecology -- South Africa -- Eastern Cape
- Description: The spatial and temporal pattern of ichthyofaunal community composition in relation to selected physico-chemical (temperature and salinity) and biological variables (chlorophyll-a and zooplankton) was investigated at ten stations in the temperate temporarily open/closed Kasouga estuary. In addition, the food web structure in the estuary was investigated using stable carbon isotope analysis. Results of the 5 metre seine net survey indicated that ichthyofaunal composition and biomass in the Kasouga estuary was largely determined by seasonality and mouth condition. Maximum abundance and biomass of ichthyofauna was recorded during summer or during those periods when overtopping occurred. Overtopping coincided with the recruitment of marine estuarine dependant species, which dominated the catches both numerically and in biomass. The recruitment of these species resulted in an increase in diversity of the ichthyofaunal community. There were no significant spatial patterns in the distribution of smaller ichthyofauna (<50mm SL) identified in Bray-Curtis similarity matrices using cluster analysis (Primer 5 v5.2.4). Spearman’s correlation analysis revealed that there were no significant correlations between abundance and biomass of ichthyofauna and selected physico-chemical and biological variables other than salinity (P>0.05 in all other cases). There were two distinct spatial patterns in the distribution of the larger ichthyofauna (>50mm SL). These corresponded to a grouping associated with the mouth region and a grouping associated with the remaining regions of the estuary. Stable isotope analysis indicated that the primary source of carbon utilised by the ichthyofauna of the Kasouga estuary was derived from the channel, most likely microphytobenthic algae. The contributions of the riparian and salt marsh vegetation to the total carbon flow appear to be minimal.
- Full Text:
- Date Issued: 2005
Population structure, physiology and feeding ecology of the mysid Mesopodopsis wooldridgei (Whittmann) in a large permanently open estuary
- Authors: Mesher, Colette Suzanne
- Date: 2005
- Subjects: Mysidacea -- Physiology , Mysidacea -- Ecology , Mysidacea -- Feeding and feeds , Zooplankton -- South Africa -- Great Fish Estuary
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5746 , http://hdl.handle.net/10962/d1005432 , Mysidacea -- Physiology , Mysidacea -- Ecology , Mysidacea -- Feeding and feeds , Zooplankton -- South Africa -- Great Fish Estuary
- Description: This project had three main aims. The first aim was to investigate the metabolic physiology of the mysid Mesopodopsis wooldridgei. Secondly, the study examined the contribution of the mysid to the zooplankton community. Finally the study attempted to clarify the primary sources of carbon utilised by the mysid using stable carbon isotope analysis. The study was conducted in the freshwater dominated permanently open Great Fish Estuary (33°30’S; 27°08’E) located along the south-eastern coastline of southern Africa. Mass specific oxygen consumption rates of Mesopodopsis wooldridgei ranged between 0.11 and 8.38 ml O2 mg ww-1 h-1 and increased with an increase in water temperature. There were no significant differences in the respiration rates between the male and female mysids (P > 0.05). At a constant temperature (20°C), the oxygen consumption rate of M. wooldridgei decreased with an increase in salinity. Salinity tolerance of the mysid was strongly modified by temperature. At 5‰ and 30°C, mysids exhibited mass mortality. The Q10 value for M. wooldridgei at 15, 25 and 35‰ was estimated at 2.34, 1.44 and 2.14, respectively. Results of the study suggest that M. wooldridgei is well adapted to surviving in environments characterised by variations in temperature and salinity. Within the Great Fish Estuary, total chl. a concentration ranged between 2.68 μg L-1 and 31.12 μg L-1 and was always dominated by large phytoplankton cells (>5 μm). Average zooplankton abundance ranged between 62 and 28 917 ind. m-3 and biomass between 10 and 203 mg Dwt m-3. The zooplankton community was numerically dominated by the calanoid copepod Pseudodiaptomus hessei, which comprised up to 100% (range between 12 and 100%) of the total zooplankton counted. Total zooplankton biomass during the day was dominated by copepods and by mysids during the nighttime. Among the mysids, M. wooldridgei was the most numerically abundant mysid and comprised <10% of the total zooplankton abundance. Numerical analysis found no seasonal pattern in the total zooplankton abundance (including mysids). The lack of any seasonality could be attributed to the continuous freshwater inflow into the estuary due to the inter-basin transfer of water from the Gariep Dam to the Fish River system. A distinct spatial pattern in the zooplankton community structure was evident with the upper stations almost entirely dominated by the copepod P. hessei, while at stations occupied in the lower reaches of the estuary, the zooplankton community comprised a mixture of freshwater, estuarine and marine breeding zooplankton species. The mysids also demonstrated a distinct spatial pattern in their distribution. Mysids were generally absent from the upper reaches, while in the middle reaches Rhopalophthalmus terranatalis and M. wooldridgei were numerically dominant. Gastrosaccus brevifissura dominated in the lower regions of the estuary. Stable isotope analysis (δ13C) indicated that the dominant source of carbon utilised by the numerically dominant copepods and juvenile M. wooldridgei within the estuary was derived from the extensive phytoplankton stocks within the system. In contrast, sub-adult and adult mysids (R. terranatalis, M. wooldridgei and G. brevifissura) appeared to consume a combination of phytoplankton and copepods. The contribution of the various sources of carbon to the total carbon intake of the mysid remains unknown.
- Full Text:
- Date Issued: 2005
- Authors: Mesher, Colette Suzanne
- Date: 2005
- Subjects: Mysidacea -- Physiology , Mysidacea -- Ecology , Mysidacea -- Feeding and feeds , Zooplankton -- South Africa -- Great Fish Estuary
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5746 , http://hdl.handle.net/10962/d1005432 , Mysidacea -- Physiology , Mysidacea -- Ecology , Mysidacea -- Feeding and feeds , Zooplankton -- South Africa -- Great Fish Estuary
- Description: This project had three main aims. The first aim was to investigate the metabolic physiology of the mysid Mesopodopsis wooldridgei. Secondly, the study examined the contribution of the mysid to the zooplankton community. Finally the study attempted to clarify the primary sources of carbon utilised by the mysid using stable carbon isotope analysis. The study was conducted in the freshwater dominated permanently open Great Fish Estuary (33°30’S; 27°08’E) located along the south-eastern coastline of southern Africa. Mass specific oxygen consumption rates of Mesopodopsis wooldridgei ranged between 0.11 and 8.38 ml O2 mg ww-1 h-1 and increased with an increase in water temperature. There were no significant differences in the respiration rates between the male and female mysids (P > 0.05). At a constant temperature (20°C), the oxygen consumption rate of M. wooldridgei decreased with an increase in salinity. Salinity tolerance of the mysid was strongly modified by temperature. At 5‰ and 30°C, mysids exhibited mass mortality. The Q10 value for M. wooldridgei at 15, 25 and 35‰ was estimated at 2.34, 1.44 and 2.14, respectively. Results of the study suggest that M. wooldridgei is well adapted to surviving in environments characterised by variations in temperature and salinity. Within the Great Fish Estuary, total chl. a concentration ranged between 2.68 μg L-1 and 31.12 μg L-1 and was always dominated by large phytoplankton cells (>5 μm). Average zooplankton abundance ranged between 62 and 28 917 ind. m-3 and biomass between 10 and 203 mg Dwt m-3. The zooplankton community was numerically dominated by the calanoid copepod Pseudodiaptomus hessei, which comprised up to 100% (range between 12 and 100%) of the total zooplankton counted. Total zooplankton biomass during the day was dominated by copepods and by mysids during the nighttime. Among the mysids, M. wooldridgei was the most numerically abundant mysid and comprised <10% of the total zooplankton abundance. Numerical analysis found no seasonal pattern in the total zooplankton abundance (including mysids). The lack of any seasonality could be attributed to the continuous freshwater inflow into the estuary due to the inter-basin transfer of water from the Gariep Dam to the Fish River system. A distinct spatial pattern in the zooplankton community structure was evident with the upper stations almost entirely dominated by the copepod P. hessei, while at stations occupied in the lower reaches of the estuary, the zooplankton community comprised a mixture of freshwater, estuarine and marine breeding zooplankton species. The mysids also demonstrated a distinct spatial pattern in their distribution. Mysids were generally absent from the upper reaches, while in the middle reaches Rhopalophthalmus terranatalis and M. wooldridgei were numerically dominant. Gastrosaccus brevifissura dominated in the lower regions of the estuary. Stable isotope analysis (δ13C) indicated that the dominant source of carbon utilised by the numerically dominant copepods and juvenile M. wooldridgei within the estuary was derived from the extensive phytoplankton stocks within the system. In contrast, sub-adult and adult mysids (R. terranatalis, M. wooldridgei and G. brevifissura) appeared to consume a combination of phytoplankton and copepods. The contribution of the various sources of carbon to the total carbon intake of the mysid remains unknown.
- Full Text:
- Date Issued: 2005
The biology of suburban olive thrushes (Turdus olivaceus olivaceus) in the Eastern Cape, South Africa
- Authors: Bonnevie, Bo Tørris
- Date: 2005
- Subjects: Thrushes , Turdus , Thrushes -- South Africa -- Eastern Cape , Turdus -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5651 , http://hdl.handle.net/10962/d1005333 , Thrushes , Turdus , Thrushes -- South Africa -- Eastern Cape , Turdus -- South Africa -- Eastern Cape
- Description: This study investigated the biology of the Olive Thrush Turdus olivaceus olivaceus in Grahamstown, South Africa from 1998 to 2003. Behavioural differences between males and females, parent-young interactions, development of fledglings, and the role of song and moult in territorial behaviour were investigated. Comparisons of biometrics, moult and survival were made using Olive Thrush ringing data from other regions. There were no significant differences in mass, wing length or survival rate between the Eastern and Western Cape provinces, but primary wing moult started earlier in the Western Cape and coincided with the end of the main breeding periods in both provinces. Both males and females of breeding pairs remained in and defended their territories throughout the year, but there was some evidence that territorial defence was strongest during the breeding periods. Roughly, every 100 eggs laid produce 50 fledglings. Out of these 20 to 30 reach the age of independence at approximately 50 days, and only five of these juvenile birds reach maturity. Adult survival was estimated at 80%, with a mean life expectancy of 4.5 years and a conservative estimate of maximum lifespan of 11 years. Using ringing data and museum specimens, the Olive Thrush was compared with the Karoo Thrush Turdus smithi, a former race of the Olive Thrush. Olive Thrushes had shorter bills and wings, but were heavier than Karoo Thrushes from the Cradock district. There were also differences in bill and eye-ring colouration between these populations. No morphological differences were found between the sexes in either species.
- Full Text:
- Date Issued: 2005
- Authors: Bonnevie, Bo Tørris
- Date: 2005
- Subjects: Thrushes , Turdus , Thrushes -- South Africa -- Eastern Cape , Turdus -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5651 , http://hdl.handle.net/10962/d1005333 , Thrushes , Turdus , Thrushes -- South Africa -- Eastern Cape , Turdus -- South Africa -- Eastern Cape
- Description: This study investigated the biology of the Olive Thrush Turdus olivaceus olivaceus in Grahamstown, South Africa from 1998 to 2003. Behavioural differences between males and females, parent-young interactions, development of fledglings, and the role of song and moult in territorial behaviour were investigated. Comparisons of biometrics, moult and survival were made using Olive Thrush ringing data from other regions. There were no significant differences in mass, wing length or survival rate between the Eastern and Western Cape provinces, but primary wing moult started earlier in the Western Cape and coincided with the end of the main breeding periods in both provinces. Both males and females of breeding pairs remained in and defended their territories throughout the year, but there was some evidence that territorial defence was strongest during the breeding periods. Roughly, every 100 eggs laid produce 50 fledglings. Out of these 20 to 30 reach the age of independence at approximately 50 days, and only five of these juvenile birds reach maturity. Adult survival was estimated at 80%, with a mean life expectancy of 4.5 years and a conservative estimate of maximum lifespan of 11 years. Using ringing data and museum specimens, the Olive Thrush was compared with the Karoo Thrush Turdus smithi, a former race of the Olive Thrush. Olive Thrushes had shorter bills and wings, but were heavier than Karoo Thrushes from the Cradock district. There were also differences in bill and eye-ring colouration between these populations. No morphological differences were found between the sexes in either species.
- Full Text:
- Date Issued: 2005
The effects of the invasive mussel mytilus galloprovincialis and human exploitation on the indigenous mussel Perna perna on the South Coast of South Africa
- Authors: Rius Viladomiu, Marc
- Date: 2005
- Subjects: Mussels -- South Africa , Perna -- South Africa , Mytilus galloprovincialis -- South Africa , Mytilidae -- South Africa , Biological invasions , Marine resources conservation -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5684 , http://hdl.handle.net/10962/d1005370 , Mussels -- South Africa , Perna -- South Africa , Mytilus galloprovincialis -- South Africa , Mytilidae -- South Africa , Biological invasions , Marine resources conservation -- South Africa
- Description: In South Africa, the indigenous mussel Perna perna is threatened by both an invasive species and excessive human exploitation. The Mediterranean mussel Mytilus galloprovincialis is an invasive species that has been introduced to many parts of the world. In South Africa, this species arrived in the 1970s and spread rapidly along the west coast where today it is the dominant mussel species. Along the west coast, M. galloprovincialis is competitively superior in all aspects to the indigenous mussel species, and, as a result, has displaced some of them. On the south coast, M. galloprovincialis found more oligotrophic waters, higher species richness, and a stronger competitor in the indigenous mussel P. perna. The rate of spread of M. galloprovincialis along the south coast has decreased over the last 10 years and the present eastern limit of its distribution in South African is East London. On the south coast, M. galloprovincialis has not yet completely replaced P. perna; instead, the two exhibit spatial segregation, with P. perna dominating the low shore, M. galloprovincialis the high shore and an overlap zone between the two. An experiment on competition was carried out at one site on the south coast. The results showed that, on the low shore, P. perna is a more dominant competitor for space than M. galloprovincialis. Also byssus attachment of the two species differs, P. perna being much stronger than M. galloprovincialis, which suffers high mortality due to wave action on the low shore, especially in monospecific beds. As a result, mortality of M. galloprovincialis through wave action is reduced by the presence of P. perna, which seems to confer protection against dislodgement. However, in the absence of strong wave action, P. perna competitively excludes M. galloprovincialis. Human exploitation along 160 km of coast was examined by sampling mussel populations and using aerial surveys to determine where harvesters were distributed. Collectors did not seem to discriminate between species. The study has shown that higher abundances of mussels were found in protected or inaccessible sites, while in unprotected sites mussels were scarce. Coastal nature reserves are being proven to be effective in protecting mussel populations.
- Full Text:
- Date Issued: 2005
- Authors: Rius Viladomiu, Marc
- Date: 2005
- Subjects: Mussels -- South Africa , Perna -- South Africa , Mytilus galloprovincialis -- South Africa , Mytilidae -- South Africa , Biological invasions , Marine resources conservation -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5684 , http://hdl.handle.net/10962/d1005370 , Mussels -- South Africa , Perna -- South Africa , Mytilus galloprovincialis -- South Africa , Mytilidae -- South Africa , Biological invasions , Marine resources conservation -- South Africa
- Description: In South Africa, the indigenous mussel Perna perna is threatened by both an invasive species and excessive human exploitation. The Mediterranean mussel Mytilus galloprovincialis is an invasive species that has been introduced to many parts of the world. In South Africa, this species arrived in the 1970s and spread rapidly along the west coast where today it is the dominant mussel species. Along the west coast, M. galloprovincialis is competitively superior in all aspects to the indigenous mussel species, and, as a result, has displaced some of them. On the south coast, M. galloprovincialis found more oligotrophic waters, higher species richness, and a stronger competitor in the indigenous mussel P. perna. The rate of spread of M. galloprovincialis along the south coast has decreased over the last 10 years and the present eastern limit of its distribution in South African is East London. On the south coast, M. galloprovincialis has not yet completely replaced P. perna; instead, the two exhibit spatial segregation, with P. perna dominating the low shore, M. galloprovincialis the high shore and an overlap zone between the two. An experiment on competition was carried out at one site on the south coast. The results showed that, on the low shore, P. perna is a more dominant competitor for space than M. galloprovincialis. Also byssus attachment of the two species differs, P. perna being much stronger than M. galloprovincialis, which suffers high mortality due to wave action on the low shore, especially in monospecific beds. As a result, mortality of M. galloprovincialis through wave action is reduced by the presence of P. perna, which seems to confer protection against dislodgement. However, in the absence of strong wave action, P. perna competitively excludes M. galloprovincialis. Human exploitation along 160 km of coast was examined by sampling mussel populations and using aerial surveys to determine where harvesters were distributed. Collectors did not seem to discriminate between species. The study has shown that higher abundances of mussels were found in protected or inaccessible sites, while in unprotected sites mussels were scarce. Coastal nature reserves are being proven to be effective in protecting mussel populations.
- Full Text:
- Date Issued: 2005
The feeding ecology and habitat use of lions reintroduced to small, enclosed reserves in the Eastern Cape Province, South Africa
- Authors: Rapson, Jackie Anne
- Date: 2005
- Subjects: Lion -- Food -- South Africa -- Eastern Cape , Lion -- Behavior -- South Africa -- Eastern Cape , Predation (Biology) , Lion -- Nutrition
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5938 , http://hdl.handle.net/10962/d1018268
- Description: Lions have recently been introduced to a number of small (<300km²), enclosed reserves in the Eastern Cape Province as a draw-card for tourists. However, there have been few studies of lion behaviour or predator prey interactions within small reserves. Thus the aim of this study is to contribute towards a better understanding of the effects of space on diet and habitat use of re-introduced lions. The research was conducted at two small private reserves, Shamwari and Kwandwe. Kills were mostly large (55%, >120kg), adult (41%) and male (39%) and the five most commonly killed species (kudu, black wildebeest, warthog, bushbuck and Blesbuck) comprised 67.9% of all kills and 67.8% of total biomass consumed. In an attempt to overcome the problem of gaps in the kill lists, a subset of data, which excluded kills more than five days apart, was analyzed and this gave similar results to analysis of the full kill lists. Mean daily intake rates varied annually between 2.26 and 5.23kg.FEQ⁻¹ for the full data set and 4.53 and 1 0.46kg.FEQ⁻¹ for the subset. The difference between estimated (calculated using daily intake rates of 5 and 7.12kg.FEQ⁻¹) and observed offtake for each ungulate species reflected the preference ratings for that species. Analysis of bone marrow indicated that: lions killed ungulates in moderate to good condition; condition of culled animals was lower than that of kills; distal bones could be used in analyses; and that that bones exposed to the environment for at least a week could be used to analyze condition of prey. Home ranges and activity centers ranged from 21.42km² to 73.27km² and 2.7lkm² to 17.47km² respectively, which was smaller than expected from previous studies. Although, lions at K wandwe and Shan1wari preferred different vegetation types, at both reserves they generally occupied areas that were structurally similar to what has previously been reported. Continuous monitoring and analyses of diet, including condition of the prey, and habitat use will provide reserve managers with some of the necessary information to enable them to effectively manage their lion populations and to predict and minimize potential problems.
- Full Text:
- Date Issued: 2005
- Authors: Rapson, Jackie Anne
- Date: 2005
- Subjects: Lion -- Food -- South Africa -- Eastern Cape , Lion -- Behavior -- South Africa -- Eastern Cape , Predation (Biology) , Lion -- Nutrition
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5938 , http://hdl.handle.net/10962/d1018268
- Description: Lions have recently been introduced to a number of small (<300km²), enclosed reserves in the Eastern Cape Province as a draw-card for tourists. However, there have been few studies of lion behaviour or predator prey interactions within small reserves. Thus the aim of this study is to contribute towards a better understanding of the effects of space on diet and habitat use of re-introduced lions. The research was conducted at two small private reserves, Shamwari and Kwandwe. Kills were mostly large (55%, >120kg), adult (41%) and male (39%) and the five most commonly killed species (kudu, black wildebeest, warthog, bushbuck and Blesbuck) comprised 67.9% of all kills and 67.8% of total biomass consumed. In an attempt to overcome the problem of gaps in the kill lists, a subset of data, which excluded kills more than five days apart, was analyzed and this gave similar results to analysis of the full kill lists. Mean daily intake rates varied annually between 2.26 and 5.23kg.FEQ⁻¹ for the full data set and 4.53 and 1 0.46kg.FEQ⁻¹ for the subset. The difference between estimated (calculated using daily intake rates of 5 and 7.12kg.FEQ⁻¹) and observed offtake for each ungulate species reflected the preference ratings for that species. Analysis of bone marrow indicated that: lions killed ungulates in moderate to good condition; condition of culled animals was lower than that of kills; distal bones could be used in analyses; and that that bones exposed to the environment for at least a week could be used to analyze condition of prey. Home ranges and activity centers ranged from 21.42km² to 73.27km² and 2.7lkm² to 17.47km² respectively, which was smaller than expected from previous studies. Although, lions at K wandwe and Shan1wari preferred different vegetation types, at both reserves they generally occupied areas that were structurally similar to what has previously been reported. Continuous monitoring and analyses of diet, including condition of the prey, and habitat use will provide reserve managers with some of the necessary information to enable them to effectively manage their lion populations and to predict and minimize potential problems.
- Full Text:
- Date Issued: 2005
The feeding ecology, habitat selection and hunting behaviour of re-introduced cheetah on Kwandwe Private Game Reserve, Eastern Cape Province
- Authors: Bissett, Charlene
- Date: 2005
- Subjects: Kwandwe Game Reserve (South Africa) , Cheetah -- Behavior -- South Africa -- Eastern Cape , Cheetah -- Nutrition -- South Africa -- Eastern Cape , Cheetah -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5698 , http://hdl.handle.net/10962/d1005384 , Kwandwe Game Reserve (South Africa) , Cheetah -- Behavior -- South Africa -- Eastern Cape , Cheetah -- Nutrition -- South Africa -- Eastern Cape , Cheetah -- South Africa -- Eastern Cape
- Description: The re-introduction of cheetah onto small reserves in the Eastern Cape Province has created the opportunity to study this species in an environment (Valley Bushveld) in which it has not previously been studied and which is quite different from the less wooded habitats in which previous studies have occurred. Thus the aim of this study is to add to the growing number of studies of cheetah in more wooded habitats and to contribute a better understanding of the level of specialization or adaptability in space use, habitat selection, diet and hunting behaviour that the cheetah possesses. The research was conducted on Kwandwe Private Game Reserve from February 2003 to August 2004. Direct observations, scat analysis and continuous observations were used to avoid bias towards large sized prey found when only using direct observations to study diet. The cheetah killed 15 species and four of the five most important prey species (kudu, springbok, grey duiker and bushbuck) were hunted according to their abundance on the reserve, while impala, the fifth most important prey species, was avoided (i.e. preference index less than one) by the cheetah. The cheetah’s main prey was medium sized ungulates, although the three male coalition killed larger prey than females and females with cubs. The daily food intake per cheetah per day was calculated to range from 5.3kg/cheetah/day for the coalition to 8kg/cheetah/day for a solitary female. Home range areas ranged from 11.1 km² for female cheetah with cubs in a den to 65.6 km² for single female cheetah and core areas ranged from 6 km² for the three male coalition to 26.5 km² for independent cubs. Habitat selection by cheetah on Kwandwe varied between the social groups depending on their susceptibility to predation by lions, their need for cover and need for water, and was similar to what has been previously reported. The home range of the coalition incorporated the most open vegetation type (karroid shrubland) with surrounding denser vegetation, while females occupied areas of denser vegetation. Activity patterns and hunting behaviour varied between different cheetah social groups with female cheetah being more active during day light hours compared to males which made 38% of their kills after dark. The average chase distances for the various cheetah groups varied considerably, and the chase distance for successful hunts was longer than for unsuccessful for all groups except single female cheetah. The percentage of kills’ kleptoparasitised on Kwandwe was very low compared to other studies possibly due to the low density of direct competitors, which in turn lead to longer mean kill retention times. These results suggest that cheetah are more adaptable than previously thought and this adaptability may have important implications for their conservation.
- Full Text:
- Date Issued: 2005
- Authors: Bissett, Charlene
- Date: 2005
- Subjects: Kwandwe Game Reserve (South Africa) , Cheetah -- Behavior -- South Africa -- Eastern Cape , Cheetah -- Nutrition -- South Africa -- Eastern Cape , Cheetah -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5698 , http://hdl.handle.net/10962/d1005384 , Kwandwe Game Reserve (South Africa) , Cheetah -- Behavior -- South Africa -- Eastern Cape , Cheetah -- Nutrition -- South Africa -- Eastern Cape , Cheetah -- South Africa -- Eastern Cape
- Description: The re-introduction of cheetah onto small reserves in the Eastern Cape Province has created the opportunity to study this species in an environment (Valley Bushveld) in which it has not previously been studied and which is quite different from the less wooded habitats in which previous studies have occurred. Thus the aim of this study is to add to the growing number of studies of cheetah in more wooded habitats and to contribute a better understanding of the level of specialization or adaptability in space use, habitat selection, diet and hunting behaviour that the cheetah possesses. The research was conducted on Kwandwe Private Game Reserve from February 2003 to August 2004. Direct observations, scat analysis and continuous observations were used to avoid bias towards large sized prey found when only using direct observations to study diet. The cheetah killed 15 species and four of the five most important prey species (kudu, springbok, grey duiker and bushbuck) were hunted according to their abundance on the reserve, while impala, the fifth most important prey species, was avoided (i.e. preference index less than one) by the cheetah. The cheetah’s main prey was medium sized ungulates, although the three male coalition killed larger prey than females and females with cubs. The daily food intake per cheetah per day was calculated to range from 5.3kg/cheetah/day for the coalition to 8kg/cheetah/day for a solitary female. Home range areas ranged from 11.1 km² for female cheetah with cubs in a den to 65.6 km² for single female cheetah and core areas ranged from 6 km² for the three male coalition to 26.5 km² for independent cubs. Habitat selection by cheetah on Kwandwe varied between the social groups depending on their susceptibility to predation by lions, their need for cover and need for water, and was similar to what has been previously reported. The home range of the coalition incorporated the most open vegetation type (karroid shrubland) with surrounding denser vegetation, while females occupied areas of denser vegetation. Activity patterns and hunting behaviour varied between different cheetah social groups with female cheetah being more active during day light hours compared to males which made 38% of their kills after dark. The average chase distances for the various cheetah groups varied considerably, and the chase distance for successful hunts was longer than for unsuccessful for all groups except single female cheetah. The percentage of kills’ kleptoparasitised on Kwandwe was very low compared to other studies possibly due to the low density of direct competitors, which in turn lead to longer mean kill retention times. These results suggest that cheetah are more adaptable than previously thought and this adaptability may have important implications for their conservation.
- Full Text:
- Date Issued: 2005
The refinement of protective salinity guidelines for South African freshwater resources
- Authors: Slaughter, Andrew Robert
- Date: 2005
- Subjects: Water salinization -- South Africa Salinization -- Control -- South Africa Salinity -- South Africa Freshwater ecology -- South Africa Water resources development -- South Africa Caridina Caridina -- Effect of pollution
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:6031 , http://hdl.handle.net/10962/d1006170
- Description: South Africa is an arid country and its growing population is putting freshwater resources under increasing pressure. Natural salinization of freshwater systems is being exacerbated by anthropogenic influences. The National Water Act (No. 36 of 1998) stipulates the need for an ecological Reserve, that quantity and quality of freshwater needed to protect freshwater ecosystems while allowing sustainable use of freshwater resources. Water guidelines do exist in the form of the South African Water Quality Guidelines (DWAF, 1996) and more recently, Jooste and Rossouw (2002) compiled benchmark values for water quality variables marking the boundaries between ecological health classes in the 4-category classification system. Predominantly international toxicity data were used to compile the guidelines and the benchmark values. In addition, there is a paucity of chronic toxicity data nationally and internationally. This thesis showed that it is statistically possible to derive protective chronic endpoints for salinity from acute toxicity data through extrapolation. The Acute to Chronic Ratio (ACR), Two-Step Linear Regression (LRA) and Multi-Factor Probit Analysis (MPA) extrapolation methods were investigated to derive chronic toxicity data from acute toxicity data. The authors of LRA and MPA recommend associating a time independent LCx value in the range of LC₀¸₀₁ to LC₁₀ with a Predicted No Effect Concentration (PNOEC). In addition to published methods, this thesis studied the possibility of equating a time independent LC₅₀ value and subjected to a safety factor of 5 (LRA LC₅₀/5), to the PNOEC. Extrapolated chronic toxicity data where the toxicants are NaCl and Na₂SO₄ were derived for indigenous South African macroinvertebrates. NaCl and Na₂SO₄ are salts associated with salinisation in South Africa. In addition, a chronic salinity toxicity test protocol for an indigenous South African aquatic macroinvertebrate was designed and chronic toxicity test were performed using NaCl and Na₂SO₄ as toxicants. The experimental chronic toxicity data produced were used to validate results from the acute to chronic extrapolation methods. Extrapolated chronic toxicity data were inputted into Species Sensitivity Distribution curves, and concentrations that were predicted to protect 95 % of species (PC95) were compared to the sub-lethality benchmarks proposed by Jooste and Rossouw (2002) for NaCl and Na₂SO₄. This study concluded that the LRA LC₅₀/5 extrapolation method is the most protective and accurate and proposed that LRA replace the ACR method in future guideline development for inorganic salts.
- Full Text:
- Date Issued: 2005
- Authors: Slaughter, Andrew Robert
- Date: 2005
- Subjects: Water salinization -- South Africa Salinization -- Control -- South Africa Salinity -- South Africa Freshwater ecology -- South Africa Water resources development -- South Africa Caridina Caridina -- Effect of pollution
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:6031 , http://hdl.handle.net/10962/d1006170
- Description: South Africa is an arid country and its growing population is putting freshwater resources under increasing pressure. Natural salinization of freshwater systems is being exacerbated by anthropogenic influences. The National Water Act (No. 36 of 1998) stipulates the need for an ecological Reserve, that quantity and quality of freshwater needed to protect freshwater ecosystems while allowing sustainable use of freshwater resources. Water guidelines do exist in the form of the South African Water Quality Guidelines (DWAF, 1996) and more recently, Jooste and Rossouw (2002) compiled benchmark values for water quality variables marking the boundaries between ecological health classes in the 4-category classification system. Predominantly international toxicity data were used to compile the guidelines and the benchmark values. In addition, there is a paucity of chronic toxicity data nationally and internationally. This thesis showed that it is statistically possible to derive protective chronic endpoints for salinity from acute toxicity data through extrapolation. The Acute to Chronic Ratio (ACR), Two-Step Linear Regression (LRA) and Multi-Factor Probit Analysis (MPA) extrapolation methods were investigated to derive chronic toxicity data from acute toxicity data. The authors of LRA and MPA recommend associating a time independent LCx value in the range of LC₀¸₀₁ to LC₁₀ with a Predicted No Effect Concentration (PNOEC). In addition to published methods, this thesis studied the possibility of equating a time independent LC₅₀ value and subjected to a safety factor of 5 (LRA LC₅₀/5), to the PNOEC. Extrapolated chronic toxicity data where the toxicants are NaCl and Na₂SO₄ were derived for indigenous South African macroinvertebrates. NaCl and Na₂SO₄ are salts associated with salinisation in South Africa. In addition, a chronic salinity toxicity test protocol for an indigenous South African aquatic macroinvertebrate was designed and chronic toxicity test were performed using NaCl and Na₂SO₄ as toxicants. The experimental chronic toxicity data produced were used to validate results from the acute to chronic extrapolation methods. Extrapolated chronic toxicity data were inputted into Species Sensitivity Distribution curves, and concentrations that were predicted to protect 95 % of species (PC95) were compared to the sub-lethality benchmarks proposed by Jooste and Rossouw (2002) for NaCl and Na₂SO₄. This study concluded that the LRA LC₅₀/5 extrapolation method is the most protective and accurate and proposed that LRA replace the ACR method in future guideline development for inorganic salts.
- Full Text:
- Date Issued: 2005
The status and natural history of pygmy (Kogia breviceps) and dwarf (K. sima) sperm whales off Southern Africa
- Authors: Plön, Stephanie
- Date: 2005
- Subjects: Pygmy sperm whale Dwarf sperm whale Population genetics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5772 , http://hdl.handle.net/10962/d1005460
- Description: For the present study 106 strandings of Kogia breviceps and 85 strandings of K. sima along the South African coastline between 1880 and 1995 were analysed in order to examine the age and growth, male and female reproduction, diet, stranding patterns, and population genetic structure of both species. Length and weight at birth were about 120cm and 53kg for K. breviceps and about 103cm and 14kg for K. sima. Von Bertalanffy growth curves were fitted to the data and indicated that physical maturity was reached at around 15 years in both sexes of K. breviceps and at 13 years in female and 15 years in male K. sima. Asymptotic length was reached at 306.0 and 286.1cm in female and male K. breviceps and 249.14 and 263.75cm in female and male K. sima, respectively. Maximum ages were16 years for male K. breviceps and 23 years for females and 17 years for male K. sima and 22 years for females. Reversed sexual size dimorphism was suggested for K. breviceps, while in K. sima males were larger than females. Attainment of sexual maturity in males occurred at between 2.5 and 5 years of age in K. breviceps and 2.6 and 3 years in K. sima, corresponding to 241-242cm and 197cm body length, respectively. The maximum combined testis weight comprised 1.04% and 2.00% for K. breviceps and K. sima, respectively, and a polygynous mating system with a roving male strategy was proposed for both species. The sperm morphology for both Kogia species was described and is characterised by 20-25 spherical mitochondria arranged in rows around the midpiece. Attainment of sexual maturity in females occurred at 5 years in both Kogia species, and at 262cm and 215cm body length in K. breviceps and K. sima, respectively. The ovulation rates were 0.9 and 0.7 per year for K. breviceps and K. sima, respectively. In K. breviceps conceptions occurred from April to September and births from March to August, while in K. sima both conceptions and births occurred from December to March. Annual reproduction and a post-partum oestrus was suggested for both Kogia species. The diet of K. breviceps comprised 50 different cephalopod species from 22 families and 17 other prey species, while K. sima fed on 32 cephalopod species from 17 families and six others. Although niche overlap indices between the two species and between groups within each species were high, some differences in diet could be determined, which allow these two sympatrically occurring species to share the same ecological niche off the coast of Southern Africa. An analysis of the stranding patterns revealed that K. sima has a closer affinity to the Agulhas current and to higher water temperatures than K. breviceps, which is supported by differences in the size of the appendages between the two species. The population genetic analysis revealed a high haplotype and nucleotide diversity for K. breviceps in the Southern hemisphere, but a lack of significant phylogeographic structure, indicating substantial gene flow among populations and inhibiting genetic differentiation of local populations, although the South African population was somewhat isolated from others in the Southern Hemisphere. In contrast the data on the phylogeographic structure of K. sima were somewhat restrictive as the majority of the samples originated from South Africa. Nevertheless, both nucleotide and haplotype diversities were markedly lower than in K. breviceps and more similar to those for other small cetacean populations, suggesting a smaller population size for K. sima than for K. breviceps. Although both Kogia species belong to the medium to larger-sized odontocetes their life histories are located near the fast end of the slow-fast continuum of life histories of marine mammals, indicating high mortality rates. The “false-gill” marking and the ability to squirt ink are thought to reflect adaptations to predator mimicry and avoidance.
- Full Text:
- Date Issued: 2005
- Authors: Plön, Stephanie
- Date: 2005
- Subjects: Pygmy sperm whale Dwarf sperm whale Population genetics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5772 , http://hdl.handle.net/10962/d1005460
- Description: For the present study 106 strandings of Kogia breviceps and 85 strandings of K. sima along the South African coastline between 1880 and 1995 were analysed in order to examine the age and growth, male and female reproduction, diet, stranding patterns, and population genetic structure of both species. Length and weight at birth were about 120cm and 53kg for K. breviceps and about 103cm and 14kg for K. sima. Von Bertalanffy growth curves were fitted to the data and indicated that physical maturity was reached at around 15 years in both sexes of K. breviceps and at 13 years in female and 15 years in male K. sima. Asymptotic length was reached at 306.0 and 286.1cm in female and male K. breviceps and 249.14 and 263.75cm in female and male K. sima, respectively. Maximum ages were16 years for male K. breviceps and 23 years for females and 17 years for male K. sima and 22 years for females. Reversed sexual size dimorphism was suggested for K. breviceps, while in K. sima males were larger than females. Attainment of sexual maturity in males occurred at between 2.5 and 5 years of age in K. breviceps and 2.6 and 3 years in K. sima, corresponding to 241-242cm and 197cm body length, respectively. The maximum combined testis weight comprised 1.04% and 2.00% for K. breviceps and K. sima, respectively, and a polygynous mating system with a roving male strategy was proposed for both species. The sperm morphology for both Kogia species was described and is characterised by 20-25 spherical mitochondria arranged in rows around the midpiece. Attainment of sexual maturity in females occurred at 5 years in both Kogia species, and at 262cm and 215cm body length in K. breviceps and K. sima, respectively. The ovulation rates were 0.9 and 0.7 per year for K. breviceps and K. sima, respectively. In K. breviceps conceptions occurred from April to September and births from March to August, while in K. sima both conceptions and births occurred from December to March. Annual reproduction and a post-partum oestrus was suggested for both Kogia species. The diet of K. breviceps comprised 50 different cephalopod species from 22 families and 17 other prey species, while K. sima fed on 32 cephalopod species from 17 families and six others. Although niche overlap indices between the two species and between groups within each species were high, some differences in diet could be determined, which allow these two sympatrically occurring species to share the same ecological niche off the coast of Southern Africa. An analysis of the stranding patterns revealed that K. sima has a closer affinity to the Agulhas current and to higher water temperatures than K. breviceps, which is supported by differences in the size of the appendages between the two species. The population genetic analysis revealed a high haplotype and nucleotide diversity for K. breviceps in the Southern hemisphere, but a lack of significant phylogeographic structure, indicating substantial gene flow among populations and inhibiting genetic differentiation of local populations, although the South African population was somewhat isolated from others in the Southern Hemisphere. In contrast the data on the phylogeographic structure of K. sima were somewhat restrictive as the majority of the samples originated from South Africa. Nevertheless, both nucleotide and haplotype diversities were markedly lower than in K. breviceps and more similar to those for other small cetacean populations, suggesting a smaller population size for K. sima than for K. breviceps. Although both Kogia species belong to the medium to larger-sized odontocetes their life histories are located near the fast end of the slow-fast continuum of life histories of marine mammals, indicating high mortality rates. The “false-gill” marking and the ability to squirt ink are thought to reflect adaptations to predator mimicry and avoidance.
- Full Text:
- Date Issued: 2005
A comparative study of egg development in two species of Siphonariid limpets with contrasting developmental modes
- Authors: Pal, Purba
- Date: 2004
- Subjects: Siphonariidae -- Eggs Siphonariidae -- Spawning Limpets
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5826 , http://hdl.handle.net/10962/d1007663
- Description: The family Siphonariidae is considered primitive amongst the basommatophorans although the ancestry and evolutionary relationships of these marine pulmonates are far from settled. This thesis investigates and compares different aspects of egg development and the female reproductive system in two sympatric species of Siphonaria with different developmental mode (S. capensis, a planktonic developer and S. serrata, a direct developer). The study on the seasonality of gametogenesis and spawning shows that they are both spring/summer spawners with continuous sperm production. The egg production is highest in spring and summer with a brief interruption in winter months. The gametogenic cycle when examinated, reveals that both species are simultaneous hermaphrodites once sexually mature. Amongst various factors that are investigated, density of animals has a positive effect on the number of spawn only during peak spawning. Larger individuals of S. capensis and S. serrata contains more mature oocytes in the gonad indicating that the shell length and fecundity of these two limpets are positively correlated. By contrast, parasitism by trematodes has a drastic effect on the reproductive output of these limpets leaving them completely castrated. Egg development in S. capensis and S. serrata shows that both produce yolk auto synthetically (with the help of organelles like RER, Golgi bodies) but S. serrata also incorporates some high molecular weight precursors via endocytotis. The structure as well as the biochemical composition of the egg ribbons is also different between these two species with higher carbohydrate and protein content of collar shaped spawn of S. serrata. S. capensis produces egg ribbons of less fibrous nature containing thinner egg capsules compared to the direct developer. A comparison of both the glandular complex and spermatheca between these two limpets shows no inter specific difference in the structure although the glandular complex of the siphonariids shows fine structural and histochemical similarities with the albumen gland and membrane gland of the opisthobranchs. The structure of the spermatheca suggests that in both species the organ most possibly receives sperm (for degradation only?) and may transport them via the spermathecal duct (for fertilization?). Finally, it is suggested that S. capensis and S. serrata exhibit primitive features (e.g., an autosynthetic mode of vitellogenesis in S. capensis and a single glandular complex composed of an albumen and a mucous gland) compared to other basommatophorans, which should be considered in future phylogenetic investigations.
- Full Text:
- Date Issued: 2004
- Authors: Pal, Purba
- Date: 2004
- Subjects: Siphonariidae -- Eggs Siphonariidae -- Spawning Limpets
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5826 , http://hdl.handle.net/10962/d1007663
- Description: The family Siphonariidae is considered primitive amongst the basommatophorans although the ancestry and evolutionary relationships of these marine pulmonates are far from settled. This thesis investigates and compares different aspects of egg development and the female reproductive system in two sympatric species of Siphonaria with different developmental mode (S. capensis, a planktonic developer and S. serrata, a direct developer). The study on the seasonality of gametogenesis and spawning shows that they are both spring/summer spawners with continuous sperm production. The egg production is highest in spring and summer with a brief interruption in winter months. The gametogenic cycle when examinated, reveals that both species are simultaneous hermaphrodites once sexually mature. Amongst various factors that are investigated, density of animals has a positive effect on the number of spawn only during peak spawning. Larger individuals of S. capensis and S. serrata contains more mature oocytes in the gonad indicating that the shell length and fecundity of these two limpets are positively correlated. By contrast, parasitism by trematodes has a drastic effect on the reproductive output of these limpets leaving them completely castrated. Egg development in S. capensis and S. serrata shows that both produce yolk auto synthetically (with the help of organelles like RER, Golgi bodies) but S. serrata also incorporates some high molecular weight precursors via endocytotis. The structure as well as the biochemical composition of the egg ribbons is also different between these two species with higher carbohydrate and protein content of collar shaped spawn of S. serrata. S. capensis produces egg ribbons of less fibrous nature containing thinner egg capsules compared to the direct developer. A comparison of both the glandular complex and spermatheca between these two limpets shows no inter specific difference in the structure although the glandular complex of the siphonariids shows fine structural and histochemical similarities with the albumen gland and membrane gland of the opisthobranchs. The structure of the spermatheca suggests that in both species the organ most possibly receives sperm (for degradation only?) and may transport them via the spermathecal duct (for fertilization?). Finally, it is suggested that S. capensis and S. serrata exhibit primitive features (e.g., an autosynthetic mode of vitellogenesis in S. capensis and a single glandular complex composed of an albumen and a mucous gland) compared to other basommatophorans, which should be considered in future phylogenetic investigations.
- Full Text:
- Date Issued: 2004
A contribution to cabbage pest management by subsistence and small-scale farmers in the Eastern Cape, South Africa
- Authors: Mkize, Nolwazi
- Date: 2004
- Subjects: Cabbage -- Diseases and pests , Insect pests -- Integrated control -- South Africa -- Eastern Cape , Farms, Small -- South Africa -- Eastern Cape , Agriculture -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5659 , http://hdl.handle.net/10962/d1005342 , Cabbage -- Diseases and pests , Insect pests -- Integrated control -- South Africa -- Eastern Cape , Farms, Small -- South Africa -- Eastern Cape , Agriculture -- South Africa -- Eastern Cape
- Description: The interaction between farmers, agricultural scientists and extension workers is sometimes overlooked in agricultural entomology. In an attempt to respond to this reality this study examines some foundation of this interaction in relation to the pest management practices of subsistence and small-scale farmers and also highlights the problems that might arise in the implementation of IPM. Problems involving pests occurrence; language barriers; beliefs, knowledge and perception about insects, and visual literacy are examined. The thesis has a two-fold focus, firstly the study of pests on cabbages of subsistence farmers in Grahamstown and secondly a broader focus on other aspects such as cultural entomology, perception of insects and visual literacy specifically in relation to Xhosa speaking people in the Eastern Cape. The most important crop for emergent farmers in the Eastern Cape are cabbages, which have a variety of pests of which diamondback moths and are the most important. Traditional pest management practices tend to influence the development of IPM programmes adopted by these farmers. Eastern Cape farmers apply periodic cropping systems, which had an effect on the population densities of diamondback moth (DBM), other lepidopteran pests and their parasitoids. Considering the maximum population densities of DBM, which were 0.2 - 2.9 larvae/plant, there were no major pest problems. The availability of parasitoids, even in highly disturbed and patchy environments, showed good potential for biological control. Since some extension officers cannot speak the local farmers’ language, a dictionary of insect names was formulated in their language (isiXhosa) to assist communication. Response-frequency distribution analysis showed that the dictionary is essentially complete. The literal translations of some names show that isiXhosa speakers often relate insects to people, or to their habitat or classify them according to their behaviour. Farmers from eight sites in the Eastern Cape were interviewed regarding their knowledge and perception of insect pests and their control thereof. To some extent, farmers still rely on cultural control and have beliefs about insects that reflected both reality and superstition. There is no difference between the Ciskei and Transkei regions regarding insect-related beliefs. Farmers generally lack an understanding of insect ecology. There is a need for farmers to be taught about insects to assist with the implementation of IPM. Leftover pesticides from commercial farms or detergents are sometimes used to manage the pests. When training illiterate or semi-literate farmers, it is important to understand their media literacy so as to design useful graphic and object training media. Generally farmers showed that they either understand graphic or object media depending on the features of the insects being looked at. These findings are discussed with regard to the potential development of IPM training material for subsistence and small-scale farmers in a community.
- Full Text:
- Date Issued: 2004
- Authors: Mkize, Nolwazi
- Date: 2004
- Subjects: Cabbage -- Diseases and pests , Insect pests -- Integrated control -- South Africa -- Eastern Cape , Farms, Small -- South Africa -- Eastern Cape , Agriculture -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5659 , http://hdl.handle.net/10962/d1005342 , Cabbage -- Diseases and pests , Insect pests -- Integrated control -- South Africa -- Eastern Cape , Farms, Small -- South Africa -- Eastern Cape , Agriculture -- South Africa -- Eastern Cape
- Description: The interaction between farmers, agricultural scientists and extension workers is sometimes overlooked in agricultural entomology. In an attempt to respond to this reality this study examines some foundation of this interaction in relation to the pest management practices of subsistence and small-scale farmers and also highlights the problems that might arise in the implementation of IPM. Problems involving pests occurrence; language barriers; beliefs, knowledge and perception about insects, and visual literacy are examined. The thesis has a two-fold focus, firstly the study of pests on cabbages of subsistence farmers in Grahamstown and secondly a broader focus on other aspects such as cultural entomology, perception of insects and visual literacy specifically in relation to Xhosa speaking people in the Eastern Cape. The most important crop for emergent farmers in the Eastern Cape are cabbages, which have a variety of pests of which diamondback moths and are the most important. Traditional pest management practices tend to influence the development of IPM programmes adopted by these farmers. Eastern Cape farmers apply periodic cropping systems, which had an effect on the population densities of diamondback moth (DBM), other lepidopteran pests and their parasitoids. Considering the maximum population densities of DBM, which were 0.2 - 2.9 larvae/plant, there were no major pest problems. The availability of parasitoids, even in highly disturbed and patchy environments, showed good potential for biological control. Since some extension officers cannot speak the local farmers’ language, a dictionary of insect names was formulated in their language (isiXhosa) to assist communication. Response-frequency distribution analysis showed that the dictionary is essentially complete. The literal translations of some names show that isiXhosa speakers often relate insects to people, or to their habitat or classify them according to their behaviour. Farmers from eight sites in the Eastern Cape were interviewed regarding their knowledge and perception of insect pests and their control thereof. To some extent, farmers still rely on cultural control and have beliefs about insects that reflected both reality and superstition. There is no difference between the Ciskei and Transkei regions regarding insect-related beliefs. Farmers generally lack an understanding of insect ecology. There is a need for farmers to be taught about insects to assist with the implementation of IPM. Leftover pesticides from commercial farms or detergents are sometimes used to manage the pests. When training illiterate or semi-literate farmers, it is important to understand their media literacy so as to design useful graphic and object training media. Generally farmers showed that they either understand graphic or object media depending on the features of the insects being looked at. These findings are discussed with regard to the potential development of IPM training material for subsistence and small-scale farmers in a community.
- Full Text:
- Date Issued: 2004
A phenological and bioclimatic analysis of honey yield in South Africa
- Authors: Illgner, Peter Mark
- Date: 2004
- Subjects: Bee culture -- South Africa -- Statistics Honeybee -- South Africa Bee culture -- South Africa -- Management Honey plants -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5813 , http://hdl.handle.net/10962/d1007153
- Description: This study has investigated the interaction between honeybees and their forage plants and the impact of selected climatic variables on honey production in South Africa. Twenty-seven scale-hive records from 25 localities have been used as a measure of colony honey reserves. At least 944 plant species are visited by honeybees in South Africa for their nectar and/or pollen, with more than half providing both rewards. The entire honeybee flora encompasses 532 genera and 137 families. The flowering phenologies of the different reward categories of the indigenous forage plants are all significantly and positively correlated at the 0.05 level. Similarly, species offering both rewards are significantly and positively correlated with the flowering phenology of the null flora. The same results were obtained for correlations between the different reward categories of the exotic forage plants in South Africa. Of the 30 species pairs which fulfilled the criteria for selection, 23 occurred in sympatry, 5 in allopatry and 2 in possible parapatry. There is evidence for both competition and facilitation within different indigenous species pairs. The lack of geographical correlation in the intra-annual variation in honey stores and the near absence of any statistically significant (p < 0.05) honey related intra-annual intracolonial correlations may indicate that the former is more important than the latter for the determination of the level of honey reserves within a colony. Only one statistically significant correlation was found between either scale-hive record from the University of Pretoria Experimental Farm and any of the selected climatic variables. A one month lag period and/or possible seasonal effects were detected for each variable, with the exception of the duration of sunshine, in the autocorrelation analyses. A possible 12 month seasonal period was also identified in the single series fourier analyses for a number of variables. Similarly, 12 months was also the most frequently recurring period in the crossspectral results for the one scale-hive record (H42). Any activities which have an impact on the landscape have the potential to affect honeybees and/or their forage plants. Honeybee crop or plant pollination may also enhance yields for commercial farmers and facilitate rural food security.
- Full Text:
- Date Issued: 2004
- Authors: Illgner, Peter Mark
- Date: 2004
- Subjects: Bee culture -- South Africa -- Statistics Honeybee -- South Africa Bee culture -- South Africa -- Management Honey plants -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5813 , http://hdl.handle.net/10962/d1007153
- Description: This study has investigated the interaction between honeybees and their forage plants and the impact of selected climatic variables on honey production in South Africa. Twenty-seven scale-hive records from 25 localities have been used as a measure of colony honey reserves. At least 944 plant species are visited by honeybees in South Africa for their nectar and/or pollen, with more than half providing both rewards. The entire honeybee flora encompasses 532 genera and 137 families. The flowering phenologies of the different reward categories of the indigenous forage plants are all significantly and positively correlated at the 0.05 level. Similarly, species offering both rewards are significantly and positively correlated with the flowering phenology of the null flora. The same results were obtained for correlations between the different reward categories of the exotic forage plants in South Africa. Of the 30 species pairs which fulfilled the criteria for selection, 23 occurred in sympatry, 5 in allopatry and 2 in possible parapatry. There is evidence for both competition and facilitation within different indigenous species pairs. The lack of geographical correlation in the intra-annual variation in honey stores and the near absence of any statistically significant (p < 0.05) honey related intra-annual intracolonial correlations may indicate that the former is more important than the latter for the determination of the level of honey reserves within a colony. Only one statistically significant correlation was found between either scale-hive record from the University of Pretoria Experimental Farm and any of the selected climatic variables. A one month lag period and/or possible seasonal effects were detected for each variable, with the exception of the duration of sunshine, in the autocorrelation analyses. A possible 12 month seasonal period was also identified in the single series fourier analyses for a number of variables. Similarly, 12 months was also the most frequently recurring period in the crossspectral results for the one scale-hive record (H42). Any activities which have an impact on the landscape have the potential to affect honeybees and/or their forage plants. Honeybee crop or plant pollination may also enhance yields for commercial farmers and facilitate rural food security.
- Full Text:
- Date Issued: 2004
An assessment of density estimation methods for forest ungulates
- Authors: Ellis, Amanda Morgan
- Date: 2004
- Subjects: Wildlife management -- South Africa -- Eastern Cape Animal populations -- Estimates Wildlife conservation -- South Africa -- Eastern Cape Game farms -- South Africa -- Eastern Cape Ungulates -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5829 , http://hdl.handle.net/10962/d1007830
- Description: The development of conservation and management programs for an animal population relies on a knowledge of the number of individuals in an area. In order to achieve reliable estimates, precise and accurate techniques for estimating population densities are needed. This study compared the use of direct and indirect methods of estimating kudu (Trage/aphus strepsiceras), bush buck (Trage/aphus scriptus), common duiker (Sy/vicapra grimmia), and blue duiker (Philantamba manticala) densities on Shamwari Game Reserve in the Eastern Cape Province, South Africa. These species prefer habitats of dense forest and bush for concealment and are therefore not easily counted in open areas. Herein direct observation counts were compared to indirect sampling via pellet group counts (clearance plots, line transects, variable-width transects, and strip transects). Clearance plots were examined every 2 weeks, while all other methods were conducted seasonally, from August 2002 until August 2003. The strip transect method provided the lowest density estimates (animals per hal ranging from 0.001 for bushbuck to 0.025 for common duiker, while direct observations yielded the highest estimates, ranging from 0.804 for bush buck to 4.692 for kudu. Also, a validation of methods was performed against a known population of kudu during which the DISTANCE method yielded the most accurate results, with an estimated density of 0.261 that was within the actual density of 0.246 to 0.282. In addition, the DISTANCE method was compared to helicopter counts ofkudu and its estimates were found to be approximately 2.6 times greater than the helicopter count results. When the assessment of the methods was made, the cost, manpower and effort requirements, coefficient of variation, and performance against a known population for each method were taken into consideration. Overall, the DISTANCE method performed the best with low cost, minimal manpower and effort requirements, and low coefficient of variation. On Shamwari Game Reserve, the DISTANCE method estimated 0.300 kudu, 0.108 bushbuck, 0.387 common duiker, and 0.028 blue duiker per ha, which, when extrapolated to the total number of animals present within subtropical thicket habitat, estimated 1973 kudu, 710 bush buck, 2545 common duiker, and 184 blue duiker.
- Full Text:
- Date Issued: 2004
- Authors: Ellis, Amanda Morgan
- Date: 2004
- Subjects: Wildlife management -- South Africa -- Eastern Cape Animal populations -- Estimates Wildlife conservation -- South Africa -- Eastern Cape Game farms -- South Africa -- Eastern Cape Ungulates -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5829 , http://hdl.handle.net/10962/d1007830
- Description: The development of conservation and management programs for an animal population relies on a knowledge of the number of individuals in an area. In order to achieve reliable estimates, precise and accurate techniques for estimating population densities are needed. This study compared the use of direct and indirect methods of estimating kudu (Trage/aphus strepsiceras), bush buck (Trage/aphus scriptus), common duiker (Sy/vicapra grimmia), and blue duiker (Philantamba manticala) densities on Shamwari Game Reserve in the Eastern Cape Province, South Africa. These species prefer habitats of dense forest and bush for concealment and are therefore not easily counted in open areas. Herein direct observation counts were compared to indirect sampling via pellet group counts (clearance plots, line transects, variable-width transects, and strip transects). Clearance plots were examined every 2 weeks, while all other methods were conducted seasonally, from August 2002 until August 2003. The strip transect method provided the lowest density estimates (animals per hal ranging from 0.001 for bushbuck to 0.025 for common duiker, while direct observations yielded the highest estimates, ranging from 0.804 for bush buck to 4.692 for kudu. Also, a validation of methods was performed against a known population of kudu during which the DISTANCE method yielded the most accurate results, with an estimated density of 0.261 that was within the actual density of 0.246 to 0.282. In addition, the DISTANCE method was compared to helicopter counts ofkudu and its estimates were found to be approximately 2.6 times greater than the helicopter count results. When the assessment of the methods was made, the cost, manpower and effort requirements, coefficient of variation, and performance against a known population for each method were taken into consideration. Overall, the DISTANCE method performed the best with low cost, minimal manpower and effort requirements, and low coefficient of variation. On Shamwari Game Reserve, the DISTANCE method estimated 0.300 kudu, 0.108 bushbuck, 0.387 common duiker, and 0.028 blue duiker per ha, which, when extrapolated to the total number of animals present within subtropical thicket habitat, estimated 1973 kudu, 710 bush buck, 2545 common duiker, and 184 blue duiker.
- Full Text:
- Date Issued: 2004
Fishes in the Mngazi and Mngazana estuaries, with particular emphasis on the community structure and primary carbon sources
- Authors: Mbande, Sekiwe
- Date: 2004
- Subjects: Fishes -- South Africa -- Mngazi Estuary , Fishes -- South Africa -- Mngazana Estuary , Fishes -- Effect of habitat modification on -- South Africa -- Mngazi Estuary , Fishes -- Effect of habitat modification on -- South Africa -- Mngazana Estuary , Estuarine fishes -- South Africa -- Eastern Cape , Estuarine ecology -- South Africa -- Eastern Cape , Fishes -- Physiology , Fishes -- Effect of water quality on , Fishes -- Effect of temperature on , Fishes -- Food , Fishes -- Effect of turbidity on
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5700 , http://hdl.handle.net/10962/d1005386 , Fishes -- South Africa -- Mngazi Estuary , Fishes -- South Africa -- Mngazana Estuary , Fishes -- Effect of habitat modification on -- South Africa -- Mngazi Estuary , Fishes -- Effect of habitat modification on -- South Africa -- Mngazana Estuary , Estuarine fishes -- South Africa -- Eastern Cape , Estuarine ecology -- South Africa -- Eastern Cape , Fishes -- Physiology , Fishes -- Effect of water quality on , Fishes -- Effect of temperature on , Fishes -- Food , Fishes -- Effect of turbidity on
- Description: The fish community structure of two contrasting estuaries, one with a well developed mangrove forest (Mngazana) and the other without mangroves (Mngazi) was compared. Both the Mngazi and Mngazana estuary fish communities were dominated by marine species, reflecting the importance of these systems as nursery areas for marine fishes. The Mngazi Estuary contained 18% more estuarine fishes in terms of catch per unit effort (CPUE) than the Mngazana Estuary. The reduced tidal influence due to the narrow mouth opening is a possible reason for the heightened CPUE of estuarine species in the Mngazi estuary. The recorded higher diversity of fish species in the Mngazana Estuary when compared with the Mngazi Estuary was attributed to the greater influence of the marine environment due to the wide permanently open mouth, as well as the presence of a variety of habitats in this system. In both estuaries tropical and temperate species were captured, confirming the transitional nature of their biogeographic location which is situated close to the boundary between the subtropical and warm temperate regions of the Southern African coastline. Contrary to previous studies, which recorded seasonal changes in the proportions of tropical and temperate species, the proportions of tropical species remained unchanged at approximately 70% during the January and June sampling occasions. Global warming as a possible reason for the increased dominance of tropical species is discussed. Although several studies in southern Africa have investigated estuarine food web structure, none have compared mangrove and non-mangrove estuaries. In this study, the primary sources of carbon utilised by the fish fauna in the Mngazi and Mngazana estuaries was investigated. The carbon isotopic values of fishes in both estuaries displayed a continuum rather than a tight clustering around particular energy sources. Most detritus feeders of the family Mugilidae (mullets) from both estuaries were relatively more enriched than other fish taxa. The isotopic values of the mullet species suggest a diet derived from relatively enriched carbon sources such as benthic microalgae, the eelgrass Zostera capensis and associated epiphytes. Based on the isotopic values, piscivorous fishes from both estuaries could not be linked to specific prey fish taxa, but clearly the mullet species were not their main food source. The invertebrate feeders that were found in both estuaries showed greater isotopic variations in the Mngazana Estuary than in the Mngazi Estuary, probably reflecting the higher diversity of habitats (carbon sources) and invertebrate prey species in the Mngazana system. Generally the isotopic signatures of fishes from the Mngazi Estuary were more enriched than those from the Mngazana Estuary, thus indicating the possible effect of δ¹³C depleted mangrove derived carbon in the latter system.
- Full Text:
- Date Issued: 2004
- Authors: Mbande, Sekiwe
- Date: 2004
- Subjects: Fishes -- South Africa -- Mngazi Estuary , Fishes -- South Africa -- Mngazana Estuary , Fishes -- Effect of habitat modification on -- South Africa -- Mngazi Estuary , Fishes -- Effect of habitat modification on -- South Africa -- Mngazana Estuary , Estuarine fishes -- South Africa -- Eastern Cape , Estuarine ecology -- South Africa -- Eastern Cape , Fishes -- Physiology , Fishes -- Effect of water quality on , Fishes -- Effect of temperature on , Fishes -- Food , Fishes -- Effect of turbidity on
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5700 , http://hdl.handle.net/10962/d1005386 , Fishes -- South Africa -- Mngazi Estuary , Fishes -- South Africa -- Mngazana Estuary , Fishes -- Effect of habitat modification on -- South Africa -- Mngazi Estuary , Fishes -- Effect of habitat modification on -- South Africa -- Mngazana Estuary , Estuarine fishes -- South Africa -- Eastern Cape , Estuarine ecology -- South Africa -- Eastern Cape , Fishes -- Physiology , Fishes -- Effect of water quality on , Fishes -- Effect of temperature on , Fishes -- Food , Fishes -- Effect of turbidity on
- Description: The fish community structure of two contrasting estuaries, one with a well developed mangrove forest (Mngazana) and the other without mangroves (Mngazi) was compared. Both the Mngazi and Mngazana estuary fish communities were dominated by marine species, reflecting the importance of these systems as nursery areas for marine fishes. The Mngazi Estuary contained 18% more estuarine fishes in terms of catch per unit effort (CPUE) than the Mngazana Estuary. The reduced tidal influence due to the narrow mouth opening is a possible reason for the heightened CPUE of estuarine species in the Mngazi estuary. The recorded higher diversity of fish species in the Mngazana Estuary when compared with the Mngazi Estuary was attributed to the greater influence of the marine environment due to the wide permanently open mouth, as well as the presence of a variety of habitats in this system. In both estuaries tropical and temperate species were captured, confirming the transitional nature of their biogeographic location which is situated close to the boundary between the subtropical and warm temperate regions of the Southern African coastline. Contrary to previous studies, which recorded seasonal changes in the proportions of tropical and temperate species, the proportions of tropical species remained unchanged at approximately 70% during the January and June sampling occasions. Global warming as a possible reason for the increased dominance of tropical species is discussed. Although several studies in southern Africa have investigated estuarine food web structure, none have compared mangrove and non-mangrove estuaries. In this study, the primary sources of carbon utilised by the fish fauna in the Mngazi and Mngazana estuaries was investigated. The carbon isotopic values of fishes in both estuaries displayed a continuum rather than a tight clustering around particular energy sources. Most detritus feeders of the family Mugilidae (mullets) from both estuaries were relatively more enriched than other fish taxa. The isotopic values of the mullet species suggest a diet derived from relatively enriched carbon sources such as benthic microalgae, the eelgrass Zostera capensis and associated epiphytes. Based on the isotopic values, piscivorous fishes from both estuaries could not be linked to specific prey fish taxa, but clearly the mullet species were not their main food source. The invertebrate feeders that were found in both estuaries showed greater isotopic variations in the Mngazana Estuary than in the Mngazi Estuary, probably reflecting the higher diversity of habitats (carbon sources) and invertebrate prey species in the Mngazana system. Generally the isotopic signatures of fishes from the Mngazi Estuary were more enriched than those from the Mngazana Estuary, thus indicating the possible effect of δ¹³C depleted mangrove derived carbon in the latter system.
- Full Text:
- Date Issued: 2004
Geographic susceptibility of Helicoverpa armigera (Lepidoptera: Noctuidae) to insecticidal proteins in Bt-cotton in South Africa
- Van Jaarsveld, Martha Johanna
- Authors: Van Jaarsveld, Martha Johanna
- Date: 2004
- Subjects: Helicoverpa armigera , Noctuidae , Lepidoptera , Cotton -- Diseases and pests -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5701 , http://hdl.handle.net/10962/d1005387 , Helicoverpa armigera , Noctuidae , Lepidoptera , Cotton -- Diseases and pests -- South Africa
- Description: Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) (African bollworm) is a typical noctuid with a very catholic taste in food plants and whose larvae feed on a wide range of cultivated and wild plants. It has been identified as the most polyphagous and injurious pest in South Africa. Helicoverpa armigera is also a key pest of cotton in many parts of the world. This key pest requires extensive control as it adversely effects yield and has built up resistance to synthetic pyrethroid insecticides. Cotton is an important crop produced by commercial and small-scale farmers in South Africa. The local demand for cotton has not been exceeded yet, but to satisfy a demanding market, pest control costs play an important role in cotton production. The threat of an insect pest that has already shown resistance prompted the present study to investigate the possibility of resistance to Bt-cotton. Genetically engineered or Bt-cotton was introduced commercially in 1996 in South Africa. All Bt-cotton plants contain one or more foreign genes derived from the soil-dwelling bacterium, Bacillus thuringiensis (Berliner), which produces protein crystals. These crystals were isolated and transferred into the genome of a cotton plant resulting in the plant producing it’s own protein insecticide. In 1998, Monsanto (Pty) Ltd requested research into the geographic susceptibility of H. armigera to the insecticidal proteins in Bt-cotton in SA. Laboratory reared and field sampled populations of H. armigera were exposed to a diet mixed with various baseline concentrations of the Bt-gene Cry1Ac freeze dried protein. This study also determined the performance of H. armigera and Spodoptera littoralis (Boisduval) on different Bt-cotton field cultivars containing different Cry-protein genes. Results obtained indicated a significant difference in susceptibility in two field populations of H. armigera to the Bt-protein Cry1Ac, even though the LD50,s in the 2003 season did not indicate resistance. Bt-cotton cultivar 15985 BX controlled H. armigera and S. littoralis larvae, the best followed in descending order by cultivar 15985 X, 15985 B and DP50 B. Results on H. armigera also indicated that the Cry-proteins in the plant parts of the different cultivars did not diminish as the season progressed. The Bt-cotton cultivars induced retarded growth of larvae, due to either a repellent effect or lack of feeding by larvae. Widespread adoption of Bt-cotton by South African farmers led to regional declines in bollworm populations, reduced insecticide use, and increased yields. Genetically modified crops therefore contribute to a cost effective, sustainable, productive and efficient form of agriculture, with a resultant positive impact on the environment. As the market for commercial Bt-cotton in South Africa expands, it is recommended that a monitoring programme for potential resistant genes in H. armigera should be implemented at least every 2 - 3 years. This will ensure that effective resistance management strategies are utilised. Coupled with this are the Biosafety Risks regarding the effect of new proteins expressed in transgenic plants, which require further studies.
- Full Text:
- Date Issued: 2004
- Authors: Van Jaarsveld, Martha Johanna
- Date: 2004
- Subjects: Helicoverpa armigera , Noctuidae , Lepidoptera , Cotton -- Diseases and pests -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5701 , http://hdl.handle.net/10962/d1005387 , Helicoverpa armigera , Noctuidae , Lepidoptera , Cotton -- Diseases and pests -- South Africa
- Description: Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) (African bollworm) is a typical noctuid with a very catholic taste in food plants and whose larvae feed on a wide range of cultivated and wild plants. It has been identified as the most polyphagous and injurious pest in South Africa. Helicoverpa armigera is also a key pest of cotton in many parts of the world. This key pest requires extensive control as it adversely effects yield and has built up resistance to synthetic pyrethroid insecticides. Cotton is an important crop produced by commercial and small-scale farmers in South Africa. The local demand for cotton has not been exceeded yet, but to satisfy a demanding market, pest control costs play an important role in cotton production. The threat of an insect pest that has already shown resistance prompted the present study to investigate the possibility of resistance to Bt-cotton. Genetically engineered or Bt-cotton was introduced commercially in 1996 in South Africa. All Bt-cotton plants contain one or more foreign genes derived from the soil-dwelling bacterium, Bacillus thuringiensis (Berliner), which produces protein crystals. These crystals were isolated and transferred into the genome of a cotton plant resulting in the plant producing it’s own protein insecticide. In 1998, Monsanto (Pty) Ltd requested research into the geographic susceptibility of H. armigera to the insecticidal proteins in Bt-cotton in SA. Laboratory reared and field sampled populations of H. armigera were exposed to a diet mixed with various baseline concentrations of the Bt-gene Cry1Ac freeze dried protein. This study also determined the performance of H. armigera and Spodoptera littoralis (Boisduval) on different Bt-cotton field cultivars containing different Cry-protein genes. Results obtained indicated a significant difference in susceptibility in two field populations of H. armigera to the Bt-protein Cry1Ac, even though the LD50,s in the 2003 season did not indicate resistance. Bt-cotton cultivar 15985 BX controlled H. armigera and S. littoralis larvae, the best followed in descending order by cultivar 15985 X, 15985 B and DP50 B. Results on H. armigera also indicated that the Cry-proteins in the plant parts of the different cultivars did not diminish as the season progressed. The Bt-cotton cultivars induced retarded growth of larvae, due to either a repellent effect or lack of feeding by larvae. Widespread adoption of Bt-cotton by South African farmers led to regional declines in bollworm populations, reduced insecticide use, and increased yields. Genetically modified crops therefore contribute to a cost effective, sustainable, productive and efficient form of agriculture, with a resultant positive impact on the environment. As the market for commercial Bt-cotton in South Africa expands, it is recommended that a monitoring programme for potential resistant genes in H. armigera should be implemented at least every 2 - 3 years. This will ensure that effective resistance management strategies are utilised. Coupled with this are the Biosafety Risks regarding the effect of new proteins expressed in transgenic plants, which require further studies.
- Full Text:
- Date Issued: 2004
Investigation of the larval parasitoids of the false codling moth, Cryptophlebia Leucotreta (Meyrick) (Lepidoptera: Tortricidae), on citrus in South Africa
- Authors: Sishuba, Nomahlubi
- Date: 2004
- Subjects: Cryptophlebia leucotreta , Tortricidae , Cryptophlebia leucotreta -- Control , Pests -- Biological control , Parasitoids , Citrus -- Diseases and pests
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5918 , http://hdl.handle.net/10962/d1016267
- Description: The study examined the larval parasitoids of Cryptophlebia leucotreta (Meyrick) on citrus in South Africa and aimed to improve the existing rearing techniques of C. leucotreta with a view to mass rearing of biological control agents. The biological characteristics of Agathis bishopi Nixon (Hymenoptera: Braconidae) were studied, with an emphasis on parasitism rates in the field, host stage preference, developmental rate, life span and offspring sex ratios. Two larval parasitoids, A. bishopi and Apophua leucotretae (Wilkinson) (Hymenoptera: Ichneumonidae), and an egg parasitoid, Trichogrammatoidea cryptophlebiae Nagaraja (Hymenoptera: Trichogrammatidae), were recorded on C. leucotreta on citrus. A. bishopi was the more abundant of the larval parasitoids and exhibited density dependent parasitism. The highest parasitism rates were observed in December with up to 38% in Sundays River Valley and 34% in Gamtoos River Valley, at a time when the highest false codling moth infestations were observed. Agathis bishopi was recorded only in the Eastern Cape Province. The sex ratio of A. bishopi was biased towards females throughout the study (77% in Gamtoos River Valley and 72% in Sundays River Valley). Agathis bishopi is a solitary, koinobiont, larval-pupal endoparasitoid. The species showed a preference for 1st and 2"d instar hosts. Females regulate the sex of their progeny according to the size and larval stage of the host, ovipositing unfertilised eggs in younger, smaller larvae (1st instars) and fertilised eggs in older, larger larvae (2nd instars). The developmental rate of A. bishopi is in synchrony with that of the moth and adults emerge when adult moths that have escaped parasitism emerge. Agathis bishopi and T. cryptophlebiae compliment each other because they have different niches and strategies of attack. Integrating A. bishopi and T. cryptophlebiae into the management of citrus orchards has potential to suppress false codling moth. Larger rearing containers seemed ideal for large-scale rearing of false codling moth. A higher percentage of adults was obtained from larvae reared in larger containers than in smaller ones. The width of the sponges used as stoppers prevented escape of the larvae. Media prepared in larger containers are easier and simpler to prepare than in smaller ones, thus eliminating many precautions otherwise necessary to prevent contamination. Moth production was greatly reduced by the high concentration of Sporekill used for egg decontamination.
- Full Text:
- Date Issued: 2004
- Authors: Sishuba, Nomahlubi
- Date: 2004
- Subjects: Cryptophlebia leucotreta , Tortricidae , Cryptophlebia leucotreta -- Control , Pests -- Biological control , Parasitoids , Citrus -- Diseases and pests
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5918 , http://hdl.handle.net/10962/d1016267
- Description: The study examined the larval parasitoids of Cryptophlebia leucotreta (Meyrick) on citrus in South Africa and aimed to improve the existing rearing techniques of C. leucotreta with a view to mass rearing of biological control agents. The biological characteristics of Agathis bishopi Nixon (Hymenoptera: Braconidae) were studied, with an emphasis on parasitism rates in the field, host stage preference, developmental rate, life span and offspring sex ratios. Two larval parasitoids, A. bishopi and Apophua leucotretae (Wilkinson) (Hymenoptera: Ichneumonidae), and an egg parasitoid, Trichogrammatoidea cryptophlebiae Nagaraja (Hymenoptera: Trichogrammatidae), were recorded on C. leucotreta on citrus. A. bishopi was the more abundant of the larval parasitoids and exhibited density dependent parasitism. The highest parasitism rates were observed in December with up to 38% in Sundays River Valley and 34% in Gamtoos River Valley, at a time when the highest false codling moth infestations were observed. Agathis bishopi was recorded only in the Eastern Cape Province. The sex ratio of A. bishopi was biased towards females throughout the study (77% in Gamtoos River Valley and 72% in Sundays River Valley). Agathis bishopi is a solitary, koinobiont, larval-pupal endoparasitoid. The species showed a preference for 1st and 2"d instar hosts. Females regulate the sex of their progeny according to the size and larval stage of the host, ovipositing unfertilised eggs in younger, smaller larvae (1st instars) and fertilised eggs in older, larger larvae (2nd instars). The developmental rate of A. bishopi is in synchrony with that of the moth and adults emerge when adult moths that have escaped parasitism emerge. Agathis bishopi and T. cryptophlebiae compliment each other because they have different niches and strategies of attack. Integrating A. bishopi and T. cryptophlebiae into the management of citrus orchards has potential to suppress false codling moth. Larger rearing containers seemed ideal for large-scale rearing of false codling moth. A higher percentage of adults was obtained from larvae reared in larger containers than in smaller ones. The width of the sponges used as stoppers prevented escape of the larvae. Media prepared in larger containers are easier and simpler to prepare than in smaller ones, thus eliminating many precautions otherwise necessary to prevent contamination. Moth production was greatly reduced by the high concentration of Sporekill used for egg decontamination.
- Full Text:
- Date Issued: 2004