A multidisciplinary study to assess the ecology of the Cape sea urchin, Parechinus angulosus, and its emerging use as a bioindicator to monitor coastal resiliency
- Authors: Redelinghuys, Suzanne
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435691 , vital:73179 , DOI 10.21504/10962/435692
- Description: The resilience of marine species in the face of unpredictable climate change stands as a paramount concern for the maintenance of ecological stability. Under such fluctuating conditions, it is critical to understand how organisms mitigate these effects in physiological, genetic, and morphological terms. To that end, this thesis focused on the Cape sea urchin, Parechinus angulosus, employing a multidisciplinary approach encompassing morphology, genomics, and gut microbial diversity to assess its potential as a bioindicator species and elucidate its adaptive strategies in response to varying environmental conditions along the South African coastline. This was achieved through studying their anatomy in order to link observed variation to prevailing local environmental conditions, aided by the species’ wide distribution range which allows insight into adaptations across broad geographic regions and ecological settings. The first empirical chapter, Chapter 3, focusing on eight key morphometric traits of test, Aristotle’s lantern and spines, revealed distinct variation in the Cape sea urchin's morphology between the east and west coasts of South Africa, suggesting the presence of some level of local adaptation to the prevailing environmental factors found on the east and west coasts of South Africa. This points to potential bioindicator capabilities of the species, reflecting adaptive divergence amidst contrasting environmental conditions. Further analysis is however necessary in order to isolate specific physiological trends that may be associated with these morphometric differences, thereby enhancing and tightening their ecological implications. The second experimental chapter, Chapter 4, delved into the genetic structure of the Cape urchin by investigating genome-wide diversity, the presence of cryptic population structure, and spatial patterns of genomic diversity. Moderate genomic differentiation was detected among populations along the eastern and western coasts of South Africa by outlier loci that may undergo natural selection, which could indicate local adaptation to environmental conditions. This pattern hints at adaptive differentiation and cryptic genetic structures within the Cape sea urchin populations and emphasises the species' potential adaptive responses to localised (in this case regional) environmental pressures. Assigning functional significance to these genetic variations will require a comprehensive annotated reference genome, a limitation acknowledged in the current study. Chapter 5 explored the gut microbial diversity and revealed significant compositional variations between the east and west coast populations of South Africa, confirming regional and inter-regional variation. This chapter also highlighted the essential biochemical pathways critical to the survival of the host which is crucial for assessing the health of the urchin host. Together, the functional content of the gut bacteria and microbial diversity showcases its potential as a bioindicator for coastal ecosystem health. Logistical challenges and confounding factors like host physiology will need to be fully considered for its effective application. Overall, the findings of this doctoral research suggest that the Cape sea urchin displays promising characteristics as a bioindicator species due to its morphological, genetic, and gut microbial variations in response to environmental differences, providing a diverse array of means in which urchins could be used as bioindicators, from their uses to assess water quality and detect pollution, to ecosystem health monitoring and biodiversity studies in which sea urchin abundance, distribution, and presence are monitored. Further research, integrating these multidisciplinary approaches is recommended to validate and refine its bioindicator potential. Additionally, the development of a comprehensive annotated reference genome is imperative to harness the species' genetic information effectively. This study underscores the significance of integrating multiple disciplines in understanding how species respond to environmental change and their potentials contributions to monitor ecological resilience. The original multidisciplinary approach, combined with high computational outputs presents a promising framework for a comprehensive ecological monitoring in marine ecosystems. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
- Authors: Redelinghuys, Suzanne
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435691 , vital:73179 , DOI 10.21504/10962/435692
- Description: The resilience of marine species in the face of unpredictable climate change stands as a paramount concern for the maintenance of ecological stability. Under such fluctuating conditions, it is critical to understand how organisms mitigate these effects in physiological, genetic, and morphological terms. To that end, this thesis focused on the Cape sea urchin, Parechinus angulosus, employing a multidisciplinary approach encompassing morphology, genomics, and gut microbial diversity to assess its potential as a bioindicator species and elucidate its adaptive strategies in response to varying environmental conditions along the South African coastline. This was achieved through studying their anatomy in order to link observed variation to prevailing local environmental conditions, aided by the species’ wide distribution range which allows insight into adaptations across broad geographic regions and ecological settings. The first empirical chapter, Chapter 3, focusing on eight key morphometric traits of test, Aristotle’s lantern and spines, revealed distinct variation in the Cape sea urchin's morphology between the east and west coasts of South Africa, suggesting the presence of some level of local adaptation to the prevailing environmental factors found on the east and west coasts of South Africa. This points to potential bioindicator capabilities of the species, reflecting adaptive divergence amidst contrasting environmental conditions. Further analysis is however necessary in order to isolate specific physiological trends that may be associated with these morphometric differences, thereby enhancing and tightening their ecological implications. The second experimental chapter, Chapter 4, delved into the genetic structure of the Cape urchin by investigating genome-wide diversity, the presence of cryptic population structure, and spatial patterns of genomic diversity. Moderate genomic differentiation was detected among populations along the eastern and western coasts of South Africa by outlier loci that may undergo natural selection, which could indicate local adaptation to environmental conditions. This pattern hints at adaptive differentiation and cryptic genetic structures within the Cape sea urchin populations and emphasises the species' potential adaptive responses to localised (in this case regional) environmental pressures. Assigning functional significance to these genetic variations will require a comprehensive annotated reference genome, a limitation acknowledged in the current study. Chapter 5 explored the gut microbial diversity and revealed significant compositional variations between the east and west coast populations of South Africa, confirming regional and inter-regional variation. This chapter also highlighted the essential biochemical pathways critical to the survival of the host which is crucial for assessing the health of the urchin host. Together, the functional content of the gut bacteria and microbial diversity showcases its potential as a bioindicator for coastal ecosystem health. Logistical challenges and confounding factors like host physiology will need to be fully considered for its effective application. Overall, the findings of this doctoral research suggest that the Cape sea urchin displays promising characteristics as a bioindicator species due to its morphological, genetic, and gut microbial variations in response to environmental differences, providing a diverse array of means in which urchins could be used as bioindicators, from their uses to assess water quality and detect pollution, to ecosystem health monitoring and biodiversity studies in which sea urchin abundance, distribution, and presence are monitored. Further research, integrating these multidisciplinary approaches is recommended to validate and refine its bioindicator potential. Additionally, the development of a comprehensive annotated reference genome is imperative to harness the species' genetic information effectively. This study underscores the significance of integrating multiple disciplines in understanding how species respond to environmental change and their potentials contributions to monitor ecological resilience. The original multidisciplinary approach, combined with high computational outputs presents a promising framework for a comprehensive ecological monitoring in marine ecosystems. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
Augmentation of Aphytis melinus DeBach (Hymenoptera: Aphelinidae) for the control of California red scale Aonidiella aurantii Maskell (Hemiptera: Diaspididae) on citrus
- De Beer, Ernst Friedrich Ludwig
- Authors: De Beer, Ernst Friedrich Ludwig
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435638 , vital:73175 , DOI 10.21504/10962/435638
- Description: Aphytis lingnanensis was reared and tested in South Africa in the early 2000s for augmentation against red scale on citrus and was found to be ineffective. Aphytis melinus is now commercially available and it is important that the efficacy of augmentation thereof on red scale is determined locally. Field trials, fitness assessments and molecular identification on A. melinus from two insectaries were done. Field trials was done in seven, five and six pairs of comparable release and control orchards across the Eastern and Western Cape during the seasons of 2019/2020, 2020/2021 and 2021/2022 respectively. Red scale infestation was monitored and a sample of 20 infested fruit from each orchard was randomly collected every four weeks. Aphytis spp. responsible for parasitism were identified and the percentage parasitism recorded. Results of this study of field trials suggest that the augmentation of A. melinus did not significantly increase the level of parasitism above that of the untreated control. Five repetitions with six replicates of flight and longevity tests were performed with wasps from each insectary. Wasps in the longevity test from two insectaries were kept at 23 °C and 65% RH with honey. Flight tests were performed in tubes of 16 by 30 cm, with a light above a clear, sticky ceiling at 23 °C and 65% RH. On average in five replicates, 65%, 33% and 17% A. melinus wasps were alive on day one, five and 10 respectively. The overall sex ratio was 1.58 for females to males, but 1.05, 2.19 and 2.66 for non-flyers, non-crawlers, crawlers, and flyers respectively. In flight tests for both insectaries combined, only 36.97% of wasps could initiate flight in 24 h while 56.96% remained on the tube floor, and 6.05% attempted to crawl upwards. No significant differences in flight performance were recorded between the two insectaries. Wasps from the local insectary lived significantly longer during the longevity tests but were shorter in transit than wasps from the overseas insectary. COI genes were sequenced and compared against Genbank sequences using BLAST. Molecular identifications did not confirm morphological identifications for all species, indicating unexpected genetic complexity. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
- Authors: De Beer, Ernst Friedrich Ludwig
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435638 , vital:73175 , DOI 10.21504/10962/435638
- Description: Aphytis lingnanensis was reared and tested in South Africa in the early 2000s for augmentation against red scale on citrus and was found to be ineffective. Aphytis melinus is now commercially available and it is important that the efficacy of augmentation thereof on red scale is determined locally. Field trials, fitness assessments and molecular identification on A. melinus from two insectaries were done. Field trials was done in seven, five and six pairs of comparable release and control orchards across the Eastern and Western Cape during the seasons of 2019/2020, 2020/2021 and 2021/2022 respectively. Red scale infestation was monitored and a sample of 20 infested fruit from each orchard was randomly collected every four weeks. Aphytis spp. responsible for parasitism were identified and the percentage parasitism recorded. Results of this study of field trials suggest that the augmentation of A. melinus did not significantly increase the level of parasitism above that of the untreated control. Five repetitions with six replicates of flight and longevity tests were performed with wasps from each insectary. Wasps in the longevity test from two insectaries were kept at 23 °C and 65% RH with honey. Flight tests were performed in tubes of 16 by 30 cm, with a light above a clear, sticky ceiling at 23 °C and 65% RH. On average in five replicates, 65%, 33% and 17% A. melinus wasps were alive on day one, five and 10 respectively. The overall sex ratio was 1.58 for females to males, but 1.05, 2.19 and 2.66 for non-flyers, non-crawlers, crawlers, and flyers respectively. In flight tests for both insectaries combined, only 36.97% of wasps could initiate flight in 24 h while 56.96% remained on the tube floor, and 6.05% attempted to crawl upwards. No significant differences in flight performance were recorded between the two insectaries. Wasps from the local insectary lived significantly longer during the longevity tests but were shorter in transit than wasps from the overseas insectary. COI genes were sequenced and compared against Genbank sequences using BLAST. Molecular identifications did not confirm morphological identifications for all species, indicating unexpected genetic complexity. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
Developing biological control agents for the management of the invasive tree Robinia pseudoacacia
- Authors: Wolmarans, Abigail
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435717 , vital:73181 , DOI 10.21504/10962/435717
- Description: Robinia pseudoacacia (Fabaceae) is a deciduous tree native to the Appalachian Mountains of North America but has become naturalised and invasive in other countries such as temperate North America, Europe, Australia, and Southern Africa. In South Africa the tree is classified as a category 1B invasive alien under the National Environmental Management Act (NEMBA), which stipulates the species requires some form of control as it has already caused extensive negative ecological and economic impacts. In the invaded range the tree creates monocultures that displace native species and spreads rapidly from suckering roots, making it a proficient invader. The South Africa plant prioritisation system suggests R. pseudoacacia is in the top three species which should be considered for classical weed biological control in South Africa. This thesis investigates which insects known to be associated with tree should be prioritised as candidate agents, as well as offering interesting insights into prioritising insects for weed biological control and using plant phylogenies and available literature to predict insect specificity. To ensure that no candidate biological control agents were already present in South Africa as well as to prioritise which guilds of the tree to prioritise for potential biological control, pre-release surveys were conducted across nine sites where the tree has invaded South Africa. It was found that no insects from the native range of R. pseudoacacia were present in South Africa. Seed surveys revealed that generalist insects attack a sizable proportion (68 %) of the seeds on the trees. In combination with a low seed soil bank (15.8 %) this suggests that seed- feeding agents may be helpful, however, candidate agents which damage leaves should be prioritised due to R. pseudoacacia relying heavily upon vegetative reproduction and much less on sexual reproduction. Leaves may therefor reduce the spread of these invasive trees. The insect assemblages in the native range of R. pseudoacacia are well understood. In addition, several associated insects have unintentionally followed the tree on its global spread, where they are often regarded as pests. The third chapter is therefore aimed at prioritising the known insects associated with the tree in both the native and invaded range. Literature surveys and Harris (1973) prioritisation systems were used to prioritise close to 64 candidate biological control agents down to three foliage- feeding agents, namely Odontota dorsalis (Coleoptera, Chrysomelidae), Macrosaccus robiniella (Lepidoptera, Gracillariidae) and Obolodiplosis robiniae (Diptera, Cecidomyiidae). To further prioritise the six selected agents in Chapter 4, species distribution were modelled with known climatic variables. This was done by using the species known occurrence localities, from both the native and where applicable invaded ranges, to identify which species that would best match with South Africa’s climate. The study showed that O. dorsalis is best suited to survive in South Africa, followed by O. robiniae and then M. robiniella. Collected data aided in the introduction of the prioritised O. robiniae into South African quarantine facilities. Unfortunately, despite several attempts, cultures could not be established, making conventional host specificity testing impossible. Thus, in Chapter 5 we aimed to determine the potential host range of the midge using information from experts in the field of galling insects, literature surveys, agricultural pest lists, and social science platforms coupled with native and invaded range surveys. The list of non-target species to consider as potential hosts was refined by developing phylogenetic trees of closely related Fabaceae that share the same distribution (native and invaded) as R. pseudoacacia. Through the available information gathered, and field surveys of these species, O. robiniae has not been shown to utilise any species, other than those from the Robinoid clade. In addition, the midge has also never been recorded on a number of closely related leguminous fodder and horticultural species growing in close proximity at high densities to R. pseudoacacia - suggesting negligible risk to South African growers of the same species. Host-specificity assessments through field surveys can be regarded as one of the best indicators of the ecological host range, however, this information is difficult to quantify and infrequently available, thus seldom used when determining the safety of a candidate biocontrol agent. In this unique study, using the extensive data collected we are able to show that O. robiniae would be potentially safe for release in South Africa. However, open field tests exposing closely related non-target plant species under natural and semi-natural conditions are planned in Switzerland over the next two years aiming to confirm these conclusions. By identifying and prioritizing potential biological control agents, this research contributes to the development of a targeted and sustainable solution for managing R. pseudoacacia in South Africa. The economic implications of successful biological control include reduced costs associated with invasive species management and ecosystem restoration. Additionally, by mitigating the negative ecological impacts, the agricultural systems in affected regions stand to benefit from enhanced resilience and productivity. Furthermore, the study's approach of using plant phylogenies and available literature to predict insect specificity offers a valuable methodological contribution to the broader field of weed biological control. This methodology can be adapted and applied to other invasive species, providing a framework for efficient and informed decision-making in weed management strategies. In essence, this research not only addresses the specific challenges posed by R. pseudoacacia in South Africa but also provides a template for tackling similar issues in different geographical contexts, thereby contributing to global efforts in sustainable agriculture and environmental conservation. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
- Authors: Wolmarans, Abigail
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435717 , vital:73181 , DOI 10.21504/10962/435717
- Description: Robinia pseudoacacia (Fabaceae) is a deciduous tree native to the Appalachian Mountains of North America but has become naturalised and invasive in other countries such as temperate North America, Europe, Australia, and Southern Africa. In South Africa the tree is classified as a category 1B invasive alien under the National Environmental Management Act (NEMBA), which stipulates the species requires some form of control as it has already caused extensive negative ecological and economic impacts. In the invaded range the tree creates monocultures that displace native species and spreads rapidly from suckering roots, making it a proficient invader. The South Africa plant prioritisation system suggests R. pseudoacacia is in the top three species which should be considered for classical weed biological control in South Africa. This thesis investigates which insects known to be associated with tree should be prioritised as candidate agents, as well as offering interesting insights into prioritising insects for weed biological control and using plant phylogenies and available literature to predict insect specificity. To ensure that no candidate biological control agents were already present in South Africa as well as to prioritise which guilds of the tree to prioritise for potential biological control, pre-release surveys were conducted across nine sites where the tree has invaded South Africa. It was found that no insects from the native range of R. pseudoacacia were present in South Africa. Seed surveys revealed that generalist insects attack a sizable proportion (68 %) of the seeds on the trees. In combination with a low seed soil bank (15.8 %) this suggests that seed- feeding agents may be helpful, however, candidate agents which damage leaves should be prioritised due to R. pseudoacacia relying heavily upon vegetative reproduction and much less on sexual reproduction. Leaves may therefor reduce the spread of these invasive trees. The insect assemblages in the native range of R. pseudoacacia are well understood. In addition, several associated insects have unintentionally followed the tree on its global spread, where they are often regarded as pests. The third chapter is therefore aimed at prioritising the known insects associated with the tree in both the native and invaded range. Literature surveys and Harris (1973) prioritisation systems were used to prioritise close to 64 candidate biological control agents down to three foliage- feeding agents, namely Odontota dorsalis (Coleoptera, Chrysomelidae), Macrosaccus robiniella (Lepidoptera, Gracillariidae) and Obolodiplosis robiniae (Diptera, Cecidomyiidae). To further prioritise the six selected agents in Chapter 4, species distribution were modelled with known climatic variables. This was done by using the species known occurrence localities, from both the native and where applicable invaded ranges, to identify which species that would best match with South Africa’s climate. The study showed that O. dorsalis is best suited to survive in South Africa, followed by O. robiniae and then M. robiniella. Collected data aided in the introduction of the prioritised O. robiniae into South African quarantine facilities. Unfortunately, despite several attempts, cultures could not be established, making conventional host specificity testing impossible. Thus, in Chapter 5 we aimed to determine the potential host range of the midge using information from experts in the field of galling insects, literature surveys, agricultural pest lists, and social science platforms coupled with native and invaded range surveys. The list of non-target species to consider as potential hosts was refined by developing phylogenetic trees of closely related Fabaceae that share the same distribution (native and invaded) as R. pseudoacacia. Through the available information gathered, and field surveys of these species, O. robiniae has not been shown to utilise any species, other than those from the Robinoid clade. In addition, the midge has also never been recorded on a number of closely related leguminous fodder and horticultural species growing in close proximity at high densities to R. pseudoacacia - suggesting negligible risk to South African growers of the same species. Host-specificity assessments through field surveys can be regarded as one of the best indicators of the ecological host range, however, this information is difficult to quantify and infrequently available, thus seldom used when determining the safety of a candidate biocontrol agent. In this unique study, using the extensive data collected we are able to show that O. robiniae would be potentially safe for release in South Africa. However, open field tests exposing closely related non-target plant species under natural and semi-natural conditions are planned in Switzerland over the next two years aiming to confirm these conclusions. By identifying and prioritizing potential biological control agents, this research contributes to the development of a targeted and sustainable solution for managing R. pseudoacacia in South Africa. The economic implications of successful biological control include reduced costs associated with invasive species management and ecosystem restoration. Additionally, by mitigating the negative ecological impacts, the agricultural systems in affected regions stand to benefit from enhanced resilience and productivity. Furthermore, the study's approach of using plant phylogenies and available literature to predict insect specificity offers a valuable methodological contribution to the broader field of weed biological control. This methodology can be adapted and applied to other invasive species, providing a framework for efficient and informed decision-making in weed management strategies. In essence, this research not only addresses the specific challenges posed by R. pseudoacacia in South Africa but also provides a template for tackling similar issues in different geographical contexts, thereby contributing to global efforts in sustainable agriculture and environmental conservation. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
Ecological impacts of photoautotrophic Euendoliths on South African mussel beds
- Dievart, Alexia Madeleine Angèle
- Authors: Dievart, Alexia Madeleine Angèle
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435650 , vital:73176 , DOI 10.21504/10962/435650
- Description: Aphytis lingnanensis was reared and tested in South Africa in the early 2000s for augmentation against red scale on citrus and was found to be ineffective. Aphytis melinus is now commercially available and it is important that the efficacy of augmentation thereof on red scale is determined locally. Field trials, fitness assessments and molecular identification on A. melinus from two insectaries were done. Field trials was done in seven, five and six pairs of comparable release and control orchards across the Eastern and Western Cape during the seasons of 2019/2020, 2020/2021 and 2021/2022 respectively. Red scale infestation was monitored and a sample of 20 infested fruit from each orchard was randomly collected every four weeks. Aphytis spp. responsible for parasitism were identified and the percentage parasitism recorded. Results of this study of field trials suggest that the augmentation of A. melinus did not significantly increase the level of parasitism above that of the untreated control. Five repetitions with six replicates of flight and longevity tests were performed with wasps from each insectary. Wasps in the longevity test from two insectaries were kept at 23 °C and 65% RH with honey. Flight tests were performed in tubes of 16 by 30 cm, with a light above a clear, sticky ceiling at 23 °C and 65% RH. On average in five replicates, 65%, 33% and 17% A. melinus wasps were alive on day one, five and 10 respectively. The overall sex ratio was 1.58 for females to males, but 1.05, 2.19 and 2.66 for non-flyers, non-crawlers, crawlers, and flyers respectively. In flight tests for both insectaries combined, only 36.97% of wasps could initiate flight in 24 h while 56.96% remained on the tube floor, and 6.05% attempted to crawl upwards. No significant differences in flight performance were recorded between the two insectaries. Wasps from the local insectary lived significantly longer during the longevity tests but were shorter in transit than wasps from the overseas insectary. COI genes were sequenced and compared against Genbank sequences using BLAST. Molecular identifications did not confirm morphological identifications for all species, indicating unexpected genetic complexity. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
- Authors: Dievart, Alexia Madeleine Angèle
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435650 , vital:73176 , DOI 10.21504/10962/435650
- Description: Aphytis lingnanensis was reared and tested in South Africa in the early 2000s for augmentation against red scale on citrus and was found to be ineffective. Aphytis melinus is now commercially available and it is important that the efficacy of augmentation thereof on red scale is determined locally. Field trials, fitness assessments and molecular identification on A. melinus from two insectaries were done. Field trials was done in seven, five and six pairs of comparable release and control orchards across the Eastern and Western Cape during the seasons of 2019/2020, 2020/2021 and 2021/2022 respectively. Red scale infestation was monitored and a sample of 20 infested fruit from each orchard was randomly collected every four weeks. Aphytis spp. responsible for parasitism were identified and the percentage parasitism recorded. Results of this study of field trials suggest that the augmentation of A. melinus did not significantly increase the level of parasitism above that of the untreated control. Five repetitions with six replicates of flight and longevity tests were performed with wasps from each insectary. Wasps in the longevity test from two insectaries were kept at 23 °C and 65% RH with honey. Flight tests were performed in tubes of 16 by 30 cm, with a light above a clear, sticky ceiling at 23 °C and 65% RH. On average in five replicates, 65%, 33% and 17% A. melinus wasps were alive on day one, five and 10 respectively. The overall sex ratio was 1.58 for females to males, but 1.05, 2.19 and 2.66 for non-flyers, non-crawlers, crawlers, and flyers respectively. In flight tests for both insectaries combined, only 36.97% of wasps could initiate flight in 24 h while 56.96% remained on the tube floor, and 6.05% attempted to crawl upwards. No significant differences in flight performance were recorded between the two insectaries. Wasps from the local insectary lived significantly longer during the longevity tests but were shorter in transit than wasps from the overseas insectary. COI genes were sequenced and compared against Genbank sequences using BLAST. Molecular identifications did not confirm morphological identifications for all species, indicating unexpected genetic complexity. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
Plankton and macroinvertebrate dynamics in the Khakhea–Bray transboundary aquifer region
- Mungenge, Chipo Perseverance
- Authors: Mungenge, Chipo Perseverance
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435674 , vital:73178 , DOI 10.21504/10962/435674
- Description: Temporary wetlands are prevalent in semi–arid and arid climates across the globe and harbour unique faunal assemblages that significantly contribute to regional aquatic biodiversity. This study aims to enhance our understanding of the ecological dynamics of temporary wetland ecosystems, focusing on plankton dynamics, large branchiopod diversity, trophic dynamics, water quality and the impacts of freshwater salinisation in temporary pans in the Khakhea Bray Transboundary aquifer region in the North west Province, South Africa. This body of work represents field, laboratory and writing components which span the period May 2021 to December 2023. The results from this study revealed seasonal patterns in plankton diversity with a winter peak in phytoplankton diversity dominated by Zygnematophyceae species, while Chlorophyceae were dominant in summer. Zooplankton diversity was high in summer as compared to winter, with both Rotifera and Copepoda dominant in both seasons. Six large branchiopod species were found in the region, and three of these were new distribution records for the North west Province, including the first record of Phallocryptus spinosa in the salt pan that was sampled in summer. Large branchiopod diversity was mainly influenced by water temperature and phosphorous in summer, while sediment sodium influenced the diversity in winter. The stable isotope analyses used to determine trophic dynamics in these temporary pans revealed that the food web had four trophic levels, with the top predators being the notonectids Anisops sp. The dominant consumers were predatory insects such as Sigara sp., Anisops sp., Lestes sp., Rhantus sp. and Cybister sp. adults and larvae, as well as detritivorous Tomopterna sp. tadpoles. High trophic niche overlaps were found between the notonectids and the dytiscids. These temporary pans are susceptible to anthropogenic impacts, and disturbed pans were found to have elevated pH, ammonium, phosphates and dissolved oxygen compared to the undisturbed pans. A strong positive relationship was observed between chl–a and temperature, pH, dissolved oxygen, phosphates and ammonium. Chlorophyll–a concentration increased as surface area and the distance from kraals, buildings and latrines decreased. Freshwater salinisation was found to cause notable shifts in abiotic factors and benthic phytoplankton communities, favouring the proliferation of saline–tolerant diatom species at the cost of more sensitive taxa. The study also revealed that in interaction with salinisation, time also exerted a notable influence on shaping the benthic phytoplankton community. Salinity levels of 2.5 ppt and above led to significant decreases in emergent taxa richness and abundance, with Spinicaudata and Ostracoda being the most sensitive taxa to high salinities. There was a limited effect on community hatching phenology dynamics from salinity. This suggests that the main impact of salinisation in these systems will be reductions in hatching success and, hence, reduced recruitment. The study highlights the vulnerability of temporary pan ecosystems to anthropogenic influences and the complexities of interactions of organisms and the environmental conditions in these systems. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
- Authors: Mungenge, Chipo Perseverance
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435674 , vital:73178 , DOI 10.21504/10962/435674
- Description: Temporary wetlands are prevalent in semi–arid and arid climates across the globe and harbour unique faunal assemblages that significantly contribute to regional aquatic biodiversity. This study aims to enhance our understanding of the ecological dynamics of temporary wetland ecosystems, focusing on plankton dynamics, large branchiopod diversity, trophic dynamics, water quality and the impacts of freshwater salinisation in temporary pans in the Khakhea Bray Transboundary aquifer region in the North west Province, South Africa. This body of work represents field, laboratory and writing components which span the period May 2021 to December 2023. The results from this study revealed seasonal patterns in plankton diversity with a winter peak in phytoplankton diversity dominated by Zygnematophyceae species, while Chlorophyceae were dominant in summer. Zooplankton diversity was high in summer as compared to winter, with both Rotifera and Copepoda dominant in both seasons. Six large branchiopod species were found in the region, and three of these were new distribution records for the North west Province, including the first record of Phallocryptus spinosa in the salt pan that was sampled in summer. Large branchiopod diversity was mainly influenced by water temperature and phosphorous in summer, while sediment sodium influenced the diversity in winter. The stable isotope analyses used to determine trophic dynamics in these temporary pans revealed that the food web had four trophic levels, with the top predators being the notonectids Anisops sp. The dominant consumers were predatory insects such as Sigara sp., Anisops sp., Lestes sp., Rhantus sp. and Cybister sp. adults and larvae, as well as detritivorous Tomopterna sp. tadpoles. High trophic niche overlaps were found between the notonectids and the dytiscids. These temporary pans are susceptible to anthropogenic impacts, and disturbed pans were found to have elevated pH, ammonium, phosphates and dissolved oxygen compared to the undisturbed pans. A strong positive relationship was observed between chl–a and temperature, pH, dissolved oxygen, phosphates and ammonium. Chlorophyll–a concentration increased as surface area and the distance from kraals, buildings and latrines decreased. Freshwater salinisation was found to cause notable shifts in abiotic factors and benthic phytoplankton communities, favouring the proliferation of saline–tolerant diatom species at the cost of more sensitive taxa. The study also revealed that in interaction with salinisation, time also exerted a notable influence on shaping the benthic phytoplankton community. Salinity levels of 2.5 ppt and above led to significant decreases in emergent taxa richness and abundance, with Spinicaudata and Ostracoda being the most sensitive taxa to high salinities. There was a limited effect on community hatching phenology dynamics from salinity. This suggests that the main impact of salinisation in these systems will be reductions in hatching success and, hence, reduced recruitment. The study highlights the vulnerability of temporary pan ecosystems to anthropogenic influences and the complexities of interactions of organisms and the environmental conditions in these systems. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
The impacts of bottom-up and top-down drivers in shaping the herbivore community in Pafuri, Kruger National Park, South Africa
- Authors: Walker, Gareth Alexander
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435703 , vital:73180 , DOI 10.21504/10962/435703
- Description: Globally, terrestrial mammal populations are facing critical population declines and range contractions owing to habitat fragmentation and destruction, wildlife overexploitation, and climate change driven by expansion of the human population. Mammalian herbivores are integral for maintaining ecosystem structure and functionality. They do this this through herbivory, by acting as prey and cycling soil nutrients. The impacts of herbivores on ecosystems, however, vary with their spatial occupancy which is influenced by interacting bottom-up and top-down factors. Modelling the drivers of herbivore communities is no trivial task given the myriad of potential bottom-up and top-down factors, and the interactions between the two, as well as the species-specific variations in intrinsic functional traits (e.g., foraging strategy, body size, metabolic rate, etc.) influencing herbivore responses (e.g., social structure, space-use, activity patterns, etc.) to these drivers. Consequently, few studies have attempted to model both bottom-up and top-down drivers in structuring herbivore communities, particularly in an African context where predator-prey guilds include multiple species, exposed to high levels of human activity. Therefore, the overarching aim of my research was to quantify the relative effects of both bottom-up and top-down factors driving the herbivore community in the northern Pafuri region of Kruger National Park, South Africa. I utilized a combination of field (i.e., camera trap and vegetation surveys) and analytical (i.e., stable carbon isotopes from faeces and plants) techniques in conjunction with geospatial data to evaluate the impacts of bottom-up (i.e., forage quantity, quality, and water availability) and top-down (i.e., predation and anthropogenic risks) factors on herbivore spatial occupancy and activity patterns. Herbivore responses to bottom-up and top-down factors were species-specific, even among members of the same feeding guild. Specifically, I found that herbivores (varying in body size and foraging strategy) displayed temporal, spatial and in some instances, dietary shifts that reflect species-specific, ecological trade-offs between resource acquisition, and predator and human avoidance. For example, kudu (Tragelaphus strepsiceros) displayed temporal and spatial avoidance of predators and humans, and exhibited previously undocumented levels of seasonal dietary shifts which suggests that the species traded forage acquisition for reduced predation and anthropogenic risks. Further, high levels of human activity appeared to eclipse the risks associated with natural predators resulting in human induced landscapes of fear. For example, warthogs (Phacochoerus africanus) and zebra (Equus quagga) occupied habitats with higher predation risks, but displayed spatial avoidance of nature reserve boundaries which were synonymous with high levels of bushmeat poaching. Most studies focussing on the impacts of bottom-up and top-down drivers neglect to consider the roles that humans play in structuring ecological communities. The results of my thesis, however, emphasize the importance of including anthropogenic drivers when investigating the roles that various bottom-up and top-down factors play in shaping ecological communities. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
- Authors: Walker, Gareth Alexander
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435703 , vital:73180 , DOI 10.21504/10962/435703
- Description: Globally, terrestrial mammal populations are facing critical population declines and range contractions owing to habitat fragmentation and destruction, wildlife overexploitation, and climate change driven by expansion of the human population. Mammalian herbivores are integral for maintaining ecosystem structure and functionality. They do this this through herbivory, by acting as prey and cycling soil nutrients. The impacts of herbivores on ecosystems, however, vary with their spatial occupancy which is influenced by interacting bottom-up and top-down factors. Modelling the drivers of herbivore communities is no trivial task given the myriad of potential bottom-up and top-down factors, and the interactions between the two, as well as the species-specific variations in intrinsic functional traits (e.g., foraging strategy, body size, metabolic rate, etc.) influencing herbivore responses (e.g., social structure, space-use, activity patterns, etc.) to these drivers. Consequently, few studies have attempted to model both bottom-up and top-down drivers in structuring herbivore communities, particularly in an African context where predator-prey guilds include multiple species, exposed to high levels of human activity. Therefore, the overarching aim of my research was to quantify the relative effects of both bottom-up and top-down factors driving the herbivore community in the northern Pafuri region of Kruger National Park, South Africa. I utilized a combination of field (i.e., camera trap and vegetation surveys) and analytical (i.e., stable carbon isotopes from faeces and plants) techniques in conjunction with geospatial data to evaluate the impacts of bottom-up (i.e., forage quantity, quality, and water availability) and top-down (i.e., predation and anthropogenic risks) factors on herbivore spatial occupancy and activity patterns. Herbivore responses to bottom-up and top-down factors were species-specific, even among members of the same feeding guild. Specifically, I found that herbivores (varying in body size and foraging strategy) displayed temporal, spatial and in some instances, dietary shifts that reflect species-specific, ecological trade-offs between resource acquisition, and predator and human avoidance. For example, kudu (Tragelaphus strepsiceros) displayed temporal and spatial avoidance of predators and humans, and exhibited previously undocumented levels of seasonal dietary shifts which suggests that the species traded forage acquisition for reduced predation and anthropogenic risks. Further, high levels of human activity appeared to eclipse the risks associated with natural predators resulting in human induced landscapes of fear. For example, warthogs (Phacochoerus africanus) and zebra (Equus quagga) occupied habitats with higher predation risks, but displayed spatial avoidance of nature reserve boundaries which were synonymous with high levels of bushmeat poaching. Most studies focussing on the impacts of bottom-up and top-down drivers neglect to consider the roles that humans play in structuring ecological communities. The results of my thesis, however, emphasize the importance of including anthropogenic drivers when investigating the roles that various bottom-up and top-down factors play in shaping ecological communities. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
The influence of the physical environment on invertebrate larval transport, settlement and recruitment with insights on early-stage physiological performance
- Authors: Duna, Oliver Olwethu
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435663 , vital:73177 , DOI 10.21504/10962/435663
- Description: Most marine benthic organisms have a biphasic life cycle whereby the larvae are pelagic, and their distribution, transport and settlement are largely controlled by environmental factors. Among these factors, hydrodynamics have key direct effects. Nearshore water hydrodynamics are strongly influenced by wind. This study investigated whether wind indirectly affects the distribution, settlement and recruitment of invertebrate marine larvae. Additionally, larvae are exposed to a more constant, benign environment than they will experience after settlement, and therefore the possible effects of larval thermal history on the physiological plasticity of settled organisms was also estimated for possible long-term insights into population dynamics. Four sites, two on the western and two on the eastern side of Algoa Bay in Gqeberha (formerly known as Port Elizabeth), South Africa, were chosen for larval collection. Sites at opposite ends of the bay were selected as they were towards either side of the dominant wind directions in this region. At each site, two sampling stations were identified at 300 and 900 metres offshore, with station replicates 300 metres apart. Sampling of marine invertebrate larvae was conducted after two days of westerly or easterly winds at either of the two western or eastern sites. The number of hours of unidirectional wind determined the prevalent wind on the two days prior to sampling. Samples were collected with a plankton pump at three depths; surface, mid-depth and bottom. Simultaneously with plankton sampling, water properties (fluorescence, temperature, zonal and meridional flow, salinity and oxygen) were measured. Settlement/recruitment samples of mussels and barnacles were collected at six sites, two at either edge of the bay and two within the center of the bay. Plastic scouring pads and PVC plastic plates covered with safety-walk, placed at least 30 cm from each other, were used to collect settlers/recruits of mussels and barnacles, respectively. The pads and plates were replaced on a monthly basis for 14 months to quantify monthly settlement/recruitment. At four of the six sites used for quantifying settlement/recruitment, settlers and recruits were collected to measure the physiological responses of these two ontogenetic stages (settlers and recruits) to acute temperature variations which they might experience in the intertidal zone. Three temperature loggers were used to record real time temperature variation at each site over the period (one month) in which the artificial collectors were in position. Factorial ANOVA was used to investigate the effects of wind, depth, site, and distance from the shore on the abundance of larvae in the water column, as well as the effects of month and site on settlement/recruitment. Additionally, multiple regression analyses were used to investigate the effects of physical parameters, including upwelling and dissipation of turbulent kinetic energy, on the abundance of both larvae, settlers and recruits. Distance-based linear models and redundancy analyses were also carried out on the abundances of invertebrate larvae. Correlation analyses were performed to investigate the effect of the number of hours of directional wind on settlement. Lastly, correlation analyses between wind and currents were carried out. The results revealed a link between winds and currents, with wind speed directly proportional to surface current speed, which, as expected, decreased with depth. Current direction was, however, not perfectly aligned with wind direction. Larval distribution, settlement and recruitment were largely associated with the nearshore dissipation of turbulent kinetic energy and upwelling. Thus, overall larval, settler and recruit abundances were indirectly affected by wind. Physiologically, there was no significant difference in oxygen consumption between the juvenile ontogenetic stages (settlers and recruits) of mussels. Recruits exposed to average and maximum temperatures, however, consumed more oxygen than those exposed to low temperatures. Wind and wind-mediated currents as well as temperature have been shown to shape the distribution and shore supply of larvae, and this study contributes to the broad knowledge of population dynamics and replenishment, and the tight relation of abiotic factors affecting biological processes on the shore. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
- Authors: Duna, Oliver Olwethu
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435663 , vital:73177 , DOI 10.21504/10962/435663
- Description: Most marine benthic organisms have a biphasic life cycle whereby the larvae are pelagic, and their distribution, transport and settlement are largely controlled by environmental factors. Among these factors, hydrodynamics have key direct effects. Nearshore water hydrodynamics are strongly influenced by wind. This study investigated whether wind indirectly affects the distribution, settlement and recruitment of invertebrate marine larvae. Additionally, larvae are exposed to a more constant, benign environment than they will experience after settlement, and therefore the possible effects of larval thermal history on the physiological plasticity of settled organisms was also estimated for possible long-term insights into population dynamics. Four sites, two on the western and two on the eastern side of Algoa Bay in Gqeberha (formerly known as Port Elizabeth), South Africa, were chosen for larval collection. Sites at opposite ends of the bay were selected as they were towards either side of the dominant wind directions in this region. At each site, two sampling stations were identified at 300 and 900 metres offshore, with station replicates 300 metres apart. Sampling of marine invertebrate larvae was conducted after two days of westerly or easterly winds at either of the two western or eastern sites. The number of hours of unidirectional wind determined the prevalent wind on the two days prior to sampling. Samples were collected with a plankton pump at three depths; surface, mid-depth and bottom. Simultaneously with plankton sampling, water properties (fluorescence, temperature, zonal and meridional flow, salinity and oxygen) were measured. Settlement/recruitment samples of mussels and barnacles were collected at six sites, two at either edge of the bay and two within the center of the bay. Plastic scouring pads and PVC plastic plates covered with safety-walk, placed at least 30 cm from each other, were used to collect settlers/recruits of mussels and barnacles, respectively. The pads and plates were replaced on a monthly basis for 14 months to quantify monthly settlement/recruitment. At four of the six sites used for quantifying settlement/recruitment, settlers and recruits were collected to measure the physiological responses of these two ontogenetic stages (settlers and recruits) to acute temperature variations which they might experience in the intertidal zone. Three temperature loggers were used to record real time temperature variation at each site over the period (one month) in which the artificial collectors were in position. Factorial ANOVA was used to investigate the effects of wind, depth, site, and distance from the shore on the abundance of larvae in the water column, as well as the effects of month and site on settlement/recruitment. Additionally, multiple regression analyses were used to investigate the effects of physical parameters, including upwelling and dissipation of turbulent kinetic energy, on the abundance of both larvae, settlers and recruits. Distance-based linear models and redundancy analyses were also carried out on the abundances of invertebrate larvae. Correlation analyses were performed to investigate the effect of the number of hours of directional wind on settlement. Lastly, correlation analyses between wind and currents were carried out. The results revealed a link between winds and currents, with wind speed directly proportional to surface current speed, which, as expected, decreased with depth. Current direction was, however, not perfectly aligned with wind direction. Larval distribution, settlement and recruitment were largely associated with the nearshore dissipation of turbulent kinetic energy and upwelling. Thus, overall larval, settler and recruit abundances were indirectly affected by wind. Physiologically, there was no significant difference in oxygen consumption between the juvenile ontogenetic stages (settlers and recruits) of mussels. Recruits exposed to average and maximum temperatures, however, consumed more oxygen than those exposed to low temperatures. Wind and wind-mediated currents as well as temperature have been shown to shape the distribution and shore supply of larvae, and this study contributes to the broad knowledge of population dynamics and replenishment, and the tight relation of abiotic factors affecting biological processes on the shore. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
Developing a community of practice to promote the use of biological control in the integrated management of Prosopis in South Africa
- Authors: Van Staden, Gretha
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434998 , vital:73122
- Description: Prosopis spp. are non-native species present in the arid parts of southern Africa. These trees originated in the Americas and were first introduced as a source of fodder, shade, and wood, but have become invasive, negatively impacting local biodiversity, and disrupting the delivery of ecosystem services. Some species of Prosopis hybridise freely, complicating identification and subsequent control. The control of Prosopis in the Northern Cape Province of South Africa is still contentious, because of the perceived benefits to some landowners. The effectiveness of biological control agents to control the spread of Prosopis has been quantified, more damaging agents have been considered in recent years because of the continued increase in Prosopis density. Research into the establishment of a Community of Practice (CoP) to address the differences in perceptions regarding the control of Prosopis in the Northern Cape Province is considered an appropriate starting point to conceptualise the challenges to the successful integrated management of Prosopis. As farmers are the main stakeholders involved with Prosopis control, understanding the role of farmers and the functioning of farming enterprises in the Northern Cape is imperative for the development of both a CoP as well as the appropriate management of Prosopis. To foster engagement with as many stakeholders as possible, workshops dealing with biological control as part on an integrated approach to the control of Prosopis were held in towns in the Northern Cape Province: Groblershoop, Brandvlei, Kenhardt, Upington, Prieska and Williston. Some of the main concerns of the landusers include the host specificity of the released biocontrol agents, as well as increased transparency and communication. This is especially in regards to the research on present and future biological control agents that will possibly improve the perceptions of stakeholders. The mechanical harvesting and use of biomass as a control method was supported, especially where farmers were removing Prosopis from their properties, leaving large amounts of biomass in the veld. Continued research into Prosopis in the Northern Cape needs to consider the possibility of secondary invasions, especially in areas where native bush encroachment is already a concern. The impact of clearing in terms of natural grazing and animal improvement needs to be quantified, to conceptualise the importance of maintaining better veld quality versus using Prosopis for fodder. Biological control targeting not only the reproductive output of the trees, but also the standing biomass has support from the majority of the land users in the Northern Cape Province following these workshops and this study shows the importance of a socio-ecological approach to the control of landscape scale invasion. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Van Staden, Gretha
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434998 , vital:73122
- Description: Prosopis spp. are non-native species present in the arid parts of southern Africa. These trees originated in the Americas and were first introduced as a source of fodder, shade, and wood, but have become invasive, negatively impacting local biodiversity, and disrupting the delivery of ecosystem services. Some species of Prosopis hybridise freely, complicating identification and subsequent control. The control of Prosopis in the Northern Cape Province of South Africa is still contentious, because of the perceived benefits to some landowners. The effectiveness of biological control agents to control the spread of Prosopis has been quantified, more damaging agents have been considered in recent years because of the continued increase in Prosopis density. Research into the establishment of a Community of Practice (CoP) to address the differences in perceptions regarding the control of Prosopis in the Northern Cape Province is considered an appropriate starting point to conceptualise the challenges to the successful integrated management of Prosopis. As farmers are the main stakeholders involved with Prosopis control, understanding the role of farmers and the functioning of farming enterprises in the Northern Cape is imperative for the development of both a CoP as well as the appropriate management of Prosopis. To foster engagement with as many stakeholders as possible, workshops dealing with biological control as part on an integrated approach to the control of Prosopis were held in towns in the Northern Cape Province: Groblershoop, Brandvlei, Kenhardt, Upington, Prieska and Williston. Some of the main concerns of the landusers include the host specificity of the released biocontrol agents, as well as increased transparency and communication. This is especially in regards to the research on present and future biological control agents that will possibly improve the perceptions of stakeholders. The mechanical harvesting and use of biomass as a control method was supported, especially where farmers were removing Prosopis from their properties, leaving large amounts of biomass in the veld. Continued research into Prosopis in the Northern Cape needs to consider the possibility of secondary invasions, especially in areas where native bush encroachment is already a concern. The impact of clearing in terms of natural grazing and animal improvement needs to be quantified, to conceptualise the importance of maintaining better veld quality versus using Prosopis for fodder. Biological control targeting not only the reproductive output of the trees, but also the standing biomass has support from the majority of the land users in the Northern Cape Province following these workshops and this study shows the importance of a socio-ecological approach to the control of landscape scale invasion. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
Functional significance of mixed-species groups for zebra (Equus quagga) in savanna habitats
- Corry-Roberts, Carys Alexandra
- Authors: Corry-Roberts, Carys Alexandra
- Date: 2024-04-04
- Subjects: Herding behavior in animals , Zebras Food , Foraging , Zebras Behavior , Zebras Conservation , Herbivores Habitat
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434925 , vital:73116
- Description: Globally, herbivore populations are facing decline, which threatens their crucial role within the ecosystems they inhabit. Herbivores are essential to ecosystem structure and function because they act as prey for higher trophic levels, cycle soil nutrients, and alter vegetation structure. Understanding the drivers that shape herbivore habitat use and selection are therefore critical for making informed conservation and management decisions. Like all prey species, herbivores must balance a trade-off between acquiring resources and avoiding predation which is influenced by a number of species-specific intrinsic traits and various ecological drivers. Herbivores can also track risk over space and time, and certain habitat structures and landscape features can influence risk perception; this is termed the landscape of fear. To mitigate the risk-reward trade-off, herbivores employ a suite of behavioural responses. Group formation is likely the most well-documented of these responses. However, grouping results in the information-competition trade-off, wherein group members may obtain valuable information regarding the location of resources and/or the risk of predation, but may suffer from increased competition, especially among larger group sizes. Mixed-species groups—an assemblage of two or more species in close spatial association—have the potential to mitigate some of the competitive costs of larger conspecific groups and enhance the anti-predator and/or foraging benefits of grouping. African savannas provide a particularly useful natural laboratory to study mixed-species groups, due to the high diversity of prey species and habitat heterogeneity present. Previous studies have investigated the mixed-species effect within these systems, but have yet to quantify some of the identity-dependent costs and benefits associated with mixed-species herding. Thus, the overall aim of my study was to quantify the functional significance of mixed-species groups for zebra (Equus quagga) herding with two of their most common herding partners, impala (Aepyceros melampus) and wildebeest (Connochaetes taurinus). My study was conducted at multiple spatial scales in the southern portion of the Kruger National Park, South Africa between 2021 and 2022. I found that mixed-species herding altered zebra landscape use, resulting in a quantifiable intensification of habitat use compared to when they herd with conspecifics. I did not observe significant habitat expansion for zebra in mixed-species herds compared to single-species herds, likely because the anti-predator benefits of mixed-species herding convey enhanced foraging opportunities in already-safe habitats. Zebra foraging with impala achieved greater foraging success and experienced lower levels of competition, compared to zebra foraging with wildebeest. However, compared to foraging with conspecifics, the levelled landscape of fear zebra experienced when foraging with wildebeest resulted in increased foraging success across the landscape. My findings highlight the context-dependency of the costs and benefits associated with different herding partners for zebra, and suggest that in mixed-species herds with wildebeest, zebra experience increased potential fitness benefits compared to herding with impala or conspecifics. Overall, the findings of my study suggest that mixed-species herding is an important tool in the suite of anti-predator behaviours employed by prey species, and one with significant implications for survivorship, habitat use, and ultimately, community level interactions. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Corry-Roberts, Carys Alexandra
- Date: 2024-04-04
- Subjects: Herding behavior in animals , Zebras Food , Foraging , Zebras Behavior , Zebras Conservation , Herbivores Habitat
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434925 , vital:73116
- Description: Globally, herbivore populations are facing decline, which threatens their crucial role within the ecosystems they inhabit. Herbivores are essential to ecosystem structure and function because they act as prey for higher trophic levels, cycle soil nutrients, and alter vegetation structure. Understanding the drivers that shape herbivore habitat use and selection are therefore critical for making informed conservation and management decisions. Like all prey species, herbivores must balance a trade-off between acquiring resources and avoiding predation which is influenced by a number of species-specific intrinsic traits and various ecological drivers. Herbivores can also track risk over space and time, and certain habitat structures and landscape features can influence risk perception; this is termed the landscape of fear. To mitigate the risk-reward trade-off, herbivores employ a suite of behavioural responses. Group formation is likely the most well-documented of these responses. However, grouping results in the information-competition trade-off, wherein group members may obtain valuable information regarding the location of resources and/or the risk of predation, but may suffer from increased competition, especially among larger group sizes. Mixed-species groups—an assemblage of two or more species in close spatial association—have the potential to mitigate some of the competitive costs of larger conspecific groups and enhance the anti-predator and/or foraging benefits of grouping. African savannas provide a particularly useful natural laboratory to study mixed-species groups, due to the high diversity of prey species and habitat heterogeneity present. Previous studies have investigated the mixed-species effect within these systems, but have yet to quantify some of the identity-dependent costs and benefits associated with mixed-species herding. Thus, the overall aim of my study was to quantify the functional significance of mixed-species groups for zebra (Equus quagga) herding with two of their most common herding partners, impala (Aepyceros melampus) and wildebeest (Connochaetes taurinus). My study was conducted at multiple spatial scales in the southern portion of the Kruger National Park, South Africa between 2021 and 2022. I found that mixed-species herding altered zebra landscape use, resulting in a quantifiable intensification of habitat use compared to when they herd with conspecifics. I did not observe significant habitat expansion for zebra in mixed-species herds compared to single-species herds, likely because the anti-predator benefits of mixed-species herding convey enhanced foraging opportunities in already-safe habitats. Zebra foraging with impala achieved greater foraging success and experienced lower levels of competition, compared to zebra foraging with wildebeest. However, compared to foraging with conspecifics, the levelled landscape of fear zebra experienced when foraging with wildebeest resulted in increased foraging success across the landscape. My findings highlight the context-dependency of the costs and benefits associated with different herding partners for zebra, and suggest that in mixed-species herds with wildebeest, zebra experience increased potential fitness benefits compared to herding with impala or conspecifics. Overall, the findings of my study suggest that mixed-species herding is an important tool in the suite of anti-predator behaviours employed by prey species, and one with significant implications for survivorship, habitat use, and ultimately, community level interactions. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
High-altitude temporary systems and macroinvertebrate composition in La Nina cycle, Maloti-Drakensberg
- Authors: Moyo, Sibusisiwe
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434963 , vital:73119
- Description: The Maloti-Drakensberg Mountain is an important water catchment area for South Africa and Lesotho. It is rich in biodiversity in terms of endemic flora and fauna and has a high density of temporary wetlands in the form of Afromontane tarns and rock pools. There is, however, limited information on the distribution of macroinvertebrates in these ecosystems and environmental factors that drives their composition. To that end, this study investigated macroinvertebrate diversity and the effect of environmental factors on community assemblage in the Maloti-Drakensberg Mountain temporary wetlands, specifically tarns and rock pools. The main aims were to describe the abundance, diversity, and distribution of macroinvertebrates in temporary wetlands in the Maloti-Drakensberg Mountain. Furthermore, it aimed to investigate the effect of environmental variables on habitat structuring, spatial variations, and macroinvertebrate community composition in tarns and rockpools in the Maloti-Drakensberg Mountain. Seventy temporary wetlands (29 tarns and 41 rock pools) were surveyed over a once-off sampling (3 March – 7 April 2022). The wetlands were from 3 provinces including Eastern Cape, Free State, and KwaZulu-Natal. 15278 individual macroinvertebrates from 13 orders, 35 families, and 46 genera were collected and identified. Species accumulation curves incorporating both tarns and rock pools showed that observed results were significantly lower than Chao 1 and Chao 2 estimates meaning that the sampled sites were not enough to provide a comprehensive picture of the biodiversity in the region. This was also observed for rock pools when the temporary wetlands were analysed separately, however, the Chao 1 and Chao 2 values for tarns were almost equal to the observed values implying that sampled sites were enough to give a detailed picture of macroinvertebrate composition in tarns. Tarns and rock pools were significantly different in terms of species richness and Shannon-Wiener diversity index but showed no significant differences for relative abundance and Pielou’s evenness. Of the 35 families identified, 19 were considered core taxa with Dytiscidae and Corixidae being the most dominant. Additionally, there was moderate Nestedness metric based on Overlap and Decreasing Fill (NODF) value of 33.05369, with 32 out of 35 families being idiosyncratic. Predators were the dominant feeding group with omnivores being the least common. Non-Metric multi-Dimensional Scaling (NMDS) and PERMANOVA results showed that tarns and rock pools supported distinctly different macroinvertebrate communities per wetland type and location respectively. Physicochemically, tarns and rock pools were significantly different in terms of water temperature, depth, area, and chlorophyll-a concentration with tarns exhibiting higher values. Temporary wetlands in the sampled localities differed in terms of pH, EC, DO, water temperature, altitude, and phosphate. Principal Component Analysis showed that tarns were positively associated with area and depth whereas rock pools with phosphate. Akaike’s Information Criterion (AICc) selection showed that area, pH, and water temperature were significantly influencing community assemblage in temporary wetlands. Furthermore, area significantly influenced all four measured biodiversity indices: relative abundance, species richness, Pielou’s evenness and Shannon-Wiener Index, with water temperature also influencing relative abundance and altitude, species richness. Overall, the species composition showed that the sites were sampled later in the hydroperiod as evidenced by the dominance of active dispersers. The extended distribution of Prosthetops gladiator in the Eastern Cape highlights the need for extensive surveys to provide valuable insight into the distribution patterns of macroinvertebrates in the region. Furthermore, the interplay between physicochemical variables and biodiversity indices emphasized area, water temperature and pH as significant factors. However, their limited explanatory power suggests that obscure factors also influence the diversity cementing the need for further exploration of these enigmatic systems. Taxonomic challenges, especially regarding large branchiopods, hindered precise species distribution and conservation status determination. This study is the first to provide a comprehensive list of macroinvertebrates from temporary wetlands in the Maloti-Drakensberg Mountain. These results establish a valuable baseline for future biological monitoring studies, particularly in assessing species composition and responses to anthropogenic activities. It is a timeous study considering the sensitivity of these systems to climate change as they act as sentinel sites hence the need for foundational data on which to make future projections. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Moyo, Sibusisiwe
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434963 , vital:73119
- Description: The Maloti-Drakensberg Mountain is an important water catchment area for South Africa and Lesotho. It is rich in biodiversity in terms of endemic flora and fauna and has a high density of temporary wetlands in the form of Afromontane tarns and rock pools. There is, however, limited information on the distribution of macroinvertebrates in these ecosystems and environmental factors that drives their composition. To that end, this study investigated macroinvertebrate diversity and the effect of environmental factors on community assemblage in the Maloti-Drakensberg Mountain temporary wetlands, specifically tarns and rock pools. The main aims were to describe the abundance, diversity, and distribution of macroinvertebrates in temporary wetlands in the Maloti-Drakensberg Mountain. Furthermore, it aimed to investigate the effect of environmental variables on habitat structuring, spatial variations, and macroinvertebrate community composition in tarns and rockpools in the Maloti-Drakensberg Mountain. Seventy temporary wetlands (29 tarns and 41 rock pools) were surveyed over a once-off sampling (3 March – 7 April 2022). The wetlands were from 3 provinces including Eastern Cape, Free State, and KwaZulu-Natal. 15278 individual macroinvertebrates from 13 orders, 35 families, and 46 genera were collected and identified. Species accumulation curves incorporating both tarns and rock pools showed that observed results were significantly lower than Chao 1 and Chao 2 estimates meaning that the sampled sites were not enough to provide a comprehensive picture of the biodiversity in the region. This was also observed for rock pools when the temporary wetlands were analysed separately, however, the Chao 1 and Chao 2 values for tarns were almost equal to the observed values implying that sampled sites were enough to give a detailed picture of macroinvertebrate composition in tarns. Tarns and rock pools were significantly different in terms of species richness and Shannon-Wiener diversity index but showed no significant differences for relative abundance and Pielou’s evenness. Of the 35 families identified, 19 were considered core taxa with Dytiscidae and Corixidae being the most dominant. Additionally, there was moderate Nestedness metric based on Overlap and Decreasing Fill (NODF) value of 33.05369, with 32 out of 35 families being idiosyncratic. Predators were the dominant feeding group with omnivores being the least common. Non-Metric multi-Dimensional Scaling (NMDS) and PERMANOVA results showed that tarns and rock pools supported distinctly different macroinvertebrate communities per wetland type and location respectively. Physicochemically, tarns and rock pools were significantly different in terms of water temperature, depth, area, and chlorophyll-a concentration with tarns exhibiting higher values. Temporary wetlands in the sampled localities differed in terms of pH, EC, DO, water temperature, altitude, and phosphate. Principal Component Analysis showed that tarns were positively associated with area and depth whereas rock pools with phosphate. Akaike’s Information Criterion (AICc) selection showed that area, pH, and water temperature were significantly influencing community assemblage in temporary wetlands. Furthermore, area significantly influenced all four measured biodiversity indices: relative abundance, species richness, Pielou’s evenness and Shannon-Wiener Index, with water temperature also influencing relative abundance and altitude, species richness. Overall, the species composition showed that the sites were sampled later in the hydroperiod as evidenced by the dominance of active dispersers. The extended distribution of Prosthetops gladiator in the Eastern Cape highlights the need for extensive surveys to provide valuable insight into the distribution patterns of macroinvertebrates in the region. Furthermore, the interplay between physicochemical variables and biodiversity indices emphasized area, water temperature and pH as significant factors. However, their limited explanatory power suggests that obscure factors also influence the diversity cementing the need for further exploration of these enigmatic systems. Taxonomic challenges, especially regarding large branchiopods, hindered precise species distribution and conservation status determination. This study is the first to provide a comprehensive list of macroinvertebrates from temporary wetlands in the Maloti-Drakensberg Mountain. These results establish a valuable baseline for future biological monitoring studies, particularly in assessing species composition and responses to anthropogenic activities. It is a timeous study considering the sensitivity of these systems to climate change as they act as sentinel sites hence the need for foundational data on which to make future projections. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
Managing releases of Anagyrus vladimiri (Triapitsyn) to augment biocontrol of the citrus mealybug Planococcus citri (Risso) in South African citrus orchards
- Authors: Mommsen, Wayne Trevor
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434952 , vital:73118
- Description: In May 2019, South Korean inspectors rejected numerous grapefruit consignments from Letsitele, Hoedspruit and Onderberg in South Arica, because of live mealybug found on fruit. Growers expressed deep concern as mealybug management to a phytosanitary level was almost unattainable. Regular spray interventions for control of citrus black spot fungus, Phyllosticta citricarpa, and citrus thrips, Scirtothrips aurantii, cause repercussions in mealybug populations because they undermine the naturally occurring biocontrol complex. As part of an Integrated Pest Management (IPM) strategy, release of commercially produced parasitoids is common practice, to augment the naturally occurring beneficial insect populations. Prior knowledge of the harmful effects of insecticides on parasitoids is essential to IPM planning and the success of the biocontrol component in such a programme. Timing of augmentative releases to coincide with the phenology of citrus and the mealybug pest is also considered important for the successful establishment and control. Consequently, field trials were conducted to compare efficacy of early vs. late releases of Anagyrus vladimiri (Triapitsyn), an effective parasitoid of the citrus mealybug, Planococcus citri (Risso). Semi-field bioassays were conducted concurrently to determine the impact of various thripicides on A. vladimiri. The impact of sulfoxaflor, spinetoram, spirotetramat and prothiofos were rated harmless, as A. vladimiri mortality was lower than 25% after coming into contact with aged residues between 7 and 14 days old. October and November releases of A. vladimiri resulted in early parasitism and lowered peak-infestation of mealybug. January releases are possibly too late in grapefruit and lemon, open field, orchards, considering parasitism by A. vladimiri peaked in February. In mandarin orchards under net, percentage parasitism of 3rd instar mealybug increased a month later. Notably, at harvest, the difference in efficacy between treatments was not clear. This could be explained by high levels of natural parasitism observed in the treated and untreated orchards, which emphasises the importance of conservation biocontrol. In a second season, the proportion of hyperparasitoids captured (61%) from samples of mealybug-infested fruit was larger than the proportion of primary parasitoids, Anagyrus vladimiri, Coccidoxenoides perminutus (Girault) and Leptomastix dactylopii (Howard) (39%), which was far lower than the captures of eclosing primary parasitoids the previous season, which was 60%. The new discovery of Pseudaphycus sp. in citrus orchards in South Africa could be a key in explaining the uncontrollable levels of mealybug experienced and has drawn attention to a need for further understanding of ecological factors that influence biological control in citrus. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Mommsen, Wayne Trevor
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434952 , vital:73118
- Description: In May 2019, South Korean inspectors rejected numerous grapefruit consignments from Letsitele, Hoedspruit and Onderberg in South Arica, because of live mealybug found on fruit. Growers expressed deep concern as mealybug management to a phytosanitary level was almost unattainable. Regular spray interventions for control of citrus black spot fungus, Phyllosticta citricarpa, and citrus thrips, Scirtothrips aurantii, cause repercussions in mealybug populations because they undermine the naturally occurring biocontrol complex. As part of an Integrated Pest Management (IPM) strategy, release of commercially produced parasitoids is common practice, to augment the naturally occurring beneficial insect populations. Prior knowledge of the harmful effects of insecticides on parasitoids is essential to IPM planning and the success of the biocontrol component in such a programme. Timing of augmentative releases to coincide with the phenology of citrus and the mealybug pest is also considered important for the successful establishment and control. Consequently, field trials were conducted to compare efficacy of early vs. late releases of Anagyrus vladimiri (Triapitsyn), an effective parasitoid of the citrus mealybug, Planococcus citri (Risso). Semi-field bioassays were conducted concurrently to determine the impact of various thripicides on A. vladimiri. The impact of sulfoxaflor, spinetoram, spirotetramat and prothiofos were rated harmless, as A. vladimiri mortality was lower than 25% after coming into contact with aged residues between 7 and 14 days old. October and November releases of A. vladimiri resulted in early parasitism and lowered peak-infestation of mealybug. January releases are possibly too late in grapefruit and lemon, open field, orchards, considering parasitism by A. vladimiri peaked in February. In mandarin orchards under net, percentage parasitism of 3rd instar mealybug increased a month later. Notably, at harvest, the difference in efficacy between treatments was not clear. This could be explained by high levels of natural parasitism observed in the treated and untreated orchards, which emphasises the importance of conservation biocontrol. In a second season, the proportion of hyperparasitoids captured (61%) from samples of mealybug-infested fruit was larger than the proportion of primary parasitoids, Anagyrus vladimiri, Coccidoxenoides perminutus (Girault) and Leptomastix dactylopii (Howard) (39%), which was far lower than the captures of eclosing primary parasitoids the previous season, which was 60%. The new discovery of Pseudaphycus sp. in citrus orchards in South Africa could be a key in explaining the uncontrollable levels of mealybug experienced and has drawn attention to a need for further understanding of ecological factors that influence biological control in citrus. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
The use of gabions as a tool for ecological engineering
- Authors: Seath, Jessica Lauren
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434987 , vital:73121
- Description: Anthropogenic activities are centred in coastal ecosystems, including the development of harbours and/or marinas. The artificial structures used in coastal development typically has a different composition, orientation and level of complexity to that of natural ecosystems contributing to loss of biodiversity and increased incidence of invasive species. Ecological engineering research is attempting to identify different types of structures and materials that can increase species diversity and target species of conservation concern in coastal systems. The aim of this study was to investigate the efficacy of gabions (rock filled structures) as an ecological engineering tool by comparing community structure on these structures with pre-existing seawall structures within a small harbour and marina in South Africa (Knysna Harbour). The objectives of the study were to compare the differences in; 1) fish and; 2) colonising organisms’ diversity and composition between two artificial structures. Thirteen gabion boxes were deployed in Knysna Harbour and together with corresponding seawalls, monitored quarterly over a period of 12 months (August 2020 – August 2021) to assess taxon and functional richness, diversity, abundance and composition of fish, invertebrate and algal species. Physico-chemical characteristics of the water body were also monitored quarterly. Remote underwater video systems were used to quantify MaxN (maximum number of a fish species in the frame at any one time during each set that gives an indication of relative abundance) and identify fish species. The results of the two-way crossed ANOVAs indicated that gabion habitats recruited greater numbers and more types of fish species and from more functional groups than the seawalls, especially omnivorous and carnivorous fish. Additionally, photoquadrats were used to quantify percentage cover, counts and to identify colonising taxa. The results of the two-way crossed ANOVAs indicated that gabions hosted greater numbers of species resulting in a higher overall diversity and abundance of colonising organisms than seawalls. By contrast, the seawalls supported more types of functional groups of colonising organisms than gabions, largely due to abundances of different algal species. The results from the crossed PERMANOVAs indicated that the composition of fish and colonising organisms were vastly different from one another, and that each habitat was supporting very different functional groups. Results indicate that whilst both gabions and seawalls contain several alien species, that the ratio of native to alien species is higher in gabion habitats. Additionally, this research observed that gabion structures hosted four species listed on the IUCN Red List of Threatened Species. This study has highlighted that the use of gabions (with their natural increased complexity) could be important to consider for the future of urban coastal development in harbours such as in Knysna Harbour. Ecological engineering projects using gabions have the potential to be used in South Africa in projects that aim at increasing biodiversity in urban coastal environments. As well as increasing the settlement and abundance of habitat-forming ecosystem engineers to ensure the long-term stability of these ecosystems. They can be used both in the development of new costal development projects as well as in an ad-hoc fashion where they can be interspersed on seawalls in harbours. Additionally, gabions have the potential to be used in projects that target species of conservation concern such as the endangered Knysna Seahorse (Hippocampus capensis). It is, however, important to monitor the invasion by non-native species in future ecological engineering projects in South Africa as well as their potential for creating ecological traps (a situation in which an organism may be convinced to settle in a low-quality habitat) for certain species. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
- Authors: Seath, Jessica Lauren
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434987 , vital:73121
- Description: Anthropogenic activities are centred in coastal ecosystems, including the development of harbours and/or marinas. The artificial structures used in coastal development typically has a different composition, orientation and level of complexity to that of natural ecosystems contributing to loss of biodiversity and increased incidence of invasive species. Ecological engineering research is attempting to identify different types of structures and materials that can increase species diversity and target species of conservation concern in coastal systems. The aim of this study was to investigate the efficacy of gabions (rock filled structures) as an ecological engineering tool by comparing community structure on these structures with pre-existing seawall structures within a small harbour and marina in South Africa (Knysna Harbour). The objectives of the study were to compare the differences in; 1) fish and; 2) colonising organisms’ diversity and composition between two artificial structures. Thirteen gabion boxes were deployed in Knysna Harbour and together with corresponding seawalls, monitored quarterly over a period of 12 months (August 2020 – August 2021) to assess taxon and functional richness, diversity, abundance and composition of fish, invertebrate and algal species. Physico-chemical characteristics of the water body were also monitored quarterly. Remote underwater video systems were used to quantify MaxN (maximum number of a fish species in the frame at any one time during each set that gives an indication of relative abundance) and identify fish species. The results of the two-way crossed ANOVAs indicated that gabion habitats recruited greater numbers and more types of fish species and from more functional groups than the seawalls, especially omnivorous and carnivorous fish. Additionally, photoquadrats were used to quantify percentage cover, counts and to identify colonising taxa. The results of the two-way crossed ANOVAs indicated that gabions hosted greater numbers of species resulting in a higher overall diversity and abundance of colonising organisms than seawalls. By contrast, the seawalls supported more types of functional groups of colonising organisms than gabions, largely due to abundances of different algal species. The results from the crossed PERMANOVAs indicated that the composition of fish and colonising organisms were vastly different from one another, and that each habitat was supporting very different functional groups. Results indicate that whilst both gabions and seawalls contain several alien species, that the ratio of native to alien species is higher in gabion habitats. Additionally, this research observed that gabion structures hosted four species listed on the IUCN Red List of Threatened Species. This study has highlighted that the use of gabions (with their natural increased complexity) could be important to consider for the future of urban coastal development in harbours such as in Knysna Harbour. Ecological engineering projects using gabions have the potential to be used in South Africa in projects that aim at increasing biodiversity in urban coastal environments. As well as increasing the settlement and abundance of habitat-forming ecosystem engineers to ensure the long-term stability of these ecosystems. They can be used both in the development of new costal development projects as well as in an ad-hoc fashion where they can be interspersed on seawalls in harbours. Additionally, gabions have the potential to be used in projects that target species of conservation concern such as the endangered Knysna Seahorse (Hippocampus capensis). It is, however, important to monitor the invasion by non-native species in future ecological engineering projects in South Africa as well as their potential for creating ecological traps (a situation in which an organism may be convinced to settle in a low-quality habitat) for certain species. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-04
A molecular investigation of stem-galling Tetramesa Walker (Hymenoptera: Eurytomidae) on African grasses: applications to biological control
- Van Steenderen, Clarke Julian Mignon
- Authors: Van Steenderen, Clarke Julian Mignon
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432565 , vital:72881 , DOI 10.21504/10962/432565
- Description: South Africa is a larger donor than receiver of alien grasses, where approximately 15% (_ 165 spp.) of the country's native grass species have become naturalised elsewhere. Many of these grasses have become serious invaders, causing significant damage to native species, habitat structure, and ecosystem functioning. Biological control is a sustainable and cost-effective method for the control of invasive weeds, but its application to invasive grasses has been approached with trepidation in the past due to the fears of a lack of host-specific herbivores that may cause non-target damage to agriculturally-important crops. The Tetramesa Walker genus (Hymenoptera: Eurytomidae) is one of three genera in the family that feed exclusively on grasses, and have a record of being host-specific to a particular species, or complex of closely-related congeners. There are over 200 described Tetramesa species, but this taxonomic effort has occurred almost exclusively in the Northern Hemisphere. Only about 2% of the described species are from Africa, with none from southern Africa despite the high diversity of grasses in the region. The low morphological variability between many Tetramesa groups has made identification difficult, where there may in fact be multiple undiscovered cryptic species. This thesis generated genetic sequence data (mitochondrial COI and nuclear 28S) that revealed at least eight native southern African Tetramesa taxa that are new to science, focusing particularly on the assemblages associated with Eragrostis curvula Nees (African lovegrass) and Sporobolus pyramidalis Beauv. and S. natalensis Steud. (giant rat's tail grass) which are alien invasive pests in Australia. Approximately 200 eurytomid wasps were collected and sequenced from 19 grass species across six South African provinces. Additionally, 27 grass species were sequenced using four gene regions (rps16-trnK, rps16, rpl32-trnL, and ITS), which were added to existing sequence data to build a dataset comprising over 700 sequences. Field host ranges and the use of host grass genetic proxies were important in making inferences about the host-specificity of eurytomid wasps of interest. Nine Tetramesa groups appeared to be host-specific to a single grass species, while six Tetramesa were associated with multiple species in a single grass genus. Since S. pyramidalis, S. natalensis, S. africanus, Hyparrhenia hirta, E. trichophora, and Andropogon gayanus are weeds elsewhere, there are at least six potential Tetramesa biological control agents that have been identified. A high diversity of Tetramesa on grasses within the Eragrostis genus was reported, with at least four taxa associated with E. curvula. It is currently uncertain whether these taxa represent different cryptic species or intraspecific populations that are the result of geographic sub-structuring. No-choice host-specificity testing using Tetramesa sp. 4 on E. curvula revealed that the wasp could complete its lifecycle on two non-target African grasses; namely E. plana and E. planiculmis. The wasp was also recorded on other Eragrostis species in the field (namely E. biflora and E. capensis). Using grass genetic sequences obtained in this study, it was found that there are four native Australian Eragrostis species that are more closely related to target E. curvula than to the non-target E. plana and E. planiculmis. This suggests that Tetramesa sp. 4 may not be suitably host-specific for use as a biological control agent. Further host-specificity testing on these native Australian species is required, however, before this insect is ruled out completely. The Tetramesa on S. pyramidalis (Tetramesa sp. 1), and the unidentified Sporobolus species presumed to be S. africanus, were suitably host-specific to be used as biological control agents. Since it was unclear whether some phylogenetic clades were true species or intraspecific populations, which is essential to understand when selecting agents for biological control, a new piece of software, SPEDE-sampler", was developed. It offers users of the Generalised Mixed Yule Coalescent (GMYC) species delimitation model a means of assessing the degree to which sampling effects such as data size and parameter choice can influence species diversity estimates. When applied to the Tetramesa data set, the software assisted in identifying which groups may contain cryptic species, uncovering that the COI marker is affected more by singletons than the 28S marker (i.e. species diversity tends to be overestimated), and confirming putative Tetramesa taxa that could be useful for biological control programmes going forward. This thesis has provided evidence that South Africa contains a diverse assemblage of Tetramesa and other eurytomids that are closely associated with their grass hosts, and that many of these taxa hold promise for grass biological control. This work has also highlighted the importance of integrative taxonomy in the discovery of novel taxa, and that biological control practitioners need to be aware of the caveats of each line of evidence used in the delimitation of putative species. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Van Steenderen, Clarke Julian Mignon
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432565 , vital:72881 , DOI 10.21504/10962/432565
- Description: South Africa is a larger donor than receiver of alien grasses, where approximately 15% (_ 165 spp.) of the country's native grass species have become naturalised elsewhere. Many of these grasses have become serious invaders, causing significant damage to native species, habitat structure, and ecosystem functioning. Biological control is a sustainable and cost-effective method for the control of invasive weeds, but its application to invasive grasses has been approached with trepidation in the past due to the fears of a lack of host-specific herbivores that may cause non-target damage to agriculturally-important crops. The Tetramesa Walker genus (Hymenoptera: Eurytomidae) is one of three genera in the family that feed exclusively on grasses, and have a record of being host-specific to a particular species, or complex of closely-related congeners. There are over 200 described Tetramesa species, but this taxonomic effort has occurred almost exclusively in the Northern Hemisphere. Only about 2% of the described species are from Africa, with none from southern Africa despite the high diversity of grasses in the region. The low morphological variability between many Tetramesa groups has made identification difficult, where there may in fact be multiple undiscovered cryptic species. This thesis generated genetic sequence data (mitochondrial COI and nuclear 28S) that revealed at least eight native southern African Tetramesa taxa that are new to science, focusing particularly on the assemblages associated with Eragrostis curvula Nees (African lovegrass) and Sporobolus pyramidalis Beauv. and S. natalensis Steud. (giant rat's tail grass) which are alien invasive pests in Australia. Approximately 200 eurytomid wasps were collected and sequenced from 19 grass species across six South African provinces. Additionally, 27 grass species were sequenced using four gene regions (rps16-trnK, rps16, rpl32-trnL, and ITS), which were added to existing sequence data to build a dataset comprising over 700 sequences. Field host ranges and the use of host grass genetic proxies were important in making inferences about the host-specificity of eurytomid wasps of interest. Nine Tetramesa groups appeared to be host-specific to a single grass species, while six Tetramesa were associated with multiple species in a single grass genus. Since S. pyramidalis, S. natalensis, S. africanus, Hyparrhenia hirta, E. trichophora, and Andropogon gayanus are weeds elsewhere, there are at least six potential Tetramesa biological control agents that have been identified. A high diversity of Tetramesa on grasses within the Eragrostis genus was reported, with at least four taxa associated with E. curvula. It is currently uncertain whether these taxa represent different cryptic species or intraspecific populations that are the result of geographic sub-structuring. No-choice host-specificity testing using Tetramesa sp. 4 on E. curvula revealed that the wasp could complete its lifecycle on two non-target African grasses; namely E. plana and E. planiculmis. The wasp was also recorded on other Eragrostis species in the field (namely E. biflora and E. capensis). Using grass genetic sequences obtained in this study, it was found that there are four native Australian Eragrostis species that are more closely related to target E. curvula than to the non-target E. plana and E. planiculmis. This suggests that Tetramesa sp. 4 may not be suitably host-specific for use as a biological control agent. Further host-specificity testing on these native Australian species is required, however, before this insect is ruled out completely. The Tetramesa on S. pyramidalis (Tetramesa sp. 1), and the unidentified Sporobolus species presumed to be S. africanus, were suitably host-specific to be used as biological control agents. Since it was unclear whether some phylogenetic clades were true species or intraspecific populations, which is essential to understand when selecting agents for biological control, a new piece of software, SPEDE-sampler", was developed. It offers users of the Generalised Mixed Yule Coalescent (GMYC) species delimitation model a means of assessing the degree to which sampling effects such as data size and parameter choice can influence species diversity estimates. When applied to the Tetramesa data set, the software assisted in identifying which groups may contain cryptic species, uncovering that the COI marker is affected more by singletons than the 28S marker (i.e. species diversity tends to be overestimated), and confirming putative Tetramesa taxa that could be useful for biological control programmes going forward. This thesis has provided evidence that South Africa contains a diverse assemblage of Tetramesa and other eurytomids that are closely associated with their grass hosts, and that many of these taxa hold promise for grass biological control. This work has also highlighted the importance of integrative taxonomy in the discovery of novel taxa, and that biological control practitioners need to be aware of the caveats of each line of evidence used in the delimitation of putative species. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
Composition and physiological roles of gut microbiota in the False Coding Moth (Thaumatotibia leucotreta)
- Authors: Richardson, Perryn Heather
- Date: 2023-10-13
- Subjects: False codling moth , Microbiomes , Insect physiology , Citrus Diseases and pests South Africa , Biological pest control , Cryptophlebia leucotreta
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424457 , vital:72155
- Description: Gut microbiota can have a profound influence on host performance, behaviour and fitness. For False Codling Moth (FCM), Thaumatotibia leucotreta (Lepidoptera: Tortricidae), a major pest of citrus in South Africa, little work has been undertaken to date on gut microbe diversity or its influence on the host. This thesis aimed to i) characterise the gut microbiome of FCM under laboratory conditions and in FCM from the field, ii) and produce moths with reduced gut microbiota through egg dechorionation, which was followed by iii) the measurement of a suite of physiological traits, namely mass, survival and thermal stress in FCM from normal laboratory, dechorionated laboratory and field collected larvae that may be indicative of overall field performance. We aimed to directly test the hypothesis that gut microbial diversity partly determines insect performance and fitness by measuring its effects on growth, development, and tolerance to cold temperatures in FCM. FCM eggs that underwent dechorionation with sodium hypochlorite had an overall effect on larval survival, egg morphology and both larval and adult moth physiological measures. Increasing concentrations of sodium hypochlorite significantly decreased insect survival, (𝜒2(1, n = 10 850) = 21.724, p-value < 0.0001), with a concentration of ≈3.69% as the concentration limit (p-value < 0.001). Successful dechorionation of FCM was achieved with a wash of sodium hypochlorite at around 3.69% concentration and was visually confirmed by reduction of FCM egg surface area, (𝜒2(25, n = 260) p-value < 0.0001) and Scanning Electron Micrographs of the egg morphology. The gut microbiome of FCM from the different focus treatments was successfully characterized. Identification of the dominant bacterial families in these microbiomes revealed Xanthobacteraceae, Beijerinckiaceae and Burkholderiaceae in both the laboratory reared and field collected larvae, which suggests their systematic association with T. leucotreta. The most abundant genera were revealed as Bradyrhizobium, Methylobacterium and Burkholderia-Caballeronia-Paraburkholderia. Comparison of larval mass showed that treatment (dechorionated or not) had a significant effect on larval mass (𝜒2(2, n = 230) = 22.703, p-value < 0.001), field larvae were heavier than both control larvae and larvae with a disrupted gut microbiome. However, adult insects with a disrupted gut microbiome had more mass than individuals from the control and field-collected larvae with intact gut microbiomes (𝜒2(2, n = 230) = 39.074, p-value < 0.001). Despite the difference in mass between larval treatments, there was no significant difference in relative protein (𝜒2(2, n = 24) = 5.680, p-value = 0.06), carbohydrate (𝜒2(2, n = 24) = 3.940, p-value = 0.14) or lipid (𝜒2(2, n = 24) = 6.032, p-value = 0.05) content between individuals from the control and dechorionated treatments and field-collected individuals. Turning to thermal physiology, insects collected from the field took significantly longer to recover from chill coma than both laboratory treatments with intact and disrupted gut microbiomes (𝜒2(2, n = 129 = 39.659, p-value < 0.001). In addition, exposure to cold stress showed that treatment had a significant effect on insect mortality (𝜒2(2, n = 272) = 9.176, p-value = 0.01), with individuals from the control and dechorionated treatment being less likely to die after experiencing cold stress compared to field-collected individuals. Differences in the mass and thermal tolerance of insects with intact and disrupted gut microbiota suggest that gut microbiota may play an important role in the cold performance of T. leucotreta, and these findings constitute the basis for future molecular work on the functions of these bacterial taxa. This research highlights the need for consideration of the effects of T. leucotreta microbiome in current pest control programs. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Richardson, Perryn Heather
- Date: 2023-10-13
- Subjects: False codling moth , Microbiomes , Insect physiology , Citrus Diseases and pests South Africa , Biological pest control , Cryptophlebia leucotreta
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424457 , vital:72155
- Description: Gut microbiota can have a profound influence on host performance, behaviour and fitness. For False Codling Moth (FCM), Thaumatotibia leucotreta (Lepidoptera: Tortricidae), a major pest of citrus in South Africa, little work has been undertaken to date on gut microbe diversity or its influence on the host. This thesis aimed to i) characterise the gut microbiome of FCM under laboratory conditions and in FCM from the field, ii) and produce moths with reduced gut microbiota through egg dechorionation, which was followed by iii) the measurement of a suite of physiological traits, namely mass, survival and thermal stress in FCM from normal laboratory, dechorionated laboratory and field collected larvae that may be indicative of overall field performance. We aimed to directly test the hypothesis that gut microbial diversity partly determines insect performance and fitness by measuring its effects on growth, development, and tolerance to cold temperatures in FCM. FCM eggs that underwent dechorionation with sodium hypochlorite had an overall effect on larval survival, egg morphology and both larval and adult moth physiological measures. Increasing concentrations of sodium hypochlorite significantly decreased insect survival, (𝜒2(1, n = 10 850) = 21.724, p-value < 0.0001), with a concentration of ≈3.69% as the concentration limit (p-value < 0.001). Successful dechorionation of FCM was achieved with a wash of sodium hypochlorite at around 3.69% concentration and was visually confirmed by reduction of FCM egg surface area, (𝜒2(25, n = 260) p-value < 0.0001) and Scanning Electron Micrographs of the egg morphology. The gut microbiome of FCM from the different focus treatments was successfully characterized. Identification of the dominant bacterial families in these microbiomes revealed Xanthobacteraceae, Beijerinckiaceae and Burkholderiaceae in both the laboratory reared and field collected larvae, which suggests their systematic association with T. leucotreta. The most abundant genera were revealed as Bradyrhizobium, Methylobacterium and Burkholderia-Caballeronia-Paraburkholderia. Comparison of larval mass showed that treatment (dechorionated or not) had a significant effect on larval mass (𝜒2(2, n = 230) = 22.703, p-value < 0.001), field larvae were heavier than both control larvae and larvae with a disrupted gut microbiome. However, adult insects with a disrupted gut microbiome had more mass than individuals from the control and field-collected larvae with intact gut microbiomes (𝜒2(2, n = 230) = 39.074, p-value < 0.001). Despite the difference in mass between larval treatments, there was no significant difference in relative protein (𝜒2(2, n = 24) = 5.680, p-value = 0.06), carbohydrate (𝜒2(2, n = 24) = 3.940, p-value = 0.14) or lipid (𝜒2(2, n = 24) = 6.032, p-value = 0.05) content between individuals from the control and dechorionated treatments and field-collected individuals. Turning to thermal physiology, insects collected from the field took significantly longer to recover from chill coma than both laboratory treatments with intact and disrupted gut microbiomes (𝜒2(2, n = 129 = 39.659, p-value < 0.001). In addition, exposure to cold stress showed that treatment had a significant effect on insect mortality (𝜒2(2, n = 272) = 9.176, p-value = 0.01), with individuals from the control and dechorionated treatment being less likely to die after experiencing cold stress compared to field-collected individuals. Differences in the mass and thermal tolerance of insects with intact and disrupted gut microbiota suggest that gut microbiota may play an important role in the cold performance of T. leucotreta, and these findings constitute the basis for future molecular work on the functions of these bacterial taxa. This research highlights the need for consideration of the effects of T. leucotreta microbiome in current pest control programs. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
Distribution and habitat preferences of marine megafauna in Nosy Be, Madagascar
- Authors: Lubbé, Alicea
- Date: 2023-10-13
- Subjects: Marine animals Habitat Madagascar Nosy Be , Marine animals Geographical distribution Madagascar Nosy Be , Species distribution modelling , Maximum entropy method , Citizen science , Habitat preference
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424710 , vital:72177
- Description: Marine megafauna have important ecological roles including the top-down regulation of lower trophic levels and the transport of nutrients. They are also charismatic species of socioeconomic importance, due to their public appeal and focus for ecotourism. However, these taxa face numerous anthropogenic threats including bycatch, habitat-loss, noise disturbance, prey reductions, pollution, and vessel traffic. Northwest Madagascar has been identified as an important marine mammal area and whale shark aggregation site, yet limited information on megafauna within Madagascar exists. In this thesis, I investigated the community structure, distribution trends, related environmental factors (Chapter 2) and predicted habitat suitability (Chapter 3) using a species distribution modelling approach for marine megafauna around Nosy Be, North-west Madagascar. Data collection consisted of opportunistic boatbased surveys conducted while looking for whale sharks, mobulid rays and cetaceans. These activities were performed by dedicated ‘swim with whale shark’ tourism operators, Les Baleines Rand'eau and Safari Baleine during tourism activities directed by the Madagascar Whale Shark Project over four years (2016-2019). A total of 1792 sightings were reported with 13 identified species, including elasmobranchs: whale shark, Rhincodon typus; spinetail mobula ray, Mobula japonica; giant oceanic manta ray, Manta birostris; shortfin devil ray, Mobula kuhlii; balaenids: humpback whale, Megaptera novaeangliae; Omura's whale, Balaenoptera omurai, delphinids: Indo-Pacific bottlenose dolphin, Tursiops aduncus; spinner dolphin, Stenella longirostris; pantropical spotted dolphin, Stenella attenuata; Indian ocean humpback dolphin, Sousa plumbea; false killer whale, Pseudorca crassidens; melonheaded whale, Peponocephala electra and one cheloniid species: leatherback sea turtle, Dermochelys coriacea. Sightings were spatially analysed at a community level according to feeding guilds (i.e., filter feeders vs. predators), and distribution of the megafauna groups v were compared to selected remotely sensed physico-chemical data (sea surface temperature and chlorophyll-a concentration) and bathymetry charts (depth and slope) using generalized linear models (Chapter 2). All four variables had a significant effect on filter feeder distribution. Spatial distribution of filter feeder sightings peaked at an area of steep underwater topography (18-50m; x slope=0.5%) located on the west coast of Nosy Be, where foraging aggregations of large filter feeders (primarily R. typus and B. omurai) were frequently observed. Only SST had a significant effect on the relative abundance of animals in the predator feeding guild, which demonstrated a more longitudinal distribution along the continental shelf. The maximum entropy model (Maxent) was used for predicting habitat preference for the most frequently sighted species (Chapter 3). The distribution of whale sharks, mobulid rays and Omura’s whales were significantly overlapped as they were abundant in two main hotspots, Grand banc de l’entrée (~10 km of the west coast) and near Nosy Mitsio island, (~50 km northeast of Nosy Be). Suitable habitat for the dolphins was in relatively shallow waters in temperatures <28ºC near areas of steep changes in bathymetry. Bottlenose and spinner dolphins had a more widespread distribution across the continental shelf and humpback dolphin was closely associated with inshore reefs and occur in waters <10m. The coastal and inshore region of Nosy Be is an important habitat for a variety of megafaunal species, and the continental shelf provided essential areas for feeding and breeding. The results were similar to those presented for these species in other regions of the Indian Ocean (i.e., Eastern and Southern Africa; Western Australia; Seychelles) as well as outside of the Indian Ocean (i.e., Indonesia; Caribbean; North Atlantic). Thus the results from this thesis could be considered for management recommendations for marine conservation in the region. The value and limitations of data collected by citizen science were outlined (Chapter 4). , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Lubbé, Alicea
- Date: 2023-10-13
- Subjects: Marine animals Habitat Madagascar Nosy Be , Marine animals Geographical distribution Madagascar Nosy Be , Species distribution modelling , Maximum entropy method , Citizen science , Habitat preference
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424710 , vital:72177
- Description: Marine megafauna have important ecological roles including the top-down regulation of lower trophic levels and the transport of nutrients. They are also charismatic species of socioeconomic importance, due to their public appeal and focus for ecotourism. However, these taxa face numerous anthropogenic threats including bycatch, habitat-loss, noise disturbance, prey reductions, pollution, and vessel traffic. Northwest Madagascar has been identified as an important marine mammal area and whale shark aggregation site, yet limited information on megafauna within Madagascar exists. In this thesis, I investigated the community structure, distribution trends, related environmental factors (Chapter 2) and predicted habitat suitability (Chapter 3) using a species distribution modelling approach for marine megafauna around Nosy Be, North-west Madagascar. Data collection consisted of opportunistic boatbased surveys conducted while looking for whale sharks, mobulid rays and cetaceans. These activities were performed by dedicated ‘swim with whale shark’ tourism operators, Les Baleines Rand'eau and Safari Baleine during tourism activities directed by the Madagascar Whale Shark Project over four years (2016-2019). A total of 1792 sightings were reported with 13 identified species, including elasmobranchs: whale shark, Rhincodon typus; spinetail mobula ray, Mobula japonica; giant oceanic manta ray, Manta birostris; shortfin devil ray, Mobula kuhlii; balaenids: humpback whale, Megaptera novaeangliae; Omura's whale, Balaenoptera omurai, delphinids: Indo-Pacific bottlenose dolphin, Tursiops aduncus; spinner dolphin, Stenella longirostris; pantropical spotted dolphin, Stenella attenuata; Indian ocean humpback dolphin, Sousa plumbea; false killer whale, Pseudorca crassidens; melonheaded whale, Peponocephala electra and one cheloniid species: leatherback sea turtle, Dermochelys coriacea. Sightings were spatially analysed at a community level according to feeding guilds (i.e., filter feeders vs. predators), and distribution of the megafauna groups v were compared to selected remotely sensed physico-chemical data (sea surface temperature and chlorophyll-a concentration) and bathymetry charts (depth and slope) using generalized linear models (Chapter 2). All four variables had a significant effect on filter feeder distribution. Spatial distribution of filter feeder sightings peaked at an area of steep underwater topography (18-50m; x slope=0.5%) located on the west coast of Nosy Be, where foraging aggregations of large filter feeders (primarily R. typus and B. omurai) were frequently observed. Only SST had a significant effect on the relative abundance of animals in the predator feeding guild, which demonstrated a more longitudinal distribution along the continental shelf. The maximum entropy model (Maxent) was used for predicting habitat preference for the most frequently sighted species (Chapter 3). The distribution of whale sharks, mobulid rays and Omura’s whales were significantly overlapped as they were abundant in two main hotspots, Grand banc de l’entrée (~10 km of the west coast) and near Nosy Mitsio island, (~50 km northeast of Nosy Be). Suitable habitat for the dolphins was in relatively shallow waters in temperatures <28ºC near areas of steep changes in bathymetry. Bottlenose and spinner dolphins had a more widespread distribution across the continental shelf and humpback dolphin was closely associated with inshore reefs and occur in waters <10m. The coastal and inshore region of Nosy Be is an important habitat for a variety of megafaunal species, and the continental shelf provided essential areas for feeding and breeding. The results were similar to those presented for these species in other regions of the Indian Ocean (i.e., Eastern and Southern Africa; Western Australia; Seychelles) as well as outside of the Indian Ocean (i.e., Indonesia; Caribbean; North Atlantic). Thus the results from this thesis could be considered for management recommendations for marine conservation in the region. The value and limitations of data collected by citizen science were outlined (Chapter 4). , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
Enteric microbial responses to host hyperthermia in southern African insectivorous bats: a test of thermal co-adaptation
- Authors: Hunter, Amy Alison
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424391 , vital:72149
- Description: Embargoed. Expected release date 2025. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Hunter, Amy Alison
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424391 , vital:72149
- Description: Embargoed. Expected release date 2025. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
Evaluation of potential oviposition deterrents for false codling moth, Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae)
- Authors: Dambuza, Khalipha
- Date: 2023-10-13
- Subjects: Cryptophlebia leucotreta , Pests Integrated control , Semiochemicals , Agricultural pests Control , Oviposition , Essences and essential oils
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424479 , vital:72157
- Description: There has been extensive research on the use of semiochemicals as deterrents or true repellents in insect pest management, particularly in push-pull strategies. Much of this research has focused on pests of medical and veterinary importance and has been limited for agricultural pests. This means there is an opportunity to study use of deterrents to manage pests of agricultural importance. No study has been conducted on deterrents for false codling moth (FCM), Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae), a key phytosanitary pest in citrus orchards across South Africa. This study assessed FCM oviposition deterrence in botanicals (plants (n = 11) and essential oils (n = 15)), and some commercial pesticides (n = 7) used for FCM control in South Africa. All tested botanicals were selected based on an extensive literature review of plant compounds that have been reported to deter or repel lepidopteran pests. Choice and no-choice oviposition bioassays were conducted in complete darkness in a controlled environment room. Oranges treated with solutions/suspensions of potential oviposition deterrents were placed into a cage with gravid FCM females for four hours, with oviposition being recorded every hour. Of the 33 tested compounds, only eight significantly reduced FCM oviposition (P < 0.05) compared to the control in oviposition bioassays i.e. two essential oils (lavender and peppermint), two plant crude extracts (garlic and marigold), one fruit (Mango), and three commercial FCM insecticides (Delegate, Coragen, and Warlock). All identified oviposition deterrents, except for Mango, were further investigated for their ovicidal properties in concentration response bioassays, where all botanicals were identified to have dual action (both deterrent and ovicidal properties), as they significantly (P < 0.05) reduced FCM oviposition and egg hatch. Garlic was the most efficacious botanical whilst Warlock was the only commercial insecticide that did not show ovicidal activity (F = 41.17, P = 0.0622). Larval penetration of the host fruit was less than egg hatch for all tested compounds in concentration response bioassays. Oviposition, egg hatch, and larval penetration were all affected by concentration, with the higher concentrations being the most effective. The efficacy of these deterrent compounds should be further tested in semi-field and/or field trials, and they may have potential in FCM management as allomone dispensers or sprays. They can also be implemented in push-pull strategies where they can be used in conjunction with FCM attractants. Lastly, repellence studies should be conducted in absentia of the host fruit to determine whether oviposition deterrence was a result of true repellence or odour masking. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Dambuza, Khalipha
- Date: 2023-10-13
- Subjects: Cryptophlebia leucotreta , Pests Integrated control , Semiochemicals , Agricultural pests Control , Oviposition , Essences and essential oils
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424479 , vital:72157
- Description: There has been extensive research on the use of semiochemicals as deterrents or true repellents in insect pest management, particularly in push-pull strategies. Much of this research has focused on pests of medical and veterinary importance and has been limited for agricultural pests. This means there is an opportunity to study use of deterrents to manage pests of agricultural importance. No study has been conducted on deterrents for false codling moth (FCM), Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae), a key phytosanitary pest in citrus orchards across South Africa. This study assessed FCM oviposition deterrence in botanicals (plants (n = 11) and essential oils (n = 15)), and some commercial pesticides (n = 7) used for FCM control in South Africa. All tested botanicals were selected based on an extensive literature review of plant compounds that have been reported to deter or repel lepidopteran pests. Choice and no-choice oviposition bioassays were conducted in complete darkness in a controlled environment room. Oranges treated with solutions/suspensions of potential oviposition deterrents were placed into a cage with gravid FCM females for four hours, with oviposition being recorded every hour. Of the 33 tested compounds, only eight significantly reduced FCM oviposition (P < 0.05) compared to the control in oviposition bioassays i.e. two essential oils (lavender and peppermint), two plant crude extracts (garlic and marigold), one fruit (Mango), and three commercial FCM insecticides (Delegate, Coragen, and Warlock). All identified oviposition deterrents, except for Mango, were further investigated for their ovicidal properties in concentration response bioassays, where all botanicals were identified to have dual action (both deterrent and ovicidal properties), as they significantly (P < 0.05) reduced FCM oviposition and egg hatch. Garlic was the most efficacious botanical whilst Warlock was the only commercial insecticide that did not show ovicidal activity (F = 41.17, P = 0.0622). Larval penetration of the host fruit was less than egg hatch for all tested compounds in concentration response bioassays. Oviposition, egg hatch, and larval penetration were all affected by concentration, with the higher concentrations being the most effective. The efficacy of these deterrent compounds should be further tested in semi-field and/or field trials, and they may have potential in FCM management as allomone dispensers or sprays. They can also be implemented in push-pull strategies where they can be used in conjunction with FCM attractants. Lastly, repellence studies should be conducted in absentia of the host fruit to determine whether oviposition deterrence was a result of true repellence or odour masking. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
Freshwater invasive alien crayfish species in South Africa: testing eDNA assessment and DNA barcoding
- Mdidimba, Nonkazimulo Dorothea
- Authors: Mdidimba, Nonkazimulo Dorothea
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424413 , vital:72151
- Description: The expansion of the wild populations of the two invasive alien crayfish species, i.e., Cherax quadricarinatus and Procambarus clarkii in South Africa is of high concern. Given this, innovative and improved monitoring methods are required to be explored in order to effectively detect and manage their spread. Environmental DNA (eDNA) has shown promising and reliable results to detect and monitor alien crayfish in freshwater systems even at low densities. This research study aimed to field-test the application of eDNA for detecting and monitoring invasive alien freshwater crayfish species (IAFCs) in South African freshwater systems (Chapter 2). Further, this study also aimed to confirm the true taxonomic identity of the IAFCs in South African freshwater systems by using DNA barcoding to avoid potential misidentifications with morphological identification alone (Chapter 3). Also, ectocommensal temnocephalans and leeches attached to the crayfishes were also identified with DNA barcoding. The mitochondrial COI gene fragment of the crayfishes and the ectocommensal temnocephalans and leeches was amplified, sequenced, and used for species identification. For eDNA monitoring of C. cainii, C. quadricarinatus and P. clarkii, water samples were collected from Eastern Cape, Free State and KwaZulu Natal sites that are known to house these species. The current study did not detect the eDNA of the wild populations of both C. quadricarinatus and P. clarkii. This failure was encountered despite employing several methods, such as, end-point polymerase chain reaction (PCR), quantitative PCR (qPCR) and next-generation sequencing (NGS). The eDNA assessment to detect IAFCs in the wild populations is discussed in detail, and corrective measures suggested (Chapter 2). However, only C. cainii was able to be detected by eDNA. Cherax cainii is housed/farmed in an aquaculture facility (Vaughn Bursey’s Stock Farm in Eastern Cape) with large number of individuals per pond. For this, NGS was able to detect C. cainii but the other two methods, end-point PCR and qPCR were not able to detect the species. Based on the results, refinement of eDNA monitoring methods for the South African freshwater systems is recommended. Evaluation of different DNA collection methods, preservation, DNA extraction kits and primer design to obtain effective eDNA detection is discussed as possible sources of error. Interestingly, the newly established population of P. clarkii in Mimosa Dam in Free State was infested with alien leeches, that were confirmed by DNA barcoding to be Helobdella europaea and Helobdella octatestica. Consequently, this study reports for the first time in South Africa the presence of this alien freshwater leeches. The presence of alien leeches and P. clarkii suggests a double invasion in Mimosa Dam, P. clarkii seems to continue expanding its range in the country. However, the long-established population of P. clarkii in Driehoek Farm in Mpumalanga, which has been in the country for over 30 years did not have any leech or temnocephalan infestation. This could suggest several interesting ecological phenomena, including environmental filtering over time of the co-invaders, differential source populations, and post-introduction adaptation. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Mdidimba, Nonkazimulo Dorothea
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424413 , vital:72151
- Description: The expansion of the wild populations of the two invasive alien crayfish species, i.e., Cherax quadricarinatus and Procambarus clarkii in South Africa is of high concern. Given this, innovative and improved monitoring methods are required to be explored in order to effectively detect and manage their spread. Environmental DNA (eDNA) has shown promising and reliable results to detect and monitor alien crayfish in freshwater systems even at low densities. This research study aimed to field-test the application of eDNA for detecting and monitoring invasive alien freshwater crayfish species (IAFCs) in South African freshwater systems (Chapter 2). Further, this study also aimed to confirm the true taxonomic identity of the IAFCs in South African freshwater systems by using DNA barcoding to avoid potential misidentifications with morphological identification alone (Chapter 3). Also, ectocommensal temnocephalans and leeches attached to the crayfishes were also identified with DNA barcoding. The mitochondrial COI gene fragment of the crayfishes and the ectocommensal temnocephalans and leeches was amplified, sequenced, and used for species identification. For eDNA monitoring of C. cainii, C. quadricarinatus and P. clarkii, water samples were collected from Eastern Cape, Free State and KwaZulu Natal sites that are known to house these species. The current study did not detect the eDNA of the wild populations of both C. quadricarinatus and P. clarkii. This failure was encountered despite employing several methods, such as, end-point polymerase chain reaction (PCR), quantitative PCR (qPCR) and next-generation sequencing (NGS). The eDNA assessment to detect IAFCs in the wild populations is discussed in detail, and corrective measures suggested (Chapter 2). However, only C. cainii was able to be detected by eDNA. Cherax cainii is housed/farmed in an aquaculture facility (Vaughn Bursey’s Stock Farm in Eastern Cape) with large number of individuals per pond. For this, NGS was able to detect C. cainii but the other two methods, end-point PCR and qPCR were not able to detect the species. Based on the results, refinement of eDNA monitoring methods for the South African freshwater systems is recommended. Evaluation of different DNA collection methods, preservation, DNA extraction kits and primer design to obtain effective eDNA detection is discussed as possible sources of error. Interestingly, the newly established population of P. clarkii in Mimosa Dam in Free State was infested with alien leeches, that were confirmed by DNA barcoding to be Helobdella europaea and Helobdella octatestica. Consequently, this study reports for the first time in South Africa the presence of this alien freshwater leeches. The presence of alien leeches and P. clarkii suggests a double invasion in Mimosa Dam, P. clarkii seems to continue expanding its range in the country. However, the long-established population of P. clarkii in Driehoek Farm in Mpumalanga, which has been in the country for over 30 years did not have any leech or temnocephalan infestation. This could suggest several interesting ecological phenomena, including environmental filtering over time of the co-invaders, differential source populations, and post-introduction adaptation. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
Native-range studies on insect herbivores associated with African Lovegrass (Eragrostis curvula) in South Africa: prospects for biological control in Australia
- Authors: Yell, Liam Dougal
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424446 , vital:72154
- Description: Eragrostis curvula (Schrad.) Nees. (African Lovegrass) is an African native species of grass that was intentionally introduced for pasture in Australia. It has since escaped cultivation and has become widespread and highly invasive. Eragrostis curvula has been recorded in every state and territory in Australia where it has altered fire regimes, disrupted nutrient cycles and can reduce livestock carrying capacity by up to 50%. The Centre for Biological Control at Rhodes University and the New South Wales Department of Primary Industries have been working in collaboration to identify and screen herbivorous insects as biological control agents for E. curvula in Australia. Native-range surveys were conducted between 2021 and 2022 on E. curvula at twenty-two sites across South Africa to identify herbivorous natural enemies associated with it. Species accumulation curves were generated to ensure adequate sampling was performed to identify all the insects associated with E. curvula. Twenty-nine non-target grass species were surveyed simultaneously to determine the field-host range of the natural enemies associated with the target weed. Herbivorous natural enemies were prioritised as possible biological control agents against E. curvula in Australia based on field-host range, predicted efficacy and climatic suitability. Four insect species were consistently found on E. curvula, two of which were herbivorous, as well as a parasitoid and a detritivore. Species accumulation curves show that the insect community was adequately sampled in South Africa. The two herbivorous insects were identified to the lowest taxonomic level using COI barcoding. Both species are undescribed phytophagous wasps in the genus Tetramesa (Hymenoptera: Eurytomidae). Because Tetramesa species have been shown to be host specific and highly damaging in previous biological control programs for other invasive grass weeds, we assessed their suitability as candidate biological control agents for use on E. curvula in Australia. Both Tetramesa species (“sp. 4” and “sp. 5”) were found on several native congeners under field conditions in South Africa. Congeneric South African-native non-target grass species were used as phylogenetic proxies to assess the risk posed to Australian native Eragrostis species. This highlighted three non-target Australian native Eragrostis species, namely: E. parviflora (R. Br.) Trin., E. leptocarpa Benth. fl., and E. trachycarpa Benth., that are at risk of being attacked by the two candidate agents based on their phylogenetic proximity to E. curvula. Predicted efficacy trials were conducted at five long-term repeat survey sites and revealed that Tetramesa sp. 4 does not reduce the probability of E. curvula tiller survival or reproduction, while Tetramesa sp. 5 does not reduce the probability of tiller reproduction but does increase the probability of tiller survival. This result was unexpected and may be a plant compensatory response to herbivory. The sites where both Tetramesa species were collected in South Africa are climatically similar to the invaded range of E. curvula in Australia, and as such, the Tetramesa spp. are likely to be suitably adapted to the climate where they would be released in Australia. These results suggest that both Tetramesa species associated with E. curvula may have too broad a host range to be used as biological control agents in Australia. However, further quarantine-based host-range assessments on Australian native Eragrostis species are recommended to confirm this. The field-based methods used in this study have reduced the number of insect and plant species that host-range assessments will be required to be performed on, thus preventing wasted resources. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Yell, Liam Dougal
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424446 , vital:72154
- Description: Eragrostis curvula (Schrad.) Nees. (African Lovegrass) is an African native species of grass that was intentionally introduced for pasture in Australia. It has since escaped cultivation and has become widespread and highly invasive. Eragrostis curvula has been recorded in every state and territory in Australia where it has altered fire regimes, disrupted nutrient cycles and can reduce livestock carrying capacity by up to 50%. The Centre for Biological Control at Rhodes University and the New South Wales Department of Primary Industries have been working in collaboration to identify and screen herbivorous insects as biological control agents for E. curvula in Australia. Native-range surveys were conducted between 2021 and 2022 on E. curvula at twenty-two sites across South Africa to identify herbivorous natural enemies associated with it. Species accumulation curves were generated to ensure adequate sampling was performed to identify all the insects associated with E. curvula. Twenty-nine non-target grass species were surveyed simultaneously to determine the field-host range of the natural enemies associated with the target weed. Herbivorous natural enemies were prioritised as possible biological control agents against E. curvula in Australia based on field-host range, predicted efficacy and climatic suitability. Four insect species were consistently found on E. curvula, two of which were herbivorous, as well as a parasitoid and a detritivore. Species accumulation curves show that the insect community was adequately sampled in South Africa. The two herbivorous insects were identified to the lowest taxonomic level using COI barcoding. Both species are undescribed phytophagous wasps in the genus Tetramesa (Hymenoptera: Eurytomidae). Because Tetramesa species have been shown to be host specific and highly damaging in previous biological control programs for other invasive grass weeds, we assessed their suitability as candidate biological control agents for use on E. curvula in Australia. Both Tetramesa species (“sp. 4” and “sp. 5”) were found on several native congeners under field conditions in South Africa. Congeneric South African-native non-target grass species were used as phylogenetic proxies to assess the risk posed to Australian native Eragrostis species. This highlighted three non-target Australian native Eragrostis species, namely: E. parviflora (R. Br.) Trin., E. leptocarpa Benth. fl., and E. trachycarpa Benth., that are at risk of being attacked by the two candidate agents based on their phylogenetic proximity to E. curvula. Predicted efficacy trials were conducted at five long-term repeat survey sites and revealed that Tetramesa sp. 4 does not reduce the probability of E. curvula tiller survival or reproduction, while Tetramesa sp. 5 does not reduce the probability of tiller reproduction but does increase the probability of tiller survival. This result was unexpected and may be a plant compensatory response to herbivory. The sites where both Tetramesa species were collected in South Africa are climatically similar to the invaded range of E. curvula in Australia, and as such, the Tetramesa spp. are likely to be suitably adapted to the climate where they would be released in Australia. These results suggest that both Tetramesa species associated with E. curvula may have too broad a host range to be used as biological control agents in Australia. However, further quarantine-based host-range assessments on Australian native Eragrostis species are recommended to confirm this. The field-based methods used in this study have reduced the number of insect and plant species that host-range assessments will be required to be performed on, thus preventing wasted resources. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
Phylogeography of two small owl species in South Africa: population genetics and phenotypic variation in the African Barred Owlet (Glaucidium capense) and the African Scops Owl (Otus senegalensis)
- Authors: Balmer, Jonathan Peter
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424369 , vital:72147
- Description: Embargoed. Expected release date in 2025. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Balmer, Jonathan Peter
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424369 , vital:72147
- Description: Embargoed. Expected release date in 2025. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-10-13