The Emancipatory Nature of Transformative Agency
- Lotz-Sisitka, Heila, Thifhulufhelwi, Reuben, Chikunda, Charles, Mponwana, Maletje
- Authors: Lotz-Sisitka, Heila , Thifhulufhelwi, Reuben , Chikunda, Charles , Mponwana, Maletje
- Date: 2023
- Subjects: To be catalogued
- Language: English
- Type: text , book chapter
- Identifier: http://hdl.handle.net/10962/436175 , vital:73232 , ISBN 9781009153799 , https://doi.org/10.1017/9781009153799
- Description: Cultural-historical activity theory (CHAT) research has established an impressive body of scholarship that advances social understanding of human agency in the transformation of society, much of which is captured in contributions to this volume (cf. Hopwood (Chapter 15); Sannino (Chapter 2); Stetsenko (Chapter 3); Bal and Bird Bear (Chapter 8)). The main tenets of this work, building on the historical legacy of Vygotsky and Marx, affirm that humans are not passive recipients of external stimuli or influences but are active co-creators of the world (s) they inhabit. They are capable of using and producing cultural tools to take power over their own volitional action (s)(cf. Sannino, 2015, Chap-ter 2 of this volume; Hopwood and Gottshalk, 2017). Vygotsky’s major legacy in coming to understand transformative agency is that the cultural tools produced and used as mediational means are critical in the emergence of transformative agency (Sannino, 2015, 2020, Chapter 2 of this volume; Stetsenko, 2019; Hopwood, Chapter 15 of this volume) and thus in the transformation of human activity. As shown by Sannino (2015, 2020), such tools offer stimulus for volitional action that can break paralysis, especially when conflicts of motives are experienced.
- Full Text:
- Date Issued: 2023
- Authors: Lotz-Sisitka, Heila , Thifhulufhelwi, Reuben , Chikunda, Charles , Mponwana, Maletje
- Date: 2023
- Subjects: To be catalogued
- Language: English
- Type: text , book chapter
- Identifier: http://hdl.handle.net/10962/436175 , vital:73232 , ISBN 9781009153799 , https://doi.org/10.1017/9781009153799
- Description: Cultural-historical activity theory (CHAT) research has established an impressive body of scholarship that advances social understanding of human agency in the transformation of society, much of which is captured in contributions to this volume (cf. Hopwood (Chapter 15); Sannino (Chapter 2); Stetsenko (Chapter 3); Bal and Bird Bear (Chapter 8)). The main tenets of this work, building on the historical legacy of Vygotsky and Marx, affirm that humans are not passive recipients of external stimuli or influences but are active co-creators of the world (s) they inhabit. They are capable of using and producing cultural tools to take power over their own volitional action (s)(cf. Sannino, 2015, Chap-ter 2 of this volume; Hopwood and Gottshalk, 2017). Vygotsky’s major legacy in coming to understand transformative agency is that the cultural tools produced and used as mediational means are critical in the emergence of transformative agency (Sannino, 2015, 2020, Chapter 2 of this volume; Stetsenko, 2019; Hopwood, Chapter 15 of this volume) and thus in the transformation of human activity. As shown by Sannino (2015, 2020), such tools offer stimulus for volitional action that can break paralysis, especially when conflicts of motives are experienced.
- Full Text:
- Date Issued: 2023
Evaluation of Intussusception After Oral Monovalent Rotavirus Vaccination in South Africa
- Michelle J. Groome, Nick Andrews, Jacqueline E. Tate, Marion Arnold, Milind Chitnis, Sharon Cox, Corné de Vos, Mari Kirsten, Susanna M. le Grange, Jerome Loveland, Sello Machaea, Ashwini Maharaj, Shabir A. Madhi, Umesh D. Parashar
- Authors: Michelle J. Groome , Nick Andrews , Jacqueline E. Tate , Marion Arnold , Milind Chitnis , Sharon Cox , Corné de Vos , Mari Kirsten , Susanna M. le Grange , Jerome Loveland , Sello Machaea , Ashwini Maharaj , Shabir A. Madhi , Umesh D. Parashar
- Date: 2021
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4543 , vital:44131
- Full Text:
- Authors: Michelle J. Groome , Nick Andrews , Jacqueline E. Tate , Marion Arnold , Milind Chitnis , Sharon Cox , Corné de Vos , Mari Kirsten , Susanna M. le Grange , Jerome Loveland , Sello Machaea , Ashwini Maharaj , Shabir A. Madhi , Umesh D. Parashar
- Date: 2021
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4543 , vital:44131
- Full Text:
Biomes, geology and past climate drive speciation of laminate-toothed rats on South African mountains (Murinae: Otomys)
- Taylor, Peter J, Kearney, Theresa, Dalton, Desire L, Chakona, Gamuchirai, Kelly, Christopher M R, Barker, Nigel P
- Authors: Taylor, Peter J , Kearney, Theresa , Dalton, Desire L , Chakona, Gamuchirai , Kelly, Christopher M R , Barker, Nigel P
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/453158 , vital:75227 , xlink:href="https://doi.org/10.1093/zoolinnean/zlz134"
- Description: Mitochondrial DNA sequences (1137 bp) of the cytochrome b gene and craniodental and craniometric data were used to investigate the evolutionary relationships of six putative rodent taxa of Otomys (family Muridae: subfamily Murinae: tribe Otomyini) co-occurring in the Western Cape and Eastern Cape provinces of South Africa. Phylogenetic analysis of 20 new sequences together with craniodental and craniometric characters of 94 adult skulls reveal the existence of a unique lineage of Otomys cf. karoensis (named herein Otomys willani sp. nov.) from the Sneeuberg Centre of Floristic Endemism in the southern Drakensberg Mountain Range. Craniometric analysis distinguished O. karoensis from O. willani and identified a further four localities in the range of the latter species. We document southern range extensions of both Sloggett’s ice rat, Otomys sloggetti, and the vlei rat Otomys auratus to the Sneeuberg Mountain Range, in addition to appreciable genetic divergence between Sneeuberg and southern and central Drakensberg populations of O. sloggetti. Our results demonstrate parallel patterns of cryptic speciation in two co-occurring species complexes (Otomys irroratus s.l. and O. karoensis s.l.) associated closely with the boundaries of biomes (fynbos vs. grassland biomes) and geological formations (Cape Fold Belt vs. Great Escarpment).
- Full Text:
- Date Issued: 2020
- Authors: Taylor, Peter J , Kearney, Theresa , Dalton, Desire L , Chakona, Gamuchirai , Kelly, Christopher M R , Barker, Nigel P
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/453158 , vital:75227 , xlink:href="https://doi.org/10.1093/zoolinnean/zlz134"
- Description: Mitochondrial DNA sequences (1137 bp) of the cytochrome b gene and craniodental and craniometric data were used to investigate the evolutionary relationships of six putative rodent taxa of Otomys (family Muridae: subfamily Murinae: tribe Otomyini) co-occurring in the Western Cape and Eastern Cape provinces of South Africa. Phylogenetic analysis of 20 new sequences together with craniodental and craniometric characters of 94 adult skulls reveal the existence of a unique lineage of Otomys cf. karoensis (named herein Otomys willani sp. nov.) from the Sneeuberg Centre of Floristic Endemism in the southern Drakensberg Mountain Range. Craniometric analysis distinguished O. karoensis from O. willani and identified a further four localities in the range of the latter species. We document southern range extensions of both Sloggett’s ice rat, Otomys sloggetti, and the vlei rat Otomys auratus to the Sneeuberg Mountain Range, in addition to appreciable genetic divergence between Sneeuberg and southern and central Drakensberg populations of O. sloggetti. Our results demonstrate parallel patterns of cryptic speciation in two co-occurring species complexes (Otomys irroratus s.l. and O. karoensis s.l.) associated closely with the boundaries of biomes (fynbos vs. grassland biomes) and geological formations (Cape Fold Belt vs. Great Escarpment).
- Full Text:
- Date Issued: 2020
Biomes, geology and past climate drive speciation of laminate-toothed rats on South African mountains (Murinae: Otomys)
- Taylor, Peter J, Kearney, Theresa, Dalton, Desire L, Chakona, Gamuchirai, Kelly, Christopher M R, Barker, Nigel P
- Authors: Taylor, Peter J , Kearney, Theresa , Dalton, Desire L , Chakona, Gamuchirai , Kelly, Christopher M R , Barker, Nigel P
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/425481 , vital:72249 , xlink:href="https://doi.org/10.1093/zoolinnean/zlz134"
- Description: Mitochondrial DNA sequences (1137 bp) of the cytochrome b gene and craniodental and craniometric data were used to investigate the evolutionary relationships of six putative rodent taxa of Otomys (family Muridae: subfamily Murinae: tribe Otomyini) co-occurring in the Western Cape and Eastern Cape provinces of South Africa. Phylogenetic analysis of 20 new sequences together with craniodental and craniometric characters of 94 adult skulls reveal the existence of a unique lineage of Otomys cf. karoensis (named herein Otomys willani sp. nov.) from the Sneeuberg Centre of Floristic Endemism in the southern Drakensberg Mountain Range. Craniometric analysis distinguished O. karoensis from O. willani and identified a further four localities in the range of the latter species. We document southern range extensions of both Sloggett’s ice rat, Otomys sloggetti, and the vlei rat Otomys auratus to the Sneeuberg Mountain Range, in addition to appreciable genetic divergence between Sneeuberg and southern and central Drakensberg populations of O. sloggetti. Our results demonstrate parallel patterns of cryptic speciation in two co-occurring species complexes (Otomys irroratus s.l. and O. karoensis s.l.) associated closely with the boundaries of biomes (fynbos vs. grassland biomes) and geological formations (Cape Fold Belt vs. Great Escarpment).
- Full Text:
- Date Issued: 2020
- Authors: Taylor, Peter J , Kearney, Theresa , Dalton, Desire L , Chakona, Gamuchirai , Kelly, Christopher M R , Barker, Nigel P
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/425481 , vital:72249 , xlink:href="https://doi.org/10.1093/zoolinnean/zlz134"
- Description: Mitochondrial DNA sequences (1137 bp) of the cytochrome b gene and craniodental and craniometric data were used to investigate the evolutionary relationships of six putative rodent taxa of Otomys (family Muridae: subfamily Murinae: tribe Otomyini) co-occurring in the Western Cape and Eastern Cape provinces of South Africa. Phylogenetic analysis of 20 new sequences together with craniodental and craniometric characters of 94 adult skulls reveal the existence of a unique lineage of Otomys cf. karoensis (named herein Otomys willani sp. nov.) from the Sneeuberg Centre of Floristic Endemism in the southern Drakensberg Mountain Range. Craniometric analysis distinguished O. karoensis from O. willani and identified a further four localities in the range of the latter species. We document southern range extensions of both Sloggett’s ice rat, Otomys sloggetti, and the vlei rat Otomys auratus to the Sneeuberg Mountain Range, in addition to appreciable genetic divergence between Sneeuberg and southern and central Drakensberg populations of O. sloggetti. Our results demonstrate parallel patterns of cryptic speciation in two co-occurring species complexes (Otomys irroratus s.l. and O. karoensis s.l.) associated closely with the boundaries of biomes (fynbos vs. grassland biomes) and geological formations (Cape Fold Belt vs. Great Escarpment).
- Full Text:
- Date Issued: 2020
Cultural adaptations of Dance Movement Psychotherapy experiences: from a UK higher education context to working with communities in southern Africa facing water related inequality
- Copteros, Athina, Karkou, Vicky, Palmer, Carolyn G
- Authors: Copteros, Athina , Karkou, Vicky , Palmer, Carolyn G
- Date: 2017
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/141995 , vital:38022 , ISBN 9780199949298 , https://doi.org/10.1093/oxfordhb/9780199949298.001.0001
- Description: Water plays a key role in all our lives and in South Africa epitomizes a space in which political inequalities have played themselves out with devastating consequences. The current ecological crisis demands new ways of engaging with ourselves, each other and nature. This research is an initial exploration on the use of a body-based creative movement approach within a transdisciplinary complex social-ecological systems researcher group. The research objective discussed in this chapter is to develop culturally relevant themes from professional Dance Movement Psychotherapy (DMP) training in the UK for application in a South African water resource management context. Interpretative Phenomenological Analysis was used to identify culturally relevant themes based on the recorded perceptions of the phenomenon of the training while it was taking place. The themes of: Awareness of Power and Difference; Therapeutic Adaptability; Sharing Leadership and Connecting with the Environment were identified. Artistic Inquiry was used to creatively reflect on the themes and add an embodied response to the discussion. The cultural adaptations of DMP can contribute to a more engaged and non-hierarchical collaboration between practitioners and the people and communities they serve, which would facilitate a therapeutic practice that works with both internal, external (and even transcendental) factors.
- Full Text:
- Date Issued: 2017
- Authors: Copteros, Athina , Karkou, Vicky , Palmer, Carolyn G
- Date: 2017
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/141995 , vital:38022 , ISBN 9780199949298 , https://doi.org/10.1093/oxfordhb/9780199949298.001.0001
- Description: Water plays a key role in all our lives and in South Africa epitomizes a space in which political inequalities have played themselves out with devastating consequences. The current ecological crisis demands new ways of engaging with ourselves, each other and nature. This research is an initial exploration on the use of a body-based creative movement approach within a transdisciplinary complex social-ecological systems researcher group. The research objective discussed in this chapter is to develop culturally relevant themes from professional Dance Movement Psychotherapy (DMP) training in the UK for application in a South African water resource management context. Interpretative Phenomenological Analysis was used to identify culturally relevant themes based on the recorded perceptions of the phenomenon of the training while it was taking place. The themes of: Awareness of Power and Difference; Therapeutic Adaptability; Sharing Leadership and Connecting with the Environment were identified. Artistic Inquiry was used to creatively reflect on the themes and add an embodied response to the discussion. The cultural adaptations of DMP can contribute to a more engaged and non-hierarchical collaboration between practitioners and the people and communities they serve, which would facilitate a therapeutic practice that works with both internal, external (and even transcendental) factors.
- Full Text:
- Date Issued: 2017
Public reproductive health and ‘unintended’ pregnancies: introducing the construct ‘supportability’
- Authors: Macleod, Catriona I
- Date: 2015
- Language: English
- Type: Article
- Identifier: vital:6313 , http://hdl.handle.net/10962/d1019881 , https://academic.oup.com/jpubhealth/article-lookup/doi/10.1093/pubmed/fdv123
- Description: In this Perspectives paper, I outline the limitations of the concept of ‘intentionality’ in public reproductive health understandings of pregnancy. ‘Intentionality’, ‘plannedness’, ‘wantedness’ and ‘timing’ place individual cognitions, psychology and/or behaviors at the center of public health conceptualizations of pregnancies, thereby leaving the underlying social and structural dynamics under-examined. I propose a model that places ‘supportability’ at the center of thinking about pregnancies and that allows for an analysis of the intersection of individual cognitions, emotions and behavior with micro-level interactive spaces and macro-level issues. , Full text access on Publisher website: https://academic.oup.com/jpubhealth/article-lookup/doi/10.1093/pubmed/fdv123
- Full Text: false
- Date Issued: 2015
- Authors: Macleod, Catriona I
- Date: 2015
- Language: English
- Type: Article
- Identifier: vital:6313 , http://hdl.handle.net/10962/d1019881 , https://academic.oup.com/jpubhealth/article-lookup/doi/10.1093/pubmed/fdv123
- Description: In this Perspectives paper, I outline the limitations of the concept of ‘intentionality’ in public reproductive health understandings of pregnancy. ‘Intentionality’, ‘plannedness’, ‘wantedness’ and ‘timing’ place individual cognitions, psychology and/or behaviors at the center of public health conceptualizations of pregnancies, thereby leaving the underlying social and structural dynamics under-examined. I propose a model that places ‘supportability’ at the center of thinking about pregnancies and that allows for an analysis of the intersection of individual cognitions, emotions and behavior with micro-level interactive spaces and macro-level issues. , Full text access on Publisher website: https://academic.oup.com/jpubhealth/article-lookup/doi/10.1093/pubmed/fdv123
- Full Text: false
- Date Issued: 2015
Electropolymerized Fluorinated Aniline-Based Fiber for Headspace Solid-Phase Microextraction and Gas Chromatographic Determination of Benzaldehyde in Injectable Pharmaceutical Formulations
- Mohammadi, Ali, Mohammadi, Somayeh, Moghaddam, Bayandori A, Masoumi, Vahideh, Walker, Roderick B
- Authors: Mohammadi, Ali , Mohammadi, Somayeh , Moghaddam, Bayandori A , Masoumi, Vahideh , Walker, Roderick B
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/184120 , vital:44175 , xlink:href="https://doi.org/10.1093/chromsci/bmt152"
- Description: In this study, a simple method was developed and validated to detect trace levels of benzaldehyde in injectable pharmaceutical formulations by solid-phase microextraction coupled with gas chromatography–flame ionization detector. Polyaniline was electrodeposited on a platinum wire in trifluoroacetic acid solvent by cyclic voltammetry technique. This fiber shows high thermal and mechanical stability and high performance in extraction of benzaldehyde. Extraction and desorption time and temperature, salt effect and gas chromatography parameters were optimized as key parameters. At the optimum conditions, the fiber shows good linearity between peak area ratio of benzaldehyde/3-chlorobenzaldehyde and benzaldehyde concentration in the range of 50–800 ng/mL with percent relative standard deviation values ranging from 0.75 to 8.64% (n 5 3). The limits of quantitation and detection were 50 and 16 ng/mL, respectively. The method has the requisite selectivity, sensitivity, accuracy and precision to assay benzaldehyde in injectable pharmaceutical dosage forms.
- Full Text:
- Date Issued: 2014
- Authors: Mohammadi, Ali , Mohammadi, Somayeh , Moghaddam, Bayandori A , Masoumi, Vahideh , Walker, Roderick B
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/184120 , vital:44175 , xlink:href="https://doi.org/10.1093/chromsci/bmt152"
- Description: In this study, a simple method was developed and validated to detect trace levels of benzaldehyde in injectable pharmaceutical formulations by solid-phase microextraction coupled with gas chromatography–flame ionization detector. Polyaniline was electrodeposited on a platinum wire in trifluoroacetic acid solvent by cyclic voltammetry technique. This fiber shows high thermal and mechanical stability and high performance in extraction of benzaldehyde. Extraction and desorption time and temperature, salt effect and gas chromatography parameters were optimized as key parameters. At the optimum conditions, the fiber shows good linearity between peak area ratio of benzaldehyde/3-chlorobenzaldehyde and benzaldehyde concentration in the range of 50–800 ng/mL with percent relative standard deviation values ranging from 0.75 to 8.64% (n 5 3). The limits of quantitation and detection were 50 and 16 ng/mL, respectively. The method has the requisite selectivity, sensitivity, accuracy and precision to assay benzaldehyde in injectable pharmaceutical dosage forms.
- Full Text:
- Date Issued: 2014
Elizabeth Costello and the Biography of the Moral Philosopher
- Authors: Jones, Ward E
- Date: 2011
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/275635 , vital:55065 , xlink:href="https://doi.org/10.1111/j.1540-6245.2011.01462.x"
- Description: Imagine someone who informs you that her conversion to vegetarianism began when she read Charlotte's Web or viewed the film Babe. Both stories invite the reader to celebrate the events surrounding a pig being saved from the butcher. What kind of role would her spectatorship of this book or film have played in her conversion? It is perhaps improbable to suspect that she would have undergone this kind of extreme moral conversion solely on the basis of her engagement with one of these fictions; perhaps more likely is the scenario in which her engagement was only one part of a lengthy process of her moral change of mind. In any event, it is certainly possible that our imagined vegetarian would see her encounter with Charlotte's Web or Babe as playing a justificatory role in her conversion. In looking back at her conversion, she might say something like this: “I know that I was young and impressionable, but the way in which the book (or film) made me feel about its characters moved me to further reflect upon animals and the animal industry, and I now realize that it was right to do so.” On her own view, at least, her spectatorship motivated and warranted her taking the further steps that ultimately led to her conversion. If she is right, then fictional narratives can possess—to at least some degree—what Raimond Gaita refers to as an ethical “authority.”
- Full Text:
- Date Issued: 2011
- Authors: Jones, Ward E
- Date: 2011
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/275635 , vital:55065 , xlink:href="https://doi.org/10.1111/j.1540-6245.2011.01462.x"
- Description: Imagine someone who informs you that her conversion to vegetarianism began when she read Charlotte's Web or viewed the film Babe. Both stories invite the reader to celebrate the events surrounding a pig being saved from the butcher. What kind of role would her spectatorship of this book or film have played in her conversion? It is perhaps improbable to suspect that she would have undergone this kind of extreme moral conversion solely on the basis of her engagement with one of these fictions; perhaps more likely is the scenario in which her engagement was only one part of a lengthy process of her moral change of mind. In any event, it is certainly possible that our imagined vegetarian would see her encounter with Charlotte's Web or Babe as playing a justificatory role in her conversion. In looking back at her conversion, she might say something like this: “I know that I was young and impressionable, but the way in which the book (or film) made me feel about its characters moved me to further reflect upon animals and the animal industry, and I now realize that it was right to do so.” On her own view, at least, her spectatorship motivated and warranted her taking the further steps that ultimately led to her conversion. If she is right, then fictional narratives can possess—to at least some degree—what Raimond Gaita refers to as an ethical “authority.”
- Full Text:
- Date Issued: 2011
Art and Ethical Criticism, edited by Garry L. Hagberg
- Authors: Jones, Ward E
- Date: 2010
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/275612 , vital:55063 , xlink:href="https://doi.org/10.1093/mind/fzq098"
- Description: Book Review: If there is a norm that a book’s title should give a reliable indication of its contents, Art and Ethical Criticism does not quite meet it. By my judgement, only seven of its twelve essays concern either the ethical criticism of art or the criticism of ethical positions within artworks.
- Full Text:
- Date Issued: 2010
- Authors: Jones, Ward E
- Date: 2010
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/275612 , vital:55063 , xlink:href="https://doi.org/10.1093/mind/fzq098"
- Description: Book Review: If there is a norm that a book’s title should give a reliable indication of its contents, Art and Ethical Criticism does not quite meet it. By my judgement, only seven of its twelve essays concern either the ethical criticism of art or the criticism of ethical positions within artworks.
- Full Text:
- Date Issued: 2010
Development and validation of a stability-indicating method for the quantitation of paclitaxel in pharmaceutical dosage forms
- Mohammadi, Ali, Esimaeili, Farnaz, Dinarvand, Rasoul, Atyabi, Fatemeh, Walker, Roderick B
- Authors: Mohammadi, Ali , Esimaeili, Farnaz , Dinarvand, Rasoul , Atyabi, Fatemeh , Walker, Roderick B
- Date: 2009
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/184278 , vital:44196 , xlink:href="https://doi.org/10.1093/chromsci/47.7.599"
- Description: A simple, rapid stability-indicating isocratic assay has been developed and validated for the determination of Paclitaxel (PTX) in commercial injection formulations. The assay is performed using a Nucleosil RP-18 (5 µm, 250 × 4.0 mm i.d) column protected by a Nucleosil C18 precolumn (5 µm, 4.0 × 4.0 mm i.d.) with a mobile phase of methanol–water (80:20) and UV detection at 230 nm. The method was found to be specific for PTX in the presence of degradation products with an overall analytical run time of ~ 9 min. Accuracy reported as % bias was found to be 0.1–2.5% bias for all samples tested. Intra-assay precision (repeatability) was found to be 0.22–2.65% RSD, while inter-day precision (intermediate precision) was found to be 1.0–3.0% RSD for the samples studied. The calibration curve was found to be linear with the equation y = 29.78x + 7.65, and a linear regression coefficient of 0.9994 over the concentration range 0.05–20 µg/mL. The limits of quantitation and detection were 0.05 and 0.02 µg/mL, respectively. Taxol (30 mg/5 mL), a commercially available dosage form of PTX, was assayed and 100.6–103.6% of the label claim was recovered.
- Full Text:
- Date Issued: 2009
- Authors: Mohammadi, Ali , Esimaeili, Farnaz , Dinarvand, Rasoul , Atyabi, Fatemeh , Walker, Roderick B
- Date: 2009
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/184278 , vital:44196 , xlink:href="https://doi.org/10.1093/chromsci/47.7.599"
- Description: A simple, rapid stability-indicating isocratic assay has been developed and validated for the determination of Paclitaxel (PTX) in commercial injection formulations. The assay is performed using a Nucleosil RP-18 (5 µm, 250 × 4.0 mm i.d) column protected by a Nucleosil C18 precolumn (5 µm, 4.0 × 4.0 mm i.d.) with a mobile phase of methanol–water (80:20) and UV detection at 230 nm. The method was found to be specific for PTX in the presence of degradation products with an overall analytical run time of ~ 9 min. Accuracy reported as % bias was found to be 0.1–2.5% bias for all samples tested. Intra-assay precision (repeatability) was found to be 0.22–2.65% RSD, while inter-day precision (intermediate precision) was found to be 1.0–3.0% RSD for the samples studied. The calibration curve was found to be linear with the equation y = 29.78x + 7.65, and a linear regression coefficient of 0.9994 over the concentration range 0.05–20 µg/mL. The limits of quantitation and detection were 0.05 and 0.02 µg/mL, respectively. Taxol (30 mg/5 mL), a commercially available dosage form of PTX, was assayed and 100.6–103.6% of the label claim was recovered.
- Full Text:
- Date Issued: 2009
A sensitive and reliable method for the detection of lipid peroxidation in biological tissues
- Anoopkumar-Dukie, Shailendra, Walker, Roderick B, Daya, Santylal
- Authors: Anoopkumar-Dukie, Shailendra , Walker, Roderick B , Daya, Santylal
- Date: 2001
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/184325 , vital:44208 , xlink:href="https://doi.org/10.1211/0022357011775299"
- Description: A simple, accurate and cost effective method has been designed for the determination of lipid peroxidation in biological tissue samples. The method was a modification and improvement on existing methods available for lipid peroxidation determination. Solid-phase extraction was used to separate the thiobarbituric acid–malondialdehyde complex from thiobarbituric acidreactive substances and HPLC was performed using a C18 (Waters Spherisorb, 5 µm, 250¬4.6 mm i.d.) column to achieve isolation of the complex. The procedure was validated with respect to linearity of calibration (0.998), precision, sensitivity and limits of quantitation (1 nmol mL−1) and detection (0.5 nmol mL−1). Resorcinol was used as an external standard. The method was tested by inducing free radical generation with a known free radical generator, quinolinic acid, in rat brain homogenate. The results showed that the method presented allowed detection of lipid peroxidation products at concentrations in the nanomolar (nM) range compared with the micromolar (µM) range detected by other methods, thus rendering it suitable for use with biological samples. In addition, the modified method allowed for detection of the purified lipid peroxidation products, thus eliminating the possibility of simultaneous detection of impurities that absorb at the same wavelength.
- Full Text:
- Date Issued: 2001
- Authors: Anoopkumar-Dukie, Shailendra , Walker, Roderick B , Daya, Santylal
- Date: 2001
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/184325 , vital:44208 , xlink:href="https://doi.org/10.1211/0022357011775299"
- Description: A simple, accurate and cost effective method has been designed for the determination of lipid peroxidation in biological tissue samples. The method was a modification and improvement on existing methods available for lipid peroxidation determination. Solid-phase extraction was used to separate the thiobarbituric acid–malondialdehyde complex from thiobarbituric acidreactive substances and HPLC was performed using a C18 (Waters Spherisorb, 5 µm, 250¬4.6 mm i.d.) column to achieve isolation of the complex. The procedure was validated with respect to linearity of calibration (0.998), precision, sensitivity and limits of quantitation (1 nmol mL−1) and detection (0.5 nmol mL−1). Resorcinol was used as an external standard. The method was tested by inducing free radical generation with a known free radical generator, quinolinic acid, in rat brain homogenate. The results showed that the method presented allowed detection of lipid peroxidation products at concentrations in the nanomolar (nM) range compared with the micromolar (µM) range detected by other methods, thus rendering it suitable for use with biological samples. In addition, the modified method allowed for detection of the purified lipid peroxidation products, thus eliminating the possibility of simultaneous detection of impurities that absorb at the same wavelength.
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- Date Issued: 2001
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