An investigation of absenteeism amongst blue-collar workers: a mixed methods approach
- Authors: Kroon, Kim
- Date: 2016
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:866 , http://hdl.handle.net/10962/d1021248
- Description: Absenteeism affects organisations in many different ways, therefore it is essential to investigate absenteeism with the aim of minimising absence levels. The focus of the research was to investigate absenteeism amongst blue-collar workers at a Global Logistics Supplier. The objectives of this research were to identify the main types of absenteeism, to compare the views of blue-collar workers and management on the causes attributed to absenteeism, to describe how absenteeism has been measured and managed, and to make recommendations to the company on ways to minimize absenteeism based on the findings. Quantitative data in the form of monthly absenteeism statistics relating to each leave type, was examined for trends relating to absenteeism levels amongst blue-collar workers at the company. Semi-structured interviews were then carried out with management and blue-collar workers to examine the causes of absenteeism and its management. The results showed that there was a discrepancy between the views of management and blue-collar workers at the company on the perceived causes of absenteeism. On the other hand, both management and the blue-collar workers identified Monday and Friday as the most prevalent days on which employees are absent from the workplace. This finding suggests that more research should be conducted on this trend in blue-collar workers. In exploring how absenteeism is dealt with, management within the company stated that discipline was the most effective way of keeping absenteeism levels low amongst employees. The limitations and delimitations of the research were recognised and further research ideas were given. It was also recommended that managers and supervisors address the workplace issues and identify when a worker’s performance and interpersonal relationships are strained in order to effectively minimise absenteeism. It is also recommended they implement a wellness programme.
- Full Text:
- Authors: Kroon, Kim
- Date: 2016
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:866 , http://hdl.handle.net/10962/d1021248
- Description: Absenteeism affects organisations in many different ways, therefore it is essential to investigate absenteeism with the aim of minimising absence levels. The focus of the research was to investigate absenteeism amongst blue-collar workers at a Global Logistics Supplier. The objectives of this research were to identify the main types of absenteeism, to compare the views of blue-collar workers and management on the causes attributed to absenteeism, to describe how absenteeism has been measured and managed, and to make recommendations to the company on ways to minimize absenteeism based on the findings. Quantitative data in the form of monthly absenteeism statistics relating to each leave type, was examined for trends relating to absenteeism levels amongst blue-collar workers at the company. Semi-structured interviews were then carried out with management and blue-collar workers to examine the causes of absenteeism and its management. The results showed that there was a discrepancy between the views of management and blue-collar workers at the company on the perceived causes of absenteeism. On the other hand, both management and the blue-collar workers identified Monday and Friday as the most prevalent days on which employees are absent from the workplace. This finding suggests that more research should be conducted on this trend in blue-collar workers. In exploring how absenteeism is dealt with, management within the company stated that discipline was the most effective way of keeping absenteeism levels low amongst employees. The limitations and delimitations of the research were recognised and further research ideas were given. It was also recommended that managers and supervisors address the workplace issues and identify when a worker’s performance and interpersonal relationships are strained in order to effectively minimise absenteeism. It is also recommended they implement a wellness programme.
- Full Text:
An investigation of green marketing opportunities at BOS Ice Tea
- Authors: Ciesielski, Adrian Adam
- Date: 2016
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:864 , http://hdl.handle.net/10962/d1021246
- Description: The research addresses the marketing activities of BOS Ice Tea, a South African company, and whether they are reflective and grounded within their marketing activities. By analysing public data and interviewing the founding partners, Richard Bowsher and Grant Rushmere, the findings where compared against the marketing paradigms of Ottoman (1999) and the green marketing practices of Gittell et al. (2012). It was determined that the company engages in green activities, but does not communicate them clearly. Further, where the company did promote organic rooibos and sustainability, their actions were not reflective of these behaviours from an academic point of view. Thus, there was the potential for green washing. The research concludes with suggestions for a 4P’s green marketing framework, and comments on areas that need clarity.
- Full Text:
- Authors: Ciesielski, Adrian Adam
- Date: 2016
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:864 , http://hdl.handle.net/10962/d1021246
- Description: The research addresses the marketing activities of BOS Ice Tea, a South African company, and whether they are reflective and grounded within their marketing activities. By analysing public data and interviewing the founding partners, Richard Bowsher and Grant Rushmere, the findings where compared against the marketing paradigms of Ottoman (1999) and the green marketing practices of Gittell et al. (2012). It was determined that the company engages in green activities, but does not communicate them clearly. Further, where the company did promote organic rooibos and sustainability, their actions were not reflective of these behaviours from an academic point of view. Thus, there was the potential for green washing. The research concludes with suggestions for a 4P’s green marketing framework, and comments on areas that need clarity.
- Full Text:
An investigation of participative management in a museum in the Eastern Cape, South Africa
- Authors: Madinda, Nozipho
- Date: 2016
- Subjects: Museums -- South Africa -- Eastern Cape -- Management , Management -- Employee participation , Albany Museum (Grahamstown, South Africa)
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:2068 , http://hdl.handle.net/10962/d1020847
- Description: The purpose of my research was to investigate participative management at the Albany Museum with a view to generating knowledge and insights that can be used to support senior management’s engagement with participative management at mid-management level. My interest was to investigate participative management with regards to five HODs of the Albany Museum with a view to generating knowledge and insights that can be used to support senior management’s engagement with participative management at mid-management level. The research was informed by the interpretive paradigm. The interpretive paradigm does not concern itself with the search for broadly applicable laws and rules but rather seeks to produce descriptive analyses that emphasise deep interpretation and understanding of social phenomena through the meaning that the people assign to them. This study is mostly descriptive and presents the reality of participants from their own experience. Semi-structured interviews and observation capture ‘insider’ knowledge that is part of an interpretive methodology. The study found that participative management was both understood and generally accepted as a good way to manage an organisation, and even members who were critical of it could see its benefits. However, the fractured and diversified structure of the organisation calls for a particularly skillful application of this management approach, one which would also demand leadership and a greater sense of working towards what are called collegial models of management. Whether this is in fact desirable for a museum is debatable.
- Full Text:
- Authors: Madinda, Nozipho
- Date: 2016
- Subjects: Museums -- South Africa -- Eastern Cape -- Management , Management -- Employee participation , Albany Museum (Grahamstown, South Africa)
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:2068 , http://hdl.handle.net/10962/d1020847
- Description: The purpose of my research was to investigate participative management at the Albany Museum with a view to generating knowledge and insights that can be used to support senior management’s engagement with participative management at mid-management level. My interest was to investigate participative management with regards to five HODs of the Albany Museum with a view to generating knowledge and insights that can be used to support senior management’s engagement with participative management at mid-management level. The research was informed by the interpretive paradigm. The interpretive paradigm does not concern itself with the search for broadly applicable laws and rules but rather seeks to produce descriptive analyses that emphasise deep interpretation and understanding of social phenomena through the meaning that the people assign to them. This study is mostly descriptive and presents the reality of participants from their own experience. Semi-structured interviews and observation capture ‘insider’ knowledge that is part of an interpretive methodology. The study found that participative management was both understood and generally accepted as a good way to manage an organisation, and even members who were critical of it could see its benefits. However, the fractured and diversified structure of the organisation calls for a particularly skillful application of this management approach, one which would also demand leadership and a greater sense of working towards what are called collegial models of management. Whether this is in fact desirable for a museum is debatable.
- Full Text:
An investigation of servant leadership within Rhodes University sports clubs
- Authors: Stiebel, Jessica Lee
- Date: 2016
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:872 , http://hdl.handle.net/10962/d1021317
- Description: This research investigates the level of servant leadership within Rhodes University sport clubs. The goals of this research are to assess the perception the club members have of their chairperson and to key identify areas of development. The sport clubs at Rhodes University are partially run by chairpersons who are voted into the position by his or her peers of a sports club. The chairpersons work alongside Rhodes University employees who are a part of the Sports Administration team to oversee the running, scheduling and finances of the club. The sports clubs are representative of Rhodes University and it is the chairperson’s responsibility to ensure the club is lead and run effectively. Robert Greenleaf coined the term servant leader in the 1970s (Greenleaf, 1997). The servant leader is a leader who is driven by his or her followers, as leadership involves a leader serving his or her followers first and foremost (Greenleaf, 1977). The leader takes on a role that is supportive and contributes to their personal as well as their professional achievements (Smith, Montagno and Kuzmenko, 2004). A questionnaire consisting of 27 questions was drafted, of which were four demographic questions. A 5-point Likert Scale was used in order for the participants to respond to the questions. The questionnaire was drawn from a number of questionnaires available in literature on leadership and adapted to suit Rhodes University sports clubs. The questionnaire was distributed personally by the researcher, as well as through an online questionnaire. There were a total of 153 candidates who participated in the study. Descriptive statistics were used to analyse the data. The majority of the respondents had the perceptions that their chairperson displayed characteristics of a servant leader. Majority of the participants were in agreement of the questions asked in the questionnaire, which focused on many servant leadership qualities. The results will be handed over to the Head of Sports Administration in order for them to get an idea of their leaders’ characteristics and areas where they can improve upon.
- Full Text:
- Authors: Stiebel, Jessica Lee
- Date: 2016
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:872 , http://hdl.handle.net/10962/d1021317
- Description: This research investigates the level of servant leadership within Rhodes University sport clubs. The goals of this research are to assess the perception the club members have of their chairperson and to key identify areas of development. The sport clubs at Rhodes University are partially run by chairpersons who are voted into the position by his or her peers of a sports club. The chairpersons work alongside Rhodes University employees who are a part of the Sports Administration team to oversee the running, scheduling and finances of the club. The sports clubs are representative of Rhodes University and it is the chairperson’s responsibility to ensure the club is lead and run effectively. Robert Greenleaf coined the term servant leader in the 1970s (Greenleaf, 1997). The servant leader is a leader who is driven by his or her followers, as leadership involves a leader serving his or her followers first and foremost (Greenleaf, 1977). The leader takes on a role that is supportive and contributes to their personal as well as their professional achievements (Smith, Montagno and Kuzmenko, 2004). A questionnaire consisting of 27 questions was drafted, of which were four demographic questions. A 5-point Likert Scale was used in order for the participants to respond to the questions. The questionnaire was drawn from a number of questionnaires available in literature on leadership and adapted to suit Rhodes University sports clubs. The questionnaire was distributed personally by the researcher, as well as through an online questionnaire. There were a total of 153 candidates who participated in the study. Descriptive statistics were used to analyse the data. The majority of the respondents had the perceptions that their chairperson displayed characteristics of a servant leader. Majority of the participants were in agreement of the questions asked in the questionnaire, which focused on many servant leadership qualities. The results will be handed over to the Head of Sports Administration in order for them to get an idea of their leaders’ characteristics and areas where they can improve upon.
- Full Text:
An investigation of the aquatic macroinvertebrate fauna of the southern Great Escarpment (South Africa): insights from ecological and genetic studies
- Authors: Taylor, Chantal Lee
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4275 , http://hdl.handle.net/10962/d1020839
- Description: Biological diversity in freshwater biomes is vital to maintain healthy, functioning ecosystems with resilience to disturbance and the impacts of climate change. Freshwater ecosystems provide essential resources to life on Earth. However, as increasing pressure is being placed on the environment by human population growth, the quality of freshwater resources and the health of these ecosystems are at risk. Mountain streams provide an important source of water and are usually less affected by anthropogenic stressors, compared to lowland freshwaters. These montane streams are therefore of important conservation value and due to their untransformed nature serve as ideal ecosystems for biodiversity studies and as reference sites for studies on environmental change. This study explores aquatic macroinvertebrate biodiversity of the southern Great Escarpment in South Africa. Species assemblages and the environmental variables of each site were sampled from first order streams across five different mountain blocks along the Great Escarpment. Additionally, mitochondrial DNA of three mayfly species (Afroptilum sudafricanum, Demoreptus natalensis and Demoreptus capensis), commonly occurring in the study area, was analysed to compare the genetic diversity between habitat specialist and habitat generalist species. A total of 2 595 macroinvertebrate specimens from 47 families and 86 species were collected with several interesting and potentially new species being discovered. Partitioned diversity analyses indicate that macroinvertebrate diversity varies across mountain blocks. Multivariate analyses indicate that differences in assemblages could be attributed to differences in environmental variables between sites, particularly water velocity, total dissolved solids and salinity. As these environmental variables reflect of the topography of the sites, differences in species assemblages was attributed to difference in topography and therefore biotopes present. Habitat-restricted mayfly species (D. natalensis and D. capensis), occur in distinct populations confined to mountains blocks. Isolation-by-distance analyses further emphasis that these species are genetically restricted by their habitat preference for mountain streams. In contrast, A. sudafricanum, a habitat generalist, showed no indication of genetic structure due to location or distance. Possible cryptic taxa and new species were identified within A. sudafricanum and Dermoreptus respectively. This study provides an important contribution to the baseline data of freshwater macroinvertebrate diversity for the southern Great Escarpment region and provides insights into the considerable genetic diversity of selected aquatic taxa across this region.
- Full Text:
- Authors: Taylor, Chantal Lee
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4275 , http://hdl.handle.net/10962/d1020839
- Description: Biological diversity in freshwater biomes is vital to maintain healthy, functioning ecosystems with resilience to disturbance and the impacts of climate change. Freshwater ecosystems provide essential resources to life on Earth. However, as increasing pressure is being placed on the environment by human population growth, the quality of freshwater resources and the health of these ecosystems are at risk. Mountain streams provide an important source of water and are usually less affected by anthropogenic stressors, compared to lowland freshwaters. These montane streams are therefore of important conservation value and due to their untransformed nature serve as ideal ecosystems for biodiversity studies and as reference sites for studies on environmental change. This study explores aquatic macroinvertebrate biodiversity of the southern Great Escarpment in South Africa. Species assemblages and the environmental variables of each site were sampled from first order streams across five different mountain blocks along the Great Escarpment. Additionally, mitochondrial DNA of three mayfly species (Afroptilum sudafricanum, Demoreptus natalensis and Demoreptus capensis), commonly occurring in the study area, was analysed to compare the genetic diversity between habitat specialist and habitat generalist species. A total of 2 595 macroinvertebrate specimens from 47 families and 86 species were collected with several interesting and potentially new species being discovered. Partitioned diversity analyses indicate that macroinvertebrate diversity varies across mountain blocks. Multivariate analyses indicate that differences in assemblages could be attributed to differences in environmental variables between sites, particularly water velocity, total dissolved solids and salinity. As these environmental variables reflect of the topography of the sites, differences in species assemblages was attributed to difference in topography and therefore biotopes present. Habitat-restricted mayfly species (D. natalensis and D. capensis), occur in distinct populations confined to mountains blocks. Isolation-by-distance analyses further emphasis that these species are genetically restricted by their habitat preference for mountain streams. In contrast, A. sudafricanum, a habitat generalist, showed no indication of genetic structure due to location or distance. Possible cryptic taxa and new species were identified within A. sudafricanum and Dermoreptus respectively. This study provides an important contribution to the baseline data of freshwater macroinvertebrate diversity for the southern Great Escarpment region and provides insights into the considerable genetic diversity of selected aquatic taxa across this region.
- Full Text:
An investigation of the barriers and drivers to CDM renewable energy investment at the Metro, Kouga and Jeffreys Bay windfarms
- Authors: Staples, Daryl Nolan
- Date: 2016
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:871 , http://hdl.handle.net/10962/d1021316
- Description: This research is presented in three sections. Section 1 presents the research report in an academic journal article format. Section 2 provides a comprehensive literature review and Section 3 describes the research methodology and methods employed during the research. Climate change is the most urgent challenge facing planet earth today. The Intergovernmental Panel on Climate Change (IPCC) fifth assessment report (AR5) clearly states, “Human influence on the climate system is clear, and recent anthropogenic emissions of greenhouse gases are the highest in history. Recent climate changes have had widespread impacts on human and natural systems” (IPCC, 2014:2). The Renewable Energy Policy Network for the 21st Century (REN21) published the 10th annual edition of the Renewables 2015 Global Status Report, which illustrates the importance of a zero emissions energy sector (REN21, 2015). Despite the world’s average annual 1,5% increase in energy consumption in recent years, and average 3% growth in Gross Domestic Product, carbon dioxide (CO2) emissions in 2014 were unchanged from 2013 levels (REN21, 2015). The report findings state, “For the first time in four decades, the world economy grew without a parallel rise in CO2 emissions” (REN21, 2015:17). Renewable energy and improved energy efficiency is key to limiting global warming and avoiding dangerous impacts from climate change (REN21, 2015). With a view to the successful outcome of the 21st Conference of the Parties (COP21) in December 2015, renewable energy could contribute significantly in mitigating climate change and supporting the Sustainable Development Goals (SDGs), specifically SDG 7, on Sustainable Energy for All (SAIREC, 2015). South Africa has been rated the 15th most attractive destination for investment in the renewable energy sector and could become a renewable energy leader in the African continent, according to the Renewable Energy Country Attractiveness Index of EY (Ernest and Young) (EY, 2015). And more...
- Full Text:
- Authors: Staples, Daryl Nolan
- Date: 2016
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:871 , http://hdl.handle.net/10962/d1021316
- Description: This research is presented in three sections. Section 1 presents the research report in an academic journal article format. Section 2 provides a comprehensive literature review and Section 3 describes the research methodology and methods employed during the research. Climate change is the most urgent challenge facing planet earth today. The Intergovernmental Panel on Climate Change (IPCC) fifth assessment report (AR5) clearly states, “Human influence on the climate system is clear, and recent anthropogenic emissions of greenhouse gases are the highest in history. Recent climate changes have had widespread impacts on human and natural systems” (IPCC, 2014:2). The Renewable Energy Policy Network for the 21st Century (REN21) published the 10th annual edition of the Renewables 2015 Global Status Report, which illustrates the importance of a zero emissions energy sector (REN21, 2015). Despite the world’s average annual 1,5% increase in energy consumption in recent years, and average 3% growth in Gross Domestic Product, carbon dioxide (CO2) emissions in 2014 were unchanged from 2013 levels (REN21, 2015). The report findings state, “For the first time in four decades, the world economy grew without a parallel rise in CO2 emissions” (REN21, 2015:17). Renewable energy and improved energy efficiency is key to limiting global warming and avoiding dangerous impacts from climate change (REN21, 2015). With a view to the successful outcome of the 21st Conference of the Parties (COP21) in December 2015, renewable energy could contribute significantly in mitigating climate change and supporting the Sustainable Development Goals (SDGs), specifically SDG 7, on Sustainable Energy for All (SAIREC, 2015). South Africa has been rated the 15th most attractive destination for investment in the renewable energy sector and could become a renewable energy leader in the African continent, according to the Renewable Energy Country Attractiveness Index of EY (Ernest and Young) (EY, 2015). And more...
- Full Text:
An investigation of the practices employed by an environmental community-based organization to successfully sustain its school based and community based projects (A case study)
- Authors: Hlophe, Nomalanga Nokuthula
- Date: 2016
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:2073 , http://hdl.handle.net/10962/d1021252
- Description: Community-based organizations (CBOs) play a crucial role in sustainable development and hence it is important that they are promoted, guided and supported by state agencies and the private sector. The South African government encourages communities to establish co-operatives as a tool or strategy to address local social issues and risks and act accordingly. The purpose of this case study was to determine what aspects of the establishment and operation of a successful community-based environmental organization are producing sustained school and community projects. The study set out to investigate and audit the activities of a successful environmental CBO so as to determine how it has successfully sustained its school and community environmental projects. The reason for this investigation was to inform other CBOs and the state environmental agencies that support them on how to sustain their environmental activities in community and school contexts. The investigation was designed as an interpretive case study, which used document analysis, semi-structured interviews and observations to gather data. The gathered data was analyzed through inductive analysis to interpret and audit reported activities. Analytical memos were used to represent key themes in relation to the successful operations of the organization. Through auditing and reporting the activities in the analytical memos, analytical statements were developed. Those statements guided the discussion and informed the study‟s findings and recommendations. After investigating this CBO, it was concluded that, their success is a result of the establishment of a networking forum with different stakeholders and parties, community involvement in different projects, partnerships with local schools to develop and expand their curriculum practice, CBO networking locally and internationally and finally, their participation in annual and continuous environmental competitions/projects/programs. The insights gained and lessons learned will be used to advice and support community based co-operatives in environmental learning activities in school and community contexts as part of my ongoing work.
- Full Text:
- Authors: Hlophe, Nomalanga Nokuthula
- Date: 2016
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:2073 , http://hdl.handle.net/10962/d1021252
- Description: Community-based organizations (CBOs) play a crucial role in sustainable development and hence it is important that they are promoted, guided and supported by state agencies and the private sector. The South African government encourages communities to establish co-operatives as a tool or strategy to address local social issues and risks and act accordingly. The purpose of this case study was to determine what aspects of the establishment and operation of a successful community-based environmental organization are producing sustained school and community projects. The study set out to investigate and audit the activities of a successful environmental CBO so as to determine how it has successfully sustained its school and community environmental projects. The reason for this investigation was to inform other CBOs and the state environmental agencies that support them on how to sustain their environmental activities in community and school contexts. The investigation was designed as an interpretive case study, which used document analysis, semi-structured interviews and observations to gather data. The gathered data was analyzed through inductive analysis to interpret and audit reported activities. Analytical memos were used to represent key themes in relation to the successful operations of the organization. Through auditing and reporting the activities in the analytical memos, analytical statements were developed. Those statements guided the discussion and informed the study‟s findings and recommendations. After investigating this CBO, it was concluded that, their success is a result of the establishment of a networking forum with different stakeholders and parties, community involvement in different projects, partnerships with local schools to develop and expand their curriculum practice, CBO networking locally and internationally and finally, their participation in annual and continuous environmental competitions/projects/programs. The insights gained and lessons learned will be used to advice and support community based co-operatives in environmental learning activities in school and community contexts as part of my ongoing work.
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An investigation on the effect of Russian wheat aphid (Diuraphis noxia Kurdjumov) population growth and feeding damage on selected barley (Hordeum vulgare L.) cultivars under ambient and elevated CO2
- Authors: Sacranie, Sattar Farouk
- Date: 2016
- Subjects: Russian wheat aphid -- Research -- South Africa Barley -- Disease and pest resistance -- South Africa Callose Aphids
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/50311 , vital:25975
- Description: The Russian wheat aphid (RWA) (Diuraphis noxia Kurdjumov) is a major pest of cultivated small grains. It is particularly devastating because of is high reproductive rate which results in the growth of large populations which become damaging to its host plants. Development of resistant barley (Hordeum vulgare L.) cultivars is complicated as resistance is polygenic. As a result, the industry remains at risk now that the RWA has spread throughout South Africa. It has, as recently as, 2013, been identified in the SW Cape, which was previously geographically isolated. This is South Africa‟s principle barley growing region. Now a potentially huge problem exists. Therefore, it is imperative that an alternative to pesticide use is found. Testing potential innate resistance in barley cultivars is thus, critical. In this thesis, I present data on four barely cultivars where I have examined their resistance/ lack of resistance to three known RWA biotypes, RWASA1, RWASA2 and RWASA3. The barley varieties used were two economically important South African malt barley cultivars (S5 and SSG 564) along with two potentially RWA resistant Afghan accessions (CIho 4125 and CIho 4159). The RWA biotype population growth rates on each of the plants were determined over a 14 day period. The aim was to establish baseline data of the effects of RWA population growth on the host plants under ambient CO2 (380 – 400 ppm) conditions. The extent of RWA feeding damage was investigated at the cell level by examining saliva deposition and cell disruption using Transmission Electron Microscopy; at the tissue/vascular level using fluorescence microscopy, to determine the extent of callose formation; at a whole leaf level by recording percent chlorosis and leaf roll; and finally, at a whole plant level by measuring biomass loss.The experiments were repeated under elevated CO2 (450 ppm) to model any changes in RWA/plant interaction with respect to future climate change. The effects of an elevated CO2 environment and RWA feeding on host plant foliar N and C:N ratio were compared to ambient CO2 conditions, to provide a clearer picture of the potential nutrient drain that a feeding RWA colony exacts on its host. Of the varieties tested, the CIho accessions performed better than the two SA barley cultivars as the CIho accessions appeared to express a mild antibiosis resistance response as RWA populations, particularly those of RWASA1, were smaller than those observed on either S5 or SSG 564. In addition, less damage was evident in the two CIho accessions due to RWA feeding. II RWASA2 was the most virulent of the three RWA biotypes tested, followed by RWASA3 while RWASA1 was the least virulent. Under elevated CO2 conditions, RWA feeding damage was exacerbated but the trend of biotype virulence remained the same. Higher aphid population sizes were recorded under elevated CO2, meant that even the more resistant CIho accessions were overcome by the increased demand made by the larger aphid colonies on the host plants. The % foliar N data showed that under elevated CO2 aphid-free control plants had increased N levels in their leaves. Increased “food” supply (as shown by the increased N levels) therefore allowed significantly larger aphid populations to develop on the plants exposed to elevated CO2, due to improved nutrient status of the phloem sap taken up by RWA. The knock-on effect of a higher aphid population was increased cell disruption as a result of extensive probing, extensive formations of wound callose, with the result that phloem damage impeded nutrient flow through the vascular tissues which contributed to chlorosis and (eventually plant) death. The major conclusion from this study is that even a mild CO2 elevation resulted in an increase aphid population which may pose a severe and very real threat to a barley crop. Therefore, without effort to identify and deploy resistant barley cultivars, it could well be possible that future barley cultivation in South Africa may no longer be viable.
- Full Text:
- Authors: Sacranie, Sattar Farouk
- Date: 2016
- Subjects: Russian wheat aphid -- Research -- South Africa Barley -- Disease and pest resistance -- South Africa Callose Aphids
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/50311 , vital:25975
- Description: The Russian wheat aphid (RWA) (Diuraphis noxia Kurdjumov) is a major pest of cultivated small grains. It is particularly devastating because of is high reproductive rate which results in the growth of large populations which become damaging to its host plants. Development of resistant barley (Hordeum vulgare L.) cultivars is complicated as resistance is polygenic. As a result, the industry remains at risk now that the RWA has spread throughout South Africa. It has, as recently as, 2013, been identified in the SW Cape, which was previously geographically isolated. This is South Africa‟s principle barley growing region. Now a potentially huge problem exists. Therefore, it is imperative that an alternative to pesticide use is found. Testing potential innate resistance in barley cultivars is thus, critical. In this thesis, I present data on four barely cultivars where I have examined their resistance/ lack of resistance to three known RWA biotypes, RWASA1, RWASA2 and RWASA3. The barley varieties used were two economically important South African malt barley cultivars (S5 and SSG 564) along with two potentially RWA resistant Afghan accessions (CIho 4125 and CIho 4159). The RWA biotype population growth rates on each of the plants were determined over a 14 day period. The aim was to establish baseline data of the effects of RWA population growth on the host plants under ambient CO2 (380 – 400 ppm) conditions. The extent of RWA feeding damage was investigated at the cell level by examining saliva deposition and cell disruption using Transmission Electron Microscopy; at the tissue/vascular level using fluorescence microscopy, to determine the extent of callose formation; at a whole leaf level by recording percent chlorosis and leaf roll; and finally, at a whole plant level by measuring biomass loss.The experiments were repeated under elevated CO2 (450 ppm) to model any changes in RWA/plant interaction with respect to future climate change. The effects of an elevated CO2 environment and RWA feeding on host plant foliar N and C:N ratio were compared to ambient CO2 conditions, to provide a clearer picture of the potential nutrient drain that a feeding RWA colony exacts on its host. Of the varieties tested, the CIho accessions performed better than the two SA barley cultivars as the CIho accessions appeared to express a mild antibiosis resistance response as RWA populations, particularly those of RWASA1, were smaller than those observed on either S5 or SSG 564. In addition, less damage was evident in the two CIho accessions due to RWA feeding. II RWASA2 was the most virulent of the three RWA biotypes tested, followed by RWASA3 while RWASA1 was the least virulent. Under elevated CO2 conditions, RWA feeding damage was exacerbated but the trend of biotype virulence remained the same. Higher aphid population sizes were recorded under elevated CO2, meant that even the more resistant CIho accessions were overcome by the increased demand made by the larger aphid colonies on the host plants. The % foliar N data showed that under elevated CO2 aphid-free control plants had increased N levels in their leaves. Increased “food” supply (as shown by the increased N levels) therefore allowed significantly larger aphid populations to develop on the plants exposed to elevated CO2, due to improved nutrient status of the phloem sap taken up by RWA. The knock-on effect of a higher aphid population was increased cell disruption as a result of extensive probing, extensive formations of wound callose, with the result that phloem damage impeded nutrient flow through the vascular tissues which contributed to chlorosis and (eventually plant) death. The major conclusion from this study is that even a mild CO2 elevation resulted in an increase aphid population which may pose a severe and very real threat to a barley crop. Therefore, without effort to identify and deploy resistant barley cultivars, it could well be possible that future barley cultivation in South Africa may no longer be viable.
- Full Text:
Assessing estuarine nursery habitats for Cape Stumpnose (Rhabdosargus holubi), (Pisces: Sparidae) in a warm-temperate estuary in the Eastern Cape, South Africa
- Authors: Leslie, Timothy David
- Date: 2016
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3178 , vital:20381
- Description: The nursery role hypothesis provides an approach for assessing the nursery function of habitat types within estuaries. This study attempted to assess the nursery value of the dominant estuarine habitats in the Bushmans Estuary for Rhabdosargus holubi (Steindachner 1881) by analysing habitat complexity, relative abundance and behaviour of R. holubi and using stomach contents analysis and dietary diatom composition as indicative of feeding habitat. Structural habitat complexity was assessed in Zostera capensis (Setchell) seagrass and Spartina maritima (Curtis Fernald) salt marsh by sampling above-ground stem density and length, and total cover per unit area (Ct/At). Dimensionless habitat complexity indices such as the interstitial spatial index (ISI) at three magnifications and fractal geometry at two magnifications were used to further analyse habitat complexity. Above-ground biomass (P<0.05) in each season and canopy height (P<0.001) were significantly higher in salt marsh than in seagrass whilst stem density was significantly higher in seagrass than in salt marsh in each season (P<0.001). Each dimensionless index indicated that complexity is notably higher in seagrass than in the salt marsh. Using dimensionless indices that analyse complexity at different spatial scales provided a better analysis of habitat complexity than canopy height and biomass as it allowed for direct comparisons between habitat types. Underwater video cameras were deployed in seagrass, salt marsh and sand flat habitats to assess the relative abundance and behaviour of R. holubi. The relative abundance of R. holubi was significantly higher in seagrass than salt marsh and sand flats, whilst the behaviour of R. holubi indicated a high degree of habitat use in structured habitats and a low degree of habitat use in unstructured sand flat habitats. This indicated that not only are juvenile R. holubi a vegetation-associated species, but also a species that prefers seagrass to salt marsh.
- Full Text:
- Authors: Leslie, Timothy David
- Date: 2016
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3178 , vital:20381
- Description: The nursery role hypothesis provides an approach for assessing the nursery function of habitat types within estuaries. This study attempted to assess the nursery value of the dominant estuarine habitats in the Bushmans Estuary for Rhabdosargus holubi (Steindachner 1881) by analysing habitat complexity, relative abundance and behaviour of R. holubi and using stomach contents analysis and dietary diatom composition as indicative of feeding habitat. Structural habitat complexity was assessed in Zostera capensis (Setchell) seagrass and Spartina maritima (Curtis Fernald) salt marsh by sampling above-ground stem density and length, and total cover per unit area (Ct/At). Dimensionless habitat complexity indices such as the interstitial spatial index (ISI) at three magnifications and fractal geometry at two magnifications were used to further analyse habitat complexity. Above-ground biomass (P<0.05) in each season and canopy height (P<0.001) were significantly higher in salt marsh than in seagrass whilst stem density was significantly higher in seagrass than in salt marsh in each season (P<0.001). Each dimensionless index indicated that complexity is notably higher in seagrass than in the salt marsh. Using dimensionless indices that analyse complexity at different spatial scales provided a better analysis of habitat complexity than canopy height and biomass as it allowed for direct comparisons between habitat types. Underwater video cameras were deployed in seagrass, salt marsh and sand flat habitats to assess the relative abundance and behaviour of R. holubi. The relative abundance of R. holubi was significantly higher in seagrass than salt marsh and sand flats, whilst the behaviour of R. holubi indicated a high degree of habitat use in structured habitats and a low degree of habitat use in unstructured sand flat habitats. This indicated that not only are juvenile R. holubi a vegetation-associated species, but also a species that prefers seagrass to salt marsh.
- Full Text:
Assessing the value of public investment into biological control research for invasive alien plants : the ARC PPRI Weeds Research Division
- Authors: Scarr, Lowell Martin
- Date: 2016
- Subjects: Alien plants -- Biological control , Invasive plants -- Biological control , Alien plants -- Economic aspects , Invasive plants -- Economic aspects , Weeds -- Biological control
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1126 , http://hdl.handle.net/10962/d1020604
- Description: This study investigates the economic impact of the ARC PPRI Weeds Research Division. The Division researches appropriate methods of biological control for invasive alien plants (IAPs). These plants pose an increasing threat to environmental integrity and ecosystem service provision impacting on economic potential. Since the work of the Division is considered a public good, a predominantly descriptive approach has been adopted for the valuation process. A combination of quantitative cost analysis and a qualitative study of the impacts of research and invasive alien plants is used to deal with the challenges associated with non-market valuation. The study found that investment into the Weeds Division is a valuable activity that supports the long-term growth potential of the South African economy. The role of a well-functioning environment is highlighted as an essential base for the creation of sustained growth opportunities in any society. It was determined that investment into the Division should be increased into the future to support efficient spending of scarce state funds. Biological control research was found to provide strategic future growth potential, creating opportunities for the development of a competitive advantage in the biotechnology and environmental management sectors. The study adds to the increasing move towards a more holistic view of economic valuation, taking factors other than pure finance and econometrics into consideration. This is an important shift in prevailing economic thought, as a realisation is reached that a single, or even triple, bottom line is an outdated and insufficient decision making basis.
- Full Text:
- Authors: Scarr, Lowell Martin
- Date: 2016
- Subjects: Alien plants -- Biological control , Invasive plants -- Biological control , Alien plants -- Economic aspects , Invasive plants -- Economic aspects , Weeds -- Biological control
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1126 , http://hdl.handle.net/10962/d1020604
- Description: This study investigates the economic impact of the ARC PPRI Weeds Research Division. The Division researches appropriate methods of biological control for invasive alien plants (IAPs). These plants pose an increasing threat to environmental integrity and ecosystem service provision impacting on economic potential. Since the work of the Division is considered a public good, a predominantly descriptive approach has been adopted for the valuation process. A combination of quantitative cost analysis and a qualitative study of the impacts of research and invasive alien plants is used to deal with the challenges associated with non-market valuation. The study found that investment into the Weeds Division is a valuable activity that supports the long-term growth potential of the South African economy. The role of a well-functioning environment is highlighted as an essential base for the creation of sustained growth opportunities in any society. It was determined that investment into the Division should be increased into the future to support efficient spending of scarce state funds. Biological control research was found to provide strategic future growth potential, creating opportunities for the development of a competitive advantage in the biotechnology and environmental management sectors. The study adds to the increasing move towards a more holistic view of economic valuation, taking factors other than pure finance and econometrics into consideration. This is an important shift in prevailing economic thought, as a realisation is reached that a single, or even triple, bottom line is an outdated and insufficient decision making basis.
- Full Text:
Bayesian accelerated life tests: exponential and Weibull models
- Authors: Izally, Sharkay Ruwade
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3003 , vital:20351
- Description: Reliability life testing is used for life data analysis in which samples are tested under normal conditions to obtain failure time data for reliability assessment. It can be costly and time consuming to obtain failure time data under normal operating conditions if the mean time to failure of a product is long. An alternative is to use failure time data from an accelerated life test (ALT) to extrapolate the reliability under normal conditions. In accelerated life testing, the units are placed under a higher than normal stress condition such as voltage, current, pressure, temperature, to make the items fail in a shorter period of time. The failure information is then transformed through an accelerated model commonly known as the time transformation function, to predict the reliability under normal operating conditions. The power law will be used as the time transformation function in this thesis. We will first consider a Bayesian inference model under the assumption that the underlying life distribution in the accelerated life test is exponentially distributed. The maximal data information (MDI) prior, the Ghosh Mergel and Liu (GML) prior and the Jeffreys prior will be derived for the exponential distribution. The propriety of the posterior distributions will be investigated. Results will be compared when using these non-informative priors in a simulation study by looking at the posterior variances. The Weibull distribution as the underlying life distribution in the accelerated life test will also be investigated. The maximal data information prior will be derived for the Weibull distribution using the power law. The uniform prior and a mixture of Gamma and uniform priors will be considered. The propriety of these posteriors will also be investigated. The predictive reliability at the use-stress will be computed for these models. The deviance information criterion will be used to compare these priors. As a result of using a time transformation function, Bayesian inference becomes analytically intractable and Markov Chain Monte Carlo (MCMC) methods will be used to alleviate this problem. The Metropolis-Hastings algorithm will be used to sample from the posteriors for the exponential model in the accelerated life test. The adaptive rejection sampling method will be used to sample from the posterior distributions when the Weibull model is considered.
- Full Text:
- Authors: Izally, Sharkay Ruwade
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3003 , vital:20351
- Description: Reliability life testing is used for life data analysis in which samples are tested under normal conditions to obtain failure time data for reliability assessment. It can be costly and time consuming to obtain failure time data under normal operating conditions if the mean time to failure of a product is long. An alternative is to use failure time data from an accelerated life test (ALT) to extrapolate the reliability under normal conditions. In accelerated life testing, the units are placed under a higher than normal stress condition such as voltage, current, pressure, temperature, to make the items fail in a shorter period of time. The failure information is then transformed through an accelerated model commonly known as the time transformation function, to predict the reliability under normal operating conditions. The power law will be used as the time transformation function in this thesis. We will first consider a Bayesian inference model under the assumption that the underlying life distribution in the accelerated life test is exponentially distributed. The maximal data information (MDI) prior, the Ghosh Mergel and Liu (GML) prior and the Jeffreys prior will be derived for the exponential distribution. The propriety of the posterior distributions will be investigated. Results will be compared when using these non-informative priors in a simulation study by looking at the posterior variances. The Weibull distribution as the underlying life distribution in the accelerated life test will also be investigated. The maximal data information prior will be derived for the Weibull distribution using the power law. The uniform prior and a mixture of Gamma and uniform priors will be considered. The propriety of these posteriors will also be investigated. The predictive reliability at the use-stress will be computed for these models. The deviance information criterion will be used to compare these priors. As a result of using a time transformation function, Bayesian inference becomes analytically intractable and Markov Chain Monte Carlo (MCMC) methods will be used to alleviate this problem. The Metropolis-Hastings algorithm will be used to sample from the posteriors for the exponential model in the accelerated life test. The adaptive rejection sampling method will be used to sample from the posterior distributions when the Weibull model is considered.
- Full Text:
Being civil
- Matshoba, Zongezile Theophilus
- Authors: Matshoba, Zongezile Theophilus
- Date: 2016
- Language: English , Xhosa
- Type: Thesis , Masters , MA
- Identifier: vital:6017 , http://hdl.handle.net/10962/d1021237
- Description: My collection of short stories delves into government and governance, democracy, citizenship, civil servants, poverty, corruption and nepotism. My stories draw on the traditions of gritty urban crime fiction uncovering crimes of violence, service delivery, vandalism and corruption. They explore themes of mental cruelty and greed, self- preservation and community in rural areas, farms, townships and cities characterized by wrenching contradictions and inequalities. , This epic dramatic poetic verse delves into government and school governance, labour unions, liberation struggle, parenting and a wide range of school perceptions. It interrogates the roles of parents, teachers, students, department of education officials and that of other stakeholders that make use of schools. Influenced by William Wellington Gqoba’s ‘A great debate on education: a Parable’ wayback, it continues the education debate in the current post-democratic South Africa characterized by wrenching contradictions and inequalities. , This thesis is presented in two parts: English and isiXhosa.
- Full Text:
- Authors: Matshoba, Zongezile Theophilus
- Date: 2016
- Language: English , Xhosa
- Type: Thesis , Masters , MA
- Identifier: vital:6017 , http://hdl.handle.net/10962/d1021237
- Description: My collection of short stories delves into government and governance, democracy, citizenship, civil servants, poverty, corruption and nepotism. My stories draw on the traditions of gritty urban crime fiction uncovering crimes of violence, service delivery, vandalism and corruption. They explore themes of mental cruelty and greed, self- preservation and community in rural areas, farms, townships and cities characterized by wrenching contradictions and inequalities. , This epic dramatic poetic verse delves into government and school governance, labour unions, liberation struggle, parenting and a wide range of school perceptions. It interrogates the roles of parents, teachers, students, department of education officials and that of other stakeholders that make use of schools. Influenced by William Wellington Gqoba’s ‘A great debate on education: a Parable’ wayback, it continues the education debate in the current post-democratic South Africa characterized by wrenching contradictions and inequalities. , This thesis is presented in two parts: English and isiXhosa.
- Full Text:
Being for the Other: Surveillance and Depictions of Race, Gender, and Animals in Contemporary South African Fiction
- Authors: Laue, Kharys Ateh
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/3848 , vital:20549
- Description: This thesis examines the depiction, in contemporary South African fiction, of irresponsibility and responsibility in relation to the raced, gendered, and animal Other. Through a close analysis of Jeremy Bentham’s Panopticon prison and Michel Foucault’s study of this design, I establish the notion of disciplinary surveillance or panopticism. This I take to be a mode of power that seeks, by means of an invisible gaze, to render its subjects docile. In my readings of J. M. Coetzee’s Waiting for the Barbarians, Zoë Wicomb’s Playing in the Light, Justin Cartwright’s White Lightning, and selected short stories from Wicomb’s You Can’t Get Lost in Cape Town and The One That Got Away, I demonstrate that oppressive authoritarian regimes are rooted in Benthamic principles of hyper-visibility and concealment. Disciplinary power, I contend, is effective precisely because it places an individual in a constant state of Being-for-Others, a term coined by Jean-Paul Sartre to describe the experience of objectification through another’s look. Judith Butler’s concept of gender performativity and W. E. B. Du Bois’s notion of black double consciousness frame my examination of, respectively, gender and racial oppression, while my discussion of animals appeals to Jacques Derrida’s work on the non-human. I show how surveillance, in each of the selected texts, functions through a racist and/or sexist and/or speciesist gaze that facilitates violent, irresponsible relationships with the human and non-human Other. The texts under discussion, however, also depict ways in which the Other actively resists and subverts regimes of oppression, often by means of a counter gaze that compels the protagonist, or the reader, to take up responsibility for Others. Ultimately, my study concludes that the fictional works of Coetzee, Wicomb, and Cartwright offer an ethics of empathetic responsibility, which I term Being for the Other, in opposition to mechanisms of disciplinary surveillance that seek to oppress, conceal, and dominate.
- Full Text:
- Authors: Laue, Kharys Ateh
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/3848 , vital:20549
- Description: This thesis examines the depiction, in contemporary South African fiction, of irresponsibility and responsibility in relation to the raced, gendered, and animal Other. Through a close analysis of Jeremy Bentham’s Panopticon prison and Michel Foucault’s study of this design, I establish the notion of disciplinary surveillance or panopticism. This I take to be a mode of power that seeks, by means of an invisible gaze, to render its subjects docile. In my readings of J. M. Coetzee’s Waiting for the Barbarians, Zoë Wicomb’s Playing in the Light, Justin Cartwright’s White Lightning, and selected short stories from Wicomb’s You Can’t Get Lost in Cape Town and The One That Got Away, I demonstrate that oppressive authoritarian regimes are rooted in Benthamic principles of hyper-visibility and concealment. Disciplinary power, I contend, is effective precisely because it places an individual in a constant state of Being-for-Others, a term coined by Jean-Paul Sartre to describe the experience of objectification through another’s look. Judith Butler’s concept of gender performativity and W. E. B. Du Bois’s notion of black double consciousness frame my examination of, respectively, gender and racial oppression, while my discussion of animals appeals to Jacques Derrida’s work on the non-human. I show how surveillance, in each of the selected texts, functions through a racist and/or sexist and/or speciesist gaze that facilitates violent, irresponsible relationships with the human and non-human Other. The texts under discussion, however, also depict ways in which the Other actively resists and subverts regimes of oppression, often by means of a counter gaze that compels the protagonist, or the reader, to take up responsibility for Others. Ultimately, my study concludes that the fictional works of Coetzee, Wicomb, and Cartwright offer an ethics of empathetic responsibility, which I term Being for the Other, in opposition to mechanisms of disciplinary surveillance that seek to oppress, conceal, and dominate.
- Full Text:
Beta decay of 100/400 Zr produced in neutron-induced fission of natural uranium
- Authors: Kamoto, Thokozani
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3024 , vital:20353
- Description: Fission fragments, produced by neutron bombardment of natural uranium at the Physics Department, Jyväskylä, Finland, are studied in this work. The data had been sorted into 25 Y — y coincidence matrices which were then analysed. In this work we aimed to identify the fission products using Y-Y coincidence analysis and then study the beta-decay of some of the fission products. Sixteen fission products ranging from A = 94 to A = 136 were identified. Out of these fission products beta decay of the A = 100 (100/40 Zr – 100/41 Nb – 100/42 Mo) chain was studied in greater detail. We have also studied the variation of the relative intensities as a function of time of the 159-, 528-, 600-, 768-, 928- and 1502-keV Y-rav lines in 100/42 Mo and the profiles of the relative intensities have been modelled with the variation of the activity of 100/41 Nb against time. Configuration assignments of 100 Zr and 100/42 Mo are discussed.
- Full Text:
- Authors: Kamoto, Thokozani
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3024 , vital:20353
- Description: Fission fragments, produced by neutron bombardment of natural uranium at the Physics Department, Jyväskylä, Finland, are studied in this work. The data had been sorted into 25 Y — y coincidence matrices which were then analysed. In this work we aimed to identify the fission products using Y-Y coincidence analysis and then study the beta-decay of some of the fission products. Sixteen fission products ranging from A = 94 to A = 136 were identified. Out of these fission products beta decay of the A = 100 (100/40 Zr – 100/41 Nb – 100/42 Mo) chain was studied in greater detail. We have also studied the variation of the relative intensities as a function of time of the 159-, 528-, 600-, 768-, 928- and 1502-keV Y-rav lines in 100/42 Mo and the profiles of the relative intensities have been modelled with the variation of the activity of 100/41 Nb against time. Configuration assignments of 100 Zr and 100/42 Mo are discussed.
- Full Text:
Between sentences
- Authors: Thesen, Jo-Ann
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6007 , http://hdl.handle.net/10962/1021215
- Description: My stories explore different forms, including flash fiction. Some use the fairy tale form to combine fiction and non-fiction in order to reach the essence of the story. In this I am influenced by Kate Bernheimer, who speaks of the “flatness, abstraction, intuitive logic and normalized magic” of traditional fairy tales. A number of stories are set in the places I worked as a newspaper reporter. Here I use my old press reports as starting points for the real or imagined story behind the news – often involving miscommunication, dominance, exploitation, the tension between isolation and belonging, and the nuances of family relationships.
- Full Text:
- Authors: Thesen, Jo-Ann
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6007 , http://hdl.handle.net/10962/1021215
- Description: My stories explore different forms, including flash fiction. Some use the fairy tale form to combine fiction and non-fiction in order to reach the essence of the story. In this I am influenced by Kate Bernheimer, who speaks of the “flatness, abstraction, intuitive logic and normalized magic” of traditional fairy tales. A number of stories are set in the places I worked as a newspaper reporter. Here I use my old press reports as starting points for the real or imagined story behind the news – often involving miscommunication, dominance, exploitation, the tension between isolation and belonging, and the nuances of family relationships.
- Full Text:
Bio-prospecting a Soil Metagenomic Library for Carbohydrate Active Esterases
- Authors: Shezi, Ntombifuthi
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4172 , http://hdl.handle.net/10962/d1021266
- Description: Lignocellulosic biomass is a promising renewable resource on earth. Plant biomass contains fermentable sugars and other moieties that can be converted to biofuels or other chemicals. Enzymatic hydrolysis of these biopolymers is significant in the liberation of sugars for fermentation into desired products. Owing to its complex structure, synergistic action of enzymes is required for its degradation. Enzymes that are involved in biomass degradation include cellulases, hemicellulases and the accessory enzymes acetyl xylan esterases and ferulic acid esterases. Ferulic acid esterases (FAEs, EC 3.1.1.73), represent a subclass of carboxylester hydrolases (EC 3.1.1.-) that catalyse the release of hydroxycinnamic acids (such as ferulic acid, p-coumaric, ferulic, sinapic and caffeic acid) that are generally found esterified to polysaccharides, such as arabinoxylans. Hydroxycinnamic acids have widespread potential applications due to their antimicrobial, photoprotectant and antioxidant properties, as well as their use as flavour precursors. Therefore, this interesting group of FAEs has a potentially wide variety of applications in agriculture, food and pharmaceutical industries. In the search for novel biocatalysts, metagenomics is considered as an alternative approach to conventional microbe screening, therefore, searching for novel biocatalysts from a soil metagenome that harbours a unique diversity of biocatalyst is significant. The aim of this study was to extract DNA from soil associated with cattle manure and construct a soil metagenomic library using a fosmid based plasmid vector and subsequently functionally screen for ferulic acid esterases using ethyl ferulate as a model substrate. A total of 59 recombinant fosmids conferring ferulic acid esterase phenotypes were identified (Hit rate 1:3122) and the two fosmids that consistently showed high FAE activities were selected for further study. Following nucleotide sequencing and translational analysis, two fae encoding open reading frames (FAE9 and FAE27) of approximately 274 and 322 aa, respectively, were identified. The amino acid sequence of the two ORFs contained a classical conserved esterase/lipase G-x-S-x-G sequence motif. The two genes (fae9 and fae27) were successfully expressed in Escherichia coli BL21 (DE3) and the purified enzymes exhibited respective temperature optima of 50 °C and 40 °C, and respective pH optima of 6.0 and 7.0. Further biochemical characterisation showed that FAE9 and FAE27 have high substrate specificity, following the fact that EFA is the preferred substrate for FAE9 (kcat/Km value of 128 s−1.mM-1) and also the preferred substrate for FAE27 (kcat/Km value of 137 s−1.mM-1). This work proves that soil is a valuable environmental source for novel esterase screening through functional based metagenomic approach. Therefore, this method may be used to screen for other valuable enzymes from environmental sources using inexpensive natural sources to encourage the screening of specific enzymes. Biochemistry of the two isolated enzymes makes these enzymes to be useful in industrial applications due to broad substrate activity that could replace the specialised enzymes to complete plant biomass degradation.
- Full Text:
- Authors: Shezi, Ntombifuthi
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4172 , http://hdl.handle.net/10962/d1021266
- Description: Lignocellulosic biomass is a promising renewable resource on earth. Plant biomass contains fermentable sugars and other moieties that can be converted to biofuels or other chemicals. Enzymatic hydrolysis of these biopolymers is significant in the liberation of sugars for fermentation into desired products. Owing to its complex structure, synergistic action of enzymes is required for its degradation. Enzymes that are involved in biomass degradation include cellulases, hemicellulases and the accessory enzymes acetyl xylan esterases and ferulic acid esterases. Ferulic acid esterases (FAEs, EC 3.1.1.73), represent a subclass of carboxylester hydrolases (EC 3.1.1.-) that catalyse the release of hydroxycinnamic acids (such as ferulic acid, p-coumaric, ferulic, sinapic and caffeic acid) that are generally found esterified to polysaccharides, such as arabinoxylans. Hydroxycinnamic acids have widespread potential applications due to their antimicrobial, photoprotectant and antioxidant properties, as well as their use as flavour precursors. Therefore, this interesting group of FAEs has a potentially wide variety of applications in agriculture, food and pharmaceutical industries. In the search for novel biocatalysts, metagenomics is considered as an alternative approach to conventional microbe screening, therefore, searching for novel biocatalysts from a soil metagenome that harbours a unique diversity of biocatalyst is significant. The aim of this study was to extract DNA from soil associated with cattle manure and construct a soil metagenomic library using a fosmid based plasmid vector and subsequently functionally screen for ferulic acid esterases using ethyl ferulate as a model substrate. A total of 59 recombinant fosmids conferring ferulic acid esterase phenotypes were identified (Hit rate 1:3122) and the two fosmids that consistently showed high FAE activities were selected for further study. Following nucleotide sequencing and translational analysis, two fae encoding open reading frames (FAE9 and FAE27) of approximately 274 and 322 aa, respectively, were identified. The amino acid sequence of the two ORFs contained a classical conserved esterase/lipase G-x-S-x-G sequence motif. The two genes (fae9 and fae27) were successfully expressed in Escherichia coli BL21 (DE3) and the purified enzymes exhibited respective temperature optima of 50 °C and 40 °C, and respective pH optima of 6.0 and 7.0. Further biochemical characterisation showed that FAE9 and FAE27 have high substrate specificity, following the fact that EFA is the preferred substrate for FAE9 (kcat/Km value of 128 s−1.mM-1) and also the preferred substrate for FAE27 (kcat/Km value of 137 s−1.mM-1). This work proves that soil is a valuable environmental source for novel esterase screening through functional based metagenomic approach. Therefore, this method may be used to screen for other valuable enzymes from environmental sources using inexpensive natural sources to encourage the screening of specific enzymes. Biochemistry of the two isolated enzymes makes these enzymes to be useful in industrial applications due to broad substrate activity that could replace the specialised enzymes to complete plant biomass degradation.
- Full Text:
Biofuels and rural development: A case study of the Mapfura-Makhura Incubator and small-scale farmers in the Limpopo Province
- Authors: Mothupi, Frans Makwena
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/1488 , vital:20062
- Description: The primary objective of the study is to examine the livelihood impact of biofuel production on small-scale rural farmers participating in the MMI project in the local districts in Limpopo province. The study is theoretically underpinned by the sustainable livelihoods framework which provides a nuanced analysis of the complex nature of poverty and livelihoods. The framework was used to examine how MMI as a structure with its own processes (incubator model) plays a role in creating a means for farmers to have more access to livelihood assets which would help them achieve improved livelihoods outcome. The findings of this study reflect what has already been articulated in literature about small-scale biofuel projects. This study shows that MMI’s incubator plays an important role for 73% of the farmers to access all of the livelihood assets and ultimately improving their farm income and food security. Furthermore, 90% of the participants admitted to have received support from MMI in a number of ways. This includes the provision of farming inputs, labour and access to markets in addition to training, mentoring and coaching. The study also found that despite the overwhelming support, both MMI and farmers face a number of challenges. Farmers still lack adequate farming inputs, transport, access to markets and vulnerability to natural disasters. MMI faces challenges in raising funds to provide inputs to all their incubatees and lack of adequate mechanization. Lack of transportation affects both farmers and MMI in that farmers find it difficult to access MMI service. In the same light, MMI has found it difficult to reach farmers for post-incubation, coaching and mentoring or delivering inputs; this can be challenging and a costly process. MMI’s biofuel production project has the capability for improving rural livelihoods through agriculture. The study concludes by recommending that MMI should improve its own capacity in order for them to better the lives of the farmers they assist. This study is significant for contributing to a field which has received less academic and research attention in South Africa. Its scholarly contribution will enhance the existing body of knowledge on biofuels and rural development in South Africa
- Full Text:
- Authors: Mothupi, Frans Makwena
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/1488 , vital:20062
- Description: The primary objective of the study is to examine the livelihood impact of biofuel production on small-scale rural farmers participating in the MMI project in the local districts in Limpopo province. The study is theoretically underpinned by the sustainable livelihoods framework which provides a nuanced analysis of the complex nature of poverty and livelihoods. The framework was used to examine how MMI as a structure with its own processes (incubator model) plays a role in creating a means for farmers to have more access to livelihood assets which would help them achieve improved livelihoods outcome. The findings of this study reflect what has already been articulated in literature about small-scale biofuel projects. This study shows that MMI’s incubator plays an important role for 73% of the farmers to access all of the livelihood assets and ultimately improving their farm income and food security. Furthermore, 90% of the participants admitted to have received support from MMI in a number of ways. This includes the provision of farming inputs, labour and access to markets in addition to training, mentoring and coaching. The study also found that despite the overwhelming support, both MMI and farmers face a number of challenges. Farmers still lack adequate farming inputs, transport, access to markets and vulnerability to natural disasters. MMI faces challenges in raising funds to provide inputs to all their incubatees and lack of adequate mechanization. Lack of transportation affects both farmers and MMI in that farmers find it difficult to access MMI service. In the same light, MMI has found it difficult to reach farmers for post-incubation, coaching and mentoring or delivering inputs; this can be challenging and a costly process. MMI’s biofuel production project has the capability for improving rural livelihoods through agriculture. The study concludes by recommending that MMI should improve its own capacity in order for them to better the lives of the farmers they assist. This study is significant for contributing to a field which has received less academic and research attention in South Africa. Its scholarly contribution will enhance the existing body of knowledge on biofuels and rural development in South Africa
- Full Text:
Biology and ecology of Glossogobius callidus (Smith 1937) in irrigation impoundments in the Sundays River Valley of the Eastern Cape
- Authors: Mofu, Lubabalo
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/1136 , vital:20023
- Description: The River Goby Glossogobius callidus (Smith, 1937) is a native abundant fish in both freshwater and estuarine habitats in the Cape Fold Ecoregion, yet little information is available on its life-history. This study aims to contribute to knowledge on the age and growth, reproductive biology and the diet and feeding habits of G. callidus in irrigation impoundments. Glossogobius callidus was sampled monthly from August 2013 till March 2015; from the irrigation ponds in the Sundays River Valley, Eastern Cape Province, South Africa. To determine sex, gonads were visually assessed under a dissecting microscope to confirm the sex based on the shape of the urogenital papillae. Fish were then dissected to confirm sex and gonads were categorised into five macroscopic stages which were histologically validated. Microscopic stages of gonadal development were discerned based on nuclear and cytoplasmic characteristics of the oocyte or sperm. Ovaries and sperms were assigned stages based on the most advanced type of oocyte present. In total 2054 fishes ranging in length from 21.1 mm to 137.2 mm TL were sampled. The sex ratio (1.1 males: 1 females) did not differ from unity (x2 = 0.027, df = 1, p = 0.87). Length at 50% maturity (Lm) was 70 mm TL females and 72 mm TL for males. Spawning season was mid-spring and mid-summer and mean ± S.D absolute fecundity was estimated at 1028.2 ± 131.7 ova/fish. Relative fecundity (number of vitellogenic oocytes per gram of eviscerated fish mass) were estimated at 50 ± 18 ova/fish gram. Otoliths from 560 fish were used for ageing. Growth zone deposition rate was validated using edge analysis. As a unimodal periodic regression model best described the temporal proportion of opaque zone deposition on the edge of otoliths over a one-year period, growth zone deposition rate was validated as annual. The oldest female fish was a 4-year old 84.4 mm TL fish and the oldest male was a 7-year old 100.5 mm TL fish. The length-at-age for the entire population of 560 G. callidus provided von Bertalanffy parameters of Lt = 92 (1 - e -0.58(t + 0.4)) mm TL for the entire population, Lt = 70 (1 - e -1.8 (t + 0.06)) mm TL for males and Lt = 65 (1 - e -1.8 (t + 0.05)) mm TL for females. Converting length at maturity to age at maturity demonstrated that G. callidus attained maturity at an age of 2-years. Growth performance described using the phi-prime index showed that G. callidus had lower growth performance compared to the invasive Neogobius melanostomus. Using age structure, natural mortality was estimated at 1.31 yr-1 using catch curve analysis. Diet of G. callidus comprised of ten taxonomic groups. Among these, aquatic invertebrates were the most diverse group but while relative contribution of the dietary components varied across all size classes and seasons, the key prey items were consistently found in all size classes. These were Diptera, Hemiptera, Trichoptera, Odonata, Cladocera, Copepoda, Hydracarina, Amphipoda, Crustacea, and Mollusca. While dietary differences were observed between the size classes and throughout the seasons, G. callidus can be regarded as a generalist feeder preying on an array of different species. Given its abundance and diet, I suggest that G. callidus contribute considerably to the invertebrate predation pressure in these artificial aquatic environments in an arid region. In summary, medium fecundity, fast growth, moderate maturity, and a generalist feeding behaviour demonstrate that G. callidus is an equilibrium life strategist. In comparison with other species, the life-history traits of G. callidus from irrigation impoundments resemble those of other freshwater goby species, some of which are global invaders.
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- Authors: Mofu, Lubabalo
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/1136 , vital:20023
- Description: The River Goby Glossogobius callidus (Smith, 1937) is a native abundant fish in both freshwater and estuarine habitats in the Cape Fold Ecoregion, yet little information is available on its life-history. This study aims to contribute to knowledge on the age and growth, reproductive biology and the diet and feeding habits of G. callidus in irrigation impoundments. Glossogobius callidus was sampled monthly from August 2013 till March 2015; from the irrigation ponds in the Sundays River Valley, Eastern Cape Province, South Africa. To determine sex, gonads were visually assessed under a dissecting microscope to confirm the sex based on the shape of the urogenital papillae. Fish were then dissected to confirm sex and gonads were categorised into five macroscopic stages which were histologically validated. Microscopic stages of gonadal development were discerned based on nuclear and cytoplasmic characteristics of the oocyte or sperm. Ovaries and sperms were assigned stages based on the most advanced type of oocyte present. In total 2054 fishes ranging in length from 21.1 mm to 137.2 mm TL were sampled. The sex ratio (1.1 males: 1 females) did not differ from unity (x2 = 0.027, df = 1, p = 0.87). Length at 50% maturity (Lm) was 70 mm TL females and 72 mm TL for males. Spawning season was mid-spring and mid-summer and mean ± S.D absolute fecundity was estimated at 1028.2 ± 131.7 ova/fish. Relative fecundity (number of vitellogenic oocytes per gram of eviscerated fish mass) were estimated at 50 ± 18 ova/fish gram. Otoliths from 560 fish were used for ageing. Growth zone deposition rate was validated using edge analysis. As a unimodal periodic regression model best described the temporal proportion of opaque zone deposition on the edge of otoliths over a one-year period, growth zone deposition rate was validated as annual. The oldest female fish was a 4-year old 84.4 mm TL fish and the oldest male was a 7-year old 100.5 mm TL fish. The length-at-age for the entire population of 560 G. callidus provided von Bertalanffy parameters of Lt = 92 (1 - e -0.58(t + 0.4)) mm TL for the entire population, Lt = 70 (1 - e -1.8 (t + 0.06)) mm TL for males and Lt = 65 (1 - e -1.8 (t + 0.05)) mm TL for females. Converting length at maturity to age at maturity demonstrated that G. callidus attained maturity at an age of 2-years. Growth performance described using the phi-prime index showed that G. callidus had lower growth performance compared to the invasive Neogobius melanostomus. Using age structure, natural mortality was estimated at 1.31 yr-1 using catch curve analysis. Diet of G. callidus comprised of ten taxonomic groups. Among these, aquatic invertebrates were the most diverse group but while relative contribution of the dietary components varied across all size classes and seasons, the key prey items were consistently found in all size classes. These were Diptera, Hemiptera, Trichoptera, Odonata, Cladocera, Copepoda, Hydracarina, Amphipoda, Crustacea, and Mollusca. While dietary differences were observed between the size classes and throughout the seasons, G. callidus can be regarded as a generalist feeder preying on an array of different species. Given its abundance and diet, I suggest that G. callidus contribute considerably to the invertebrate predation pressure in these artificial aquatic environments in an arid region. In summary, medium fecundity, fast growth, moderate maturity, and a generalist feeding behaviour demonstrate that G. callidus is an equilibrium life strategist. In comparison with other species, the life-history traits of G. callidus from irrigation impoundments resemble those of other freshwater goby species, some of which are global invaders.
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Boetie is verlore: the reproduction of militarized white masculinities through the lens of Boetie gaan border toe! (1984)
- Authors: Coetzee, Joseph
- Date: 2016
- Language: English
- Type: Thesis , Masters , MFA
- Identifier: http://hdl.handle.net/10962/4245 , vital:20638
- Description: My father fought for the South African Defence Force (SADF) from 1983 to 1985. At that time the apartheid regime was involved in extensive military operations in what is now Namibia and Angola. This conflict was aimed at quelling the liberation movements in those countries and, as Gary Baines has noted, supported the United States of America’s Cold War interests (Baines 2007:1). When I was fourteen I found a piece written by my father in which he remembers the first person that he had killed in the aforementioned conflict. This was a child soldier who he compared to me as I was a similar age at the time of his writing. The idea of my father as a killer haunted me. He has carried the trauma of his experiences on the border with him; he has told me how the dead visit him in dreams. On the one hand, these memories, not my own, have been constructed through my interpretation of the events in my father’s stories. On the other hand, homologies may be drawn between his actual experiences and a fantasy representation of the conflict I have encountered, the film Boetie gaan border toe! or Brother goes to the border! (1984). This apartheid propaganda film presents an idealised representation of the conflict from the point of view of the apartheid state. The protagonist of the film, Boetie, is an example of the aspirational and dominant image of militarised masculinity the apartheid state wished young white men to emulate. The racist sexist, patriarchal and materialistic reality created within the film is one I am familiar with. The toys I grew up playing with, television shows, films, advertising and popular culture I consumed, alongside the boys’ school I attended and the University I currently attend are all rooted in and continue to reproduce this reality. I have encountered many similar archetypes to the Boetie character. With this in mind I wish through my art practice to create a work which draws upon my father’s writing and imagery from Boetie gaan border toe! (1984). I have placed these alongside windows into my contemporary context in order to emphasise the continual reproduction of these ideas. In reference to the Boetie film I have decided to create my own film entitled Boetie is verlore or Brother is lost. This is a magic realist documentary film that I have constructed through various interviews and fantasy dream sequences in order to paint a picture of the continual incubation and reproduction of realities similar to that of Boetie. Boetie is a rich white man who is characterised through his material possessions and his compulsive heterosexuality. White women are interchangeable to him whilst blackness in the film is made completely invisible. In South Africa such representations are strongly linked to the question of land and naturalising the white male coloniser’s dominance and privilege.
- Full Text:
- Authors: Coetzee, Joseph
- Date: 2016
- Language: English
- Type: Thesis , Masters , MFA
- Identifier: http://hdl.handle.net/10962/4245 , vital:20638
- Description: My father fought for the South African Defence Force (SADF) from 1983 to 1985. At that time the apartheid regime was involved in extensive military operations in what is now Namibia and Angola. This conflict was aimed at quelling the liberation movements in those countries and, as Gary Baines has noted, supported the United States of America’s Cold War interests (Baines 2007:1). When I was fourteen I found a piece written by my father in which he remembers the first person that he had killed in the aforementioned conflict. This was a child soldier who he compared to me as I was a similar age at the time of his writing. The idea of my father as a killer haunted me. He has carried the trauma of his experiences on the border with him; he has told me how the dead visit him in dreams. On the one hand, these memories, not my own, have been constructed through my interpretation of the events in my father’s stories. On the other hand, homologies may be drawn between his actual experiences and a fantasy representation of the conflict I have encountered, the film Boetie gaan border toe! or Brother goes to the border! (1984). This apartheid propaganda film presents an idealised representation of the conflict from the point of view of the apartheid state. The protagonist of the film, Boetie, is an example of the aspirational and dominant image of militarised masculinity the apartheid state wished young white men to emulate. The racist sexist, patriarchal and materialistic reality created within the film is one I am familiar with. The toys I grew up playing with, television shows, films, advertising and popular culture I consumed, alongside the boys’ school I attended and the University I currently attend are all rooted in and continue to reproduce this reality. I have encountered many similar archetypes to the Boetie character. With this in mind I wish through my art practice to create a work which draws upon my father’s writing and imagery from Boetie gaan border toe! (1984). I have placed these alongside windows into my contemporary context in order to emphasise the continual reproduction of these ideas. In reference to the Boetie film I have decided to create my own film entitled Boetie is verlore or Brother is lost. This is a magic realist documentary film that I have constructed through various interviews and fantasy dream sequences in order to paint a picture of the continual incubation and reproduction of realities similar to that of Boetie. Boetie is a rich white man who is characterised through his material possessions and his compulsive heterosexuality. White women are interchangeable to him whilst blackness in the film is made completely invisible. In South Africa such representations are strongly linked to the question of land and naturalising the white male coloniser’s dominance and privilege.
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bones & flesh
- Authors: Hammerton, Kerry
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6009 , http://hdl.handle.net/10962/d1021221
- Description: My collection encompasses personal relationships, intimacy, and the erotic, as well as more narrative poems grounded in landscapes, including urban and internal landscapes. Some were written in conversation with other poems or pieces of prose such as the stories of Noy Holland. I use free verse forms influenced by various prose poems as well as by the musical/tonal forms of poets such as Lorca. Other styles and influences include the darkness and directness of Spanish poetry particularly Rafael Alberti (esp. his book Concerning the Angels); and the confident and reflective style of Romanian poet Nina Cassian.
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- Authors: Hammerton, Kerry
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6009 , http://hdl.handle.net/10962/d1021221
- Description: My collection encompasses personal relationships, intimacy, and the erotic, as well as more narrative poems grounded in landscapes, including urban and internal landscapes. Some were written in conversation with other poems or pieces of prose such as the stories of Noy Holland. I use free verse forms influenced by various prose poems as well as by the musical/tonal forms of poets such as Lorca. Other styles and influences include the darkness and directness of Spanish poetry particularly Rafael Alberti (esp. his book Concerning the Angels); and the confident and reflective style of Romanian poet Nina Cassian.
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