Post release evaluation of the distribution and efficacy of Eccritotarsus catarinensis and Eccritotarsus eichhorniae on Pontederia crassipes in South Africa
- Authors: Maseko, Zolile
- Date: 2020
- Subjects: Water hyacinth -- Biological control -- South Africa , Weeds -- Biological control -- South Africa , Miridae -- South Africa , Insects as biological pest control agents -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/143046 , vital:38196
- Description: Biological control involves the release of new species into the environment and therefore, needs to be carefully monitored through post-release assessments which have been largely neglected in the science. Post-release evaluations of biological control programmes reveal whether the control agent has established and if it impacts weed demography, while cost-benefit analyses require a different set of data that show the magnitude on return on investment. The biological control effort on Pontederia crassipes in South Africa uses, amongst others, two species of mirid, Eccritotarsus catarinensis and E. eichhorniae. Initially, they were released as a single species, but were recently divided using molecular techniques. Eccritotarsus catarinensis was released in 1999, and E. eichhorniae in 2007. After many releases over two decades, there was need to assess where each species was established in the country. Molecular techniques proved to be valuable in identifying the two species as they are morphologically indistinguishable in the field. Therefore, molecular techniques should be routinely used for screening biocontrol agents, whether new or as re-introductions. Annual surveys of the mirid release sites around South Africa were undertaken between 2016 and 2019. At each site both insect and plant parameters were measured. Only E. catarinensis is established in the field in South Africa despite the multiple releases of E. eichhorniae at over 70 sites across the country, and E. catarinensis has established at only 22 of the 45 release sites accessed during this study. This thesis tested climate, interaction with other agents already on P. crassipes, and direct competition between the two mirid species as reasons for the lack of establishment of E. eichhorniae. The results of the country-wide surveys showed that climate and water trophic status were the major determinants in the establishment of E. catarinensis. Most of the establishment was recorded in the warmer regions of the country, however, a few populations of the mirid also established in cooler areas, thus demonstrating a degree of thermal plasticity, and possible microclimates as the mirids persisted at sites shaded by riparian vegetation. Stochastic events such as active herbicide campaigns, winter frosts, droughts and floods were responsible for the absence of the mirid at some sites. At some of the eutrophic sites, despite the abundance of E. catarinensis, plants still proliferated as the water trophic status facilitated plant growth, thus, plants were able to compensate for the damage inflicted by the mirid. A more intensive, monthly, post-release evaluation was conducted on the Kubusi River, Eastern Cape Province between 2016 and 2019. This is regarded as one of the cooler water hyacinth sites. Populations of biological control agents at this site fluctuated seasonally. At this site, cold winters caused frosting of the leaves of P. crassipes with the exception of plants growing under overhanging vegetation that provided a refuge for the mirid. But, cool temperatures in the winter months (May to August) severely reduced the populations of E. catarinensis that required a long recovery phase in spring. The consequence of this was that the plants grew unchecked from the onset of the growing season forming dense mats. Of the four agents at the Kubusi River site, Eccritotarsus catarinensis recovered slowest after winter, with lag phases ranging from two months to several months of the three-year period. The release of a suite of agents has implications on the agents themselves, where interactions between the agents can be important. Interactions between pairs and even multiple agents can have implications for biocontrol, where agents are either complimentary or interfere with each other. In this case, because E. catarinensis recovered the slowest of the four agents at the site, plants were of a poor quality by mid-summer resulting in low mirid populations. Competition in weed biological control could be expected to be strongest between pairs of agents that share the same niche, and this could be the reason why E. eichhorniae failed to establish at sites where E. catarinensis had already been established for several years. When the two mirids were combined in manipulated trials in a polytunnel, populations were lower compared to when the two mirids occurred separately. Under warm conditions, it is likely that E. eichhorniae would be the superior agent compared to E. catarinensis. The evaluations discussed in this thesis highlighted gaps in agent release methodology in multispecies settings, as well as the need for strategic augmentation pre- and post-winter. It is important to release agents that will complement each other rather than compete, therefore, when releasing agents in a multispecies setting, niche differentiation needs to be considered. Here it is concluded that the best practice for dealing with the mirids is that they should be released individually, and at sites that have no other biological control agents in order to ultimately assess their efficacy. Landscape level, long-term monitoring of biological control programmes shows the impact of the control programme at a broader scale and, are far more informative than short-term studies and at fewer sites. Long-term post-release evaluations should be mandatory in biological control programmes. Furthermore, these assessments will help develop new strategies or improve on existing ones, thus achieve greater success in control.
- Full Text:
- Date Issued: 2020
- Authors: Maseko, Zolile
- Date: 2020
- Subjects: Water hyacinth -- Biological control -- South Africa , Weeds -- Biological control -- South Africa , Miridae -- South Africa , Insects as biological pest control agents -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/143046 , vital:38196
- Description: Biological control involves the release of new species into the environment and therefore, needs to be carefully monitored through post-release assessments which have been largely neglected in the science. Post-release evaluations of biological control programmes reveal whether the control agent has established and if it impacts weed demography, while cost-benefit analyses require a different set of data that show the magnitude on return on investment. The biological control effort on Pontederia crassipes in South Africa uses, amongst others, two species of mirid, Eccritotarsus catarinensis and E. eichhorniae. Initially, they were released as a single species, but were recently divided using molecular techniques. Eccritotarsus catarinensis was released in 1999, and E. eichhorniae in 2007. After many releases over two decades, there was need to assess where each species was established in the country. Molecular techniques proved to be valuable in identifying the two species as they are morphologically indistinguishable in the field. Therefore, molecular techniques should be routinely used for screening biocontrol agents, whether new or as re-introductions. Annual surveys of the mirid release sites around South Africa were undertaken between 2016 and 2019. At each site both insect and plant parameters were measured. Only E. catarinensis is established in the field in South Africa despite the multiple releases of E. eichhorniae at over 70 sites across the country, and E. catarinensis has established at only 22 of the 45 release sites accessed during this study. This thesis tested climate, interaction with other agents already on P. crassipes, and direct competition between the two mirid species as reasons for the lack of establishment of E. eichhorniae. The results of the country-wide surveys showed that climate and water trophic status were the major determinants in the establishment of E. catarinensis. Most of the establishment was recorded in the warmer regions of the country, however, a few populations of the mirid also established in cooler areas, thus demonstrating a degree of thermal plasticity, and possible microclimates as the mirids persisted at sites shaded by riparian vegetation. Stochastic events such as active herbicide campaigns, winter frosts, droughts and floods were responsible for the absence of the mirid at some sites. At some of the eutrophic sites, despite the abundance of E. catarinensis, plants still proliferated as the water trophic status facilitated plant growth, thus, plants were able to compensate for the damage inflicted by the mirid. A more intensive, monthly, post-release evaluation was conducted on the Kubusi River, Eastern Cape Province between 2016 and 2019. This is regarded as one of the cooler water hyacinth sites. Populations of biological control agents at this site fluctuated seasonally. At this site, cold winters caused frosting of the leaves of P. crassipes with the exception of plants growing under overhanging vegetation that provided a refuge for the mirid. But, cool temperatures in the winter months (May to August) severely reduced the populations of E. catarinensis that required a long recovery phase in spring. The consequence of this was that the plants grew unchecked from the onset of the growing season forming dense mats. Of the four agents at the Kubusi River site, Eccritotarsus catarinensis recovered slowest after winter, with lag phases ranging from two months to several months of the three-year period. The release of a suite of agents has implications on the agents themselves, where interactions between the agents can be important. Interactions between pairs and even multiple agents can have implications for biocontrol, where agents are either complimentary or interfere with each other. In this case, because E. catarinensis recovered the slowest of the four agents at the site, plants were of a poor quality by mid-summer resulting in low mirid populations. Competition in weed biological control could be expected to be strongest between pairs of agents that share the same niche, and this could be the reason why E. eichhorniae failed to establish at sites where E. catarinensis had already been established for several years. When the two mirids were combined in manipulated trials in a polytunnel, populations were lower compared to when the two mirids occurred separately. Under warm conditions, it is likely that E. eichhorniae would be the superior agent compared to E. catarinensis. The evaluations discussed in this thesis highlighted gaps in agent release methodology in multispecies settings, as well as the need for strategic augmentation pre- and post-winter. It is important to release agents that will complement each other rather than compete, therefore, when releasing agents in a multispecies setting, niche differentiation needs to be considered. Here it is concluded that the best practice for dealing with the mirids is that they should be released individually, and at sites that have no other biological control agents in order to ultimately assess their efficacy. Landscape level, long-term monitoring of biological control programmes shows the impact of the control programme at a broader scale and, are far more informative than short-term studies and at fewer sites. Long-term post-release evaluations should be mandatory in biological control programmes. Furthermore, these assessments will help develop new strategies or improve on existing ones, thus achieve greater success in control.
- Full Text:
- Date Issued: 2020
Quantifying ecosystem restoration recovery and restoration practice following the biological control of invasive alien macrophytes in Southern Africa
- Authors: Motitsoe, Samuel Nkopane
- Date: 2020
- Subjects: Salvinia molesta , Ceratophyllum demersum , Nymphaea mexicana , Invasive plants -- Biological control -- South Africa , Aquatic weeds -- Biological control -- South Africa , Restoration monitoring (Ecology) -- South Africa , Biolotical invasions -- Environmental aspects
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167519 , vital:41488
- Description: Invasive alien aquatic plants (IAAP) species are known to have deleterious effects on the freshwater ecosystems they invade. This includes both socio-economic and ecologically important ecosystem goods and services. Thus, IAAP species are declared a serious threat, second only to habitat modification for causing a loss of aquatic biodiversity. Three control methods have been widely applied to control IAAP species invasion globally; mechanical, chemical and biological control. Both mechanical and chemical control methods are considered short-term and expensive, whereas biological control methods are regarded an effective and long-term solution for IAAP species control at the landscape level. But, little is known of the ecological recovery following the biological control of IAAP species, with mechanical control known to have had mixed success and chemical control to have non-targeted effects on aquatic ecosystems, causing harm to wildlife and human well-being. Biological control practitioners measure the success of biological control based on: (1) the biological control agents’ establishment and the negative impacts they impose on the targeted weed; and (2) the weeds biomass reduction and an increase in native macrophytes species. Arguably, measures of biological control success have been subjective and variable across the globe. Although some field studies have demonstrated biological control success to have positive socio-economic returns, there is little literature on ecological benefits. Furthermore, there is limited understanding on ecosystem recovery and possible restoration efforts following the biological control IAAP species, as compared to alien weeds in terrestrial and riparian ecosystems. Thus, this thesis aimed to quantify the ecological recovery i.e. aquatic biodiversity, ecosystem processes and trophic interactions following the management of Salvinia molesta in freshwater ecosystems. The research employed a suite of Before-After Control-Impact mesocosm and field studies to investigate the response of aquatic microalgae, macroinvertebrates and their interactions (food web structure and function) during S. molesta infestation and after mechanical and biological control. The mesocosm experiment (Before invasion, During invasion & After control) showed that both aquatic microalgae and macroinvertebrate diversity indices were reliable biological indicators of S. molesta ecological impacts and recovery following control. The restored treatment (100% S. molesta cover + biological control agents), demonstrated complete aquatic microalgae and macroinvertebrate recovery following biological control, similar to the control treatment (open water), where the degraded/impacted treatment (100% S. molesta cover with no biological control agents) showed a drastic decline in aquatic biodiversity and a complete shift in aquatic biota assemblage structure. Thus, the biological control effort by Cyrtobagous salviniae, the biological control agent for S. molesta, assisted in the recovery of aquatic biota following successful biological control. The field study (four field sites, two sites controlled mechanically and two biologically) investigated water quality, aquatic biodiversity and community trophic interactions (aquatic food web) “before and after” S. molesta control. The study showed a drastic decline in aquatic biodiversity (with three sites showing no record of aquatic macroinvertebrates, thus no biotic interactions during infestation) and poor water quality due to the shade-effect (light barrier due to floating S. molesta mats on the water surface) during the “before” S. molesta control phase. However, following both mechanical and biological control (“after” S. molesta control phase), there was a significant shift in abiotic and biotic ecosystem characteristics as compared to the “before” S. molesta control phase. Thus, rapid ecosystem recovery was apparent as a result of aquatic microalgae and macroinvertebrates recolonisation. Sites showed a normal functioning ecosystem where improved water quality, increased biodiversity, productivity and trophic interactions, was indicative of the return of biologically and functionally important species which were lost during the “before” S. molesta phase. Although the clear water state showed positive outcomes at Westlake River, these were short lived when the system was dominated by a cosmopolitan submerged Ceratophyllum demersum, and later replaced by a floating-leaved emergent IAAP Nymphaea mexicana. Each state was responsible for a significant shift in both biotic and abiotic characteristics, affirming macrophyte abilities to influence aquatic environments structure and functions. Furthermore, this event showed a clear example of a secondary invasion. Thus, a holistic IAAP species management strategy is necessary to restore previously invaded ecosystems and prevent subsequent secondary invasion and ecosystem degradation. In conclusion, the S. molesta shade-effect like any other free-floating IAAP species, was identified as the main degrading factor and responsible for water quality reduction, loss of aquatic diversity and shift in aquatic biota assemblage structure. Following S. molesta removal (or shade-effect elimination), there was a positive response to aquatic ecosystem species abundance, richness, diversity and community structure. Therefore, in combination, aquatic biota recolonisation rate and increases in biological and functional diversity were instrumental in the recovery of ecosystem structure and functions, following the control of S. molesta. Echoing existing literature, this thesis recommends: (1) IAAP species management programmes (mechanical and/or biological control) should not only aim to control the weed but also focus on ecosystems recovery and possible restoration goals; (2) biological control should be used where appropriate to combat free-floating IAAP species in freshwater ecosystems, followed by active introduction of native macrophyte propagules since they are limited by anthropogenic activities; and (3) more freshwater case studies are needed to add to our understanding of IAAP species management and restoration effort incorporating long-term monitoring.
- Full Text:
- Date Issued: 2020
- Authors: Motitsoe, Samuel Nkopane
- Date: 2020
- Subjects: Salvinia molesta , Ceratophyllum demersum , Nymphaea mexicana , Invasive plants -- Biological control -- South Africa , Aquatic weeds -- Biological control -- South Africa , Restoration monitoring (Ecology) -- South Africa , Biolotical invasions -- Environmental aspects
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167519 , vital:41488
- Description: Invasive alien aquatic plants (IAAP) species are known to have deleterious effects on the freshwater ecosystems they invade. This includes both socio-economic and ecologically important ecosystem goods and services. Thus, IAAP species are declared a serious threat, second only to habitat modification for causing a loss of aquatic biodiversity. Three control methods have been widely applied to control IAAP species invasion globally; mechanical, chemical and biological control. Both mechanical and chemical control methods are considered short-term and expensive, whereas biological control methods are regarded an effective and long-term solution for IAAP species control at the landscape level. But, little is known of the ecological recovery following the biological control of IAAP species, with mechanical control known to have had mixed success and chemical control to have non-targeted effects on aquatic ecosystems, causing harm to wildlife and human well-being. Biological control practitioners measure the success of biological control based on: (1) the biological control agents’ establishment and the negative impacts they impose on the targeted weed; and (2) the weeds biomass reduction and an increase in native macrophytes species. Arguably, measures of biological control success have been subjective and variable across the globe. Although some field studies have demonstrated biological control success to have positive socio-economic returns, there is little literature on ecological benefits. Furthermore, there is limited understanding on ecosystem recovery and possible restoration efforts following the biological control IAAP species, as compared to alien weeds in terrestrial and riparian ecosystems. Thus, this thesis aimed to quantify the ecological recovery i.e. aquatic biodiversity, ecosystem processes and trophic interactions following the management of Salvinia molesta in freshwater ecosystems. The research employed a suite of Before-After Control-Impact mesocosm and field studies to investigate the response of aquatic microalgae, macroinvertebrates and their interactions (food web structure and function) during S. molesta infestation and after mechanical and biological control. The mesocosm experiment (Before invasion, During invasion & After control) showed that both aquatic microalgae and macroinvertebrate diversity indices were reliable biological indicators of S. molesta ecological impacts and recovery following control. The restored treatment (100% S. molesta cover + biological control agents), demonstrated complete aquatic microalgae and macroinvertebrate recovery following biological control, similar to the control treatment (open water), where the degraded/impacted treatment (100% S. molesta cover with no biological control agents) showed a drastic decline in aquatic biodiversity and a complete shift in aquatic biota assemblage structure. Thus, the biological control effort by Cyrtobagous salviniae, the biological control agent for S. molesta, assisted in the recovery of aquatic biota following successful biological control. The field study (four field sites, two sites controlled mechanically and two biologically) investigated water quality, aquatic biodiversity and community trophic interactions (aquatic food web) “before and after” S. molesta control. The study showed a drastic decline in aquatic biodiversity (with three sites showing no record of aquatic macroinvertebrates, thus no biotic interactions during infestation) and poor water quality due to the shade-effect (light barrier due to floating S. molesta mats on the water surface) during the “before” S. molesta control phase. However, following both mechanical and biological control (“after” S. molesta control phase), there was a significant shift in abiotic and biotic ecosystem characteristics as compared to the “before” S. molesta control phase. Thus, rapid ecosystem recovery was apparent as a result of aquatic microalgae and macroinvertebrates recolonisation. Sites showed a normal functioning ecosystem where improved water quality, increased biodiversity, productivity and trophic interactions, was indicative of the return of biologically and functionally important species which were lost during the “before” S. molesta phase. Although the clear water state showed positive outcomes at Westlake River, these were short lived when the system was dominated by a cosmopolitan submerged Ceratophyllum demersum, and later replaced by a floating-leaved emergent IAAP Nymphaea mexicana. Each state was responsible for a significant shift in both biotic and abiotic characteristics, affirming macrophyte abilities to influence aquatic environments structure and functions. Furthermore, this event showed a clear example of a secondary invasion. Thus, a holistic IAAP species management strategy is necessary to restore previously invaded ecosystems and prevent subsequent secondary invasion and ecosystem degradation. In conclusion, the S. molesta shade-effect like any other free-floating IAAP species, was identified as the main degrading factor and responsible for water quality reduction, loss of aquatic diversity and shift in aquatic biota assemblage structure. Following S. molesta removal (or shade-effect elimination), there was a positive response to aquatic ecosystem species abundance, richness, diversity and community structure. Therefore, in combination, aquatic biota recolonisation rate and increases in biological and functional diversity were instrumental in the recovery of ecosystem structure and functions, following the control of S. molesta. Echoing existing literature, this thesis recommends: (1) IAAP species management programmes (mechanical and/or biological control) should not only aim to control the weed but also focus on ecosystems recovery and possible restoration goals; (2) biological control should be used where appropriate to combat free-floating IAAP species in freshwater ecosystems, followed by active introduction of native macrophyte propagules since they are limited by anthropogenic activities; and (3) more freshwater case studies are needed to add to our understanding of IAAP species management and restoration effort incorporating long-term monitoring.
- Full Text:
- Date Issued: 2020
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