An empirical analysis of the asset price monetary policy transmission channel in South Africa
- Authors: Du Preez, Wilhelmus Petrus
- Date: 2021-10
- Subjects: Monetary policy South Africa , Banks and banking, Central South Africa , Transmission mechanism (Monetary policy) South Africa , Capital assets pricing model , Securities South Africa , Stocks Prices South Africa , Repurchase agreements South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190318 , vital:44983
- Description: The asset pricing channel of monetary policy transmission breeds an interesting study. There has been an ongoing debate to determine whether monetary policy committees should directly influence the asset pricing channel through changes in the central bank control interest rate or whether monetary policy committees should refrain from targeting the asset pricing channel to meet their policy objectives. The study aims to critically analysis the asset pricing channel, firstly on a global context to set the scene and then to critically focus on the study performed by Muroyiwa et al. (2017) who conducted a study on the South African monetary policy and its interdependence on the stock market through utilisation of an SVAR model. It was concluded that monetary policy does significantly influence stock prices. The purpose of this study is to build on the results which have been formed by Muroyiwa et al. (2017) and to expand on the period under analysis which encapsulates 1999:01 to 2007:12 in order to conclude whether there are conflicting conclusions or conclusions which build on what has already been done. The results from the research shows that when the monetary policy committee decides to influence changes in the repo rate, the repo rate will have a direct effect on the asset markets, the indexes that were used to represent the asset market are the all share index, industrial 15 index and the financial 15 index. Furthermore. The study which has expanded the period under analysis has supplemented and built on the results provided by Muroyiwa et al. (2017). , Thesis (MCom) -- Faculty of Commerce, Economics and Economic History, 2021
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An evolutionary study of legless skinks’ (Acontias Cuvier, 1817) head and vertebrae morphology
- Authors: Evlambiou, Anthony Andreas
- Date: 2021-10
- Subjects: Skinks South Africa , Acontias South Africa , Typhlosaurus South Africa , Acontias Morphology , Acontias Phylogeny , Acontias Evolution , Vertebrae , Skull Growth , Evolutionary developmental biology
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190690 , vital:45018
- Description: Environmental factors and/or processes can produce differences in general shape between individuals or particular parts of individuals. Examples of these biological processes may include ontogenetic development, adaptation to local geographic factors, or long-term evolutionary diversification. An organism is not likely to be able to optimise a single structure for multiple purposes and so trade-offs are likely to occur. An example of such a structure is the cranium, as it can be used for multiple activities such as defensive and sexual behaviour, locomotion, prey capture, and ingestion. Morphological characteristics have historically been used in the description of species. Genetic analyses have gained popularity as species delineation techniques and have been particularly useful in identifying cryptic species, especially among morphological conserved species like legless skinks of the subfamily Acontinae (e.g. Acontias Cuvier, 1817 and Typhlosaurus Weigmann, 1834). However, completely doing away with morphological techniques during species descriptions is not the best option. Therefore, novel methods to identify species, especially those with similar body plans, are needed. In this dissertation, we explore the links between head shape and vertebral number to environmental pressures to determine whether the evolutionary process is driven by environmental pressures (soil or biome) or is retained through ancestry. A novel species/clade delineation linked to vertebral number is also investigated. Head shape was expected to have a close link to the environment and the number of vertebrae was expected to have a closer link to ancestry. The first chapter investigates the drivers behind Acontias head shape evolution using geometric morphometric techniques. We found that environmental pressures did affect the evolution of head shape especially in the “soil” and “biome” categories but further investigation is advised. The second chapter explores the viability of using vertebral counts as a novel method for species and/or clade delineation in Acontias and to determine whether vertebral number can be linked to the environment. Delineating species based on vertebral count is likely not an option, however, delineating clades proved to show promising results. A link between vertebral count and environment was found in Acontias with larger bodied species occurring in different environments to smaller body species. In conclusion, the genus Acontias is difficult to delineate morphologically. Genetic sequence analyses can indicate differences and delineate the species. Even though there were differences in morphology based on environmental factors, it is not sufficient to delineate this subfamily alone. Further research is advised and this dissertation provides a good basis to work with. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2021
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Building the field component of a smart irrigation system: A detailed experience of a computer science graduate
- Authors: Pipile, Yamnkelani Yonela
- Date: 2021-10
- Subjects: Irrigation efficiency Computer-aided design South Africa , Irrigation projects Computer-aided design South Africa , Internet of things , Machine-to-machine communications , Smart water grids South Africa , Raspberry Pi (Computer) , Arduino (Programmable controller) , ZigBee , MQTT (MQ Telemetry Transport) , MQTT-SN , XBee
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/191814 , vital:45167
- Description: South Africa is a semi-arid area with an average annual rainfall of approximately 450mm, 60 per cent of which goes towards irrigation. Current irrigation systems generally apply water in a uniform manner across a field, which is both inefficient and can kill the plants. The Internet of Things (IoT), an emerging technology involving the utilization of sensors and actuators to build complex feedback systems, present an opportunity to build a smart irrigation solution. This research project illustrates the development of the field components of a water monitoring system using off the shelf and inexpensive components, exploring at the same time how easy or difficult it would be for a general Computer Science graduate to use hardware components and associated tools within the IoT area. The problem was initially broken down through a classical top-down process, in order to identify the components such as micro-computers, micro- controllers, sensors and network connections, that would be needed to build the solution. I then selected the Raspberry Pi 3, the Arduino Arduino Uno, the MH-Sensor-Series hygrometer, the MQTT messaging protocol, and the ZigBee communication protocol as implemented in the XBee S2C. Once the components were identified, the work followed a bottom-up approach: I studied the components in isolation and relative to each other, through a structured series of experiments, with each experiment addressing a specific component and examining how easy was to use the component. While each experiment allowed the author to acquire and deepen her understanding of each component, and progressively built a more sophisticated prototype, towards the complete solution. I found the vast majority of the identified components and tools to be easy to use, well documented, and most importantly, mature for consumption by our target user, until I encountered the MQTT-SN (MQTT-Sensor Network) implementation, not as mature as the rest. This resulted in us designing and implementing a light-weight, general ZigBee/MQTT gateway, named “yoGa” (Yonella's Gateway) from the author. At the end of the research, I was able to build the field components of a smart irrigation system using the selected tools, including the yoGa gateway, proving practically that a Computer Science graduate from a South African University can become productive in the emerging IoT area. , Thesis (MSc) -- Faculty of Science, Computer Science, 2021
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Coaches perspective on the positional demands of school boy (u18/u19) rugby players during a 70-minute match
- Authors: Barnard, Devon Vernon
- Date: 2021-10
- Subjects: Rugby football coaches South Africa , Rugby Union football players South Africa , Rugby football injuries South Africa , School sports South Africa , School sports Coaching South Africa , School sports Physiological aspects
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190089 , vital:44962
- Description: INTRODUCTION: Rugby union has been one of the team sports that has grown over the last few years, and the margin between success and failure for teams has become narrower as the years have gone by. The game consists of players going through different movements such as sprints and/or collisions, that vary in intensity. The demands that face schoolboy rugby players are not well understood, and the lack of research in this area leads to a potential lack of understanding of the perceptions that coaches have about the demands that schoolboys are faced with during a match. OBJECTIVE: Therefore, the purpose of this study was to determine the perceptions that the schoolboy rugby coaches had surrounding the demands that their players are faced with during a 70-minute match. There were a few secondary purposes of the study, such as determining which school, government or private, had a better understanding or perception of the demands, as well as comparing strength and conditioning coaches’ perceptions to regular coaches’ perceptions. METHODS: This was a cross-sectional, descriptive study to determine the coach’s perceptions. These perceptions were collected through an online questionnaire, which consisted of ten sections and 74 questions. The questionnaire was piloted on a subject group with different types of experience. Coaches from the top 100 schools list were contacted through their school website. RESULTS: The questionnaire had a 72% response rate: 41 responses from 51 emails. The 41 responses consisted of 23 government schools and 18 private schools, which included 11 of the top 20 rugby schools in South Africa, with most responses coming from the Eastern Cape (41.5%). Results indicated that a government school’s coaches had significantly (p<0.05) more experience than private schools’ coaches, and that they had significantly (p<0.05) better academic qualifications than private schools. However, strength and conditioning coaches had significantly (p<0.01) better academic qualifications than coach, whereas coaches had significantly (p<0.01) better coaching qualifications than strength and conditioning coaches. CONCLUSION: “Experience has been shown to be a potential reason for what is described as a good coach”. This is also another potential reason why government schools are a dominant force in schoolboy rugby in South Africa. However, this is also coupled with better qualifications showing the dual importance of both experience and education. This study has shown that coaches, within a South African cohort, overall had good general perception of the demands of school level rugby matches. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2021
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Echogenic liposomes for ultrasound-triggered drug delivery
- Authors: Izuchukwu, Ezekiel Charles
- Date: 2021-10
- Subjects: Liposomes , Drug delivery systems , Colon (Anatomy) Cancer Treatment , Transmission electron microscopy , Fourier transform infrared spectroscopy , Liquid chromatography , Echogenic liposomes , Ultrasound-triggered drug delivery
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/10962/188997 , vital:44805
- Description: Colorectal cancer is one of common cancers worldwide. It is the third most diagnosed cancer and the second leading cause of death. The use of 5-fluorouracil (5-FU) alone or in a chemotherapy regime has been the effective treatment of colorectal cancer patients. The efficacy of 5-FU in colorectal cancer treatment is significantly limited by drug resistance, gastrointestinal, and bone marrow toxicity through high-level expression of thymidylate synthase, justifying a need to improve its therapeutic index. Liposomes are colloidal membranes comprising of one or more lipid bilayers enclosing an aqueous core. They have been used to improve the therapeutic index of many anti-cancer drugs by changing drug absorption, elongating biological half-life, reducing metabolism, and reducing toxicity to healthy tissues. Echogenic liposomes are specifically designed to respond to external triggering like ultrasound stimulation by entrapping a gas or an emulsion that can vaporize. A liposome's unique property is that it can entrap both hydrophobic and hydrophilic substances simultaneously in the lipid bilayer and the aqueous core, respectively. These stimuli-responsive liposomes can be triggered externally with ultrasound, to release the chemotherapeutic cargo only at the required site. This research aims to formulate echogenic liposomes encapsulating 5-FU for potential ultrasound triggered release (echogenic). Liposome formulations wereprepared with lipid composition of crude soybean lecithin and cholesterol by thin-filmhydration method and the drug was passively loaded in the formulation. The 5-FU loadedliposomes were evaluated by dynamic light scattering (DLS) for particle size, polydispersityindex, and zeta potential and transmission electron microscopy (TEM) for morphology.Encapsulated liposomal formulations were also evaluated using physicochemical techniquesincluding thermogravimetric analysis (TGA), differential scanning calorimetry (DSC),Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). Theencapsulation efficiency and release kinetics were studied using a validated high-performanceliquid chromatography (HPLC) method. Echogenic properties were explored by entrapping abiocompatible gas (argon) at the same time as the drug (5-FU) using a pressure/freezemethodology. The liposomal formulations were typically spherical with a size of about 150 nmand encapsulation efficiency of 62%. Low-frequency ultrasound (20 kHz) was used to triggerthe drug release from the complete formulation at 10%, 15%, and 20% amplitude and exposuretime of 5 min and 10 min. The rate of drug release from the nano-carrier was a function of theultrasound amplitude and exposure time and reached a maximum of 65% release under theconditions investigated. The cumulative release was investigated, with and without theapplication of ultrasound. It was demonstrated that the application of ultrasound resulted in complete release (99%) after 12 h while this dropped to 70% without ultrasound. These results are encouraging for optimizing ultrasound parameters for triggered and controlled release of the 5-FU, for conditions such as the management of cancer where low-power ultrasound can be applied. , Thesis (MSc) -- Faculty of Science, Chemistry, 2021
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Ecological infrastructure importance for drought mitigation in rural South African catchments: the Cacadu Catchment case example
- Authors: Xoxo, Beauten Sinetemba
- Date: 2021-10
- Subjects: Sustainable Development Goals , Water security South Africa , Remote sensing , Watershed restoration South Africa , Restoration ecology South Africa , Ecosystem services South Africa , SDG 15.3.1
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/191203 , vital:45070
- Description: Water scarcity is recognised as one of the significant challenges facing many countries, including South Africa. The threat of water scarcity is exacerbated by the coupled impacts of climate and anthropogenic drivers. Ongoing droughts and continued land cover change and degradation influence the ability of catchments to partition rainwater runoff, thereby affecting streamflow returns. However, quantifying land degradation accurately remains a challenge. This thesis used the theoretical lens of investing in ecological infrastructure to improve the drought mitigation function in rural catchments. This theoretical framework allows for a social-ecological systems approach to understand and facilitate science-based strategies for promoting ecosystem recovery. Specifically, this study aimed to explore the role and benefit of ecological infrastructure for improving drought mitigation, and consequently, water security for rural communities. Thus, this study sought to assess the consequences of human actions to catchment health status using the 15th Sustainable Development Goal indicator for the proportion of degraded land over the total land area as a surrogate. Secondly, hydrological modelling was used to describe how different land covers influence catchment hydrology, which related to how ecological infrastructure enables drought risk-reduction for mitigation regulation. Finally, this study developed a spatial prioritisation plan for restoration to improve drought mitigation for four focal ecological infrastructure (EI) categories (i.e. wetlands, riparian margins, abandoned agricultural fields and grasslands). The focal EI categories were selected for their importance in delivering water-related ecosystem services when sustainably managed. Chapter 1 sets the scene (i.e. provides the study background) and Chapter 2 provides a review of the literature. In Chapter 3, the recently released global GIS toolbox (TRENDS.EARTH) was used for tracking land change and for assessing the SDG 15.3.1 degradation indicator of i.e. Cacadu catchment over 15 years at a 300 m resolution. The results showed a declining trend in biomass productivity within the Cacadu catchment led to moderate degradation, with 16.79% of the total landscape degraded, which was determined by the pugin using the one-out, all-out rule. The incidence of degradation was detected in middle reaches of the catchment (i.e. S10F-J), while some improvement was detected in upper reaches (S10A-C) and lower reaches (S10J). In Chapter 4, a GIS-based Analytic Hierarchical Process (AHP) based on community stakeholder priorities, open-access spatial datasets and expert opinions, was used to identify EI focal areas that are best suitable for restoration to increase the drought mitigation capacity of the Cacadu catchment. The collected datasets provided three broad criteria (ecosystem health, water provision and social benefit) for establishing the AHP model using 12 spatial attributes. Prioritisation results show that up to 89% of the Cacadu catchment is suitable for restoration to improve drought mitigation. Catchments S10B-D, and S10F, S10G and S10J were highly prioritised while S10A, S10E and S10H received low priority, due to improving environmental conditions and low hydrological potential. Areas that were prioritised with consideration for local livelihoods overlap the areas for drought mitigation and form a network of villages from the middle to lower catchment reaches. Prioritised restoration areas with a consideration of societal benefit made up 0.56% of wetlands, 4.27% of riparian margins, 92.06% of abandoned croplands, and 51.86% of grasslands. Chapter 5 reports on use of the Pitman groundwater model to help understand the influence of land modification on catchment hydrology, and highlight the role of restoration interventions. The Cacadu catchment is ungauged, therefore the neighbouring Indwe catchment was used for parameter transfer through a spatial regionalisation technique. Results suggest that degradation increases surface runoff and aggravates recharge reduction, thereby reducing streamflow during low flow periods. In areas where there is natural land cover recovery, the Pitman Model simulated similar dry season streamflow to the natural land cover. Combining the outcomes from the three assessments allowed the study to highlight the role and benefits of ecological infrastructure in terms of drought mitigation. Study findings were interpreted to make recommendations for the role and benefit of ecological infrastructure for drought mitigation at a landscape scale and tertiary catchment level, within the context of available management options. The results support the notion that multiple science data sources can promote investments in ecological infrastructure. However, better spatial and temporal resolution datasets at a national level are still needed to improve the accuracy of studies such as the one outlined in this thesis. The study recommends adopting better ecosystem protection approaches and collaborative governance at multiple levels to reduce the vulnerability of rural communities to drought impacts. , Thesis (MSc) -- Faculty of Science, Institute for Water Research, 2021
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Effects of elevated temperature, rainfall and soil nutrients on acacia mearnsii invasion
- Authors: Kharivha, Tshililo
- Date: 2021-10
- Subjects: Acacia mearnsii , Acacia mearnsii Effect of high temperatures on South Africa , Acacia mearnsii Climatic factors South Africa , Plant invasions South Africa , Invasive plants , Climatic changes South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/189997 , vital:44954
- Description: Climate change is associated with the risk of plant invasion hence a better understanding of the effects of elevated temperature, precipitation and soil nutrients on dominant invasive plants is needed for effective ecological planning. This study was set out to: (i) examine how elevated temperature (±2°C increase), (ii) high (above local average) and low (below local average) precipitation, (iii) elevated soil nutrient content (increase in soil N), and (iv) a combination of the above manipulations affects germination and growth of Acacia mearnsii, a dominant invasive plant in South Africa. The study further evaluated how the above-mentioned treatments affect soil chemical properties following A. mearnsii germination and growth. The above-mentioned specific objectives were tested under manipulated greenhouse conditions over six experimental months. The results indicated that the above-mentioned climate change scenarios have the potential to facilitate germination and growth of the invasive species A. mearnsii, and this is likely to proliferate its invasion in future. Results showed that seed germination was significantly high under all climate change manipulation treatments (˃50%) with highest seed germination recorded under high rainfall treatment (64%). Plant height was significantly higher under high temperature and high rainfall treatments throughout all the experimental months, though it was lowest under high nitrogen and combined treatment with high rainfall. The numbers of branches were high under higher temperature and low rainfall treatments than under high rainfall, high nitrogen and both combined treatments of low and high rainfall. Relative to the control, plants grown under climate change scenarios increased their root lengths, but this varied across different treatments. Total dry biomass was relatively high under high temperature treatment (0,7 g). Lower plant dry biomass was observed under low and high rainfall treatments (0,4 g), high nitrogen and combined treatments with both low and high rainfall treatments (0,1 g). Concerning the effects of climate change scenarios on soil chemical properties, soil pH levels were significantly higher after A. mearnsii germination and growth than before the experiment was setup. Soil resistivity was significantly higher in climate change treatments receiving nitrogen and combined treatments of low rainfall than other treatments and the soils before experiment. Soil total P was significantly higher in all the climate change treatments after A. mearnsii germination experiment than the before experiment soils. Soils receiving high temperature, high nitrogen, and combined treatment of low rainfall had significantly higher soil total N than other treatments and the before experiment soils. Soil total C was significantly higher in soils receiving high temperature, high nitrogen, and combined treatment of low rainfall after A. mearnsii germination than other treatments and before experiment soils. The findings suggest that future climate change scenarios of increased temperature and rainfall with soil nutrients could considerably enhance growth and germination success of the invasive plant A. mearnsii. Similarly, climate change scenarios could enhance some soil nutrient properties, which in turn, is likely to give the invasive plant A. mearnsii a germination and growth advantage. These results are the first in South Africa to show that future climate changes have the potential to facilitate A. mearnsii germination and growth, making it more invasive. The findings have implications for invasive plants management, especially action for managing the plant through clearing of the plant in sensitive ecosystems (e.g. riparian systems). , Thesis (MSc) -- Faculty of Science, Environmental Science, 2021
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Evaluating the physiological, morphological and nutritional effects of elevated atmospheric CO2 and drought on select South African maize cultivars
- Authors: Bopape, Tebadi Mamadiga
- Date: 2021-10
- Subjects: Corn Varieties South Africa , Dry farming South Africa , Corn Effect of atmospheric carbon dioxide on South Africa , Corn Effect of drought on South Africa , Corn Morphology , Corn Nutrition , Corn Physiological effect
- Language: English
- Type: Master theses , text
- Identifier: http://hdl.handle.net/10962/189020 , vital:44807
- Description: Thesis embargoed. Expected Release date October 2022. , Thesis (MSc) -- Faculty of Science, Botany, 2021
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Evaluation of Megabruchidius tonkineus (Coleoptera: Chrysomelidae: Bruchinae), a candidate biological control agent for Gleditsia triacanthos L. (Fabaceae) in South Africa
- Authors: Salgado Astudillo, Sara Elizabeth
- Date: 2021-10
- Subjects: Honey locust South Africa , Honey locust Biological control South Africa , Invasive plants Biological control South Africa , Biogeography South Africa , Biogeography Climatic factors South Africa , Megabruchidius tonkineus South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/188386 , vital:44749
- Description: Gleditsia triacanthos L. (Fabaceae) (honey locust) is a fast-growing, deciduous tree indigenous to the United States of America. Introduced around the world as an ornamental tree, it has become invasive in a number of countries. Where it is invasive, G. triacanthos competes and replaces indigenous species; it creates dense stands along watercourses, posing a significant environmental threat. In South Africa, G. triacanthos is regarded as one of the country’s fastest spreading weeds. Gleditsia triacanthos produces numerous seeds contained in large hanging pods. Once dislodged from the pods, the seeds are dispersed by birds and mammals, including livestock, which eat the pods. It has been suggested that the seeds should be the target for biological control programme. Some invasive alien plant species are characterised by their ability to spread and establish in new ecosystems because they tolerate a wide range of environmental conditions. In order to predict areas of likely invasion, species distribution models (SDMs) are used to identify areas climatically suitable for their invasion, so enabling better targeted control of the plant species. Gleditsia triacanthos adapts to a wide range of climates and soil types, and tolerates salinity, drought and frost. Currently primarily restricted to the Grassland Biome of South Africa, G. triacanthos has doubled its distribution area in the past 15 years, and it is not known how far the species will spread. In this study we used two different modelling programmes, CLIMEX and MaxEnt, to predict areas where G. triacanthos could find favourable growing conditions; both SDMs showed that most of the country is suitable for G. triacanthos and that it will probably continue to spread, if left unmanaged, into new bioregions, such as the Karoo. In South Africa, the Asian seed-feeding bruchid, Megabruchidius tonkineus (Pic, 1914) (Coleoptera: Chrysomelidae: Bruchinae) has been recorded in the plant’s seed pods and has been considered as a biological control agent. The insect was not released as part of a formal biological control programme and neither host-specificity nor impact studies were conducted on the species prior to its discovery. In 2017 a decision was made to re-consider its status as a Abstract biological control agent until further details of its biology, host specificity, and impact on the seeds of G. triacanthos in South Africa were available. This study shows that Megabruchidius tonkineus has established across the entire G. triacanthos population in South Africa damaging approximately 9% of seeds. Laboratory studies show that, Megabruchidius tonkineus completes its larval development in the seeds of G. triacanthos in about 66.80 ± 0.6880 SE days before eclosing. In addition, the adult females oviposit on the following Fabaceae species: Arachis hypogaea, Albizia, julibrissin, Cicer arietinum, Pisum sativum, Dipogon lignosus, Peltophorum africanum, Podalyria buxifolia Senegalia burkei, Umtiza listerina and Vachellia sieberiana. However, larval development was limited to G. triacanthos. It is concluded that the seed-feeding beetle is not a threat to native Fabaceae species in South Africa, however, it does not damage enough G. triacanthos seeds to be considered a valuable biological control agent at this stage, and additional seed-feeding biological control agents should be considered to reduce the number of G. triacanthos seeds entering the environment. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2021
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Exploring affordances and hindrances when using Grade 8 Physical Science learners’ home language to mediate learning of chemical bonding
- Authors: Shilongo, Wilhelm Nghidiputate
- Date: 2021-10
- Subjects: Science Study and teaching (Secondary) Namibia , Code switching (Linguistics) , Chemical bonds , Education, Bilingual Namibia , Native language and education Namibia , Socio-cultural theory
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/191079 , vital:45056
- Description: It seems code-switching from English to learners’ home language is a regular practice in Namibian schools. That is, science teachers mediate specific challenging science topics such as chemical bonding through code-switching from English to learners’ home languages. Based on anecdotal evidence and on Grade 10 Examiners’ Reports for previous years, chemical bonding is one of the challenging key concepts in Physical Science, and yet it is an important concept in the study of chemistry. Therefore, the purpose of this interventionist study was to investigate how the use of learners’ home language enables and/or constrains Grade 8 Physical Science learners’ sense making of the topic, chemical bonding. The study is a convergent parallel mixed method case study and was underpinned by an interpretive paradigm. It was conducted with 17 Grade 8 learners in a rural school, where I teach, in the Oshana region. I used a diagnostic test to elicit learners’ prior knowledge on this topic, a post-intervention test, observation, reflections, focus group interview and stimulated recall interviews as my data collection methods. I used Vygotsky’s (1978) Socio-Cultural Theory as my theoretical framework to help explain how learners make sense of the concept of chemical bonding when learners’ home language is used in a Physical Science class. To analyse the quantitative data obtained, I used a descriptive statistics and inferential statistics method. In the descriptive statistics, data were compiled into graphs, tables, and other visual representations, while for the inferential statistics, a t-test was used to establish the significant difference of the data collected. On the other hand, a thematic approach to analyse the qualitative data was employed to come up with sub-themes and themes. The findings of the study revealed that learners’ home language stimulates learners’ interest to learn new concepts, and enhances learners’ understanding and participation during the science lessons. Another finding of the study revealed that learners’ prior knowledge in chemical bonding contained some misconceptions. Furthermore, the findings of the study also revealed that learners learn best when their prior knowledge are elicited before they are introduced to new concepts in their science classrooms. The study recommends that learners’ home language should be incorporated into Physical Science lessons, especially when the teachers are teaching challenging concepts such as chemical bonding. , Thesis (MEd) -- Faculty of Education, Education, 2021
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Green Synthesis of HIV-1 Protease Inhibitors
- Authors: Hartley, Shaun Neil
- Date: 2021-10
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190145 , vital:44967
- Description: Thesis embargoed until October 2022 , Thesis (MSc) -- Faculty of Science, Chemistry, 2021
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In a novel landscape, in the Eastern Cape, South Africa, what are the key vegetation resources that support livestock production?
- Authors: Liversage-Quinlan, William Peter
- Date: 2021-10
- Subjects: Mzimvubu River Watershed (South Africa) , Livestock productivity South Africa Mzimvubu River Watershed , Range ecology South Africa Mzimvubu River Watershed , Land degradation South Africa Mzimvubu River Watershed , Vegetation dynamics South Africa Mzimvubu River Watershed , Novel landscapes
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190053 , vital:44959
- Description: The Tsitsa River is the largest remaining river that is without a dam in South Africa, and so the government has pledged to install two major dams for irrigation and hydroelectricity, under the Mzimvubu Water Project. These dams are intended to supply water and electricity to surrounding communities as well as the large neighbouring urban areas of Mthatha and Tsolo. However, large areas of the Tsitsa Catchment (~494 000 ha) are degraded, posing the possibility that if these dams were to be created, they would inevitably fill with sediment and silt within a few decades. To address these issues, the Tsitsa Project was established in 2014 to co-ordinate actions by government, developers and local communities in order to restore the landscape condition and prevent soil erosion, as well as to improve the livelihoods of the people who live there (Fabricius, Biggs, & Powell, 2016). Research into the landscape condition as well and the environmental interactions with local communities are therefore highly necessary in order to guide restoration practices to their best effect. The economic necessity for livestock production in the catchment indicates its urgent need for a better understanding of its current effects on the catchment landscape and vegetation. The overall goal of this study is to contribute to the knowledge needed to manage the area in a sustainable way. This paper will therefore examine the key vegetation resources that support livestock production in an area of the Tsitsa River Catchment, by describing the various types that exist, and by determining their relative production levels of usable livestock forage. , Thesis (MSc) -- Faculty of Science, Institute for Water Research, 2021
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Investigation of sediment buffering function of the Gatberg Floodplain Wetland in the upper Tsitsa River Catchment, South Africa
- Authors: Pakati, Sibuyisele Sweetness
- Date: 2021-10
- Subjects: Sedimentation and deposition South Africa Eastern Cape , Sediment transport South Africa Eastern Cape , Floodplain morphology South Africa Eastern Cape , Wetlands South Africa Eastern Cape , Suspended sediments South Africa Eastern Cape , Floods South Africa Eastern Cape , Fluvial geomorphology South Africa Eastern Cape , Floodplain plants South Africa Eastern Cape , Inundation depth
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190792 , vital:45028
- Description: Floodplain wetlands are important components of river systems that provide various ecosystem services such as sediment buffering. These wide and often expansive storage areas have a substantial impact on downstream water quality by trapping sediment and storing ‘contaminants’ adhered to sediment thus improving water quality. The planned construction of the Ntabelanga and Lalini Dams in the Tsitsa River Catchment has been proposed; however, due to the steep landscapes and erodible soils, this promotes high erosion rates that can potentially reduce the lifespan of the proposed dams. The existing wetlands in the Tsitsa River Catchment have therefore been identified as key sediment buffers that can reduce sediment transport, but the effectiveness of these buffers is poorly researched. This study attempts to investigate the current sediment buffering function of the Gatberg Floodplain Wetland over one wet season (August 2019 to August 2020). Time integrated samplers were installed above and below the wetland to determine relative sediment volume and character coming in and out of the wetland. Five transects were surveyed across the wetland width to evaluate the topography and vegetation characteristics. Surface sediment samples on the floodplain were taken at key morphological features along each transect and along the river longitudinal profile to determine organic content, particle size, and type of stored sediment. Astro turf mats were deployed on targeted transects and on key floodplain features to determine sediment accumulation rates. Field measurements of vegetation parameters (height, density, and stem diameter) were taken to calculate vegetation-induced hydraulic roughness to understand possible sedimentation feedbacks. The relative sediment volume coming into the wetland was greater than that leaving the wetland. This implies that some of the sediment is buffered within the wetland. An approximate proportion of 73% trapping efficiency of the incoming sediment was buffered within the floodplain wetland during the wet season. This accumulated approximately 4 tons within the wetland over the monitoring frame. Bed particle size in the longitudinal profile increased with distance downstream, this was due to localized tributary and hillslope inputs. Inundation depth varied across the floodplain wetland with deeper inundation depths at the head of the wetland than at the bottom; where particle size was larger with an increase in water level depth. This may be linked to both high stream velocities and variability of the floodplain topography. However, the observed trends were inconclusive and uncertain. Stronger correlations with particle size were shown by vegetation roughness (b* = 0.41) and distance from the channel (b* = -0.38). Flood benches and banks had a coarser D50 particle size than back swamps and oxbows. Coarser sediment in flood benches are associated with proximity to the sediment-laden water that experiences abrupt flow velocity changes, while finer material in oxbows are due to minimal flow velocities which reduce with distance from the channel. Finer particles remain in suspension and are carried aloft for longer periods at very low velocities. Therefore, particle size decreased with distance from the channel due to longer travel distances and high surface area relative to weight. Further results showed that finer surface sediment particle size was associated with high vegetation roughness whilst coarser material was associated with low roughness. This was due to vegetation geometry and type or changes in flow velocity and energy. Grassy vegetation induced finer particle size than shrubby vegetation that has a greater line spacing. Furthermore, vegetation roughness varied over the wet season; roughness was highest in late summer and low in early summer. Low roughness was due to fire occurrence in the study area which resulted in a decrease in biomass. Increasing vegetation roughness can be due to increased flood events, and the introduction of non-perennial species; which can increase sediment accumulation rates. Although studies have shown that vegetation density is the most essential factor affecting flow resistance and sedimentation processes; vegetation height and stem diameter for this study area seem to contrast these observations and rather may be the most significant contributing factors in sedimentation. This concluded that vegetation density may not always be the most essential component in sedimentation processes. Sediment particle size was inversely proportional to organic content; finer particle size are more cohesive and more capable of carrying organics. Regions further away from the channel such as oxbows with stable moisture conditions favour plant growth and soil formation thus are susceptible to high organic content. Flood benches are closer to the channel, thus have coarser material and fluctuating moisture conditions that have unstable high water flow velocities. High sediment accumulation rates on flood benches and oxbows is due to high connectivity to sediment-laden water and high hydroperiods or high residence time for sediment accumulation in oxbows. Sediment accumulation rate was shown to be a function of particle size itself (b* = 0.67) rather than the expected vegetation roughness. Although a true representation of sediment accumulation rates in the Gatberg Wetland was limited by the disturbance of astro turf mats by animals and possibly by high flooding events; the wetland can be regarded as a good sediment buffer as some sediment was stored (e.g. up to 48,04 kg/m2 in flood benches) within the wetland over the monitoring period. , Thesis (MSc) -- Faculty of Science, Geography, 2021
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Is there an inverse-u relationship between financialisation and investment?: South Africa’s stock market capitalisation in comparative perspective
- Authors: Owen, Michael Robert
- Date: 2021-10
- Subjects: Monetary policy South Africa , Globalization , Economics South Africa , Capital movements South Africa , Stock exchanges South Africa , Investments South Africa , Economic development South Africa , South Africa Economic conditions 1991-
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/191084 , vital:45058
- Description: In recent decades, the growth and fluctuations in the financial sector have become increasingly disjointed from events in the real economy. There has been a dramatic increase in global market integration and globalisation since the 2008 financial crisis. The argument presented in the thesis shows perspective from the two general debates in Economics. The Orthodox view, which suggests there is an efficient flow of resources between people and institutions over time, supports the argument that there is a positive relationship between financialisation and economic growth. Alternatively, the Heterodox view argues that financial liberalisation fails to anticipate market effects and requires intervention in order to limit negative consequences. More recent studies have proposed an inverse-U theory between financialisation and investment, which suggests that there is a threshold, above which further financial development and financial globalisation has detrimental effects on the real economy. With South Africa being a developing country that is highly reliant on capital inflows to finance the economy, there is space for more acute research to investigate whether South Africa has surpassed this threshold. This study focusses on one aspect of this relationship, namely the relationship between Stock Market Capitalisation and Investment. It uses panel data analysis and other methods to explore whether such an inverse-U relationship exists internationally, and whether South Africa is suffering from the effects of financialisation. , Thesis (MCom) -- Faculty of Commerce, Economics and Economic History, 2021
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Mobilising stories about cultural beliefs and practices on traditional foods to contextualise the topic on nutrition in a Grade 6 township class
- Authors: Nuntsu, Sipho Nimrod
- Date: 2021-10
- Subjects: Culturally relevant pedagogy South Africa , Science Study and teaching (Elementary) South Africa , Nutrition Study and teaching (Elementary) South Africa , Traditional ecological knowledge South Africa , Storytelling , Reasoning , Contiguity Argumentation Theory (CAT) , South African Curriculum Assessment and Policy Statement (CAPS) , Socio-cultural theory
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190940 , vital:45043
- Description: The South African Curriculum Assessment and Policy Statement (CAPS) document stipulates that science teachers should integrate indigenous knowledge (IK) into their science teaching. The rationale for this is to contextualise the content and make science accessible and relevant to learners. Despite these ideals, however, CAPS seems to be silent on how science teachers should go about doing this. Instead, it assumes that all teachers know how to integrate IK in their science teaching. As a result, many teachers are still not sure of how to integrate IK into their science classrooms. Such rhetoric and tension between curriculum formulation and implementation triggered my interest to do a study on how to mobilise stories about cultural beliefs and practices of traditional foods to mediate learning of nutrition in a Grade 6 Natural Sciences township class. The study was underpinned by an interprevist paradigm complemented with an Ubuntu paradigm to enhance explanations. Within these paradigms, a qualitative case study research design was adopted. It was conducted at Mdoko Primary school (pseudonym) in a semi-urban community in the Amathole West district of the Eastern Cape. The participants were 34 Grade 6 learners (15 boys and 19 girls), a Grade 6 Natural Sciences teacher who was my critical friend, and two expert community members. To generate data, I used a focus group discussion, group activities, classroom observations, and learners’ reflective journals. Vygotsky’s socio-cultural theory and Ogunniyi’s Contiguity Argumentation Theory (CAT) were used as theoretical and analytical frameworks, respectively. A thematic approach to data analysis was employed. That is, data were analysed inductively to identify sub-themes and subsequently similar sub-themes were grouped together to form themes. The two expert community members’ presentations equipped my learners with stories on cultural beliefs and practices that were used in the olden days (past) and how such stories are used in our days (present). For instance, the findings of this study revealed that women during menstruation must not drink amasi as it is believed that it would prolong the menstruation time. The findings also revealed that eating of amaqanda and inside meat by youths should be minimised as it is believed that it can stimulate their sex hormones. It also revealed that there is no relevance to science that men eating imifino would be weak among other men who do not eat them. The implications for this study is that science teachers should make some efforts to integrate IK in their teaching to make science accessible and relevant to their learners. To achieve this, the study thus recommends that science teachers should find ways of tapping into the cultural heritage and wisdom that is possessed by the expert community members to enable learners to cross the bridge from home to school. , Thesis (MEd) -- Faculty of Education, Education, 2021
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Modification of Gelatin-Methacrylate, Hyaluronic-Methacrylate and Poly(ethylene) glycol Diacrylate hydrogel bioinks towards the additive manufacturing of articular cartilage
- Authors: Barwick, Matthew William
- Date: 2021-10
- Subjects: Cartilage Diseases , Cartilage Regeneration , Articular cartilage Diseases , Chondrogenesis , Stem cells , Scanning electron microscopy , Fourier transform infrared spectroscopy , Three-dimensional printing , Gelatin-Methacrylate , Hyaluronic-Methacrylate , Poly(ethylene) glycolDiacrylate , Hydrogel bioinks , Real-Time Quantitative Cell Analysis (RTCA) , Bioprinting
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/191181 , vital:45068
- Description: Cartilage degradation is most commonly associated with Rheumatoid arthritis and Osteoarthritis, affecting millions of people worldwide. Joint transplants commonly use titanium alloys, which have a shelf life of between 10-15 years. Although the titanium transplant restores partial mobility, side effects such as inflammation, swelling, faulty implants, and metal poisoning in some cases resulting from the transplant. The use of additive manufacturing of articular cartilage sheds new, innovative prospects for joint replacements. This study sets out to formulate and characterize five different hydrogel types towards the additive manufacturing of articular cartilage. Chondrogenic and Adipogenic differentiation was carried out on two separate adipose-mesenchymal stem cell lines A270620-01A, and A311019-02T and validation and efficiency of the differentiation and chondrogenic gene expression was carried out using Alcian Blue stain, Oil Red O stain and Quantitative Reverse Transcription PCR (RT-qPCR). Hydrogel formulation and characterisation of 10 % Gelatin-methacryloyl (GelMA), 10 % Poly (ethylene) glycol diacrylate (PEGDA), 5 % GelMA/5 % PEGDA, 10 % GelMA/0.5 % Hyaluronic Acid Methacrylate (HAMA) and 10 % PEGDA/0.5 % HAMA was carried out through swelling and degradation ratios, surface area and porosity characterisation using Scanning Electron Microscopy (SEM). Hydrogel component and spectroscopic analysis were carried using Real-Time Quantitative Cell Analysis (RTCA) and Fourier-transform Infrared Spectroscopy (FTIR) analysis for each formulated hydrogel's chemical characterisation. Three-dimensional printing (3D) of 10 % PEGDA/0.5 % HAMA and 5 % GelMA/5 % PEGDA was performed using the Zortrax INKSPIRE Resin Ultra-Violet (UV) LCD Desktop 3D Printer. Hydrogel sterility and cell viability were carried out for each hydrogel type using fluorescence microscopy. Both A270620-01A and A311019-02T cell lines showed adipogenic and chondrogenic differentiation ability, with A311019-02T cell line showing greater chondrogenic differentiation of Alcian blue staining. The A270620-01A cell line resulted in a greater collagen gene expression based on the RT-qPCR results. The hydrogel 10 % GelMA showed the greatest swelling ratio of 1260 % in DPBS and 1192 % in DMEM. A significant difference between hydrogel swelling and swelling with Dulbecco's Phosphate Buffered Saline (DPBS) and Dulbecco’s Modified Eagle Medium (DMEM) was observed. The 10 % PEGDA hydrogel had the greatest degradation ratio of 59 % mass remaining, where the 10 % GelMA/0.5 % HAMA showed the least amount of degradation with a mass remaining at 91 %. The 10 % GelMA showed the greatest porosity will the largest pore size of 14 μm in diameter. Hydrogel component and spectroscopic analysis showed no cytotoxic effects for the visible light photoinitiator used to polymerize the hydrogel and no cytotoxic effects for the concentrations used in chondrogenic differentiation. The FTIR analysis showed partial gelatin and hyaluronic acid modification with methacrylic anhydride; however, the distinction between the hybrid hydrogels and single polymer hydrogels could not be made effectively. UV and ethanol washing showed to completely sterilise the hydrogel disks from any contaminants, making them suitable for tissue culture. The cell viability analysis showed the 10 % GelMA/HAMA having the highest cell viability of 77.3 % using 5000 cells/disk and 89.64 % viability using 50 000 cells/disk over a 7-day incubation period. Overall, the combination of two polymers, GelMA and HAMA, has good potential as a 3D hydrogel scaffold towards additive manufacturing of articular cartilage. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2021
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Nonlinear optical properties of metal free thio alkyl and tert-butyl phenoxy phthalocyanine
- Authors: Joseph, Otto
- Date: 2021-10
- Subjects: Nonlinear optics , Phthalocyanines , Time-dependent density functional theory , Magnetic circular dichroism , Reverse saturable absorption (RSA) , Real Time Dependent Density Functional Theory (RT-TDDFT)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190712 , vital:45021
- Description: This work reports on the nonlinear optical properties of tetra - and octa substituted phthalocyanines (Pcs) utilising pentane thiol and 4-tertbutyl phenol as substituents. Their nonlinear absorption coefficient (𝛽) and absorption cross sections were determined using the Z-scan technique with a 10 ns pulse laser at 532 nm. The molecular second order hyperpolarizability Im[γ] was observed and the following Im[γ] trend was obtained for 𝛼-H2Pc(SC5H11)4 isomers, 5.93 ×10−31 (Cs) 2.24×10−32(D2h) > 1.21×10−32(C4h) > 1.05×10−32 (C2v) esu, respectively, in chloroform. Symmetry was seen to have an effect on the observed reverse saturable absorption (RSA) response. Based on the five level model rate equation nonlinear fit of the RSA response curves and Real Time Time Dependant Density Functional Theory (RT-TDDFT) results, the singlet excited state population dynamics was found to play a significant role in producing the observed Im[γ] trend. , Thesis (MSc) -- Faculty of Science, Chemistry, 2021
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Numerical evolution of plane gravitational waves
- Authors: Hakata, Jonathan
- Date: 2021-10
- Subjects: Gravitational waves , Space and time , Einstein field equations , de Sitter metric , Perturbed spacetime
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190248 , vital:44977
- Description: Unlike electromagnetic waves, gravitational waves self interact. This interaction is non-linear and can have very interesting properties which effect the curvature of space-time. A gravitational plane wave collider, implemented in the Python package COFFEE [20] that been developed in recent years by the Otago relativity group and implements the method of lines, can be reliably used to study this self-interaction. This was shown to work well numerically as profounded by Frauendiener, Stevens and Whale in 2014 [24]. For this reason, COFFEE will be used to study these gravitational wave propagations and subsequently collisions. The Einstein field equations are formulated as a well-posed initial boundary value problem (IBVP) in the Friedrich-Nagy gauge [26] and due to the large class of boundary conditions admitted by this framework, a variety of investigations into the propagation of plane gravitational waves could be carried out. This study focuses on the propagation of plane gravitational waves in the de Sitter (dS) space-time, which is the maximally symmetric solution of the Einstein’s vacuum field equations with a positive cosmological constant λ. There is substantial cosmological evidence that our universe is asymptotically de Sitter, yet no work, analytical nor numerical, has been done on gravitational plane waves propagating on such a space-time, mainly due to the increased complexity from the non-vanishing λ. Firstly, it is found analytically that with an arbitrary cosmological constant λ and a non-vanishing energy momentum tensor, the constraints will propagate. This means that we still have a wellposed IBVP, which is nontrivial since the Friedrich-Nagy gauge has only been shown to lead to a wellposed IBVP without matter [26]. Using this system, we consider one ingoing wave propagating on said space-time in vacuum. The area of the ingoing wave profile is varied and inferences are made about the different phenomena that arise in the curvature of space-time during the evolution. It is found that there exists a critical value of the wave’s area, ac, whereby taking the area below this value the system asymptotes to its initial state, and above the system diverges, indicating the presence of a singularity. Furthermore, we define an expansion parameter H to measure how the gravitational waves influence the accelerated expansion, generalising (numerically) results of Tsamis and Woodard. , Thesis (MSc) -- Faculty of Science, Mathematics, 2021
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Nurturing an identity of land custodianship of young people through a biodiversity learning programme: The Moletele Youth Learning Programme
- Authors: Mponwana, William Oupa
- Date: 2021-10
- Subjects: Sustainable biodiversity South Africa Mopani District Municipality , Association for Water and Rural Development (South Africa) , Land reform beneficiaries South Africa Mopani District Municipality , Agricultural development projects South Africa Mopani District Municipality , Land use Study and teaching South Africa Mopani District Municipality , Identity (Psychology) Social aspects South Africa Mopani District Municipality , Identity (Psychology) in youth South Africa Mopani District Municipality , Moletele Youth Learning Programme , Moletele Communal Property Association (CPA) , RESILIM-O
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/10962/188820 , vital:44789
- Description: This study was an exploration of value creation through scaffolding in a context specific learning programme with young people within the Moletele Communal Property Association (CPA) who own land through the land reform programme in South Africa. The study aimed to support the development the identity of land custodianship amongst young people by developing their skills and competencies in a situated learning context. The study also informed ongoing support work through the RESILIM-O resilience building project implemented by the Association for Water and Rural Development (AWARD) to help the Moletele Community Property Association to better understand the implications of biodiversity and land use for developing sustainable beneficiation projects and planning. A case study approach was adopted through a learning programme with young people as an in-depth exploration of the complexities of land and biodiversity. Data were collected during sessions in the learning programme where we used a spiral framework to facilitate learning focusing on four themes for primary data: knowledge competence, technical skills, self-organization and collective agency which are linked to building an identity of custodianship. Semi structured interviews were conducted at the end of the learning programme as a secondary data source. The study found that scaffolding remained an important process throughout the learning programme, and different types of scaffolding was needed to support the learning processes and themes. Furthermore, different types of value were created over time through the interactions and activities of the learning programme. The study also found that young people have a desire to participate in their communal property association. Lastly the study revealed that building custodianship as an identity is a continuous, long term process that needs commitment of time, resources and supportive learning processes, for an identity of custodianship to emerge. , Thesis (MEd) -- Faculty of Education, Education, 2021
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Phylogeography and reproductive isolation of the brown mussel, Perna perna, on the South African coastline
- Authors: Barker, Cassandra
- Date: 2021-10
- Subjects: Mexilhao mussel South Africa , Phylogeography South Africa , Mexilhao mussel Reproduction , Mexilhao mussel Genetics , Intertidal organisms South Africa , Mexilhao mussel Climatic factors South Africa , Cytochrome oxidase , Mitochondrial DNA , Haplotype network
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190041 , vital:44958
- Description: Maintenance of a species’ abundance and adaptive potential is partially dependent on its genetic diversity. Distinct genetic lineages within a species can differ significantly in their resistance and resilience to environmental pressure. An assessment of such differences is key to grasp the adaptive potential of a species. Physical environmental conditions are significant determinants of the distribution of species and their genetic lineages. Under climate change scenarios, the assessment of the temporal stability of the spatial distribution of genetic structure has important consequences for conservation as it offers key insights into the adaptive potential and evolutionary capacity of a species. This thesis investigated the phylogeography and reproductive isolation of Perna perna, the brown mussel. This species is an ecologically and economically important intertidal mussel on South Africa’s coastline. It was determined in 2007 that there were two distinct genetic lineages of P. perna present on South Africa’s coastline, the Eastern and Western lineage. This thesis compared mitochondrial DNA between samples collected in 2007 and samples collected in 2019 to determine differences in genetic structure of P. perna over time. This thesis further investigated the reproductive timing of the two lineages to determine if this contributes to the maintenance of the genetic structure of this species. The results show that there is a shift in the distribution of the Eastern lineage since 2007, an increase in the range of the overlap region, and there was a change in genetic diversity in the form of private haplotypes between 2007 and 2019. The lineages have unsynchronised spawning and reproductive patterns are more closely linked to changes in temperature. While the lineages have shown slight distributional changes over time, the influence of environmental conditions and the predicted changes in sea surface temperatures could see a change in future populations’ fitness and dispersal. Thus, changes in sea surface temperature could affect reproductive timing and the future genetic stability of the species. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2021
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