Photosynthetic gas exchange responses to light, temperature, carbon dioxide and water stress, and changes in photosynthetic pigments to light and water stress in two cultivars of Hordeum vulgare L
- Logie, Malcolme Ronald Ruxton
- Authors: Logie, Malcolme Ronald Ruxton
- Date: 1992
- Subjects: Plants -- Photorespiration , Plants -- Transpiration , Botanical chemistry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4210 , http://hdl.handle.net/10962/d1003779
- Description: The gas exchange responses of two cultivars of Hordeum vulgare L., to light, temperature, CO₂ and water stress were investigated in the laboratory. The optimum temperature for net CO₂ assimilation was found to be 25°C and 22.5°C for cv. Clipper and cv. Dayan respectively. Net CO₂ assimilation was reduced at 30°C in cv. Dayan. At low light intensity the highest quantum yield efficiency was 0.051 mol.mol⁻¹ at 30°C for cv. Clipper, and 0.066 mol.mol⁻¹ at 20°C for cv. Dayan. At the same temperature, cv. Clipper had a higher water use efficiency than cv. Dayan, but stomatal conductance for cv. Dayan was higher than cv. Clipper. Stomatal limitation to CO₂ was lowest at the optimum temperature for CO₂ assimilation in both cultivars. Stomata limited CO₂ assimilation in cv. Clipper to a larger degree than in cv. Dayan. Relative stomatal limitation for cv. Clipper at 25°C was 0.280 ± 0.010, and for cv. Dayan at 22.5°C was 0.028 ± 0.011. Short-term exposure to elevated CO₂ concentrations increased CO₂ assimilation in both cultivars, but more so for cv. Clipper. Transpiration rate at elevated CO₂ partial pressures were higher in cv. Dayan than in cv. Clipper. At very high CO₂ (860 μmol.m⁻²s⁻¹) partial pressure water use efficiency in cv. Clipper was higher than cv. Dayan, but at low CO₂ partial pressures water use efficiency in cv. Dayan was higher than cv. Clipper. Water stress reduced the relative leaf water content and net CO₂ assimilation in both cultivars. Cultivar Dayan was more tolerant to water stress, and CO₂ assimilation in this cultivar was less affected by water stress. In both cultivars water stress increased the concentration of chlorophyll a, chlorophyll b, and chlorophyll a+b. The chlorophyll a:b ratio remained relatively constant throughout the stress period. No correlation between relative leaf water content and total carotenoid concentration was observed.
- Full Text:
- Date Issued: 1992
- Authors: Logie, Malcolme Ronald Ruxton
- Date: 1992
- Subjects: Plants -- Photorespiration , Plants -- Transpiration , Botanical chemistry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4210 , http://hdl.handle.net/10962/d1003779
- Description: The gas exchange responses of two cultivars of Hordeum vulgare L., to light, temperature, CO₂ and water stress were investigated in the laboratory. The optimum temperature for net CO₂ assimilation was found to be 25°C and 22.5°C for cv. Clipper and cv. Dayan respectively. Net CO₂ assimilation was reduced at 30°C in cv. Dayan. At low light intensity the highest quantum yield efficiency was 0.051 mol.mol⁻¹ at 30°C for cv. Clipper, and 0.066 mol.mol⁻¹ at 20°C for cv. Dayan. At the same temperature, cv. Clipper had a higher water use efficiency than cv. Dayan, but stomatal conductance for cv. Dayan was higher than cv. Clipper. Stomatal limitation to CO₂ was lowest at the optimum temperature for CO₂ assimilation in both cultivars. Stomata limited CO₂ assimilation in cv. Clipper to a larger degree than in cv. Dayan. Relative stomatal limitation for cv. Clipper at 25°C was 0.280 ± 0.010, and for cv. Dayan at 22.5°C was 0.028 ± 0.011. Short-term exposure to elevated CO₂ concentrations increased CO₂ assimilation in both cultivars, but more so for cv. Clipper. Transpiration rate at elevated CO₂ partial pressures were higher in cv. Dayan than in cv. Clipper. At very high CO₂ (860 μmol.m⁻²s⁻¹) partial pressure water use efficiency in cv. Clipper was higher than cv. Dayan, but at low CO₂ partial pressures water use efficiency in cv. Dayan was higher than cv. Clipper. Water stress reduced the relative leaf water content and net CO₂ assimilation in both cultivars. Cultivar Dayan was more tolerant to water stress, and CO₂ assimilation in this cultivar was less affected by water stress. In both cultivars water stress increased the concentration of chlorophyll a, chlorophyll b, and chlorophyll a+b. The chlorophyll a:b ratio remained relatively constant throughout the stress period. No correlation between relative leaf water content and total carotenoid concentration was observed.
- Full Text:
- Date Issued: 1992
The interactive effects of light, temperature and CO₂/O₂ ratios in photosynthesis of Coix lachryma-jobi L
- Authors: Mjwara, Jabulani Michael
- Date: 1992
- Subjects: Coix , Plants -- Effect of light on , Plants -- Effect of oxygen on , Plants -- Effect of carbon dioxide on , Photosynthesis , Plants -- Effect of temperature on
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4216 , http://hdl.handle.net/10962/d1003785
- Description: A portable infra red gas analyzer was used to investigate the interactive effects of light, temperature, and CO,jO, ratios under controlled environmental conditions in an attempt to model gas exchange characteristics of Coix Iachryma-jobi L. Plotting light response curves as a function of temperature (20, 25 30 and 35°C) revealed no sign of light saturation even at a photosynthetic photon flux density (PPFD) close to 2000 !Lmol m-' sol. High net assimilation rates (A) of approximately 24 !Lmol CO, m"s'! were realized at 30-35°C. Assimilation (A) versus internal CO, partial pressure (C,) curves showed a steep rise with increase in C, but saturated at approximately 150 (JLII-!) and all the results, either in the absence or presence of 0" showed a similar response under all temperature regimes. C. Iachryma-jobi exhibited low CO, compensation points cr ) between 0 and 10 JLlI-! under similar experimental temperatures and either at 0 or 21%0,. The slopes of double reciprocal plots of llA versus llCi, were nearly identical and crossed the yintercept at almost identical points under all 0, concentrations. These data indicate first; that there was no apparent 0, inhibition and second; indicated that the apparent inhIbitor constant (K,) for 0, at the site of carboxylation did not change with increase in [OJ from 0 to 21% oxygen. These observations were further confirmed by results obtained from the analysis of apparent carboxylation efficiency (CE, as defined as the slope of response of A to increasing CO,), as no inhibition of A with increase of [OJ occurred. These characteristics were consistent with typical features of C,photosynthesis. The absence of 0, inhibition and low r values indicated that an efficient CO, concentrating mechanism which eliminates photorespiration exists in C. Iachryma-jobi. At the light microscope level, leaf anatomy exhibited typical C, structure viz. bundle sheath with large chloroplasts and this sheath is further surrounded by a radiate Kranz mesophyU cells. Furthermore the anatomical features suggested that C. wchryma-jobi was an NADP-ME species. Stomatal conductance (g,) to assimilation (g,/A) indicated an increase in A with decrease in g" an essential feature of improving water use efficiency (WUE), but one which drastically reduces CO, diffusion rate. The physical lintitation (stomatal lintitation, t) to CO, diffusion under various [0,] and temperatures, but constant PPFD, did not exhibit statistically significant change in t values at either 0 or 21% a, within each temperature regime, however there was a marked decrease in t as the plant approached its optimum photosynthetic temperature.
- Full Text:
- Date Issued: 1992
- Authors: Mjwara, Jabulani Michael
- Date: 1992
- Subjects: Coix , Plants -- Effect of light on , Plants -- Effect of oxygen on , Plants -- Effect of carbon dioxide on , Photosynthesis , Plants -- Effect of temperature on
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4216 , http://hdl.handle.net/10962/d1003785
- Description: A portable infra red gas analyzer was used to investigate the interactive effects of light, temperature, and CO,jO, ratios under controlled environmental conditions in an attempt to model gas exchange characteristics of Coix Iachryma-jobi L. Plotting light response curves as a function of temperature (20, 25 30 and 35°C) revealed no sign of light saturation even at a photosynthetic photon flux density (PPFD) close to 2000 !Lmol m-' sol. High net assimilation rates (A) of approximately 24 !Lmol CO, m"s'! were realized at 30-35°C. Assimilation (A) versus internal CO, partial pressure (C,) curves showed a steep rise with increase in C, but saturated at approximately 150 (JLII-!) and all the results, either in the absence or presence of 0" showed a similar response under all temperature regimes. C. Iachryma-jobi exhibited low CO, compensation points cr ) between 0 and 10 JLlI-! under similar experimental temperatures and either at 0 or 21%0,. The slopes of double reciprocal plots of llA versus llCi, were nearly identical and crossed the yintercept at almost identical points under all 0, concentrations. These data indicate first; that there was no apparent 0, inhibition and second; indicated that the apparent inhIbitor constant (K,) for 0, at the site of carboxylation did not change with increase in [OJ from 0 to 21% oxygen. These observations were further confirmed by results obtained from the analysis of apparent carboxylation efficiency (CE, as defined as the slope of response of A to increasing CO,), as no inhibition of A with increase of [OJ occurred. These characteristics were consistent with typical features of C,photosynthesis. The absence of 0, inhibition and low r values indicated that an efficient CO, concentrating mechanism which eliminates photorespiration exists in C. Iachryma-jobi. At the light microscope level, leaf anatomy exhibited typical C, structure viz. bundle sheath with large chloroplasts and this sheath is further surrounded by a radiate Kranz mesophyU cells. Furthermore the anatomical features suggested that C. wchryma-jobi was an NADP-ME species. Stomatal conductance (g,) to assimilation (g,/A) indicated an increase in A with decrease in g" an essential feature of improving water use efficiency (WUE), but one which drastically reduces CO, diffusion rate. The physical lintitation (stomatal lintitation, t) to CO, diffusion under various [0,] and temperatures, but constant PPFD, did not exhibit statistically significant change in t values at either 0 or 21% a, within each temperature regime, however there was a marked decrease in t as the plant approached its optimum photosynthetic temperature.
- Full Text:
- Date Issued: 1992
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