A systematic literature review of the current approaches to dream work: global and local trends
- Authors: Feris, Eric-John Ricardo
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435440 , vital:73157
- Description: Dreaming is an inherent and universal aspect of human existence that offers a profound window into the realms of consciousness and cognition. It has captivated the human imagination for centuries, prompting various methods and theories to decipher its significance. Global research underscores the enduring importance of dreams in psychotherapy, expressed through diverse methodologies, techniques, and theoretical models. Curiously, within the South African context, a cultural landscape rich in traditional healing, there exists a noticeable lack of research concerning the utilisation of dreams in therapy. This systematic literature dove into 30 articles which remained after applying the systematic literature review strict inclusion and exclusion criteria of the literature searches. From these articles, the review delved into the multifaceted world of dream work, investigated prevailing approaches and themes both on a global scale and within the South African context, and addressed three key research questions: (1) What themes can be identified from a review of current approaches to dream work globally? (2) What themes can be identified from a review of current approaches to dream work locally? (3) What are the practical implications for working with dreams according to global and local themes? A thematic analysis of the literature identified three overarching themes: (1) Psychological approaches to dream work, (2) Psychiatric and medical approaches to dream work, and (3) Socio-cultural approaches to dreams. These findings highlight the necessity of recognising cultural nuances and, underscores the importance of developing a flexible, culturally sensitive framework for dream work, and advocates for the adaptation of global dream work methodologies into culturally specific approaches, fostering psychotherapeutic benefits tailored to the South African context. The research serves as a stepping stone to further investigation into the techniques and approaches of dream work in South Africa, ultimately contributing to the broader field of dream analysis. , Thesis (MA) -- Faculty of Humanities, Psychology, 2024
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Aptamer-based biosensor for prostate specific antigen detection using cobalt phthalocyanine-exfoliated graphite composites
- Authors: Benise, Emihle
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434850 , vital:73110
- Description: The work focuses on the development of biosensors and their use for the detection of prostate specific antigen (PSA). Four cobalt phthalocyanines (CoPcs) complexes: (1) cobalt tetra pyridyloxy phthalocyanine, (2) cobalt tetra acetamidophenoxy phthalocyanine, (3) cobalt tris(acetamidophenoxy) mono benzoic acid phthalocyanine, and (4) cobalt tris(acetamidophenoxy) mono propionic acid phthalocyanine, an exfoliated graphite (EG), and aptamer are used to make probes for PSA detection. Each complex is π-π stacked onto the EG to form EG-CoPc(π-π) hybrid which was used to modify a glassy carbon electrode (GCE). EG and CoPc were also used to modify the GCE sequential (seq) with CoPc on top to give GCE-EG-CoPc(seq). For the detection PSA, PSA specific aptamer was either sequential added or covalently linked to complexes 3 and 4 on the modified electrodes and was only sequentially added onto complexes 1 and 2 modified electrodes. Electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) were the techniques used for the detection of PSA. The electrodes were found to be selective in bovine serum albumin, glucose and cysteine and stable when 50 DPV scans were run. Electrodes gave good % recovery when human serum was spiked with different PSA concentrations. , Thesis (MSc) -- Faculty of Science, Chemistry, 2024
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Elucidation of the roles of the carbonic anhydrase enzymes, CanA & CanB, in the physiology of Mycobacterium smegmatis
- Authors: Jackson, Gabriella Teresa
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435299 , vital:73145
- Description: The bacterial pathogen Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis (TB) and one of the leading infectious causes of death globally. The success of Mtb as a pathogen depends on its ability to detect and respond to a variety of physical and chemical stresses it encounters during infection of its human host. These environmental stresses include shifts in temperature, oxygen concentration, osmolarity and nutrient availability. Mtb is, in addition, exposed to changes in pH and CO2 concentration in the intracellular and extracellular environments it inhabits, which the bacterium has to adapt to in order to ensure its growth, survival and/or persistence during infection. Carbonic anhydrases (CAs) are a widely distributed family of enzymes that catalyse the reversible hydration of carbon dioxide (CO2) to bicarbonate (HCO3−) in the reaction: CO2 + H2O ⇄ HCO3− + H+. In microbes, CA activity is important for the activity of enzymes involved in carbon fixation as well as for maintaining pH homeostasis. Mtb is known to express three CAs, encoded by the Rv3588c, Rv1284 and Rv3273 genes (canA, canB and canC, respectively). The role(s) of these CA enzymes in the physiology of Mtb and other mycobacterial species, such as Mycobacterium smegmatis (Msm), has not been elucidated to date. To gain insights into the function of the CanA and CanB enzymes in mycobacterial species, we generated both canA and canB knockdown (KD) and knockout (KO) mutants in the fast-growing mycobacterial species, Msm, and analysed their growth phenotypes under several growth conditions where CA activity is known to be required. Notably, Msm lacks the CanC homologue, which makes it an ideal surrogate to focus on CanA and CanB. The Msm canA KD mutant was found to display a growth defect following anhydrotetracycline (ATc)-mediated gene silencing at atmospheric (low) CO2 concentrations [~0.035% CO2 (v/v)]. The growth defect could be rescued by incubating cells at physiological (high) CO2 concentrations [~5% CO2 (v/v)] or by supplementing the growth media with either HCO3− or the metabolic end-products of certain HCO3−-dependent-carboxylase enzymes at low CO2 concentrations. The ability of these compounds to rescue the growth of the canA KD mutants was, however, dependent on the extent of ATc-mediated gene silencing, suggesting that the canA gene is required for Msm growth at both low and high CO2 concentrations. This was confirmed by our findings that canA could only be genetically inactivated when a second copy of the gene was provided on the chromosome in trans, regardless of the CO2 concentration used. In contrast to our observations for canA, no differences in the growth phenotypes of the Msm wild type (WT) and canB KD or knockout (KO) mutant strains were observed following silencing or inactivation of the canB gene at either low or high CO2 concentrations or different pH values. These observations suggest that, in contrast to canA, the canB gene is dispensable for the growth of Msm under standard laboratory growth conditions. The canB KO mutant strain, nevertheless, displayed a slight decrease in its ability to form biofilms when compared to the WT strain, which could be restored by genetic complementation. CanB activity may, therefore, be required to promote bacterial growth and/or survival under biofilm conditions where CO2 diffusion into cells is limited, a phenomenon that has recently been observed in other microbes. Further studies are required to confirm the role of CanB in biofilm formation and to determine how the different CA enzymes cooperate to promote the growth and survival of mycobacterial species in the various environments they are known to inhabit. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2024
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English morphological awareness and reading comprehension in deaf and hearing grade 3 to 7 learners from Lesotho
- Authors: Tšehla, Puleng Magret
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/${Handle} , vital:73169
- Description: There is a noticeable literacy crisis observed in both Deaf and hearing learners from Lesotho. This study investigates the English Morphological Awareness and reading comprehension of 26 Deaf and 82 hearing learners enrolled in grades 3 to 7 in two schools in Lesotho. This study employs a correlational cross-sectional quantitative design. Each participant completed two literacy assessment tasks: a reading comprehension task and a Morphological Awareness task. The Morphological Awareness task encompassed five subtasks that assessed the learners’ inflectional, derivational, and compound awareness. The results of these assessments are analysed through appropriate statistical analyses. In addition, errors made by the Deaf and hearing learners on the literacy assessments are compared and analysed. This analysis determines the types of errors made by each group and identifies the factors that influence these errors. The performance of both groups in terms of task scores, in general, was low. Deaf learners and hearing learners’ performance on the tasks was similar. This outcome contradicts most previous studies, which indicate that Deaf learners typically exhibit lower levels of Morphological Awareness development and reading comprehension compared to their hearing counterparts. The results of this study also suggest that there is a statistically significant relationship between Morphological Awareness and reading comprehension in both groups. Finally, Deaf and hearing learners made similar errors on the tasks. There was some evidence of influence from both the Deaf and hearing learners’ first languages (Sesotho and Lesotho Sign Language, respectively). The study demonstrates the need for more explicit morphological instruction to improve both Deaf and hearing learners’ literacy. , Thesis (MA) -- Faculty of Humanities, Linguistics and Applied Language Studies, 2024
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High-altitude temporary systems and macroinvertebrate composition in La Nina cycle, Maloti-Drakensberg
- Authors: Moyo, Sibusisiwe
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/434963 , vital:73119
- Description: The Maloti-Drakensberg Mountain is an important water catchment area for South Africa and Lesotho. It is rich in biodiversity in terms of endemic flora and fauna and has a high density of temporary wetlands in the form of Afromontane tarns and rock pools. There is, however, limited information on the distribution of macroinvertebrates in these ecosystems and environmental factors that drives their composition. To that end, this study investigated macroinvertebrate diversity and the effect of environmental factors on community assemblage in the Maloti-Drakensberg Mountain temporary wetlands, specifically tarns and rock pools. The main aims were to describe the abundance, diversity, and distribution of macroinvertebrates in temporary wetlands in the Maloti-Drakensberg Mountain. Furthermore, it aimed to investigate the effect of environmental variables on habitat structuring, spatial variations, and macroinvertebrate community composition in tarns and rockpools in the Maloti-Drakensberg Mountain. Seventy temporary wetlands (29 tarns and 41 rock pools) were surveyed over a once-off sampling (3 March – 7 April 2022). The wetlands were from 3 provinces including Eastern Cape, Free State, and KwaZulu-Natal. 15278 individual macroinvertebrates from 13 orders, 35 families, and 46 genera were collected and identified. Species accumulation curves incorporating both tarns and rock pools showed that observed results were significantly lower than Chao 1 and Chao 2 estimates meaning that the sampled sites were not enough to provide a comprehensive picture of the biodiversity in the region. This was also observed for rock pools when the temporary wetlands were analysed separately, however, the Chao 1 and Chao 2 values for tarns were almost equal to the observed values implying that sampled sites were enough to give a detailed picture of macroinvertebrate composition in tarns. Tarns and rock pools were significantly different in terms of species richness and Shannon-Wiener diversity index but showed no significant differences for relative abundance and Pielou’s evenness. Of the 35 families identified, 19 were considered core taxa with Dytiscidae and Corixidae being the most dominant. Additionally, there was moderate Nestedness metric based on Overlap and Decreasing Fill (NODF) value of 33.05369, with 32 out of 35 families being idiosyncratic. Predators were the dominant feeding group with omnivores being the least common. Non-Metric multi-Dimensional Scaling (NMDS) and PERMANOVA results showed that tarns and rock pools supported distinctly different macroinvertebrate communities per wetland type and location respectively. Physicochemically, tarns and rock pools were significantly different in terms of water temperature, depth, area, and chlorophyll-a concentration with tarns exhibiting higher values. Temporary wetlands in the sampled localities differed in terms of pH, EC, DO, water temperature, altitude, and phosphate. Principal Component Analysis showed that tarns were positively associated with area and depth whereas rock pools with phosphate. Akaike’s Information Criterion (AICc) selection showed that area, pH, and water temperature were significantly influencing community assemblage in temporary wetlands. Furthermore, area significantly influenced all four measured biodiversity indices: relative abundance, species richness, Pielou’s evenness and Shannon-Wiener Index, with water temperature also influencing relative abundance and altitude, species richness. Overall, the species composition showed that the sites were sampled later in the hydroperiod as evidenced by the dominance of active dispersers. The extended distribution of Prosthetops gladiator in the Eastern Cape highlights the need for extensive surveys to provide valuable insight into the distribution patterns of macroinvertebrates in the region. Furthermore, the interplay between physicochemical variables and biodiversity indices emphasized area, water temperature and pH as significant factors. However, their limited explanatory power suggests that obscure factors also influence the diversity cementing the need for further exploration of these enigmatic systems. Taxonomic challenges, especially regarding large branchiopods, hindered precise species distribution and conservation status determination. This study is the first to provide a comprehensive list of macroinvertebrates from temporary wetlands in the Maloti-Drakensberg Mountain. These results establish a valuable baseline for future biological monitoring studies, particularly in assessing species composition and responses to anthropogenic activities. It is a timeous study considering the sensitivity of these systems to climate change as they act as sentinel sites hence the need for foundational data on which to make future projections. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2024
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Home is in the wandering
- Authors: Purdon, Bradley Michael
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435547 , vital:73167
- Description: Submitted in partial fulfilment of the requirements for the degree of Master of Arts in Creative Writing , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2024
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Identifying the factors that influence parents and caregivers to commit child maltreatment: systematic review
- Authors: Dipholo, Mamdlalose Emily
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435421 , vital:73156
- Description: Child maltreatment, encompassing various forms of abuse and neglect, poses a significant global public health concern. This study provides a comprehensive examination of the multifaceted dimensions of child maltreatment, including physical, emotional, and sexual abuse, as well as neglect. The impact of maltreatment on the developing child is profound, resulting in a range of adverse physical and mental health outcomes, developmental delays, behavioural challenges, and social difficulties. The aim of this study was to comprehensively review and synthesise peer-reviewed research on the factors influencing parents and caregivers in committing child maltreatment. This systematic review identified the multifaceted factors influencing parents and caregivers to engage in child maltreatment. Using a social ecological framework, ten key themes emerged, namely poverty and lack of access to services, intergenerational transmission of abuse, childhood trauma, substance abuse, domestic violence, neighbourhood violence, cultural practices, social factors, children’s individual factors, and caregivers' mental health concerns. Personal history, caregiver’s mental health, substance abuse, poor awareness of self-protection, and gender emerged as significant individual-level contributors. Non biological caregivers and domestic violence were identified as relationship-level factors. At the community level, poverty, lack of access to services, unemployment, and neighbourhood violence were found to impact child maltreatment. Cultural values and the intergenerational transmission of abuse were reported as influential societal-level factors. The study underscores the complex interplay of personal, environmental, and cultural influences in child maltreatment, highlighting the need for comprehensive strategies to address this critical issue. , Thesis (MA) -- Faculty of Humanities, Psychology, 2024
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It's all in your head
- Authors: Namukuta, Sonia Charity Sajjabi
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435536 , vital:73166
- Description: My thesis is a collection of short stories that delve into the multifaceted nature of death, with a specific emphasis on murder, violence, death and their profound aftermath, including themes of loss, grief, and trauma. These stories offer diverse perspectives, narrated by perpetrators, voiceless victims, and those left to pick up the shattered pieces. Additionally, some stories are seen through the eyes of those tasked with handling the bodies. Often set in unnamed locations, they delve into imaginary and fantastical worlds, while remaining grounded in recognisable situations and spaces. I draw inspiration from popular and genre fiction such as horror, crime fiction and true crime stories, but approach my writing from a psychological lens, employing stylistic experimentation to challenge readers' expectations. The power of silence is a recurring motif. Rather than focusing on unearthing facts or revealing the "truth" like crime fiction often does, my narratives delve into what isn't or at times cannot be told — the unsayable. Some of my stories explore silence inherent in violence, grief, and the inability to articulate one's experience in the face of a violent act or a life prematurely ended. Others delve into the silence of untold stories and the dark secrets of the perpetrators. By exploring these contrasting perspectives, I aim to offer a nuanced exploration of death and its aftermath. The writing styles of Stephen Graham Jones, Lydia Davis, and Kuzhali Manickavel influence my work. Lydia Davis, known for her mastery of very short, flash fiction, ranging from single sentences to a paragraph or two, inspires me with her precise observations of the human condition. Her minimalist prose, carefully selecting and arranging words and sentences, encapsulates the power of less-is-more storytelling. Manickavel creates surreal yet tangible worlds, combining idiosyncratic, intense and eerie elements with unfiltered expression. Drawing from her ability to blur the lines between the surreal and the real, I infuse my stories with a sense of disquieting authenticity. Stephen Graham Jones stands out for his ability to explore morbid themes in a compelling and unconventional manner. His experimentation with horror fiction tropes, the visceral realism of his prose, and his complex characters inspire me to capture the unsettling feeling that something dreadful has occurred without explicitly detailing the facts and intricacies. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2024
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The feasibility of task-shifting prolonged exposure for Primary Care (PE-PC) for PTSD within a low resource community in the Eastern Cape
- Authors: Shabangu, Princess Noxolo Nonhlanhla
- Date: 2024-04-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/435479 , vital:73161
- Description: Although high levels of trauma exposure potentiate an alarming prevalence of PTSD, increasing the need for trauma-focused treatment, the South African health system is unable to care for the population. The South African healthcare system contains a range of systemic and structural inefficiencies, including suboptimal care and shortages of healthcare professionals. PE-PC offers a resource-constrained mental healthcare system solution by integrating an evidence-based intervention (EBI) in primary care settings. Likewise, the present study acknowledges the ratio of mental healthcare professionals to patients in low- and middle-income countries (LMIC). It explores task shifting to expand mental healthcare to primary care settings. The study follows qualitative healthcare design research to explore the experiences and perceptions of the two cohorts of trauma support workers (TSW) from Rhode University and the Joza community in Makhanda. The feasibility of the PE-PC intervention in Makhanda was illustrated through the data obtained from the TSWs' experiences and perceptions of the intervention's utility. The present study presents an essential contribution to the sparse research conducted on treating PTSD in LMIC with shortages in healthcare professionals. The thematic findings look at the trauma support workers (TSWs) role, including some of its challenges, to explore the feasibility of interventions. Some of the themes highlighted unpack the perceived utility of task-shifting in low-resource contexts, the limitations thereof and considerations for future research. Notwithstanding the challenges, the data from the present study indicates that PE-PC can be feasibly task-shifted in low-resource contexts such as Makhanda. , Thesis (MA) -- Faculty of Humanities, Psychology, 2024
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