Fossil Shell Flour supplementation in the diet of Dohne Merino rams: effects on feed acceptability and heat stress tolerance
- Mwanda, Lwazi https://orcid.org/0000-0001-9503-6150
- Authors: Mwanda, Lwazi https://orcid.org/0000-0001-9503-6150
- Date: 2020-02
- Subjects: Sheep--Feeding and feeds , Animal feeding , Fossils
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/22567 , vital:52464
- Description: The objective of the current study was to evaluate the effects of feeding Dohne Merino rams with various inclusion levels of Fossil Shell Flour (FSF) as an additive supplement on feed preference and heat tolerance response of Dohne Merino rams. The FSF is used as an animal feed additive and is known to be rich in minerals. A total of twenty-four Dohne Merino rams (average 19.8 ± 0.43 kg body weight) were used to determine the feed preference of varying inclusion levels fossil shell flour (0g FSF/kg, 20g FSF/kg, 40g FSF/kg and 60g FSF/kg). The preference was assessed using feed intake and coefficient of preference. The feed intake, co-efficient of preference and physical attributes of feed (such as colour, smell and texture) was measured. The feed intake and coefficient of preference were higher in rams supplemented 60g FSF/kg (P < 0.05). The physical attributes of the experimental diets were adjudged based on the colour, smell and texture. The colour of experimental diets were brown in 0g FSF/kg while in 20g FSF/kg, 40g FSF/kg and 60g FSF/kg the colour of experimental diets were light brown (P< 0.05). All the experimental diets had a pleasant smell (P< 0.05). The texture of diets with 0g FSF/kg was moderately firm while diets 20g FSF/kg, 40g FSF/kg and 60g FSF/kg had a firm texture. The order of preference of diets supplemented with fossil shell flour in terms of feed intake by Dohne Merino rams was: 60g FSF/kg > 40g FSF/kg > 20gFSF/kg > 0g FSF/kg. In the second experiment, a total of twenty-four (n=24) Dohne Merino rams of about 8 to 9 months old, weighing 19.8 ± 0.43 kg were confined in individual pens for a period of 100 days. The rams were subjected to four varying inclusion levels of fossil shell flour (0g/kg, 20g/kg, 40g/kg and 60g/kg. Water and feed intake were recorded throughout the period of successive feeding. Blood samples were collected on days 0, 45 and 90. The parameters studied included the average daily water intake (ADWI), average feed intake (ADFI), skin temperature (ST), respiration rate (RR), pulse rate (PR), rectal temperature (RT), total plasma protein (TPP), mean corpuscular hemoglobin concentration (MCHC) and mean corpuscular volume (MCV). The inclusion levels of FSF affected both the ADWI and ADFI, showing a high-water intake and feed intake in rams subjected to 40g/kg of FSF followed by 60g FSF/kg, 20g FSF/kg and 0g FSF/kg (P< 0.01) respectively. All the physiological parameters ST, RT, RR, HR and PR decreased with an increase in the inclusion levels of FSF (P< 0.01). The TPP increased as the levels of FSF increases (P< 0.01). The MCHC was low in rams subjected to 60g FSF/kg and in 0g to 40g FSF/kg TPP increases as levels of FSF increases (P< 0.01). This study concluded that supplementing with various inclusion levels of FSF had a positive effect on feed preference. The FSF also showed the ability to mitigate heat stress in Dohne Merino rams. , Thesis (MSc) -- Faculty of Science and Agriculture, 2020
- Full Text:
- Date Issued: 2020-02
- Authors: Mwanda, Lwazi https://orcid.org/0000-0001-9503-6150
- Date: 2020-02
- Subjects: Sheep--Feeding and feeds , Animal feeding , Fossils
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/22567 , vital:52464
- Description: The objective of the current study was to evaluate the effects of feeding Dohne Merino rams with various inclusion levels of Fossil Shell Flour (FSF) as an additive supplement on feed preference and heat tolerance response of Dohne Merino rams. The FSF is used as an animal feed additive and is known to be rich in minerals. A total of twenty-four Dohne Merino rams (average 19.8 ± 0.43 kg body weight) were used to determine the feed preference of varying inclusion levels fossil shell flour (0g FSF/kg, 20g FSF/kg, 40g FSF/kg and 60g FSF/kg). The preference was assessed using feed intake and coefficient of preference. The feed intake, co-efficient of preference and physical attributes of feed (such as colour, smell and texture) was measured. The feed intake and coefficient of preference were higher in rams supplemented 60g FSF/kg (P < 0.05). The physical attributes of the experimental diets were adjudged based on the colour, smell and texture. The colour of experimental diets were brown in 0g FSF/kg while in 20g FSF/kg, 40g FSF/kg and 60g FSF/kg the colour of experimental diets were light brown (P< 0.05). All the experimental diets had a pleasant smell (P< 0.05). The texture of diets with 0g FSF/kg was moderately firm while diets 20g FSF/kg, 40g FSF/kg and 60g FSF/kg had a firm texture. The order of preference of diets supplemented with fossil shell flour in terms of feed intake by Dohne Merino rams was: 60g FSF/kg > 40g FSF/kg > 20gFSF/kg > 0g FSF/kg. In the second experiment, a total of twenty-four (n=24) Dohne Merino rams of about 8 to 9 months old, weighing 19.8 ± 0.43 kg were confined in individual pens for a period of 100 days. The rams were subjected to four varying inclusion levels of fossil shell flour (0g/kg, 20g/kg, 40g/kg and 60g/kg. Water and feed intake were recorded throughout the period of successive feeding. Blood samples were collected on days 0, 45 and 90. The parameters studied included the average daily water intake (ADWI), average feed intake (ADFI), skin temperature (ST), respiration rate (RR), pulse rate (PR), rectal temperature (RT), total plasma protein (TPP), mean corpuscular hemoglobin concentration (MCHC) and mean corpuscular volume (MCV). The inclusion levels of FSF affected both the ADWI and ADFI, showing a high-water intake and feed intake in rams subjected to 40g/kg of FSF followed by 60g FSF/kg, 20g FSF/kg and 0g FSF/kg (P< 0.01) respectively. All the physiological parameters ST, RT, RR, HR and PR decreased with an increase in the inclusion levels of FSF (P< 0.01). The TPP increased as the levels of FSF increases (P< 0.01). The MCHC was low in rams subjected to 60g FSF/kg and in 0g to 40g FSF/kg TPP increases as levels of FSF increases (P< 0.01). This study concluded that supplementing with various inclusion levels of FSF had a positive effect on feed preference. The FSF also showed the ability to mitigate heat stress in Dohne Merino rams. , Thesis (MSc) -- Faculty of Science and Agriculture, 2020
- Full Text:
- Date Issued: 2020-02
Evaluation of near infrared reflectance (NIR) spectroscopy to determine the nutrient composition of raw materials and compound ostrich feeds
- Swart, Etheresia, Lehmann-Maritz, Maryna
- Authors: Swart, Etheresia , Lehmann-Maritz, Maryna
- Date: 2017
- Subjects: Near infrared spectroscopy , Animal nutrition , Animal feeding
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/13779 , vital:27307
- Description: The chemical analysis of feed samples can be time consuming and expensive. The use of near infrared reflectance (NIR) spectroscopy was evaluated in a range of studies as a rapid technique to predict the chemical constituents in feedstuffs and compound ostrich feeds. The prediction of accurate results by NIR spectroscopy relies heavily upon obtaining a calibration set which represents the variation in the main population, accurate laboratory analyses and the application of the best mathematical procedures. This research project was designed to meet five objectives: The first objective was to determine the feasibility of using near infrared reflectance (NIR) spectroscopy to predict dry matter, ash, crude protein, crude fibre, oil content, and fatty acids such as palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1) and linoleic acid (C18:2) in sunflower seed meal. The second objective was to develop calibration models to predict the dry matter, crude protein and oil content in milled canola seed, compared to whole canola seeds. The third objective was to investigate the feasibility of using NIR spectroscopy to predict the dry matter, ash, crude protein, crude fibre and oil content in milled lupin seeds, compared to whole lupin seeds. The fourth objective was to describe the development of near infrared reflectance (NIR) spectroscopy calibration equations for the prediction of chemical composition and amino acid content from different populations of alfalfa hay (Medicago sativa L.). The last objective was to determine the potential of NIR spectroscopy to predict the dry matter, ash, crude protein, crude fibre, ether extract, acid detergent fibre (ADF), neutral detergent fibre (NDF), calcium, phosphorus, in vitro organic matter digestibility (IVOMD) and amino acids such as lysine, methionine, threonine and arginine in compound ostrich feed samples. The results of this study indicate that NIR spectroscopy calibrations in sunflower seed meal are only applicable in sunflower breeding programmes for a fast screening as it was not suitable for prediction purposes. Screening of sunflower seeds by NIR spectroscopy represents a rapid, simple and cost effective alternative that is a great utility for users who need to analyse a large number of samples. Calibrations developed for crude protein and oil content in milled canola seeds proved to be better than calibrations for whole canola seeds. Although the results indicated that calibrations were better for milled canola seeds, it indicated values that were typical of equations suitable for screening purposes to select samples for more detailed chemical analysis. According to calibration statistics obtained for crude protein, crude fibre and oil content in whole lupin seeds, there is no need to grind the seeds to scan the meal as similarly accurate results were obtained by analysing whole seeds. Screening of whole lupin seeds by NIR spectroscopy represents a rapid, simple and cost effective alternative that may be of great utility for users who need to analyse a large number of samples with no sample preparation. The calibration and validation statistics obtained in the study to test the potential of NIR spectroscopy to predict the chemical composition and amino acid contents in alfalfa hay, showed the accuracy was too low for routine analysis, although NIR spectroscopy could be used as a screening tool. Further research needs to be done to improve the accuracy of the NIR spectroscopy analysis, including more samples from different cultivars and years. In the study to examine the possibility of using NIR spectroscopy to predict the chemical composition of compound ostrich feeds, the results indicated that NIR spectroscopy is a suitable tool for a rapid and reliable prediction of the crude protein, crude fibre, ether extract, IVOMD, ADF and NDF in compound ostrich feeds. Calibrations can be improved for amino acids if a larger sample pool is used to develop the calibrations. These studies indicated that NIR spectroscopy can be a rapid and successful tool for the prediction of the nutritive value up to certain amino acid contents of feedstuffs and compound ostrich feeds.
- Full Text:
- Date Issued: 2017
- Authors: Swart, Etheresia , Lehmann-Maritz, Maryna
- Date: 2017
- Subjects: Near infrared spectroscopy , Animal nutrition , Animal feeding
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/13779 , vital:27307
- Description: The chemical analysis of feed samples can be time consuming and expensive. The use of near infrared reflectance (NIR) spectroscopy was evaluated in a range of studies as a rapid technique to predict the chemical constituents in feedstuffs and compound ostrich feeds. The prediction of accurate results by NIR spectroscopy relies heavily upon obtaining a calibration set which represents the variation in the main population, accurate laboratory analyses and the application of the best mathematical procedures. This research project was designed to meet five objectives: The first objective was to determine the feasibility of using near infrared reflectance (NIR) spectroscopy to predict dry matter, ash, crude protein, crude fibre, oil content, and fatty acids such as palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1) and linoleic acid (C18:2) in sunflower seed meal. The second objective was to develop calibration models to predict the dry matter, crude protein and oil content in milled canola seed, compared to whole canola seeds. The third objective was to investigate the feasibility of using NIR spectroscopy to predict the dry matter, ash, crude protein, crude fibre and oil content in milled lupin seeds, compared to whole lupin seeds. The fourth objective was to describe the development of near infrared reflectance (NIR) spectroscopy calibration equations for the prediction of chemical composition and amino acid content from different populations of alfalfa hay (Medicago sativa L.). The last objective was to determine the potential of NIR spectroscopy to predict the dry matter, ash, crude protein, crude fibre, ether extract, acid detergent fibre (ADF), neutral detergent fibre (NDF), calcium, phosphorus, in vitro organic matter digestibility (IVOMD) and amino acids such as lysine, methionine, threonine and arginine in compound ostrich feed samples. The results of this study indicate that NIR spectroscopy calibrations in sunflower seed meal are only applicable in sunflower breeding programmes for a fast screening as it was not suitable for prediction purposes. Screening of sunflower seeds by NIR spectroscopy represents a rapid, simple and cost effective alternative that is a great utility for users who need to analyse a large number of samples. Calibrations developed for crude protein and oil content in milled canola seeds proved to be better than calibrations for whole canola seeds. Although the results indicated that calibrations were better for milled canola seeds, it indicated values that were typical of equations suitable for screening purposes to select samples for more detailed chemical analysis. According to calibration statistics obtained for crude protein, crude fibre and oil content in whole lupin seeds, there is no need to grind the seeds to scan the meal as similarly accurate results were obtained by analysing whole seeds. Screening of whole lupin seeds by NIR spectroscopy represents a rapid, simple and cost effective alternative that may be of great utility for users who need to analyse a large number of samples with no sample preparation. The calibration and validation statistics obtained in the study to test the potential of NIR spectroscopy to predict the chemical composition and amino acid contents in alfalfa hay, showed the accuracy was too low for routine analysis, although NIR spectroscopy could be used as a screening tool. Further research needs to be done to improve the accuracy of the NIR spectroscopy analysis, including more samples from different cultivars and years. In the study to examine the possibility of using NIR spectroscopy to predict the chemical composition of compound ostrich feeds, the results indicated that NIR spectroscopy is a suitable tool for a rapid and reliable prediction of the crude protein, crude fibre, ether extract, IVOMD, ADF and NDF in compound ostrich feeds. Calibrations can be improved for amino acids if a larger sample pool is used to develop the calibrations. These studies indicated that NIR spectroscopy can be a rapid and successful tool for the prediction of the nutritive value up to certain amino acid contents of feedstuffs and compound ostrich feeds.
- Full Text:
- Date Issued: 2017
Quantitative evaluation of starch determination in feed samples using Near Infra-red Reflectance
- Authors: Mapekula, Nwabisa Nolwazi
- Date: 2012
- Subjects: Near infrared spectroscopy , Animal feeding , Feeds -- Enzyme content
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10421 , http://hdl.handle.net/10948/d1015617
- Description: In an attempt to reduce the costs and turn around times experienced with outsourcing starch analysis in finished feed products, the company decided to invest in a NIR instrument to carry out such analyses in house. The particular instrument purchased by the company comes with a build in calibration for specific finished feed products and the main objective of this investigation was to evaluate whether the instrumentation and build in calibration gives reliable analyses of a finished poultry feed. The results obtained showed that : - The NIR results obtained have a positive bias compared to the theoretical (formulation specified value) and also to the value obtained by enzymatic starch analysis. - Despite the positive bias, the starch values were well within the allowable limits - Repeatability measurements on the data generated by two different analysts showed that while the percentage relative standard deviations obtained (< 1.0 percent) were well within the company specifications of percentage RSD < 5.0 percent, the inter sample repeatability showed small, but significant variation. - The results for intermediate precision showed that there is no statistically significant difference between the results obtained by two different analysts, nor was there any difference in the results of the same samples analysed at different time periods. - Reproducibility could not be evaluated due to the lack of another instrument within the company that were similarly set up as the instrument used for the investigation. This investigation has shown that, given the restrictions enforced by the build incalibration of the NIR instrument, the use of NIR for the analyses of the finished feed product is valid, but will require that careful attention be paid to data collection procedures.
- Full Text:
- Date Issued: 2012
- Authors: Mapekula, Nwabisa Nolwazi
- Date: 2012
- Subjects: Near infrared spectroscopy , Animal feeding , Feeds -- Enzyme content
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10421 , http://hdl.handle.net/10948/d1015617
- Description: In an attempt to reduce the costs and turn around times experienced with outsourcing starch analysis in finished feed products, the company decided to invest in a NIR instrument to carry out such analyses in house. The particular instrument purchased by the company comes with a build in calibration for specific finished feed products and the main objective of this investigation was to evaluate whether the instrumentation and build in calibration gives reliable analyses of a finished poultry feed. The results obtained showed that : - The NIR results obtained have a positive bias compared to the theoretical (formulation specified value) and also to the value obtained by enzymatic starch analysis. - Despite the positive bias, the starch values were well within the allowable limits - Repeatability measurements on the data generated by two different analysts showed that while the percentage relative standard deviations obtained (< 1.0 percent) were well within the company specifications of percentage RSD < 5.0 percent, the inter sample repeatability showed small, but significant variation. - The results for intermediate precision showed that there is no statistically significant difference between the results obtained by two different analysts, nor was there any difference in the results of the same samples analysed at different time periods. - Reproducibility could not be evaluated due to the lack of another instrument within the company that were similarly set up as the instrument used for the investigation. This investigation has shown that, given the restrictions enforced by the build incalibration of the NIR instrument, the use of NIR for the analyses of the finished feed product is valid, but will require that careful attention be paid to data collection procedures.
- Full Text:
- Date Issued: 2012
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