Antimicrobial activity of selected plants and their combined preparations against pathogens causing respiratory infections
- Authors: Onyebuchi, Ukwuoma Collins
- Date: 2017
- Subjects: Anti-infective agents , Respiratory infections -- Alternative treatment
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/21197 , vital:29455
- Description: Bacterial infection of the respiratory system is one of the leading causes of death in the world’s population, killing over four million people annually. Treatment of respiratory infections is usually by antibiotics administration. The emergence of multi-drug-resistant pathogens has led to the search for more effective therapeutic agents especially from medicinal plants, as compounds from plants have been shown to possess some therapeutic potential. The aim of this study is to determine the antimicrobial activity of three plants, Tetradenia riparia, Leonotis leonurus and Salvia africana-lutea and the activity of their various combined preparations against 27 pathogens usually associated with respiratory ailments. Various solvents were used for extraction of the dried powdered plant materials. The agar disc diffusion antimicrobial assay and the micro-dilution assay methods were used to determine the inhibitory activity and the minimum inhibitory concentration of the plant extracts. The nature of phytochemicals present in the extracts was determined by thin layer chromatography. The results obtained in this study, showed that ethanol produced the highest average extract yield in both the individual and combined plant preparations. A two-way analysis of variance for the disc diffusion assay showed no significant difference in the inhibition zones (Appendix 1 and 2). MIC of 0.8 mg/ml was obtained with S. africana-lutea against S. pneumoniae ATCC 49619 and B. cereus ATCC 10976 strains, indicating a strong antibacterial activity. Synergistic interaction (FIC index ranging from 0.20 – 0.50) and a strong antimicrobial activity (MIC values ranging from 0.4 – 0.8 mg/ml), was obtained with the acetone extract of the 1:1 combination of L. leonurus with S. africana-lutea. The XEA and CEF solvent systems eluted the most number of compound spots and produced the best separations. The results obtained in this study validate the traditional use of these plants and their combinations for the treatment of respiratory ailments.
- Full Text:
- Date Issued: 2017
- Authors: Onyebuchi, Ukwuoma Collins
- Date: 2017
- Subjects: Anti-infective agents , Respiratory infections -- Alternative treatment
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/21197 , vital:29455
- Description: Bacterial infection of the respiratory system is one of the leading causes of death in the world’s population, killing over four million people annually. Treatment of respiratory infections is usually by antibiotics administration. The emergence of multi-drug-resistant pathogens has led to the search for more effective therapeutic agents especially from medicinal plants, as compounds from plants have been shown to possess some therapeutic potential. The aim of this study is to determine the antimicrobial activity of three plants, Tetradenia riparia, Leonotis leonurus and Salvia africana-lutea and the activity of their various combined preparations against 27 pathogens usually associated with respiratory ailments. Various solvents were used for extraction of the dried powdered plant materials. The agar disc diffusion antimicrobial assay and the micro-dilution assay methods were used to determine the inhibitory activity and the minimum inhibitory concentration of the plant extracts. The nature of phytochemicals present in the extracts was determined by thin layer chromatography. The results obtained in this study, showed that ethanol produced the highest average extract yield in both the individual and combined plant preparations. A two-way analysis of variance for the disc diffusion assay showed no significant difference in the inhibition zones (Appendix 1 and 2). MIC of 0.8 mg/ml was obtained with S. africana-lutea against S. pneumoniae ATCC 49619 and B. cereus ATCC 10976 strains, indicating a strong antibacterial activity. Synergistic interaction (FIC index ranging from 0.20 – 0.50) and a strong antimicrobial activity (MIC values ranging from 0.4 – 0.8 mg/ml), was obtained with the acetone extract of the 1:1 combination of L. leonurus with S. africana-lutea. The XEA and CEF solvent systems eluted the most number of compound spots and produced the best separations. The results obtained in this study validate the traditional use of these plants and their combinations for the treatment of respiratory ailments.
- Full Text:
- Date Issued: 2017
Composition and fate of triclosan in the sludge from wastewater treatment in Grahamstown, South Africa and Tiaret, Algeria
- Authors: Ncube, Mbonisi
- Date: 2017
- Subjects: Sewage sludge , Sewage Purification South Africa Grahamstown , Sewage Purification Algeria Tiaret , Sewage sludge as fertilizer , Anti-infective agents
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/65156 , vital:28697
- Description: Physicochemical properties such as pH, specific surface area (SSA), cationic exchange capacity (CEC), loss on ignition (LOI), pathogens, plant nutrients (nitrates, ammonium and phosphates), and heavy metals (manganese, copper, lead and cadmium) were determined for sewage sludge from Grahamstown and Tiaret. The values obtained were log transformed thereafter a t-test at 5 % level of significance was used to test for the difference in each parameter for both sludges. The pH of sludge was determined in 1:3 water, 16 water, 1:3 0.01 M calcium chloride and 1:3 1 M potassium chloride. The pH for Grahamstown and Tiaret sludge were in the ranges of 6.66-7.11 and 7.88-8.18 respectively. The SSA values for Grahamstown and Tiaret were 218 ± 108 and 261 ± 99.9 m2/g, and the CEC values were 119 ± 2.09 and 136 ± 6.03 mEq/100, respectively. The LOI values obtained were 1.33 ± 0.03 and 1.48 ± 0.11 % for Grahamstown and Tiaret, respectively. E. coll and heterotrophic bacteria were the pathogens determined, and were extracted from sludge using sterile saline and nutrient broth. The concentration of E. coll in Grahamstown and Tiaret sludge were 468 ± 7.63 and 7769 ± 1268 CFU/g d.w and for heterotrophic bacteria were 1.17x109 ± 7.42x108 and 1.43x109 ± 9.11 x108 CFU/g d.w. For Grahamstown sludge, the concentration of nitrates, ammonium and phosphates were 55.61 ± 55.20 mg/g d.w, 6.60 ± 2.36 mg/g d.w and 1.40 ± 0.30 mg/g d.w, respectively. For Tiaret sludge, the concentration of nitrates, ammonium and phosphates were 2.56 ± 2.90 mg/g d.w, 0.64 ± 0.45 mg/g d.w and 0.24 ± 0.19 mg/g d.w, respectively. The concentration of Mn, Cu, Pb and Cd in Grahamstown sludge were 423 ± 101, 353 ± 92, 40.2 ± 20 and 0.0 mg/kg d.w respectively, and for Tiaret sludge, the corresponding concentrations were 358± 295, 549±50, 1427± 1352 and 1.54 ± 0.61 mg/kg d.w. Sewage sludge was found to contain Triclosan, and solubility studies of the compound were conducted using sodium deoxycholate and sodium lithocholate. The apparent solubilities and rate constants indicated in brackets of TCS at 37 °C were 35.4 ± 1.21 mg/L (1.28 ± 0.36 Hr-) and 14.4 ± 0.34 mg/L (0.99 ± 0.17 Hr-) in sodium lithocholate and sodium deoxycholate, respectively. The apparent solubilities and rate constants indicated in brackets of TCS at 15 °C were 32.3 ± 0.88 mg/L (2.16 ± 0.80 Hr-) and 14.2 ± 0.39 mg/L (1.02 ± 0.17 Hr-) in sodium lithocholate and sodium deoxycholate, respectively. Triclosan was extracted from sludge using 1 g/L sodium deoxycholate and the determined concentration were 142 ± 33.5 gg/g d.w for Grahamstown sludge and 0-12 gg/g d.w for Tiaret sludge. Finally plant growth studies were conducted on radish and garden cress plants using Grahamstown sludge at 0, 20, 40, 80 and 100 % treatments. Statistical analysis (t-test and Kruskal-Wallis) at 5 % level of significance was done to compare growth parameters between control and different sludge treatments. For radish plants, the values for plant height, root length, number of leaves, leaf length and dry mass were 28.4-80-7 mm, 4.3-44.7 mm, 3.3-17.0 mm, 2.3-4.0 leaves and 6.3-15.3 %, respectively. For garden cress, the values for plant height, root length, number of leaves, leaf length and dry mass were 13.7-25.0 mm, 7.7-20.3 mm, 5.7-8.3 leaves, 3.0-8.3 mm and 8.8-15.0 %, respectively. Twenty percent (20 %) sludge treatment gave the best results in radish and garden cress plants with respect to plant height, root length, number of leaves and dry mass. Triclosan concentration in radish and garden cress plants was below the detection limit of 32.4 gg/g d.w. , Thesis (MSc) -- Faculty of Pharmacy, Pharmacy, 2017
- Full Text:
- Date Issued: 2017
- Authors: Ncube, Mbonisi
- Date: 2017
- Subjects: Sewage sludge , Sewage Purification South Africa Grahamstown , Sewage Purification Algeria Tiaret , Sewage sludge as fertilizer , Anti-infective agents
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/65156 , vital:28697
- Description: Physicochemical properties such as pH, specific surface area (SSA), cationic exchange capacity (CEC), loss on ignition (LOI), pathogens, plant nutrients (nitrates, ammonium and phosphates), and heavy metals (manganese, copper, lead and cadmium) were determined for sewage sludge from Grahamstown and Tiaret. The values obtained were log transformed thereafter a t-test at 5 % level of significance was used to test for the difference in each parameter for both sludges. The pH of sludge was determined in 1:3 water, 16 water, 1:3 0.01 M calcium chloride and 1:3 1 M potassium chloride. The pH for Grahamstown and Tiaret sludge were in the ranges of 6.66-7.11 and 7.88-8.18 respectively. The SSA values for Grahamstown and Tiaret were 218 ± 108 and 261 ± 99.9 m2/g, and the CEC values were 119 ± 2.09 and 136 ± 6.03 mEq/100, respectively. The LOI values obtained were 1.33 ± 0.03 and 1.48 ± 0.11 % for Grahamstown and Tiaret, respectively. E. coll and heterotrophic bacteria were the pathogens determined, and were extracted from sludge using sterile saline and nutrient broth. The concentration of E. coll in Grahamstown and Tiaret sludge were 468 ± 7.63 and 7769 ± 1268 CFU/g d.w and for heterotrophic bacteria were 1.17x109 ± 7.42x108 and 1.43x109 ± 9.11 x108 CFU/g d.w. For Grahamstown sludge, the concentration of nitrates, ammonium and phosphates were 55.61 ± 55.20 mg/g d.w, 6.60 ± 2.36 mg/g d.w and 1.40 ± 0.30 mg/g d.w, respectively. For Tiaret sludge, the concentration of nitrates, ammonium and phosphates were 2.56 ± 2.90 mg/g d.w, 0.64 ± 0.45 mg/g d.w and 0.24 ± 0.19 mg/g d.w, respectively. The concentration of Mn, Cu, Pb and Cd in Grahamstown sludge were 423 ± 101, 353 ± 92, 40.2 ± 20 and 0.0 mg/kg d.w respectively, and for Tiaret sludge, the corresponding concentrations were 358± 295, 549±50, 1427± 1352 and 1.54 ± 0.61 mg/kg d.w. Sewage sludge was found to contain Triclosan, and solubility studies of the compound were conducted using sodium deoxycholate and sodium lithocholate. The apparent solubilities and rate constants indicated in brackets of TCS at 37 °C were 35.4 ± 1.21 mg/L (1.28 ± 0.36 Hr-) and 14.4 ± 0.34 mg/L (0.99 ± 0.17 Hr-) in sodium lithocholate and sodium deoxycholate, respectively. The apparent solubilities and rate constants indicated in brackets of TCS at 15 °C were 32.3 ± 0.88 mg/L (2.16 ± 0.80 Hr-) and 14.2 ± 0.39 mg/L (1.02 ± 0.17 Hr-) in sodium lithocholate and sodium deoxycholate, respectively. Triclosan was extracted from sludge using 1 g/L sodium deoxycholate and the determined concentration were 142 ± 33.5 gg/g d.w for Grahamstown sludge and 0-12 gg/g d.w for Tiaret sludge. Finally plant growth studies were conducted on radish and garden cress plants using Grahamstown sludge at 0, 20, 40, 80 and 100 % treatments. Statistical analysis (t-test and Kruskal-Wallis) at 5 % level of significance was done to compare growth parameters between control and different sludge treatments. For radish plants, the values for plant height, root length, number of leaves, leaf length and dry mass were 28.4-80-7 mm, 4.3-44.7 mm, 3.3-17.0 mm, 2.3-4.0 leaves and 6.3-15.3 %, respectively. For garden cress, the values for plant height, root length, number of leaves, leaf length and dry mass were 13.7-25.0 mm, 7.7-20.3 mm, 5.7-8.3 leaves, 3.0-8.3 mm and 8.8-15.0 %, respectively. Twenty percent (20 %) sludge treatment gave the best results in radish and garden cress plants with respect to plant height, root length, number of leaves and dry mass. Triclosan concentration in radish and garden cress plants was below the detection limit of 32.4 gg/g d.w. , Thesis (MSc) -- Faculty of Pharmacy, Pharmacy, 2017
- Full Text:
- Date Issued: 2017
Synthesis and physicochemical evaluation of a series of boron dipyrromethene dye derivatives for potential utility in antimicrobial photodynamic therapy and nonlinear optics
- Authors: Kubheka, Gugu Patience
- Date: 2017
- Subjects: Dyes and dyeing -- Chemistry , Photochemotherapy , Cancer -- Photochemotherapy , Anti-infective agents , Nonlinear optics , BODIPY
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4776 , vital:20723
- Description: A series of new BODIPY dye derivatives have been synthesized and characterized using various characterization tools such as 1H-NMR, MALDI-TOF mass spectrometry, FT-IR, UV-visible spectrophotometry and elemental analysis. The aniline-substituted BODIPY derivative was further coordinated with gold nanorods and the characterization was achieved by transmission electron microscopy (TEM), X-ray diffractometry (XRD) and X-ray photoelectron spectroscopy (XPS).In addition to this dye, quaternized BODIPY dyes were also synthesized and investigated for their potential utility as photosentitizers in antimicrobial photodynamic therapy (APDT).BODIPY dyes with pyrene substituted styryl groups were embedded in polymer thin film using poly(bisphenol A carbonate) (PBC) to study their optical limiting properties. The optical limiting values of these BODIPY dyes once embedded in thin films were found to be greatly improved and the limiting intensityof each film was well below the maximum threshold which is set to be 0.95 J.cm-². The physicochemical properties and NLO parameters of all of the synthesized dyes were investigated.
- Full Text:
- Date Issued: 2017
- Authors: Kubheka, Gugu Patience
- Date: 2017
- Subjects: Dyes and dyeing -- Chemistry , Photochemotherapy , Cancer -- Photochemotherapy , Anti-infective agents , Nonlinear optics , BODIPY
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4776 , vital:20723
- Description: A series of new BODIPY dye derivatives have been synthesized and characterized using various characterization tools such as 1H-NMR, MALDI-TOF mass spectrometry, FT-IR, UV-visible spectrophotometry and elemental analysis. The aniline-substituted BODIPY derivative was further coordinated with gold nanorods and the characterization was achieved by transmission electron microscopy (TEM), X-ray diffractometry (XRD) and X-ray photoelectron spectroscopy (XPS).In addition to this dye, quaternized BODIPY dyes were also synthesized and investigated for their potential utility as photosentitizers in antimicrobial photodynamic therapy (APDT).BODIPY dyes with pyrene substituted styryl groups were embedded in polymer thin film using poly(bisphenol A carbonate) (PBC) to study their optical limiting properties. The optical limiting values of these BODIPY dyes once embedded in thin films were found to be greatly improved and the limiting intensityof each film was well below the maximum threshold which is set to be 0.95 J.cm-². The physicochemical properties and NLO parameters of all of the synthesized dyes were investigated.
- Full Text:
- Date Issued: 2017
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