Inhibitory potential of honey on the enzymatic activity of Helicobacter pylori urease
- Authors: Matongo, Fredrick
- Date: 2012
- Subjects: Honey , Helicobacter pylori infections , Enzyme inhibitors , Traditional medicine , Antifungal agents
- Language: English
- Type: Thesis , Masters , MSc (Biochemistry)
- Identifier: vital:11253 , http://hdl.handle.net/10353/431 , Honey , Helicobacter pylori infections , Enzyme inhibitors , Traditional medicine , Antifungal agents
- Description: Urease of Helicobacter pylori is an important virulence factor implicated in the pathogenesis of many clinical conditions, such as chronic gastritis, peptic ulceration, and gastric cancer. Many urease inhibitors have been discovered, like phosphorodiamidates, hydroxamic acid derivatives, and imidazoles. Despite good activities at the enzyme level and excellent kinetic properties most of them have not been used as therapeutic agents in vivo because of their side effects, toxicity and instability. This has led to much attention to focus on exploring the novel urease inhibitory activities of natural products because of their low toxicity and good bioavailability. Honey, a natural product has been used in folk medicine due to its antitumor, antioxidant, antimicrobial and anti-inflammatory properties. The aims of this study were to isolate, characterise, purify urease produced by H. pylori and investigate the inhibitory effects of solvent honey extracts on its enzymatic activity. Urease was found to be both surface-associated and cytoplasmic. Maximum cytoplasmic urease activity was found to occur after 72 hr whereas maximum extracellular urease activities were found to occur after 96 hr. Characterization of the crude cytoplasmic urease revealed optimal activity at a pH of 7.5 and temperature of 40°C. The kinetic parameters Vmax and Km were 45.32 U ml-1 and 61.11 mM respectively.The honey extracts inhibited the activity of the crude urease in a concentration dependent manner. The Lineweaver-Burk plots indicated a non-competitive type of inhibition against H. pylori urease. The two honey extracts gave promising inhibitory activities against urease of H. pylori. Thus the results of this study delineates that inhibition of urease can ease development in therapeutic and preventative approaches based on the enzymatic activity of this Helicobacter protein.
- Full Text:
- Date Issued: 2012
- Authors: Matongo, Fredrick
- Date: 2012
- Subjects: Honey , Helicobacter pylori infections , Enzyme inhibitors , Traditional medicine , Antifungal agents
- Language: English
- Type: Thesis , Masters , MSc (Biochemistry)
- Identifier: vital:11253 , http://hdl.handle.net/10353/431 , Honey , Helicobacter pylori infections , Enzyme inhibitors , Traditional medicine , Antifungal agents
- Description: Urease of Helicobacter pylori is an important virulence factor implicated in the pathogenesis of many clinical conditions, such as chronic gastritis, peptic ulceration, and gastric cancer. Many urease inhibitors have been discovered, like phosphorodiamidates, hydroxamic acid derivatives, and imidazoles. Despite good activities at the enzyme level and excellent kinetic properties most of them have not been used as therapeutic agents in vivo because of their side effects, toxicity and instability. This has led to much attention to focus on exploring the novel urease inhibitory activities of natural products because of their low toxicity and good bioavailability. Honey, a natural product has been used in folk medicine due to its antitumor, antioxidant, antimicrobial and anti-inflammatory properties. The aims of this study were to isolate, characterise, purify urease produced by H. pylori and investigate the inhibitory effects of solvent honey extracts on its enzymatic activity. Urease was found to be both surface-associated and cytoplasmic. Maximum cytoplasmic urease activity was found to occur after 72 hr whereas maximum extracellular urease activities were found to occur after 96 hr. Characterization of the crude cytoplasmic urease revealed optimal activity at a pH of 7.5 and temperature of 40°C. The kinetic parameters Vmax and Km were 45.32 U ml-1 and 61.11 mM respectively.The honey extracts inhibited the activity of the crude urease in a concentration dependent manner. The Lineweaver-Burk plots indicated a non-competitive type of inhibition against H. pylori urease. The two honey extracts gave promising inhibitory activities against urease of H. pylori. Thus the results of this study delineates that inhibition of urease can ease development in therapeutic and preventative approaches based on the enzymatic activity of this Helicobacter protein.
- Full Text:
- Date Issued: 2012
The Sound of Africa: Eastern Congo and Ruanda-Urundi
- Authors: Tracey, Hugh
- Subjects: Lake Tanganyika , Mount Ruwenzori , Ituri forest , Pygmies , Tusti , Gold fields , Kilo Moto , Likembe , Hand piano , Guitar , Popular , Soldiers , Askaris , Lip platters , Trade , Honey , Honey Harvesting , Yodelling , Whistling , Hunting , Buck , Iddi , Clapping , Salt , Fishing , Elephant , Elephant hunting , Tembo , Lullaby , Manatoba kukwo , Rift Valley , Cattle , Herdsmen , Flute , Hills , Batwa , Half-pygmies , Pygmoids , Praise song , Chiefs , Mpundu , Mendicant , Begger , Bow , Ugubu bow , Hutu , Tutsi , Zither , Moral story song , Inanga zither , Umwimbo , Usumbura , Ntore dance , Horns , Drums , Royalty , Hima kings , Kimula , Alu Mbira , Boyoka nalinga , The Elephant Hunt
- Language: English
- Type: Sound , Radio broadcast , Music
- Identifier: vital:15067 , http://hdl.handle.net/10962/d1008495 , Reel numbers: BC041, BC042, BC043, BC044
- Description: 12th programme in ‘The Sound of Africa‘ Series A of illustrated talks by Hugh Tracey on his travels in search of African music, broadcast by the South African Broadcasting Corporation , For further details refer to the ILAM Document Collection: Hugh Tracey Broadcasts
- Full Text: false
- Authors: Tracey, Hugh
- Subjects: Lake Tanganyika , Mount Ruwenzori , Ituri forest , Pygmies , Tusti , Gold fields , Kilo Moto , Likembe , Hand piano , Guitar , Popular , Soldiers , Askaris , Lip platters , Trade , Honey , Honey Harvesting , Yodelling , Whistling , Hunting , Buck , Iddi , Clapping , Salt , Fishing , Elephant , Elephant hunting , Tembo , Lullaby , Manatoba kukwo , Rift Valley , Cattle , Herdsmen , Flute , Hills , Batwa , Half-pygmies , Pygmoids , Praise song , Chiefs , Mpundu , Mendicant , Begger , Bow , Ugubu bow , Hutu , Tutsi , Zither , Moral story song , Inanga zither , Umwimbo , Usumbura , Ntore dance , Horns , Drums , Royalty , Hima kings , Kimula , Alu Mbira , Boyoka nalinga , The Elephant Hunt
- Language: English
- Type: Sound , Radio broadcast , Music
- Identifier: vital:15067 , http://hdl.handle.net/10962/d1008495 , Reel numbers: BC041, BC042, BC043, BC044
- Description: 12th programme in ‘The Sound of Africa‘ Series A of illustrated talks by Hugh Tracey on his travels in search of African music, broadcast by the South African Broadcasting Corporation , For further details refer to the ILAM Document Collection: Hugh Tracey Broadcasts
- Full Text: false
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